spen cDNA ORF clone, Danio rerio(zebrafish)

The following spen gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spen cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa146279 XM_021473688.1
Latest version!
Danio rerio spen family transcriptional repressor (spen), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27765 XM_003201204.5
Latest version!
Danio rerio spen family transcriptional repressor (spen), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa41575 XM_009296917.3
Latest version!
Danio rerio spen family transcriptional repressor (spen), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa41575 XM_009296917.2 Danio rerio spen family transcriptional repressor (spen), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa27765 XM_003201204.4 Danio rerio spen family transcriptional repressor (spen), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa146279
    Clone ID Related Accession (Same CDS sequence) XM_021473688.1
    Accession Version XM_021473688.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product msx2-interacting protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395151.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    ATGGTCCGGG AAACCAGACA TTTATGGGTG GGAAATTTAC CAGAAAATGT ACGAGAGGAA 
    AAAATTATCG AGCACTTCAA ACGGTATGGA CGAGTGGAGA GTGTCAAGGT TCTCCCTAAA
    CGTGGATCGG AAGGTGGAGT GGCAGCTTTT GTGGATTTTG TGGACATTAA GAGTGCTCAG
    AAAGCTCATA ATTCTATCAA CAAGATGGGA GACAGGGACC TTCGCACCGA TTACAATGAA
    CCAGGAACTA TACCCAGTGC AGCTCGAGGC CTGGATGATA GCCTGTCCAT AGCCACTCGT
    GGGCGGGATG TTTCAGGGTT CACAAGGGGG GCAGGGGGCC CTGTGTATGG GCCCTCTGTG
    TCACTTCACA GCAGAGAGGG ACGCTTTGAA CGCAGACTAG ACGGGACTTC GGACAGCCGG
    GAACGCTCTT ACGATCACAG TGCCTATGGA CACCATGAGC GTAGCAGCAG CAACAGCACT
    AGTTTCGATC GGCAGCGTCA CTATGAGAGC GATTATTACA GAGACTCTAG GGACCGGGCC
    CTCAGTGGAA CCAGTGGGAG TGCCAGTTCA GCCAGTGGAA GCATCGGAGG AAGCTCATCT
    GCGGGCAGTA TAGGTGTTGC AGGGAATGTT GCAGGAAGCA GTGGTGCTGG TGGAAGTACT
    GCCGCTGGAG TAGGCAGTGG TGGCTCCACA CCAGGAGGAA TTGTCTATTA TGGTTCACGA
    AGCCGGAGCC CCAGTCGTTT TGAGACTACA GAGACTCGTT ATGAGCCCCG TGCCCGTGAA
    GCTTTCACAC TTGCCAGTGT GGTTCATAGG GATTTATTCA GAGAAGAAAG AGGCAGACGT
    GGGGAAAGGA CATACCACCA CAGTCGCAGT AGATCACCAC ACTCTTCACA GTCACACAAT
    CCATCACCAC AGAGGTTGCA AAGTCAGGCA GCGCGACCGG CACGCTCCCG CAGTGGCTCA
    GGATCCCGCA GTCGCTCCTC CAGCTCAGAC TCCGTCAGCA GTACCAGCAG CAGTGGCAGT
    GGCAGTAGTG ATTCCAGCAG CACCTCCAGT GACCGATCTC CAGCTCGATC TGTTCAGTCC
    ACTGCAGTTC CTGCCCCTTC TACACAGCCA CTACCTTCAC TGGACAAAGA TGAACCTCGT
    AAAAGTTTTG GTATCAAAGT GCAGAACCTG CCCGTGCGCT CAACAGATAC AAGCCTCAAG
    GATGGTCTTT TCCATGAATT TAAGAAACAT GGTAAAGTCA CATCAGTTCA GATCCACGGT
    GCCTCTGAGG AACGATATGG CTTAGTGTTC TTTCGCCAGC AAGAGGACCA AGAAAAAGCT
    CTAAGTGCAT CTAAAGGGAA GCTGTTTTTT GGCATGCAAA TTGATGTCAC TGCTTGGCAT
    GGCCCAGAAA CAGAAAGTGA GAACGAGTTC CGGCCTTTGG ATGAACGCAT AGATGAGTTT
    CATCCCAAAG CTACAAGGAC ATTATTCATA GGAAATTTGG AAAAAACCAC AACCTATAAT
    GACCTGCTGA ACATATTTCA GCGTTTTGGA GAGATTGTTG ATATTGATAT TAAAAAGGTA
    AATGGTTCCC CACAATATGC CTTTCTCCAG TATTGTGACA TAGCAAGTGT CTGTAAAGCA
    ATTAAAAAAA TGGATGGTGA ATATCTTGGC AACAACAGGT TAAAGCTTGG TTTTGGCAAG
    AGTATGCCTA CTACATGTGT ATGGTTGGAT GGCTTGTCCT CCAGTATTAC CGAACAGTAT
    CTCACAAGGC ACTTTTGCCG TTATGGACAT GTAGTAAAGG TTGTCTTTGA CCGGCTGAAA
    GGAATGGCCC TCATCTTATA TAATAATATA GAGTATGCGC AGGCTGCTGT CAAGGAAACA
    AAGGGTTGGA AAATTGGAGG AAATAAAATA AAGGTTGACT TTGCTAACCA GGAAAGTCAG
    ATGGCTTTCT ACCGTTCAAT GCAGGGATCT GGACAAGACA TAAGAGATTT CTATGAAATC
    ATATCTGAAC GAAGAGATGA GCGCCGGACG CCATATCATG AGTTCACAGC TGAACGTGCA
    TACTATGAAA ATGTGCGTAC CCCAACTTCT TACACAGAGG ATCCACGTCG AAAATATCCT
    GCCAGAAGTC GAGAGTTTTT TTCAGAATGG GATCCCTACC AGGGAGATTA CTATGACCCA
    CGATATTATG ATGACTCCCG TGAATACAGG GATTATAGAG ACCCATATGA GCAGGACATT
    AGAAAGTACA GCTACTTGCA AAGAGAACGT GAAAGGGAAA GAGAGAGGTT TGAAACAGAT
    CGTGAGCGAG ACCATGGTCG ACGAACAATT GAACACCAAC AAAGCCCAAC CCATTCACGT
    CGTCCTGCTA GTCCAACTGC TTCTCCATCC CTTTCTGAAC GTCCTCCCAG TGACTCTGAA
    CATCACGTTT ACAGTCGCTC ATCTGAGCGA AGTGGTAGCT GCAGCTCACT TTCTCCACCA
    CGCTTTGAGA AGCCAGACAA AATTCGTTTG GAGAGGCACA ATAGGAATGA TAAATTGGAG
    AAAGAAAAAT CACTTTTTGA GACAGAGCGT GGAAATGGTG GGGAAAAAGA AAGGCGAGCA
    GGACGTAAGG AAAAAGGAGA AAAGGACAGG ACTGAGAGAC AGAAGTTGAA GAAGTTAAAA
    CTTGCATCTC CCACTGTTCC ATCACCAGAG GCAGAGCTGG AACTTGACAG GGATGCAAGC
    CCAGAGGCTA ACATAACTCT GCGAGGTAAA GTGAGCAAAT CATTGATCAA AGACAAAGAC
    TACTCTGGTA AAGGAAAACT TGATCTGCTA CCTTGTGTTG TCCAGCTAAC AAGAGTAAAA
    GAGAAGGAAG GAAAATTAAT AGACAGTATC CTCTGTGAAA AACAAAAAAC AAAGGTTGGA
    AGTGATCCTG TTCTGTCTCC AACAACACCT TCCTCAGGTG ATCACAAGAG TATGCCTTTT
    CGCAAAGACA CTCAGGCTAG AGATTTCTTT AAGCATGGAA AACATCTCAA GGAGAAAGGC
    CTGGCAAGCC AAGTTGAGGT AGTGGATAAA GAGGAAAAAG TGAAAAACAA AAAATATTTC
    AAATCTGATC TTGCATTTGA ATCCAGCTCT TCTGTGGATG CTGACCGCAA GGCTGCACGA
    AAGAGACGCT TTGAAGAAAC ATCTGCTAAG GCTGATAATT TAAGGAGAGT AAGTCAGGAG
    GAGGATGAAG GGAAATTAAG GAGAATTATT GATGAACCTT TGCTGAAAGA CACTGATTAT
    GATAAAAAGT TGCTTCGGAA AGAAACACAC AAGAGAGAGC GAAAAATTAA GCCAGAGAGG
    ATGGTTACTG TTAGTACTAC AATAGAAGAG TTAGACAATG TAATGCCAGT AGGCCTCAGT
    TTAGACCTTC AGGCTCGACT TGGGGAACCA GCTGAAGATG CAATAGATCC ATTAGATAGC
    CTTGATCAAA AGATAGGTGC TTTTGGAGCT AACAGACAAC CAAGTTTCAG TCTTGCAGTC
    TCCGACGATG GAAGTGTAGA TATGGATTTT TCAAGAGAGC AAGAGCAGCA GCATTTAACT
    AGCTACCACA CATTATCCTC AAGGCAAGAA AGAGTTTCTG AAAGTAAGGA AAGTTTGCTC
    TCAGACATTG ATCATTCGCA GAGTTGCAGA AAACAAATGG AGCAAAATCG CCGCTTACAG
    CAGCAAATGC TGGAGTGTGA TAAATCAGAT AAAACTGAAA GCACACCAAG CACTGATGCG
    GAGGAATTTG AGCATCGAAG TATTGTGCAT GAGGTTGTAA AACCATTACA AGATGTAACG
    GGAAGTTCTC CGACCAGCAA GCAAAAAAAA TTGGGAGGAT TCGAATTTGA TTTTGGCAAA
    AGAGAGCAAA ATTATGAAAT GTTTCGACTG AGAAATGATG AACCTGAAAG AGGACTTGCT
    TCACATCCAG GGACACCCCT TGCTGAGGAG GAGAGAAATG CATCACAATT GTTGGACAAA
    GACCCAGATT TACCAGTGAC AATGGATAAA AATAGCTCGC ATTTAGATGT TTCAAAATAC
    AATACTAGCA ATGCAGTGCA TCAAGGGCTT TCACCACATG CAGAGATACT AAAAGTGAAA
    CCATCAGTTA CTAAAGAGGA GTTCAGTTGG GAGAGCAATA TTAGGCAGGG CACATTGAGA
    GAAATGAGCT TTCCCACAAG CATTGTTAAA CGGGAAAGTA TCAGAAAACG TCCTGAACGC
    GAATTAGAAC CTGGAGAGGT TCAGTCAGAC TCTGATGAAG ATGAAAGCAG ACACTATTCA
    TTAAAGCCAA TTTCTTTCAA AAGAGAGCAT GAAGAGCGGC TTTCAGATGT AAAGTATTCT
    GAGTCTCTTG AAAAGAATAA GTTCTATGAA TTTGCATTGG ATAAGACGAT AACACCAGAC
    ACAAAAGCTT TGCTTGAGCG TGCTAAGTCA CTGTCTTCAT CTAGAGAAGA AAATTGGTTT
    TTCCTTAATC ATGACTCAAA ATTCAAAAGT TTACAGAATA ACATCGACAA GGAAAAGTCT
    GAACCTACTC CAAGACCTAT TCCTTCATGG TACTTGAAGA AAAAGAAAAG TCGCTCTGAC
    TCTGATGGAA AACTTGATGA AAAAAAGCCA GATGCTAAGC CTGATGAACA AGAAAGACAA
    GATCTCCTAG CTTCACGATT TCTTCATAGC TCAATCTTTG AACAGGATTC ACGTCGGCTT
    CAACACCTTG AACGGAAAAA TACTGACCCT GACGTTAGAA TTGGCAGAGA TAGTGTTACA
    AGTAATTCTC AAGGTGAACA ACCTGGAACA GGAGGATCAG ACTTGACTCA AGAACCAAGA
    GTACTTTTTC ATAGCCGCTT TTTGGAACTT CAGCAAAGAG ATAAGAACCA ACAGCTTCCT
    ATTTCAGAAA AACTTAGTTT AACTGATCAA ATGGAGAAGA CATTGGATGA AGAGTTTAGG
    CCATCTCCCA ATTTATCAGA CATTTCCCAA GAGTCATTAG CAAGTCATAG CTTAACACCT
    ACAATATACC CAGTGTGTCA GTCTAAAATT TCAGAGATGT CTGTGCACCA TGAGAAGCCT
    GTTTTGCATA CTACTTTACC AAACATACAT AATCCAGCAG TTGTGAATGT TAGGTCAGAA
    AATGCTCCAG TAGATTATCC ATCGTTAATT AGCCTAAAGG ATGAGAACAA CACTATTCCG
    TTGAGCACTC CATCACCTGA GCCCATGATA AAGGAAGATG TAATTGTTGA AGAACCTGAA
    GAAAAACTCA GAGAGTCCAG AAAAACTGTA CACGCCGCTG TTGAACACGA GATTGAGATC
    AAACCTCCCA CTCCTGGTGC ATCTTTAGGT AATGTTGAGC CAGAATGTGA TGCTCTGCAG
    GCATCTTCTC CATTGTATCC AAATCCCAGT AAAGATCAAG AGACTACAGA GCTCTCAGAA
    ACAACAACAG AGCCTGCTAA TTCTTCTCTT GTTGTATCTC CTCTGGAGGT GCCAGACACA
    GAAATTGAGC AAGTTGAACA AGTACTACCA CCGCGTAAAC AACCAAAGAG TAAAAAGGTA
    AAGAATGTCT CATCAGCGCA AACACCAATC ACCCAAGTCG TTAATGAAAA ACCAGCTACA
    CGAAAGAGTG AACGCATTGA TAAAGAAAAG CTAAAACGAT CCCCCAGAGG AGACTCTACA
    AAACTTACTG CTGATTCCAG AAATTCTGCA AAATCTCCTG TTCAAGTTTT AGATTCAGAG
    CAGGGCCTTG AATTAAACAC AAACCAAGGC AGAACACGCC AACGACGGAA TGTGCGATCT
    GTCTATGCAA CACCCCCAGA GGAAGAATCT CCTCAACAAC AAGGAAAAGA GGTAACAGAA
    CCTCCCCGTT CCACTCTTAA GCGCTCTAGG GGTCGTCCAC CCAAGACTCG CAGACGAGTG
    GATGATATGT CTCCGGTAAA AGGAGATCAG ATCAAAAATT CTGAGGCTGA AGACACTGAA
    AATAAAGAGT CAACAAGTAG TGGAGAAATA ACTAAAAGTA CAGAGGCATG GCGCTCTCCA
    CGTTCTCAAA AGGGCCAGTC TTCACCGATA AGATCAGGAC AAAGCAAAAA GACATTGAAA
    TTGGACAAAG TGTCAGGTAC TTCGGCATGT GTTCAATTAG ACAGAGAAGC AGTTGATGTG
    CCACCTGCCC TAGATCGCAA AGCTGAATCC AAAGAAACTT TGATGCAAGT TGGACATTTG
    TCAAAAGATA CCAAACAGAA CAGCATATCT ACAAGAGAAA AAGAACTTGT TGCTGAGGAA
    ATTTCAACAG ATGACCGTGT AGAAATCACT CCTGTAGAAA AAATATTAGA CAAGGAGGTC
    AAGACATCAA GGTCAACACG GAACACAAAA ACAATGTCTG ATAATAAGGC TGTCAATGTG
    GTGAAGCTCA CTATGGATGC AGTTAAGGAA GCTGTTCATT CAGGTGATGA CATAACTGTT
    TGTTTTGAAG GGTCTTTAAA AGCTAATGTT CCACAATCAG TAATAGAACA GTCAGACATT
    CAAGATTATC ACAAAAAGGA AAATATTAAC AGCCTTGATG AAAAGGATGA GACTCTTTCA
    GAAATGGAGC CTCCTACTGA TCCTGTGGCT GCCCTGCTTG CTCGCCAAAT GGAACTGGAA
    AGAGCAGTGG AAAATATTTC AAGATTTACA GATGACCAGC ATCCAGTACC TTACAAAGAA
    CCTCGTACAG AACCACCTAC CCTAACAGCA CCTGTTGTAG TACAGCCTGC AGAGGATACA
    GAGGTAGGAA AGCCTGCCAA TCCAGCAAGT GAAACTGAAT TGGCTGAAGC AATTAATTCA
    ATTACTGCTG AGGATATATC AGGTGATACA GATGGATTCT CTGCTTCAAC AACATACACA
    ACCCTCCTTC CTCAATCAGA GACTCTTGAT TTGCCTGTAC CAAATGAGGT TGTGGAAACT
    GAGTCAGACT TGACTGTAAA GACTATTGCA CAGTCTGAAC AAGATAATGT TATGGTTCCA
    GTTTCAAAAT CTTCTAAATG TAACACAGAT ACTTCACTCA AAGAATCACC ATTGACAGAG
    GTAGCAAAAA GAGGAGGAAG AGCACGTCCA AAAACTGCAA AAAAATCCAG AGGGAGGAAG
    GTTTCTTTGA ACAGAAAATT AGATATTGCT GAGGATGTAG TGCTGGAACC TGAATCCACT
    ACAGTCAAAC TTCCAGAATC GATTCCAGAA GAGATTCAAA CAGTTAACCC TAAGGCAGCA
    ACATCGACAG CTGCAGCAGC TGTAGTCACA GTTGCTGCTG CATGCAAACA TGAAGCTGCA
    TCTACAGTGA TTTTGAATAC ACCAAAAGAG GCAGAACAGC CTGCAGTTGA TCAGCCTGAA
    CCTCAAGAAT CTGCCTTCCA TTCTGGAAAT AACAGTCCTT CTTATTTGAG GACACAACAG
    TCATCTCCTG AGCGAGGAGC ATCTGCCCTT ACTTCACCTA CAACACGCCT AAATTCACCT
    GCATCCTCTA ACATTCCTCC TGAATGGAAT ACCAGGACAG AGGAGAAAGT AATGCTACCT
    AAACCACAAG GTAATGTGGC TTTGTCTATC CCAGGAGCTG GGGGACCCCC AGCAAATCCT
    CCCATGCCTC CTGACACAAA AGCCTCTGAT ATTAATGCAA GTTCTAGTAC ACTGCGAAAA
    ATTTTAATGG AACCAAAGTA TGTTTCTGCT TCAAATAGCA ATTCAGTGAC AAGTATGCAG
    TTTACTACCA CATTAGCAGA TCCACGGATG TGTGATAATG AAAATTCAGT TGAGGCTGTG
    CTGCCTTTAA AAACCTCTCT ACCTGAAGAT AGACCTAGCC CTATAGCTCA GCTTGTACCC
    CGTACAACAC CACCGCAGCC CCCTCCTCTA CAACAATGTG AGACTCCACA GATCTTAAAG
    GAGAAACTAG CTATAACTTC CACTGCCACT TCAGTCATTA GTCGGATCCC TATGCCCTTT
    GATTTTGAGG ACACTCCTCG CATCTCTTTA AGTAATCGTA ACTCTGGAAT GTCTTTGCCA
    AAGCAGAAAT ATCGTACTGG CTTGAATGAA AATAATAGAT ATCATGGACA TAACACTTCT
    GAGGATGGTG GAAGTGTTGG GCGCCCAGTT GTTGAAGGCA CACATTGTAA CACGGGGTCT
    AGTACTGGTT TGAGGGTCAA CACATCTGAG GGTGTGGTAG TATTAAGTTA TTCTGGACAA
    AAGACAGAGG GACCACAGCG GATCATTGCA AAAATTAGCC AAATCCCTCC AGCCAGTGCA
    GTTGACATTG AATTTCAGCA GTCTATAACA AAATCTCAGA TTAAACAAGA ACCTTCATCT
    CACCCCTCTA CCCCAAAAGG ATCACAGACA CCTACAGGCT ATAGTCACAC AGGGGTAGTA
    TTAGCTGGCC AAAGTATCAA TGCCCAACCT GTTATATCCT CAATACGCCA GGAAAGCCCT
    GGCTCTGAAA AATCAGAAAC ATCTTACCAA CAAGGAAGTT CTCTGAAATC TTTCCACCAG
    TCCCCAAGCC ATCCTCAACT TCTGAGGTAT GGTCAGTCAA TAACAACGCA ACACCATATG
    AAGAAAAATG GAGAAACTGA ATCATTTTCA ATGAAAGCTG ATATAAAACC TGATATAAAG
    GCAACACAGA CTTTCAATGT TAAACCAGTT TTAAGTCCAC GCCACCCATC TTTCTCAGGC
    AATCACATTT TAAGTCCAAG TGCTCCACAT GAAAGAGCTG TCCCACAACC CAAGCAAGAT
    TCACATTCTC CAAGGGCATC TAACCATTCC ATGTCACCCT TTCCAAAGGT TTGCCCCCCT
    AACAGCCCCG TTGTTCTGGG ACCAGCTGGC CCTATGTCTC AGTATGTGTC AAATATACAC
    CATGCTGAGC AGTCAGTGAT CATGCCTCCT CATAGTGTCA CCCAGTCTGT GCCCATGAGT
    CATTTGTCCC AAGGGGATGT CCGAGCCAGT ACTCCAACAT TATCTGGAAT AGGTTATGGA
    TTGCGTTCAG AAAATCTCTT ATCACCTCGA TCGGCACCTC CTCAACGTTC CACGACTCCT
    CAACCTGCAG TGATCAGAGA TGTCATATTG AAATCGCATG CAGGCTCGTC TGTTGGTGGC
    CATGTTGTTG AGACGAGCAA TGATGATGCA CAGAATCTCC CACAAGGACT TCGTAGATCT
    TCTGCCCCAC AGTTGCAACA GGAAAGTATG GTAATACAGT CGGAATTCAA AAATCTGCAT
    CACAGGGCAT TACGTCTAGA TCAGTATGCC AGACTTATGC AGCAGCATTT GACTGACCAT
    CCAGGTGTTG TTGAAAGTCG CCAAACTCGA ACTGCAGAGG TGGTGCAGTC ATCTTCACAC
    ATATCATCTG CTTCTTCTAA AGCCTCTTCC ATAGTAAAGA ATGCTCCACA GATTGTAAGG
    GATGGACAAA AAACAGTGGA GCTGAAGATG TCACCCTCCC CTCATTCAGA CAACAGGATA
    ACAGGACACC CACCAGGCTC AGTGATGGTA TCTCCTCAAG GAGTTCAGTT AAATTACCCT
    GGAAATGGAA ATACCATGAA TGAATACTAC AAAGAAATGC GTGGCTTCCA TCCTCAGTAT
    CCAGGTCATT CAGTAATTGG AATTAACTTG GCTAACCGTG GGATTCCTGT GTCTCAGGTC
    TCACAAATTG ATCACAGTCA AAGACACAAG GTTCCCTCTC TCTCTTCCAG TGAGTCTGTG
    GGAAGTTTTA GTGAGTCAAA ACTTGAGGGT TCTCACATTC GACACTCAGG CACCATGGAT
    TTGTCTCATA TATCACGAGT TCAGAGTGAG GCTGGGTCCC CCTCTTATAT TTCTCCAGTG
    ACTATAACAC CCAAACTGGA GTTGGCTATT ACTTTGCAGA AGGGGCCTCA AGGACCTGTT
    TCCAACAAGA TGCCACTTCC TTCTTTAGCT TCTTCACAGA TGCGTTCAGA CTTCAAACTT
    GACCACACAG GACTTCGGTC TGTTGATATG GTGCAGTTGT TAACGAAGTA CCCAATCATT
    TGGCAAGGTC ACCTGGCACT TAAGAATGAC ACTGCAGCAG TGCAGCTGCA CTTTGTGTCT
    GGTAATAATG TTCTGGCTCA TCGTTCACTG CCACCGACAG AAGGAGGTGC CTTTCTTCGC
    ATTGCTCAGA GAATGCGCTT AGAGGCTTCA CAACTGGAAG GAGTTGCGAG ACGCATGACT
    GCTGAAAATG AATACTGCCT TCTGTTGGCA TTGCCTTGTG GATTGGACCA AGAAGATGTC
    CACAACCAGA CCCATGCTCT TAAAACTGGT TTTATTACTT ACCTGCAAGC AAAACAGGCT
    GCTGGAATTA TCAATGTCCC AAACCCAGGC TCAAATCAGC CTGCCTACGT TGTCCAGATT
    TTCCCTCCTT GTGAGTTTTC TGAGAGCCAC CTGTCTCATC TTGCTCCGGA CCTTCTCAAC
    AGCATCTCCA GTATCTCTCC TCATCTTATG ATTGTTATAG CTTCTGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329363.1
    CDS743..11173
    Translation

    Target ORF information:

    RefSeq Version XM_021473688.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spen family transcriptional repressor (spen), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473688.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    ATGGTCCGGG AAACCAGACA TTTATGGGTG GGAAATTTAC CAGAAAATGT ACGAGAGGAA 
    AAAATTATCG AGCACTTCAA ACGGTATGGA CGAGTGGAGA GTGTCAAGGT TCTCCCTAAA
    CGTGGATCGG AAGGTGGAGT GGCAGCTTTT GTGGATTTTG TGGACATTAA GAGTGCTCAG
    AAAGCTCATA ATTCTATCAA CAAGATGGGA GACAGGGACC TTCGCACCGA TTACAATGAA
    CCAGGAACTA TACCCAGTGC AGCTCGAGGC CTGGATGATA GCCTGTCCAT AGCCACTCGT
    GGGCGGGATG TTTCAGGGTT CACAAGGGGG GCAGGGGGCC CTGTGTATGG GCCCTCTGTG
    TCACTTCACA GCAGAGAGGG ACGCTTTGAA CGCAGACTAG ACGGGACTTC GGACAGCCGG
    GAACGCTCTT ACGATCACAG TGCCTATGGA CACCATGAGC GTAGCAGCAG CAACAGCACT
    AGTTTCGATC GGCAGCGTCA CTATGAGAGC GATTATTACA GAGACTCTAG GGACCGGGCC
    CTCAGTGGAA CCAGTGGGAG TGCCAGTTCA GCCAGTGGAA GCATCGGAGG AAGCTCATCT
    GCGGGCAGTA TAGGTGTTGC AGGGAATGTT GCAGGAAGCA GTGGTGCTGG TGGAAGTACT
    GCCGCTGGAG TAGGCAGTGG TGGCTCCACA CCAGGAGGAA TTGTCTATTA TGGTTCACGA
    AGCCGGAGCC CCAGTCGTTT TGAGACTACA GAGACTCGTT ATGAGCCCCG TGCCCGTGAA
    GCTTTCACAC TTGCCAGTGT GGTTCATAGG GATTTATTCA GAGAAGAAAG AGGCAGACGT
    GGGGAAAGGA CATACCACCA CAGTCGCAGT AGATCACCAC ACTCTTCACA GTCACACAAT
    CCATCACCAC AGAGGTTGCA AAGTCAGGCA GCGCGACCGG CACGCTCCCG CAGTGGCTCA
    GGATCCCGCA GTCGCTCCTC CAGCTCAGAC TCCGTCAGCA GTACCAGCAG CAGTGGCAGT
    GGCAGTAGTG ATTCCAGCAG CACCTCCAGT GACCGATCTC CAGCTCGATC TGTTCAGTCC
    ACTGCAGTTC CTGCCCCTTC TACACAGCCA CTACCTTCAC TGGACAAAGA TGAACCTCGT
    AAAAGTTTTG GTATCAAAGT GCAGAACCTG CCCGTGCGCT CAACAGATAC AAGCCTCAAG
    GATGGTCTTT TCCATGAATT TAAGAAACAT GGTAAAGTCA CATCAGTTCA GATCCACGGT
    GCCTCTGAGG AACGATATGG CTTAGTGTTC TTTCGCCAGC AAGAGGACCA AGAAAAAGCT
    CTAAGTGCAT CTAAAGGGAA GCTGTTTTTT GGCATGCAAA TTGATGTCAC TGCTTGGCAT
    GGCCCAGAAA CAGAAAGTGA GAACGAGTTC CGGCCTTTGG ATGAACGCAT AGATGAGTTT
    CATCCCAAAG CTACAAGGAC ATTATTCATA GGAAATTTGG AAAAAACCAC AACCTATAAT
    GACCTGCTGA ACATATTTCA GCGTTTTGGA GAGATTGTTG ATATTGATAT TAAAAAGGTA
    AATGGTTCCC CACAATATGC CTTTCTCCAG TATTGTGACA TAGCAAGTGT CTGTAAAGCA
    ATTAAAAAAA TGGATGGTGA ATATCTTGGC AACAACAGGT TAAAGCTTGG TTTTGGCAAG
    AGTATGCCTA CTACATGTGT ATGGTTGGAT GGCTTGTCCT CCAGTATTAC CGAACAGTAT
    CTCACAAGGC ACTTTTGCCG TTATGGACAT GTAGTAAAGG TTGTCTTTGA CCGGCTGAAA
    GGAATGGCCC TCATCTTATA TAATAATATA GAGTATGCGC AGGCTGCTGT CAAGGAAACA
    AAGGGTTGGA AAATTGGAGG AAATAAAATA AAGGTTGACT TTGCTAACCA GGAAAGTCAG
    ATGGCTTTCT ACCGTTCAAT GCAGGGATCT GGACAAGACA TAAGAGATTT CTATGAAATC
    ATATCTGAAC GAAGAGATGA GCGCCGGACG CCATATCATG AGTTCACAGC TGAACGTGCA
    TACTATGAAA ATGTGCGTAC CCCAACTTCT TACACAGAGG ATCCACGTCG AAAATATCCT
    GCCAGAAGTC GAGAGTTTTT TTCAGAATGG GATCCCTACC AGGGAGATTA CTATGACCCA
    CGATATTATG ATGACTCCCG TGAATACAGG GATTATAGAG ACCCATATGA GCAGGACATT
    AGAAAGTACA GCTACTTGCA AAGAGAACGT GAAAGGGAAA GAGAGAGGTT TGAAACAGAT
    CGTGAGCGAG ACCATGGTCG ACGAACAATT GAACACCAAC AAAGCCCAAC CCATTCACGT
    CGTCCTGCTA GTCCAACTGC TTCTCCATCC CTTTCTGAAC GTCCTCCCAG TGACTCTGAA
    CATCACGTTT ACAGTCGCTC ATCTGAGCGA AGTGGTAGCT GCAGCTCACT TTCTCCACCA
    CGCTTTGAGA AGCCAGACAA AATTCGTTTG GAGAGGCACA ATAGGAATGA TAAATTGGAG
    AAAGAAAAAT CACTTTTTGA GACAGAGCGT GGAAATGGTG GGGAAAAAGA AAGGCGAGCA
    GGACGTAAGG AAAAAGGAGA AAAGGACAGG ACTGAGAGAC AGAAGTTGAA GAAGTTAAAA
    CTTGCATCTC CCACTGTTCC ATCACCAGAG GCAGAGCTGG AACTTGACAG GGATGCAAGC
    CCAGAGGCTA ACATAACTCT GCGAGGTAAA GTGAGCAAAT CATTGATCAA AGACAAAGAC
    TACTCTGGTA AAGGAAAACT TGATCTGCTA CCTTGTGTTG TCCAGCTAAC AAGAGTAAAA
    GAGAAGGAAG GAAAATTAAT AGACAGTATC CTCTGTGAAA AACAAAAAAC AAAGGTTGGA
    AGTGATCCTG TTCTGTCTCC AACAACACCT TCCTCAGGTG ATCACAAGAG TATGCCTTTT
    CGCAAAGACA CTCAGGCTAG AGATTTCTTT AAGCATGGAA AACATCTCAA GGAGAAAGGC
    CTGGCAAGCC AAGTTGAGGT AGTGGATAAA GAGGAAAAAG TGAAAAACAA AAAATATTTC
    AAATCTGATC TTGCATTTGA ATCCAGCTCT TCTGTGGATG CTGACCGCAA GGCTGCACGA
    AAGAGACGCT TTGAAGAAAC ATCTGCTAAG GCTGATAATT TAAGGAGAGT AAGTCAGGAG
    GAGGATGAAG GGAAATTAAG GAGAATTATT GATGAACCTT TGCTGAAAGA CACTGATTAT
    GATAAAAAGT TGCTTCGGAA AGAAACACAC AAGAGAGAGC GAAAAATTAA GCCAGAGAGG
    ATGGTTACTG TTAGTACTAC AATAGAAGAG TTAGACAATG TAATGCCAGT AGGCCTCAGT
    TTAGACCTTC AGGCTCGACT TGGGGAACCA GCTGAAGATG CAATAGATCC ATTAGATAGC
    CTTGATCAAA AGATAGGTGC TTTTGGAGCT AACAGACAAC CAAGTTTCAG TCTTGCAGTC
    TCCGACGATG GAAGTGTAGA TATGGATTTT TCAAGAGAGC AAGAGCAGCA GCATTTAACT
    AGCTACCACA CATTATCCTC AAGGCAAGAA AGAGTTTCTG AAAGTAAGGA AAGTTTGCTC
    TCAGACATTG ATCATTCGCA GAGTTGCAGA AAACAAATGG AGCAAAATCG CCGCTTACAG
    CAGCAAATGC TGGAGTGTGA TAAATCAGAT AAAACTGAAA GCACACCAAG CACTGATGCG
    GAGGAATTTG AGCATCGAAG TATTGTGCAT GAGGTTGTAA AACCATTACA AGATGTAACG
    GGAAGTTCTC CGACCAGCAA GCAAAAAAAA TTGGGAGGAT TCGAATTTGA TTTTGGCAAA
    AGAGAGCAAA ATTATGAAAT GTTTCGACTG AGAAATGATG AACCTGAAAG AGGACTTGCT
    TCACATCCAG GGACACCCCT TGCTGAGGAG GAGAGAAATG CATCACAATT GTTGGACAAA
    GACCCAGATT TACCAGTGAC AATGGATAAA AATAGCTCGC ATTTAGATGT TTCAAAATAC
    AATACTAGCA ATGCAGTGCA TCAAGGGCTT TCACCACATG CAGAGATACT AAAAGTGAAA
    CCATCAGTTA CTAAAGAGGA GTTCAGTTGG GAGAGCAATA TTAGGCAGGG CACATTGAGA
    GAAATGAGCT TTCCCACAAG CATTGTTAAA CGGGAAAGTA TCAGAAAACG TCCTGAACGC
    GAATTAGAAC CTGGAGAGGT TCAGTCAGAC TCTGATGAAG ATGAAAGCAG ACACTATTCA
    TTAAAGCCAA TTTCTTTCAA AAGAGAGCAT GAAGAGCGGC TTTCAGATGT AAAGTATTCT
    GAGTCTCTTG AAAAGAATAA GTTCTATGAA TTTGCATTGG ATAAGACGAT AACACCAGAC
    ACAAAAGCTT TGCTTGAGCG TGCTAAGTCA CTGTCTTCAT CTAGAGAAGA AAATTGGTTT
    TTCCTTAATC ATGACTCAAA ATTCAAAAGT TTACAGAATA ACATCGACAA GGAAAAGTCT
    GAACCTACTC CAAGACCTAT TCCTTCATGG TACTTGAAGA AAAAGAAAAG TCGCTCTGAC
    TCTGATGGAA AACTTGATGA AAAAAAGCCA GATGCTAAGC CTGATGAACA AGAAAGACAA
    GATCTCCTAG CTTCACGATT TCTTCATAGC TCAATCTTTG AACAGGATTC ACGTCGGCTT
    CAACACCTTG AACGGAAAAA TACTGACCCT GACGTTAGAA TTGGCAGAGA TAGTGTTACA
    AGTAATTCTC AAGGTGAACA ACCTGGAACA GGAGGATCAG ACTTGACTCA AGAACCAAGA
    GTACTTTTTC ATAGCCGCTT TTTGGAACTT CAGCAAAGAG ATAAGAACCA ACAGCTTCCT
    ATTTCAGAAA AACTTAGTTT AACTGATCAA ATGGAGAAGA CATTGGATGA AGAGTTTAGG
    CCATCTCCCA ATTTATCAGA CATTTCCCAA GAGTCATTAG CAAGTCATAG CTTAACACCT
    ACAATATACC CAGTGTGTCA GTCTAAAATT TCAGAGATGT CTGTGCACCA TGAGAAGCCT
    GTTTTGCATA CTACTTTACC AAACATACAT AATCCAGCAG TTGTGAATGT TAGGTCAGAA
    AATGCTCCAG TAGATTATCC ATCGTTAATT AGCCTAAAGG ATGAGAACAA CACTATTCCG
    TTGAGCACTC CATCACCTGA GCCCATGATA AAGGAAGATG TAATTGTTGA AGAACCTGAA
    GAAAAACTCA GAGAGTCCAG AAAAACTGTA CACGCCGCTG TTGAACACGA GATTGAGATC
    AAACCTCCCA CTCCTGGTGC ATCTTTAGGT AATGTTGAGC CAGAATGTGA TGCTCTGCAG
    GCATCTTCTC CATTGTATCC AAATCCCAGT AAAGATCAAG AGACTACAGA GCTCTCAGAA
    ACAACAACAG AGCCTGCTAA TTCTTCTCTT GTTGTATCTC CTCTGGAGGT GCCAGACACA
    GAAATTGAGC AAGTTGAACA AGTACTACCA CCGCGTAAAC AACCAAAGAG TAAAAAGGTA
    AAGAATGTCT CATCAGCGCA AACACCAATC ACCCAAGTCG TTAATGAAAA ACCAGCTACA
    CGAAAGAGTG AACGCATTGA TAAAGAAAAG CTAAAACGAT CCCCCAGAGG AGACTCTACA
    AAACTTACTG CTGATTCCAG AAATTCTGCA AAATCTCCTG TTCAAGTTTT AGATTCAGAG
    CAGGGCCTTG AATTAAACAC AAACCAAGGC AGAACACGCC AACGACGGAA TGTGCGATCT
    GTCTATGCAA CACCCCCAGA GGAAGAATCT CCTCAACAAC AAGGAAAAGA GGTAACAGAA
    CCTCCCCGTT CCACTCTTAA GCGCTCTAGG GGTCGTCCAC CCAAGACTCG CAGACGAGTG
    GATGATATGT CTCCGGTAAA AGGAGATCAG ATCAAAAATT CTGAGGCTGA AGACACTGAA
    AATAAAGAGT CAACAAGTAG TGGAGAAATA ACTAAAAGTA CAGAGGCATG GCGCTCTCCA
    CGTTCTCAAA AGGGCCAGTC TTCACCGATA AGATCAGGAC AAAGCAAAAA GACATTGAAA
    TTGGACAAAG TGTCAGGTAC TTCGGCATGT GTTCAATTAG ACAGAGAAGC AGTTGATGTG
    CCACCTGCCC TAGATCGCAA AGCTGAATCC AAAGAAACTT TGATGCAAGT TGGACATTTG
    TCAAAAGATA CCAAACAGAA CAGCATATCT ACAAGAGAAA AAGAACTTGT TGCTGAGGAA
    ATTTCAACAG ATGACCGTGT AGAAATCACT CCTGTAGAAA AAATATTAGA CAAGGAGGTC
    AAGACATCAA GGTCAACACG GAACACAAAA ACAATGTCTG ATAATAAGGC TGTCAATGTG
    GTGAAGCTCA CTATGGATGC AGTTAAGGAA GCTGTTCATT CAGGTGATGA CATAACTGTT
    TGTTTTGAAG GGTCTTTAAA AGCTAATGTT CCACAATCAG TAATAGAACA GTCAGACATT
    CAAGATTATC ACAAAAAGGA AAATATTAAC AGCCTTGATG AAAAGGATGA GACTCTTTCA
    GAAATGGAGC CTCCTACTGA TCCTGTGGCT GCCCTGCTTG CTCGCCAAAT GGAACTGGAA
    AGAGCAGTGG AAAATATTTC AAGATTTACA GATGACCAGC ATCCAGTACC TTACAAAGAA
    CCTCGTACAG AACCACCTAC CCTAACAGCA CCTGTTGTAG TACAGCCTGC AGAGGATACA
    GAGGTAGGAA AGCCTGCCAA TCCAGCAAGT GAAACTGAAT TGGCTGAAGC AATTAATTCA
    ATTACTGCTG AGGATATATC AGGTGATACA GATGGATTCT CTGCTTCAAC AACATACACA
    ACCCTCCTTC CTCAATCAGA GACTCTTGAT TTGCCTGTAC CAAATGAGGT TGTGGAAACT
    GAGTCAGACT TGACTGTAAA GACTATTGCA CAGTCTGAAC AAGATAATGT TATGGTTCCA
    GTTTCAAAAT CTTCTAAATG TAACACAGAT ACTTCACTCA AAGAATCACC ATTGACAGAG
    GTAGCAAAAA GAGGAGGAAG AGCACGTCCA AAAACTGCAA AAAAATCCAG AGGGAGGAAG
    GTTTCTTTGA ACAGAAAATT AGATATTGCT GAGGATGTAG TGCTGGAACC TGAATCCACT
    ACAGTCAAAC TTCCAGAATC GATTCCAGAA GAGATTCAAA CAGTTAACCC TAAGGCAGCA
    ACATCGACAG CTGCAGCAGC TGTAGTCACA GTTGCTGCTG CATGCAAACA TGAAGCTGCA
    TCTACAGTGA TTTTGAATAC ACCAAAAGAG GCAGAACAGC CTGCAGTTGA TCAGCCTGAA
    CCTCAAGAAT CTGCCTTCCA TTCTGGAAAT AACAGTCCTT CTTATTTGAG GACACAACAG
    TCATCTCCTG AGCGAGGAGC ATCTGCCCTT ACTTCACCTA CAACACGCCT AAATTCACCT
    GCATCCTCTA ACATTCCTCC TGAATGGAAT ACCAGGACAG AGGAGAAAGT AATGCTACCT
    AAACCACAAG GTAATGTGGC TTTGTCTATC CCAGGAGCTG GGGGACCCCC AGCAAATCCT
    CCCATGCCTC CTGACACAAA AGCCTCTGAT ATTAATGCAA GTTCTAGTAC ACTGCGAAAA
    ATTTTAATGG AACCAAAGTA TGTTTCTGCT TCAAATAGCA ATTCAGTGAC AAGTATGCAG
    TTTACTACCA CATTAGCAGA TCCACGGATG TGTGATAATG AAAATTCAGT TGAGGCTGTG
    CTGCCTTTAA AAACCTCTCT ACCTGAAGAT AGACCTAGCC CTATAGCTCA GCTTGTACCC
    CGTACAACAC CACCGCAGCC CCCTCCTCTA CAACAATGTG AGACTCCACA GATCTTAAAG
    GAGAAACTAG CTATAACTTC CACTGCCACT TCAGTCATTA GTCGGATCCC TATGCCCTTT
    GATTTTGAGG ACACTCCTCG CATCTCTTTA AGTAATCGTA ACTCTGGAAT GTCTTTGCCA
    AAGCAGAAAT ATCGTACTGG CTTGAATGAA AATAATAGAT ATCATGGACA TAACACTTCT
    GAGGATGGTG GAAGTGTTGG GCGCCCAGTT GTTGAAGGCA CACATTGTAA CACGGGGTCT
    AGTACTGGTT TGAGGGTCAA CACATCTGAG GGTGTGGTAG TATTAAGTTA TTCTGGACAA
    AAGACAGAGG GACCACAGCG GATCATTGCA AAAATTAGCC AAATCCCTCC AGCCAGTGCA
    GTTGACATTG AATTTCAGCA GTCTATAACA AAATCTCAGA TTAAACAAGA ACCTTCATCT
    CACCCCTCTA CCCCAAAAGG ATCACAGACA CCTACAGGCT ATAGTCACAC AGGGGTAGTA
    TTAGCTGGCC AAAGTATCAA TGCCCAACCT GTTATATCCT CAATACGCCA GGAAAGCCCT
    GGCTCTGAAA AATCAGAAAC ATCTTACCAA CAAGGAAGTT CTCTGAAATC TTTCCACCAG
    TCCCCAAGCC ATCCTCAACT TCTGAGGTAT GGTCAGTCAA TAACAACGCA ACACCATATG
    AAGAAAAATG GAGAAACTGA ATCATTTTCA ATGAAAGCTG ATATAAAACC TGATATAAAG
    GCAACACAGA CTTTCAATGT TAAACCAGTT TTAAGTCCAC GCCACCCATC TTTCTCAGGC
    AATCACATTT TAAGTCCAAG TGCTCCACAT GAAAGAGCTG TCCCACAACC CAAGCAAGAT
    TCACATTCTC CAAGGGCATC TAACCATTCC ATGTCACCCT TTCCAAAGGT TTGCCCCCCT
    AACAGCCCCG TTGTTCTGGG ACCAGCTGGC CCTATGTCTC AGTATGTGTC AAATATACAC
    CATGCTGAGC AGTCAGTGAT CATGCCTCCT CATAGTGTCA CCCAGTCTGT GCCCATGAGT
    CATTTGTCCC AAGGGGATGT CCGAGCCAGT ACTCCAACAT TATCTGGAAT AGGTTATGGA
    TTGCGTTCAG AAAATCTCTT ATCACCTCGA TCGGCACCTC CTCAACGTTC CACGACTCCT
    CAACCTGCAG TGATCAGAGA TGTCATATTG AAATCGCATG CAGGCTCGTC TGTTGGTGGC
    CATGTTGTTG AGACGAGCAA TGATGATGCA CAGAATCTCC CACAAGGACT TCGTAGATCT
    TCTGCCCCAC AGTTGCAACA GGAAAGTATG GTAATACAGT CGGAATTCAA AAATCTGCAT
    CACAGGGCAT TACGTCTAGA TCAGTATGCC AGACTTATGC AGCAGCATTT GACTGACCAT
    CCAGGTGTTG TTGAAAGTCG CCAAACTCGA ACTGCAGAGG TGGTGCAGTC ATCTTCACAC
    ATATCATCTG CTTCTTCTAA AGCCTCTTCC ATAGTAAAGA ATGCTCCACA GATTGTAAGG
    GATGGACAAA AAACAGTGGA GCTGAAGATG TCACCCTCCC CTCATTCAGA CAACAGGATA
    ACAGGACACC CACCAGGCTC AGTGATGGTA TCTCCTCAAG GAGTTCAGTT AAATTACCCT
    GGAAATGGAA ATACCATGAA TGAATACTAC AAAGAAATGC GTGGCTTCCA TCCTCAGTAT
    CCAGGTCATT CAGTAATTGG AATTAACTTG GCTAACCGTG GGATTCCTGT GTCTCAGGTC
    TCACAAATTG ATCACAGTCA AAGACACAAG GTTCCCTCTC TCTCTTCCAG TGAGTCTGTG
    GGAAGTTTTA GTGAGTCAAA ACTTGAGGGT TCTCACATTC GACACTCAGG CACCATGGAT
    TTGTCTCATA TATCACGAGT TCAGAGTGAG GCTGGGTCCC CCTCTTATAT TTCTCCAGTG
    ACTATAACAC CCAAACTGGA GTTGGCTATT ACTTTGCAGA AGGGGCCTCA AGGACCTGTT
    TCCAACAAGA TGCCACTTCC TTCTTTAGCT TCTTCACAGA TGCGTTCAGA CTTCAAACTT
    GACCACACAG GACTTCGGTC TGTTGATATG GTGCAGTTGT TAACGAAGTA CCCAATCATT
    TGGCAAGGTC ACCTGGCACT TAAGAATGAC ACTGCAGCAG TGCAGCTGCA CTTTGTGTCT
    GGTAATAATG TTCTGGCTCA TCGTTCACTG CCACCGACAG AAGGAGGTGC CTTTCTTCGC
    ATTGCTCAGA GAATGCGCTT AGAGGCTTCA CAACTGGAAG GAGTTGCGAG ACGCATGACT
    GCTGAAAATG AATACTGCCT TCTGTTGGCA TTGCCTTGTG GATTGGACCA AGAAGATGTC
    CACAACCAGA CCCATGCTCT TAAAACTGGT TTTATTACTT ACCTGCAAGC AAAACAGGCT
    GCTGGAATTA TCAATGTCCC AAACCCAGGC TCAAATCAGC CTGCCTACGT TGTCCAGATT
    TTCCCTCCTT GTGAGTTTTC TGAGAGCCAC CTGTCTCATC TTGCTCCGGA CCTTCTCAAC
    AGCATCTCCA GTATCTCTCC TCATCTTATG ATTGTTATAG CTTCTGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27765
    Clone ID Related Accession (Same CDS sequence) XM_003201204.5 , XM_003201204.4
    Accession Version XM_003201204.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product msx2-interacting protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003201204.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    ATGGTCCGGG AAACCAGACA TTTATGGGTG GGAAATTTAC CAGAAAATGT ACGAGAGGAA 
    AAAATTATCG AGCACTTCAA ACGGTATGGA CGAGTGGAGA GTGTCAAGGT TCTCCCTAAA
    CGTGGATCGG AAGGTGGAGT GGCAGCTTTT GTGGATTTTG TGGACATTAA GAGTGCTCAG
    AAAGCTCATA ATTCTATCAA CAAGATGGGA GACAGGGACC TTCGCACCGA TTACAATGAA
    CCAGGAACTA TACCCAGTGC AGCTCGAGGC CTGGATGATA GCCTGTCCAT AGCCACTCGT
    GGGCGGGATG TTTCAGGGTT CACAAGGGGG GCAGGGGGCC CTGTGTATGG GCCCTCTGTG
    TCACTTCACA GCAGAGAGGG ACGCTTTGAA CGCAGACTAG ACGGGACTTC GGACAGCCGG
    GAACGCTCTT ACGATCACAG TGCCTATGGA CACCATGAGC GTAGCAGCAG CAACAGCACT
    AGTTTCGATC GGCAGCGTCA CTATGAGAGC GATTATTACA GAGACTCTAG GGACCGGGCC
    CTCAGTGGAA CCAGTGGGAG TGCCAGTTCA GCCAGTGGAA GCATCGGAGG AAGCTCATCT
    GCGGGCAGTA TAGGTGTTGC AGGGAATGTT GCAGGAAGCA GTGGTGCTGG TGGAAGTACT
    GCCGCTGGAG TAGGCAGTGG TGGCTCCACA CCAGGAGGAA TTGTCTATTA TGGTTCACGA
    AGCCGGAGCC CCAGTCGTTT TGAGACTACA GAGACTCGTT ATGAGCCCCG TGCCCGTGAA
    GCTTTCACAC TTGCCAGTGT GGTTCATCGG GATTTATTCA GAGAAGAAAG AGGCAGACGT
    GGGGAAAGGA CATACCACCA CAGTCGCAGT AGATCACCAC ACTCTTCACA GTCACACAAT
    CCATCACCAC AGAGGTTGCA AAGTCAGGCA GCGCGACCGG CACGCTCCCG CAGTGGCTCA
    GGATCCCGCA GTCGCTCCTC CAGCTCAGAC TCCGTCAGCA GTACCAGCAG CAGTGGCAGT
    GGCAGTAGTG ATTCCAGCAG CACCTCCAGT GACCGATCTC CAGCTCGATC TGTTCAGTCC
    ACTGCAGTTC CTGCCCCTTC TACACAGCCA CTACCTTCAC TGGACAAAGA TGAACCTCGT
    AAAAGTTTTG GTATCAAAGT GCAGAACCTG CCCGTGCGCT CAACAGATAC AAGCCTCAAG
    GATGGTCTTT TCCATGAATT TAAGAAACAT GGTAAAGTCA CATCAGTTCA GATCCACGGT
    GCCTCTGAGG AACGATATGG CTTAGTGTTC TTTCGCCAGC AAGAGGACCA AGAAAAAGCT
    CTAAGTGCAT CTAAAGGGAA GCTGTTTTTT GGCATGCAAA TTGATGTCAC TGCTTGGCAT
    GGCCCAGAAA CAGAAAGTGA GAACGAGTTC CGGCCTTTGG ATGAACGCAT AGATGAGTTT
    CATCCCAAAG CTACAAGGAC ATTGTTCATA GGAAATTTGG AAAAAACCAC AACCTATAAT
    GACCTGCTGA ACATATTTCA GCGTTTTGGA GAGATTGTTG ATATTGATAT TAAAAAGGTA
    AATGGTTCCC CACAATATGC CTTTCTCCAG TATTGTGACA TAGCAAGTGT CTGCAAAGCA
    ATTAAGAAAA TGGATGGTGA ATATCTTGGC AACAACAGGT TAAAGCTTGG TTTTGGCAAG
    AGTATGCCTA CTACATGTGT ATGGTTGGAT GGCTTGTCCT CCAGTATTAC TGAACAGTAT
    CTCACGAGGC ACTTTTGTCG TTATGGACAT GTAGTAAAGG TTGTCTTTGA CCGGCTGAAA
    GGAATGGCCC TCATCTTATA TAATAATATA GAGTATGCGC AGGCTGCTGT CAAGGAAACA
    AAGGGTTGGA AAATTGGAGG AAATAAAATA AAGGTTGACT TTGCTAACCA GGAAAGTCAG
    ATGGCTTTCT ACCGTTCAAT GCAGGGATCT GGACAAGACA TAAGAGATTT CTATGAAATC
    ATATCTGAAC GAAGAGATGA GCGCCGGACG CCATATCACG AGTTCACAGC TGAACGTGCA
    TACTATGAAA ATGTGCGTAC CCCAACTTCT TACACAGAGG ATCCACGTCG AAAATATCCT
    GCCAGAAGTC GAGAGTTTTT TTCAGAATGG GATCCCTACC AGGGAGATTA CTATGACCCA
    CGATATTATG ATGACTCCCG TGAATACAGG GATTTTAGAG ACCCATATGA GCAGGACATT
    AGAAAGTACA GCTACTTGCA AAGAGAACGT GAAAGGGAAA GAGAGAGGTT TGAAACAGAT
    CGTGAGCGAG ACCATGGTCG ACGAACAATT GAACACCAAC AAAGCCCAAC CCATTCACGT
    CGTCCTGCTA GTCCAACTGC TTCTCCATCC CTTTCTGAAC GTCCTCCCAG TGACTCTGAA
    CATCACGTTT ACAGTCGCTC ATCTGAGCGA AGTGGTAGCT GCAGCTCACT TTCTCCACCA
    CGCTTTGAGA AGCCAGACAA AATTCGTTTG GAGAGGCACA ATAGGAATGA TAAATTGGAG
    AAAGAAAAAT CACTTTTTGA GACAGAGCGT GGAAATGGTG GGGAAAAAGA AAGGCGAGCA
    GGACGTAAGG AAAAAGGAGA AAAGGACAGG ACTGAGAGAC AGAAGTTGAA GAAGTTAAAA
    CTTGCATCTC CCACTGTTCC ATCACCAGAG GCAGAGCTGG AACTTGACAG GGATGCAAGC
    CCAGAGGCTA ACATAACTCT GCGAGGTAAA GTGAGCAAAT CATTGATCAA AGACAAAGAC
    TACTCTGGTA AAGGAAAACT TGATCTGCTA CCTTGTGTTG TCCAGCTAAC AAGAGTAAAA
    GAGAAGGAAG GAAAATTAAT AGACAGTATC CTCTGTGAAA AACAAAAAAC AAAGGTTGGA
    AGTGATCCTG TTCTGTCTCC AACAACACCT TCCTCAGGTG ATCACAAGAG TATGCCTTTT
    CGCAAAGACA CTCAGGCTAG AGATTTCTTT AAGCATGGAA AACATCTCAA GGAGAAAGGC
    CTGGCAAGCC AAGTTGAGGT AGTGGATAAA GAGGAAAAAG TGAAAAACAA AAAATATTTC
    AAATCTGATC TTGCATTTGA ATCCAGCTCT TCTGTGGATG CTGACCGCAA GGCTGCACGA
    AAGAGACGCT TTGAAGAAAC ATCGGCTAAG GCTGATAATT TAAGGAGAGT AAGTCAGGAG
    GAGGATGAAG GGAAATTAAG GAGAATTATT GATGAACCTT TGCTGAAAGA CACTGATTAT
    GATAAAAAGT TGCTTCGGAA AGAAGCACAC AAGAGAGAGC GAAAAATTAA ACCAGAGAGG
    ATGGTTACTG TTAGTACTAC AATAGAAGAG TTAGACAATG TAATGCCAGT AGGCCCCAGT
    TTAGACCTTC AGGCTCGACT TGGGGAACCA GCTGAAGATG CAATAGATCC ATTAGATAGC
    CTTGATCAAA AGATAGGTGC TTTTGGAGCT AACAGACAAC CAAGTTTCAG TCTTGCAGTC
    TCCGACGATG GAAGTGTAGA TATGGATTTT TCAAGAGAGC AAGAGCAGCA GCATTTAACT
    AGCTACCACA CATTATCCTC AAGGCAAGAA AGAGTTTCTG AAAGTAAGGA AAGTTTGCTC
    TCAGACATTG ATCATTCGCA GAGTTGCAGA AAACAAATGG AGCAAAATCG CCGCTTACAG
    CAGCAAATGC TGGAGTGTGA TAAATCAGAT AAAACTGAAA GCACACCAAG CACTGATGCG
    GAGGAATTTG AGCATCGAAG TATTGTGCAT GAGGTTGTAA AACCATTACA AGATGTAACG
    GGAAGTTCTC CGACCAGCAA GCAAAAAAAA TTGGGAGGAT TCGAATTTGA TTTTGGCAAA
    AGAGAGCAAA ATTATGAAAT GTTTCGACTG AGAAATGATG AACCTGAAAG AGGACTTGCT
    TCACATCCAG GGACACCCCT TGCTGAGGAG GAGAGAAATG CATCACAATT GTTGGACAAA
    GACCCAGATT TACCAGTGAC AATGGATAAA AATAGCTCGC ATTTAGATGT TTCAAAATAC
    AATACTAGCA ATGCAGTGCA TCAAGGGCTT TCACCACATG CAGAGATACT AAAAGTGAAA
    CCATCAGTTA CTAAAGAGGA GTTCAGTTGG GAGAGCAATA TTAGGCAGGG CACATTGAGA
    GAAATGAGCT TTCCCACAAG CATTGTTAAA CGGGAAAGTA TCAGAAAACG TCCTGAACGC
    GAATTAGAAC CTGGAGAGGT TCAGTCAGAC TCTGATGAAG ATGAAAGCAG ACACTATTCA
    TTAAAGCCAA TTTCTTTCAA AAGAGAGCAT GAAGAGCGGC TTTCAGATGT AAAGTATTCT
    GAGTCTCTTG AAAAGAATAA GTTCTATGAA TTTGCATTGG ATAAGACGAT AACACCAGAC
    ACAAAAGCTT TGCTTGAGCG TGCTAAGTCA CTGTCTTCAT CTAGAGAAGA AAATTGGTTT
    TTCCTTAATC ATGACTCAAA ATTCAAAAGT TTACAGAATA ACATCGACAA GGAAAAGTCT
    GAACCTACTC CAAGACCTAT TCCTTCATGG TACTTGAAGA AAAAGAAAAG TCGCTCTGAC
    TCTGATGGAA AACTTGATGA AAAAAAGCAA GATGCTAAGC CTGATGAACA AGAAAGACAA
    GATCTCCTAG CTTCACGATT TCTTCATAGC TCAATCTTTG AACAGGATTC ACGTCGGCTT
    CAACACCTTG AACGGAAAAA TACTGACCCT GACGTTAGAA TTGGCAGAGA TAGTGTTACA
    AGTAATTCTC AAGGTGAACA ACCTGGAACA GGAGGATCAG ACTTGACTCA AGAACCAAGA
    GTACTTTTTC ATAGCCGCTT TTTGGAACTT CAGCAAAGAG ACAAGAACCA ACAGCTTCCT
    ATTTCAGAAA AACTTAGTTT AACTGATCAA ATGGAGAAGA CATTGGATGA AGAGTTTAGG
    CCATCTCCCA ATTTATCAGA CATTTCCCAA GAGTCATTAG CAAGTCATAG CTTAACACCT
    ACAATATCCC CAGTGTCTCA GTCTAAAATT TCAGAGATGT CTGTGCACCA TGAGAAGCCT
    GTTTTGCATA CTACTTTACC AAACATACAT AATCCAGCAG TTGTGAATGT TAGGTCAGAA
    AATGCTCCAA TAGATTATCC ATCGTTAATT AGCCTAAAGG ATGAGAACAA CACTATTCCG
    TTGAGCACTC CATCACCTGA GCCCATGATA AAGGAAGATG TAATTGTTGA AGAACCTGAA
    GAAAAACTCA GAGATTCCAG AAAAACTGTA CACGCCGCTG TTGAACACGA GATTGAGATC
    AAACCTCCCA CTCCTGGTGC ATCTTTAGGT AATGTTGAGC CAGAATGTGA TGCTCTGCAG
    GCATCTTCTC CATTGTATCC AAATCCCAGT AAAGATCAAG AGACTACAGA GCTCTCAGAA
    ACAACAACAG AGCCTGCTAA TTCTTCTCTT GTTGTATCTC CTCTGGAGGT GCCAGACACA
    GAAATTGAGC AAGTTGAACA AGTACTACCA CCGCGTAAAC AACCAAAGAG TAAAAAGGTA
    AAGAATGTCT CATCAGCGCA AACACCAATC ACCCAAGTCG TTAATGAAAA ACCAGCTACA
    CGAAAGAGTG AACGCATTGA TAAAGAAAAG CTAAAACGAT CCCCCAGAGG AGACTCTACA
    AAACTTACTG CTGATTCCAG AAATTCTGCA AAATCTCCTG TTCAAGTTTT AGATTCAGAG
    CAGGGCCTTG AATTAAACAC AAACCAAGGC AGAACACGCC AACGACGGAA TGTGCGATCT
    GTCTATGCAA CACCCCCAGA GGAAGAATCT CCTCAACAAC AAGGAAAAGA GGTAACAGAA
    CCTCCCCGTT CCACTCTTAA GCGCTCTAGG GGTCGTCCAC CCAAGACTCG CAGACGAGTG
    GATGATATGT CTCCGGTAAA AGGAGATCAG ATCAAAAATT CTGAGGCTGA AGACACTGAA
    AATAAAGAGT CAACAAGTAG TGGAGAAATA ACTAAAAGTA CAGAGGCATG GCGCTCTCCA
    CGTTCTCAAA AGGGCCAGTC TTCACCGATA AGATCAGGAC AAAGCAAAAA GACATTGAAA
    TTGGACAAAG TGTCAGGTAC TTCGGCATGT GTTCAATTAG ACAGAGAAGC AGTTGATGTG
    CCACCTGCCC TAGATCGCAA AGCTGAATCC AAAGAAACTT TGATGCAAGT TGGACATTTG
    TCAAAAGATA CCAAACAGAA CAGCATATCT ACAAGAGAAA AAGAACTTGT TGCTGAGGAA
    ATTTCAACAG ATGACCGTGT AGAAATCACT CCTGTAGAAA AAATATTAGA CAAGGAGGTC
    AAGACATCAA GGTCAACACG GAACACAAAA ACAATGTCTG ATAATAAGGC TGTCAATGTG
    GTGAAGCTCA CTATGGATGC AGTTAAGGAA GCTGTTCATT CAGGTGATGA CATAACTGTT
    TGTTTTGAAG GGTCTTTAAA AGCTAATGTT CCACAATCAG TAATAGAACA GTCAGACATT
    CAAGATTATC ACAAAAAGGA AAATGTTAAC AGCCTTGATG AAAAGGATGA GACTCTTTCA
    GAAATGGAGC CTCCTACTGA TCCTGTGGCT GCCCTGCTTG CTCGCCAAAT GGAACTGGAA
    AGAGCAGTGG AAAATATTTC AAGATTTACA GATGACCAGC ATCCAGTACC TTACAAAGAA
    CCTCGTACAG AACCACCTAC CCTAACAGCA CCTGTTGTAG TACAACCTGC AGAGGATACA
    GAGGTAGGAA AGCCTGCCAA TCCAGCAAGT GAAACTGAAT TGGCTGAAGC AATTAATTCA
    ATTACTGCTG AGGATATATC AGGTGATACA GATGGATTCT CTGCTTCAAC AACATACACA
    ACCCTCCTTC CTCAACCAGA GACTCTTGAT TTGCCTGTAC CAAATGAGGT TGTGGAAACT
    GAGTCAGACT TGACTGTAAA GACTATTGCA CAGTCTGAAC AAGATAATGT TATGGTTCCA
    GTTTCAAAAT CTTCTAAATG TAAAACAGAT ACTTCACTCA AAGAATCACC ATTGACAGAG
    GTAGCAAAAA GAGGAGGAAG AGCACGTCCA AAAACTGCAA AAAAATCCAG AGGGAGGAAG
    GTTTCTTTGA ACAGAAAATT AGATATTGCT GAGGATGTAG TGCTGGAACC TGAATCCACT
    ACAGTCAAAC TTCCAGAATC GATTCCAGAA GAGATTCAAA CAGTTAACCC TAAGGCAGCA
    ACATCGGCAG CTGCAGCAGC TGTAGTCACA GTTGCTGCTG CATGCAAACA TGAAGCTGCA
    TCTACAGTGA TTTTGAATAC ACCAAAAGAG GCAGAACAGC CTGCAGTTGA TCAGCCTGAA
    CCTCAAGAAT CTGCCTTCCA TTCTGGAAAT AACAGTCCTT CTTATTTGAG GACACAACAG
    TCATCTCCTG AGCGAGGAGC ATCTGCCCTT ACTTCACCTA CAACACGCCT AAATTCACCT
    GCATCCTCTA ACATTCCTCC TGAATGGAAT ACCAGGACAG AGGAGAAAGT AATGCTACCT
    AAACCACAAG GTAATGTGGC TTTGTCTATC CCAGGAGCTG GGGGACCCCC AGCAAATCCT
    CCCATGCCTC CTGACACAAA AGCCTCTGAT ATTAATGCAA GTTCTAGTAC ACTGCGAAAA
    ATTTTAATGG AACCAAAGTA TGTTTCTGCT TCAAATAGCA ATTCAGTGAC AAGTATGCAG
    TTTACTACCA CATTAGCAGA TCCACGGATG TCTGATAATG AAAATTCAGT TGAGGCTGTG
    CTGCCTTTAA AAACCTCTCT ACCTGAAGAT AGACCTAGCC CTATAGCTCA GCTTGTACCC
    CGTACAACAC CACCGCAGCC CCCTCCTCTA CAACAATGTG AGACTCCACA GATCTTAAAG
    GAGAAACTAG CTATAACTTC CACTGCCACT TCAGTCATTA GTCGGATCCC TATGCCCTTT
    GATTTTGAGG ACACTCCTCG CATCTCTTTA AGTAATCGTA ACTCTGGAAT GTCTTTGCCA
    AAGCAGAAAT ATCGTACTGG CTTGAATGAA AATAATAGAT ATCATGGACA TAATACTTCT
    GAGGATGGTG GAAGTGTTGG GCGCCCAGTT GTTGAAGGCA CACATTGTAA CACGGGGTCT
    AGTACTGGTT TGAGGGTCAA CACATCTGAG GGTGTGGTAG TATTAAGTTA TTCTGGACAA
    AAGACAGAGG GACCACAGCG GATCATTGCA AAAATTAGCC AAATCCCTCC AGCCAGTGCA
    GTTGACATTG AATTTCAGCA GTCTATAACA AAATCTCAGA TTAAACAAGA ACCTTCATCT
    CACCCCTCTA CCCCAAAAGG ATCACAGACA CCTACAGGCT ATAGTCACAC AGGGGTAGTA
    TTAGCTGGCC AAAGTATCAA TGCCCAACCT GTTATATCCT CAATACGCCA GGAAAGCCCT
    GGCTCTGAAA AATCAGAAAC ATCTTACCAA CAAGGAAGTT CTCTGAAATC TTTCCACCAG
    TCCCCAAGCC ATCCTCAACT TCTGAGGTAT GGTCAGTCAA TAACACCGCA ACACCATATG
    AAGAAAAATG GAGAAACTGA ATCATTTTCA ATGAAAGCTG ATATAAAACC TGATATAAAG
    GCAACACAGA CTTTCAATGT TAAACCAGTT TTAAGTCCAC GCCACCCATC TTTCTCAGGC
    AATCACATTT TAAGTCCAAG TGCTCCACAT GAAAGAGCTG TCCCACAACC CAAGCAAGAT
    TCACATTCTC CAAGGGCATC TAACCATTCC ATGTCACCCT TTCCAAAGGT TTGCCCCCCT
    AACAGCCCCG TTGTTCTGGG ACCAGCTGGC CCTATGTCTC AGTATGTGTC AAATATACAC
    CATGCTGAGC AGTCAGTGAT CATGCCTCCT CATAGTGTCA CCCAGTCTGT GCCCATGAGT
    CATTTGTCCC AAGGGGATGT CCGAGCCAGT ACTCCAACAT TATCTGGAAT AGGTTATGGA
    TTGCGTTCAG AAAATCTCTT ATCACCTCGA TCGGCACCTC CTCAACGTTC CACGACTCCT
    CAACCTGCAG TGATCAGAGA TGTCATATTG AAATCGCATG CAGGCTCGTC TGTTGGTGGC
    CATGTTGTTG AGACGAGCAA TGATGATGCA CAGAATCTCC CACAAGGACT TCGTAGATCT
    TCTGCCCCAC AGTTGCAACA GGAAAGTATG GTAATACAGT CGGAATTCAA AAATCTGCAT
    CACAGGGCAT TACGTCTAGA TCAGTATGCC AGACTTATGC AGCAGCATTT GACTGACCAT
    CCAGGTGTTG TTGAAAGTCG CCAAACTCGA ACTGCAGAGG TGGTGCAGTC ATCTTCACAC
    ATATCATCTG CTTCTTCTAA AGCCTCTTCC ATAGTAAAGA ATGCTCCACA GATTGTAAGG
    GATGGACAAA AAACAGTGGA GCTGAAGATG TCACCCTCCC CTCATTCAGA CAACAGGATA
    ACAGGACACC CACCAGGCTC AGTGATGGTA TCTCCTCAAG GAGTTCAGTT AAATTACCCT
    GGAAATGGAA ATACCATGAA TGAATACTAC AAAGAAATGC GTGGCTTCCA TCCTCAGTAT
    CCAGGTCATT CAGTAATTGG AATTAACTTG GCTAACCGTG GGATTCCTGT GTCTCAGGTC
    TCACAAATTG ATCACAGTCA AAGACACAAG GTTCCCTCTC TCTCTTCCAG TGAGTCTGTG
    GGAAGTTTTA GTGAGTCAAA ACTTGAGGGT TCTCACATTC GACACTCAGG CACCATGGAT
    TTGTCTCATA TATCACGAGT TCAGAGTGAG GCTGGGTCCC CCTCTTATAT TTCTCCAGTG
    ACTATAACAC CCAAACTGGA GTTGGCTATT ACTTTGCAGA AGGGGCCTCA AGGACCTGTT
    TCCAACAAGA TGCCACTTCC TTCTTTAGCT TCTTCACAGA TGCGTTCAGA CTTCAAACTT
    GACCACACAG GACTTCGGTC TGTTGATATG GTGCAGTTGT TAACGAAGTA CCCAATCATT
    TGGCAAGGTC ACCTGGCACT TAAGAATGAC ACTGCAGCAG TGCAGCTGCA CTTTGTGTCT
    GGTAATAATG TTCTGGCTCA TCGTTCACTG CCACCCCCAG AAGGAGGTGC CTTTCTTCGC
    ATTGCTCAGA GAATGCGCTT AGAGGCTTCA CAACTGGAAG GAGTTGCGAG ACGCATGACT
    GCTGAAAATG AATACTGCCT TCTGTTGGCA TTGCCTTGTG GATTGGACCA AGAAGATGTC
    CACAACCAGA CCCATGCTCT TAAAACTGGT TTTATTACTT ACCTGCAAGC AAAACAGGCT
    GCTGGAATTA TCAATGTCCC AAACCCAGGC TCAAATCAGC CTGCCTACGT TGTCCAGATT
    TTCCCTCCTT GTGAGTTTTC TGAGAGCCAC CTGTCTCATC TTGCTCCGGA CCTTCTCAAC
    AGCATCTCCA GTATCTCTCC TCATCTTATG ATTGTTATAG CTTCTGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003201252.2
    CDS1371..11801
    Translation

    Target ORF information:

    RefSeq Version XM_003201204.5
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spen family transcriptional repressor (spen), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003201204.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    ATGGTCCGGG AAACCAGACA TTTATGGGTG GGAAATTTAC CAGAAAATGT ACGAGAGGAA 
    AAAATTATCG AGCACTTCAA ACGGTATGGA CGAGTGGAGA GTGTCAAGGT TCTCCCTAAA
    CGTGGATCGG AAGGTGGAGT GGCAGCTTTT GTGGATTTTG TGGACATTAA GAGTGCTCAG
    AAAGCTCATA ATTCTATCAA CAAGATGGGA GACAGGGACC TTCGCACCGA TTACAATGAA
    CCAGGAACTA TACCCAGTGC AGCTCGAGGC CTGGATGATA GCCTGTCCAT AGCCACTCGT
    GGGCGGGATG TTTCAGGGTT CACAAGGGGG GCAGGGGGCC CTGTGTATGG GCCCTCTGTG
    TCACTTCACA GCAGAGAGGG ACGCTTTGAA CGCAGACTAG ACGGGACTTC GGACAGCCGG
    GAACGCTCTT ACGATCACAG TGCCTATGGA CACCATGAGC GTAGCAGCAG CAACAGCACT
    AGTTTCGATC GGCAGCGTCA CTATGAGAGC GATTATTACA GAGACTCTAG GGACCGGGCC
    CTCAGTGGAA CCAGTGGGAG TGCCAGTTCA GCCAGTGGAA GCATCGGAGG AAGCTCATCT
    GCGGGCAGTA TAGGTGTTGC AGGGAATGTT GCAGGAAGCA GTGGTGCTGG TGGAAGTACT
    GCCGCTGGAG TAGGCAGTGG TGGCTCCACA CCAGGAGGAA TTGTCTATTA TGGTTCACGA
    AGCCGGAGCC CCAGTCGTTT TGAGACTACA GAGACTCGTT ATGAGCCCCG TGCCCGTGAA
    GCTTTCACAC TTGCCAGTGT GGTTCATCGG GATTTATTCA GAGAAGAAAG AGGCAGACGT
    GGGGAAAGGA CATACCACCA CAGTCGCAGT AGATCACCAC ACTCTTCACA GTCACACAAT
    CCATCACCAC AGAGGTTGCA AAGTCAGGCA GCGCGACCGG CACGCTCCCG CAGTGGCTCA
    GGATCCCGCA GTCGCTCCTC CAGCTCAGAC TCCGTCAGCA GTACCAGCAG CAGTGGCAGT
    GGCAGTAGTG ATTCCAGCAG CACCTCCAGT GACCGATCTC CAGCTCGATC TGTTCAGTCC
    ACTGCAGTTC CTGCCCCTTC TACACAGCCA CTACCTTCAC TGGACAAAGA TGAACCTCGT
    AAAAGTTTTG GTATCAAAGT GCAGAACCTG CCCGTGCGCT CAACAGATAC AAGCCTCAAG
    GATGGTCTTT TCCATGAATT TAAGAAACAT GGTAAAGTCA CATCAGTTCA GATCCACGGT
    GCCTCTGAGG AACGATATGG CTTAGTGTTC TTTCGCCAGC AAGAGGACCA AGAAAAAGCT
    CTAAGTGCAT CTAAAGGGAA GCTGTTTTTT GGCATGCAAA TTGATGTCAC TGCTTGGCAT
    GGCCCAGAAA CAGAAAGTGA GAACGAGTTC CGGCCTTTGG ATGAACGCAT AGATGAGTTT
    CATCCCAAAG CTACAAGGAC ATTGTTCATA GGAAATTTGG AAAAAACCAC AACCTATAAT
    GACCTGCTGA ACATATTTCA GCGTTTTGGA GAGATTGTTG ATATTGATAT TAAAAAGGTA
    AATGGTTCCC CACAATATGC CTTTCTCCAG TATTGTGACA TAGCAAGTGT CTGCAAAGCA
    ATTAAGAAAA TGGATGGTGA ATATCTTGGC AACAACAGGT TAAAGCTTGG TTTTGGCAAG
    AGTATGCCTA CTACATGTGT ATGGTTGGAT GGCTTGTCCT CCAGTATTAC TGAACAGTAT
    CTCACGAGGC ACTTTTGTCG TTATGGACAT GTAGTAAAGG TTGTCTTTGA CCGGCTGAAA
    GGAATGGCCC TCATCTTATA TAATAATATA GAGTATGCGC AGGCTGCTGT CAAGGAAACA
    AAGGGTTGGA AAATTGGAGG AAATAAAATA AAGGTTGACT TTGCTAACCA GGAAAGTCAG
    ATGGCTTTCT ACCGTTCAAT GCAGGGATCT GGACAAGACA TAAGAGATTT CTATGAAATC
    ATATCTGAAC GAAGAGATGA GCGCCGGACG CCATATCACG AGTTCACAGC TGAACGTGCA
    TACTATGAAA ATGTGCGTAC CCCAACTTCT TACACAGAGG ATCCACGTCG AAAATATCCT
    GCCAGAAGTC GAGAGTTTTT TTCAGAATGG GATCCCTACC AGGGAGATTA CTATGACCCA
    CGATATTATG ATGACTCCCG TGAATACAGG GATTTTAGAG ACCCATATGA GCAGGACATT
    AGAAAGTACA GCTACTTGCA AAGAGAACGT GAAAGGGAAA GAGAGAGGTT TGAAACAGAT
    CGTGAGCGAG ACCATGGTCG ACGAACAATT GAACACCAAC AAAGCCCAAC CCATTCACGT
    CGTCCTGCTA GTCCAACTGC TTCTCCATCC CTTTCTGAAC GTCCTCCCAG TGACTCTGAA
    CATCACGTTT ACAGTCGCTC ATCTGAGCGA AGTGGTAGCT GCAGCTCACT TTCTCCACCA
    CGCTTTGAGA AGCCAGACAA AATTCGTTTG GAGAGGCACA ATAGGAATGA TAAATTGGAG
    AAAGAAAAAT CACTTTTTGA GACAGAGCGT GGAAATGGTG GGGAAAAAGA AAGGCGAGCA
    GGACGTAAGG AAAAAGGAGA AAAGGACAGG ACTGAGAGAC AGAAGTTGAA GAAGTTAAAA
    CTTGCATCTC CCACTGTTCC ATCACCAGAG GCAGAGCTGG AACTTGACAG GGATGCAAGC
    CCAGAGGCTA ACATAACTCT GCGAGGTAAA GTGAGCAAAT CATTGATCAA AGACAAAGAC
    TACTCTGGTA AAGGAAAACT TGATCTGCTA CCTTGTGTTG TCCAGCTAAC AAGAGTAAAA
    GAGAAGGAAG GAAAATTAAT AGACAGTATC CTCTGTGAAA AACAAAAAAC AAAGGTTGGA
    AGTGATCCTG TTCTGTCTCC AACAACACCT TCCTCAGGTG ATCACAAGAG TATGCCTTTT
    CGCAAAGACA CTCAGGCTAG AGATTTCTTT AAGCATGGAA AACATCTCAA GGAGAAAGGC
    CTGGCAAGCC AAGTTGAGGT AGTGGATAAA GAGGAAAAAG TGAAAAACAA AAAATATTTC
    AAATCTGATC TTGCATTTGA ATCCAGCTCT TCTGTGGATG CTGACCGCAA GGCTGCACGA
    AAGAGACGCT TTGAAGAAAC ATCGGCTAAG GCTGATAATT TAAGGAGAGT AAGTCAGGAG
    GAGGATGAAG GGAAATTAAG GAGAATTATT GATGAACCTT TGCTGAAAGA CACTGATTAT
    GATAAAAAGT TGCTTCGGAA AGAAGCACAC AAGAGAGAGC GAAAAATTAA ACCAGAGAGG
    ATGGTTACTG TTAGTACTAC AATAGAAGAG TTAGACAATG TAATGCCAGT AGGCCCCAGT
    TTAGACCTTC AGGCTCGACT TGGGGAACCA GCTGAAGATG CAATAGATCC ATTAGATAGC
    CTTGATCAAA AGATAGGTGC TTTTGGAGCT AACAGACAAC CAAGTTTCAG TCTTGCAGTC
    TCCGACGATG GAAGTGTAGA TATGGATTTT TCAAGAGAGC AAGAGCAGCA GCATTTAACT
    AGCTACCACA CATTATCCTC AAGGCAAGAA AGAGTTTCTG AAAGTAAGGA AAGTTTGCTC
    TCAGACATTG ATCATTCGCA GAGTTGCAGA AAACAAATGG AGCAAAATCG CCGCTTACAG
    CAGCAAATGC TGGAGTGTGA TAAATCAGAT AAAACTGAAA GCACACCAAG CACTGATGCG
    GAGGAATTTG AGCATCGAAG TATTGTGCAT GAGGTTGTAA AACCATTACA AGATGTAACG
    GGAAGTTCTC CGACCAGCAA GCAAAAAAAA TTGGGAGGAT TCGAATTTGA TTTTGGCAAA
    AGAGAGCAAA ATTATGAAAT GTTTCGACTG AGAAATGATG AACCTGAAAG AGGACTTGCT
    TCACATCCAG GGACACCCCT TGCTGAGGAG GAGAGAAATG CATCACAATT GTTGGACAAA
    GACCCAGATT TACCAGTGAC AATGGATAAA AATAGCTCGC ATTTAGATGT TTCAAAATAC
    AATACTAGCA ATGCAGTGCA TCAAGGGCTT TCACCACATG CAGAGATACT AAAAGTGAAA
    CCATCAGTTA CTAAAGAGGA GTTCAGTTGG GAGAGCAATA TTAGGCAGGG CACATTGAGA
    GAAATGAGCT TTCCCACAAG CATTGTTAAA CGGGAAAGTA TCAGAAAACG TCCTGAACGC
    GAATTAGAAC CTGGAGAGGT TCAGTCAGAC TCTGATGAAG ATGAAAGCAG ACACTATTCA
    TTAAAGCCAA TTTCTTTCAA AAGAGAGCAT GAAGAGCGGC TTTCAGATGT AAAGTATTCT
    GAGTCTCTTG AAAAGAATAA GTTCTATGAA TTTGCATTGG ATAAGACGAT AACACCAGAC
    ACAAAAGCTT TGCTTGAGCG TGCTAAGTCA CTGTCTTCAT CTAGAGAAGA AAATTGGTTT
    TTCCTTAATC ATGACTCAAA ATTCAAAAGT TTACAGAATA ACATCGACAA GGAAAAGTCT
    GAACCTACTC CAAGACCTAT TCCTTCATGG TACTTGAAGA AAAAGAAAAG TCGCTCTGAC
    TCTGATGGAA AACTTGATGA AAAAAAGCAA GATGCTAAGC CTGATGAACA AGAAAGACAA
    GATCTCCTAG CTTCACGATT TCTTCATAGC TCAATCTTTG AACAGGATTC ACGTCGGCTT
    CAACACCTTG AACGGAAAAA TACTGACCCT GACGTTAGAA TTGGCAGAGA TAGTGTTACA
    AGTAATTCTC AAGGTGAACA ACCTGGAACA GGAGGATCAG ACTTGACTCA AGAACCAAGA
    GTACTTTTTC ATAGCCGCTT TTTGGAACTT CAGCAAAGAG ACAAGAACCA ACAGCTTCCT
    ATTTCAGAAA AACTTAGTTT AACTGATCAA ATGGAGAAGA CATTGGATGA AGAGTTTAGG
    CCATCTCCCA ATTTATCAGA CATTTCCCAA GAGTCATTAG CAAGTCATAG CTTAACACCT
    ACAATATCCC CAGTGTCTCA GTCTAAAATT TCAGAGATGT CTGTGCACCA TGAGAAGCCT
    GTTTTGCATA CTACTTTACC AAACATACAT AATCCAGCAG TTGTGAATGT TAGGTCAGAA
    AATGCTCCAA TAGATTATCC ATCGTTAATT AGCCTAAAGG ATGAGAACAA CACTATTCCG
    TTGAGCACTC CATCACCTGA GCCCATGATA AAGGAAGATG TAATTGTTGA AGAACCTGAA
    GAAAAACTCA GAGATTCCAG AAAAACTGTA CACGCCGCTG TTGAACACGA GATTGAGATC
    AAACCTCCCA CTCCTGGTGC ATCTTTAGGT AATGTTGAGC CAGAATGTGA TGCTCTGCAG
    GCATCTTCTC CATTGTATCC AAATCCCAGT AAAGATCAAG AGACTACAGA GCTCTCAGAA
    ACAACAACAG AGCCTGCTAA TTCTTCTCTT GTTGTATCTC CTCTGGAGGT GCCAGACACA
    GAAATTGAGC AAGTTGAACA AGTACTACCA CCGCGTAAAC AACCAAAGAG TAAAAAGGTA
    AAGAATGTCT CATCAGCGCA AACACCAATC ACCCAAGTCG TTAATGAAAA ACCAGCTACA
    CGAAAGAGTG AACGCATTGA TAAAGAAAAG CTAAAACGAT CCCCCAGAGG AGACTCTACA
    AAACTTACTG CTGATTCCAG AAATTCTGCA AAATCTCCTG TTCAAGTTTT AGATTCAGAG
    CAGGGCCTTG AATTAAACAC AAACCAAGGC AGAACACGCC AACGACGGAA TGTGCGATCT
    GTCTATGCAA CACCCCCAGA GGAAGAATCT CCTCAACAAC AAGGAAAAGA GGTAACAGAA
    CCTCCCCGTT CCACTCTTAA GCGCTCTAGG GGTCGTCCAC CCAAGACTCG CAGACGAGTG
    GATGATATGT CTCCGGTAAA AGGAGATCAG ATCAAAAATT CTGAGGCTGA AGACACTGAA
    AATAAAGAGT CAACAAGTAG TGGAGAAATA ACTAAAAGTA CAGAGGCATG GCGCTCTCCA
    CGTTCTCAAA AGGGCCAGTC TTCACCGATA AGATCAGGAC AAAGCAAAAA GACATTGAAA
    TTGGACAAAG TGTCAGGTAC TTCGGCATGT GTTCAATTAG ACAGAGAAGC AGTTGATGTG
    CCACCTGCCC TAGATCGCAA AGCTGAATCC AAAGAAACTT TGATGCAAGT TGGACATTTG
    TCAAAAGATA CCAAACAGAA CAGCATATCT ACAAGAGAAA AAGAACTTGT TGCTGAGGAA
    ATTTCAACAG ATGACCGTGT AGAAATCACT CCTGTAGAAA AAATATTAGA CAAGGAGGTC
    AAGACATCAA GGTCAACACG GAACACAAAA ACAATGTCTG ATAATAAGGC TGTCAATGTG
    GTGAAGCTCA CTATGGATGC AGTTAAGGAA GCTGTTCATT CAGGTGATGA CATAACTGTT
    TGTTTTGAAG GGTCTTTAAA AGCTAATGTT CCACAATCAG TAATAGAACA GTCAGACATT
    CAAGATTATC ACAAAAAGGA AAATGTTAAC AGCCTTGATG AAAAGGATGA GACTCTTTCA
    GAAATGGAGC CTCCTACTGA TCCTGTGGCT GCCCTGCTTG CTCGCCAAAT GGAACTGGAA
    AGAGCAGTGG AAAATATTTC AAGATTTACA GATGACCAGC ATCCAGTACC TTACAAAGAA
    CCTCGTACAG AACCACCTAC CCTAACAGCA CCTGTTGTAG TACAACCTGC AGAGGATACA
    GAGGTAGGAA AGCCTGCCAA TCCAGCAAGT GAAACTGAAT TGGCTGAAGC AATTAATTCA
    ATTACTGCTG AGGATATATC AGGTGATACA GATGGATTCT CTGCTTCAAC AACATACACA
    ACCCTCCTTC CTCAACCAGA GACTCTTGAT TTGCCTGTAC CAAATGAGGT TGTGGAAACT
    GAGTCAGACT TGACTGTAAA GACTATTGCA CAGTCTGAAC AAGATAATGT TATGGTTCCA
    GTTTCAAAAT CTTCTAAATG TAAAACAGAT ACTTCACTCA AAGAATCACC ATTGACAGAG
    GTAGCAAAAA GAGGAGGAAG AGCACGTCCA AAAACTGCAA AAAAATCCAG AGGGAGGAAG
    GTTTCTTTGA ACAGAAAATT AGATATTGCT GAGGATGTAG TGCTGGAACC TGAATCCACT
    ACAGTCAAAC TTCCAGAATC GATTCCAGAA GAGATTCAAA CAGTTAACCC TAAGGCAGCA
    ACATCGGCAG CTGCAGCAGC TGTAGTCACA GTTGCTGCTG CATGCAAACA TGAAGCTGCA
    TCTACAGTGA TTTTGAATAC ACCAAAAGAG GCAGAACAGC CTGCAGTTGA TCAGCCTGAA
    CCTCAAGAAT CTGCCTTCCA TTCTGGAAAT AACAGTCCTT CTTATTTGAG GACACAACAG
    TCATCTCCTG AGCGAGGAGC ATCTGCCCTT ACTTCACCTA CAACACGCCT AAATTCACCT
    GCATCCTCTA ACATTCCTCC TGAATGGAAT ACCAGGACAG AGGAGAAAGT AATGCTACCT
    AAACCACAAG GTAATGTGGC TTTGTCTATC CCAGGAGCTG GGGGACCCCC AGCAAATCCT
    CCCATGCCTC CTGACACAAA AGCCTCTGAT ATTAATGCAA GTTCTAGTAC ACTGCGAAAA
    ATTTTAATGG AACCAAAGTA TGTTTCTGCT TCAAATAGCA ATTCAGTGAC AAGTATGCAG
    TTTACTACCA CATTAGCAGA TCCACGGATG TCTGATAATG AAAATTCAGT TGAGGCTGTG
    CTGCCTTTAA AAACCTCTCT ACCTGAAGAT AGACCTAGCC CTATAGCTCA GCTTGTACCC
    CGTACAACAC CACCGCAGCC CCCTCCTCTA CAACAATGTG AGACTCCACA GATCTTAAAG
    GAGAAACTAG CTATAACTTC CACTGCCACT TCAGTCATTA GTCGGATCCC TATGCCCTTT
    GATTTTGAGG ACACTCCTCG CATCTCTTTA AGTAATCGTA ACTCTGGAAT GTCTTTGCCA
    AAGCAGAAAT ATCGTACTGG CTTGAATGAA AATAATAGAT ATCATGGACA TAATACTTCT
    GAGGATGGTG GAAGTGTTGG GCGCCCAGTT GTTGAAGGCA CACATTGTAA CACGGGGTCT
    AGTACTGGTT TGAGGGTCAA CACATCTGAG GGTGTGGTAG TATTAAGTTA TTCTGGACAA
    AAGACAGAGG GACCACAGCG GATCATTGCA AAAATTAGCC AAATCCCTCC AGCCAGTGCA
    GTTGACATTG AATTTCAGCA GTCTATAACA AAATCTCAGA TTAAACAAGA ACCTTCATCT
    CACCCCTCTA CCCCAAAAGG ATCACAGACA CCTACAGGCT ATAGTCACAC AGGGGTAGTA
    TTAGCTGGCC AAAGTATCAA TGCCCAACCT GTTATATCCT CAATACGCCA GGAAAGCCCT
    GGCTCTGAAA AATCAGAAAC ATCTTACCAA CAAGGAAGTT CTCTGAAATC TTTCCACCAG
    TCCCCAAGCC ATCCTCAACT TCTGAGGTAT GGTCAGTCAA TAACACCGCA ACACCATATG
    AAGAAAAATG GAGAAACTGA ATCATTTTCA ATGAAAGCTG ATATAAAACC TGATATAAAG
    GCAACACAGA CTTTCAATGT TAAACCAGTT TTAAGTCCAC GCCACCCATC TTTCTCAGGC
    AATCACATTT TAAGTCCAAG TGCTCCACAT GAAAGAGCTG TCCCACAACC CAAGCAAGAT
    TCACATTCTC CAAGGGCATC TAACCATTCC ATGTCACCCT TTCCAAAGGT TTGCCCCCCT
    AACAGCCCCG TTGTTCTGGG ACCAGCTGGC CCTATGTCTC AGTATGTGTC AAATATACAC
    CATGCTGAGC AGTCAGTGAT CATGCCTCCT CATAGTGTCA CCCAGTCTGT GCCCATGAGT
    CATTTGTCCC AAGGGGATGT CCGAGCCAGT ACTCCAACAT TATCTGGAAT AGGTTATGGA
    TTGCGTTCAG AAAATCTCTT ATCACCTCGA TCGGCACCTC CTCAACGTTC CACGACTCCT
    CAACCTGCAG TGATCAGAGA TGTCATATTG AAATCGCATG CAGGCTCGTC TGTTGGTGGC
    CATGTTGTTG AGACGAGCAA TGATGATGCA CAGAATCTCC CACAAGGACT TCGTAGATCT
    TCTGCCCCAC AGTTGCAACA GGAAAGTATG GTAATACAGT CGGAATTCAA AAATCTGCAT
    CACAGGGCAT TACGTCTAGA TCAGTATGCC AGACTTATGC AGCAGCATTT GACTGACCAT
    CCAGGTGTTG TTGAAAGTCG CCAAACTCGA ACTGCAGAGG TGGTGCAGTC ATCTTCACAC
    ATATCATCTG CTTCTTCTAA AGCCTCTTCC ATAGTAAAGA ATGCTCCACA GATTGTAAGG
    GATGGACAAA AAACAGTGGA GCTGAAGATG TCACCCTCCC CTCATTCAGA CAACAGGATA
    ACAGGACACC CACCAGGCTC AGTGATGGTA TCTCCTCAAG GAGTTCAGTT AAATTACCCT
    GGAAATGGAA ATACCATGAA TGAATACTAC AAAGAAATGC GTGGCTTCCA TCCTCAGTAT
    CCAGGTCATT CAGTAATTGG AATTAACTTG GCTAACCGTG GGATTCCTGT GTCTCAGGTC
    TCACAAATTG ATCACAGTCA AAGACACAAG GTTCCCTCTC TCTCTTCCAG TGAGTCTGTG
    GGAAGTTTTA GTGAGTCAAA ACTTGAGGGT TCTCACATTC GACACTCAGG CACCATGGAT
    TTGTCTCATA TATCACGAGT TCAGAGTGAG GCTGGGTCCC CCTCTTATAT TTCTCCAGTG
    ACTATAACAC CCAAACTGGA GTTGGCTATT ACTTTGCAGA AGGGGCCTCA AGGACCTGTT
    TCCAACAAGA TGCCACTTCC TTCTTTAGCT TCTTCACAGA TGCGTTCAGA CTTCAAACTT
    GACCACACAG GACTTCGGTC TGTTGATATG GTGCAGTTGT TAACGAAGTA CCCAATCATT
    TGGCAAGGTC ACCTGGCACT TAAGAATGAC ACTGCAGCAG TGCAGCTGCA CTTTGTGTCT
    GGTAATAATG TTCTGGCTCA TCGTTCACTG CCACCCCCAG AAGGAGGTGC CTTTCTTCGC
    ATTGCTCAGA GAATGCGCTT AGAGGCTTCA CAACTGGAAG GAGTTGCGAG ACGCATGACT
    GCTGAAAATG AATACTGCCT TCTGTTGGCA TTGCCTTGTG GATTGGACCA AGAAGATGTC
    CACAACCAGA CCCATGCTCT TAAAACTGGT TTTATTACTT ACCTGCAAGC AAAACAGGCT
    GCTGGAATTA TCAATGTCCC AAACCCAGGC TCAAATCAGC CTGCCTACGT TGTCCAGATT
    TTCCCTCCTT GTGAGTTTTC TGAGAGCCAC CTGTCTCATC TTGCTCCGGA CCTTCTCAAC
    AGCATCTCCA GTATCTCTCC TCATCTTATG ATTGTTATAG CTTCTGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27765
    Clone ID Related Accession (Same CDS sequence) XM_003201204.5 , XM_003201204.4
    Accession Version XM_003201204.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product msx2-interacting protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003201204.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9781
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    10201
    10261
    10321
    10381
    ATGGTCCGGG AAACCAGACA TTTATGGGTG GGAAATTTAC CAGAAAATGT ACGAGAGGAA 
    AAAATTATCG AGCACTTCAA ACGGTATGGA CGAGTGGAGA GTGTCAAGGT TCTCCCTAAA
    CGTGGATCGG AAGGTGGAGT GGCAGCTTTT GTGGATTTTG TGGACATTAA GAGTGCTCAG
    AAAGCTCATA ATTCTATCAA CAAGATGGGA GACAGGGACC TTCGCACCGA TTACAATGAA
    CCAGGAACTA TACCCAGTGC AGCTCGAGGC CTGGATGATA GCCTGTCCAT AGCCACTCGT
    GGGCGGGATG TTTCAGGGTT CACAAGGGGG GCAGGGGGCC CTGTGTATGG GCCCTCTGTG
    TCACTTCACA GCAGAGAGGG ACGCTTTGAA CGCAGACTAG ACGGGACTTC GGACAGCCGG
    GAACGCTCTT ACGATCACAG TGCCTATGGA CACCATGAGC GTAGCAGCAG CAACAGCACT
    AGTTTCGATC GGCAGCGTCA CTATGAGAGC GATTATTACA GAGACTCTAG GGACCGGGCC
    CTCAGTGGAA CCAGTGGGAG TGCCAGTTCA GCCAGTGGAA GCATCGGAGG AAGCTCATCT
    GCGGGCAGTA TAGGTGTTGC AGGGAATGTT GCAGGAAGCA GTGGTGCTGG TGGAAGTACT
    GCCGCTGGAG TAGGCAGTGG TGGCTCCACA CCAGGAGGAA TTGTCTATTA TGGTTCACGA
    AGCCGGAGCC CCAGTCGTTT TGAGACTACA GAGACTCGTT ATGAGCCCCG TGCCCGTGAA
    GCTTTCACAC TTGCCAGTGT GGTTCATCGG GATTTATTCA GAGAAGAAAG AGGCAGACGT
    GGGGAAAGGA CATACCACCA CAGTCGCAGT AGATCACCAC ACTCTTCACA GTCACACAAT
    CCATCACCAC AGAGGTTGCA AAGTCAGGCA GCGCGACCGG CACGCTCCCG CAGTGGCTCA
    GGATCCCGCA GTCGCTCCTC CAGCTCAGAC TCCGTCAGCA GTACCAGCAG CAGTGGCAGT
    GGCAGTAGTG ATTCCAGCAG CACCTCCAGT GACCGATCTC CAGCTCGATC TGTTCAGTCC
    ACTGCAGTTC CTGCCCCTTC TACACAGCCA CTACCTTCAC TGGACAAAGA TGAACCTCGT
    AAAAGTTTTG GTATCAAAGT GCAGAACCTG CCCGTGCGCT CAACAGATAC AAGCCTCAAG
    GATGGTCTTT TCCATGAATT TAAGAAACAT GGTAAAGTCA CATCAGTTCA GATCCACGGT
    GCCTCTGAGG AACGATATGG CTTAGTGTTC TTTCGCCAGC AAGAGGACCA AGAAAAAGCT
    CTAAGTGCAT CTAAAGGGAA GCTGTTTTTT GGCATGCAAA TTGATGTCAC TGCTTGGCAT
    GGCCCAGAAA CAGAAAGTGA GAACGAGTTC CGGCCTTTGG ATGAACGCAT AGATGAGTTT
    CATCCCAAAG CTACAAGGAC ATTGTTCATA GGAAATTTGG AAAAAACCAC AACCTATAAT
    GACCTGCTGA ACATATTTCA GCGTTTTGGA GAGATTGTTG ATATTGATAT TAAAAAGGTA
    AATGGTTCCC CACAATATGC CTTTCTCCAG TATTGTGACA TAGCAAGTGT CTGCAAAGCA
    ATTAAGAAAA TGGATGGTGA ATATCTTGGC AACAACAGGT TAAAGCTTGG TTTTGGCAAG
    AGTATGCCTA CTACATGTGT ATGGTTGGAT GGCTTGTCCT CCAGTATTAC TGAACAGTAT
    CTCACGAGGC ACTTTTGTCG TTATGGACAT GTAGTAAAGG TTGTCTTTGA CCGGCTGAAA
    GGAATGGCCC TCATCTTATA TAATAATATA GAGTATGCGC AGGCTGCTGT CAAGGAAACA
    AAGGGTTGGA AAATTGGAGG AAATAAAATA AAGGTTGACT TTGCTAACCA GGAAAGTCAG
    ATGGCTTTCT ACCGTTCAAT GCAGGGATCT GGACAAGACA TAAGAGATTT CTATGAAATC
    ATATCTGAAC GAAGAGATGA GCGCCGGACG CCATATCACG AGTTCACAGC TGAACGTGCA
    TACTATGAAA ATGTGCGTAC CCCAACTTCT TACACAGAGG ATCCACGTCG AAAATATCCT
    GCCAGAAGTC GAGAGTTTTT TTCAGAATGG GATCCCTACC AGGGAGATTA CTATGACCCA
    CGATATTATG ATGACTCCCG TGAATACAGG GATTTTAGAG ACCCATATGA GCAGGACATT
    AGAAAGTACA GCTACTTGCA AAGAGAACGT GAAAGGGAAA GAGAGAGGTT TGAAACAGAT
    CGTGAGCGAG ACCATGGTCG ACGAACAATT GAACACCAAC AAAGCCCAAC CCATTCACGT
    CGTCCTGCTA GTCCAACTGC TTCTCCATCC CTTTCTGAAC GTCCTCCCAG TGACTCTGAA
    CATCACGTTT ACAGTCGCTC ATCTGAGCGA AGTGGTAGCT GCAGCTCACT TTCTCCACCA
    CGCTTTGAGA AGCCAGACAA AATTCGTTTG GAGAGGCACA ATAGGAATGA TAAATTGGAG
    AAAGAAAAAT CACTTTTTGA GACAGAGCGT GGAAATGGTG GGGAAAAAGA AAGGCGAGCA
    GGACGTAAGG AAAAAGGAGA AAAGGACAGG ACTGAGAGAC AGAAGTTGAA GAAGTTAAAA
    CTTGCATCTC CCACTGTTCC ATCACCAGAG GCAGAGCTGG AACTTGACAG GGATGCAAGC
    CCAGAGGCTA ACATAACTCT GCGAGGTAAA GTGAGCAAAT CATTGATCAA AGACAAAGAC
    TACTCTGGTA AAGGAAAACT TGATCTGCTA CCTTGTGTTG TCCAGCTAAC AAGAGTAAAA
    GAGAAGGAAG GAAAATTAAT AGACAGTATC CTCTGTGAAA AACAAAAAAC AAAGGTTGGA
    AGTGATCCTG TTCTGTCTCC AACAACACCT TCCTCAGGTG ATCACAAGAG TATGCCTTTT
    CGCAAAGACA CTCAGGCTAG AGATTTCTTT AAGCATGGAA AACATCTCAA GGAGAAAGGC
    CTGGCAAGCC AAGTTGAGGT AGTGGATAAA GAGGAAAAAG TGAAAAACAA AAAATATTTC
    AAATCTGATC TTGCATTTGA ATCCAGCTCT TCTGTGGATG CTGACCGCAA GGCTGCACGA
    AAGAGACGCT TTGAAGAAAC ATCGGCTAAG GCTGATAATT TAAGGAGAGT AAGTCAGGAG
    GAGGATGAAG GGAAATTAAG GAGAATTATT GATGAACCTT TGCTGAAAGA CACTGATTAT
    GATAAAAAGT TGCTTCGGAA AGAAGCACAC AAGAGAGAGC GAAAAATTAA ACCAGAGAGG
    ATGGTTACTG TTAGTACTAC AATAGAAGAG TTAGACAATG TAATGCCAGT AGGCCCCAGT
    TTAGACCTTC AGGCTCGACT TGGGGAACCA GCTGAAGATG CAATAGATCC ATTAGATAGC
    CTTGATCAAA AGATAGGTGC TTTTGGAGCT AACAGACAAC CAAGTTTCAG TCTTGCAGTC
    TCCGACGATG GAAGTGTAGA TATGGATTTT TCAAGAGAGC AAGAGCAGCA GCATTTAACT
    AGCTACCACA CATTATCCTC AAGGCAAGAA AGAGTTTCTG AAAGTAAGGA AAGTTTGCTC
    TCAGACATTG ATCATTCGCA GAGTTGCAGA AAACAAATGG AGCAAAATCG CCGCTTACAG
    CAGCAAATGC TGGAGTGTGA TAAATCAGAT AAAACTGAAA GCACACCAAG CACTGATGCG
    GAGGAATTTG AGCATCGAAG TATTGTGCAT GAGGTTGTAA AACCATTACA AGATGTAACG
    GGAAGTTCTC CGACCAGCAA GCAAAAAAAA TTGGGAGGAT TCGAATTTGA TTTTGGCAAA
    AGAGAGCAAA ATTATGAAAT GTTTCGACTG AGAAATGATG AACCTGAAAG AGGACTTGCT
    TCACATCCAG GGACACCCCT TGCTGAGGAG GAGAGAAATG CATCACAATT GTTGGACAAA
    GACCCAGATT TACCAGTGAC AATGGATAAA AATAGCTCGC ATTTAGATGT TTCAAAATAC
    AATACTAGCA ATGCAGTGCA TCAAGGGCTT TCACCACATG CAGAGATACT AAAAGTGAAA
    CCATCAGTTA CTAAAGAGGA GTTCAGTTGG GAGAGCAATA TTAGGCAGGG CACATTGAGA
    GAAATGAGCT TTCCCACAAG CATTGTTAAA CGGGAAAGTA TCAGAAAACG TCCTGAACGC
    GAATTAGAAC CTGGAGAGGT TCAGTCAGAC TCTGATGAAG ATGAAAGCAG ACACTATTCA
    TTAAAGCCAA TTTCTTTCAA AAGAGAGCAT GAAGAGCGGC TTTCAGATGT AAAGTATTCT
    GAGTCTCTTG AAAAGAATAA GTTCTATGAA TTTGCATTGG ATAAGACGAT AACACCAGAC
    ACAAAAGCTT TGCTTGAGCG TGCTAAGTCA CTGTCTTCAT CTAGAGAAGA AAATTGGTTT
    TTCCTTAATC ATGACTCAAA ATTCAAAAGT TTACAGAATA ACATCGACAA GGAAAAGTCT
    GAACCTACTC CAAGACCTAT TCCTTCATGG TACTTGAAGA AAAAGAAAAG TCGCTCTGAC
    TCTGATGGAA AACTTGATGA AAAAAAGCAA GATGCTAAGC CTGATGAACA AGAAAGACAA
    GATCTCCTAG CTTCACGATT TCTTCATAGC TCAATCTTTG AACAGGATTC ACGTCGGCTT
    CAACACCTTG AACGGAAAAA TACTGACCCT GACGTTAGAA TTGGCAGAGA TAGTGTTACA
    AGTAATTCTC AAGGTGAACA ACCTGGAACA GGAGGATCAG ACTTGACTCA AGAACCAAGA
    GTACTTTTTC ATAGCCGCTT TTTGGAACTT CAGCAAAGAG ACAAGAACCA ACAGCTTCCT
    ATTTCAGAAA AACTTAGTTT AACTGATCAA ATGGAGAAGA CATTGGATGA AGAGTTTAGG
    CCATCTCCCA ATTTATCAGA CATTTCCCAA GAGTCATTAG CAAGTCATAG CTTAACACCT
    ACAATATCCC CAGTGTCTCA GTCTAAAATT TCAGAGATGT CTGTGCACCA TGAGAAGCCT
    GTTTTGCATA CTACTTTACC AAACATACAT AATCCAGCAG TTGTGAATGT TAGGTCAGAA
    AATGCTCCAA TAGATTATCC ATCGTTAATT AGCCTAAAGG ATGAGAACAA CACTATTCCG
    TTGAGCACTC CATCACCTGA GCCCATGATA AAGGAAGATG TAATTGTTGA AGAACCTGAA
    GAAAAACTCA GAGATTCCAG AAAAACTGTA CACGCCGCTG TTGAACACGA GATTGAGATC
    AAACCTCCCA CTCCTGGTGC ATCTTTAGGT AATGTTGAGC CAGAATGTGA TGCTCTGCAG
    GCATCTTCTC CATTGTATCC AAATCCCAGT AAAGATCAAG AGACTACAGA GCTCTCAGAA
    ACAACAACAG AGCCTGCTAA TTCTTCTCTT GTTGTATCTC CTCTGGAGGT GCCAGACACA
    GAAATTGAGC AAGTTGAACA AGTACTACCA CCGCGTAAAC AACCAAAGAG TAAAAAGGTA
    AAGAATGTCT CATCAGCGCA AACACCAATC ACCCAAGTCG TTAATGAAAA ACCAGCTACA
    CGAAAGAGTG AACGCATTGA TAAAGAAAAG CTAAAACGAT CCCCCAGAGG AGACTCTACA
    AAACTTACTG CTGATTCCAG AAATTCTGCA AAATCTCCTG TTCAAGTTTT AGATTCAGAG
    CAGGGCCTTG AATTAAACAC AAACCAAGGC AGAACACGCC AACGACGGAA TGTGCGATCT
    GTCTATGCAA CACCCCCAGA GGAAGAATCT CCTCAACAAC AAGGAAAAGA GGTAACAGAA
    CCTCCCCGTT CCACTCTTAA GCGCTCTAGG GGTCGTCCAC CCAAGACTCG CAGACGAGTG
    GATGATATGT CTCCGGTAAA AGGAGATCAG ATCAAAAATT CTGAGGCTGA AGACACTGAA
    AATAAAGAGT CAACAAGTAG TGGAGAAATA ACTAAAAGTA CAGAGGCATG GCGCTCTCCA
    CGTTCTCAAA AGGGCCAGTC TTCACCGATA AGATCAGGAC AAAGCAAAAA GACATTGAAA
    TTGGACAAAG TGTCAGGTAC TTCGGCATGT GTTCAATTAG ACAGAGAAGC AGTTGATGTG
    CCACCTGCCC TAGATCGCAA AGCTGAATCC AAAGAAACTT TGATGCAAGT TGGACATTTG
    TCAAAAGATA CCAAACAGAA CAGCATATCT ACAAGAGAAA AAGAACTTGT TGCTGAGGAA
    ATTTCAACAG ATGACCGTGT AGAAATCACT CCTGTAGAAA AAATATTAGA CAAGGAGGTC
    AAGACATCAA GGTCAACACG GAACACAAAA ACAATGTCTG ATAATAAGGC TGTCAATGTG
    GTGAAGCTCA CTATGGATGC AGTTAAGGAA GCTGTTCATT CAGGTGATGA CATAACTGTT
    TGTTTTGAAG GGTCTTTAAA AGCTAATGTT CCACAATCAG TAATAGAACA GTCAGACATT
    CAAGATTATC ACAAAAAGGA AAATGTTAAC AGCCTTGATG AAAAGGATGA GACTCTTTCA
    GAAATGGAGC CTCCTACTGA TCCTGTGGCT GCCCTGCTTG CTCGCCAAAT GGAACTGGAA
    AGAGCAGTGG AAAATATTTC AAGATTTACA GATGACCAGC ATCCAGTACC TTACAAAGAA
    CCTCGTACAG AACCACCTAC CCTAACAGCA CCTGTTGTAG TACAACCTGC AGAGGATACA
    GAGGTAGGAA AGCCTGCCAA TCCAGCAAGT GAAACTGAAT TGGCTGAAGC AATTAATTCA
    ATTACTGCTG AGGATATATC AGGTGATACA GATGGATTCT CTGCTTCAAC AACATACACA
    ACCCTCCTTC CTCAACCAGA GACTCTTGAT TTGCCTGTAC CAAATGAGGT TGTGGAAACT
    GAGTCAGACT TGACTGTAAA GACTATTGCA CAGTCTGAAC AAGATAATGT TATGGTTCCA
    GTTTCAAAAT CTTCTAAATG TAAAACAGAT ACTTCACTCA AAGAATCACC ATTGACAGAG
    GTAGCAAAAA GAGGAGGAAG AGCACGTCCA AAAACTGCAA AAAAATCCAG AGGGAGGAAG
    GTTTCTTTGA ACAGAAAATT AGATATTGCT GAGGATGTAG TGCTGGAACC TGAATCCACT
    ACAGTCAAAC TTCCAGAATC GATTCCAGAA GAGATTCAAA CAGTTAACCC TAAGGCAGCA
    ACATCGGCAG CTGCAGCAGC TGTAGTCACA GTTGCTGCTG CATGCAAACA TGAAGCTGCA
    TCTACAGTGA TTTTGAATAC ACCAAAAGAG GCAGAACAGC CTGCAGTTGA TCAGCCTGAA
    CCTCAAGAAT CTGCCTTCCA TTCTGGAAAT AACAGTCCTT CTTATTTGAG GACACAACAG
    TCATCTCCTG AGCGAGGAGC ATCTGCCCTT ACTTCACCTA CAACACGCCT AAATTCACCT
    GCATCCTCTA ACATTCCTCC TGAATGGAAT ACCAGGACAG AGGAGAAAGT AATGCTACCT
    AAACCACAAG GTAATGTGGC TTTGTCTATC CCAGGAGCTG GGGGACCCCC AGCAAATCCT
    CCCATGCCTC CTGACACAAA AGCCTCTGAT ATTAATGCAA GTTCTAGTAC ACTGCGAAAA
    ATTTTAATGG AACCAAAGTA TGTTTCTGCT TCAAATAGCA ATTCAGTGAC AAGTATGCAG
    TTTACTACCA CATTAGCAGA TCCACGGATG TCTGATAATG AAAATTCAGT TGAGGCTGTG
    CTGCCTTTAA AAACCTCTCT ACCTGAAGAT AGACCTAGCC CTATAGCTCA GCTTGTACCC
    CGTACAACAC CACCGCAGCC CCCTCCTCTA CAACAATGTG AGACTCCACA GATCTTAAAG
    GAGAAACTAG CTATAACTTC CACTGCCACT TCAGTCATTA GTCGGATCCC TATGCCCTTT
    GATTTTGAGG ACACTCCTCG CATCTCTTTA AGTAATCGTA ACTCTGGAAT GTCTTTGCCA
    AAGCAGAAAT ATCGTACTGG CTTGAATGAA AATAATAGAT ATCATGGACA TAATACTTCT
    GAGGATGGTG GAAGTGTTGG GCGCCCAGTT GTTGAAGGCA CACATTGTAA CACGGGGTCT
    AGTACTGGTT TGAGGGTCAA CACATCTGAG GGTGTGGTAG TATTAAGTTA TTCTGGACAA
    AAGACAGAGG GACCACAGCG GATCATTGCA AAAATTAGCC AAATCCCTCC AGCCAGTGCA
    GTTGACATTG AATTTCAGCA GTCTATAACA AAATCTCAGA TTAAACAAGA ACCTTCATCT
    CACCCCTCTA CCCCAAAAGG ATCACAGACA CCTACAGGCT ATAGTCACAC AGGGGTAGTA
    TTAGCTGGCC AAAGTATCAA TGCCCAACCT GTTATATCCT CAATACGCCA GGAAAGCCCT
    GGCTCTGAAA AATCAGAAAC ATCTTACCAA CAAGGAAGTT CTCTGAAATC TTTCCACCAG
    TCCCCAAGCC ATCCTCAACT TCTGAGGTAT GGTCAGTCAA TAACACCGCA ACACCATATG
    AAGAAAAATG GAGAAACTGA ATCATTTTCA ATGAAAGCTG ATATAAAACC TGATATAAAG
    GCAACACAGA CTTTCAATGT TAAACCAGTT TTAAGTCCAC GCCACCCATC TTTCTCAGGC
    AATCACATTT TAAGTCCAAG TGCTCCACAT GAAAGAGCTG TCCCACAACC CAAGCAAGAT
    TCACATTCTC CAAGGGCATC TAACCATTCC ATGTCACCCT TTCCAAAGGT TTGCCCCCCT
    AACAGCCCCG TTGTTCTGGG ACCAGCTGGC CCTATGTCTC AGTATGTGTC AAATATACAC
    CATGCTGAGC AGTCAGTGAT CATGCCTCCT CATAGTGTCA CCCAGTCTGT GCCCATGAGT
    CATTTGTCCC AAGGGGATGT CCGAGCCAGT ACTCCAACAT TATCTGGAAT AGGTTATGGA
    TTGCGTTCAG AAAATCTCTT ATCACCTCGA TCGGCACCTC CTCAACGTTC CACGACTCCT
    CAACCTGCAG TGATCAGAGA TGTCATATTG AAATCGCATG CAGGCTCGTC TGTTGGTGGC
    CATGTTGTTG AGACGAGCAA TGATGATGCA CAGAATCTCC CACAAGGACT TCGTAGATCT
    TCTGCCCCAC AGTTGCAACA GGAAAGTATG GTAATACAGT CGGAATTCAA AAATCTGCAT
    CACAGGGCAT TACGTCTAGA TCAGTATGCC AGACTTATGC AGCAGCATTT GACTGACCAT
    CCAGGTGTTG TTGAAAGTCG CCAAACTCGA ACTGCAGAGG TGGTGCAGTC ATCTTCACAC
    ATATCATCTG CTTCTTCTAA AGCCTCTTCC ATAGTAAAGA ATGCTCCACA GATTGTAAGG
    GATGGACAAA AAACAGTGGA GCTGAAGATG TCACCCTCCC CTCATTCAGA CAACAGGATA
    ACAGGACACC CACCAGGCTC AGTGATGGTA TCTCCTCAAG GAGTTCAGTT AAATTACCCT
    GGAAATGGAA ATACCATGAA TGAATACTAC AAAGAAATGC GTGGCTTCCA TCCTCAGTAT
    CCAGGTCATT CAGTAATTGG AATTAACTTG GCTAACCGTG GGATTCCTGT GTCTCAGGTC
    TCACAAATTG ATCACAGTCA AAGACACAAG GTTCCCTCTC TCTCTTCCAG TGAGTCTGTG
    GGAAGTTTTA GTGAGTCAAA ACTTGAGGGT TCTCACATTC GACACTCAGG CACCATGGAT
    TTGTCTCATA TATCACGAGT TCAGAGTGAG GCTGGGTCCC CCTCTTATAT TTCTCCAGTG
    ACTATAACAC CCAAACTGGA GTTGGCTATT ACTTTGCAGA AGGGGCCTCA AGGACCTGTT
    TCCAACAAGA TGCCACTTCC TTCTTTAGCT TCTTCACAGA TGCGTTCAGA CTTCAAACTT
    GACCACACAG GACTTCGGTC TGTTGATATG GTGCAGTTGT TAACGAAGTA CCCAATCATT
    TGGCAAGGTC ACCTGGCACT TAAGAATGAC ACTGCAGCAG TGCAGCTGCA CTTTGTGTCT
    GGTAATAATG TTCTGGCTCA TCGTTCACTG CCACCCCCAG AAGGAGGTGC CTTTCTTCGC
    ATTGCTCAGA GAATGCGCTT AGAGGCTTCA CAACTGGAAG GAGTTGCGAG ACGCATGACT
    GCTGAAAATG AATACTGCCT TCTGTTGGCA TTGCCTTGTG GATTGGACCA AGAAGATGTC
    CACAACCAGA CCCATGCTCT TAAAACTGGT TTTATTACTT ACCTGCAAGC AAAACAGGCT
    GCTGGAATTA TCAATGTCCC AAACCCAGGC TCAAATCAGC CTGCCTACGT TGTCCAGATT
    TTCCCTCCTT GTGAGTTTTC TGAGAGCCAC CTGTCTCATC TTGCTCCGGA CCTTCTCAAC
    AGCATCTCCA GTATCTCTCC TCATCTTATG ATTGTTATAG CTTCTGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003201252.2
    CDS2251..12681
    Translation

    Target ORF information:

    RefSeq Version XM_003201204.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spen family transcriptional repressor (spen), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003201204.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    ATGGTCCGGG AAACCAGACA TTTATGGGTG GGAAATTTAC CAGAAAATGT ACGAGAGGAA 
    AAAATTATCG AGCACTTCAA ACGGTATGGA CGAGTGGAGA GTGTCAAGGT TCTCCCTAAA
    CGTGGATCGG AAGGTGGAGT GGCAGCTTTT GTGGATTTTG TGGACATTAA GAGTGCTCAG
    AAAGCTCATA ATTCTATCAA CAAGATGGGA GACAGGGACC TTCGCACCGA TTACAATGAA
    CCAGGAACTA TACCCAGTGC AGCTCGAGGC CTGGATGATA GCCTGTCCAT AGCCACTCGT
    GGGCGGGATG TTTCAGGGTT CACAAGGGGG GCAGGGGGCC CTGTGTATGG GCCCTCTGTG
    TCACTTCACA GCAGAGAGGG ACGCTTTGAA CGCAGACTAG ACGGGACTTC GGACAGCCGG
    GAACGCTCTT ACGATCACAG TGCCTATGGA CACCATGAGC GTAGCAGCAG CAACAGCACT
    AGTTTCGATC GGCAGCGTCA CTATGAGAGC GATTATTACA GAGACTCTAG GGACCGGGCC
    CTCAGTGGAA CCAGTGGGAG TGCCAGTTCA GCCAGTGGAA GCATCGGAGG AAGCTCATCT
    GCGGGCAGTA TAGGTGTTGC AGGGAATGTT GCAGGAAGCA GTGGTGCTGG TGGAAGTACT
    GCCGCTGGAG TAGGCAGTGG TGGCTCCACA CCAGGAGGAA TTGTCTATTA TGGTTCACGA
    AGCCGGAGCC CCAGTCGTTT TGAGACTACA GAGACTCGTT ATGAGCCCCG TGCCCGTGAA
    GCTTTCACAC TTGCCAGTGT GGTTCATCGG GATTTATTCA GAGAAGAAAG AGGCAGACGT
    GGGGAAAGGA CATACCACCA CAGTCGCAGT AGATCACCAC ACTCTTCACA GTCACACAAT
    CCATCACCAC AGAGGTTGCA AAGTCAGGCA GCGCGACCGG CACGCTCCCG CAGTGGCTCA
    GGATCCCGCA GTCGCTCCTC CAGCTCAGAC TCCGTCAGCA GTACCAGCAG CAGTGGCAGT
    GGCAGTAGTG ATTCCAGCAG CACCTCCAGT GACCGATCTC CAGCTCGATC TGTTCAGTCC
    ACTGCAGTTC CTGCCCCTTC TACACAGCCA CTACCTTCAC TGGACAAAGA TGAACCTCGT
    AAAAGTTTTG GTATCAAAGT GCAGAACCTG CCCGTGCGCT CAACAGATAC AAGCCTCAAG
    GATGGTCTTT TCCATGAATT TAAGAAACAT GGTAAAGTCA CATCAGTTCA GATCCACGGT
    GCCTCTGAGG AACGATATGG CTTAGTGTTC TTTCGCCAGC AAGAGGACCA AGAAAAAGCT
    CTAAGTGCAT CTAAAGGGAA GCTGTTTTTT GGCATGCAAA TTGATGTCAC TGCTTGGCAT
    GGCCCAGAAA CAGAAAGTGA GAACGAGTTC CGGCCTTTGG ATGAACGCAT AGATGAGTTT
    CATCCCAAAG CTACAAGGAC ATTGTTCATA GGAAATTTGG AAAAAACCAC AACCTATAAT
    GACCTGCTGA ACATATTTCA GCGTTTTGGA GAGATTGTTG ATATTGATAT TAAAAAGGTA
    AATGGTTCCC CACAATATGC CTTTCTCCAG TATTGTGACA TAGCAAGTGT CTGCAAAGCA
    ATTAAGAAAA TGGATGGTGA ATATCTTGGC AACAACAGGT TAAAGCTTGG TTTTGGCAAG
    AGTATGCCTA CTACATGTGT ATGGTTGGAT GGCTTGTCCT CCAGTATTAC TGAACAGTAT
    CTCACGAGGC ACTTTTGTCG TTATGGACAT GTAGTAAAGG TTGTCTTTGA CCGGCTGAAA
    GGAATGGCCC TCATCTTATA TAATAATATA GAGTATGCGC AGGCTGCTGT CAAGGAAACA
    AAGGGTTGGA AAATTGGAGG AAATAAAATA AAGGTTGACT TTGCTAACCA GGAAAGTCAG
    ATGGCTTTCT ACCGTTCAAT GCAGGGATCT GGACAAGACA TAAGAGATTT CTATGAAATC
    ATATCTGAAC GAAGAGATGA GCGCCGGACG CCATATCACG AGTTCACAGC TGAACGTGCA
    TACTATGAAA ATGTGCGTAC CCCAACTTCT TACACAGAGG ATCCACGTCG AAAATATCCT
    GCCAGAAGTC GAGAGTTTTT TTCAGAATGG GATCCCTACC AGGGAGATTA CTATGACCCA
    CGATATTATG ATGACTCCCG TGAATACAGG GATTTTAGAG ACCCATATGA GCAGGACATT
    AGAAAGTACA GCTACTTGCA AAGAGAACGT GAAAGGGAAA GAGAGAGGTT TGAAACAGAT
    CGTGAGCGAG ACCATGGTCG ACGAACAATT GAACACCAAC AAAGCCCAAC CCATTCACGT
    CGTCCTGCTA GTCCAACTGC TTCTCCATCC CTTTCTGAAC GTCCTCCCAG TGACTCTGAA
    CATCACGTTT ACAGTCGCTC ATCTGAGCGA AGTGGTAGCT GCAGCTCACT TTCTCCACCA
    CGCTTTGAGA AGCCAGACAA AATTCGTTTG GAGAGGCACA ATAGGAATGA TAAATTGGAG
    AAAGAAAAAT CACTTTTTGA GACAGAGCGT GGAAATGGTG GGGAAAAAGA AAGGCGAGCA
    GGACGTAAGG AAAAAGGAGA AAAGGACAGG ACTGAGAGAC AGAAGTTGAA GAAGTTAAAA
    CTTGCATCTC CCACTGTTCC ATCACCAGAG GCAGAGCTGG AACTTGACAG GGATGCAAGC
    CCAGAGGCTA ACATAACTCT GCGAGGTAAA GTGAGCAAAT CATTGATCAA AGACAAAGAC
    TACTCTGGTA AAGGAAAACT TGATCTGCTA CCTTGTGTTG TCCAGCTAAC AAGAGTAAAA
    GAGAAGGAAG GAAAATTAAT AGACAGTATC CTCTGTGAAA AACAAAAAAC AAAGGTTGGA
    AGTGATCCTG TTCTGTCTCC AACAACACCT TCCTCAGGTG ATCACAAGAG TATGCCTTTT
    CGCAAAGACA CTCAGGCTAG AGATTTCTTT AAGCATGGAA AACATCTCAA GGAGAAAGGC
    CTGGCAAGCC AAGTTGAGGT AGTGGATAAA GAGGAAAAAG TGAAAAACAA AAAATATTTC
    AAATCTGATC TTGCATTTGA ATCCAGCTCT TCTGTGGATG CTGACCGCAA GGCTGCACGA
    AAGAGACGCT TTGAAGAAAC ATCGGCTAAG GCTGATAATT TAAGGAGAGT AAGTCAGGAG
    GAGGATGAAG GGAAATTAAG GAGAATTATT GATGAACCTT TGCTGAAAGA CACTGATTAT
    GATAAAAAGT TGCTTCGGAA AGAAGCACAC AAGAGAGAGC GAAAAATTAA ACCAGAGAGG
    ATGGTTACTG TTAGTACTAC AATAGAAGAG TTAGACAATG TAATGCCAGT AGGCCCCAGT
    TTAGACCTTC AGGCTCGACT TGGGGAACCA GCTGAAGATG CAATAGATCC ATTAGATAGC
    CTTGATCAAA AGATAGGTGC TTTTGGAGCT AACAGACAAC CAAGTTTCAG TCTTGCAGTC
    TCCGACGATG GAAGTGTAGA TATGGATTTT TCAAGAGAGC AAGAGCAGCA GCATTTAACT
    AGCTACCACA CATTATCCTC AAGGCAAGAA AGAGTTTCTG AAAGTAAGGA AAGTTTGCTC
    TCAGACATTG ATCATTCGCA GAGTTGCAGA AAACAAATGG AGCAAAATCG CCGCTTACAG
    CAGCAAATGC TGGAGTGTGA TAAATCAGAT AAAACTGAAA GCACACCAAG CACTGATGCG
    GAGGAATTTG AGCATCGAAG TATTGTGCAT GAGGTTGTAA AACCATTACA AGATGTAACG
    GGAAGTTCTC CGACCAGCAA GCAAAAAAAA TTGGGAGGAT TCGAATTTGA TTTTGGCAAA
    AGAGAGCAAA ATTATGAAAT GTTTCGACTG AGAAATGATG AACCTGAAAG AGGACTTGCT
    TCACATCCAG GGACACCCCT TGCTGAGGAG GAGAGAAATG CATCACAATT GTTGGACAAA
    GACCCAGATT TACCAGTGAC AATGGATAAA AATAGCTCGC ATTTAGATGT TTCAAAATAC
    AATACTAGCA ATGCAGTGCA TCAAGGGCTT TCACCACATG CAGAGATACT AAAAGTGAAA
    CCATCAGTTA CTAAAGAGGA GTTCAGTTGG GAGAGCAATA TTAGGCAGGG CACATTGAGA
    GAAATGAGCT TTCCCACAAG CATTGTTAAA CGGGAAAGTA TCAGAAAACG TCCTGAACGC
    GAATTAGAAC CTGGAGAGGT TCAGTCAGAC TCTGATGAAG ATGAAAGCAG ACACTATTCA
    TTAAAGCCAA TTTCTTTCAA AAGAGAGCAT GAAGAGCGGC TTTCAGATGT AAAGTATTCT
    GAGTCTCTTG AAAAGAATAA GTTCTATGAA TTTGCATTGG ATAAGACGAT AACACCAGAC
    ACAAAAGCTT TGCTTGAGCG TGCTAAGTCA CTGTCTTCAT CTAGAGAAGA AAATTGGTTT
    TTCCTTAATC ATGACTCAAA ATTCAAAAGT TTACAGAATA ACATCGACAA GGAAAAGTCT
    GAACCTACTC CAAGACCTAT TCCTTCATGG TACTTGAAGA AAAAGAAAAG TCGCTCTGAC
    TCTGATGGAA AACTTGATGA AAAAAAGCAA GATGCTAAGC CTGATGAACA AGAAAGACAA
    GATCTCCTAG CTTCACGATT TCTTCATAGC TCAATCTTTG AACAGGATTC ACGTCGGCTT
    CAACACCTTG AACGGAAAAA TACTGACCCT GACGTTAGAA TTGGCAGAGA TAGTGTTACA
    AGTAATTCTC AAGGTGAACA ACCTGGAACA GGAGGATCAG ACTTGACTCA AGAACCAAGA
    GTACTTTTTC ATAGCCGCTT TTTGGAACTT CAGCAAAGAG ACAAGAACCA ACAGCTTCCT
    ATTTCAGAAA AACTTAGTTT AACTGATCAA ATGGAGAAGA CATTGGATGA AGAGTTTAGG
    CCATCTCCCA ATTTATCAGA CATTTCCCAA GAGTCATTAG CAAGTCATAG CTTAACACCT
    ACAATATCCC CAGTGTCTCA GTCTAAAATT TCAGAGATGT CTGTGCACCA TGAGAAGCCT
    GTTTTGCATA CTACTTTACC AAACATACAT AATCCAGCAG TTGTGAATGT TAGGTCAGAA
    AATGCTCCAA TAGATTATCC ATCGTTAATT AGCCTAAAGG ATGAGAACAA CACTATTCCG
    TTGAGCACTC CATCACCTGA GCCCATGATA AAGGAAGATG TAATTGTTGA AGAACCTGAA
    GAAAAACTCA GAGATTCCAG AAAAACTGTA CACGCCGCTG TTGAACACGA GATTGAGATC
    AAACCTCCCA CTCCTGGTGC ATCTTTAGGT AATGTTGAGC CAGAATGTGA TGCTCTGCAG
    GCATCTTCTC CATTGTATCC AAATCCCAGT AAAGATCAAG AGACTACAGA GCTCTCAGAA
    ACAACAACAG AGCCTGCTAA TTCTTCTCTT GTTGTATCTC CTCTGGAGGT GCCAGACACA
    GAAATTGAGC AAGTTGAACA AGTACTACCA CCGCGTAAAC AACCAAAGAG TAAAAAGGTA
    AAGAATGTCT CATCAGCGCA AACACCAATC ACCCAAGTCG TTAATGAAAA ACCAGCTACA
    CGAAAGAGTG AACGCATTGA TAAAGAAAAG CTAAAACGAT CCCCCAGAGG AGACTCTACA
    AAACTTACTG CTGATTCCAG AAATTCTGCA AAATCTCCTG TTCAAGTTTT AGATTCAGAG
    CAGGGCCTTG AATTAAACAC AAACCAAGGC AGAACACGCC AACGACGGAA TGTGCGATCT
    GTCTATGCAA CACCCCCAGA GGAAGAATCT CCTCAACAAC AAGGAAAAGA GGTAACAGAA
    CCTCCCCGTT CCACTCTTAA GCGCTCTAGG GGTCGTCCAC CCAAGACTCG CAGACGAGTG
    GATGATATGT CTCCGGTAAA AGGAGATCAG ATCAAAAATT CTGAGGCTGA AGACACTGAA
    AATAAAGAGT CAACAAGTAG TGGAGAAATA ACTAAAAGTA CAGAGGCATG GCGCTCTCCA
    CGTTCTCAAA AGGGCCAGTC TTCACCGATA AGATCAGGAC AAAGCAAAAA GACATTGAAA
    TTGGACAAAG TGTCAGGTAC TTCGGCATGT GTTCAATTAG ACAGAGAAGC AGTTGATGTG
    CCACCTGCCC TAGATCGCAA AGCTGAATCC AAAGAAACTT TGATGCAAGT TGGACATTTG
    TCAAAAGATA CCAAACAGAA CAGCATATCT ACAAGAGAAA AAGAACTTGT TGCTGAGGAA
    ATTTCAACAG ATGACCGTGT AGAAATCACT CCTGTAGAAA AAATATTAGA CAAGGAGGTC
    AAGACATCAA GGTCAACACG GAACACAAAA ACAATGTCTG ATAATAAGGC TGTCAATGTG
    GTGAAGCTCA CTATGGATGC AGTTAAGGAA GCTGTTCATT CAGGTGATGA CATAACTGTT
    TGTTTTGAAG GGTCTTTAAA AGCTAATGTT CCACAATCAG TAATAGAACA GTCAGACATT
    CAAGATTATC ACAAAAAGGA AAATGTTAAC AGCCTTGATG AAAAGGATGA GACTCTTTCA
    GAAATGGAGC CTCCTACTGA TCCTGTGGCT GCCCTGCTTG CTCGCCAAAT GGAACTGGAA
    AGAGCAGTGG AAAATATTTC AAGATTTACA GATGACCAGC ATCCAGTACC TTACAAAGAA
    CCTCGTACAG AACCACCTAC CCTAACAGCA CCTGTTGTAG TACAACCTGC AGAGGATACA
    GAGGTAGGAA AGCCTGCCAA TCCAGCAAGT GAAACTGAAT TGGCTGAAGC AATTAATTCA
    ATTACTGCTG AGGATATATC AGGTGATACA GATGGATTCT CTGCTTCAAC AACATACACA
    ACCCTCCTTC CTCAACCAGA GACTCTTGAT TTGCCTGTAC CAAATGAGGT TGTGGAAACT
    GAGTCAGACT TGACTGTAAA GACTATTGCA CAGTCTGAAC AAGATAATGT TATGGTTCCA
    GTTTCAAAAT CTTCTAAATG TAAAACAGAT ACTTCACTCA AAGAATCACC ATTGACAGAG
    GTAGCAAAAA GAGGAGGAAG AGCACGTCCA AAAACTGCAA AAAAATCCAG AGGGAGGAAG
    GTTTCTTTGA ACAGAAAATT AGATATTGCT GAGGATGTAG TGCTGGAACC TGAATCCACT
    ACAGTCAAAC TTCCAGAATC GATTCCAGAA GAGATTCAAA CAGTTAACCC TAAGGCAGCA
    ACATCGGCAG CTGCAGCAGC TGTAGTCACA GTTGCTGCTG CATGCAAACA TGAAGCTGCA
    TCTACAGTGA TTTTGAATAC ACCAAAAGAG GCAGAACAGC CTGCAGTTGA TCAGCCTGAA
    CCTCAAGAAT CTGCCTTCCA TTCTGGAAAT AACAGTCCTT CTTATTTGAG GACACAACAG
    TCATCTCCTG AGCGAGGAGC ATCTGCCCTT ACTTCACCTA CAACACGCCT AAATTCACCT
    GCATCCTCTA ACATTCCTCC TGAATGGAAT ACCAGGACAG AGGAGAAAGT AATGCTACCT
    AAACCACAAG GTAATGTGGC TTTGTCTATC CCAGGAGCTG GGGGACCCCC AGCAAATCCT
    CCCATGCCTC CTGACACAAA AGCCTCTGAT ATTAATGCAA GTTCTAGTAC ACTGCGAAAA
    ATTTTAATGG AACCAAAGTA TGTTTCTGCT TCAAATAGCA ATTCAGTGAC AAGTATGCAG
    TTTACTACCA CATTAGCAGA TCCACGGATG TCTGATAATG AAAATTCAGT TGAGGCTGTG
    CTGCCTTTAA AAACCTCTCT ACCTGAAGAT AGACCTAGCC CTATAGCTCA GCTTGTACCC
    CGTACAACAC CACCGCAGCC CCCTCCTCTA CAACAATGTG AGACTCCACA GATCTTAAAG
    GAGAAACTAG CTATAACTTC CACTGCCACT TCAGTCATTA GTCGGATCCC TATGCCCTTT
    GATTTTGAGG ACACTCCTCG CATCTCTTTA AGTAATCGTA ACTCTGGAAT GTCTTTGCCA
    AAGCAGAAAT ATCGTACTGG CTTGAATGAA AATAATAGAT ATCATGGACA TAATACTTCT
    GAGGATGGTG GAAGTGTTGG GCGCCCAGTT GTTGAAGGCA CACATTGTAA CACGGGGTCT
    AGTACTGGTT TGAGGGTCAA CACATCTGAG GGTGTGGTAG TATTAAGTTA TTCTGGACAA
    AAGACAGAGG GACCACAGCG GATCATTGCA AAAATTAGCC AAATCCCTCC AGCCAGTGCA
    GTTGACATTG AATTTCAGCA GTCTATAACA AAATCTCAGA TTAAACAAGA ACCTTCATCT
    CACCCCTCTA CCCCAAAAGG ATCACAGACA CCTACAGGCT ATAGTCACAC AGGGGTAGTA
    TTAGCTGGCC AAAGTATCAA TGCCCAACCT GTTATATCCT CAATACGCCA GGAAAGCCCT
    GGCTCTGAAA AATCAGAAAC ATCTTACCAA CAAGGAAGTT CTCTGAAATC TTTCCACCAG
    TCCCCAAGCC ATCCTCAACT TCTGAGGTAT GGTCAGTCAA TAACACCGCA ACACCATATG
    AAGAAAAATG GAGAAACTGA ATCATTTTCA ATGAAAGCTG ATATAAAACC TGATATAAAG
    GCAACACAGA CTTTCAATGT TAAACCAGTT TTAAGTCCAC GCCACCCATC TTTCTCAGGC
    AATCACATTT TAAGTCCAAG TGCTCCACAT GAAAGAGCTG TCCCACAACC CAAGCAAGAT
    TCACATTCTC CAAGGGCATC TAACCATTCC ATGTCACCCT TTCCAAAGGT TTGCCCCCCT
    AACAGCCCCG TTGTTCTGGG ACCAGCTGGC CCTATGTCTC AGTATGTGTC AAATATACAC
    CATGCTGAGC AGTCAGTGAT CATGCCTCCT CATAGTGTCA CCCAGTCTGT GCCCATGAGT
    CATTTGTCCC AAGGGGATGT CCGAGCCAGT ACTCCAACAT TATCTGGAAT AGGTTATGGA
    TTGCGTTCAG AAAATCTCTT ATCACCTCGA TCGGCACCTC CTCAACGTTC CACGACTCCT
    CAACCTGCAG TGATCAGAGA TGTCATATTG AAATCGCATG CAGGCTCGTC TGTTGGTGGC
    CATGTTGTTG AGACGAGCAA TGATGATGCA CAGAATCTCC CACAAGGACT TCGTAGATCT
    TCTGCCCCAC AGTTGCAACA GGAAAGTATG GTAATACAGT CGGAATTCAA AAATCTGCAT
    CACAGGGCAT TACGTCTAGA TCAGTATGCC AGACTTATGC AGCAGCATTT GACTGACCAT
    CCAGGTGTTG TTGAAAGTCG CCAAACTCGA ACTGCAGAGG TGGTGCAGTC ATCTTCACAC
    ATATCATCTG CTTCTTCTAA AGCCTCTTCC ATAGTAAAGA ATGCTCCACA GATTGTAAGG
    GATGGACAAA AAACAGTGGA GCTGAAGATG TCACCCTCCC CTCATTCAGA CAACAGGATA
    ACAGGACACC CACCAGGCTC AGTGATGGTA TCTCCTCAAG GAGTTCAGTT AAATTACCCT
    GGAAATGGAA ATACCATGAA TGAATACTAC AAAGAAATGC GTGGCTTCCA TCCTCAGTAT
    CCAGGTCATT CAGTAATTGG AATTAACTTG GCTAACCGTG GGATTCCTGT GTCTCAGGTC
    TCACAAATTG ATCACAGTCA AAGACACAAG GTTCCCTCTC TCTCTTCCAG TGAGTCTGTG
    GGAAGTTTTA GTGAGTCAAA ACTTGAGGGT TCTCACATTC GACACTCAGG CACCATGGAT
    TTGTCTCATA TATCACGAGT TCAGAGTGAG GCTGGGTCCC CCTCTTATAT TTCTCCAGTG
    ACTATAACAC CCAAACTGGA GTTGGCTATT ACTTTGCAGA AGGGGCCTCA AGGACCTGTT
    TCCAACAAGA TGCCACTTCC TTCTTTAGCT TCTTCACAGA TGCGTTCAGA CTTCAAACTT
    GACCACACAG GACTTCGGTC TGTTGATATG GTGCAGTTGT TAACGAAGTA CCCAATCATT
    TGGCAAGGTC ACCTGGCACT TAAGAATGAC ACTGCAGCAG TGCAGCTGCA CTTTGTGTCT
    GGTAATAATG TTCTGGCTCA TCGTTCACTG CCACCCCCAG AAGGAGGTGC CTTTCTTCGC
    ATTGCTCAGA GAATGCGCTT AGAGGCTTCA CAACTGGAAG GAGTTGCGAG ACGCATGACT
    GCTGAAAATG AATACTGCCT TCTGTTGGCA TTGCCTTGTG GATTGGACCA AGAAGATGTC
    CACAACCAGA CCCATGCTCT TAAAACTGGT TTTATTACTT ACCTGCAAGC AAAACAGGCT
    GCTGGAATTA TCAATGTCCC AAACCCAGGC TCAAATCAGC CTGCCTACGT TGTCCAGATT
    TTCCCTCCTT GTGAGTTTTC TGAGAGCCAC CTGTCTCATC TTGCTCCGGA CCTTCTCAAC
    AGCATCTCCA GTATCTCTCC TCATCTTATG ATTGTTATAG CTTCTGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41575
    Clone ID Related Accession (Same CDS sequence) XM_009296917.3 , XM_009296917.2
    Accession Version XM_009296917.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product msx2-interacting protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    ATGGTCCGGG AAACCAGACA TTTATGGGTG GGAAATTTAC CAGAAAATGT ACGAGAGGAA 
    AAAATTATCG AGCACTTCAA ACGGTATGGA CGAGTGGAGA GTGTCAAGGT TCTCCCTAAA
    CGTGGATCGG AAGGTGGAGT GGCAGCTTTT GTGGATTTTG TGGACATTAA GAGTGCTCAG
    AAAGCTCATA ATTCTATCAA CAAGATGGGA GACAGGGACC TTCGCACCGA TTACAATGAA
    CCAGGAACTA TACCCAGTGC AGCTCGAGGC CTGGATGATA GCCTGTCCAT AGCCACTCGT
    GGGCGGGATG TTTCAGGGTT CACAAGGGGG GCAGGGGGCC CTGTGTATGG GCCCTCTGTG
    TCACTTCACA GCAGAGAGGG ACGCTTTGAA CGCAGACTAG ACGGGACTTC GGACAGCCGG
    GAACGCTCTT ACGATCACAG TGCCTATGGA CACCATGAGC GTAGCAGCAG CAACAGCACT
    AGTTTCGATC GGCAGCGTCA CTATGAGAGC GATTATTACA GAGACTCTAG GGACCGGGCC
    CTCAGTGGAA CCAGTGGGAG TGCCAGTTCA GCCAGTGGAA GCATCGGAGG AAGCTCATCT
    GCGGGCAGTA TAGGTGTTGC AGGGAATGTT GCAGGAAGCA GTGGTGCTGG TGGAAGTACT
    GCCGCTGGAG TAGGCAGTGG TGGCTCCACA CCAGGAGGAA TTGTCTATTA TGGTTCACGA
    AGCCGGAGCC CCAGTCGTTT TGAGACTACA GAGACTCGTT ATGAGCCCCG TGCCCGTGAA
    GCTTTCACAC TTGCCAGTGT GGTTCATCGG GATTTATTCA GAGAAGAAAG AGGCAGACGT
    GGGGAAAGGA CATACCACCA CAGTCGCAGT AGATCACCAC ACTCTTCACA GTCACACAAT
    CCATCACCAC AGAGGTTGCA AAGTCAGGCA GCGCGACCGG CACGCTCCCG CAGTGGCTCA
    GGATCCCGCA GTCGCTCCTC CAGCTCAGAC TCCGTCAGCA GTACCAGCAG CAGTGGCAGT
    GGCAGTGATT CCAGCAGCAC CTCCAGTGAC CGATCTCCAG CTCGATCTGT TCAGTCCACT
    GCAGTTCCTG CCCCTTCTAC ACAGCCACTA CCTTCACTGG ACAAAGATGA ACCTCGTAAA
    AGTTTTGGTA TCAAAGTGCA GAACCTGCCC GTGCGCTCAA CAGATACAAG CCTCAAGGAT
    GGTCTTTTCC ATGAATTTAA GAAACATGGT AAAGTCACAT CAGTTCAGAT CCACGGTGCC
    TCTGAGGAAC GATATGGCTT AGTGTTCTTT CGCCAGCAAG AGGACCAAGA AAAAGCTCTA
    AGTGCATCTA AAGGGAAGCT GTTTTTTGGC ATGCAAATTG ATGTCACTGC TTGGCATGGC
    CCAGAAACAG AAAGTGAGAA CGAGTTCCGG CCTTTGGATG AACGCATAGA TGAGTTTCAT
    CCCAAAGCTA CAAGGACATT GTTCATAGGA AATTTGGAAA AAACCACAAC CTATAATGAC
    CTGCTGAACA TATTTCAGCG TTTTGGAGAG ATTGTTGATA TTGATATTAA AAAGGTAAAT
    GGTTCCCCAC AATATGCCTT TCTCCAGTAT TGTGACATAG CAAGTGTCTG CAAAGCAATT
    AAGAAAATGG ATGGTGAATA TCTTGGCAAC AACAGGTTAA AGCTTGGTTT TGGCAAGAGT
    ATGCCTACTA CATGTGTATG GTTGGATGGC TTGTCCTCCA GTATTACTGA ACAGTATCTC
    ACGAGGCACT TTTGTCGTTA TGGACATGTA GTAAAGGTTG TCTTTGACCG GCTGAAAGGA
    ATGGCCCTCA TCTTATATAA TAATATAGAG TATGCGCAGG CTGCTGTCAA GGAAACAAAG
    GGTTGGAAAA TTGGAGGAAA TAAAATAAAG GTTGACTTTG CTAACCAGGA AAGTCAGATG
    GCTTTCTACC GTTCAATGCA GGGATCTGGA CAAGACATAA GAGATTTCTA TGAAATCATA
    TCTGAACGAA GAGATGAGCG CCGGACGCCA TATCACGAGT TCACAGCTGA ACGTGCATAC
    TATGAAAATG TGCGTACCCC AACTTCTTAC ACAGAGGATC CACGTCGAAA ATATCCTGCC
    AGAAGTCGAG AGTTTTTTTC AGAATGGGAT CCCTACCAGG GAGATTACTA TGACCCACGA
    TATTATGATG ACTCCCGTGA ATACAGGGAT TTTAGAGACC CATATGAGCA GGACATTAGA
    AAGTACAGCT ACTTGCAAAG AGAACGTGAA AGGGAAAGAG AGAGGTTTGA AACAGATCGT
    GAGCGAGACC ATGGTCGACG AACAATTGAA CACCAACAAA GCCCAACCCA TTCACGTCGT
    CCTGCTAGTC CAACTGCTTC TCCATCCCTT TCTGAACGTC CTCCCAGTGA CTCTGAACAT
    CACGTTTACA GTCGCTCATC TGAGCGAAGT GGTAGCTGCA GCTCACTTTC TCCACCACGC
    TTTGAGAAGC CAGACAAAAT TCGTTTGGAG AGGCACAATA GGAATGATAA ATTGGAGAAA
    GAAAAATCAC TTTTTGAGAC AGAGCGTGGA AATGGTGGGG AAAAAGAAAG GCGAGCAGGA
    CGTAAGGAAA AAGGAGAAAA GGACAGGACT GAGAGACAGA AGTTGAAGAA GTTAAAACTT
    GCATCTCCCA CTGTTCCATC ACCAGAGGCA GAGCTGGAAC TTGACAGGGA TGCAAGCCCA
    GAGGCTAACA TAACTCTGCG AGGTAAAGTG AGCAAATCAT TGATCAAAGA CAAAGACTAC
    TCTGGTAAAG GAAAACTTGA TCTGCTACCT TGTGTTGTCC AGCTAACAAG AGTAAAAGAG
    AAGGAAGGAA AATTAATAGA CAGTATCCTC TGTGAAAAAC AAAAAACAAA GGTTGGAAGT
    GATCCTGTTC TGTCTCCAAC AACACCTTCC TCAGGTGATC ACAAGAGTAT GCCTTTTCGC
    AAAGACACTC AGGCTAGAGA TTTCTTTAAG CATGGAAAAC ATCTCAAGGA GAAAGGCCTG
    GCAAGCCAAG TTGAGGTAGT GGATAAAGAG GAAAAAGTGA AAAACAAAAA ATATTTCAAA
    TCTGATCTTG CATTTGAATC CAGCTCTTCT GTGGATGCTG ACCGCAAGGC TGCACGAAAG
    AGACGCTTTG AAGAAACATC GGCTAAGGCT GATAATTTAA GGAGAGTAAG TCAGGAGGAG
    GATGAAGGGA AATTAAGGAG AATTATTGAT GAACCTTTGC TGAAAGACAC TGATTATGAT
    AAAAAGTTGC TTCGGAAAGA AGCACACAAG AGAGAGCGAA AAATTAAACC AGAGAGGATG
    GTTACTGTTA GTACTACAAT AGAAGAGTTA GACAATGTAA TGCCAGTAGG CCCCAGTTTA
    GACCTTCAGG CTCGACTTGG GGAACCAGCT GAAGATGCAA TAGATCCATT AGATAGCCTT
    GATCAAAAGA TAGGTGCTTT TGGAGCTAAC AGACAACCAA GTTTCAGTCT TGCAGTCTCC
    GACGATGGAA GTGTAGATAT GGATTTTTCA AGAGAGCAAG AGCAGCAGCA TTTAACTAGC
    TACCACACAT TATCCTCAAG GCAAGAAAGA GTTTCTGAAA GTAAGGAAAG TTTGCTCTCA
    GACATTGATC ATTCGCAGAG TTGCAGAAAA CAAATGGAGC AAAATCGCCG CTTACAGCAG
    CAAATGCTGG AGTGTGATAA ATCAGATAAA ACTGAAAGCA CACCAAGCAC TGATGCGGAG
    GAATTTGAGC ATCGAAGTAT TGTGCATGAG GTTGTAAAAC CATTACAAGA TGTAACGGGA
    AGTTCTCCGA CCAGCAAGCA AAAAAAATTG GGAGGATTCG AATTTGATTT TGGCAAAAGA
    GAGCAAAATT ATGAAATGTT TCGACTGAGA AATGATGAAC CTGAAAGAGG ACTTGCTTCA
    CATCCAGGGA CACCCCTTGC TGAGGAGGAG AGAAATGCAT CACAATTGTT GGACAAAGAC
    CCAGATTTAC CAGTGACAAT GGATAAAAAT AGCTCGCATT TAGATGTTTC AAAATACAAT
    ACTAGCAATG CAGTGCATCA AGGGCTTTCA CCACATGCAG AGATACTAAA AGTGAAACCA
    TCAGTTACTA AAGAGGAGTT CAGTTGGGAG AGCAATATTA GGCAGGGCAC ATTGAGAGAA
    ATGAGCTTTC CCACAAGCAT TGTTAAACGG GAAAGTATCA GAAAACGTCC TGAACGCGAA
    TTAGAACCTG GAGAGGTTCA GTCAGACTCT GATGAAGATG AAAGCAGACA CTATTCATTA
    AAGCCAATTT CTTTCAAAAG AGAGCATGAA GAGCGGCTTT CAGATGTAAA GTATTCTGAG
    TCTCTTGAAA AGAATAAGTT CTATGAATTT GCATTGGATA AGACGATAAC ACCAGACACA
    AAAGCTTTGC TTGAGCGTGC TAAGTCACTG TCTTCATCTA GAGAAGAAAA TTGGTTTTTC
    CTTAATCATG ACTCAAAATT CAAAAGTTTA CAGAATAACA TCGACAAGGA AAAGTCTGAA
    CCTACTCCAA GACCTATTCC TTCATGGTAC TTGAAGAAAA AGAAAAGTCG CTCTGACTCT
    GATGGAAAAC TTGATGAAAA AAAGCAAGAT GCTAAGCCTG ATGAACAAGA AAGACAAGAT
    CTCCTAGCTT CACGATTTCT TCATAGCTCA ATCTTTGAAC AGGATTCACG TCGGCTTCAA
    CACCTTGAAC GGAAAAATAC TGACCCTGAC GTTAGAATTG GCAGAGATAG TGTTACAAGT
    AATTCTCAAG GTGAACAACC TGGAACAGGA GGATCAGACT TGACTCAAGA ACCAAGAGTA
    CTTTTTCATA GCCGCTTTTT GGAACTTCAG CAAAGAGACA AGAACCAACA GCTTCCTATT
    TCAGAAAAAC TTAGTTTAAC TGATCAAATG GAGAAGACAT TGGATGAAGA GTTTAGGCCA
    TCTCCCAATT TATCAGACAT TTCCCAAGAG TCATTAGCAA GTCATAGCTT AACACCTACA
    ATATCCCCAG TGTCTCAGTC TAAAATTTCA GAGATGTCTG TGCACCATGA GAAGCCTGTT
    TTGCATACTA CTTTACCAAA CATACATAAT CCAGCAGTTG TGAATGTTAG GTCAGAAAAT
    GCTCCAATAG ATTATCCATC GTTAATTAGC CTAAAGGATG AGAACAACAC TATTCCGTTG
    AGCACTCCAT CACCTGAGCC CATGATAAAG GAAGATGTAA TTGTTGAAGA ACCTGAAGAA
    AAACTCAGAG ATTCCAGAAA AACTGTACAC GCCGCTGTTG AACACGAGAT TGAGATCAAA
    CCTCCCACTC CTGGTGCATC TTTAGGTAAT GTTGAGCCAG AATGTGATGC TCTGCAGGCA
    TCTTCTCCAT TGTATCCAAA TCCCAGTAAA GATCAAGAGA CTACAGAGCT CTCAGAAACA
    ACAACAGAGC CTGCTAATTC TTCTCTTGTT GTATCTCCTC TGGAGGTGCC AGACACAGAA
    ATTGAGCAAG TTGAACAAGT ACTACCACCG CGTAAACAAC CAAAGAGTAA AAAGGTAAAG
    AATGTCTCAT CAGCGCAAAC ACCAATCACC CAAGTCGTTA ATGAAAAACC AGCTACACGA
    AAGAGTGAAC GCATTGATAA AGAAAAGCTA AAACGATCCC CCAGAGGAGA CTCTACAAAA
    CTTACTGCTG ATTCCAGAAA TTCTGCAAAA TCTCCTGTTC AAGTTTTAGA TTCAGAGCAG
    GGCCTTGAAT TAAACACAAA CCAAGGCAGA ACACGCCAAC GACGGAATGT GCGATCTGTC
    TATGCAACAC CCCCAGAGGA AGAATCTCCT CAACAACAAG GAAAAGAGGT AACAGAACCT
    CCCCGTTCCA CTCTTAAGCG CTCTAGGGGT CGTCCACCCA AGACTCGCAG ACGAGTGGAT
    GATATGTCTC CGGTAAAAGG AGATCAGATC AAAAATTCTG AGGCTGAAGA CACTGAAAAT
    AAAGAGTCAA CAAGTAGTGG AGAAATAACT AAAAGTACAG AGGCATGGCG CTCTCCACGT
    TCTCAAAAGG GCCAGTCTTC ACCGATAAGA TCAGGACAAA GCAAAAAGAC ATTGAAATTG
    GACAAAGTGT CAGGTACTTC GGCATGTGTT CAATTAGACA GAGAAGCAGT TGATGTGCCA
    CCTGCCCTAG ATCGCAAAGC TGAATCCAAA GAAACTTTGA TGCAAGTTGG ACATTTGTCA
    AAAGATACCA AACAGAACAG CATATCTACA AGAGAAAAAG AACTTGTTGC TGAGGAAATT
    TCAACAGATG ACCGTGTAGA AATCACTCCT GTAGAAAAAA TATTAGACAA GGAGGTCAAG
    ACATCAAGGT CAACACGGAA CACAAAAACA ATGTCTGATA ATAAGGCTGT CAATGTGGTG
    AAGCTCACTA TGGATGCAGT TAAGGAAGCT GTTCATTCAG GTGATGACAT AACTGTTTGT
    TTTGAAGGGT CTTTAAAAGC TAATGTTCCA CAATCAGTAA TAGAACAGTC AGACATTCAA
    GATTATCACA AAAAGGAAAA TGTTAACAGC CTTGATGAAA AGGATGAGAC TCTTTCAGAA
    ATGGAGCCTC CTACTGATCC TGTGGCTGCC CTGCTTGCTC GCCAAATGGA ACTGGAAAGA
    GCAGTGGAAA ATATTTCAAG ATTTACAGAT GACCAGCATC CAGTACCTTA CAAAGAACCT
    CGTACAGAAC CACCTACCCT AACAGCACCT GTTGTAGTAC AACCTGCAGA GGATACAGAG
    GTAGGAAAGC CTGCCAATCC AGCAAGTGAA ACTGAATTGG CTGAAGCAAT TAATTCAATT
    ACTGCTGAGG ATATATCAGG TGATACAGAT GGATTCTCTG CTTCAACAAC ATACACAACC
    CTCCTTCCTC AACCAGAGAC TCTTGATTTG CCTGTACCAA ATGAGGTTGT GGAAACTGAG
    TCAGACTTGA CTGTAAAGAC TATTGCACAG TCTGAACAAG ATAATGTTAT GGTTCCAGTT
    TCAAAATCTT CTAAATGTAA AACAGATACT TCACTCAAAG AATCACCATT GACAGAGGTA
    GCAAAAAGAG GAGGAAGAGC ACGTCCAAAA ACTGCAAAAA AATCCAGAGG GAGGAAGGTT
    TCTTTGAACA GAAAATTAGA TATTGCTGAG GATGTAGTGC TGGAACCTGA ATCCACTACA
    GTCAAACTTC CAGAATCGAT TCCAGAAGAG ATTCAAACAG TTAACCCTAA GGCAGCAACA
    TCGGCAGCTG CAGCAGCTGT AGTCACAGTT GCTGCTGCAT GCAAACATGA AGCTGCATCT
    ACAGTGATTT TGAATACACC AAAAGAGGCA GAACAGCCTG CAGTTGATCA GCCTGAACCT
    CAAGAATCTG CCTTCCATTC TGGAAATAAC AGTCCTTCTT ATTTGAGGAC ACAACAGTCA
    TCTCCTGAGC GAGGAGCATC TGCCCTTACT TCACCTACAA CACGCCTAAA TTCACCTGCA
    TCCTCTAACA TTCCTCCTGA ATGGAATACC AGGACAGAGG AGAAAGTAAT GCTACCTAAA
    CCACAAGGTA ATGTGGCTTT GTCTATCCCA GGAGCTGGGG GACCCCCAGC AAATCCTCCC
    ATGCCTCCTG ACACAAAAGC CTCTGATATT AATGCAAGTT CTAGTACACT GCGAAAAATT
    TTAATGGAAC CAAAGTATGT TTCTGCTTCA AATAGCAATT CAGTGACAAG TATGCAGTTT
    ACTACCACAT TAGCAGATCC ACGGATGTCT GATAATGAAA ATTCAGTTGA GGCTGTGCTG
    CCTTTAAAAA CCTCTCTACC TGAAGATAGA CCTAGCCCTA TAGCTCAGCT TGTACCCCGT
    ACAACACCAC CGCAGCCCCC TCCTCTACAA CAATGTGAGA CTCCACAGAT CTTAAAGGAG
    AAACTAGCTA TAACTTCCAC TGCCACTTCA GTCATTAGTC GGATCCCTAT GCCCTTTGAT
    TTTGAGGACA CTCCTCGCAT CTCTTTAAGT AATCGTAACT CTGGAATGTC TTTGCCAAAG
    CAGAAATATC GTACTGGCTT GAATGAAAAT AATAGATATC ATGGACATAA TACTTCTGAG
    GATGGTGGAA GTGTTGGGCG CCCAGTTGTT GAAGGCACAC ATTGTAACAC GGGGTCTAGT
    ACTGGTTTGA GGGTCAACAC ATCTGAGGGT GTGGTAGTAT TAAGTTATTC TGGACAAAAG
    ACAGAGGGAC CACAGCGGAT CATTGCAAAA ATTAGCCAAA TCCCTCCAGC CAGTGCAGTT
    GACATTGAAT TTCAGCAGTC TATAACAAAA TCTCAGATTA AACAAGAACC TTCATCTCAC
    CCCTCTACCC CAAAAGGATC ACAGACACCT ACAGGCTATA GTCACACAGG GGTAGTATTA
    GCTGGCCAAA GTATCAATGC CCAACCTGTT ATATCCTCAA TACGCCAGGA AAGCCCTGGC
    TCTGAAAAAT CAGAAACATC TTACCAACAA GGAAGTTCTC TGAAATCTTT CCACCAGTCC
    CCAAGCCATC CTCAACTTCT GAGGTATGGT CAGTCAATAA CACCGCAACA CCATATGAAG
    AAAAATGGAG AAACTGAATC ATTTTCAATG AAAGCTGATA TAAAACCTGA TATAAAGGCA
    ACACAGACTT TCAATGTTAA ACCAGTTTTA AGTCCACGCC ACCCATCTTT CTCAGGCAAT
    CACATTTTAA GTCCAAGTGC TCCACATGAA AGAGCTGTCC CACAACCCAA GCAAGATTCA
    CATTCTCCAA GGGCATCTAA CCATTCCATG TCACCCTTTC CAAAGGTTTG CCCCCCTAAC
    AGCCCCGTTG TTCTGGGACC AGCTGGCCCT ATGTCTCAGT ATGTGTCAAA TATACACCAT
    GCTGAGCAGT CAGTGATCAT GCCTCCTCAT AGTGTCACCC AGTCTGTGCC CATGAGTCAT
    TTGTCCCAAG GGGATGTCCG AGCCAGTACT CCAACATTAT CTGGAATAGG TTATGGATTG
    CGTTCAGAAA ATCTCTTATC ACCTCGATCG GCACCTCCTC AACGTTCCAC GACTCCTCAA
    CCTGCAGTGA TCAGAGATGT CATATTGAAA TCGCATGCAG GCTCGTCTGT TGGTGGCCAT
    GTTGTTGAGA CGAGCAATGA TGATGCACAG AATCTCCCAC AAGGACTTCG TAGATCTTCT
    GCCCCACAGT TGCAACAGGA AAGTATGGTA ATACAGTCGG AATTCAAAAA TCTGCATCAC
    AGGGCATTAC GTCTAGATCA GTATGCCAGA CTTATGCAGC AGCATTTGAC TGACCATCCA
    GGTGTTGTTG AAAGTCGCCA AACTCGAACT GCAGAGGTGG TGCAGTCATC TTCACACATA
    TCATCTGCTT CTTCTAAAGC CTCTTCCATA GTAAAGAATG CTCCACAGAT TGTAAGGGAT
    GGACAAAAAA CAGTGGAGCT GAAGATGTCA CCCTCCCCTC ATTCAGACAA CAGGATAACA
    GGACACCCAC CAGGCTCAGT GATGGTATCT CCTCAAGGAG TTCAGTTAAA TTACCCTGGA
    AATGGAAATA CCATGAATGA ATACTACAAA GAAATGCGTG GCTTCCATCC TCAGTATCCA
    GGTCATTCAG TAATTGGAAT TAACTTGGCT AACCGTGGGA TTCCTGTGTC TCAGGTCTCA
    CAAATTGATC ACAGTCAAAG ACACAAGGTT CCCTCTCTCT CTTCCAGTGA GTCTGTGGGA
    AGTTTTAGTG AGTCAAAACT TGAGGGTTCT CACATTCGAC ACTCAGGCAC CATGGATTTG
    TCTCATATAT CACGAGTTCA GAGTGAGGCT GGGTCCCCCT CTTATATTTC TCCAGTGACT
    ATAACACCCA AACTGGAGTT GGCTATTACT TTGCAGAAGG GGCCTCAAGG ACCTGTTTCC
    AACAAGATGC CACTTCCTTC TTTAGCTTCT TCACAGATGC GTTCAGACTT CAAACTTGAC
    CACACAGGAC TTCGGTCTGT TGATATGGTG CAGTTGTTAA CGAAGTACCC AATCATTTGG
    CAAGGTCACC TGGCACTTAA GAATGACACT GCAGCAGTGC AGCTGCACTT TGTGTCTGGT
    AATAATGTTC TGGCTCATCG TTCACTGCCA CCCCCAGAAG GAGGTGCCTT TCTTCGCATT
    GCTCAGAGAA TGCGCTTAGA GGCTTCACAA CTGGAAGGAG TTGCGAGACG CATGACTGCT
    GAAAATGAAT ACTGCCTTCT GTTGGCATTG CCTTGTGGAT TGGACCAAGA AGATGTCCAC
    AACCAGACCC ATGCTCTTAA AACTGGTTTT ATTACTTACC TGCAAGCAAA ACAGGCTGCT
    GGAATTATCA ATGTCCCAAA CCCAGGCTCA AATCAGCCTG CCTACGTTGT CCAGATTTTC
    CCTCCTTGTG AGTTTTCTGA GAGCCACCTG TCTCATCTTG CTCCGGACCT TCTCAACAGC
    ATCTCCAGTA TCTCTCCTCA TCTTATGATT GTTATAGCTT CTGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295192.1
    CDS1371..11798
    Translation

    Target ORF information:

    RefSeq Version XM_009296917.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spen family transcriptional repressor (spen), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296917.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    ATGGTCCGGG AAACCAGACA TTTATGGGTG GGAAATTTAC CAGAAAATGT ACGAGAGGAA 
    AAAATTATCG AGCACTTCAA ACGGTATGGA CGAGTGGAGA GTGTCAAGGT TCTCCCTAAA
    CGTGGATCGG AAGGTGGAGT GGCAGCTTTT GTGGATTTTG TGGACATTAA GAGTGCTCAG
    AAAGCTCATA ATTCTATCAA CAAGATGGGA GACAGGGACC TTCGCACCGA TTACAATGAA
    CCAGGAACTA TACCCAGTGC AGCTCGAGGC CTGGATGATA GCCTGTCCAT AGCCACTCGT
    GGGCGGGATG TTTCAGGGTT CACAAGGGGG GCAGGGGGCC CTGTGTATGG GCCCTCTGTG
    TCACTTCACA GCAGAGAGGG ACGCTTTGAA CGCAGACTAG ACGGGACTTC GGACAGCCGG
    GAACGCTCTT ACGATCACAG TGCCTATGGA CACCATGAGC GTAGCAGCAG CAACAGCACT
    AGTTTCGATC GGCAGCGTCA CTATGAGAGC GATTATTACA GAGACTCTAG GGACCGGGCC
    CTCAGTGGAA CCAGTGGGAG TGCCAGTTCA GCCAGTGGAA GCATCGGAGG AAGCTCATCT
    GCGGGCAGTA TAGGTGTTGC AGGGAATGTT GCAGGAAGCA GTGGTGCTGG TGGAAGTACT
    GCCGCTGGAG TAGGCAGTGG TGGCTCCACA CCAGGAGGAA TTGTCTATTA TGGTTCACGA
    AGCCGGAGCC CCAGTCGTTT TGAGACTACA GAGACTCGTT ATGAGCCCCG TGCCCGTGAA
    GCTTTCACAC TTGCCAGTGT GGTTCATCGG GATTTATTCA GAGAAGAAAG AGGCAGACGT
    GGGGAAAGGA CATACCACCA CAGTCGCAGT AGATCACCAC ACTCTTCACA GTCACACAAT
    CCATCACCAC AGAGGTTGCA AAGTCAGGCA GCGCGACCGG CACGCTCCCG CAGTGGCTCA
    GGATCCCGCA GTCGCTCCTC CAGCTCAGAC TCCGTCAGCA GTACCAGCAG CAGTGGCAGT
    GGCAGTGATT CCAGCAGCAC CTCCAGTGAC CGATCTCCAG CTCGATCTGT TCAGTCCACT
    GCAGTTCCTG CCCCTTCTAC ACAGCCACTA CCTTCACTGG ACAAAGATGA ACCTCGTAAA
    AGTTTTGGTA TCAAAGTGCA GAACCTGCCC GTGCGCTCAA CAGATACAAG CCTCAAGGAT
    GGTCTTTTCC ATGAATTTAA GAAACATGGT AAAGTCACAT CAGTTCAGAT CCACGGTGCC
    TCTGAGGAAC GATATGGCTT AGTGTTCTTT CGCCAGCAAG AGGACCAAGA AAAAGCTCTA
    AGTGCATCTA AAGGGAAGCT GTTTTTTGGC ATGCAAATTG ATGTCACTGC TTGGCATGGC
    CCAGAAACAG AAAGTGAGAA CGAGTTCCGG CCTTTGGATG AACGCATAGA TGAGTTTCAT
    CCCAAAGCTA CAAGGACATT GTTCATAGGA AATTTGGAAA AAACCACAAC CTATAATGAC
    CTGCTGAACA TATTTCAGCG TTTTGGAGAG ATTGTTGATA TTGATATTAA AAAGGTAAAT
    GGTTCCCCAC AATATGCCTT TCTCCAGTAT TGTGACATAG CAAGTGTCTG CAAAGCAATT
    AAGAAAATGG ATGGTGAATA TCTTGGCAAC AACAGGTTAA AGCTTGGTTT TGGCAAGAGT
    ATGCCTACTA CATGTGTATG GTTGGATGGC TTGTCCTCCA GTATTACTGA ACAGTATCTC
    ACGAGGCACT TTTGTCGTTA TGGACATGTA GTAAAGGTTG TCTTTGACCG GCTGAAAGGA
    ATGGCCCTCA TCTTATATAA TAATATAGAG TATGCGCAGG CTGCTGTCAA GGAAACAAAG
    GGTTGGAAAA TTGGAGGAAA TAAAATAAAG GTTGACTTTG CTAACCAGGA AAGTCAGATG
    GCTTTCTACC GTTCAATGCA GGGATCTGGA CAAGACATAA GAGATTTCTA TGAAATCATA
    TCTGAACGAA GAGATGAGCG CCGGACGCCA TATCACGAGT TCACAGCTGA ACGTGCATAC
    TATGAAAATG TGCGTACCCC AACTTCTTAC ACAGAGGATC CACGTCGAAA ATATCCTGCC
    AGAAGTCGAG AGTTTTTTTC AGAATGGGAT CCCTACCAGG GAGATTACTA TGACCCACGA
    TATTATGATG ACTCCCGTGA ATACAGGGAT TTTAGAGACC CATATGAGCA GGACATTAGA
    AAGTACAGCT ACTTGCAAAG AGAACGTGAA AGGGAAAGAG AGAGGTTTGA AACAGATCGT
    GAGCGAGACC ATGGTCGACG AACAATTGAA CACCAACAAA GCCCAACCCA TTCACGTCGT
    CCTGCTAGTC CAACTGCTTC TCCATCCCTT TCTGAACGTC CTCCCAGTGA CTCTGAACAT
    CACGTTTACA GTCGCTCATC TGAGCGAAGT GGTAGCTGCA GCTCACTTTC TCCACCACGC
    TTTGAGAAGC CAGACAAAAT TCGTTTGGAG AGGCACAATA GGAATGATAA ATTGGAGAAA
    GAAAAATCAC TTTTTGAGAC AGAGCGTGGA AATGGTGGGG AAAAAGAAAG GCGAGCAGGA
    CGTAAGGAAA AAGGAGAAAA GGACAGGACT GAGAGACAGA AGTTGAAGAA GTTAAAACTT
    GCATCTCCCA CTGTTCCATC ACCAGAGGCA GAGCTGGAAC TTGACAGGGA TGCAAGCCCA
    GAGGCTAACA TAACTCTGCG AGGTAAAGTG AGCAAATCAT TGATCAAAGA CAAAGACTAC
    TCTGGTAAAG GAAAACTTGA TCTGCTACCT TGTGTTGTCC AGCTAACAAG AGTAAAAGAG
    AAGGAAGGAA AATTAATAGA CAGTATCCTC TGTGAAAAAC AAAAAACAAA GGTTGGAAGT
    GATCCTGTTC TGTCTCCAAC AACACCTTCC TCAGGTGATC ACAAGAGTAT GCCTTTTCGC
    AAAGACACTC AGGCTAGAGA TTTCTTTAAG CATGGAAAAC ATCTCAAGGA GAAAGGCCTG
    GCAAGCCAAG TTGAGGTAGT GGATAAAGAG GAAAAAGTGA AAAACAAAAA ATATTTCAAA
    TCTGATCTTG CATTTGAATC CAGCTCTTCT GTGGATGCTG ACCGCAAGGC TGCACGAAAG
    AGACGCTTTG AAGAAACATC GGCTAAGGCT GATAATTTAA GGAGAGTAAG TCAGGAGGAG
    GATGAAGGGA AATTAAGGAG AATTATTGAT GAACCTTTGC TGAAAGACAC TGATTATGAT
    AAAAAGTTGC TTCGGAAAGA AGCACACAAG AGAGAGCGAA AAATTAAACC AGAGAGGATG
    GTTACTGTTA GTACTACAAT AGAAGAGTTA GACAATGTAA TGCCAGTAGG CCCCAGTTTA
    GACCTTCAGG CTCGACTTGG GGAACCAGCT GAAGATGCAA TAGATCCATT AGATAGCCTT
    GATCAAAAGA TAGGTGCTTT TGGAGCTAAC AGACAACCAA GTTTCAGTCT TGCAGTCTCC
    GACGATGGAA GTGTAGATAT GGATTTTTCA AGAGAGCAAG AGCAGCAGCA TTTAACTAGC
    TACCACACAT TATCCTCAAG GCAAGAAAGA GTTTCTGAAA GTAAGGAAAG TTTGCTCTCA
    GACATTGATC ATTCGCAGAG TTGCAGAAAA CAAATGGAGC AAAATCGCCG CTTACAGCAG
    CAAATGCTGG AGTGTGATAA ATCAGATAAA ACTGAAAGCA CACCAAGCAC TGATGCGGAG
    GAATTTGAGC ATCGAAGTAT TGTGCATGAG GTTGTAAAAC CATTACAAGA TGTAACGGGA
    AGTTCTCCGA CCAGCAAGCA AAAAAAATTG GGAGGATTCG AATTTGATTT TGGCAAAAGA
    GAGCAAAATT ATGAAATGTT TCGACTGAGA AATGATGAAC CTGAAAGAGG ACTTGCTTCA
    CATCCAGGGA CACCCCTTGC TGAGGAGGAG AGAAATGCAT CACAATTGTT GGACAAAGAC
    CCAGATTTAC CAGTGACAAT GGATAAAAAT AGCTCGCATT TAGATGTTTC AAAATACAAT
    ACTAGCAATG CAGTGCATCA AGGGCTTTCA CCACATGCAG AGATACTAAA AGTGAAACCA
    TCAGTTACTA AAGAGGAGTT CAGTTGGGAG AGCAATATTA GGCAGGGCAC ATTGAGAGAA
    ATGAGCTTTC CCACAAGCAT TGTTAAACGG GAAAGTATCA GAAAACGTCC TGAACGCGAA
    TTAGAACCTG GAGAGGTTCA GTCAGACTCT GATGAAGATG AAAGCAGACA CTATTCATTA
    AAGCCAATTT CTTTCAAAAG AGAGCATGAA GAGCGGCTTT CAGATGTAAA GTATTCTGAG
    TCTCTTGAAA AGAATAAGTT CTATGAATTT GCATTGGATA AGACGATAAC ACCAGACACA
    AAAGCTTTGC TTGAGCGTGC TAAGTCACTG TCTTCATCTA GAGAAGAAAA TTGGTTTTTC
    CTTAATCATG ACTCAAAATT CAAAAGTTTA CAGAATAACA TCGACAAGGA AAAGTCTGAA
    CCTACTCCAA GACCTATTCC TTCATGGTAC TTGAAGAAAA AGAAAAGTCG CTCTGACTCT
    GATGGAAAAC TTGATGAAAA AAAGCAAGAT GCTAAGCCTG ATGAACAAGA AAGACAAGAT
    CTCCTAGCTT CACGATTTCT TCATAGCTCA ATCTTTGAAC AGGATTCACG TCGGCTTCAA
    CACCTTGAAC GGAAAAATAC TGACCCTGAC GTTAGAATTG GCAGAGATAG TGTTACAAGT
    AATTCTCAAG GTGAACAACC TGGAACAGGA GGATCAGACT TGACTCAAGA ACCAAGAGTA
    CTTTTTCATA GCCGCTTTTT GGAACTTCAG CAAAGAGACA AGAACCAACA GCTTCCTATT
    TCAGAAAAAC TTAGTTTAAC TGATCAAATG GAGAAGACAT TGGATGAAGA GTTTAGGCCA
    TCTCCCAATT TATCAGACAT TTCCCAAGAG TCATTAGCAA GTCATAGCTT AACACCTACA
    ATATCCCCAG TGTCTCAGTC TAAAATTTCA GAGATGTCTG TGCACCATGA GAAGCCTGTT
    TTGCATACTA CTTTACCAAA CATACATAAT CCAGCAGTTG TGAATGTTAG GTCAGAAAAT
    GCTCCAATAG ATTATCCATC GTTAATTAGC CTAAAGGATG AGAACAACAC TATTCCGTTG
    AGCACTCCAT CACCTGAGCC CATGATAAAG GAAGATGTAA TTGTTGAAGA ACCTGAAGAA
    AAACTCAGAG ATTCCAGAAA AACTGTACAC GCCGCTGTTG AACACGAGAT TGAGATCAAA
    CCTCCCACTC CTGGTGCATC TTTAGGTAAT GTTGAGCCAG AATGTGATGC TCTGCAGGCA
    TCTTCTCCAT TGTATCCAAA TCCCAGTAAA GATCAAGAGA CTACAGAGCT CTCAGAAACA
    ACAACAGAGC CTGCTAATTC TTCTCTTGTT GTATCTCCTC TGGAGGTGCC AGACACAGAA
    ATTGAGCAAG TTGAACAAGT ACTACCACCG CGTAAACAAC CAAAGAGTAA AAAGGTAAAG
    AATGTCTCAT CAGCGCAAAC ACCAATCACC CAAGTCGTTA ATGAAAAACC AGCTACACGA
    AAGAGTGAAC GCATTGATAA AGAAAAGCTA AAACGATCCC CCAGAGGAGA CTCTACAAAA
    CTTACTGCTG ATTCCAGAAA TTCTGCAAAA TCTCCTGTTC AAGTTTTAGA TTCAGAGCAG
    GGCCTTGAAT TAAACACAAA CCAAGGCAGA ACACGCCAAC GACGGAATGT GCGATCTGTC
    TATGCAACAC CCCCAGAGGA AGAATCTCCT CAACAACAAG GAAAAGAGGT AACAGAACCT
    CCCCGTTCCA CTCTTAAGCG CTCTAGGGGT CGTCCACCCA AGACTCGCAG ACGAGTGGAT
    GATATGTCTC CGGTAAAAGG AGATCAGATC AAAAATTCTG AGGCTGAAGA CACTGAAAAT
    AAAGAGTCAA CAAGTAGTGG AGAAATAACT AAAAGTACAG AGGCATGGCG CTCTCCACGT
    TCTCAAAAGG GCCAGTCTTC ACCGATAAGA TCAGGACAAA GCAAAAAGAC ATTGAAATTG
    GACAAAGTGT CAGGTACTTC GGCATGTGTT CAATTAGACA GAGAAGCAGT TGATGTGCCA
    CCTGCCCTAG ATCGCAAAGC TGAATCCAAA GAAACTTTGA TGCAAGTTGG ACATTTGTCA
    AAAGATACCA AACAGAACAG CATATCTACA AGAGAAAAAG AACTTGTTGC TGAGGAAATT
    TCAACAGATG ACCGTGTAGA AATCACTCCT GTAGAAAAAA TATTAGACAA GGAGGTCAAG
    ACATCAAGGT CAACACGGAA CACAAAAACA ATGTCTGATA ATAAGGCTGT CAATGTGGTG
    AAGCTCACTA TGGATGCAGT TAAGGAAGCT GTTCATTCAG GTGATGACAT AACTGTTTGT
    TTTGAAGGGT CTTTAAAAGC TAATGTTCCA CAATCAGTAA TAGAACAGTC AGACATTCAA
    GATTATCACA AAAAGGAAAA TGTTAACAGC CTTGATGAAA AGGATGAGAC TCTTTCAGAA
    ATGGAGCCTC CTACTGATCC TGTGGCTGCC CTGCTTGCTC GCCAAATGGA ACTGGAAAGA
    GCAGTGGAAA ATATTTCAAG ATTTACAGAT GACCAGCATC CAGTACCTTA CAAAGAACCT
    CGTACAGAAC CACCTACCCT AACAGCACCT GTTGTAGTAC AACCTGCAGA GGATACAGAG
    GTAGGAAAGC CTGCCAATCC AGCAAGTGAA ACTGAATTGG CTGAAGCAAT TAATTCAATT
    ACTGCTGAGG ATATATCAGG TGATACAGAT GGATTCTCTG CTTCAACAAC ATACACAACC
    CTCCTTCCTC AACCAGAGAC TCTTGATTTG CCTGTACCAA ATGAGGTTGT GGAAACTGAG
    TCAGACTTGA CTGTAAAGAC TATTGCACAG TCTGAACAAG ATAATGTTAT GGTTCCAGTT
    TCAAAATCTT CTAAATGTAA AACAGATACT TCACTCAAAG AATCACCATT GACAGAGGTA
    GCAAAAAGAG GAGGAAGAGC ACGTCCAAAA ACTGCAAAAA AATCCAGAGG GAGGAAGGTT
    TCTTTGAACA GAAAATTAGA TATTGCTGAG GATGTAGTGC TGGAACCTGA ATCCACTACA
    GTCAAACTTC CAGAATCGAT TCCAGAAGAG ATTCAAACAG TTAACCCTAA GGCAGCAACA
    TCGGCAGCTG CAGCAGCTGT AGTCACAGTT GCTGCTGCAT GCAAACATGA AGCTGCATCT
    ACAGTGATTT TGAATACACC AAAAGAGGCA GAACAGCCTG CAGTTGATCA GCCTGAACCT
    CAAGAATCTG CCTTCCATTC TGGAAATAAC AGTCCTTCTT ATTTGAGGAC ACAACAGTCA
    TCTCCTGAGC GAGGAGCATC TGCCCTTACT TCACCTACAA CACGCCTAAA TTCACCTGCA
    TCCTCTAACA TTCCTCCTGA ATGGAATACC AGGACAGAGG AGAAAGTAAT GCTACCTAAA
    CCACAAGGTA ATGTGGCTTT GTCTATCCCA GGAGCTGGGG GACCCCCAGC AAATCCTCCC
    ATGCCTCCTG ACACAAAAGC CTCTGATATT AATGCAAGTT CTAGTACACT GCGAAAAATT
    TTAATGGAAC CAAAGTATGT TTCTGCTTCA AATAGCAATT CAGTGACAAG TATGCAGTTT
    ACTACCACAT TAGCAGATCC ACGGATGTCT GATAATGAAA ATTCAGTTGA GGCTGTGCTG
    CCTTTAAAAA CCTCTCTACC TGAAGATAGA CCTAGCCCTA TAGCTCAGCT TGTACCCCGT
    ACAACACCAC CGCAGCCCCC TCCTCTACAA CAATGTGAGA CTCCACAGAT CTTAAAGGAG
    AAACTAGCTA TAACTTCCAC TGCCACTTCA GTCATTAGTC GGATCCCTAT GCCCTTTGAT
    TTTGAGGACA CTCCTCGCAT CTCTTTAAGT AATCGTAACT CTGGAATGTC TTTGCCAAAG
    CAGAAATATC GTACTGGCTT GAATGAAAAT AATAGATATC ATGGACATAA TACTTCTGAG
    GATGGTGGAA GTGTTGGGCG CCCAGTTGTT GAAGGCACAC ATTGTAACAC GGGGTCTAGT
    ACTGGTTTGA GGGTCAACAC ATCTGAGGGT GTGGTAGTAT TAAGTTATTC TGGACAAAAG
    ACAGAGGGAC CACAGCGGAT CATTGCAAAA ATTAGCCAAA TCCCTCCAGC CAGTGCAGTT
    GACATTGAAT TTCAGCAGTC TATAACAAAA TCTCAGATTA AACAAGAACC TTCATCTCAC
    CCCTCTACCC CAAAAGGATC ACAGACACCT ACAGGCTATA GTCACACAGG GGTAGTATTA
    GCTGGCCAAA GTATCAATGC CCAACCTGTT ATATCCTCAA TACGCCAGGA AAGCCCTGGC
    TCTGAAAAAT CAGAAACATC TTACCAACAA GGAAGTTCTC TGAAATCTTT CCACCAGTCC
    CCAAGCCATC CTCAACTTCT GAGGTATGGT CAGTCAATAA CACCGCAACA CCATATGAAG
    AAAAATGGAG AAACTGAATC ATTTTCAATG AAAGCTGATA TAAAACCTGA TATAAAGGCA
    ACACAGACTT TCAATGTTAA ACCAGTTTTA AGTCCACGCC ACCCATCTTT CTCAGGCAAT
    CACATTTTAA GTCCAAGTGC TCCACATGAA AGAGCTGTCC CACAACCCAA GCAAGATTCA
    CATTCTCCAA GGGCATCTAA CCATTCCATG TCACCCTTTC CAAAGGTTTG CCCCCCTAAC
    AGCCCCGTTG TTCTGGGACC AGCTGGCCCT ATGTCTCAGT ATGTGTCAAA TATACACCAT
    GCTGAGCAGT CAGTGATCAT GCCTCCTCAT AGTGTCACCC AGTCTGTGCC CATGAGTCAT
    TTGTCCCAAG GGGATGTCCG AGCCAGTACT CCAACATTAT CTGGAATAGG TTATGGATTG
    CGTTCAGAAA ATCTCTTATC ACCTCGATCG GCACCTCCTC AACGTTCCAC GACTCCTCAA
    CCTGCAGTGA TCAGAGATGT CATATTGAAA TCGCATGCAG GCTCGTCTGT TGGTGGCCAT
    GTTGTTGAGA CGAGCAATGA TGATGCACAG AATCTCCCAC AAGGACTTCG TAGATCTTCT
    GCCCCACAGT TGCAACAGGA AAGTATGGTA ATACAGTCGG AATTCAAAAA TCTGCATCAC
    AGGGCATTAC GTCTAGATCA GTATGCCAGA CTTATGCAGC AGCATTTGAC TGACCATCCA
    GGTGTTGTTG AAAGTCGCCA AACTCGAACT GCAGAGGTGG TGCAGTCATC TTCACACATA
    TCATCTGCTT CTTCTAAAGC CTCTTCCATA GTAAAGAATG CTCCACAGAT TGTAAGGGAT
    GGACAAAAAA CAGTGGAGCT GAAGATGTCA CCCTCCCCTC ATTCAGACAA CAGGATAACA
    GGACACCCAC CAGGCTCAGT GATGGTATCT CCTCAAGGAG TTCAGTTAAA TTACCCTGGA
    AATGGAAATA CCATGAATGA ATACTACAAA GAAATGCGTG GCTTCCATCC TCAGTATCCA
    GGTCATTCAG TAATTGGAAT TAACTTGGCT AACCGTGGGA TTCCTGTGTC TCAGGTCTCA
    CAAATTGATC ACAGTCAAAG ACACAAGGTT CCCTCTCTCT CTTCCAGTGA GTCTGTGGGA
    AGTTTTAGTG AGTCAAAACT TGAGGGTTCT CACATTCGAC ACTCAGGCAC CATGGATTTG
    TCTCATATAT CACGAGTTCA GAGTGAGGCT GGGTCCCCCT CTTATATTTC TCCAGTGACT
    ATAACACCCA AACTGGAGTT GGCTATTACT TTGCAGAAGG GGCCTCAAGG ACCTGTTTCC
    AACAAGATGC CACTTCCTTC TTTAGCTTCT TCACAGATGC GTTCAGACTT CAAACTTGAC
    CACACAGGAC TTCGGTCTGT TGATATGGTG CAGTTGTTAA CGAAGTACCC AATCATTTGG
    CAAGGTCACC TGGCACTTAA GAATGACACT GCAGCAGTGC AGCTGCACTT TGTGTCTGGT
    AATAATGTTC TGGCTCATCG TTCACTGCCA CCCCCAGAAG GAGGTGCCTT TCTTCGCATT
    GCTCAGAGAA TGCGCTTAGA GGCTTCACAA CTGGAAGGAG TTGCGAGACG CATGACTGCT
    GAAAATGAAT ACTGCCTTCT GTTGGCATTG CCTTGTGGAT TGGACCAAGA AGATGTCCAC
    AACCAGACCC ATGCTCTTAA AACTGGTTTT ATTACTTACC TGCAAGCAAA ACAGGCTGCT
    GGAATTATCA ATGTCCCAAA CCCAGGCTCA AATCAGCCTG CCTACGTTGT CCAGATTTTC
    CCTCCTTGTG AGTTTTCTGA GAGCCACCTG TCTCATCTTG CTCCGGACCT TCTCAACAGC
    ATCTCCAGTA TCTCTCCTCA TCTTATGATT GTTATAGCTT CTGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41575
    Clone ID Related Accession (Same CDS sequence) XM_009296917.3 , XM_009296917.2
    Accession Version XM_009296917.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 10428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product msx2-interacting protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    ATGGTCCGGG AAACCAGACA TTTATGGGTG GGAAATTTAC CAGAAAATGT ACGAGAGGAA 
    AAAATTATCG AGCACTTCAA ACGGTATGGA CGAGTGGAGA GTGTCAAGGT TCTCCCTAAA
    CGTGGATCGG AAGGTGGAGT GGCAGCTTTT GTGGATTTTG TGGACATTAA GAGTGCTCAG
    AAAGCTCATA ATTCTATCAA CAAGATGGGA GACAGGGACC TTCGCACCGA TTACAATGAA
    CCAGGAACTA TACCCAGTGC AGCTCGAGGC CTGGATGATA GCCTGTCCAT AGCCACTCGT
    GGGCGGGATG TTTCAGGGTT CACAAGGGGG GCAGGGGGCC CTGTGTATGG GCCCTCTGTG
    TCACTTCACA GCAGAGAGGG ACGCTTTGAA CGCAGACTAG ACGGGACTTC GGACAGCCGG
    GAACGCTCTT ACGATCACAG TGCCTATGGA CACCATGAGC GTAGCAGCAG CAACAGCACT
    AGTTTCGATC GGCAGCGTCA CTATGAGAGC GATTATTACA GAGACTCTAG GGACCGGGCC
    CTCAGTGGAA CCAGTGGGAG TGCCAGTTCA GCCAGTGGAA GCATCGGAGG AAGCTCATCT
    GCGGGCAGTA TAGGTGTTGC AGGGAATGTT GCAGGAAGCA GTGGTGCTGG TGGAAGTACT
    GCCGCTGGAG TAGGCAGTGG TGGCTCCACA CCAGGAGGAA TTGTCTATTA TGGTTCACGA
    AGCCGGAGCC CCAGTCGTTT TGAGACTACA GAGACTCGTT ATGAGCCCCG TGCCCGTGAA
    GCTTTCACAC TTGCCAGTGT GGTTCATCGG GATTTATTCA GAGAAGAAAG AGGCAGACGT
    GGGGAAAGGA CATACCACCA CAGTCGCAGT AGATCACCAC ACTCTTCACA GTCACACAAT
    CCATCACCAC AGAGGTTGCA AAGTCAGGCA GCGCGACCGG CACGCTCCCG CAGTGGCTCA
    GGATCCCGCA GTCGCTCCTC CAGCTCAGAC TCCGTCAGCA GTACCAGCAG CAGTGGCAGT
    GGCAGTGATT CCAGCAGCAC CTCCAGTGAC CGATCTCCAG CTCGATCTGT TCAGTCCACT
    GCAGTTCCTG CCCCTTCTAC ACAGCCACTA CCTTCACTGG ACAAAGATGA ACCTCGTAAA
    AGTTTTGGTA TCAAAGTGCA GAACCTGCCC GTGCGCTCAA CAGATACAAG CCTCAAGGAT
    GGTCTTTTCC ATGAATTTAA GAAACATGGT AAAGTCACAT CAGTTCAGAT CCACGGTGCC
    TCTGAGGAAC GATATGGCTT AGTGTTCTTT CGCCAGCAAG AGGACCAAGA AAAAGCTCTA
    AGTGCATCTA AAGGGAAGCT GTTTTTTGGC ATGCAAATTG ATGTCACTGC TTGGCATGGC
    CCAGAAACAG AAAGTGAGAA CGAGTTCCGG CCTTTGGATG AACGCATAGA TGAGTTTCAT
    CCCAAAGCTA CAAGGACATT GTTCATAGGA AATTTGGAAA AAACCACAAC CTATAATGAC
    CTGCTGAACA TATTTCAGCG TTTTGGAGAG ATTGTTGATA TTGATATTAA AAAGGTAAAT
    GGTTCCCCAC AATATGCCTT TCTCCAGTAT TGTGACATAG CAAGTGTCTG CAAAGCAATT
    AAGAAAATGG ATGGTGAATA TCTTGGCAAC AACAGGTTAA AGCTTGGTTT TGGCAAGAGT
    ATGCCTACTA CATGTGTATG GTTGGATGGC TTGTCCTCCA GTATTACTGA ACAGTATCTC
    ACGAGGCACT TTTGTCGTTA TGGACATGTA GTAAAGGTTG TCTTTGACCG GCTGAAAGGA
    ATGGCCCTCA TCTTATATAA TAATATAGAG TATGCGCAGG CTGCTGTCAA GGAAACAAAG
    GGTTGGAAAA TTGGAGGAAA TAAAATAAAG GTTGACTTTG CTAACCAGGA AAGTCAGATG
    GCTTTCTACC GTTCAATGCA GGGATCTGGA CAAGACATAA GAGATTTCTA TGAAATCATA
    TCTGAACGAA GAGATGAGCG CCGGACGCCA TATCACGAGT TCACAGCTGA ACGTGCATAC
    TATGAAAATG TGCGTACCCC AACTTCTTAC ACAGAGGATC CACGTCGAAA ATATCCTGCC
    AGAAGTCGAG AGTTTTTTTC AGAATGGGAT CCCTACCAGG GAGATTACTA TGACCCACGA
    TATTATGATG ACTCCCGTGA ATACAGGGAT TTTAGAGACC CATATGAGCA GGACATTAGA
    AAGTACAGCT ACTTGCAAAG AGAACGTGAA AGGGAAAGAG AGAGGTTTGA AACAGATCGT
    GAGCGAGACC ATGGTCGACG AACAATTGAA CACCAACAAA GCCCAACCCA TTCACGTCGT
    CCTGCTAGTC CAACTGCTTC TCCATCCCTT TCTGAACGTC CTCCCAGTGA CTCTGAACAT
    CACGTTTACA GTCGCTCATC TGAGCGAAGT GGTAGCTGCA GCTCACTTTC TCCACCACGC
    TTTGAGAAGC CAGACAAAAT TCGTTTGGAG AGGCACAATA GGAATGATAA ATTGGAGAAA
    GAAAAATCAC TTTTTGAGAC AGAGCGTGGA AATGGTGGGG AAAAAGAAAG GCGAGCAGGA
    CGTAAGGAAA AAGGAGAAAA GGACAGGACT GAGAGACAGA AGTTGAAGAA GTTAAAACTT
    GCATCTCCCA CTGTTCCATC ACCAGAGGCA GAGCTGGAAC TTGACAGGGA TGCAAGCCCA
    GAGGCTAACA TAACTCTGCG AGGTAAAGTG AGCAAATCAT TGATCAAAGA CAAAGACTAC
    TCTGGTAAAG GAAAACTTGA TCTGCTACCT TGTGTTGTCC AGCTAACAAG AGTAAAAGAG
    AAGGAAGGAA AATTAATAGA CAGTATCCTC TGTGAAAAAC AAAAAACAAA GGTTGGAAGT
    GATCCTGTTC TGTCTCCAAC AACACCTTCC TCAGGTGATC ACAAGAGTAT GCCTTTTCGC
    AAAGACACTC AGGCTAGAGA TTTCTTTAAG CATGGAAAAC ATCTCAAGGA GAAAGGCCTG
    GCAAGCCAAG TTGAGGTAGT GGATAAAGAG GAAAAAGTGA AAAACAAAAA ATATTTCAAA
    TCTGATCTTG CATTTGAATC CAGCTCTTCT GTGGATGCTG ACCGCAAGGC TGCACGAAAG
    AGACGCTTTG AAGAAACATC GGCTAAGGCT GATAATTTAA GGAGAGTAAG TCAGGAGGAG
    GATGAAGGGA AATTAAGGAG AATTATTGAT GAACCTTTGC TGAAAGACAC TGATTATGAT
    AAAAAGTTGC TTCGGAAAGA AGCACACAAG AGAGAGCGAA AAATTAAACC AGAGAGGATG
    GTTACTGTTA GTACTACAAT AGAAGAGTTA GACAATGTAA TGCCAGTAGG CCCCAGTTTA
    GACCTTCAGG CTCGACTTGG GGAACCAGCT GAAGATGCAA TAGATCCATT AGATAGCCTT
    GATCAAAAGA TAGGTGCTTT TGGAGCTAAC AGACAACCAA GTTTCAGTCT TGCAGTCTCC
    GACGATGGAA GTGTAGATAT GGATTTTTCA AGAGAGCAAG AGCAGCAGCA TTTAACTAGC
    TACCACACAT TATCCTCAAG GCAAGAAAGA GTTTCTGAAA GTAAGGAAAG TTTGCTCTCA
    GACATTGATC ATTCGCAGAG TTGCAGAAAA CAAATGGAGC AAAATCGCCG CTTACAGCAG
    CAAATGCTGG AGTGTGATAA ATCAGATAAA ACTGAAAGCA CACCAAGCAC TGATGCGGAG
    GAATTTGAGC ATCGAAGTAT TGTGCATGAG GTTGTAAAAC CATTACAAGA TGTAACGGGA
    AGTTCTCCGA CCAGCAAGCA AAAAAAATTG GGAGGATTCG AATTTGATTT TGGCAAAAGA
    GAGCAAAATT ATGAAATGTT TCGACTGAGA AATGATGAAC CTGAAAGAGG ACTTGCTTCA
    CATCCAGGGA CACCCCTTGC TGAGGAGGAG AGAAATGCAT CACAATTGTT GGACAAAGAC
    CCAGATTTAC CAGTGACAAT GGATAAAAAT AGCTCGCATT TAGATGTTTC AAAATACAAT
    ACTAGCAATG CAGTGCATCA AGGGCTTTCA CCACATGCAG AGATACTAAA AGTGAAACCA
    TCAGTTACTA AAGAGGAGTT CAGTTGGGAG AGCAATATTA GGCAGGGCAC ATTGAGAGAA
    ATGAGCTTTC CCACAAGCAT TGTTAAACGG GAAAGTATCA GAAAACGTCC TGAACGCGAA
    TTAGAACCTG GAGAGGTTCA GTCAGACTCT GATGAAGATG AAAGCAGACA CTATTCATTA
    AAGCCAATTT CTTTCAAAAG AGAGCATGAA GAGCGGCTTT CAGATGTAAA GTATTCTGAG
    TCTCTTGAAA AGAATAAGTT CTATGAATTT GCATTGGATA AGACGATAAC ACCAGACACA
    AAAGCTTTGC TTGAGCGTGC TAAGTCACTG TCTTCATCTA GAGAAGAAAA TTGGTTTTTC
    CTTAATCATG ACTCAAAATT CAAAAGTTTA CAGAATAACA TCGACAAGGA AAAGTCTGAA
    CCTACTCCAA GACCTATTCC TTCATGGTAC TTGAAGAAAA AGAAAAGTCG CTCTGACTCT
    GATGGAAAAC TTGATGAAAA AAAGCAAGAT GCTAAGCCTG ATGAACAAGA AAGACAAGAT
    CTCCTAGCTT CACGATTTCT TCATAGCTCA ATCTTTGAAC AGGATTCACG TCGGCTTCAA
    CACCTTGAAC GGAAAAATAC TGACCCTGAC GTTAGAATTG GCAGAGATAG TGTTACAAGT
    AATTCTCAAG GTGAACAACC TGGAACAGGA GGATCAGACT TGACTCAAGA ACCAAGAGTA
    CTTTTTCATA GCCGCTTTTT GGAACTTCAG CAAAGAGACA AGAACCAACA GCTTCCTATT
    TCAGAAAAAC TTAGTTTAAC TGATCAAATG GAGAAGACAT TGGATGAAGA GTTTAGGCCA
    TCTCCCAATT TATCAGACAT TTCCCAAGAG TCATTAGCAA GTCATAGCTT AACACCTACA
    ATATCCCCAG TGTCTCAGTC TAAAATTTCA GAGATGTCTG TGCACCATGA GAAGCCTGTT
    TTGCATACTA CTTTACCAAA CATACATAAT CCAGCAGTTG TGAATGTTAG GTCAGAAAAT
    GCTCCAATAG ATTATCCATC GTTAATTAGC CTAAAGGATG AGAACAACAC TATTCCGTTG
    AGCACTCCAT CACCTGAGCC CATGATAAAG GAAGATGTAA TTGTTGAAGA ACCTGAAGAA
    AAACTCAGAG ATTCCAGAAA AACTGTACAC GCCGCTGTTG AACACGAGAT TGAGATCAAA
    CCTCCCACTC CTGGTGCATC TTTAGGTAAT GTTGAGCCAG AATGTGATGC TCTGCAGGCA
    TCTTCTCCAT TGTATCCAAA TCCCAGTAAA GATCAAGAGA CTACAGAGCT CTCAGAAACA
    ACAACAGAGC CTGCTAATTC TTCTCTTGTT GTATCTCCTC TGGAGGTGCC AGACACAGAA
    ATTGAGCAAG TTGAACAAGT ACTACCACCG CGTAAACAAC CAAAGAGTAA AAAGGTAAAG
    AATGTCTCAT CAGCGCAAAC ACCAATCACC CAAGTCGTTA ATGAAAAACC AGCTACACGA
    AAGAGTGAAC GCATTGATAA AGAAAAGCTA AAACGATCCC CCAGAGGAGA CTCTACAAAA
    CTTACTGCTG ATTCCAGAAA TTCTGCAAAA TCTCCTGTTC AAGTTTTAGA TTCAGAGCAG
    GGCCTTGAAT TAAACACAAA CCAAGGCAGA ACACGCCAAC GACGGAATGT GCGATCTGTC
    TATGCAACAC CCCCAGAGGA AGAATCTCCT CAACAACAAG GAAAAGAGGT AACAGAACCT
    CCCCGTTCCA CTCTTAAGCG CTCTAGGGGT CGTCCACCCA AGACTCGCAG ACGAGTGGAT
    GATATGTCTC CGGTAAAAGG AGATCAGATC AAAAATTCTG AGGCTGAAGA CACTGAAAAT
    AAAGAGTCAA CAAGTAGTGG AGAAATAACT AAAAGTACAG AGGCATGGCG CTCTCCACGT
    TCTCAAAAGG GCCAGTCTTC ACCGATAAGA TCAGGACAAA GCAAAAAGAC ATTGAAATTG
    GACAAAGTGT CAGGTACTTC GGCATGTGTT CAATTAGACA GAGAAGCAGT TGATGTGCCA
    CCTGCCCTAG ATCGCAAAGC TGAATCCAAA GAAACTTTGA TGCAAGTTGG ACATTTGTCA
    AAAGATACCA AACAGAACAG CATATCTACA AGAGAAAAAG AACTTGTTGC TGAGGAAATT
    TCAACAGATG ACCGTGTAGA AATCACTCCT GTAGAAAAAA TATTAGACAA GGAGGTCAAG
    ACATCAAGGT CAACACGGAA CACAAAAACA ATGTCTGATA ATAAGGCTGT CAATGTGGTG
    AAGCTCACTA TGGATGCAGT TAAGGAAGCT GTTCATTCAG GTGATGACAT AACTGTTTGT
    TTTGAAGGGT CTTTAAAAGC TAATGTTCCA CAATCAGTAA TAGAACAGTC AGACATTCAA
    GATTATCACA AAAAGGAAAA TGTTAACAGC CTTGATGAAA AGGATGAGAC TCTTTCAGAA
    ATGGAGCCTC CTACTGATCC TGTGGCTGCC CTGCTTGCTC GCCAAATGGA ACTGGAAAGA
    GCAGTGGAAA ATATTTCAAG ATTTACAGAT GACCAGCATC CAGTACCTTA CAAAGAACCT
    CGTACAGAAC CACCTACCCT AACAGCACCT GTTGTAGTAC AACCTGCAGA GGATACAGAG
    GTAGGAAAGC CTGCCAATCC AGCAAGTGAA ACTGAATTGG CTGAAGCAAT TAATTCAATT
    ACTGCTGAGG ATATATCAGG TGATACAGAT GGATTCTCTG CTTCAACAAC ATACACAACC
    CTCCTTCCTC AACCAGAGAC TCTTGATTTG CCTGTACCAA ATGAGGTTGT GGAAACTGAG
    TCAGACTTGA CTGTAAAGAC TATTGCACAG TCTGAACAAG ATAATGTTAT GGTTCCAGTT
    TCAAAATCTT CTAAATGTAA AACAGATACT TCACTCAAAG AATCACCATT GACAGAGGTA
    GCAAAAAGAG GAGGAAGAGC ACGTCCAAAA ACTGCAAAAA AATCCAGAGG GAGGAAGGTT
    TCTTTGAACA GAAAATTAGA TATTGCTGAG GATGTAGTGC TGGAACCTGA ATCCACTACA
    GTCAAACTTC CAGAATCGAT TCCAGAAGAG ATTCAAACAG TTAACCCTAA GGCAGCAACA
    TCGGCAGCTG CAGCAGCTGT AGTCACAGTT GCTGCTGCAT GCAAACATGA AGCTGCATCT
    ACAGTGATTT TGAATACACC AAAAGAGGCA GAACAGCCTG CAGTTGATCA GCCTGAACCT
    CAAGAATCTG CCTTCCATTC TGGAAATAAC AGTCCTTCTT ATTTGAGGAC ACAACAGTCA
    TCTCCTGAGC GAGGAGCATC TGCCCTTACT TCACCTACAA CACGCCTAAA TTCACCTGCA
    TCCTCTAACA TTCCTCCTGA ATGGAATACC AGGACAGAGG AGAAAGTAAT GCTACCTAAA
    CCACAAGGTA ATGTGGCTTT GTCTATCCCA GGAGCTGGGG GACCCCCAGC AAATCCTCCC
    ATGCCTCCTG ACACAAAAGC CTCTGATATT AATGCAAGTT CTAGTACACT GCGAAAAATT
    TTAATGGAAC CAAAGTATGT TTCTGCTTCA AATAGCAATT CAGTGACAAG TATGCAGTTT
    ACTACCACAT TAGCAGATCC ACGGATGTCT GATAATGAAA ATTCAGTTGA GGCTGTGCTG
    CCTTTAAAAA CCTCTCTACC TGAAGATAGA CCTAGCCCTA TAGCTCAGCT TGTACCCCGT
    ACAACACCAC CGCAGCCCCC TCCTCTACAA CAATGTGAGA CTCCACAGAT CTTAAAGGAG
    AAACTAGCTA TAACTTCCAC TGCCACTTCA GTCATTAGTC GGATCCCTAT GCCCTTTGAT
    TTTGAGGACA CTCCTCGCAT CTCTTTAAGT AATCGTAACT CTGGAATGTC TTTGCCAAAG
    CAGAAATATC GTACTGGCTT GAATGAAAAT AATAGATATC ATGGACATAA TACTTCTGAG
    GATGGTGGAA GTGTTGGGCG CCCAGTTGTT GAAGGCACAC ATTGTAACAC GGGGTCTAGT
    ACTGGTTTGA GGGTCAACAC ATCTGAGGGT GTGGTAGTAT TAAGTTATTC TGGACAAAAG
    ACAGAGGGAC CACAGCGGAT CATTGCAAAA ATTAGCCAAA TCCCTCCAGC CAGTGCAGTT
    GACATTGAAT TTCAGCAGTC TATAACAAAA TCTCAGATTA AACAAGAACC TTCATCTCAC
    CCCTCTACCC CAAAAGGATC ACAGACACCT ACAGGCTATA GTCACACAGG GGTAGTATTA
    GCTGGCCAAA GTATCAATGC CCAACCTGTT ATATCCTCAA TACGCCAGGA AAGCCCTGGC
    TCTGAAAAAT CAGAAACATC TTACCAACAA GGAAGTTCTC TGAAATCTTT CCACCAGTCC
    CCAAGCCATC CTCAACTTCT GAGGTATGGT CAGTCAATAA CACCGCAACA CCATATGAAG
    AAAAATGGAG AAACTGAATC ATTTTCAATG AAAGCTGATA TAAAACCTGA TATAAAGGCA
    ACACAGACTT TCAATGTTAA ACCAGTTTTA AGTCCACGCC ACCCATCTTT CTCAGGCAAT
    CACATTTTAA GTCCAAGTGC TCCACATGAA AGAGCTGTCC CACAACCCAA GCAAGATTCA
    CATTCTCCAA GGGCATCTAA CCATTCCATG TCACCCTTTC CAAAGGTTTG CCCCCCTAAC
    AGCCCCGTTG TTCTGGGACC AGCTGGCCCT ATGTCTCAGT ATGTGTCAAA TATACACCAT
    GCTGAGCAGT CAGTGATCAT GCCTCCTCAT AGTGTCACCC AGTCTGTGCC CATGAGTCAT
    TTGTCCCAAG GGGATGTCCG AGCCAGTACT CCAACATTAT CTGGAATAGG TTATGGATTG
    CGTTCAGAAA ATCTCTTATC ACCTCGATCG GCACCTCCTC AACGTTCCAC GACTCCTCAA
    CCTGCAGTGA TCAGAGATGT CATATTGAAA TCGCATGCAG GCTCGTCTGT TGGTGGCCAT
    GTTGTTGAGA CGAGCAATGA TGATGCACAG AATCTCCCAC AAGGACTTCG TAGATCTTCT
    GCCCCACAGT TGCAACAGGA AAGTATGGTA ATACAGTCGG AATTCAAAAA TCTGCATCAC
    AGGGCATTAC GTCTAGATCA GTATGCCAGA CTTATGCAGC AGCATTTGAC TGACCATCCA
    GGTGTTGTTG AAAGTCGCCA AACTCGAACT GCAGAGGTGG TGCAGTCATC TTCACACATA
    TCATCTGCTT CTTCTAAAGC CTCTTCCATA GTAAAGAATG CTCCACAGAT TGTAAGGGAT
    GGACAAAAAA CAGTGGAGCT GAAGATGTCA CCCTCCCCTC ATTCAGACAA CAGGATAACA
    GGACACCCAC CAGGCTCAGT GATGGTATCT CCTCAAGGAG TTCAGTTAAA TTACCCTGGA
    AATGGAAATA CCATGAATGA ATACTACAAA GAAATGCGTG GCTTCCATCC TCAGTATCCA
    GGTCATTCAG TAATTGGAAT TAACTTGGCT AACCGTGGGA TTCCTGTGTC TCAGGTCTCA
    CAAATTGATC ACAGTCAAAG ACACAAGGTT CCCTCTCTCT CTTCCAGTGA GTCTGTGGGA
    AGTTTTAGTG AGTCAAAACT TGAGGGTTCT CACATTCGAC ACTCAGGCAC CATGGATTTG
    TCTCATATAT CACGAGTTCA GAGTGAGGCT GGGTCCCCCT CTTATATTTC TCCAGTGACT
    ATAACACCCA AACTGGAGTT GGCTATTACT TTGCAGAAGG GGCCTCAAGG ACCTGTTTCC
    AACAAGATGC CACTTCCTTC TTTAGCTTCT TCACAGATGC GTTCAGACTT CAAACTTGAC
    CACACAGGAC TTCGGTCTGT TGATATGGTG CAGTTGTTAA CGAAGTACCC AATCATTTGG
    CAAGGTCACC TGGCACTTAA GAATGACACT GCAGCAGTGC AGCTGCACTT TGTGTCTGGT
    AATAATGTTC TGGCTCATCG TTCACTGCCA CCCCCAGAAG GAGGTGCCTT TCTTCGCATT
    GCTCAGAGAA TGCGCTTAGA GGCTTCACAA CTGGAAGGAG TTGCGAGACG CATGACTGCT
    GAAAATGAAT ACTGCCTTCT GTTGGCATTG CCTTGTGGAT TGGACCAAGA AGATGTCCAC
    AACCAGACCC ATGCTCTTAA AACTGGTTTT ATTACTTACC TGCAAGCAAA ACAGGCTGCT
    GGAATTATCA ATGTCCCAAA CCCAGGCTCA AATCAGCCTG CCTACGTTGT CCAGATTTTC
    CCTCCTTGTG AGTTTTCTGA GAGCCACCTG TCTCATCTTG CTCCGGACCT TCTCAACAGC
    ATCTCCAGTA TCTCTCCTCA TCTTATGATT GTTATAGCTT CTGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295192.1
    CDS2251..12678
    Translation

    Target ORF information:

    RefSeq Version XM_009296917.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spen family transcriptional repressor (spen), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296917.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    ATGGTCCGGG AAACCAGACA TTTATGGGTG GGAAATTTAC CAGAAAATGT ACGAGAGGAA 
    AAAATTATCG AGCACTTCAA ACGGTATGGA CGAGTGGAGA GTGTCAAGGT TCTCCCTAAA
    CGTGGATCGG AAGGTGGAGT GGCAGCTTTT GTGGATTTTG TGGACATTAA GAGTGCTCAG
    AAAGCTCATA ATTCTATCAA CAAGATGGGA GACAGGGACC TTCGCACCGA TTACAATGAA
    CCAGGAACTA TACCCAGTGC AGCTCGAGGC CTGGATGATA GCCTGTCCAT AGCCACTCGT
    GGGCGGGATG TTTCAGGGTT CACAAGGGGG GCAGGGGGCC CTGTGTATGG GCCCTCTGTG
    TCACTTCACA GCAGAGAGGG ACGCTTTGAA CGCAGACTAG ACGGGACTTC GGACAGCCGG
    GAACGCTCTT ACGATCACAG TGCCTATGGA CACCATGAGC GTAGCAGCAG CAACAGCACT
    AGTTTCGATC GGCAGCGTCA CTATGAGAGC GATTATTACA GAGACTCTAG GGACCGGGCC
    CTCAGTGGAA CCAGTGGGAG TGCCAGTTCA GCCAGTGGAA GCATCGGAGG AAGCTCATCT
    GCGGGCAGTA TAGGTGTTGC AGGGAATGTT GCAGGAAGCA GTGGTGCTGG TGGAAGTACT
    GCCGCTGGAG TAGGCAGTGG TGGCTCCACA CCAGGAGGAA TTGTCTATTA TGGTTCACGA
    AGCCGGAGCC CCAGTCGTTT TGAGACTACA GAGACTCGTT ATGAGCCCCG TGCCCGTGAA
    GCTTTCACAC TTGCCAGTGT GGTTCATCGG GATTTATTCA GAGAAGAAAG AGGCAGACGT
    GGGGAAAGGA CATACCACCA CAGTCGCAGT AGATCACCAC ACTCTTCACA GTCACACAAT
    CCATCACCAC AGAGGTTGCA AAGTCAGGCA GCGCGACCGG CACGCTCCCG CAGTGGCTCA
    GGATCCCGCA GTCGCTCCTC CAGCTCAGAC TCCGTCAGCA GTACCAGCAG CAGTGGCAGT
    GGCAGTGATT CCAGCAGCAC CTCCAGTGAC CGATCTCCAG CTCGATCTGT TCAGTCCACT
    GCAGTTCCTG CCCCTTCTAC ACAGCCACTA CCTTCACTGG ACAAAGATGA ACCTCGTAAA
    AGTTTTGGTA TCAAAGTGCA GAACCTGCCC GTGCGCTCAA CAGATACAAG CCTCAAGGAT
    GGTCTTTTCC ATGAATTTAA GAAACATGGT AAAGTCACAT CAGTTCAGAT CCACGGTGCC
    TCTGAGGAAC GATATGGCTT AGTGTTCTTT CGCCAGCAAG AGGACCAAGA AAAAGCTCTA
    AGTGCATCTA AAGGGAAGCT GTTTTTTGGC ATGCAAATTG ATGTCACTGC TTGGCATGGC
    CCAGAAACAG AAAGTGAGAA CGAGTTCCGG CCTTTGGATG AACGCATAGA TGAGTTTCAT
    CCCAAAGCTA CAAGGACATT GTTCATAGGA AATTTGGAAA AAACCACAAC CTATAATGAC
    CTGCTGAACA TATTTCAGCG TTTTGGAGAG ATTGTTGATA TTGATATTAA AAAGGTAAAT
    GGTTCCCCAC AATATGCCTT TCTCCAGTAT TGTGACATAG CAAGTGTCTG CAAAGCAATT
    AAGAAAATGG ATGGTGAATA TCTTGGCAAC AACAGGTTAA AGCTTGGTTT TGGCAAGAGT
    ATGCCTACTA CATGTGTATG GTTGGATGGC TTGTCCTCCA GTATTACTGA ACAGTATCTC
    ACGAGGCACT TTTGTCGTTA TGGACATGTA GTAAAGGTTG TCTTTGACCG GCTGAAAGGA
    ATGGCCCTCA TCTTATATAA TAATATAGAG TATGCGCAGG CTGCTGTCAA GGAAACAAAG
    GGTTGGAAAA TTGGAGGAAA TAAAATAAAG GTTGACTTTG CTAACCAGGA AAGTCAGATG
    GCTTTCTACC GTTCAATGCA GGGATCTGGA CAAGACATAA GAGATTTCTA TGAAATCATA
    TCTGAACGAA GAGATGAGCG CCGGACGCCA TATCACGAGT TCACAGCTGA ACGTGCATAC
    TATGAAAATG TGCGTACCCC AACTTCTTAC ACAGAGGATC CACGTCGAAA ATATCCTGCC
    AGAAGTCGAG AGTTTTTTTC AGAATGGGAT CCCTACCAGG GAGATTACTA TGACCCACGA
    TATTATGATG ACTCCCGTGA ATACAGGGAT TTTAGAGACC CATATGAGCA GGACATTAGA
    AAGTACAGCT ACTTGCAAAG AGAACGTGAA AGGGAAAGAG AGAGGTTTGA AACAGATCGT
    GAGCGAGACC ATGGTCGACG AACAATTGAA CACCAACAAA GCCCAACCCA TTCACGTCGT
    CCTGCTAGTC CAACTGCTTC TCCATCCCTT TCTGAACGTC CTCCCAGTGA CTCTGAACAT
    CACGTTTACA GTCGCTCATC TGAGCGAAGT GGTAGCTGCA GCTCACTTTC TCCACCACGC
    TTTGAGAAGC CAGACAAAAT TCGTTTGGAG AGGCACAATA GGAATGATAA ATTGGAGAAA
    GAAAAATCAC TTTTTGAGAC AGAGCGTGGA AATGGTGGGG AAAAAGAAAG GCGAGCAGGA
    CGTAAGGAAA AAGGAGAAAA GGACAGGACT GAGAGACAGA AGTTGAAGAA GTTAAAACTT
    GCATCTCCCA CTGTTCCATC ACCAGAGGCA GAGCTGGAAC TTGACAGGGA TGCAAGCCCA
    GAGGCTAACA TAACTCTGCG AGGTAAAGTG AGCAAATCAT TGATCAAAGA CAAAGACTAC
    TCTGGTAAAG GAAAACTTGA TCTGCTACCT TGTGTTGTCC AGCTAACAAG AGTAAAAGAG
    AAGGAAGGAA AATTAATAGA CAGTATCCTC TGTGAAAAAC AAAAAACAAA GGTTGGAAGT
    GATCCTGTTC TGTCTCCAAC AACACCTTCC TCAGGTGATC ACAAGAGTAT GCCTTTTCGC
    AAAGACACTC AGGCTAGAGA TTTCTTTAAG CATGGAAAAC ATCTCAAGGA GAAAGGCCTG
    GCAAGCCAAG TTGAGGTAGT GGATAAAGAG GAAAAAGTGA AAAACAAAAA ATATTTCAAA
    TCTGATCTTG CATTTGAATC CAGCTCTTCT GTGGATGCTG ACCGCAAGGC TGCACGAAAG
    AGACGCTTTG AAGAAACATC GGCTAAGGCT GATAATTTAA GGAGAGTAAG TCAGGAGGAG
    GATGAAGGGA AATTAAGGAG AATTATTGAT GAACCTTTGC TGAAAGACAC TGATTATGAT
    AAAAAGTTGC TTCGGAAAGA AGCACACAAG AGAGAGCGAA AAATTAAACC AGAGAGGATG
    GTTACTGTTA GTACTACAAT AGAAGAGTTA GACAATGTAA TGCCAGTAGG CCCCAGTTTA
    GACCTTCAGG CTCGACTTGG GGAACCAGCT GAAGATGCAA TAGATCCATT AGATAGCCTT
    GATCAAAAGA TAGGTGCTTT TGGAGCTAAC AGACAACCAA GTTTCAGTCT TGCAGTCTCC
    GACGATGGAA GTGTAGATAT GGATTTTTCA AGAGAGCAAG AGCAGCAGCA TTTAACTAGC
    TACCACACAT TATCCTCAAG GCAAGAAAGA GTTTCTGAAA GTAAGGAAAG TTTGCTCTCA
    GACATTGATC ATTCGCAGAG TTGCAGAAAA CAAATGGAGC AAAATCGCCG CTTACAGCAG
    CAAATGCTGG AGTGTGATAA ATCAGATAAA ACTGAAAGCA CACCAAGCAC TGATGCGGAG
    GAATTTGAGC ATCGAAGTAT TGTGCATGAG GTTGTAAAAC CATTACAAGA TGTAACGGGA
    AGTTCTCCGA CCAGCAAGCA AAAAAAATTG GGAGGATTCG AATTTGATTT TGGCAAAAGA
    GAGCAAAATT ATGAAATGTT TCGACTGAGA AATGATGAAC CTGAAAGAGG ACTTGCTTCA
    CATCCAGGGA CACCCCTTGC TGAGGAGGAG AGAAATGCAT CACAATTGTT GGACAAAGAC
    CCAGATTTAC CAGTGACAAT GGATAAAAAT AGCTCGCATT TAGATGTTTC AAAATACAAT
    ACTAGCAATG CAGTGCATCA AGGGCTTTCA CCACATGCAG AGATACTAAA AGTGAAACCA
    TCAGTTACTA AAGAGGAGTT CAGTTGGGAG AGCAATATTA GGCAGGGCAC ATTGAGAGAA
    ATGAGCTTTC CCACAAGCAT TGTTAAACGG GAAAGTATCA GAAAACGTCC TGAACGCGAA
    TTAGAACCTG GAGAGGTTCA GTCAGACTCT GATGAAGATG AAAGCAGACA CTATTCATTA
    AAGCCAATTT CTTTCAAAAG AGAGCATGAA GAGCGGCTTT CAGATGTAAA GTATTCTGAG
    TCTCTTGAAA AGAATAAGTT CTATGAATTT GCATTGGATA AGACGATAAC ACCAGACACA
    AAAGCTTTGC TTGAGCGTGC TAAGTCACTG TCTTCATCTA GAGAAGAAAA TTGGTTTTTC
    CTTAATCATG ACTCAAAATT CAAAAGTTTA CAGAATAACA TCGACAAGGA AAAGTCTGAA
    CCTACTCCAA GACCTATTCC TTCATGGTAC TTGAAGAAAA AGAAAAGTCG CTCTGACTCT
    GATGGAAAAC TTGATGAAAA AAAGCAAGAT GCTAAGCCTG ATGAACAAGA AAGACAAGAT
    CTCCTAGCTT CACGATTTCT TCATAGCTCA ATCTTTGAAC AGGATTCACG TCGGCTTCAA
    CACCTTGAAC GGAAAAATAC TGACCCTGAC GTTAGAATTG GCAGAGATAG TGTTACAAGT
    AATTCTCAAG GTGAACAACC TGGAACAGGA GGATCAGACT TGACTCAAGA ACCAAGAGTA
    CTTTTTCATA GCCGCTTTTT GGAACTTCAG CAAAGAGACA AGAACCAACA GCTTCCTATT
    TCAGAAAAAC TTAGTTTAAC TGATCAAATG GAGAAGACAT TGGATGAAGA GTTTAGGCCA
    TCTCCCAATT TATCAGACAT TTCCCAAGAG TCATTAGCAA GTCATAGCTT AACACCTACA
    ATATCCCCAG TGTCTCAGTC TAAAATTTCA GAGATGTCTG TGCACCATGA GAAGCCTGTT
    TTGCATACTA CTTTACCAAA CATACATAAT CCAGCAGTTG TGAATGTTAG GTCAGAAAAT
    GCTCCAATAG ATTATCCATC GTTAATTAGC CTAAAGGATG AGAACAACAC TATTCCGTTG
    AGCACTCCAT CACCTGAGCC CATGATAAAG GAAGATGTAA TTGTTGAAGA ACCTGAAGAA
    AAACTCAGAG ATTCCAGAAA AACTGTACAC GCCGCTGTTG AACACGAGAT TGAGATCAAA
    CCTCCCACTC CTGGTGCATC TTTAGGTAAT GTTGAGCCAG AATGTGATGC TCTGCAGGCA
    TCTTCTCCAT TGTATCCAAA TCCCAGTAAA GATCAAGAGA CTACAGAGCT CTCAGAAACA
    ACAACAGAGC CTGCTAATTC TTCTCTTGTT GTATCTCCTC TGGAGGTGCC AGACACAGAA
    ATTGAGCAAG TTGAACAAGT ACTACCACCG CGTAAACAAC CAAAGAGTAA AAAGGTAAAG
    AATGTCTCAT CAGCGCAAAC ACCAATCACC CAAGTCGTTA ATGAAAAACC AGCTACACGA
    AAGAGTGAAC GCATTGATAA AGAAAAGCTA AAACGATCCC CCAGAGGAGA CTCTACAAAA
    CTTACTGCTG ATTCCAGAAA TTCTGCAAAA TCTCCTGTTC AAGTTTTAGA TTCAGAGCAG
    GGCCTTGAAT TAAACACAAA CCAAGGCAGA ACACGCCAAC GACGGAATGT GCGATCTGTC
    TATGCAACAC CCCCAGAGGA AGAATCTCCT CAACAACAAG GAAAAGAGGT AACAGAACCT
    CCCCGTTCCA CTCTTAAGCG CTCTAGGGGT CGTCCACCCA AGACTCGCAG ACGAGTGGAT
    GATATGTCTC CGGTAAAAGG AGATCAGATC AAAAATTCTG AGGCTGAAGA CACTGAAAAT
    AAAGAGTCAA CAAGTAGTGG AGAAATAACT AAAAGTACAG AGGCATGGCG CTCTCCACGT
    TCTCAAAAGG GCCAGTCTTC ACCGATAAGA TCAGGACAAA GCAAAAAGAC ATTGAAATTG
    GACAAAGTGT CAGGTACTTC GGCATGTGTT CAATTAGACA GAGAAGCAGT TGATGTGCCA
    CCTGCCCTAG ATCGCAAAGC TGAATCCAAA GAAACTTTGA TGCAAGTTGG ACATTTGTCA
    AAAGATACCA AACAGAACAG CATATCTACA AGAGAAAAAG AACTTGTTGC TGAGGAAATT
    TCAACAGATG ACCGTGTAGA AATCACTCCT GTAGAAAAAA TATTAGACAA GGAGGTCAAG
    ACATCAAGGT CAACACGGAA CACAAAAACA ATGTCTGATA ATAAGGCTGT CAATGTGGTG
    AAGCTCACTA TGGATGCAGT TAAGGAAGCT GTTCATTCAG GTGATGACAT AACTGTTTGT
    TTTGAAGGGT CTTTAAAAGC TAATGTTCCA CAATCAGTAA TAGAACAGTC AGACATTCAA
    GATTATCACA AAAAGGAAAA TGTTAACAGC CTTGATGAAA AGGATGAGAC TCTTTCAGAA
    ATGGAGCCTC CTACTGATCC TGTGGCTGCC CTGCTTGCTC GCCAAATGGA ACTGGAAAGA
    GCAGTGGAAA ATATTTCAAG ATTTACAGAT GACCAGCATC CAGTACCTTA CAAAGAACCT
    CGTACAGAAC CACCTACCCT AACAGCACCT GTTGTAGTAC AACCTGCAGA GGATACAGAG
    GTAGGAAAGC CTGCCAATCC AGCAAGTGAA ACTGAATTGG CTGAAGCAAT TAATTCAATT
    ACTGCTGAGG ATATATCAGG TGATACAGAT GGATTCTCTG CTTCAACAAC ATACACAACC
    CTCCTTCCTC AACCAGAGAC TCTTGATTTG CCTGTACCAA ATGAGGTTGT GGAAACTGAG
    TCAGACTTGA CTGTAAAGAC TATTGCACAG TCTGAACAAG ATAATGTTAT GGTTCCAGTT
    TCAAAATCTT CTAAATGTAA AACAGATACT TCACTCAAAG AATCACCATT GACAGAGGTA
    GCAAAAAGAG GAGGAAGAGC ACGTCCAAAA ACTGCAAAAA AATCCAGAGG GAGGAAGGTT
    TCTTTGAACA GAAAATTAGA TATTGCTGAG GATGTAGTGC TGGAACCTGA ATCCACTACA
    GTCAAACTTC CAGAATCGAT TCCAGAAGAG ATTCAAACAG TTAACCCTAA GGCAGCAACA
    TCGGCAGCTG CAGCAGCTGT AGTCACAGTT GCTGCTGCAT GCAAACATGA AGCTGCATCT
    ACAGTGATTT TGAATACACC AAAAGAGGCA GAACAGCCTG CAGTTGATCA GCCTGAACCT
    CAAGAATCTG CCTTCCATTC TGGAAATAAC AGTCCTTCTT ATTTGAGGAC ACAACAGTCA
    TCTCCTGAGC GAGGAGCATC TGCCCTTACT TCACCTACAA CACGCCTAAA TTCACCTGCA
    TCCTCTAACA TTCCTCCTGA ATGGAATACC AGGACAGAGG AGAAAGTAAT GCTACCTAAA
    CCACAAGGTA ATGTGGCTTT GTCTATCCCA GGAGCTGGGG GACCCCCAGC AAATCCTCCC
    ATGCCTCCTG ACACAAAAGC CTCTGATATT AATGCAAGTT CTAGTACACT GCGAAAAATT
    TTAATGGAAC CAAAGTATGT TTCTGCTTCA AATAGCAATT CAGTGACAAG TATGCAGTTT
    ACTACCACAT TAGCAGATCC ACGGATGTCT GATAATGAAA ATTCAGTTGA GGCTGTGCTG
    CCTTTAAAAA CCTCTCTACC TGAAGATAGA CCTAGCCCTA TAGCTCAGCT TGTACCCCGT
    ACAACACCAC CGCAGCCCCC TCCTCTACAA CAATGTGAGA CTCCACAGAT CTTAAAGGAG
    AAACTAGCTA TAACTTCCAC TGCCACTTCA GTCATTAGTC GGATCCCTAT GCCCTTTGAT
    TTTGAGGACA CTCCTCGCAT CTCTTTAAGT AATCGTAACT CTGGAATGTC TTTGCCAAAG
    CAGAAATATC GTACTGGCTT GAATGAAAAT AATAGATATC ATGGACATAA TACTTCTGAG
    GATGGTGGAA GTGTTGGGCG CCCAGTTGTT GAAGGCACAC ATTGTAACAC GGGGTCTAGT
    ACTGGTTTGA GGGTCAACAC ATCTGAGGGT GTGGTAGTAT TAAGTTATTC TGGACAAAAG
    ACAGAGGGAC CACAGCGGAT CATTGCAAAA ATTAGCCAAA TCCCTCCAGC CAGTGCAGTT
    GACATTGAAT TTCAGCAGTC TATAACAAAA TCTCAGATTA AACAAGAACC TTCATCTCAC
    CCCTCTACCC CAAAAGGATC ACAGACACCT ACAGGCTATA GTCACACAGG GGTAGTATTA
    GCTGGCCAAA GTATCAATGC CCAACCTGTT ATATCCTCAA TACGCCAGGA AAGCCCTGGC
    TCTGAAAAAT CAGAAACATC TTACCAACAA GGAAGTTCTC TGAAATCTTT CCACCAGTCC
    CCAAGCCATC CTCAACTTCT GAGGTATGGT CAGTCAATAA CACCGCAACA CCATATGAAG
    AAAAATGGAG AAACTGAATC ATTTTCAATG AAAGCTGATA TAAAACCTGA TATAAAGGCA
    ACACAGACTT TCAATGTTAA ACCAGTTTTA AGTCCACGCC ACCCATCTTT CTCAGGCAAT
    CACATTTTAA GTCCAAGTGC TCCACATGAA AGAGCTGTCC CACAACCCAA GCAAGATTCA
    CATTCTCCAA GGGCATCTAA CCATTCCATG TCACCCTTTC CAAAGGTTTG CCCCCCTAAC
    AGCCCCGTTG TTCTGGGACC AGCTGGCCCT ATGTCTCAGT ATGTGTCAAA TATACACCAT
    GCTGAGCAGT CAGTGATCAT GCCTCCTCAT AGTGTCACCC AGTCTGTGCC CATGAGTCAT
    TTGTCCCAAG GGGATGTCCG AGCCAGTACT CCAACATTAT CTGGAATAGG TTATGGATTG
    CGTTCAGAAA ATCTCTTATC ACCTCGATCG GCACCTCCTC AACGTTCCAC GACTCCTCAA
    CCTGCAGTGA TCAGAGATGT CATATTGAAA TCGCATGCAG GCTCGTCTGT TGGTGGCCAT
    GTTGTTGAGA CGAGCAATGA TGATGCACAG AATCTCCCAC AAGGACTTCG TAGATCTTCT
    GCCCCACAGT TGCAACAGGA AAGTATGGTA ATACAGTCGG AATTCAAAAA TCTGCATCAC
    AGGGCATTAC GTCTAGATCA GTATGCCAGA CTTATGCAGC AGCATTTGAC TGACCATCCA
    GGTGTTGTTG AAAGTCGCCA AACTCGAACT GCAGAGGTGG TGCAGTCATC TTCACACATA
    TCATCTGCTT CTTCTAAAGC CTCTTCCATA GTAAAGAATG CTCCACAGAT TGTAAGGGAT
    GGACAAAAAA CAGTGGAGCT GAAGATGTCA CCCTCCCCTC ATTCAGACAA CAGGATAACA
    GGACACCCAC CAGGCTCAGT GATGGTATCT CCTCAAGGAG TTCAGTTAAA TTACCCTGGA
    AATGGAAATA CCATGAATGA ATACTACAAA GAAATGCGTG GCTTCCATCC TCAGTATCCA
    GGTCATTCAG TAATTGGAAT TAACTTGGCT AACCGTGGGA TTCCTGTGTC TCAGGTCTCA
    CAAATTGATC ACAGTCAAAG ACACAAGGTT CCCTCTCTCT CTTCCAGTGA GTCTGTGGGA
    AGTTTTAGTG AGTCAAAACT TGAGGGTTCT CACATTCGAC ACTCAGGCAC CATGGATTTG
    TCTCATATAT CACGAGTTCA GAGTGAGGCT GGGTCCCCCT CTTATATTTC TCCAGTGACT
    ATAACACCCA AACTGGAGTT GGCTATTACT TTGCAGAAGG GGCCTCAAGG ACCTGTTTCC
    AACAAGATGC CACTTCCTTC TTTAGCTTCT TCACAGATGC GTTCAGACTT CAAACTTGAC
    CACACAGGAC TTCGGTCTGT TGATATGGTG CAGTTGTTAA CGAAGTACCC AATCATTTGG
    CAAGGTCACC TGGCACTTAA GAATGACACT GCAGCAGTGC AGCTGCACTT TGTGTCTGGT
    AATAATGTTC TGGCTCATCG TTCACTGCCA CCCCCAGAAG GAGGTGCCTT TCTTCGCATT
    GCTCAGAGAA TGCGCTTAGA GGCTTCACAA CTGGAAGGAG TTGCGAGACG CATGACTGCT
    GAAAATGAAT ACTGCCTTCT GTTGGCATTG CCTTGTGGAT TGGACCAAGA AGATGTCCAC
    AACCAGACCC ATGCTCTTAA AACTGGTTTT ATTACTTACC TGCAAGCAAA ACAGGCTGCT
    GGAATTATCA ATGTCCCAAA CCCAGGCTCA AATCAGCCTG CCTACGTTGT CCAGATTTTC
    CCTCCTTGTG AGTTTTCTGA GAGCCACCTG TCTCATCTTG CTCCGGACCT TCTCAACAGC
    ATCTCCAGTA TCTCTCCTCA TCTTATGATT GTTATAGCTT CTGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.