ORF » Species Summary » Danio rerio » nit2 cDNA ORF clone

nit2 cDNA ORF clone, Danio rerio(zebrafish)

The following nit2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nit2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa60486 NM_205611.3
Latest version!		 Danio rerio nitrilase family, member 2 (nit2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    nit2 ( NM_205611.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991174.3 Danio rerio nitrilase family, member 2 (nit2), mRNA.
    Price & Availability↑
    CloneID ODa60486
    Clone ID Related Accession (Same CDS sequence) NM_205611.3
    Accession Version NM_205611.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product omega-amidase NIT2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH582542.1, BX324166.8 and EB850510.1. On Nov 2, 2015 this sequence version replaced NM_205611.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC094964.1, GDQH01003844.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCTGGTA TCGTTAAGGC AATGTCGAAG TTTCGACTGG CTGTGGTGCA GTTGCATGTG 
    TCAAAGATTA AAGCAGACAA TTTAGGAAGA GCACAAACAC TTGTGACGGA GGCTGCAGGA 
    CAAGGTGCAA AAGTTGTTGT TTTACCAGAG TGCTTCAACT CGCCTTATGG AACTGGGTTT 
    TTTAAAGAGT ATGCTGAGAA AATTCCTGGA GAGTCCACGC AGGTGTTGTC AGAGACCGCC 
    AAAAAGTGTG GGATCTATTT AGTTGGTGGT TCCATTCCAG AGGAGGATGG AGGGAAGCTC 
    TACAACACCT GCTCGGTTTT TGGACCTGAT GGAAAGCTGC TTGTCACACA CAGAAAAATT 
    CATCTCTTTG ATATCGACGT ACCAGGAAAA ATACGATTTC AGGAGTCTGA AACACTAAGT 
    CCAGGCAAAA GTTTATCTAT GTTTGAGACC CCATATTGTA AAGTAGGTGT GGGAATTTGT 
    TATGACATCC GGTTTGCAGA GCTTGCACAA ATCTATGCTA AGAAAGGTTG CCAGCTTTTG 
    GTGTATCCTG GTGCTTTCAA TATGACCACA GGTCCTGCTC ACTGGGAGCT GCTGCAGAGG 
    GGGAGGGCAG TAGATAATCA AGTCTATGTG GCCACTGCTT CACCTGCAAG AGATGAGACA 
    GCCAGCTATG TGGCTTGGGG TCACAGTTCT GTGATAAACC CTTGGGGGGA AGTAATTAGC 
    AAAGCCGGAT CAGAGGAATC TGTTGTTTAT GCTGACATAG ACCTGCAGTA TTTGGCTGAT 
    GTGCGTCAAC AGATCCCCAT CACCAAACAG CGACGGAATG ATCTATATAG TGTTAACAGT 
    GTTCAGGAGG GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_991174.3
    CDS78..932
    Translation

    Target ORF information:

    RefSeq Version NM_205611.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio nitrilase family, member 2 (nit2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_205611.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCTGGTA TCGTTAAGGC AATGTCGAAG TTTCGACTGG CTGTGGTGCA GTTGCATGTG 
    TCAAAGATTA AAGCAGACAA TTTAGGAAGA GCACAAACAC TTGTGACGGA GGCTGCAGGA 
    CAAGGTGCAA AAGTTGTTGT TTTACCAGAG TGCTTCAACT CGCCTTATGG AACTGGGTTT 
    TTTAAAGAGT ATGCTGAGAA AATTCCTGGA GAGTCCACGC AGGTGTTGTC AGAGACCGCC 
    AAAAAGTGTG GGATCTATTT AGTTGGTGGT TCCATTCCAG AGGAGGATGG AGGGAAGCTC 
    TACAACACCT GCTCGGTTTT TGGACCTGAT GGAAAGCTGC TTGTCACACA CAGAAAAATT 
    CATCTCTTTG ATATCGACGT ACCAGGAAAA ATACGATTTC AGGAGTCTGA AACACTAAGT 
    CCAGGCAAAA GTTTATCTAT GTTTGAGACC CCATATTGTA AAGTAGGTGT GGGAATTTGT 
    TATGACATCC GGTTTGCAGA GCTTGCACAA ATCTATGCTA AGAAAGGTTG CCAGCTTTTG 
    GTGTATCCTG GTGCTTTCAA TATGACCACA GGTCCTGCTC ACTGGGAGCT GCTGCAGAGG 
    GGGAGGGCAG TAGATAATCA AGTCTATGTG GCCACTGCTT CACCTGCAAG AGATGAGACA 
    GCCAGCTATG TGGCTTGGGG TCACAGTTCT GTGATAAACC CTTGGGGGGA AGTAATTAGC 
    AAAGCCGGAT CAGAGGAATC TGTTGTTTAT GCTGACATAG ACCTGCAGTA TTTGGCTGAT 
    GTGCGTCAAC AGATCCCCAT CACCAAACAG CGACGGAATG ATCTATATAG TGTTAACAGT 
    GTTCAGGAGG GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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