ANKRD27 cDNA ORF clone, Danio rerio(Zebrafish)

The following ANKRD27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa141495 XM_021470745.1
Latest version!
Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa141496 XM_021470746.1
Latest version!
Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa141497 XM_021470747.1
Latest version!
Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa141497 XM_021470748.1
Latest version!
Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa141498 XM_021470749.1
Latest version!
Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa57976 XM_017354281.1
Latest version!
Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa57977 XM_002666959.5
Latest version!
Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa57977 XM_017354282.1
Latest version!
Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa141495
    Clone ID Related Accession (Same CDS sequence) XM_021470745.1
    Accession Version XM_021470745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ankyrin repeat domain-containing protein 27 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 983 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
    TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG
    TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC
    CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA
    CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG
    GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT
    GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG
    AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT
    GCTGAGGAGT TGGGTTCTGT TCCTGCTCCG TACTGTCTGA AAAATATTGA GGATGTGAGG
    GAGTTTTTGG GCCGCCATGC TGAAAAACTG GACAAGTTTG TTTCCTCCTT CTGTCTCTCC
    TTTAAAGAAC AGGAGAGGAA AGGCCTACGC CATCACATAG ACTCGGTGAA TACGCTTTAC
    ACCAGATGTC TTCAGTGTCT GCTGAGAGAC TCTCGTCTGA AGCTTTTGGC GAGACAGGAG
    ATTCAGATGA CTCTACTCAA AGAAGCAGTA GAGATGTACA TCCATCATGG CATCCATGAT
    CTACTCTTTA GTTATGTGGG CACTCTTGAA GCTAGTCGGG ATGCAGCGTT CAACAAAACA
    ACACGGTCCT TGCAGGATCT GCAGCAGAAA GAGCTGGGAG TGAAGTCCGA GTTCAGTATT
    AATATTCCTC GGGCGAAGCG TGAGCTGATT CAGCTGAACG TCTGCACGTC TCCACAGCAG
    AAACTCCTCT GCATCCGGAA AGTCATTTTA ACCATCATGC AGTCGACCAG ACGCAGAGAT
    ACAGCGGTGA GCATCGAGTC CATGTGTGCC GATGATCTGC TGTTGGTCAT TCTGTATCTG
    CTCATCAAGA CAGAAATTCC CAACTGGATG GCCAATCTGA GTTATATAAA GCACTTCCGC
    TTTCGGAGCT CCAGCAAGGA TGAGTTGAGT TACTGTCTGA CGTCTTTCGA GGCCGCGGTC
    GAGTATATCA GCCTGGGAAA CCTCAAACAC AACACCGATG GGTCAGGAAA CAGACTGTCC
    TTCAGGCAGA AGGTGGACCT ACTGTCCCAG AATGCAACAC CCATTGACCG GCTGTTTGAG
    CATATTGTGA GCGGAAATGA GCAGGAGGTT CAGCGTTTGC TCAGTGAAAG CGAGAGTGAG
    GAGGACGGCA GGAAGTTGTG TCACCCTCTG TGTTCCTGTG ACGCCTGCGA CCTGCGCATC
    TCTGGGAGAT TGAATGATCC TTCCATCGTC ACTCCATTCT CTCGAGACGA CAGAGGATAC
    ACTCCTTTAC ATGTTGCTGC TATTTGTGGT CAGTCTCAGA TGATTGATTT ACTGGTGTCA
    AAGGGAGCAG TGGTAAACGC TACCGATTAC CATGCACTCA CACCGCTGCA CCTCTCCTGC
    CAGAAGGGCT ACCAGGGCGT CACGCTTCTC CTGCTGCATT ATAAGGCCAA CACTGATGCT
    CAGGACAACA ATGGAAACAC TCCTCTACAT CTGGCCTGCA TGTACGGACA TGAAGACTGT
    GTGAAGGCGC TGGTGTACTT CGATCTGCAC TGCTGTCGGC TGAACGTTCA GAACGATAAA
    GGAGACTCGC CGCTGCACAT CGCCGCTCGC TGGGGATACG AGGGCATCAT GGAGGTGCTG
    CTGGAGAACG GAGCGTCCAC GCTCATCCAC AATAAAGCCA AGGAGACGCC GCTGCACTGC
    GCGCTCAACT CCAAGATTCT GTCATTGTTG GAGCGCAGAC ACAACGACTC CAGTATAAGG
    GAAAGTGGAT TCGAGTCTCC GAGCCGCTCT CCTCCTCCGT CAGACGGCAG CAGCAGACGC
    TCCTCCATCT CCAGCTCATC CTCTCTGAGT ATTGAAACCT CTCCGCTGGA GTCTGACCGC
    AGCCGGCACA GAGAGGTGGA GAAGCTGTTG AGAGCCGTGG CTGACGGAGA TGTGGAGATG
    GTGCGTTATC TCCTGGAGTG GCTCGATGAG GATCCAGATG AAGAGGAGTC TGCAGTGCCA
    GTCAAGACGG AGCTCTGCCA TCCGCTGTGC CAGTGTGCCA ACTGCGAACC CACACAGAAG
    AAGGCAGCTC ATCTTCATCC AGACAGTGTG GGTGTAAACA GCAGCAGTGT TGACGGCTTC
    ACGCCGCTGC ATGTGGCGGC GCTGCATGGA CACACACCTC TCATCTCTCT GTTCACACGC
    CACGGCGCAA ACCTGAACGC TCGCAACAAC CACAGCGCCA CCCCACTGCA CCTGGCCTGC
    CAGAACAACC ACACAGAGGT GGTCTGTGCT CTCCTGGAGT GTAATGCCAA ACTCAATAAG
    AAGGATCATT ATGGGAACAC ACCGCTCATT CAGGCCTGTC TGAAGGGGAA TCTGGAGATC
    GCCGGCGTCC TGCTGGAGAG CGGTGCGCTG GTAAATCTGG CCAATAACCA CGGGAACACG
    GGTCTGCATG AGGCTGTGAG AGGAGCAAAC GTGTCGCTGG TGGAGCTGCT GCTGCACAGA
    GGAGCTTCAG TTCACATCCG CAACAACAGA CAGAGAACAT CGCTGGACTG CGCACACGAG
    GCCGCCGCCA AGAACTCAGA GATTTTGAGA CTCCTTCAGA AAGCATATGA TGAAGCCCAG
    ACGCAAAGGG CCAGAGCACC AGAGGGCATC ATGGCAAGAG TTAAACAGAG TCCTAACAAT
    GGTGGACAGG ATTTTGAGCA CCACAAACAG AGGAGTCTGA AGAGAACAGA CAGAGCGGCA
    GCTACAACAG CAGCAGCACA ACAGACTGCA GGGAGCGACA GACGGAGACT GACCCGAGGA
    GAAACACTGG ACATCAGTGA TCCACAGCCT CCGAGCAGAC CTCTGACCCG CAGCCACACC
    ATCAGCCAAG ACCTGAGTTC CAGCGTTCAG CCCGAAGAGC AGATCAACGG TGGAAAACGG
    GAGGATGAAG AAGATCCGTC CGGTGCTGAA GCAGATCCTG CAGACACTGA GATGGACAGC
    GTGGTGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326420.1
    CDS50..3241
    Translation

    Target ORF information:

    RefSeq Version XM_021470745.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
    TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG
    TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC
    CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA
    CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG
    GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT
    GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG
    AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT
    GCTGAGGAGT TGGGTTCTGT TCCTGCTCCG TACTGTCTGA AAAATATTGA GGATGTGAGG
    GAGTTTTTGG GCCGCCATGC TGAAAAACTG GACAAGTTTG TTTCCTCCTT CTGTCTCTCC
    TTTAAAGAAC AGGAGAGGAA AGGCCTACGC CATCACATAG ACTCGGTGAA TACGCTTTAC
    ACCAGATGTC TTCAGTGTCT GCTGAGAGAC TCTCGTCTGA AGCTTTTGGC GAGACAGGAG
    ATTCAGATGA CTCTACTCAA AGAAGCAGTA GAGATGTACA TCCATCATGG CATCCATGAT
    CTACTCTTTA GTTATGTGGG CACTCTTGAA GCTAGTCGGG ATGCAGCGTT CAACAAAACA
    ACACGGTCCT TGCAGGATCT GCAGCAGAAA GAGCTGGGAG TGAAGTCCGA GTTCAGTATT
    AATATTCCTC GGGCGAAGCG TGAGCTGATT CAGCTGAACG TCTGCACGTC TCCACAGCAG
    AAACTCCTCT GCATCCGGAA AGTCATTTTA ACCATCATGC AGTCGACCAG ACGCAGAGAT
    ACAGCGGTGA GCATCGAGTC CATGTGTGCC GATGATCTGC TGTTGGTCAT TCTGTATCTG
    CTCATCAAGA CAGAAATTCC CAACTGGATG GCCAATCTGA GTTATATAAA GCACTTCCGC
    TTTCGGAGCT CCAGCAAGGA TGAGTTGAGT TACTGTCTGA CGTCTTTCGA GGCCGCGGTC
    GAGTATATCA GCCTGGGAAA CCTCAAACAC AACACCGATG GGTCAGGAAA CAGACTGTCC
    TTCAGGCAGA AGGTGGACCT ACTGTCCCAG AATGCAACAC CCATTGACCG GCTGTTTGAG
    CATATTGTGA GCGGAAATGA GCAGGAGGTT CAGCGTTTGC TCAGTGAAAG CGAGAGTGAG
    GAGGACGGCA GGAAGTTGTG TCACCCTCTG TGTTCCTGTG ACGCCTGCGA CCTGCGCATC
    TCTGGGAGAT TGAATGATCC TTCCATCGTC ACTCCATTCT CTCGAGACGA CAGAGGATAC
    ACTCCTTTAC ATGTTGCTGC TATTTGTGGT CAGTCTCAGA TGATTGATTT ACTGGTGTCA
    AAGGGAGCAG TGGTAAACGC TACCGATTAC CATGCACTCA CACCGCTGCA CCTCTCCTGC
    CAGAAGGGCT ACCAGGGCGT CACGCTTCTC CTGCTGCATT ATAAGGCCAA CACTGATGCT
    CAGGACAACA ATGGAAACAC TCCTCTACAT CTGGCCTGCA TGTACGGACA TGAAGACTGT
    GTGAAGGCGC TGGTGTACTT CGATCTGCAC TGCTGTCGGC TGAACGTTCA GAACGATAAA
    GGAGACTCGC CGCTGCACAT CGCCGCTCGC TGGGGATACG AGGGCATCAT GGAGGTGCTG
    CTGGAGAACG GAGCGTCCAC GCTCATCCAC AATAAAGCCA AGGAGACGCC GCTGCACTGC
    GCGCTCAACT CCAAGATTCT GTCATTGTTG GAGCGCAGAC ACAACGACTC CAGTATAAGG
    GAAAGTGGAT TCGAGTCTCC GAGCCGCTCT CCTCCTCCGT CAGACGGCAG CAGCAGACGC
    TCCTCCATCT CCAGCTCATC CTCTCTGAGT ATTGAAACCT CTCCGCTGGA GTCTGACCGC
    AGCCGGCACA GAGAGGTGGA GAAGCTGTTG AGAGCCGTGG CTGACGGAGA TGTGGAGATG
    GTGCGTTATC TCCTGGAGTG GCTCGATGAG GATCCAGATG AAGAGGAGTC TGCAGTGCCA
    GTCAAGACGG AGCTCTGCCA TCCGCTGTGC CAGTGTGCCA ACTGCGAACC CACACAGAAG
    AAGGCAGCTC ATCTTCATCC AGACAGTGTG GGTGTAAACA GCAGCAGTGT TGACGGCTTC
    ACGCCGCTGC ATGTGGCGGC GCTGCATGGA CACACACCTC TCATCTCTCT GTTCACACGC
    CACGGCGCAA ACCTGAACGC TCGCAACAAC CACAGCGCCA CCCCACTGCA CCTGGCCTGC
    CAGAACAACC ACACAGAGGT GGTCTGTGCT CTCCTGGAGT GTAATGCCAA ACTCAATAAG
    AAGGATCATT ATGGGAACAC ACCGCTCATT CAGGCCTGTC TGAAGGGGAA TCTGGAGATC
    GCCGGCGTCC TGCTGGAGAG CGGTGCGCTG GTAAATCTGG CCAATAACCA CGGGAACACG
    GGTCTGCATG AGGCTGTGAG AGGAGCAAAC GTGTCGCTGG TGGAGCTGCT GCTGCACAGA
    GGAGCTTCAG TTCACATCCG CAACAACAGA CAGAGAACAT CGCTGGACTG CGCACACGAG
    GCCGCCGCCA AGAACTCAGA GATTTTGAGA CTCCTTCAGA AAGCATATGA TGAAGCCCAG
    ACGCAAAGGG CCAGAGCACC AGAGGGCATC ATGGCAAGAG TTAAACAGAG TCCTAACAAT
    GGTGGACAGG ATTTTGAGCA CCACAAACAG AGGAGTCTGA AGAGAACAGA CAGAGCGGCA
    GCTACAACAG CAGCAGCACA ACAGACTGCA GGGAGCGACA GACGGAGACT GACCCGAGGA
    GAAACACTGG ACATCAGTGA TCCACAGCCT CCGAGCAGAC CTCTGACCCG CAGCCACACC
    ATCAGCCAAG ACCTGAGTTC CAGCGTTCAG CCCGAAGAGC AGATCAACGG TGGAAAACGG
    GAGGATGAAG AAGATCCGTC CGGTGCTGAA GCAGATCCTG CAGACACTGA GATGGACAGC
    GTGGTGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141496
    Clone ID Related Accession (Same CDS sequence) XM_021470746.1
    Accession Version XM_021470746.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3189bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ankyrin repeat domain-containing protein 27 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 983 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
    TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG
    TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC
    CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA
    CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG
    GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT
    GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG
    AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT
    GAGGAGTTGG GTTCTGTTCC TGCTCCGTAC TGTCTGAAAA ATATTGAGGA TGTGAGGGAG
    TTTTTGGGCC GCCATGCTGA AAAACTGGAC AAGTTTGTTT CCTCCTTCTG TCTCTCCTTT
    AAAGAACAGG AGAGGAAAGG CCTACGCCAT CACATAGACT CGGTGAATAC GCTTTACACC
    AGATGTCTTC AGTGTCTGCT GAGAGACTCT CGTCTGAAGC TTTTGGCGAG ACAGGAGATT
    CAGATGACTC TACTCAAAGA AGCAGTAGAG ATGTACATCC ATCATGGCAT CCATGATCTA
    CTCTTTAGTT ATGTGGGCAC TCTTGAAGCT AGTCGGGATG CAGCGTTCAA CAAAACAACA
    CGGTCCTTGC AGGATCTGCA GCAGAAAGAG CTGGGAGTGA AGTCCGAGTT CAGTATTAAT
    ATTCCTCGGG CGAAGCGTGA GCTGATTCAG CTGAACGTCT GCACGTCTCC ACAGCAGAAA
    CTCCTCTGCA TCCGGAAAGT CATTTTAACC ATCATGCAGT CGACCAGACG CAGAGATACA
    GCGGTGAGCA TCGAGTCCAT GTGTGCCGAT GATCTGCTGT TGGTCATTCT GTATCTGCTC
    ATCAAGACAG AAATTCCCAA CTGGATGGCC AATCTGAGTT ATATAAAGCA CTTCCGCTTT
    CGGAGCTCCA GCAAGGATGA GTTGAGTTAC TGTCTGACGT CTTTCGAGGC CGCGGTCGAG
    TATATCAGCC TGGGAAACCT CAAACACAAC ACCGATGGGT CAGGAAACAG ACTGTCCTTC
    AGGCAGAAGG TGGACCTACT GTCCCAGAAT GCAACACCCA TTGACCGGCT GTTTGAGCAT
    ATTGTGAGCG GAAATGAGCA GGAGGTTCAG CGTTTGCTCA GTGAAAGCGA GAGTGAGGAG
    GACGGCAGGA AGTTGTGTCA CCCTCTGTGT TCCTGTGACG CCTGCGACCT GCGCATCTCT
    GGGAGATTGA ATGATCCTTC CATCGTCACT CCATTCTCTC GAGACGACAG AGGATACACT
    CCTTTACATG TTGCTGCTAT TTGTGGTCAG TCTCAGATGA TTGATTTACT GGTGTCAAAG
    GGAGCAGTGG TAAACGCTAC CGATTACCAT GCACTCACAC CGCTGCACCT CTCCTGCCAG
    AAGGGCTACC AGGGCGTCAC GCTTCTCCTG CTGCATTATA AGGCCAACAC TGATGCTCAG
    GACAACAATG GAAACACTCC TCTACATCTG GCCTGCATGT ACGGACATGA AGACTGTGTG
    AAGGCGCTGG TGTACTTCGA TCTGCACTGC TGTCGGCTGA ACGTTCAGAA CGATAAAGGA
    GACTCGCCGC TGCACATCGC CGCTCGCTGG GGATACGAGG GCATCATGGA GGTGCTGCTG
    GAGAACGGAG CGTCCACGCT CATCCACAAT AAAGCCAAGG AGACGCCGCT GCACTGCGCG
    CTCAACTCCA AGATTCTGTC ATTGTTGGAG CGCAGACACA ACGACTCCAG TATAAGGGAA
    AGTGGATTCG AGTCTCCGAG CCGCTCTCCT CCTCCGTCAG ACGGCAGCAG CAGACGCTCC
    TCCATCTCCA GCTCATCCTC TCTGAGTATT GAAACCTCTC CGCTGGAGTC TGACCGCAGC
    CGGCACAGAG AGGTGGAGAA GCTGTTGAGA GCCGTGGCTG ACGGAGATGT GGAGATGGTG
    CGTTATCTCC TGGAGTGGCT CGATGAGGAT CCAGATGAAG AGGAGTCTGC AGTGCCAGTC
    AAGACGGAGC TCTGCCATCC GCTGTGCCAG TGTGCCAACT GCGAACCCAC ACAGAAGAAG
    GCAGCTCATC TTCATCCAGA CAGTGTGGGT GTAAACAGCA GCAGTGTTGA CGGCTTCACG
    CCGCTGCATG TGGCGGCGCT GCATGGACAC ACACCTCTCA TCTCTCTGTT CACACGCCAC
    GGCGCAAACC TGAACGCTCG CAACAACCAC AGCGCCACCC CACTGCACCT GGCCTGCCAG
    AACAACCACA CAGAGGTGGT CTGTGCTCTC CTGGAGTGTA ATGCCAAACT CAATAAGAAG
    GATCATTATG GGAACACACC GCTCATTCAG GCCTGTCTGA AGGGGAATCT GGAGATCGCC
    GGCGTCCTGC TGGAGAGCGG TGCGCTGGTA AATCTGGCCA ATAACCACGG GAACACGGGT
    CTGCATGAGG CTGTGAGAGG AGCAAACGTG TCGCTGGTGG AGCTGCTGCT GCACAGAGGA
    GCTTCAGTTC ACATCCGCAA CAACAGACAG AGAACATCGC TGGACTGCGC ACACGAGGCC
    GCCGCCAAGA ACTCAGAGAT TTTGAGACTC CTTCAGAAAG CATATGATGA AGCCCAGACG
    CAAAGGGCCA GAGCACCAGA GGGCATCATG GCAAGAGTTA AACAGAGTCC TAACAATGGT
    GGACAGGATT TTGAGCACCA CAAACAGAGG AGTCTGAAGA GAACAGACAG AGCGGCAGCT
    ACAACAGCAG CAGCACAACA GACTGCAGGG AGCGACAGAC GGAGACTGAC CCGAGGAGAA
    ACACTGGACA TCAGTGATCC ACAGCCTCCG AGCAGACCTC TGACCCGCAG CCACACCATC
    AGCCAAGACC TGAGTTCCAG CGTTCAGCCC GAAGAGCAGA TCAACGGTGG AAAACGGGAG
    GATGAAGAAG ATCCGTCCGG TGCTGAAGCA GATCCTGCAG ACACTGAGAT GGACAGCGTG
    GTGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326421.1
    CDS50..3238
    Translation

    Target ORF information:

    RefSeq Version XM_021470746.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470746.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
    TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG
    TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC
    CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA
    CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG
    GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT
    GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG
    AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT
    GAGGAGTTGG GTTCTGTTCC TGCTCCGTAC TGTCTGAAAA ATATTGAGGA TGTGAGGGAG
    TTTTTGGGCC GCCATGCTGA AAAACTGGAC AAGTTTGTTT CCTCCTTCTG TCTCTCCTTT
    AAAGAACAGG AGAGGAAAGG CCTACGCCAT CACATAGACT CGGTGAATAC GCTTTACACC
    AGATGTCTTC AGTGTCTGCT GAGAGACTCT CGTCTGAAGC TTTTGGCGAG ACAGGAGATT
    CAGATGACTC TACTCAAAGA AGCAGTAGAG ATGTACATCC ATCATGGCAT CCATGATCTA
    CTCTTTAGTT ATGTGGGCAC TCTTGAAGCT AGTCGGGATG CAGCGTTCAA CAAAACAACA
    CGGTCCTTGC AGGATCTGCA GCAGAAAGAG CTGGGAGTGA AGTCCGAGTT CAGTATTAAT
    ATTCCTCGGG CGAAGCGTGA GCTGATTCAG CTGAACGTCT GCACGTCTCC ACAGCAGAAA
    CTCCTCTGCA TCCGGAAAGT CATTTTAACC ATCATGCAGT CGACCAGACG CAGAGATACA
    GCGGTGAGCA TCGAGTCCAT GTGTGCCGAT GATCTGCTGT TGGTCATTCT GTATCTGCTC
    ATCAAGACAG AAATTCCCAA CTGGATGGCC AATCTGAGTT ATATAAAGCA CTTCCGCTTT
    CGGAGCTCCA GCAAGGATGA GTTGAGTTAC TGTCTGACGT CTTTCGAGGC CGCGGTCGAG
    TATATCAGCC TGGGAAACCT CAAACACAAC ACCGATGGGT CAGGAAACAG ACTGTCCTTC
    AGGCAGAAGG TGGACCTACT GTCCCAGAAT GCAACACCCA TTGACCGGCT GTTTGAGCAT
    ATTGTGAGCG GAAATGAGCA GGAGGTTCAG CGTTTGCTCA GTGAAAGCGA GAGTGAGGAG
    GACGGCAGGA AGTTGTGTCA CCCTCTGTGT TCCTGTGACG CCTGCGACCT GCGCATCTCT
    GGGAGATTGA ATGATCCTTC CATCGTCACT CCATTCTCTC GAGACGACAG AGGATACACT
    CCTTTACATG TTGCTGCTAT TTGTGGTCAG TCTCAGATGA TTGATTTACT GGTGTCAAAG
    GGAGCAGTGG TAAACGCTAC CGATTACCAT GCACTCACAC CGCTGCACCT CTCCTGCCAG
    AAGGGCTACC AGGGCGTCAC GCTTCTCCTG CTGCATTATA AGGCCAACAC TGATGCTCAG
    GACAACAATG GAAACACTCC TCTACATCTG GCCTGCATGT ACGGACATGA AGACTGTGTG
    AAGGCGCTGG TGTACTTCGA TCTGCACTGC TGTCGGCTGA ACGTTCAGAA CGATAAAGGA
    GACTCGCCGC TGCACATCGC CGCTCGCTGG GGATACGAGG GCATCATGGA GGTGCTGCTG
    GAGAACGGAG CGTCCACGCT CATCCACAAT AAAGCCAAGG AGACGCCGCT GCACTGCGCG
    CTCAACTCCA AGATTCTGTC ATTGTTGGAG CGCAGACACA ACGACTCCAG TATAAGGGAA
    AGTGGATTCG AGTCTCCGAG CCGCTCTCCT CCTCCGTCAG ACGGCAGCAG CAGACGCTCC
    TCCATCTCCA GCTCATCCTC TCTGAGTATT GAAACCTCTC CGCTGGAGTC TGACCGCAGC
    CGGCACAGAG AGGTGGAGAA GCTGTTGAGA GCCGTGGCTG ACGGAGATGT GGAGATGGTG
    CGTTATCTCC TGGAGTGGCT CGATGAGGAT CCAGATGAAG AGGAGTCTGC AGTGCCAGTC
    AAGACGGAGC TCTGCCATCC GCTGTGCCAG TGTGCCAACT GCGAACCCAC ACAGAAGAAG
    GCAGCTCATC TTCATCCAGA CAGTGTGGGT GTAAACAGCA GCAGTGTTGA CGGCTTCACG
    CCGCTGCATG TGGCGGCGCT GCATGGACAC ACACCTCTCA TCTCTCTGTT CACACGCCAC
    GGCGCAAACC TGAACGCTCG CAACAACCAC AGCGCCACCC CACTGCACCT GGCCTGCCAG
    AACAACCACA CAGAGGTGGT CTGTGCTCTC CTGGAGTGTA ATGCCAAACT CAATAAGAAG
    GATCATTATG GGAACACACC GCTCATTCAG GCCTGTCTGA AGGGGAATCT GGAGATCGCC
    GGCGTCCTGC TGGAGAGCGG TGCGCTGGTA AATCTGGCCA ATAACCACGG GAACACGGGT
    CTGCATGAGG CTGTGAGAGG AGCAAACGTG TCGCTGGTGG AGCTGCTGCT GCACAGAGGA
    GCTTCAGTTC ACATCCGCAA CAACAGACAG AGAACATCGC TGGACTGCGC ACACGAGGCC
    GCCGCCAAGA ACTCAGAGAT TTTGAGACTC CTTCAGAAAG CATATGATGA AGCCCAGACG
    CAAAGGGCCA GAGCACCAGA GGGCATCATG GCAAGAGTTA AACAGAGTCC TAACAATGGT
    GGACAGGATT TTGAGCACCA CAAACAGAGG AGTCTGAAGA GAACAGACAG AGCGGCAGCT
    ACAACAGCAG CAGCACAACA GACTGCAGGG AGCGACAGAC GGAGACTGAC CCGAGGAGAA
    ACACTGGACA TCAGTGATCC ACAGCCTCCG AGCAGACCTC TGACCCGCAG CCACACCATC
    AGCCAAGACC TGAGTTCCAG CGTTCAGCCC GAAGAGCAGA TCAACGGTGG AAAACGGGAG
    GATGAAGAAG ATCCGTCCGG TGCTGAAGCA GATCCTGCAG ACACTGAGAT GGACAGCGTG
    GTGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141497
    Clone ID Related Accession (Same CDS sequence) XM_021470748.1 , XM_021470747.1
    Accession Version XM_021470748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ankyrin repeat domain-containing protein 27 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 983 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
    AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT
    GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT
    TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC
    CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC
    TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC
    CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG
    GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC
    TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT
    ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG
    AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC
    ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC
    AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG
    TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT
    CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA
    CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT
    CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG
    CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG
    GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC
    AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG
    CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC
    GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC
    CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC
    AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA
    CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC
    CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC
    ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT
    GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG
    AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG
    GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG
    CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC
    AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC
    AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG
    TCTGACCGCA GCCGGCACAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT
    GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTCGATGAGG ATCCAGATGA AGAGGAGTCT
    GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC
    ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTAAACAG CAGCAGTGTT
    GACGGCTTCA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG
    TTCACACGCC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC
    CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA
    CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT
    CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC
    GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGTCGCTGGT GGAGCTGCTG
    CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACATC GCTGGACTGC
    GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT
    GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT
    CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC
    AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG
    ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC
    AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT
    GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG
    ATGGACAGCG TGGTGTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326423.1
    CDS41..3121
    Translation

    Target ORF information:

    RefSeq Version XM_021470748.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
    AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT
    GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT
    TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC
    CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC
    TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC
    CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG
    GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC
    TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT
    ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG
    AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC
    ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC
    AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG
    TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT
    CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA
    CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT
    CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG
    CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG
    GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC
    AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG
    CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC
    GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC
    CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC
    AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA
    CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC
    CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC
    ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT
    GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG
    AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG
    GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG
    CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC
    AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC
    AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG
    TCTGACCGCA GCCGGCACAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT
    GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTCGATGAGG ATCCAGATGA AGAGGAGTCT
    GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC
    ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTAAACAG CAGCAGTGTT
    GACGGCTTCA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG
    TTCACACGCC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC
    CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA
    CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT
    CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC
    GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGTCGCTGGT GGAGCTGCTG
    CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACATC GCTGGACTGC
    GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT
    GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT
    CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC
    AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG
    ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC
    AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT
    GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG
    ATGGACAGCG TGGTGTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141497
    Clone ID Related Accession (Same CDS sequence) XM_021470748.1 , XM_021470747.1
    Accession Version XM_021470747.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ankyrin repeat domain-containing protein 27 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 983 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
    AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT
    GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT
    TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC
    CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC
    TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC
    CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG
    GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC
    TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT
    ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG
    AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC
    ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC
    AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG
    TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT
    CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA
    CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT
    CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG
    CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG
    GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC
    AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG
    CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC
    GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC
    CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC
    AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA
    CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC
    CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC
    ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT
    GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG
    AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG
    GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG
    CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC
    AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC
    AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG
    TCTGACCGCA GCCGGCACAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT
    GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTCGATGAGG ATCCAGATGA AGAGGAGTCT
    GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC
    ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTAAACAG CAGCAGTGTT
    GACGGCTTCA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG
    TTCACACGCC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC
    CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA
    CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT
    CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC
    GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGTCGCTGGT GGAGCTGCTG
    CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACATC GCTGGACTGC
    GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT
    GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT
    CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC
    AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG
    ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC
    AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT
    GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG
    ATGGACAGCG TGGTGTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326422.1
    CDS171..3251
    Translation

    Target ORF information:

    RefSeq Version XM_021470747.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
    AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT
    GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT
    TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC
    CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC
    TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC
    CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG
    GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC
    TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT
    ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG
    AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC
    ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC
    AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG
    TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT
    CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA
    CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT
    CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG
    CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG
    GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC
    AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG
    CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC
    GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC
    CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC
    AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA
    CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC
    CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC
    ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT
    GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG
    AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG
    GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG
    CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC
    AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC
    AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG
    TCTGACCGCA GCCGGCACAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT
    GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTCGATGAGG ATCCAGATGA AGAGGAGTCT
    GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC
    ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTAAACAG CAGCAGTGTT
    GACGGCTTCA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG
    TTCACACGCC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC
    CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA
    CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT
    CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC
    GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGTCGCTGGT GGAGCTGCTG
    CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACATC GCTGGACTGC
    GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT
    GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT
    CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC
    AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG
    ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC
    AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT
    GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG
    ATGGACAGCG TGGTGTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141498
    Clone ID Related Accession (Same CDS sequence) XM_021470749.1
    Accession Version XM_021470749.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product ankyrin repeat domain-containing protein 27 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 983 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
    AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT
    GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT
    TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC
    CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC
    TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC
    CACATTCCTG AGGAGTTGGG TTCTGTTCCT GCTCCGTACT GTCTGAAAAA TATTGAGGAT
    GTGAGGGAGT TTTTGGGCCG CCATGCTGAA AAACTGGACA AGTTTGTTTC CTCCTTCTGT
    CTCTCCTTTA AAGAACAGGA GAGGAAAGGC CTACGCCATC ACATAGACTC GGTGAATACG
    CTTTACACCA GATGTCTTCA GTGTCTGCTG AGAGACTCTC GTCTGAAGCT TTTGGCGAGA
    CAGGAGATTC AGATGACTCT ACTCAAAGAA GCAGTAGAGA TGTACATCCA TCATGGCATC
    CATGATCTAC TCTTTAGTTA TGTGGGCACT CTTGAAGCTA GTCGGGATGC AGCGTTCAAC
    AAAACAACAC GGTCCTTGCA GGATCTGCAG CAGAAAGAGC TGGGAGTGAA GTCCGAGTTC
    AGTATTAATA TTCCTCGGGC GAAGCGTGAG CTGATTCAGC TGAACGTCTG CACGTCTCCA
    CAGCAGAAAC TCCTCTGCAT CCGGAAAGTC ATTTTAACCA TCATGCAGTC GACCAGACGC
    AGAGATACAG CGGTGAGCAT CGAGTCCATG TGTGCCGATG ATCTGCTGTT GGTCATTCTG
    TATCTGCTCA TCAAGACAGA AATTCCCAAC TGGATGGCCA ATCTGAGTTA TATAAAGCAC
    TTCCGCTTTC GGAGCTCCAG CAAGGATGAG TTGAGTTACT GTCTGACGTC TTTCGAGGCC
    GCGGTCGAGT ATATCAGCCT GGGAAACCTC AAACACAACA CCGATGGGTC AGGAAACAGA
    CTGTCCTTCA GGCAGAAGGT GGACCTACTG TCCCAGAATG CAACACCCAT TGACCGGCTG
    TTTGAGCATA TTGTGAGCGG AAATGAGCAG GAGGTTCAGC GTTTGCTCAG TGAAAGCGAG
    AGTGAGGAGG ACGGCAGGAA GTTGTGTCAC CCTCTGTGTT CCTGTGACGC CTGCGACCTG
    CGCATCTCTG GGAGATTGAA TGATCCTTCC ATCGTCACTC CATTCTCTCG AGACGACAGA
    GGATACACTC CTTTACATGT TGCTGCTATT TGTGGTCAGT CTCAGATGAT TGATTTACTG
    GTGTCAAAGG GAGCAGTGGT AAACGCTACC GATTACCATG CACTCACACC GCTGCACCTC
    TCCTGCCAGA AGGGCTACCA GGGCGTCACG CTTCTCCTGC TGCATTATAA GGCCAACACT
    GATGCTCAGG ACAACAATGG AAACACTCCT CTACATCTGG CCTGCATGTA CGGACATGAA
    GACTGTGTGA AGGCGCTGGT GTACTTCGAT CTGCACTGCT GTCGGCTGAA CGTTCAGAAC
    GATAAAGGAG ACTCGCCGCT GCACATCGCC GCTCGCTGGG GATACGAGGG CATCATGGAG
    GTGCTGCTGG AGAACGGAGC GTCCACGCTC ATCCACAATA AAGCCAAGGA GACGCCGCTG
    CACTGCGCGC TCAACTCCAA GATTCTGTCA TTGTTGGAGC GCAGACACAA CGACTCCAGT
    ATAAGGGAAA GTGGATTCGA GTCTCCGAGC CGCTCTCCTC CTCCGTCAGA CGGCAGCAGC
    AGACGCTCCT CCATCTCCAG CTCATCCTCT CTGAGTATTG AAACCTCTCC GCTGGAGTCT
    GACCGCAGCC GGCACAGAGA GGTGGAGAAG CTGTTGAGAG CCGTGGCTGA CGGAGATGTG
    GAGATGGTGC GTTATCTCCT GGAGTGGCTC GATGAGGATC CAGATGAAGA GGAGTCTGCA
    GTGCCAGTCA AGACGGAGCT CTGCCATCCG CTGTGCCAGT GTGCCAACTG CGAACCCACA
    CAGAAGAAGG CAGCTCATCT TCATCCAGAC AGTGTGGGTG TAAACAGCAG CAGTGTTGAC
    GGCTTCACGC CGCTGCATGT GGCGGCGCTG CATGGACACA CACCTCTCAT CTCTCTGTTC
    ACACGCCACG GCGCAAACCT GAACGCTCGC AACAACCACA GCGCCACCCC ACTGCACCTG
    GCCTGCCAGA ACAACCACAC AGAGGTGGTC TGTGCTCTCC TGGAGTGTAA TGCCAAACTC
    AATAAGAAGG ATCATTATGG GAACACACCG CTCATTCAGG CCTGTCTGAA GGGGAATCTG
    GAGATCGCCG GCGTCCTGCT GGAGAGCGGT GCGCTGGTAA ATCTGGCCAA TAACCACGGG
    AACACGGGTC TGCATGAGGC TGTGAGAGGA GCAAACGTGT CGCTGGTGGA GCTGCTGCTG
    CACAGAGGAG CTTCAGTTCA CATCCGCAAC AACAGACAGA GAACATCGCT GGACTGCGCA
    CACGAGGCCG CCGCCAAGAA CTCAGAGATT TTGAGACTCC TTCAGAAAGC ATATGATGAA
    GCCCAGACGC AAAGGGCCAG AGCACCAGAG GGCATCATGG CAAGAGTTAA ACAGAGTCCT
    AACAATGGTG GACAGGATTT TGAGCACCAC AAACAGAGGA GTCTGAAGAG AACAGACAGA
    GCGGCAGCTA CAACAGCAGC AGCACAACAG ACTGCAGGGA GCGACAGACG GAGACTGACC
    CGAGGAGAAA CACTGGACAT CAGTGATCCA CAGCCTCCGA GCAGACCTCT GACCCGCAGC
    CACACCATCA GCCAAGACCT GAGTTCCAGC GTTCAGCCCG AAGAGCAGAT CAACGGTGGA
    AAACGGGAGG ATGAAGAAGA TCCGTCCGGT GCTGAAGCAG ATCCTGCAGA CACTGAGATG
    GACAGCGTGG TGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326424.1
    CDS171..3248
    Translation

    Target ORF information:

    RefSeq Version XM_021470749.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
    AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT
    GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT
    TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC
    CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC
    TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC
    CACATTCCTG AGGAGTTGGG TTCTGTTCCT GCTCCGTACT GTCTGAAAAA TATTGAGGAT
    GTGAGGGAGT TTTTGGGCCG CCATGCTGAA AAACTGGACA AGTTTGTTTC CTCCTTCTGT
    CTCTCCTTTA AAGAACAGGA GAGGAAAGGC CTACGCCATC ACATAGACTC GGTGAATACG
    CTTTACACCA GATGTCTTCA GTGTCTGCTG AGAGACTCTC GTCTGAAGCT TTTGGCGAGA
    CAGGAGATTC AGATGACTCT ACTCAAAGAA GCAGTAGAGA TGTACATCCA TCATGGCATC
    CATGATCTAC TCTTTAGTTA TGTGGGCACT CTTGAAGCTA GTCGGGATGC AGCGTTCAAC
    AAAACAACAC GGTCCTTGCA GGATCTGCAG CAGAAAGAGC TGGGAGTGAA GTCCGAGTTC
    AGTATTAATA TTCCTCGGGC GAAGCGTGAG CTGATTCAGC TGAACGTCTG CACGTCTCCA
    CAGCAGAAAC TCCTCTGCAT CCGGAAAGTC ATTTTAACCA TCATGCAGTC GACCAGACGC
    AGAGATACAG CGGTGAGCAT CGAGTCCATG TGTGCCGATG ATCTGCTGTT GGTCATTCTG
    TATCTGCTCA TCAAGACAGA AATTCCCAAC TGGATGGCCA ATCTGAGTTA TATAAAGCAC
    TTCCGCTTTC GGAGCTCCAG CAAGGATGAG TTGAGTTACT GTCTGACGTC TTTCGAGGCC
    GCGGTCGAGT ATATCAGCCT GGGAAACCTC AAACACAACA CCGATGGGTC AGGAAACAGA
    CTGTCCTTCA GGCAGAAGGT GGACCTACTG TCCCAGAATG CAACACCCAT TGACCGGCTG
    TTTGAGCATA TTGTGAGCGG AAATGAGCAG GAGGTTCAGC GTTTGCTCAG TGAAAGCGAG
    AGTGAGGAGG ACGGCAGGAA GTTGTGTCAC CCTCTGTGTT CCTGTGACGC CTGCGACCTG
    CGCATCTCTG GGAGATTGAA TGATCCTTCC ATCGTCACTC CATTCTCTCG AGACGACAGA
    GGATACACTC CTTTACATGT TGCTGCTATT TGTGGTCAGT CTCAGATGAT TGATTTACTG
    GTGTCAAAGG GAGCAGTGGT AAACGCTACC GATTACCATG CACTCACACC GCTGCACCTC
    TCCTGCCAGA AGGGCTACCA GGGCGTCACG CTTCTCCTGC TGCATTATAA GGCCAACACT
    GATGCTCAGG ACAACAATGG AAACACTCCT CTACATCTGG CCTGCATGTA CGGACATGAA
    GACTGTGTGA AGGCGCTGGT GTACTTCGAT CTGCACTGCT GTCGGCTGAA CGTTCAGAAC
    GATAAAGGAG ACTCGCCGCT GCACATCGCC GCTCGCTGGG GATACGAGGG CATCATGGAG
    GTGCTGCTGG AGAACGGAGC GTCCACGCTC ATCCACAATA AAGCCAAGGA GACGCCGCTG
    CACTGCGCGC TCAACTCCAA GATTCTGTCA TTGTTGGAGC GCAGACACAA CGACTCCAGT
    ATAAGGGAAA GTGGATTCGA GTCTCCGAGC CGCTCTCCTC CTCCGTCAGA CGGCAGCAGC
    AGACGCTCCT CCATCTCCAG CTCATCCTCT CTGAGTATTG AAACCTCTCC GCTGGAGTCT
    GACCGCAGCC GGCACAGAGA GGTGGAGAAG CTGTTGAGAG CCGTGGCTGA CGGAGATGTG
    GAGATGGTGC GTTATCTCCT GGAGTGGCTC GATGAGGATC CAGATGAAGA GGAGTCTGCA
    GTGCCAGTCA AGACGGAGCT CTGCCATCCG CTGTGCCAGT GTGCCAACTG CGAACCCACA
    CAGAAGAAGG CAGCTCATCT TCATCCAGAC AGTGTGGGTG TAAACAGCAG CAGTGTTGAC
    GGCTTCACGC CGCTGCATGT GGCGGCGCTG CATGGACACA CACCTCTCAT CTCTCTGTTC
    ACACGCCACG GCGCAAACCT GAACGCTCGC AACAACCACA GCGCCACCCC ACTGCACCTG
    GCCTGCCAGA ACAACCACAC AGAGGTGGTC TGTGCTCTCC TGGAGTGTAA TGCCAAACTC
    AATAAGAAGG ATCATTATGG GAACACACCG CTCATTCAGG CCTGTCTGAA GGGGAATCTG
    GAGATCGCCG GCGTCCTGCT GGAGAGCGGT GCGCTGGTAA ATCTGGCCAA TAACCACGGG
    AACACGGGTC TGCATGAGGC TGTGAGAGGA GCAAACGTGT CGCTGGTGGA GCTGCTGCTG
    CACAGAGGAG CTTCAGTTCA CATCCGCAAC AACAGACAGA GAACATCGCT GGACTGCGCA
    CACGAGGCCG CCGCCAAGAA CTCAGAGATT TTGAGACTCC TTCAGAAAGC ATATGATGAA
    GCCCAGACGC AAAGGGCCAG AGCACCAGAG GGCATCATGG CAAGAGTTAA ACAGAGTCCT
    AACAATGGTG GACAGGATTT TGAGCACCAC AAACAGAGGA GTCTGAAGAG AACAGACAGA
    GCGGCAGCTA CAACAGCAGC AGCACAACAG ACTGCAGGGA GCGACAGACG GAGACTGACC
    CGAGGAGAAA CACTGGACAT CAGTGATCCA CAGCCTCCGA GCAGACCTCT GACCCGCAGC
    CACACCATCA GCCAAGACCT GAGTTCCAGC GTTCAGCCCG AAGAGCAGAT CAACGGTGGA
    AAACGGGAGG ATGAAGAAGA TCCGTCCGGT GCTGAAGCAG ATCCTGCAGA CACTGAGATG
    GACAGCGTGG TGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57976
    Clone ID Related Accession (Same CDS sequence) XM_017354281.1
    Accession Version XM_017354281.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ankyrin repeat domain-containing protein 27 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2012 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
    TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG
    TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC
    CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA
    CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG
    GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT
    GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG
    AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT
    GCTGAGGAGT TGGGTTCTGT TCCTGCTCCG TACTGTCTGA AAAATATTGA GGATGTGAGG
    GAGTTTTTGG GCCGCCATGC TGAAAAACTG GACAAGTTTG TTTCCTCCTT CTGTCTCTCC
    TTTAAAGAAC AGGAGAGGAA AGGCCTACGC CATCACATAG ACTCGGTGAA TACGCTTTAC
    ACCAGATGTC TTCAGTGTCT GCTGAGAGAC TCTCGTCTGA AGCTTTTGGC GAGACAGGAG
    ATTCAGATGA CTCTACTCAA AGAAGCAGTA GAGATGTACA TCCATCATGG CATCCATGAT
    CTACTCTTTA GTTATGTGGG CACTCTTGAA GCTAGTCGGG ATGCAGCGTT CAACAAAACA
    ACACGGTCCT TGCAGGATCT GCAGCAGAAA GAGCTGGGAG TGAAGTCCGA GTTCAGTATT
    AATATTCCTC GGGCGAAGCG TGAGCTGATT CAGCTGAACG TCTGCACGTC TCCACAGCAG
    AAACTCCTCT GCATCCGGAA AGTCATTTTA ACCATCATGC AGTCGACCAG ACGCAGAGAT
    ACAGCGGTGA GCATCGAGTC CATGTGTGCC GATGATCTGC TGTTGGTCAT TCTGTATCTG
    CTCATCAAGA CAGAAATTCC CAACTGGATG GCCAATCTGA GTTATATAAA GCACTTCCGC
    TTTCGGAGCT CCAGCAAGGA TGAGTTGAGT TACTGTCTGA CGTCTTTCGA GGCCGCGGTC
    GAGTATATCA GCCTGGGAAA CCTCAAACAC AACACCGATG GGTCAGGAAA CAGACTGTCC
    TTCAGGCAGA AGGTGGACCT ACTGTCCCAG AATGCAACAC CCATTGACCG GCTGTTTGAG
    CATATTGTGA GCGGAAATGA GCAGGAGGTT CAGCGTTTGC TCAGTGAAAG CGAGAGTGAG
    GAGGACGGCA GGAAGTTGTG TCACCCTCTG TGTTCCTGTG ACGCCTGCGA CCTGCGCATC
    TCTGGGAGAT TGAATGATCC TTCCATCGTC ACTCCATTCT CTCGAGACGA CAGAGGATAC
    ACTCCTTTAC ATGTTGCTGC TATTTGTGGT CAGTCTCAGA TGATTGATTT ACTGGTGTCA
    AAGGGAGCAG TGGTAAACGC TACCGATTAC CATGCACTCA CACCGCTGCA CCTCTCCTGC
    CAGAAGGGCT ACCAGGGCGT CACGCTTCTC CTGCTGCATT ATAAGGCCAA CACTGATGCT
    CAGGACAACA ATGGAAACAC TCCTCTACAT CTGGCCTGCA TGTACGGACA TGAAGACTGT
    GTGAAGGCGC TGGTGTACTT CGATCTGCAC TGCTGTCGGC TGAACGTTCA GAACGATAAA
    GGAGACTCGC CGCTGCACAT CGCCGCTCGC TGGGGATACG AGGGCATCAT GGAGGTGCTG
    CTGGAGAACG GAGCGTCCAC GCTCATCCAC AATAAAGCCA AGGAGACGCC GCTGCACTGC
    GCGCTCAACT CCAAGATTCT GTCATTGTTG GAGCGCAGAC ACAACGACTC CAGTATAAGG
    GAAAGTGGAT TCGAGTCTCC GAGCCGCTCT CCTCCTCCGT CAGACGGCAG CAGCAGACGC
    TCCTCCATCT CCAGCTCATC CTCTCTGAGT ATTGAAACCT CTCCGCTGGA GTCTGACCGC
    AGCCGGCATA GAGAGGTGGA GAAGCTGTTG AGAGCCGTGG CTGACGGAGA TGTGGAGATG
    GTGCGTTATC TCCTGGAGTG GCTGGATGAG GATCCAGATG AAGAGGAGTC TGCAGTGCCA
    GTCAAGACGG AGCTCTGCCA TCCGCTGTGC CAGTGTGCCA ACTGCGAACC CACACAGAAG
    AAGGCAGCTC ATCTTCATCC AGACAGTGTG GGTGTGAACA GCAGCAGTGT TGACGGCTTT
    ACGCCGCTGC ATGTGGCGGC GCTGCATGGA CACACACCTC TCATCTCTCT GTTCACACAC
    CACGGCGCAA ACCTGAACGC TCGCAACAAC CACAGCGCCA CCCCACTGCA CCTGGCCTGC
    CAGAACAACC ACACAGAGGT GGTCTGTGCT CTCCTGGAGT GTAATGCCAA ACTCAATAAG
    AAGGATCATT ATGGGAACAC ACCGCTCATT CAGGCCTGTC TGAAGGGGAA TCTGGAGATC
    GCCGGCGTCC TGCTGGAGAG CGGTGCGCTG GTAAATCTGG CCAATAACCA CGGGAACACG
    GGTCTGCATG AGGCTGTGAG AGGAGCAAAC GTGCCGCTGG TGGAGCTGCT GCTGCACAGA
    GGAGCTTCAG TTCACATCCG CAACAACAGA CAGAGAACAG CGCTGGACTG CGCACACGAG
    GCCGCCGCCA AGAACTCAGA GATTTTGAGA CTCCTTCAGA AAGCATATGA TGAAGCCCAG
    ACGCAAAGGG CCAGAGCACC AGAGGGCATC ATGGCAAGAG TTAAACAGAG TCCTAACAAT
    GGTGGACAGG ATTTTGAGCA CCACAAACAG AGGAGTCTGA AGAGAACAGA CAGAGCGGCA
    GCTACAACAG CAGCAGCACA ACAGACTGCA GGGAGCGACA GACGGAGACT GACCCGAGGA
    GAAACACTGG ACATCAGTGA TCCACAGCCT CCGAGCAGAC CTCTGACCCG CAGCCACACC
    ATCAGCCAAG ACCTGAGTTC CAGCGTTCAG CCCGAAGAGC AGATCAACGG TGGAAAACGG
    GAGGATGAAG AAGATCCGTC CGGTGCTGAA GCAGATCCTG CAGACACTGA GATGGACAGC
    GTGGTGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209770.1
    CDS185..3376
    Translation

    Target ORF information:

    RefSeq Version XM_017354281.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354281.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCGTTCC TCTTCCTCAT TCGTCATTTG ATGGATTTAA AGATGAAATT GTACATGGTG 
    TCGAAAACTC TTCTTAACCT TCATAAATAT GAGCTGGAGT TTGGGACACA TATGGCTGTG
    TATGATGAGA ATATCCTGAA AAACCCTTTT TACCAGGCTC TGGAGAAACA CAGACCGGAC
    CTGTGCAGCC GGGTGGCAGA GCTCCATGGC ATTATCCTGG TACCGTGTTG TGGAAGTTTA
    CCAACGGGCT CTTTTACTGC AGCTCAGTTT GACGGATACG TTCTTCAACC TTCCGAGCAG
    GGTTACCAGA CTGTAGATGG AAAGGAAGTG TCTATAAACA ATAACCGGGT CCACCTGAGT
    GCAGGGTTGC CCAATCCTAT CTCTGTCCCC ATCCTGTTTG AGGAGACCTT CTACAATGAG
    AAAGAGCAGG CCTACAGTAT ACTGTGCATC GCACGGCCCT TCGAATCTGA CCACATTCCT
    GCTGAGGAGT TGGGTTCTGT TCCTGCTCCG TACTGTCTGA AAAATATTGA GGATGTGAGG
    GAGTTTTTGG GCCGCCATGC TGAAAAACTG GACAAGTTTG TTTCCTCCTT CTGTCTCTCC
    TTTAAAGAAC AGGAGAGGAA AGGCCTACGC CATCACATAG ACTCGGTGAA TACGCTTTAC
    ACCAGATGTC TTCAGTGTCT GCTGAGAGAC TCTCGTCTGA AGCTTTTGGC GAGACAGGAG
    ATTCAGATGA CTCTACTCAA AGAAGCAGTA GAGATGTACA TCCATCATGG CATCCATGAT
    CTACTCTTTA GTTATGTGGG CACTCTTGAA GCTAGTCGGG ATGCAGCGTT CAACAAAACA
    ACACGGTCCT TGCAGGATCT GCAGCAGAAA GAGCTGGGAG TGAAGTCCGA GTTCAGTATT
    AATATTCCTC GGGCGAAGCG TGAGCTGATT CAGCTGAACG TCTGCACGTC TCCACAGCAG
    AAACTCCTCT GCATCCGGAA AGTCATTTTA ACCATCATGC AGTCGACCAG ACGCAGAGAT
    ACAGCGGTGA GCATCGAGTC CATGTGTGCC GATGATCTGC TGTTGGTCAT TCTGTATCTG
    CTCATCAAGA CAGAAATTCC CAACTGGATG GCCAATCTGA GTTATATAAA GCACTTCCGC
    TTTCGGAGCT CCAGCAAGGA TGAGTTGAGT TACTGTCTGA CGTCTTTCGA GGCCGCGGTC
    GAGTATATCA GCCTGGGAAA CCTCAAACAC AACACCGATG GGTCAGGAAA CAGACTGTCC
    TTCAGGCAGA AGGTGGACCT ACTGTCCCAG AATGCAACAC CCATTGACCG GCTGTTTGAG
    CATATTGTGA GCGGAAATGA GCAGGAGGTT CAGCGTTTGC TCAGTGAAAG CGAGAGTGAG
    GAGGACGGCA GGAAGTTGTG TCACCCTCTG TGTTCCTGTG ACGCCTGCGA CCTGCGCATC
    TCTGGGAGAT TGAATGATCC TTCCATCGTC ACTCCATTCT CTCGAGACGA CAGAGGATAC
    ACTCCTTTAC ATGTTGCTGC TATTTGTGGT CAGTCTCAGA TGATTGATTT ACTGGTGTCA
    AAGGGAGCAG TGGTAAACGC TACCGATTAC CATGCACTCA CACCGCTGCA CCTCTCCTGC
    CAGAAGGGCT ACCAGGGCGT CACGCTTCTC CTGCTGCATT ATAAGGCCAA CACTGATGCT
    CAGGACAACA ATGGAAACAC TCCTCTACAT CTGGCCTGCA TGTACGGACA TGAAGACTGT
    GTGAAGGCGC TGGTGTACTT CGATCTGCAC TGCTGTCGGC TGAACGTTCA GAACGATAAA
    GGAGACTCGC CGCTGCACAT CGCCGCTCGC TGGGGATACG AGGGCATCAT GGAGGTGCTG
    CTGGAGAACG GAGCGTCCAC GCTCATCCAC AATAAAGCCA AGGAGACGCC GCTGCACTGC
    GCGCTCAACT CCAAGATTCT GTCATTGTTG GAGCGCAGAC ACAACGACTC CAGTATAAGG
    GAAAGTGGAT TCGAGTCTCC GAGCCGCTCT CCTCCTCCGT CAGACGGCAG CAGCAGACGC
    TCCTCCATCT CCAGCTCATC CTCTCTGAGT ATTGAAACCT CTCCGCTGGA GTCTGACCGC
    AGCCGGCATA GAGAGGTGGA GAAGCTGTTG AGAGCCGTGG CTGACGGAGA TGTGGAGATG
    GTGCGTTATC TCCTGGAGTG GCTGGATGAG GATCCAGATG AAGAGGAGTC TGCAGTGCCA
    GTCAAGACGG AGCTCTGCCA TCCGCTGTGC CAGTGTGCCA ACTGCGAACC CACACAGAAG
    AAGGCAGCTC ATCTTCATCC AGACAGTGTG GGTGTGAACA GCAGCAGTGT TGACGGCTTT
    ACGCCGCTGC ATGTGGCGGC GCTGCATGGA CACACACCTC TCATCTCTCT GTTCACACAC
    CACGGCGCAA ACCTGAACGC TCGCAACAAC CACAGCGCCA CCCCACTGCA CCTGGCCTGC
    CAGAACAACC ACACAGAGGT GGTCTGTGCT CTCCTGGAGT GTAATGCCAA ACTCAATAAG
    AAGGATCATT ATGGGAACAC ACCGCTCATT CAGGCCTGTC TGAAGGGGAA TCTGGAGATC
    GCCGGCGTCC TGCTGGAGAG CGGTGCGCTG GTAAATCTGG CCAATAACCA CGGGAACACG
    GGTCTGCATG AGGCTGTGAG AGGAGCAAAC GTGCCGCTGG TGGAGCTGCT GCTGCACAGA
    GGAGCTTCAG TTCACATCCG CAACAACAGA CAGAGAACAG CGCTGGACTG CGCACACGAG
    GCCGCCGCCA AGAACTCAGA GATTTTGAGA CTCCTTCAGA AAGCATATGA TGAAGCCCAG
    ACGCAAAGGG CCAGAGCACC AGAGGGCATC ATGGCAAGAG TTAAACAGAG TCCTAACAAT
    GGTGGACAGG ATTTTGAGCA CCACAAACAG AGGAGTCTGA AGAGAACAGA CAGAGCGGCA
    GCTACAACAG CAGCAGCACA ACAGACTGCA GGGAGCGACA GACGGAGACT GACCCGAGGA
    GAAACACTGG ACATCAGTGA TCCACAGCCT CCGAGCAGAC CTCTGACCCG CAGCCACACC
    ATCAGCCAAG ACCTGAGTTC CAGCGTTCAG CCCGAAGAGC AGATCAACGG TGGAAAACGG
    GAGGATGAAG AAGATCCGTC CGGTGCTGAA GCAGATCCTG CAGACACTGA GATGGACAGC
    GTGGTGTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57977
    Clone ID Related Accession (Same CDS sequence) XM_002666959.5 , XM_017354282.1
    Accession Version XM_002666959.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ankyrin repeat domain-containing protein 27 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002666959.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2012 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
    AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT
    GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT
    TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC
    CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC
    TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC
    CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG
    GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC
    TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT
    ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG
    AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC
    ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC
    AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG
    TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT
    CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA
    CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT
    CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG
    CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG
    GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC
    AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG
    CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC
    GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC
    CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC
    AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA
    CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC
    CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC
    ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT
    GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG
    AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG
    GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG
    CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC
    AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC
    AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG
    TCTGACCGCA GCCGGCATAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT
    GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTGGATGAGG ATCCAGATGA AGAGGAGTCT
    GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC
    ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTGAACAG CAGCAGTGTT
    GACGGCTTTA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG
    TTCACACACC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC
    CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA
    CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT
    CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC
    GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGCCGCTGGT GGAGCTGCTG
    CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACAGC GCTGGACTGC
    GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT
    GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT
    CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC
    AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG
    ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC
    AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT
    GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG
    ATGGACAGCG TGGTGTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002667005.4
    CDS142..3222
    Translation

    Target ORF information:

    RefSeq Version XM_002666959.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002666959.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
    AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT
    GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT
    TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC
    CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC
    TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC
    CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG
    GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC
    TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT
    ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG
    AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC
    ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC
    AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG
    TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT
    CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA
    CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT
    CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG
    CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG
    GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC
    AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG
    CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC
    GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC
    CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC
    AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA
    CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC
    CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC
    ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT
    GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG
    AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG
    GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG
    CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC
    AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC
    AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG
    TCTGACCGCA GCCGGCATAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT
    GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTGGATGAGG ATCCAGATGA AGAGGAGTCT
    GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC
    ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTGAACAG CAGCAGTGTT
    GACGGCTTTA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG
    TTCACACACC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC
    CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA
    CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT
    CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC
    GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGCCGCTGGT GGAGCTGCTG
    CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACAGC GCTGGACTGC
    GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT
    GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT
    CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC
    AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG
    ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC
    AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT
    GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG
    ATGGACAGCG TGGTGTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57977
    Clone ID Related Accession (Same CDS sequence) XM_002666959.5 , XM_017354282.1
    Accession Version XM_017354282.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product ankyrin repeat domain-containing protein 27 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) and transcript sequence (GDQQ01008931.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2012 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
    AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT
    GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT
    TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC
    CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC
    TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC
    CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG
    GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC
    TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT
    ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG
    AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC
    ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC
    AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG
    TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT
    CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA
    CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT
    CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG
    CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG
    GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC
    AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG
    CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC
    GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC
    CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC
    AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA
    CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC
    CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC
    ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT
    GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG
    AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG
    GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG
    CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC
    AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC
    AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG
    TCTGACCGCA GCCGGCATAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT
    GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTGGATGAGG ATCCAGATGA AGAGGAGTCT
    GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC
    ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTGAACAG CAGCAGTGTT
    GACGGCTTTA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG
    TTCACACACC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC
    CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA
    CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT
    CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC
    GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGCCGCTGGT GGAGCTGCTG
    CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACAGC GCTGGACTGC
    GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT
    GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT
    CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC
    AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG
    ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC
    AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT
    GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG
    ATGGACAGCG TGGTGTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209771.1
    CDS63..3143
    Translation

    Target ORF information:

    RefSeq Version XM_017354282.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ankyrin repeat domain 27 (VPS9 domain) (ankrd27), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354282.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCTGTGT ATGATGAGAA TATCCTGAAA AACCCTTTTT ACCAGGCTCT GGAGAAACAC 
    AGACCGGACC TGTGCAGCCG GGTGGCAGAG CTCCATGGCA TTATCCTGGT ACCGTGTTGT
    GGAAGTTTAC CAACGGGCTC TTTTACTGCA GCTCAGTTTG ACGGATACGT TCTTCAACCT
    TCCGAGCAGG GTTACCAGAC TGTAGATGGA AAGGAAGTGT CTATAAACAA TAACCGGGTC
    CACCTGAGTG CAGGGTTGCC CAATCCTATC TCTGTCCCCA TCCTGTTTGA GGAGACCTTC
    TACAATGAGA AAGAGCAGGC CTACAGTATA CTGTGCATCG CACGGCCCTT CGAATCTGAC
    CACATTCCTG CTGAGGAGTT GGGTTCTGTT CCTGCTCCGT ACTGTCTGAA AAATATTGAG
    GATGTGAGGG AGTTTTTGGG CCGCCATGCT GAAAAACTGG ACAAGTTTGT TTCCTCCTTC
    TGTCTCTCCT TTAAAGAACA GGAGAGGAAA GGCCTACGCC ATCACATAGA CTCGGTGAAT
    ACGCTTTACA CCAGATGTCT TCAGTGTCTG CTGAGAGACT CTCGTCTGAA GCTTTTGGCG
    AGACAGGAGA TTCAGATGAC TCTACTCAAA GAAGCAGTAG AGATGTACAT CCATCATGGC
    ATCCATGATC TACTCTTTAG TTATGTGGGC ACTCTTGAAG CTAGTCGGGA TGCAGCGTTC
    AACAAAACAA CACGGTCCTT GCAGGATCTG CAGCAGAAAG AGCTGGGAGT GAAGTCCGAG
    TTCAGTATTA ATATTCCTCG GGCGAAGCGT GAGCTGATTC AGCTGAACGT CTGCACGTCT
    CCACAGCAGA AACTCCTCTG CATCCGGAAA GTCATTTTAA CCATCATGCA GTCGACCAGA
    CGCAGAGATA CAGCGGTGAG CATCGAGTCC ATGTGTGCCG ATGATCTGCT GTTGGTCATT
    CTGTATCTGC TCATCAAGAC AGAAATTCCC AACTGGATGG CCAATCTGAG TTATATAAAG
    CACTTCCGCT TTCGGAGCTC CAGCAAGGAT GAGTTGAGTT ACTGTCTGAC GTCTTTCGAG
    GCCGCGGTCG AGTATATCAG CCTGGGAAAC CTCAAACACA ACACCGATGG GTCAGGAAAC
    AGACTGTCCT TCAGGCAGAA GGTGGACCTA CTGTCCCAGA ATGCAACACC CATTGACCGG
    CTGTTTGAGC ATATTGTGAG CGGAAATGAG CAGGAGGTTC AGCGTTTGCT CAGTGAAAGC
    GAGAGTGAGG AGGACGGCAG GAAGTTGTGT CACCCTCTGT GTTCCTGTGA CGCCTGCGAC
    CTGCGCATCT CTGGGAGATT GAATGATCCT TCCATCGTCA CTCCATTCTC TCGAGACGAC
    AGAGGATACA CTCCTTTACA TGTTGCTGCT ATTTGTGGTC AGTCTCAGAT GATTGATTTA
    CTGGTGTCAA AGGGAGCAGT GGTAAACGCT ACCGATTACC ATGCACTCAC ACCGCTGCAC
    CTCTCCTGCC AGAAGGGCTA CCAGGGCGTC ACGCTTCTCC TGCTGCATTA TAAGGCCAAC
    ACTGATGCTC AGGACAACAA TGGAAACACT CCTCTACATC TGGCCTGCAT GTACGGACAT
    GAAGACTGTG TGAAGGCGCT GGTGTACTTC GATCTGCACT GCTGTCGGCT GAACGTTCAG
    AACGATAAAG GAGACTCGCC GCTGCACATC GCCGCTCGCT GGGGATACGA GGGCATCATG
    GAGGTGCTGC TGGAGAACGG AGCGTCCACG CTCATCCACA ATAAAGCCAA GGAGACGCCG
    CTGCACTGCG CGCTCAACTC CAAGATTCTG TCATTGTTGG AGCGCAGACA CAACGACTCC
    AGTATAAGGG AAAGTGGATT CGAGTCTCCG AGCCGCTCTC CTCCTCCGTC AGACGGCAGC
    AGCAGACGCT CCTCCATCTC CAGCTCATCC TCTCTGAGTA TTGAAACCTC TCCGCTGGAG
    TCTGACCGCA GCCGGCATAG AGAGGTGGAG AAGCTGTTGA GAGCCGTGGC TGACGGAGAT
    GTGGAGATGG TGCGTTATCT CCTGGAGTGG CTGGATGAGG ATCCAGATGA AGAGGAGTCT
    GCAGTGCCAG TCAAGACGGA GCTCTGCCAT CCGCTGTGCC AGTGTGCCAA CTGCGAACCC
    ACACAGAAGA AGGCAGCTCA TCTTCATCCA GACAGTGTGG GTGTGAACAG CAGCAGTGTT
    GACGGCTTTA CGCCGCTGCA TGTGGCGGCG CTGCATGGAC ACACACCTCT CATCTCTCTG
    TTCACACACC ACGGCGCAAA CCTGAACGCT CGCAACAACC ACAGCGCCAC CCCACTGCAC
    CTGGCCTGCC AGAACAACCA CACAGAGGTG GTCTGTGCTC TCCTGGAGTG TAATGCCAAA
    CTCAATAAGA AGGATCATTA TGGGAACACA CCGCTCATTC AGGCCTGTCT GAAGGGGAAT
    CTGGAGATCG CCGGCGTCCT GCTGGAGAGC GGTGCGCTGG TAAATCTGGC CAATAACCAC
    GGGAACACGG GTCTGCATGA GGCTGTGAGA GGAGCAAACG TGCCGCTGGT GGAGCTGCTG
    CTGCACAGAG GAGCTTCAGT TCACATCCGC AACAACAGAC AGAGAACAGC GCTGGACTGC
    GCACACGAGG CCGCCGCCAA GAACTCAGAG ATTTTGAGAC TCCTTCAGAA AGCATATGAT
    GAAGCCCAGA CGCAAAGGGC CAGAGCACCA GAGGGCATCA TGGCAAGAGT TAAACAGAGT
    CCTAACAATG GTGGACAGGA TTTTGAGCAC CACAAACAGA GGAGTCTGAA GAGAACAGAC
    AGAGCGGCAG CTACAACAGC AGCAGCACAA CAGACTGCAG GGAGCGACAG ACGGAGACTG
    ACCCGAGGAG AAACACTGGA CATCAGTGAT CCACAGCCTC CGAGCAGACC TCTGACCCGC
    AGCCACACCA TCAGCCAAGA CCTGAGTTCC AGCGTTCAGC CCGAAGAGCA GATCAACGGT
    GGAAAACGGG AGGATGAAGA AGATCCGTCC GGTGCTGAAG CAGATCCTGC AGACACTGAG
    ATGGACAGCG TGGTGTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.