prkci cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prkci
Entrez Gene ID	117507
Full Name	protein kinase C, iota
Synonyms	aPKC[l],hal,has
General protein information	Preferred Names protein kinase C, iota Names protein kinase C iota type aPKC-lambda/iota atypical protein kinase C-lambda/iota heart and soul protein nPKC-iota
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131855.2	NP_571930.2	protein kinase C iota type

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PRKCI	NP_001026490.1
Drosophila melanogaster (fruit fly)	aPKC	NP_001036541.1
Homo sapiens (human)	PRKCI	NP_002731.4
Mus musculus (house mouse)	Prkci	NP_032883.2
Pan troglodytes (chimpanzee)	PRKCI	XP_526377.3
	PRKCI	XP_001089400.2
Bos taurus (cattle)	PRKCI	NP_001192884.1
Rattus norvegicus (Norway rat)	Prkci	NP_114448.1
Danio rerio (zebrafish)	prkci	NP_571930.2
Caenorhabditis elegans (roundworm)	pkc-3	NP_495011.1
Xenopus tropicalis (tropical clawed frog)	prkci	NP_001012707.1
Canis lupus familiaris (dog)	PRKCI	XP_535855.3

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina.
Icha J, Kunath C, Rocha-Martins M, Norden C
The Journal of cell biology215(2)259-275(2016 Oct)

aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis.
Raman R, Damle I, Rote R, Banerjee S, Dingare C, Sonawane M
Nature communications711643(2016 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta.
Title: Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta.

a loss of zBves affects the proteins involved in the pathway of the PAR junctional complex, especially aPKC, and both aPKC and Bves are indispensable to claudin expression.
Title: Blood vessel epicardial substance (Bves) regulates epidermal tight junction integrity through atypical protein kinase C.

These results suggest that the aPKC cell autonomously controls the Golgi localization and thereby regulates the specification of the primary dendrite of Purkinje cells.
Title: Atypical protein kinase C regulates primary dendrite specification of cerebellar Purkinje cells by localizing Golgi apparatus.

PrkCi function and planar divisions are necessary for asymmetric, self-renewing division of spinal cord precursors.
Title: Apical polarity protein PrkCi is necessary for maintenance of spinal cord precursors in zebrafish.

Heart and soul/PRKCi and nagie oko/Mpp5 regulate myocardial coherence and remodeling during cardiac morphogenesis.
Title: Heart and soul/PRKCi and nagie oko/Mpp5 regulate myocardial coherence and remodeling during cardiac morphogenesis.

The following prkci gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prkci cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01395	NM_131855.2 Latest version!	Danio rerio protein kinase C, iota (prkci), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prkci ( NM_131855.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571930.2 Danio rerio protein kinase C, iota (prkci), mRNA.

Price & Availability↑

CloneID	ODa01395
Clone ID Related Accession (Same CDS sequence)	NM_131855.2
Accession Version	NM_131855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Danio rerio(zebrafish)
Product	protein kinase C iota type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC075736.1, EB966090.1, EB880353.1 and BX511231.4. On Mar 20, 2007 this sequence version replaced NM_131855.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF390109.1, BC047164.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCACGC TGCGGGACAG CACCATGTCC CACCCCGGAG AAAACCCGCA CCAAGTCCGG 
GTAAAAGCCT ACTACAGAGG GGACATCATG ATCACACATT TTGAGCCTTC GATCTCCTAT 
GAGGGACTCT GCAATGAGGT GCGGGATATG TGCTCCATGG ACAATGACCA GCTCTTCACC 
ATGAAATGGA TTGATGAGGA AGGGGATCCA TGCACCGTTT CTTCTCAGCT GGAGCTGGAG 
GAGGCCTTGC GTCTATATGA ACTCAACAAA GACTCGGAGC TCATTATTCA CGTGTTTCCT 
TGTGTCCCTG AAAAACCTGG CATGCCCTGT CCTGGAGAAG ACAAGTCTAT ATACCGGCGG 
GGAGCTCGAC GTTGGAGGAA ACTCTACTAT GCCACTGGAC ATGCGTTTCA GGCCAAACGC 
TTTAACAGGC GTGCTCATTG TGCCATCTGC ACAGATCGTA TCTGGGGTCT GGGCAGGCAG 
GGATACAAGT GTATCAACTG TAAGCTTCTG GTGCATAAGA AATGCCATAA GCTGGTCACA 
GTAGAATGTG GTAGACAGGT AATACAGGAC CCAATGATCG GAAGAATCGA TCCAGGGTCG 
ACTCATCCAG AGCACCCAGA TCAAGTTCTG GGCAAAAAGA ACTCAACAGA AAGCATCAAT 
CATGAGGGAG AGGAGCATGA GGCTGTGGGC AGTCGGGAAT CAGGAAAAGC GGTGTCCAGT 
TTGGGTCTAA TAGACTTTGA CCTGCTGCGA GTGATTGGCA GGGGCAGCTA TGCCAAAGTT 
CTGCTGGTGC GTCTCAAAAA GACAGAACGC ATCTATGCCA TGAAGGTGGT GAAGAAGGAG 
CTGGTCAACG ATGACGAGGA TATTGACTGG GTTCAGACTG AAAAGCATGT GTTTGAGCAG 
GCTTCAAACC ATCCCTTCCT TGTGGGACTT CACTCCTGCT TCCAGACGGA GAGCAGACTG 
TTCTTTGTAA TCGAGTATGT GAATGGAGGG GATCTCATGT TCCACATGCA GCGGCAGAGG 
AAACTTCCGG AAGAGCACGC CAGGTTTTAC TCTGCAGAGA TCAGTCTTGC CTTGAACTAC 
CTCCATGAGC GTGGCATTAT TTACAGGGAC CTGAAACTGG ACAATGTTCT GCTGGATTCA 
GAGGGACACA TCAAACTCAC CGATTACGGC ATGTGTAAGG AGGGACTGAG ACCAGGAGAT 
ACAACCAGCA CTTTCTGTGG AACTCCCAAT TACATTGCAC CAGAGATTCT GAGAGGAGAA 
GACTATGGTT TTAGTGTGGA CTGGTGGGCT CTGGGCGTCC TGATGTTTGA GATGATGGCT 
GGAAGATCTC CCTTCGACAT AGTCGGCAGC TCTGATAACC CTGACCAAAA CACAGAGGAT 
TATCTTTTCC AAGTCATTTT GGAGAAGCAG ATCAGAATTC CCAGATCGTT ATCGGTCAAA 
GCCGCAAGCG TGCTGAAGGG ATTCCTCAAC AAGGAGTCGA AGGAACGGCT GGGATGTCAT 
CCTCAGACAG GCTTCGCAGA CATCATGGCC CATCCTTTTT TCCGAAATGT AGACTGGGAT 
CTTATGGAGC AGAAGCAAGT AGTTCCACCG TTCAAACCTA ACATCTCGGG CGAGTTTGGT 
CTGGATAACT TTGATGCCCA GTTCACCAAC GAGCCCATTC AGCTCACGCC TGACGATGAT 
GATGCTGTAA AGAAGATCGA CCAGTCTGAG TTTGAAGGCT TCGAGTACAT CAACCCTCTG 
CTGATGTCTG CGGAGGAGTG TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571930.2
CDS	254..2020
Translation	MPTLRDSTMSHPGENPHQVRVKAYYRGDIMITHFEPSISYEGLCNEVRDMCSMDNDQLFTMKWIDEEGDPCTVSSQLELEEALRLYELNKDSELIIHVFPCVPEKPGMPCPGEDKSIYRRGARRWRKLYYATGHAFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTVECGRQVIQDPMIGRIDPGSTHPEHPDQVLGKKNSTESINHEGEEHEAVGSRESGKAVSSLGLIDFDLLRVIGRGSYAKVLLVRLKKTERIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKGFLNKESKERLGCHPQTGFADIMAHPFFRNVDWDLMEQKQVVPPFKPNISGEFGLDNFDAQFTNEPIQLTPDDDDAVKKIDQSEFEGFEYINPLLMSAEECV

Target ORF information:

RefSeq Version	NM_131855.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase C, iota (prkci), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131855.2

ORF Insert Sequence:

ATGCCCACGC TGCGGGACAG CACCATGTCC CACCCCGGAG AAAACCCGCA CCAAGTCCGG 
GTAAAAGCCT ACTACAGAGG GGACATCATG ATCACACATT TTGAGCCTTC GATCTCCTAT 
GAGGGACTCT GCAATGAGGT GCGGGATATG TGCTCCATGG ACAATGACCA GCTCTTCACC 
ATGAAATGGA TTGATGAGGA AGGGGATCCA TGCACCGTTT CTTCTCAGCT GGAGCTGGAG 
GAGGCCTTGC GTCTATATGA ACTCAACAAA GACTCGGAGC TCATTATTCA CGTGTTTCCT 
TGTGTCCCTG AAAAACCTGG CATGCCCTGT CCTGGAGAAG ACAAGTCTAT ATACCGGCGG 
GGAGCTCGAC GTTGGAGGAA ACTCTACTAT GCCACTGGAC ATGCGTTTCA GGCCAAACGC 
TTTAACAGGC GTGCTCATTG TGCCATCTGC ACAGATCGTA TCTGGGGTCT GGGCAGGCAG 
GGATACAAGT GTATCAACTG TAAGCTTCTG GTGCATAAGA AATGCCATAA GCTGGTCACA 
GTAGAATGTG GTAGACAGGT AATACAGGAC CCAATGATCG GAAGAATCGA TCCAGGGTCG 
ACTCATCCAG AGCACCCAGA TCAAGTTCTG GGCAAAAAGA ACTCAACAGA AAGCATCAAT 
CATGAGGGAG AGGAGCATGA GGCTGTGGGC AGTCGGGAAT CAGGAAAAGC GGTGTCCAGT 
TTGGGTCTAA TAGACTTTGA CCTGCTGCGA GTGATTGGCA GGGGCAGCTA TGCCAAAGTT 
CTGCTGGTGC GTCTCAAAAA GACAGAACGC ATCTATGCCA TGAAGGTGGT GAAGAAGGAG 
CTGGTCAACG ATGACGAGGA TATTGACTGG GTTCAGACTG AAAAGCATGT GTTTGAGCAG 
GCTTCAAACC ATCCCTTCCT TGTGGGACTT CACTCCTGCT TCCAGACGGA GAGCAGACTG 
TTCTTTGTAA TCGAGTATGT GAATGGAGGG GATCTCATGT TCCACATGCA GCGGCAGAGG 
AAACTTCCGG AAGAGCACGC CAGGTTTTAC TCTGCAGAGA TCAGTCTTGC CTTGAACTAC 
CTCCATGAGC GTGGCATTAT TTACAGGGAC CTGAAACTGG ACAATGTTCT GCTGGATTCA 
GAGGGACACA TCAAACTCAC CGATTACGGC ATGTGTAAGG AGGGACTGAG ACCAGGAGAT 
ACAACCAGCA CTTTCTGTGG AACTCCCAAT TACATTGCAC CAGAGATTCT GAGAGGAGAA 
GACTATGGTT TTAGTGTGGA CTGGTGGGCT CTGGGCGTCC TGATGTTTGA GATGATGGCT 
GGAAGATCTC CCTTCGACAT AGTCGGCAGC TCTGATAACC CTGACCAAAA CACAGAGGAT 
TATCTTTTCC AAGTCATTTT GGAGAAGCAG ATCAGAATTC CCAGATCGTT ATCGGTCAAA 
GCCGCAAGCG TGCTGAAGGG ATTCCTCAAC AAGGAGTCGA AGGAACGGCT GGGATGTCAT 
CCTCAGACAG GCTTCGCAGA CATCATGGCC CATCCTTTTT TCCGAAATGT AGACTGGGAT 
CTTATGGAGC AGAAGCAAGT AGTTCCACCG TTCAAACCTA ACATCTCGGG CGAGTTTGGT 
CTGGATAACT TTGATGCCCA GTTCACCAAC GAGCCCATTC AGCTCACGCC TGACGATGAT 
GATGCTGTAA AGAAGATCGA CCAGTCTGAG TTTGAAGGCT TCGAGTACAT CAACCCTCTG 
CTGATGTCTG CGGAGGAGTG TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina.
The Journal of cell biology215(2)259-275(2016 Oct)
Icha J,Kunath C,Rocha-Martins M,Norden C

aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis.
Nature communications711643(2016 Jun)
Raman R,Damle I,Rote R,Banerjee S,Dingare C,Sonawane M

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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prkci cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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