Prkci cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkci
Entrez Gene ID	84006
Full Name	protein kinase C, iota
Synonyms	Pkcl
General protein information	Preferred Names protein kinase C, iota Names protein kinase C iota type aPKC-lambda/iota atypical protein kinase C-lambda/iota nPKC-iota protein kinase C, lambda
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q24
Summary	member of the atypical subgroup of PKCs that do not contain the calcium binding domain and possess only one cysteine-rich region; plays a critical role in controlling cell growth and cell survival; enhances Rab2-mediated vesicle budding [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_032059.1	NP_114448.1	protein kinase C iota type

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PRKCI	NP_001026490.1
Drosophila melanogaster (fruit fly)	aPKC	NP_001036541.1
Homo sapiens (human)	PRKCI	NP_002731.4
Mus musculus (house mouse)	Prkci	NP_032883.2
Pan troglodytes (chimpanzee)	PRKCI	XP_526377.3
	PRKCI	XP_001089400.2
Bos taurus (cattle)	PRKCI	NP_001192884.1
Rattus norvegicus (Norway rat)	Prkci	NP_114448.1
Danio rerio (zebrafish)	prkci	NP_571930.2
Caenorhabditis elegans (roundworm)	pkc-3	NP_495011.1
Xenopus tropicalis (tropical clawed frog)	prkci	NP_001012707.1
Canis lupus familiaris (dog)	PRKCI	XP_535855.3

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of microtubule-associated motors drives intermediate filament network polarization.
Leduc C, Etienne-Manneville S
The Journal of cell biology216(6)1689-1703(2017 Jun)

Deletion of Protein Kinase C ? in POMC Neurons Predisposes to Diet-Induced Obesity.
Dorfman MD, Krull JE, Scarlett JM, Guyenet SJ, Sajan MP, Damian V, Nguyen HT, Leitges M, Morton GJ, Farese RV, Schwartz MW, Thaler JP
Diabetes66(4)920-934(2017 04)

Distinct Roles of PKC?/? and PKM? in the Initiation and Maintenance of Hippocampal Long-Term Potentiation and Memory.
Wang S, Sheng T, Ren S, Tian T, Lu W
Cell reports16(7)1954-61(2016 08)

PKC? is critical in AMPA receptor phosphorylation and synaptic incorporation during LTP.
Ren SQ, Yan JZ, Zhang XY, Bu YF, Pan WW, Yao W, Tian T, Lu W
The EMBO journal32(10)1365-80(2013 May)

FGF21 suppresses hepatic glucose production through the activation of atypical protein kinase C?/?.
Kong LJ, Feng W, Wright M, Chen Y, Dallas-yang Q, Zhou YP, Berger JP
European journal of pharmacology702(1-3)302-8(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Polarity signaling via CDC42/atypical protein kinase C can affect the dynamic turnover of the intermediate filament network to promote the polarization of the network itself.
Title: Regulation of microtubule-associated motors drives intermediate filament network polarization.

Acute inhibition of CNS aPKC activity increases food intake and glucose intolerance. Chronic inactivation of CNS aPKC activity increases body weight and food intake during high fat diet feeding.
Title: Deletion of Protein Kinase C ? in POMC Neurons Predisposes to Diet-Induced Obesity.

PKMzeta, but not PKCiota, knock-down disrupted previously established long-time memory. PKCiota and PKMz have distinct functions in long-term potentiation and memory, with PKMzeta playing a specific role in memory maintenance.
Title: Distinct Roles of PKC?/? and PKM? in the Initiation and Maintenance of Hippocampal Long-Term Potentiation and Memory.

PKClambda is critical in AMPA receptor phosphorylation and synaptic incorporation during long-term potentiation.
Title: PKC? is critical in AMPA receptor phosphorylation and synaptic incorporation during LTP.

FGF21 inhibits hepatic glucose production by the PI3K-dependent activation of protein kinase c iota.
Title: FGF21 suppresses hepatic glucose production through the activation of atypical protein kinase C?/?.

The following Prkci gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkci cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13608	NM_032059.1 Latest version!	Rattus norvegicus protein kinase C, iota (Prkci), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$349.30 $499.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkci ( NM_032059.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114448.1 Rattus norvegicus protein kinase C, iota (Prkci), mRNA.

Price & Availability↑

CloneID	ORa13608
Clone ID Related Accession (Same CDS sequence)	NM_032059.1
Accession Version	NM_032059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C iota type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07009904.1 and AABR07009903.1. On or before Sep 6, 2008 this sequence version replaced XM_001064601.1, XM_342223.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU517502.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGACCC AGAGGGACAG CAGCACCATG TCTCACACGG TCGCGTGCGG CGGCGGCGGG 
GACCATTCCC ACCAGGTCCG GGTGAAAGCC TACTACCGCG GGGATATTAT GATAACACAC 
TTCGAGCCTT CCATCTCCTT TGAAGGACTT TGCAGTGAGG TTCGAGATAT GTGTTCTTTT 
GACAATGAGC AGCCATTCAC CATGAAATGG ATAGATGAGG AAGGAGACCC GTGCACAGTG 
TCTTCTCAGT TGGAGTTGGA AGAGGCTTTC AGGCTGTATG AGTTGAACAA GGATTCTGAA 
CTCCTGATCC ACGTGTTCCC GTGTGTACCA GAGCGTCCTG GAATGCCTTG CCCAGGGGAA 
GACAAGTCCA TTTACCGCAG AGGGGCGCGC AGGTGGAGAA AGCTGTATTG TGCAAATGGC 
CACACTTTTC AAGCCAAACG CTTTAACAGG CGTGCCCACT GTGCCATCTG CACAGACAGG 
ATCTGGGGAC TTGGACGACA AGGATATAAA TGCATCAACT GCAAACTGCT GGTTCATAAG 
AAGTGCCACA AGCTTGTCAC AATTGAGTGT GGGCGGCATT CTTTGCCACC GGAACCCATG 
ATGCCAATGG ACCAGTCATC CATGCACCCA GACCACACAC AGACAGTAAT TCCATATAAT 
CCTTCAAGTC ATGAGAGTTT GGACCAAGTT GGTGAAGAAA AGGAGGCAAT GAACACCAGG 
GAGAGTGGGA AGGCGTCATC AAGCTTAGGT CTCCAGGATT TCGATTTGCT TCGAGTTATA 
GGGAGAGGAA GTTACGCCAA AGTACTGCTG GTTCGATTAA AAAAGACAGA TCGCATTTAT 
GCAATGAAGG TTGTGAAGAA AGAGCTCGTC AATGACGATG AGGATATTGA TTGGGTACAG 
ACAGAAAAGC ATGTGTTTGA GCAGGCGTCC AATCACCCTT TCCTTGTTGG TCTGCATTCC 
TGCTTCCAGA CAGAAAGCAG GCTGTTTTTT GTCATAGAAT ATGTGAATGG AGGGGATCTC 
ATGTTTCATA TGCAGCGGCA AAGAAAACTT CCTGAAGAAC ATGCCAGGTT TTACTCAGCA 
GAAATCAGTC TAGCACTAAA TTATCTTCAT GAGCGAGGGA TAATTTATAG AGATTTGAAG 
TTGGACAATG TACTGCTGGA CTCTGAAGGA CACATCAAAC TCACTGACTA CGGCATGTGT 
AAGGAAGGAT TACGGCCCGG AGATACAACC AGCACCTTCT GTGGGACTCC CAATTACATT 
GCTCCTGAGA TCTTAAGAGG AGAAGACTAT GGCTTCAGCG TTGACTGGTG GGCTCTTGGA 
GTACTCATGT TTGAGATGAT GGCGGGAAGG TCTCCATTTG ATATCGTTGG GAGCTCTGAC 
AATCCTGACC AAAACACAGA GGATTATCTG TTCCAAGTCA TTTTGGAGAA GCAGATTCGC 
ATACCGCGCT CCCTGTCTGT GAAAGCAGCA AGTGTGCTGA AGAGTTTCCT CAACAAGGAC 
CCAAAGGAAC GATTGGGTTG TCACCCTCAA ACTGGATTTG CTGACATCCA AGGACACCCA 
TTCTTCCGTA ATGTGGATTG GGACATGATG GAGCAGAAGC AAGTGGTTCC GCCCTTTAAA 
CCAAACATTT CTGGAGAATT TGGTTTGGAT AACTTTGACT CCCAGTTTAC CAACGAACCA 
GTCCAGCTCA CTCCAGATGA TGATGACATC GTGAGGAAGA TTGATCAGTC TGAATTTGAA 
GGTTTCGAGT ATATCAACCC TCTCTTGATG TCTGCAGAAG AGTGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114448.1
CDS	142..1932
Translation	MPTQRDSSTMSHTVACGGGGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCSEVRDMCSFDNEQPFTMKWIDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPPEPMMPMDQSSMHPDHTQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV

Target ORF information:

RefSeq Version	NM_032059.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, iota (Prkci), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032059.1

ORF Insert Sequence:

ATGCCGACCC AGAGGGACAG CAGCACCATG TCTCACACGG TCGCGTGCGG CGGCGGCGGG 
GACCATTCCC ACCAGGTCCG GGTGAAAGCC TACTACCGCG GGGATATTAT GATAACACAC 
TTCGAGCCTT CCATCTCCTT TGAAGGACTT TGCAGTGAGG TTCGAGATAT GTGTTCTTTT 
GACAATGAGC AGCCATTCAC CATGAAATGG ATAGATGAGG AAGGAGACCC GTGCACAGTG 
TCTTCTCAGT TGGAGTTGGA AGAGGCTTTC AGGCTGTATG AGTTGAACAA GGATTCTGAA 
CTCCTGATCC ACGTGTTCCC GTGTGTACCA GAGCGTCCTG GAATGCCTTG CCCAGGGGAA 
GACAAGTCCA TTTACCGCAG AGGGGCGCGC AGGTGGAGAA AGCTGTATTG TGCAAATGGC 
CACACTTTTC AAGCCAAACG CTTTAACAGG CGTGCCCACT GTGCCATCTG CACAGACAGG 
ATCTGGGGAC TTGGACGACA AGGATATAAA TGCATCAACT GCAAACTGCT GGTTCATAAG 
AAGTGCCACA AGCTTGTCAC AATTGAGTGT GGGCGGCATT CTTTGCCACC GGAACCCATG 
ATGCCAATGG ACCAGTCATC CATGCACCCA GACCACACAC AGACAGTAAT TCCATATAAT 
CCTTCAAGTC ATGAGAGTTT GGACCAAGTT GGTGAAGAAA AGGAGGCAAT GAACACCAGG 
GAGAGTGGGA AGGCGTCATC AAGCTTAGGT CTCCAGGATT TCGATTTGCT TCGAGTTATA 
GGGAGAGGAA GTTACGCCAA AGTACTGCTG GTTCGATTAA AAAAGACAGA TCGCATTTAT 
GCAATGAAGG TTGTGAAGAA AGAGCTCGTC AATGACGATG AGGATATTGA TTGGGTACAG 
ACAGAAAAGC ATGTGTTTGA GCAGGCGTCC AATCACCCTT TCCTTGTTGG TCTGCATTCC 
TGCTTCCAGA CAGAAAGCAG GCTGTTTTTT GTCATAGAAT ATGTGAATGG AGGGGATCTC 
ATGTTTCATA TGCAGCGGCA AAGAAAACTT CCTGAAGAAC ATGCCAGGTT TTACTCAGCA 
GAAATCAGTC TAGCACTAAA TTATCTTCAT GAGCGAGGGA TAATTTATAG AGATTTGAAG 
TTGGACAATG TACTGCTGGA CTCTGAAGGA CACATCAAAC TCACTGACTA CGGCATGTGT 
AAGGAAGGAT TACGGCCCGG AGATACAACC AGCACCTTCT GTGGGACTCC CAATTACATT 
GCTCCTGAGA TCTTAAGAGG AGAAGACTAT GGCTTCAGCG TTGACTGGTG GGCTCTTGGA 
GTACTCATGT TTGAGATGAT GGCGGGAAGG TCTCCATTTG ATATCGTTGG GAGCTCTGAC 
AATCCTGACC AAAACACAGA GGATTATCTG TTCCAAGTCA TTTTGGAGAA GCAGATTCGC 
ATACCGCGCT CCCTGTCTGT GAAAGCAGCA AGTGTGCTGA AGAGTTTCCT CAACAAGGAC 
CCAAAGGAAC GATTGGGTTG TCACCCTCAA ACTGGATTTG CTGACATCCA AGGACACCCA 
TTCTTCCGTA ATGTGGATTG GGACATGATG GAGCAGAAGC AAGTGGTTCC GCCCTTTAAA 
CCAAACATTT CTGGAGAATT TGGTTTGGAT AACTTTGACT CCCAGTTTAC CAACGAACCA 
GTCCAGCTCA CTCCAGATGA TGATGACATC GTGAGGAAGA TTGATCAGTC TGAATTTGAA 
GGTTTCGAGT ATATCAACCC TCTCTTGATG TCTGCAGAAG AGTGTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of microtubule-associated motors drives intermediate filament network polarization.
The Journal of cell biology216(6)1689-1703(2017 Jun)
Leduc C,Etienne-Manneville S

Deletion of Protein Kinase C ? in POMC Neurons Predisposes to Diet-Induced Obesity.
Diabetes66(4)920-934(2017 04)
Dorfman MD,Krull JE,Scarlett JM,Guyenet SJ,Sajan MP,Damian V,Nguyen HT,Leitges M,Morton GJ,Farese RV,Schwartz MW,Thaler JP

Distinct Roles of PKC?/? and PKM? in the Initiation and Maintenance of Hippocampal Long-Term Potentiation and Memory.
Cell reports16(7)1954-61(2016 08)
Wang S,Sheng T,Ren S,Tian T,Lu W

PKC? is critical in AMPA receptor phosphorylation and synaptic incorporation during LTP.
The EMBO journal32(10)1365-80(2013 May)
Ren SQ,Yan JZ,Zhang XY,Bu YF,Pan WW,Yao W,Tian T,Lu W

FGF21 suppresses hepatic glucose production through the activation of atypical protein kinase C?/?.
European journal of pharmacology702(1-3)302-8(2013 Feb)
Kong LJ,Feng W,Wright M,Chen Y,Dallas-yang Q,Zhou YP,Berger JP

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Prkci cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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