LOC108179098 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108179098
Entrez Gene ID	108179098
Full Name	si:dkey-121n8.7
General protein information	Preferred Names si:dkey-121n8.7 Names NACHT, LRR and PYD domains-containing protein 12-like NACHT, LRR and PYD domains-containing protein 3-like
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291920.2	XP_009290195.1	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
XM_693684.8	XP_698776.5	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
XM_009291920.3	XP_009290195.1	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
XM_009291922.1	XP_009290197.1	NACHT, LRR and PYD domains-containing protein 3-like isoform X2
XM_021466479.1	XP_021322154.1	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
XM_693684.7	XP_698776.5	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
XM_009291921.2	XP_009290196.1	NACHT, LRR and PYD domains-containing protein 12-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC108179098 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108179098 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa20821	XM_009291920.3 Latest version!	Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa20821	XM_009291921.2 Latest version!	Danio rerio NACHT, LRR and PYD domains-containing protein 12-like (LOC108179098), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa20821	XM_693684.8 Latest version!	Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa20821	XM_021466479.1 Latest version!	Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa45920	XM_009291922.1 Latest version!	Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
ODa20821	XM_009291920.2	Danio rerio NACHT, LRR and PYD domains-containing protein 12-like (LOC108179098), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa20821	XM_693684.7	Danio rerio NACHT, LRR and PYD domains-containing protein 12-like (LOC108179098), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108179098 ( XM_009291920.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290195.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12-like (LOC108179098), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20821
Clone ID Related Accession (Same CDS sequence)	XM_009291920.2 , XM_693684.8 , XM_009291920.3 , XM_021466479.1 , XM_693684.7 , XM_009291921.2
Accession Version	XM_009291920.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290195.1
CDS	159..3506
Translation	MEDTQTTIEKDYSPRFSLVHQKRSEPSCVSIRRDQSVAHPIDFKSEDTQTHLSSVHQERLDPEPSCVSIRSDRSVAHPIDFKSKNTHLSSVHQERSEPEPSCVSIRSDRSVAHPIDFKSEDTQTHLSTFHQKRKEADPSCVSMRSDTFMNQPINIKIGETQNDLKQNLLNTFRSNLLKKFECLYEGTAVQGNPTLLNEIYTELYITESESGEISHEHEVRQIETQSRRSTTEDTAIKCRDIFTPLPGQNKAIRTVLTKGVAGIGKTVSVQKFILDWAEEKENQDVQLIFPLPFREINLMKDKTLSLSDLLQLFFPQTKEMEISSEKYKVLFIFDGLDECRLSLDFQSAVRLCDVSESASVDVLLTNLIVGNLLPSALIWITSRPAAADLVPSECVHRVTEVRGFNDPQKEEYFSKRISDQSLANTIISHLKSSRSLYIMCHIPVFCWISATVLEKMLSEAETAEIPKTLTQMYTHFLILQTNIKHQKDYEEKLTDEDMILKLGKVAFQQLMKGNIIFYEEDLRECGIDVAEASVYSGLCTQIFREEFGLYQGKVFSFVHLSIQEHLAALYVHLSCLNNNRNEFEQISKQSLWSKVRDHFHFNSSKLVSISKLHQRAVNEALQSKNGHLDLFLRFLLGLSVESHQILLQGLMKLKIRSIQNNETVEYIKKKIRKINFPEKFINLFHCLNELGDHSLVEEIQQFLKSGGMNEAKLSSSQWSAVVFVLLTSEKKLEKFDINEFAARNNNTEQLFIFKKLQPVIRQCRSVQLSYCGLTDEGCAALASALRSNPEHLRDLYLSGNKLGDSVSLLSGVLEDPHCKLETLWLSDCGLTSKDSAALASALRSNPEHLRVLDLSWNRLGDSVTLLSAVLEDPHCKLETLLLRDCGLTDEGCAALASALRSNPEHLKHLDLSWSNLGDSVTLLSAVLEDPRCKLEKLGLSNCGLAEEGCAALASALRSNPEHLRGLNLSVNNLGDSVTLLSAVLEDPRCKLEKIKLSYCGLTDEGCAALASALRSNPEHLRDLDLSTNNLGDSVTLLSAILEDPHCKLEKLELKNCGLTDEGCAVLASALRSNPEHLRDLNLSWNNLSNSTKKLLSDLKDDPHNKLETLHTSLSV

Target ORF information:

RefSeq Version	XM_009291920.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12-like (LOC108179098), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291920.2

ORF Insert Sequence:

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108179098 ( XM_693684.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_698776.5 Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20821
Clone ID Related Accession (Same CDS sequence)	XM_009291920.2 , XM_693684.8 , XM_009291920.3 , XM_021466479.1 , XM_693684.7 , XM_009291921.2
Accession Version	XM_693684.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_693684.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_698776.5
CDS	187..3534
Translation	MEDTQTTIEKDYSPRFSLVHQKRSEPSCVSIRRDQSVAHPIDFKSEDTQTHLSSVHQERLDPEPSCVSIRSDRSVAHPIDFKSKNTHLSSVHQERSEPEPSCVSIRSDRSVAHPIDFKSEDTQTHLSTFHQKRKEADPSCVSMRSDTFMNQPINIKIGETQNDLKQNLLNTFRSNLLKKFECLYEGTAVQGNPTLLNEIYTELYITESESGEISHEHEVRQIETQSRRSTTEDTAIKCRDIFTPLPGQNKAIRTVLTKGVAGIGKTVSVQKFILDWAEEKENQDVQLIFPLPFREINLMKDKTLSLSDLLQLFFPQTKEMEISSEKYKVLFIFDGLDECRLSLDFQSAVRLCDVSESASVDVLLTNLIVGNLLPSALIWITSRPAAADLVPSECVHRVTEVRGFNDPQKEEYFSKRISDQSLANTIISHLKSSRSLYIMCHIPVFCWISATVLEKMLSEAETAEIPKTLTQMYTHFLILQTNIKHQKDYEEKLTDEDMILKLGKVAFQQLMKGNIIFYEEDLRECGIDVAEASVYSGLCTQIFREEFGLYQGKVFSFVHLSIQEHLAALYVHLSCLNNNRNEFEQISKQSLWSKVRDHFHFNSSKLVSISKLHQRAVNEALQSKNGHLDLFLRFLLGLSVESHQILLQGLMKLKIRSIQNNETVEYIKKKIRKINFPEKFINLFHCLNELGDHSLVEEIQQFLKSGGMNEAKLSSSQWSAVVFVLLTSEKKLEKFDINEFAARNNNTEQLFIFKKLQPVIRQCRSVQLSYCGLTDEGCAALASALRSNPEHLRDLYLSGNKLGDSVSLLSGVLEDPHCKLETLWLSDCGLTSKDSAALASALRSNPEHLRVLDLSWNRLGDSVTLLSAVLEDPHCKLETLLLRDCGLTDEGCAALASALRSNPEHLKHLDLSWSNLGDSVTLLSAVLEDPRCKLEKLGLSNCGLAEEGCAALASALRSNPEHLRGLNLSVNNLGDSVTLLSAVLEDPRCKLEKIKLSYCGLTDEGCAALASALRSNPEHLRDLDLSTNNLGDSVTLLSAILEDPHCKLEKLELKNCGLTDEGCAVLASALRSNPEHLRDLNLSWNNLSNSTKKLLSDLKDDPHNKLETLHTSLSV

Target ORF information:

RefSeq Version	XM_693684.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_693684.8

ORF Insert Sequence:

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108179098 ( XM_009291920.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290195.1 Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X2, mRNA.

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CloneID	ODa20821
Clone ID Related Accession (Same CDS sequence)	XM_009291920.2 , XM_693684.8 , XM_009291920.3 , XM_021466479.1 , XM_693684.7 , XM_009291921.2
Accession Version	XM_009291920.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291920.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290195.1
CDS	159..3506
Translation	MEDTQTTIEKDYSPRFSLVHQKRSEPSCVSIRRDQSVAHPIDFKSEDTQTHLSSVHQERLDPEPSCVSIRSDRSVAHPIDFKSKNTHLSSVHQERSEPEPSCVSIRSDRSVAHPIDFKSEDTQTHLSTFHQKRKEADPSCVSMRSDTFMNQPINIKIGETQNDLKQNLLNTFRSNLLKKFECLYEGTAVQGNPTLLNEIYTELYITESESGEISHEHEVRQIETQSRRSTTEDTAIKCRDIFTPLPGQNKAIRTVLTKGVAGIGKTVSVQKFILDWAEEKENQDVQLIFPLPFREINLMKDKTLSLSDLLQLFFPQTKEMEISSEKYKVLFIFDGLDECRLSLDFQSAVRLCDVSESASVDVLLTNLIVGNLLPSALIWITSRPAAADLVPSECVHRVTEVRGFNDPQKEEYFSKRISDQSLANTIISHLKSSRSLYIMCHIPVFCWISATVLEKMLSEAETAEIPKTLTQMYTHFLILQTNIKHQKDYEEKLTDEDMILKLGKVAFQQLMKGNIIFYEEDLRECGIDVAEASVYSGLCTQIFREEFGLYQGKVFSFVHLSIQEHLAALYVHLSCLNNNRNEFEQISKQSLWSKVRDHFHFNSSKLVSISKLHQRAVNEALQSKNGHLDLFLRFLLGLSVESHQILLQGLMKLKIRSIQNNETVEYIKKKIRKINFPEKFINLFHCLNELGDHSLVEEIQQFLKSGGMNEAKLSSSQWSAVVFVLLTSEKKLEKFDINEFAARNNNTEQLFIFKKLQPVIRQCRSVQLSYCGLTDEGCAALASALRSNPEHLRDLYLSGNKLGDSVSLLSGVLEDPHCKLETLWLSDCGLTSKDSAALASALRSNPEHLRVLDLSWNRLGDSVTLLSAVLEDPHCKLETLLLRDCGLTDEGCAALASALRSNPEHLKHLDLSWSNLGDSVTLLSAVLEDPRCKLEKLGLSNCGLAEEGCAALASALRSNPEHLRGLNLSVNNLGDSVTLLSAVLEDPRCKLEKIKLSYCGLTDEGCAALASALRSNPEHLRDLDLSTNNLGDSVTLLSAILEDPHCKLEKLELKNCGLTDEGCAVLASALRSNPEHLRDLNLSWNNLSNSTKKLLSDLKDDPHNKLETLHTSLSV

Target ORF information:

RefSeq Version	XM_009291920.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291920.3

ORF Insert Sequence:

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108179098 ( XM_021466479.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021322154.1 Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X3, mRNA.

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CloneID	ODa20821
Clone ID Related Accession (Same CDS sequence)	XM_009291920.2 , XM_693684.8 , XM_009291920.3 , XM_021466479.1 , XM_693684.7 , XM_009291921.2
Accession Version	XM_021466479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322154.1
CDS	234..3581
Translation	MEDTQTTIEKDYSPRFSLVHQKRSEPSCVSIRRDQSVAHPIDFKSEDTQTHLSSVHQERLDPEPSCVSIRSDRSVAHPIDFKSKNTHLSSVHQERSEPEPSCVSIRSDRSVAHPIDFKSEDTQTHLSTFHQKRKEADPSCVSMRSDTFMNQPINIKIGETQNDLKQNLLNTFRSNLLKKFECLYEGTAVQGNPTLLNEIYTELYITESESGEISHEHEVRQIETQSRRSTTEDTAIKCRDIFTPLPGQNKAIRTVLTKGVAGIGKTVSVQKFILDWAEEKENQDVQLIFPLPFREINLMKDKTLSLSDLLQLFFPQTKEMEISSEKYKVLFIFDGLDECRLSLDFQSAVRLCDVSESASVDVLLTNLIVGNLLPSALIWITSRPAAADLVPSECVHRVTEVRGFNDPQKEEYFSKRISDQSLANTIISHLKSSRSLYIMCHIPVFCWISATVLEKMLSEAETAEIPKTLTQMYTHFLILQTNIKHQKDYEEKLTDEDMILKLGKVAFQQLMKGNIIFYEEDLRECGIDVAEASVYSGLCTQIFREEFGLYQGKVFSFVHLSIQEHLAALYVHLSCLNNNRNEFEQISKQSLWSKVRDHFHFNSSKLVSISKLHQRAVNEALQSKNGHLDLFLRFLLGLSVESHQILLQGLMKLKIRSIQNNETVEYIKKKIRKINFPEKFINLFHCLNELGDHSLVEEIQQFLKSGGMNEAKLSSSQWSAVVFVLLTSEKKLEKFDINEFAARNNNTEQLFIFKKLQPVIRQCRSVQLSYCGLTDEGCAALASALRSNPEHLRDLYLSGNKLGDSVSLLSGVLEDPHCKLETLWLSDCGLTSKDSAALASALRSNPEHLRVLDLSWNRLGDSVTLLSAVLEDPHCKLETLLLRDCGLTDEGCAALASALRSNPEHLKHLDLSWSNLGDSVTLLSAVLEDPRCKLEKLGLSNCGLAEEGCAALASALRSNPEHLRGLNLSVNNLGDSVTLLSAVLEDPRCKLEKIKLSYCGLTDEGCAALASALRSNPEHLRDLDLSTNNLGDSVTLLSAILEDPHCKLEKLELKNCGLTDEGCAVLASALRSNPEHLRDLNLSWNNLSNSTKKLLSDLKDDPHNKLETLHTSLSV

Target ORF information:

RefSeq Version	XM_021466479.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466479.1

ORF Insert Sequence:

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108179098 ( XM_693684.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_698776.5 Danio rerio NACHT, LRR and PYD domains-containing protein 12-like (LOC108179098), transcript variant X1, mRNA.

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CloneID	ODa20821
Clone ID Related Accession (Same CDS sequence)	XM_009291920.2 , XM_693684.8 , XM_009291920.3 , XM_021466479.1 , XM_693684.7 , XM_009291921.2
Accession Version	XM_693684.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_693684.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_698776.5
CDS	187..3534
Translation	MEDTQTTIEKDYSPRFSLVHQKRSEPSCVSIRRDQSVAHPIDFKSEDTQTHLSSVHQERLDPEPSCVSIRSDRSVAHPIDFKSKNTHLSSVHQERSEPEPSCVSIRSDRSVAHPIDFKSEDTQTHLSTFHQKRKEADPSCVSMRSDTFMNQPINIKIGETQNDLKQNLLNTFRSNLLKKFECLYEGTAVQGNPTLLNEIYTELYITESESGEISHEHEVRQIETQSRRSTTEDTAIKCRDIFTPLPGQNKAIRTVLTKGVAGIGKTVSVQKFILDWAEEKENQDVQLIFPLPFREINLMKDKTLSLSDLLQLFFPQTKEMEISSEKYKVLFIFDGLDECRLSLDFQSAVRLCDVSESASVDVLLTNLIVGNLLPSALIWITSRPAAADLVPSECVHRVTEVRGFNDPQKEEYFSKRISDQSLANTIISHLKSSRSLYIMCHIPVFCWISATVLEKMLSEAETAEIPKTLTQMYTHFLILQTNIKHQKDYEEKLTDEDMILKLGKVAFQQLMKGNIIFYEEDLRECGIDVAEASVYSGLCTQIFREEFGLYQGKVFSFVHLSIQEHLAALYVHLSCLNNNRNEFEQISKQSLWSKVRDHFHFNSSKLVSISKLHQRAVNEALQSKNGHLDLFLRFLLGLSVESHQILLQGLMKLKIRSIQNNETVEYIKKKIRKINFPEKFINLFHCLNELGDHSLVEEIQQFLKSGGMNEAKLSSSQWSAVVFVLLTSEKKLEKFDINEFAARNNNTEQLFIFKKLQPVIRQCRSVQLSYCGLTDEGCAALASALRSNPEHLRDLYLSGNKLGDSVSLLSGVLEDPHCKLETLWLSDCGLTSKDSAALASALRSNPEHLRVLDLSWNRLGDSVTLLSAVLEDPHCKLETLLLRDCGLTDEGCAALASALRSNPEHLKHLDLSWSNLGDSVTLLSAVLEDPRCKLEKLGLSNCGLAEEGCAALASALRSNPEHLRGLNLSVNNLGDSVTLLSAVLEDPRCKLEKIKLSYCGLTDEGCAALASALRSNPEHLRDLDLSTNNLGDSVTLLSAILEDPHCKLEKLELKNCGLTDEGCAVLASALRSNPEHLRDLNLSWNNLSNSTKKLLSDLKDDPHNKLETLHTSLSV

Target ORF information:

RefSeq Version	XM_693684.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12-like (LOC108179098), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_693684.7

ORF Insert Sequence:

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108179098 ( XM_009291921.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290196.1 Danio rerio NACHT, LRR and PYD domains-containing protein 12-like (LOC108179098), transcript variant X3, mRNA.

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CloneID	ODa20821
Clone ID Related Accession (Same CDS sequence)	XM_009291920.2 , XM_693684.8 , XM_009291920.3 , XM_021466479.1 , XM_693684.7 , XM_009291921.2
Accession Version	XM_009291921.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291921.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290196.1
CDS	73..3420
Translation	MEDTQTTIEKDYSPRFSLVHQKRSEPSCVSIRRDQSVAHPIDFKSEDTQTHLSSVHQERLDPEPSCVSIRSDRSVAHPIDFKSKNTHLSSVHQERSEPEPSCVSIRSDRSVAHPIDFKSEDTQTHLSTFHQKRKEADPSCVSMRSDTFMNQPINIKIGETQNDLKQNLLNTFRSNLLKKFECLYEGTAVQGNPTLLNEIYTELYITESESGEISHEHEVRQIETQSRRSTTEDTAIKCRDIFTPLPGQNKAIRTVLTKGVAGIGKTVSVQKFILDWAEEKENQDVQLIFPLPFREINLMKDKTLSLSDLLQLFFPQTKEMEISSEKYKVLFIFDGLDECRLSLDFQSAVRLCDVSESASVDVLLTNLIVGNLLPSALIWITSRPAAADLVPSECVHRVTEVRGFNDPQKEEYFSKRISDQSLANTIISHLKSSRSLYIMCHIPVFCWISATVLEKMLSEAETAEIPKTLTQMYTHFLILQTNIKHQKDYEEKLTDEDMILKLGKVAFQQLMKGNIIFYEEDLRECGIDVAEASVYSGLCTQIFREEFGLYQGKVFSFVHLSIQEHLAALYVHLSCLNNNRNEFEQISKQSLWSKVRDHFHFNSSKLVSISKLHQRAVNEALQSKNGHLDLFLRFLLGLSVESHQILLQGLMKLKIRSIQNNETVEYIKKKIRKINFPEKFINLFHCLNELGDHSLVEEIQQFLKSGGMNEAKLSSSQWSAVVFVLLTSEKKLEKFDINEFAARNNNTEQLFIFKKLQPVIRQCRSVQLSYCGLTDEGCAALASALRSNPEHLRDLYLSGNKLGDSVSLLSGVLEDPHCKLETLWLSDCGLTSKDSAALASALRSNPEHLRVLDLSWNRLGDSVTLLSAVLEDPHCKLETLLLRDCGLTDEGCAALASALRSNPEHLKHLDLSWSNLGDSVTLLSAVLEDPRCKLEKLGLSNCGLAEEGCAALASALRSNPEHLRGLNLSVNNLGDSVTLLSAVLEDPRCKLEKIKLSYCGLTDEGCAALASALRSNPEHLRDLDLSTNNLGDSVTLLSAILEDPHCKLEKLELKNCGLTDEGCAVLASALRSNPEHLRDLNLSWNNLSNSTKKLLSDLKDDPHNKLETLHTSLSV

Target ORF information:

RefSeq Version	XM_009291921.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT, LRR and PYD domains-containing protein 12-like (LOC108179098), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291921.2

ORF Insert Sequence:

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTTAA AGAATTGTGG TCTTACAGAT 
GAAGGTTGTG CTGTTTTGGC TTCAGCTCTG AGATCAAACC CTGAACACCT GAGAGATCTG 
AATCTGTCCT GGAATAATCT ATCAAACTCC ACAAAGAAGC TGCTCTCTGA TTTGAAGGAT 
GACCCACATA ATAAACTGGA AACATTACAC ACTTCACTCA GTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108179098 ( XM_009291922.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009290197.1 Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X4, mRNA.

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CloneID	ODa45920
Clone ID Related Accession (Same CDS sequence)	XM_009291922.1
Accession Version	XM_009291922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 3-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290197.1
CDS	187..3345
Translation	MEDTQTTIEKDYSPRFSLVHQKRSEPSCVSIRRDQSVAHPIDFKSEDTQTHLSSVHQERLDPEPSCVSIRSDRSVAHPIDFKSKNTHLSSVHQERSEPEPSCVSIRSDRSVAHPIDFKSEDTQTHLSTFHQKRKEADPSCVSMRSDTFMNQPINIKIGETQNDLKQNLLNTFRSNLLKKFECLYEGTAVQGNPTLLNEIYTELYITESESGEISHEHEVRQIETQSRRSTTEDTAIKCRDIFTPLPGQNKAIRTVLTKGVAGIGKTVSVQKFILDWAEEKENQDVQLIFPLPFREINLMKDKTLSLSDLLQLFFPQTKEMEISSEKYKVLFIFDGLDECRLSLDFQSAVRLCDVSESASVDVLLTNLIVGNLLPSALIWITSRPAAADLVPSECVHRVTEVRGFNDPQKEEYFSKRISDQSLANTIISHLKSSRSLYIMCHIPVFCWISATVLEKMLSEAETAEIPKTLTQMYTHFLILQTNIKHQKDYEEKLTDEDMILKLGKVAFQQLMKGNIIFYEEDLRECGIDVAEASVYSGLCTQIFREEFGLYQGKVFSFVHLSIQEHLAALYVHLSCLNNNRNEFEQISKQSLWSKVRDHFHFNSSKLVSISKLHQRAVNEALQSKNGHLDLFLRFLLGLSVESHQILLQGLMKLKIRSIQNNETVEYIKKKIRKINFPEKFINLFHCLNELGDHSLVEEIQQFLKSGGMNEAKLSSSQWSAVVFVLLTSEKKLEKFDINEFAARNNNTEQLFIFKKLQPVIRQCRSVQLSYCGLTDEGCAALASALRSNPEHLRDLYLSGNKLGDSVSLLSGVLEDPHCKLETLWLSDCGLTSKDSAALASALRSNPEHLRVLDLSWNRLGDSVTLLSAVLEDPHCKLETLLLRDCGLTDEGCAALASALRSNPEHLKHLDLSWSNLGDSVTLLSAVLEDPRCKLEKLGLSNCGLAEEGCAALASALRSNPEHLRGLNLSVNNLGDSVTLLSAVLEDPRCKLEKIKLSYCGLTDEGCAALASALRSNPEHLRDLDLSTNNLGDSVTLLSAILEDPHCKLEKLE

Target ORF information:

RefSeq Version	XM_009291922.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-121n8.7 (si:dkey-121n8.7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291922.1

ORF Insert Sequence:

ATGGAGGACA CACAAACAAC TATAGAGAAG GATTATTCTC CAAGATTCAG TTTAGTCCAT 
CAAAAGAGAT CAGAGCCCAG CTGTGTGTCT ATAAGGAGGG ACCAGTCTGT GGCTCATCCA 
ATAGATTTTA AGAGTGAAGA TACACAGACT CATCTCAGTT CAGTTCACCA GGAGAGATTA 
GATCCAGAGC CCAGCTGTGT GTCTATAAGG AGTGACCGCT CTGTGGCTCA TCCAATAGAT 
TTTAAGAGTA AAAATACTCA TCTCAGTTCA GTTCACCAGG AGAGATCAGA GCCAGAGCCC 
AGCTGTGTGT CTATAAGGAG TGACCGCTCT GTGGCTCATC CAATAGATTT TAAGAGTGAA 
GATACACAGA CTCATCTCAG TACATTTCAT CAGAAGAGGA AAGAAGCAGA TCCCAGCTGT 
GTGTCTATGA GGAGTGATAC GTTTATGAAT CAGCCAATAA ATATAAAGAT TGGAGAAACA 
CAGAATGATC TCAAACAAAA CCTGCTGAAC ACATTTAGAT CAAATCTGCT GAAGAAGTTT 
GAGTGTCTGT ATGAGGGAAC AGCGGTGCAG GGAAACCCAA CACTCCTGAA TGAGATCTAC 
ACAGAGCTCT ACATCACAGA GAGTGAGAGT GGAGAGATCA GTCATGAGCA TGAGGTGAGA 
CAGATTGAGA CACAATCCAG GAGATCAACA ACAGAGGACA CAGCGATCAA ATGCAGAGAC 
ATCTTTACAC CTTTACCTGG ACAGAACAAA GCCATCAGAA CTGTGCTGAC GAAGGGAGTC 
GCTGGCATTG GAAAAACAGT CTCTGTACAG AAGTTCATCC TGGACTGGGC TGAAGAGAAG 
GAGAATCAGG ACGTCCAGCT CATATTTCCA CTTCCTTTCA GAGAGATCAA TCTGATGAAG 
GACAAAACAC TCAGTCTGTC AGATCTTCTT CAGCTCTTTT TCCCTCAAAC TAAAGAAATG 
GAAATATCCA GTGAGAAATA TAAAGTGCTG TTCATCTTTG ATGGTCTGGA CGAGTGTCGT 
CTGTCTCTGG ATTTTCAGAG CGCTGTGAGG TTGTGTGACG TCAGTGAATC CGCCTCAGTG 
GACGTGCTGC TGACCAACCT GATTGTGGGG AATCTGCTTC CCTCTGCTCT CATCTGGATC 
ACCTCCAGAC CAGCAGCAGC AGATCTCGTC CCCTCTGAGT GTGTCCATCG AGTGACAGAG 
GTACGAGGCT TCAATGACCC ACAGAAGGAG GAATACTTCA GCAAGAGAAT CAGTGATCAG 
AGTCTGGCCA ATACAATCAT CTCACACCTG AAGTCCTCCA GGAGCCTCTA CATCATGTGC 
CACATCCCAG TGTTCTGCTG GATCTCAGCC ACTGTTCTGG AGAAGATGTT GAGTGAAGCA 
GAGACTGCAG AGATTCCCAA GACTCTCACT CAGATGTACA CACACTTCCT GATCCTGCAG 
ACCAACATCA AACATCAGAA GGACTATGAG GAGAAGCTGA CAGATGAAGA CATGATCCTC 
AAACTGGGGA AAGTGGCTTT TCAGCAGCTC ATGAAAGGCA ATATAATCTT CTATGAGGAA 
GACCTGAGAG AGTGTGGCAT TGATGTGGCA GAAGCTTCAG TTTACTCAGG ATTGTGCACT 
CAGATCTTCA GAGAGGAGTT TGGCTTGTAT CAGGGGAAAG TCTTCAGCTT TGTTCATCTG 
AGCATTCAGG AACATCTAGC AGCTCTATAT GTGCACCTCT CCTGTTTAAA CAACAACAGA 
AATGAGTTTG AGCAAATCAG CAAACAGAGT TTGTGGTCTA AAGTTAGGGA CCATTTTCAC 
TTTAATTCAT CAAAACTTGT TTCAATATCC AAACTGCATC AGCGAGCTGT AAATGAGGCT 
CTACAGAGTA AAAATGGACA TCTGGATCTT TTCCTGCGGT TTCTTCTGGG TCTGTCAGTG 
GAGTCTCATC AGATTCTTCT ACAAGGACTA ATGAAACTGA AAATCAGATC AATCCAGAAT 
AATGAGACAG TTGAGTACAT CAAGAAGAAG ATCAGGAAAA TTAATTTTCC AGAGAAATTC 
ATCAATCTCT TTCACTGTCT GAATGAACTG GGTGATCATT CACTAGTGGA GGAAATACAG 
CAGTTCCTGA AATCTGGAGG AATGAATGAA GCCAAACTCT CCTCATCTCA GTGGTCAGCT 
GTAGTTTTTG TGTTGTTGAC ATCAGAGAAG AAGCTGGAGA AGTTTGATAT TAATGAATTT 
GCTGCTCGAA ACAATAATAC TGAACAACTG TTCATTTTTA AGAAGCTGCA GCCTGTGATT 
AGACAATGCA GATCAGTTCA GTTGAGTTAT TGTGGTCTCA CAGATGAAGG TTGTGCTGCT 
TTGGCTTCAG CTCTGAGATC AAACCCCGAA CACCTGAGGG ATCTGTATCT GTCTGGGAAT 
AAACTAGGAG ATTCAGTGAG TCTTCTCTCT GGTGTACTGG AGGATCCACA CTGTAAACTG 
GAAACACTGT GGTTGAGTGA TTGTGGTCTT ACAAGTAAAG ATTCTGCTGC TTTGGCTTCA 
GCTCTGAGAT CAAACCCTGA ACACCTGAGA GTGCTGGATC TGTCCTGGAA TAGACTAGGA 
GATTCAGTGA CTCTTCTCTC TGCTGTACTG GAGGATCCTC ACTGTAAACT GGAAACACTG 
TTGTTGAGAG ATTGTGGTCT CACAGATGAA GGTTGTGCTG CTTTGGCTTC AGCTCTGAGA 
TCAAACCCTG AACACCTGAA ACATCTGGAT CTGTCTTGGA GTAATCTGGG AGATTCAGTG 
ACTCTTCTCT CTGCTGTACT GGAGGATCCT CGCTGTAAAC TGGAGAAACT GGGGTTGAGT 
AATTGTGGTC TTGCGGAAGA AGGTTGTGCT GCTTTGGCTT CAGCTCTGAG ATCAAACCCT 
GAACACCTGA GAGGTCTGAA TCTGTCTGTG AATAATCTAG GAGATTCAGT GACTCTTCTC 
TCTGCTGTAC TGGAGGATCC TCGCTGTAAA CTGGAGAAAA TTAAGTTGAG TTATTGTGGT 
CTCACAGATG AAGGTTGTGC TGCTTTGGCC TCAGCTCTGA GATCAAACCC TGAACACCTG 
AGAGATCTGG ATCTGTCTAC GAATAATCTA GGAGATTCAG TGACTCTTCT CTCTGCTATA 
CTGGAGGATC CTCACTGTAA ACTGGAGAAA CTGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC108179098 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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