tet3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tet3
Entrez Gene ID	103283056
Full Name	tet methylcytosine dioxygenase 3
Synonyms	si:ch211-220a11.1
General protein information	Preferred Names tet methylcytosine dioxygenase 3 Names methylcytosine dioxygenase TET3 ten-eleven-translocation 3 tet oncogene family member 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003198607.5	XP_003198655.2	methylcytosine dioxygenase TET3 isoform X1
NM_001327945.1	NP_001314874.1	methylcytosine dioxygenase TET3 isoform 1
XM_009301229.2	XP_009299504.1	tet methylcytosine dioxygenase 3 isoform X1
XM_003198607.4	XP_003198655.2	tet methylcytosine dioxygenase 3 isoform X1
NM_001327946.1	NP_001314875.1	methylcytosine dioxygenase TET3 isoform 2
XM_009301229.3	XP_009299504.1	methylcytosine dioxygenase TET3 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Tet1 facilitates hypoxia tolerance by stabilizing the HIF-? proteins independent of its methylcytosine dioxygenase activity.
Wang J, Zhang D, Du J, Zhou C, Li Z, Liu X, Ouyang G, Xiao W
Nucleic acids research45(22)12700-12714(2017 Dec)

Tet-mediated DNA hydroxymethylation regulates retinal neurogenesis by modulating cell-extrinsic signaling pathways.
Seritrakul P, Gross JM
PLoS genetics13(9)e1006987(2017 Sep)

Zebrafish akt2 is essential for survival, growth, bone development, and glucose homeostasis.
Zhang D, Wang J, Zhou C, Xiao W
Mechanisms of development14342-52(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Through a combination of candidate gene analysis, transcriptomics and pharmacological manipulations, we identified the Notch and Wnt pathways as cell-extrinsic pathways regulated by tet2 and tet3 activity during RGC differentiation and morphogenesis. Transcriptome analyses also revealed the ectopic expression of non-retinal genes in tet2-/-;tet3-/- mutant retinae, and this correlated with locus-specific reduction in 5hmC.
Title: Tet-mediated DNA hydroxymethylation regulates retinal neurogenesis by modulating cell-extrinsic signaling pathways.

these data showed that TET1 and TET3 might be two significant epigenetic regulators involved in Th2 differentiation through regulation of IL-4/13A expression.
Title: TET1 and TET3 are essential in induction of Th2-type immunity partly through regulation of IL-4/13A expression in zebrafish model.

The following tet3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tet3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10183	NM_001327946.1 Latest version!	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa10183	XM_003198607.5 Latest version!	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa10183	XM_009301229.3 Latest version!	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa10182	NM_001327945.1 Latest version!	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
ODa10183	XM_003198607.4	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa10183	XM_009301229.2	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tet3 ( NM_001327946.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001314875.1 Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant 2, mRNA.

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CloneID	ODa10183
Clone ID Related Accession (Same CDS sequence)	XM_003198607.5 , XM_009301229.2 , XM_003198607.4 , NM_001327946.1 , XM_009301229.3
Accession Version	NM_001327946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-12
Organism	Danio rerio(zebrafish)
Product	methylcytosine dioxygenase TET3 isoform 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR388049.7. On May 28, 2016 this sequence version replaced XM_005168617.2. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: GFIL01024896.1, GDQH01006418.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAAATTG GGTCCCATGA ACGCCTGGAA GAAGGCTTAT TAAACCAGGA ACTCTCTAAT 
GGGCTTAGCA AACATGCATC TAATGAAGAA AATGTTTGTG ATAATGAAGA CAGAGATGCA 
ACCAAGGAGC AACACCAGCA TAACTTTCAT GAGGGGGCTG GAAGTATTTC CCAGACACCA 
GGCTGGGTCC TTGAGCATAC TGGGAAGTTG TCAAATCCTG AATCTCAACA AGGTGTTTGG 
AGTGGTCAAA ATGCCAGCAC AACAGTAAAT GATGTGGATA TGGAGGATGC ACAAACTCTG 
GTGGCCTTTT CTGTTGGCTC ACTGCCACCT TACACCAACC ACCAACCTTC ACCGACCCCT 
ACAGCTCAGC TGTATGAAAG ATTCAATCAG GAAATGTCCA ATGGAGGTGA GGAGGCCAAA 
AAGAAAGGTG TTGATGGTGC GTTTTGCCCC CCTGAAGATC TAAACACTCT GCAGGCAGCA 
CTGAGCAAGG CCCGTCATGG CCACAAGCCA CCTAATTGTG ATTGTGATGG CCCAGACTGC 
CCTGATTACC TGGAGTGGCT AGAGAAGCAA ATTAAATTGG TGACTAACTG TCAGAATAAA 
ACTTCCTGTA ATCTTTCGGG TACGGCTCAA CAGCCACTTG AACAGCATCA TTATTCCCAA 
TCCCAACCTC AACAATGTGG AGGTAAACCA TTGTGTGTGC AAGACACAAA TCCACAGCCC 
CTTGTTAACA AGCCTTCTAC TCCTAGACCC TTTGGCCCAC CAGCACCTAT TCCCTGCTCC 
CCTAGTGTTC TGTCCATAGC CAAGGAAAGA AATGTTAGTT TGCAAACAGC CATTGCAATT 
GAGGCTCTGA CCCAGTTGTC TGCCACTGTC CCACAAACTG TTGTACCTCC TGGTGAGTCT 
TCACTTGCAA ATCACCATCA GCATCTGCCA ACCTCCTACC ACCAGAACAT GCCACAAGTT 
GTTTCTTCAT CACCTGGTCC TCTGTTGTCC TCCTCTGCCA CCTCTGCTAT TCCACAGTCA 
AATCATTACC CTCAACAAGA GCTTACAACA TGGGAGCAAC AGAGACCTCA GTCTAAGGGT 
GAGGCCCCTA CAGCAAACAT TTTTCCTAGC TCCTCTCATT ATTCTTCCTC ATACCCATCT 
TCCAAGCCCC CATTTACAAA TATGCACGCA TCAAGCCCCA ATCCTCGACC TCAGCAGTGG 
AACCAGGGCA CAGCTAGTAG CTGTGAGAAA AACTCCCCTC GCAATGCATG GATGATGGCA 
AACACAGACT CTAAACAACA TTTTGCAAAG CCATCCCATG AGAGCAGTGA CCCAGTGTCT 
GAGCTGAAGC AACTTCTTGG AGACACCGGT GGAAAGCACC CGTTCAAGTT CCCATTCCCT 
CACCCCAGCT TACAGGATGT TCATAAGGGC AGCTTTACTG GGATGCCTCA CATAAAGCAG 
GAGGTGGAAA CAAAAGAATA CCTGGGATCA GAATGCTCAG TCATGGGCCA GGAAGGGATG 
CTAAATGGCC AGCAGCAGTT TCAGGGTCAG CAGTTATCCT CCACCATTAG GCACTCAACT 
CAGGCAGCAC TACAGCAGCA CCTTCATCAT AAACGTAATC TCTTCTCTAA TTCCCCAACA 
TATGGTCCAT TAGCCCACAT GGCTTGTCAG GACCTTCGTA AATGGTGGCC TCAGACTATT 
CCAGAGAATC TCATTAACAT CAAACAGGAA CACAAGGAAC CTAAAAAGAA AAAGGGTGCT 
CAAAGTTCTC ATCTAAAGCA GCCAGCTGGT GGGCCTTTTG GTCCACTGGG CACACCTCTT 
CCAAAACCCA AACCGATTGT TATCAAGAAG CATAAACAAA AAGCTTCCCA GCCAACTTTT 
CTACCACAGA TCCAGATTAC AATATCAAAA CCACCTCTCA TTTCTCCAGG TTATGCACCA 
TCCCGAGCTC AGCTTGTTCC ATCTTTGCCA GGCATGGAAG CTGGGCTTCC CCCCACTCAG 
GTGGCTGAGA GCCACCCAGC TCCAGCTCAA TCTCAGGAAT CCATTATAAA TTGCAGTAGT 
GCACCCATCT CTGAAAATAG TCATTCTTCT ACTAACCCTG TGTCAGTCCT AGCACCATCA 
ACCCAGAAGG TAATAGCATG CCCAGGCTCA GCTGGATCAA ATGACAGCAT CCCAACAACC 
AGTATCACAC CTCAAACATC TACAACACAG CCATCTCCTT CTCTGGCTGG CCTCAGTAAC 
CTAGATCCAA AATTTGAAGA GCTGATTCGG CAGTTTGAGG AGGAGTTTGG GGACACTGCA 
GCTTCTGCAG CCGAGACCCC AGAGAATGGC CCTGTTCAAC CAGTGGTGAC CGAGTCGCAC 
CCAAACTCAG GACAATTGAG ACCCGAGTCA CCATCCCTAA AGCAGCCAAA CAACTGCCCT 
CCTGTTCCCA CAAATGAGTC TGAGCCCATG AACGAAGGTC AAAAGGACAT ACCTGAAAGA 
CAGAAGGAAA AAACACCATT GCCACCTGAG GAGCTCTGTA CTGTCAAACA GGAAAGTGAG 
GAATGTGCAT TGGATGTGAA ACCTTCAGGG GAGCTCCTGT CTGAATTTCT GAAGCAACAG 
AACCCCTTCG TTGCACCTTT TTCTCCTCCA AATAAACGTA TGAAGATTGA ATCATCCAAT 
GGTGTGACCG TGCTCTCCAC AACTGCATGT TTCTCTGCAG ACAGAGAGGG TGATGATTTC 
AATACTCCCA CCAAGGATAT CTTTCCCTCC TCACCTTCAC TTAAAGGTTT TCTTGAGTCA 
CCATTGCGCT ACCTGGACAC TCCCACCAAG AACCTGCTTG ATACACCTGG GAAAGATGTT 
CAACCTGATT TTCCAATCTG TGATTGCGTA GACCAAGTCC TTGAAAAGGA TGAAGGTCCA 
TATTACAATC ATTTGGGATC TGGACGAGAT ATACCTTCAG TCAGACAACT GATGGAAGAT 
AGGTATGGGG AGAAGGGGGA AGCCGTTCGC ATTGAAAAGG TGGTTTACAC AGGGCGAGAA 
GGAAAGAGCT CTCAGGGATG TCCTATTGCT AAGTGGGTGT TACGTCGCAG CAGTGAAAAG 
GAGAAAGTGT TGTGCGTTGT GAAGCAACGG CCTGGGCACC ACTGTGCCAA CACTGTCATC 
GTTGTTGTCA TCCTGGCCTG GGAAGGTGTG CCCCGTGCTC TGGGAGATAA ACTGTACAGA 
GAAGTCACAG AAACCATCAC CAAATATGGC AATCCCACCA GCCGTCGCTG TGGACTCAAT 
GAAGATCGAA CCTGTGCATG TCAAGGGAAG GACCCAGAGA CCTGTGGAGC TTCATTCTCT 
TTTGGCTGTT CCTGGAGCAT GTATTTCAAT GGATGCAAGT ATGCTCGAAG CAAGGTCCCT 
CGTAAGTTCA GACTGCAGGG AGAGCACCCC AAAGAGGAGG ACAATCTCAG GGATAACTTC 
CAAGCTTTGG CCACACATGT GGCCCCTCTG TATAAGAAGC TAGCTCCCCA GGCCTACAGC 
AACCAGTGTT TACATGAGGA TGTTGCTTCA GACTGTCGGT TGGGATTGAA GGAAGGACGT 
CCATTTTCAG GAATCACTGC ATGCATGGAT TTCTGTGCCC ACGCTCACAA AGACCAGCAT 
AACCTCCACA ATGGCTGCAC TGTGGTGTGC ACTCTCACGA AAGAGGACAA TCGTACAGTG 
GGCACAATCC CAGAAGATGA GCAGCTGCAC GTGCTTCCGC TCTACAAGCT CGCTACAACC 
GACGAGTTTG GTAGTGAGGA GAACCAGCGT CTCAAAATGC AGACAGGAGC CATCCAGGTG 
CTCGCTAACT TCCGTCGAGA GGTTAGAAAA CTTCCAGAAC CTGCAAAATC ATGCCGCCAG 
CGTCGGTTGG AGGCCAAAAA GGCTGCATCA GAGAAAAAGA ACAGGAAATT GCAGCTAGCA 
GAAACTCCAG AGAAGACCGT CAAGATGGAG ATTCGCCATG CAGAGTCACC TCACATTCAG 
CAAGGCAATA AAGCTATTCC AAAACAGGAG GTGAAACCAA CAATTAAAAA AGAGCCTGTC 
GACCACTTCC AGCCCTTCAA TGGAGCCATA CATGGTTACC AAGCACTAGG GAATGGCAAG 
ACAAACCCTG ATCCCCATAA CATGAACAGT TCGTTCTCCT ACCCTGGGAA CTATGCAAGA 
GGTCGCATCC CTGGCAACAG TCATCCTCCT GCACAGAACC CCATCAATGG CTTTCACCCT 
AACCTCCAAG ATATACGATA TGGTTATTAC AACTACCCTT CCAGTGCACT TTTCTCTCCT 
GAGCTCTTGA GATATGATAA AGGAGCCTGG CCCAAAAACG GAGAACCCTC TGCCCAAGCA 
TTTGAACAGA AGCCGGATGT TCAGTGTCTC CAAGCCAATC TGGCACAGGC CTATCCCACC 
CATCATGGAC AGTCTCAGGC ACAAATGGCT GATGCATCCA TTCAAGGCTA CCCACAACCC 
TCTGATATTT CGCAGTGTCG TACTCCATCT ATGTCTCTTG AGCAGACACA CCGCTCAAAA 
CCCATAGTCA AGCAGGAGCC CATGGATGTA CCATTGTATG AAGCAAGATC AGATGGTCAA 
GCGCAGAGCT GCCCGACCAC TCCTAGTACA ACACCAACAC CTGAAGGATG GCATGGGCAC 
AAACCAAACG GCAGTCTAGT ACCTAAAGGC TGGGATGGAA ACATCAGAGC GGCCTCAAAT 
AATTCACCCT TCACACCAGA TAAGCAGAGG CTCCACCAGC AACACCAACA CGAACACCCT 
CAATATCCAC AGCAGCAACA GTGGAATTCC TATTCCTGCC CAAACACTCC AATGGCATCT 
CCTGCCCCAT CTCCGTCCCC ATCCCTAAAA GCAGGTCTAT CACCTGCACC CTCTCCACAC 
CCGTCCACCC CAAGGCAGTG GGGAAGTCCT GCCCCTAGTC CACAACCTAA AGCATGGGGT 
CCCTATGGTC CCATGGGTTA TGGTGCTGGT GTTATCAGGC AAGGCAACCC TGCTGGTGCT 
TACCCTGATA ACATGCTTTC CCAGGCAGGT GTAAATTGTG GCTCAGCACC CCTTGGACTC 
CAGGAGAAAG CCTGGAAGTC TGGTGGTGGG TCAGCAGCAG GTAGCACGCC ATCCCCAGCT 
CCTGAGGGCC GATTGTTTCC CGATGCACTG CAAAGGTCGG ACGGCCAGGC ATGTTGGGAC 
AGTGGAGCAG AGACACAAAG TGAACGAGAT CCTGAAGAGG ACGAGGTTTG GTCGGACAGT 
GAACACAACT TCTTGGATCC CAACATCGGA GGAGTAGCAG TTGCACCTGC TCATGGTTGT 
GTCTTGATTG AGTGTGCCCG TCGAGAACTC CATGCCACCA CACCGCTTAA AAAGCCTGAC 
CGCACTCACC CAAGCCGCAT ATCCCTGGTC TTCTACCAGC ACAAGAACCT GAACCAGCCC 
TGCCATGGTC TGGCTTTGTG GGAATCCAAG ATGAAAATCC TGGCAGAGCG AGCGCGACAA 
CGGCAACAAG AAGCTGCTCT TTTGGGTCTC TCACAGGAAG AAATCAAGGC CTACGGGAAA 
AAGCGCAAAT GGGCGGCTGG GTCAGCAAGC CCGTCCCCTG GACAAACTAA GGACAAACGG 
GAGGGTGTAG TGACACGGAT GGCCCCAACA CAGCATAGCA CCACCATAGT CACAGTTTCA 
CCTTACCCGT CCACACACCT CACTGGTCCT TACAGTCGAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314875.1
CDS	581..6268
Translation	MEIGSHERLEEGLLNQELSNGLSKHASNEENVCDNEDRDATKEQHQHNFHEGAGSISQTPGWVLEHTGKLSNPESQQGVWSGQNASTTVNDVDMEDAQTLVAFSVGSLPPYTNHQPSPTPTAQLYERFNQEMSNGGEEAKKKGVDGAFCPPEDLNTLQAALSKARHGHKPPNCDCDGPDCPDYLEWLEKQIKLVTNCQNKTSCNLSGTAQQPLEQHHYSQSQPQQCGGKPLCVQDTNPQPLVNKPSTPRPFGPPAPIPCSPSVLSIAKERNVSLQTAIAIEALTQLSATVPQTVVPPGESSLANHHQHLPTSYHQNMPQVVSSSPGPLLSSSATSAIPQSNHYPQQELTTWEQQRPQSKGEAPTANIFPSSSHYSSSYPSSKPPFTNMHASSPNPRPQQWNQGTASSCEKNSPRNAWMMANTDSKQHFAKPSHESSDPVSELKQLLGDTGGKHPFKFPFPHPSLQDVHKGSFTGMPHIKQEVETKEYLGSECSVMGQEGMLNGQQQFQGQQLSSTIRHSTQAALQQHLHHKRNLFSNSPTYGPLAHMACQDLRKWWPQTIPENLINIKQEHKEPKKKKGAQSSHLKQPAGGPFGPLGTPLPKPKPIVIKKHKQKASQPTFLPQIQITISKPPLISPGYAPSRAQLVPSLPGMEAGLPPTQVAESHPAPAQSQESIINCSSAPISENSHSSTNPVSVLAPSTQKVIACPGSAGSNDSIPTTSITPQTSTTQPSPSLAGLSNLDPKFEELIRQFEEEFGDTAASAAETPENGPVQPVVTESHPNSGQLRPESPSLKQPNNCPPVPTNESEPMNEGQKDIPERQKEKTPLPPEELCTVKQESEECALDVKPSGELLSEFLKQQNPFVAPFSPPNKRMKIESSNGVTVLSTTACFSADREGDDFNTPTKDIFPSSPSLKGFLESPLRYLDTPTKNLLDTPGKDVQPDFPICDCVDQVLEKDEGPYYNHLGSGRDIPSVRQLMEDRYGEKGEAVRIEKVVYTGREGKSSQGCPIAKWVLRRSSEKEKVLCVVKQRPGHHCANTVIVVVILAWEGVPRALGDKLYREVTETITKYGNPTSRRCGLNEDRTCACQGKDPETCGASFSFGCSWSMYFNGCKYARSKVPRKFRLQGEHPKEEDNLRDNFQALATHVAPLYKKLAPQAYSNQCLHEDVASDCRLGLKEGRPFSGITACMDFCAHAHKDQHNLHNGCTVVCTLTKEDNRTVGTIPEDEQLHVLPLYKLATTDEFGSEENQRLKMQTGAIQVLANFRREVRKLPEPAKSCRQRRLEAKKAASEKKNRKLQLAETPEKTVKMEIRHAESPHIQQGNKAIPKQEVKPTIKKEPVDHFQPFNGAIHGYQALGNGKTNPDPHNMNSSFSYPGNYARGRIPGNSHPPAQNPINGFHPNLQDIRYGYYNYPSSALFSPELLRYDKGAWPKNGEPSAQAFEQKPDVQCLQANLAQAYPTHHGQSQAQMADASIQGYPQPSDISQCRTPSMSLEQTHRSKPIVKQEPMDVPLYEARSDGQAQSCPTTPSTTPTPEGWHGHKPNGSLVPKGWDGNIRAASNNSPFTPDKQRLHQQHQHEHPQYPQQQQWNSYSCPNTPMASPAPSPSPSLKAGLSPAPSPHPSTPRQWGSPAPSPQPKAWGPYGPMGYGAGVIRQGNPAGAYPDNMLSQAGVNCGSAPLGLQEKAWKSGGGSAAGSTPSPAPEGRLFPDALQRSDGQACWDSGAETQSERDPEEDEVWSDSEHNFLDPNIGGVAVAPAHGCVLIECARRELHATTPLKKPDRTHPSRISLVFYQHKNLNQPCHGLALWESKMKILAERARQRQQEAALLGLSQEEIKAYGKKRKWAAGSASPSPGQTKDKREGVVTRMAPTQHSTTIVTVSPYPSTHLTGPYSRFV

Target ORF information:

RefSeq Version	NM_001327946.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001327946.1

ORF Insert Sequence:

ATGGAAATTG GGTCCCATGA ACGCCTGGAA GAAGGCTTAT TAAACCAGGA ACTCTCTAAT 
GGGCTTAGCA AACATGCATC TAATGAAGAA AATGTTTGTG ATAATGAAGA CAGAGATGCA 
ACCAAGGAGC AACACCAGCA TAACTTTCAT GAGGGGGCTG GAAGTATTTC CCAGACACCA 
GGCTGGGTCC TTGAGCATAC TGGGAAGTTG TCAAATCCTG AATCTCAACA AGGTGTTTGG 
AGTGGTCAAA ATGCCAGCAC AACAGTAAAT GATGTGGATA TGGAGGATGC ACAAACTCTG 
GTGGCCTTTT CTGTTGGCTC ACTGCCACCT TACACCAACC ACCAACCTTC ACCGACCCCT 
ACAGCTCAGC TGTATGAAAG ATTCAATCAG GAAATGTCCA ATGGAGGTGA GGAGGCCAAA 
AAGAAAGGTG TTGATGGTGC GTTTTGCCCC CCTGAAGATC TAAACACTCT GCAGGCAGCA 
CTGAGCAAGG CCCGTCATGG CCACAAGCCA CCTAATTGTG ATTGTGATGG CCCAGACTGC 
CCTGATTACC TGGAGTGGCT AGAGAAGCAA ATTAAATTGG TGACTAACTG TCAGAATAAA 
ACTTCCTGTA ATCTTTCGGG TACGGCTCAA CAGCCACTTG AACAGCATCA TTATTCCCAA 
TCCCAACCTC AACAATGTGG AGGTAAACCA TTGTGTGTGC AAGACACAAA TCCACAGCCC 
CTTGTTAACA AGCCTTCTAC TCCTAGACCC TTTGGCCCAC CAGCACCTAT TCCCTGCTCC 
CCTAGTGTTC TGTCCATAGC CAAGGAAAGA AATGTTAGTT TGCAAACAGC CATTGCAATT 
GAGGCTCTGA CCCAGTTGTC TGCCACTGTC CCACAAACTG TTGTACCTCC TGGTGAGTCT 
TCACTTGCAA ATCACCATCA GCATCTGCCA ACCTCCTACC ACCAGAACAT GCCACAAGTT 
GTTTCTTCAT CACCTGGTCC TCTGTTGTCC TCCTCTGCCA CCTCTGCTAT TCCACAGTCA 
AATCATTACC CTCAACAAGA GCTTACAACA TGGGAGCAAC AGAGACCTCA GTCTAAGGGT 
GAGGCCCCTA CAGCAAACAT TTTTCCTAGC TCCTCTCATT ATTCTTCCTC ATACCCATCT 
TCCAAGCCCC CATTTACAAA TATGCACGCA TCAAGCCCCA ATCCTCGACC TCAGCAGTGG 
AACCAGGGCA CAGCTAGTAG CTGTGAGAAA AACTCCCCTC GCAATGCATG GATGATGGCA 
AACACAGACT CTAAACAACA TTTTGCAAAG CCATCCCATG AGAGCAGTGA CCCAGTGTCT 
GAGCTGAAGC AACTTCTTGG AGACACCGGT GGAAAGCACC CGTTCAAGTT CCCATTCCCT 
CACCCCAGCT TACAGGATGT TCATAAGGGC AGCTTTACTG GGATGCCTCA CATAAAGCAG 
GAGGTGGAAA CAAAAGAATA CCTGGGATCA GAATGCTCAG TCATGGGCCA GGAAGGGATG 
CTAAATGGCC AGCAGCAGTT TCAGGGTCAG CAGTTATCCT CCACCATTAG GCACTCAACT 
CAGGCAGCAC TACAGCAGCA CCTTCATCAT AAACGTAATC TCTTCTCTAA TTCCCCAACA 
TATGGTCCAT TAGCCCACAT GGCTTGTCAG GACCTTCGTA AATGGTGGCC TCAGACTATT 
CCAGAGAATC TCATTAACAT CAAACAGGAA CACAAGGAAC CTAAAAAGAA AAAGGGTGCT 
CAAAGTTCTC ATCTAAAGCA GCCAGCTGGT GGGCCTTTTG GTCCACTGGG CACACCTCTT 
CCAAAACCCA AACCGATTGT TATCAAGAAG CATAAACAAA AAGCTTCCCA GCCAACTTTT 
CTACCACAGA TCCAGATTAC AATATCAAAA CCACCTCTCA TTTCTCCAGG TTATGCACCA 
TCCCGAGCTC AGCTTGTTCC ATCTTTGCCA GGCATGGAAG CTGGGCTTCC CCCCACTCAG 
GTGGCTGAGA GCCACCCAGC TCCAGCTCAA TCTCAGGAAT CCATTATAAA TTGCAGTAGT 
GCACCCATCT CTGAAAATAG TCATTCTTCT ACTAACCCTG TGTCAGTCCT AGCACCATCA 
ACCCAGAAGG TAATAGCATG CCCAGGCTCA GCTGGATCAA ATGACAGCAT CCCAACAACC 
AGTATCACAC CTCAAACATC TACAACACAG CCATCTCCTT CTCTGGCTGG CCTCAGTAAC 
CTAGATCCAA AATTTGAAGA GCTGATTCGG CAGTTTGAGG AGGAGTTTGG GGACACTGCA 
GCTTCTGCAG CCGAGACCCC AGAGAATGGC CCTGTTCAAC CAGTGGTGAC CGAGTCGCAC 
CCAAACTCAG GACAATTGAG ACCCGAGTCA CCATCCCTAA AGCAGCCAAA CAACTGCCCT 
CCTGTTCCCA CAAATGAGTC TGAGCCCATG AACGAAGGTC AAAAGGACAT ACCTGAAAGA 
CAGAAGGAAA AAACACCATT GCCACCTGAG GAGCTCTGTA CTGTCAAACA GGAAAGTGAG 
GAATGTGCAT TGGATGTGAA ACCTTCAGGG GAGCTCCTGT CTGAATTTCT GAAGCAACAG 
AACCCCTTCG TTGCACCTTT TTCTCCTCCA AATAAACGTA TGAAGATTGA ATCATCCAAT 
GGTGTGACCG TGCTCTCCAC AACTGCATGT TTCTCTGCAG ACAGAGAGGG TGATGATTTC 
AATACTCCCA CCAAGGATAT CTTTCCCTCC TCACCTTCAC TTAAAGGTTT TCTTGAGTCA 
CCATTGCGCT ACCTGGACAC TCCCACCAAG AACCTGCTTG ATACACCTGG GAAAGATGTT 
CAACCTGATT TTCCAATCTG TGATTGCGTA GACCAAGTCC TTGAAAAGGA TGAAGGTCCA 
TATTACAATC ATTTGGGATC TGGACGAGAT ATACCTTCAG TCAGACAACT GATGGAAGAT 
AGGTATGGGG AGAAGGGGGA AGCCGTTCGC ATTGAAAAGG TGGTTTACAC AGGGCGAGAA 
GGAAAGAGCT CTCAGGGATG TCCTATTGCT AAGTGGGTGT TACGTCGCAG CAGTGAAAAG 
GAGAAAGTGT TGTGCGTTGT GAAGCAACGG CCTGGGCACC ACTGTGCCAA CACTGTCATC 
GTTGTTGTCA TCCTGGCCTG GGAAGGTGTG CCCCGTGCTC TGGGAGATAA ACTGTACAGA 
GAAGTCACAG AAACCATCAC CAAATATGGC AATCCCACCA GCCGTCGCTG TGGACTCAAT 
GAAGATCGAA CCTGTGCATG TCAAGGGAAG GACCCAGAGA CCTGTGGAGC TTCATTCTCT 
TTTGGCTGTT CCTGGAGCAT GTATTTCAAT GGATGCAAGT ATGCTCGAAG CAAGGTCCCT 
CGTAAGTTCA GACTGCAGGG AGAGCACCCC AAAGAGGAGG ACAATCTCAG GGATAACTTC 
CAAGCTTTGG CCACACATGT GGCCCCTCTG TATAAGAAGC TAGCTCCCCA GGCCTACAGC 
AACCAGTGTT TACATGAGGA TGTTGCTTCA GACTGTCGGT TGGGATTGAA GGAAGGACGT 
CCATTTTCAG GAATCACTGC ATGCATGGAT TTCTGTGCCC ACGCTCACAA AGACCAGCAT 
AACCTCCACA ATGGCTGCAC TGTGGTGTGC ACTCTCACGA AAGAGGACAA TCGTACAGTG 
GGCACAATCC CAGAAGATGA GCAGCTGCAC GTGCTTCCGC TCTACAAGCT CGCTACAACC 
GACGAGTTTG GTAGTGAGGA GAACCAGCGT CTCAAAATGC AGACAGGAGC CATCCAGGTG 
CTCGCTAACT TCCGTCGAGA GGTTAGAAAA CTTCCAGAAC CTGCAAAATC ATGCCGCCAG 
CGTCGGTTGG AGGCCAAAAA GGCTGCATCA GAGAAAAAGA ACAGGAAATT GCAGCTAGCA 
GAAACTCCAG AGAAGACCGT CAAGATGGAG ATTCGCCATG CAGAGTCACC TCACATTCAG 
CAAGGCAATA AAGCTATTCC AAAACAGGAG GTGAAACCAA CAATTAAAAA AGAGCCTGTC 
GACCACTTCC AGCCCTTCAA TGGAGCCATA CATGGTTACC AAGCACTAGG GAATGGCAAG 
ACAAACCCTG ATCCCCATAA CATGAACAGT TCGTTCTCCT ACCCTGGGAA CTATGCAAGA 
GGTCGCATCC CTGGCAACAG TCATCCTCCT GCACAGAACC CCATCAATGG CTTTCACCCT 
AACCTCCAAG ATATACGATA TGGTTATTAC AACTACCCTT CCAGTGCACT TTTCTCTCCT 
GAGCTCTTGA GATATGATAA AGGAGCCTGG CCCAAAAACG GAGAACCCTC TGCCCAAGCA 
TTTGAACAGA AGCCGGATGT TCAGTGTCTC CAAGCCAATC TGGCACAGGC CTATCCCACC 
CATCATGGAC AGTCTCAGGC ACAAATGGCT GATGCATCCA TTCAAGGCTA CCCACAACCC 
TCTGATATTT CGCAGTGTCG TACTCCATCT ATGTCTCTTG AGCAGACACA CCGCTCAAAA 
CCCATAGTCA AGCAGGAGCC CATGGATGTA CCATTGTATG AAGCAAGATC AGATGGTCAA 
GCGCAGAGCT GCCCGACCAC TCCTAGTACA ACACCAACAC CTGAAGGATG GCATGGGCAC 
AAACCAAACG GCAGTCTAGT ACCTAAAGGC TGGGATGGAA ACATCAGAGC GGCCTCAAAT 
AATTCACCCT TCACACCAGA TAAGCAGAGG CTCCACCAGC AACACCAACA CGAACACCCT 
CAATATCCAC AGCAGCAACA GTGGAATTCC TATTCCTGCC CAAACACTCC AATGGCATCT 
CCTGCCCCAT CTCCGTCCCC ATCCCTAAAA GCAGGTCTAT CACCTGCACC CTCTCCACAC 
CCGTCCACCC CAAGGCAGTG GGGAAGTCCT GCCCCTAGTC CACAACCTAA AGCATGGGGT 
CCCTATGGTC CCATGGGTTA TGGTGCTGGT GTTATCAGGC AAGGCAACCC TGCTGGTGCT 
TACCCTGATA ACATGCTTTC CCAGGCAGGT GTAAATTGTG GCTCAGCACC CCTTGGACTC 
CAGGAGAAAG CCTGGAAGTC TGGTGGTGGG TCAGCAGCAG GTAGCACGCC ATCCCCAGCT 
CCTGAGGGCC GATTGTTTCC CGATGCACTG CAAAGGTCGG ACGGCCAGGC ATGTTGGGAC 
AGTGGAGCAG AGACACAAAG TGAACGAGAT CCTGAAGAGG ACGAGGTTTG GTCGGACAGT 
GAACACAACT TCTTGGATCC CAACATCGGA GGAGTAGCAG TTGCACCTGC TCATGGTTGT 
GTCTTGATTG AGTGTGCCCG TCGAGAACTC CATGCCACCA CACCGCTTAA AAAGCCTGAC 
CGCACTCACC CAAGCCGCAT ATCCCTGGTC TTCTACCAGC ACAAGAACCT GAACCAGCCC 
TGCCATGGTC TGGCTTTGTG GGAATCCAAG ATGAAAATCC TGGCAGAGCG AGCGCGACAA 
CGGCAACAAG AAGCTGCTCT TTTGGGTCTC TCACAGGAAG AAATCAAGGC CTACGGGAAA 
AAGCGCAAAT GGGCGGCTGG GTCAGCAAGC CCGTCCCCTG GACAAACTAA GGACAAACGG 
GAGGGTGTAG TGACACGGAT GGCCCCAACA CAGCATAGCA CCACCATAGT CACAGTTTCA 
CCTTACCCGT CCACACACCT CACTGGTCCT TACAGTCGAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tet3 ( XM_003198607.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198655.2 Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X2, mRNA.

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CloneID	ODa10183
Clone ID Related Accession (Same CDS sequence)	XM_003198607.5 , XM_009301229.2 , XM_003198607.4 , NM_001327946.1 , XM_009301229.3
Accession Version	XM_003198607.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	methylcytosine dioxygenase TET3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003198607.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATTG GGTCCCATGA ACGCCTGGAA GAAGGCTTAT TAAACCAGGA ACTCTCTAAT 
GGGCTTAGCA AACATGCATC TAATGAAGAA AATGTTTGTG ATAATGAAGA CAGAGATGCA 
ACCAAGGAGC AACACCAGCA TAACTTTCAT GAGGGGGCTG GAAGTATTTC CCAGACACCA 
GGCTGGGTCC TTGAGCATAC TGGGAAGTTG TCAAATCCTG AATCTCAACA AGGTGTTTGG 
AGTGGTCAAA ATGCCAGCAC AACAGTAAAT GATGTGGATA TGGAGGATGC ACAAACTCTG 
GTGGCCTTTT CTGTTGGCTC ACTGCCACCT TACACCAACC ACCAACCTTC ACCGACCCCT 
ACAGCTCAGC TGTATGAAAG ATTCAATCAG GAAATGTCCA ATGGAGGTGA GGAGGCCAAA 
AAGAAAGGTG TTGATGGTGC GTTTTGCCCC CCTGAAGATC TAAACACTCT GCAGGCAGCA 
CTGAGCAAGG CCCGTCATGG CCACAAGCCA CCTAATTGTG ATTGTGATGG CCCAGACTGC 
CCTGATTACC TGGAGTGGCT AGAGAAGCAA ATTAAATTGG TGACTAACTG TCAGAATAAA 
ACTTCCTGTA ATCTTTCGGG TACGGCTCAA CAGCCACTTG AACAGCATCA TTATTCCCAA 
TCCCAACCTC AACAATGTGG AGGTAAACCA TTGTGTGTGC AAGACACAAA TCCACAGCCC 
CTTGTTAACA AGCCTTCTAC TCCTAGACCC TTTGGCCCAC CAGCACCTAT TCCCTGCTCC 
CCTAGTGTTC TGTCCATAGC CAAGGAAAGA AATGTTAGTT TGCAAACAGC CATTGCAATT 
GAGGCTCTGA CCCAGTTGTC TGCCACTGTC CCACAAACTG TTGTACCTCC TGGTGAGTCT 
TCACTTGCAA ATCACCATCA GCATCTGCCA ACCTCCTACC ACCAGAACAT GCCACAAGTT 
GTTTCTTCAT CACCTGGTCC TCTGTTGTCC TCCTCTGCCA CCTCTGCTAT TCCACAGTCA 
AATCATTACC CTCAACAAGA GCTTACAACA TGGGAGCAAC AGAGACCTCA GTCTAAGGGT 
GAGGCCCCTA CAGCAAACAT TTTTCCTAGC TCCTCTCATT ATTCTTCCTC ATACCCATCT 
TCCAAGCCCC CATTTACAAA TATGCACGCA TCAAGCCCCA ATCCTCGACC TCAGCAGTGG 
AACCAGGGCA CAGCTAGTAG CTGTGAGAAA AACTCCCCTC GCAATGCATG GATGATGGCA 
AACACAGACT CTAAACAACA TTTTGCAAAG CCATCCCATG AGAGCAGTGA CCCAGTGTCT 
GAGCTGAAGC AACTTCTTGG AGACACCGGT GGAAAGCACC CGTTCAAGTT CCCATTCCCT 
CACCCCAGCT TACAGGATGT TCATAAGGGC AGCTTTACTG GGATGCCTCA CATAAAGCAG 
GAGGTGGAAA CAAAAGAATA CCTGGGATCA GAATGCTCAG TCATGGGCCA GGAAGGGATG 
CTAAATGGCC AGCAGCAGTT TCAGGGTCAG CAGTTATCCT CCACCATTAG GCACTCAACT 
CAGGCAGCAC TACAGCAGCA CCTTCATCAT AAACGTAATC TCTTCTCTAA TTCCCCAACA 
TATGGTCCAT TAGCCCACAT GGCTTGTCAG GACCTTCGTA AATGGTGGCC TCAGACTATT 
CCAGAGAATC TCATTAACAT CAAACAGGAA CACAAGGAAC CTAAAAAGAA AAAGGGTGCT 
CAAAGTTCTC ATCTAAAGCA GCCAGCTGGT GGGCCTTTTG GTCCACTGGG CACACCTCTT 
CCAAAACCCA AACCGATTGT TATCAAGAAG CATAAACAAA AAGCTTCCCA GCCAACTTTT 
CTACCACAGA TCCAGATTAC AATATCAAAA CCACCTCTCA TTTCTCCAGG TTATGCACCA 
TCCCGAGCTC AGCTTGTTCC ATCTTTGCCA GGCATGGAAG CTGGGCTTCC CCCCACTCAG 
GTGGCTGAGA GCCACCCAGC TCCAGCTCAA TCTCAGGAAT CCATTATAAA TTGCAGTAGT 
GCACCCATCT CTGAAAATAG TCATTCTTCT ACTAACCCTG TGTCAGTCCT AGCACCATCA 
ACCCAGAAGG TAATAGCATG CCCAGGCTCA GCTGGATCAA ATGACAGCAT CCCAACAACC 
AGTATCACAC CTCAAACATC TACAACACAG CCATCTCCTT CTCTGGCTGG CCTCAGTAAC 
CTAGATCCAA AATTTGAAGA GCTGATTCGG CAGTTTGAGG AGGAGTTTGG GGACACTGCA 
GCTTCTGCAG CCGAGACCCC AGAGAATGGC CCTGTTCAAC CAGTGGTGAC CGAGTCGCAC 
CCAAACTCAG GACAATTGAG ACCCGAGTCA CCATCCCTAA AGCAGCCAAA CAACTGCCCT 
CCTGTTCCCA CAAATGAGTC TGAGCCCATG AACGAAGGTC AAAAGGACAT ACCTGAAAGA 
CAGAAGGAAA AAACACCATT GCCACCTGAG GAGCTCTGTA CTGTCAAACA GGAAAGTGAG 
GAATGTGCAT TGGATGTGAA ACCTTCAGGG GAGCTCCTGT CTGAATTTCT GAAGCAACAG 
AACCCCTTCG TTGCACCTTT TTCTCCTCCA AATAAACGTA TGAAGATTGA ATCATCCAAT 
GGTGTGACCG TGCTCTCCAC AACTGCATGT TTCTCTGCAG ACAGAGAGGG TGATGATTTC 
AATACTCCCA CCAAGGATAT CTTTCCCTCC TCACCTTCAC TTAAAGGTTT TCTTGAGTCA 
CCATTGCGCT ACCTGGACAC TCCCACCAAG AACCTGCTTG ATACACCTGG GAAAGATGTT 
CAACCTGATT TTCCAATCTG TGATTGCGTA GACCAAGTCC TTGAAAAGGA TGAAGGTCCA 
TATTACAATC ATTTGGGATC TGGACGAGAT ATACCTTCAG TCAGACAACT GATGGAAGAT 
AGGTATGGGG AGAAGGGGGA AGCCGTTCGC ATTGAAAAGG TGGTTTACAC AGGGCGAGAA 
GGAAAGAGCT CTCAGGGATG TCCTATTGCT AAGTGGGTGT TACGTCGCAG CAGTGAAAAG 
GAGAAAGTGT TGTGCGTTGT GAAGCAACGG CCTGGGCACC ACTGTGCCAA CACTGTCATC 
GTTGTTGTCA TCCTGGCCTG GGAAGGTGTG CCCCGTGCTC TGGGAGATAA ACTGTACAGA 
GAAGTCACAG AAACCATCAC CAAATATGGC AATCCCACCA GCCGTCGCTG TGGACTCAAT 
GAAGATCGAA CCTGTGCATG TCAAGGGAAG GACCCAGAGA CCTGTGGAGC TTCATTCTCT 
TTTGGCTGTT CCTGGAGCAT GTATTTCAAT GGATGCAAGT ATGCTCGAAG CAAGGTCCCT 
CGTAAGTTCA GACTGCAGGG AGAGCACCCC AAAGAGGAGG ACAATCTCAG GGATAACTTC 
CAAGCTTTGG CCACACATGT GGCCCCTCTG TATAAGAAGC TAGCTCCCCA GGCCTACAGC 
AACCAGTGTT TACATGAGGA TGTTGCTTCA GACTGTCGGT TGGGATTGAA GGAAGGACGT 
CCATTTTCAG GAATCACTGC ATGCATGGAT TTCTGTGCCC ACGCTCACAA AGACCAGCAT 
AACCTCCACA ATGGCTGCAC TGTGGTGTGC ACTCTCACGA AAGAGGACAA TCGTACAGTG 
GGCACAATCC CAGAAGATGA GCAGCTGCAC GTGCTTCCGC TCTACAAGCT CGCTACAACC 
GACGAGTTTG GTAGTGAGGA GAACCAGCGT CTCAAAATGC AGACAGGAGC CATCCAGGTG 
CTCGCTAACT TCCGTCGAGA GGTTAGAAAA CTTCCAGAAC CTGCAAAATC ATGCCGCCAG 
CGTCGGTTGG AGGCCAAAAA GGCTGCATCA GAGAAAAAGA ACAGGAAATT GCAGCTAGCA 
GAAACTCCAG AGAAGACCGT CAAGATGGAG ATTCGCCATG CAGAGTCACC TCACATTCAG 
CAAGGCAATA AAGCTATTCC AAAACAGGAG GTGAAACCAA CAATTAAAAA AGAGCCTGTC 
GACCACTTCC AGCCCTTCAA TGGAGCCATA CATGGTTACC AAGCACTAGG GAATGGCAAG 
ACAAACCCTG ATCCCCATAA CATGAACAGT TCGTTCTCCT ACCCTGGGAA CTATGCAAGA 
GGTCGCATCC CTGGCAACAG TCATCCTCCT GCACAGAACC CCATCAATGG CTTTCACCCT 
AACCTCCAAG ATATACGATA TGGTTATTAC AACTACCCTT CCAGTGCACT TTTCTCTCCT 
GAGCTCTTGA GATATGATAA AGGAGCCTGG CCCAAAAACG GAGAACCCTC TGCCCAAGCA 
TTTGAACAGA AGCCGGATGT TCAGTGTCTC CAAGCCAATC TGGCACAGGC CTATCCCACC 
CATCATGGAC AGTCTCAGGC ACAAATGGCT GATGCATCCA TTCAAGGCTA CCCACAACCC 
TCTGATATTT CGCAGTGTCG TACTCCATCT ATGTCTCTTG AGCAGACACA CCGCTCAAAA 
CCCATAGTCA AGCAGGAGCC CATGGATGTA CCATTGTATG AAGCAAGATC AGATGGTCAA 
GCGCAGAGCT GCCCGACCAC TCCTAGTACA ACACCAACAC CTGAAGGATG GCATGGGCAC 
AAACCAAACG GCAGTCTAGT ACCTAAAGGC TGGGATGGAA ACATCAGAGC GGCCTCAAAT 
AATTCACCCT TCACACCAGA TAAGCAGAGG CTCCACCAGC AACACCAACA CGAACACCCT 
CAATATCCAC AGCAGCAACA GTGGAATTCC TATTCCTGCC CAAACACTCC AATGGCATCT 
CCTGCCCCAT CTCCGTCCCC ATCCCTAAAA GCAGGTCTAT CACCTGCACC CTCTCCACAC 
CCGTCCACCC CAAGGCAGTG GGGAAGTCCT GCCCCTAGTC CACAACCTAA AGCATGGGGT 
CCCTATGGTC CCATGGGTTA TGGTGCTGGT GTTATCAGGC AAGGCAACCC TGCTGGTGCT 
TACCCTGATA ACATGCTTTC CCAGGCAGGT GTAAATTGTG GCTCAGCACC CCTTGGACTC 
CAGGAGAAAG CCTGGAAGTC TGGTGGTGGG TCAGCAGCAG GTAGCACGCC ATCCCCAGCT 
CCTGAGGGCC GATTGTTTCC CGATGCACTG CAAAGGTCGG ACGGCCAGGC ATGTTGGGAC 
AGTGGAGCAG AGACACAAAG TGAACGAGAT CCTGAAGAGG ACGAGGTTTG GTCGGACAGT 
GAACACAACT TCTTGGATCC CAACATCGGA GGAGTAGCAG TTGCACCTGC TCATGGTTGT 
GTCTTGATTG AGTGTGCCCG TCGAGAACTC CATGCCACCA CACCGCTTAA AAAGCCTGAC 
CGCACTCACC CAAGCCGCAT ATCCCTGGTC TTCTACCAGC ACAAGAACCT GAACCAGCCC 
TGCCATGGTC TGGCTTTGTG GGAATCCAAG ATGAAAATCC TGGCAGAGCG AGCGCGACAA 
CGGCAACAAG AAGCTGCTCT TTTGGGTCTC TCACAGGAAG AAATCAAGGC CTACGGGAAA 
AAGCGCAAAT GGGCGGCTGG GTCAGCAAGC CCGTCCCCTG GACAAACTAA GGACAAACGG 
GAGGGTGTAG TGACACGGAT GGCCCCAACA CAGCATAGCA CCACCATAGT CACAGTTTCA 
CCTTACCCGT CCACACACCT CACTGGTCCT TACAGTCGAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198655.2
CDS	422..6109
Translation	MEIGSHERLEEGLLNQELSNGLSKHASNEENVCDNEDRDATKEQHQHNFHEGAGSISQTPGWVLEHTGKLSNPESQQGVWSGQNASTTVNDVDMEDAQTLVAFSVGSLPPYTNHQPSPTPTAQLYERFNQEMSNGGEEAKKKGVDGAFCPPEDLNTLQAALSKARHGHKPPNCDCDGPDCPDYLEWLEKQIKLVTNCQNKTSCNLSGTAQQPLEQHHYSQSQPQQCGGKPLCVQDTNPQPLVNKPSTPRPFGPPAPIPCSPSVLSIAKERNVSLQTAIAIEALTQLSATVPQTVVPPGESSLANHHQHLPTSYHQNMPQVVSSSPGPLLSSSATSAIPQSNHYPQQELTTWEQQRPQSKGEAPTANIFPSSSHYSSSYPSSKPPFTNMHASSPNPRPQQWNQGTASSCEKNSPRNAWMMANTDSKQHFAKPSHESSDPVSELKQLLGDTGGKHPFKFPFPHPSLQDVHKGSFTGMPHIKQEVETKEYLGSECSVMGQEGMLNGQQQFQGQQLSSTIRHSTQAALQQHLHHKRNLFSNSPTYGPLAHMACQDLRKWWPQTIPENLINIKQEHKEPKKKKGAQSSHLKQPAGGPFGPLGTPLPKPKPIVIKKHKQKASQPTFLPQIQITISKPPLISPGYAPSRAQLVPSLPGMEAGLPPTQVAESHPAPAQSQESIINCSSAPISENSHSSTNPVSVLAPSTQKVIACPGSAGSNDSIPTTSITPQTSTTQPSPSLAGLSNLDPKFEELIRQFEEEFGDTAASAAETPENGPVQPVVTESHPNSGQLRPESPSLKQPNNCPPVPTNESEPMNEGQKDIPERQKEKTPLPPEELCTVKQESEECALDVKPSGELLSEFLKQQNPFVAPFSPPNKRMKIESSNGVTVLSTTACFSADREGDDFNTPTKDIFPSSPSLKGFLESPLRYLDTPTKNLLDTPGKDVQPDFPICDCVDQVLEKDEGPYYNHLGSGRDIPSVRQLMEDRYGEKGEAVRIEKVVYTGREGKSSQGCPIAKWVLRRSSEKEKVLCVVKQRPGHHCANTVIVVVILAWEGVPRALGDKLYREVTETITKYGNPTSRRCGLNEDRTCACQGKDPETCGASFSFGCSWSMYFNGCKYARSKVPRKFRLQGEHPKEEDNLRDNFQALATHVAPLYKKLAPQAYSNQCLHEDVASDCRLGLKEGRPFSGITACMDFCAHAHKDQHNLHNGCTVVCTLTKEDNRTVGTIPEDEQLHVLPLYKLATTDEFGSEENQRLKMQTGAIQVLANFRREVRKLPEPAKSCRQRRLEAKKAASEKKNRKLQLAETPEKTVKMEIRHAESPHIQQGNKAIPKQEVKPTIKKEPVDHFQPFNGAIHGYQALGNGKTNPDPHNMNSSFSYPGNYARGRIPGNSHPPAQNPINGFHPNLQDIRYGYYNYPSSALFSPELLRYDKGAWPKNGEPSAQAFEQKPDVQCLQANLAQAYPTHHGQSQAQMADASIQGYPQPSDISQCRTPSMSLEQTHRSKPIVKQEPMDVPLYEARSDGQAQSCPTTPSTTPTPEGWHGHKPNGSLVPKGWDGNIRAASNNSPFTPDKQRLHQQHQHEHPQYPQQQQWNSYSCPNTPMASPAPSPSPSLKAGLSPAPSPHPSTPRQWGSPAPSPQPKAWGPYGPMGYGAGVIRQGNPAGAYPDNMLSQAGVNCGSAPLGLQEKAWKSGGGSAAGSTPSPAPEGRLFPDALQRSDGQACWDSGAETQSERDPEEDEVWSDSEHNFLDPNIGGVAVAPAHGCVLIECARRELHATTPLKKPDRTHPSRISLVFYQHKNLNQPCHGLALWESKMKILAERARQRQQEAALLGLSQEEIKAYGKKRKWAAGSASPSPGQTKDKREGVVTRMAPTQHSTTIVTVSPYPSTHLTGPYSRFV

Target ORF information:

RefSeq Version	XM_003198607.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198607.5

ORF Insert Sequence:

ATGGAAATTG GGTCCCATGA ACGCCTGGAA GAAGGCTTAT TAAACCAGGA ACTCTCTAAT 
GGGCTTAGCA AACATGCATC TAATGAAGAA AATGTTTGTG ATAATGAAGA CAGAGATGCA 
ACCAAGGAGC AACACCAGCA TAACTTTCAT GAGGGGGCTG GAAGTATTTC CCAGACACCA 
GGCTGGGTCC TTGAGCATAC TGGGAAGTTG TCAAATCCTG AATCTCAACA AGGTGTTTGG 
AGTGGTCAAA ATGCCAGCAC AACAGTAAAT GATGTGGATA TGGAGGATGC ACAAACTCTG 
GTGGCCTTTT CTGTTGGCTC ACTGCCACCT TACACCAACC ACCAACCTTC ACCGACCCCT 
ACAGCTCAGC TGTATGAAAG ATTCAATCAG GAAATGTCCA ATGGAGGTGA GGAGGCCAAA 
AAGAAAGGTG TTGATGGTGC GTTTTGCCCC CCTGAAGATC TAAACACTCT GCAGGCAGCA 
CTGAGCAAGG CCCGTCATGG CCACAAGCCA CCTAATTGTG ATTGTGATGG CCCAGACTGC 
CCTGATTACC TGGAGTGGCT AGAGAAGCAA ATTAAATTGG TGACTAACTG TCAGAATAAA 
ACTTCCTGTA ATCTTTCGGG TACGGCTCAA CAGCCACTTG AACAGCATCA TTATTCCCAA 
TCCCAACCTC AACAATGTGG AGGTAAACCA TTGTGTGTGC AAGACACAAA TCCACAGCCC 
CTTGTTAACA AGCCTTCTAC TCCTAGACCC TTTGGCCCAC CAGCACCTAT TCCCTGCTCC 
CCTAGTGTTC TGTCCATAGC CAAGGAAAGA AATGTTAGTT TGCAAACAGC CATTGCAATT 
GAGGCTCTGA CCCAGTTGTC TGCCACTGTC CCACAAACTG TTGTACCTCC TGGTGAGTCT 
TCACTTGCAA ATCACCATCA GCATCTGCCA ACCTCCTACC ACCAGAACAT GCCACAAGTT 
GTTTCTTCAT CACCTGGTCC TCTGTTGTCC TCCTCTGCCA CCTCTGCTAT TCCACAGTCA 
AATCATTACC CTCAACAAGA GCTTACAACA TGGGAGCAAC AGAGACCTCA GTCTAAGGGT 
GAGGCCCCTA CAGCAAACAT TTTTCCTAGC TCCTCTCATT ATTCTTCCTC ATACCCATCT 
TCCAAGCCCC CATTTACAAA TATGCACGCA TCAAGCCCCA ATCCTCGACC TCAGCAGTGG 
AACCAGGGCA CAGCTAGTAG CTGTGAGAAA AACTCCCCTC GCAATGCATG GATGATGGCA 
AACACAGACT CTAAACAACA TTTTGCAAAG CCATCCCATG AGAGCAGTGA CCCAGTGTCT 
GAGCTGAAGC AACTTCTTGG AGACACCGGT GGAAAGCACC CGTTCAAGTT CCCATTCCCT 
CACCCCAGCT TACAGGATGT TCATAAGGGC AGCTTTACTG GGATGCCTCA CATAAAGCAG 
GAGGTGGAAA CAAAAGAATA CCTGGGATCA GAATGCTCAG TCATGGGCCA GGAAGGGATG 
CTAAATGGCC AGCAGCAGTT TCAGGGTCAG CAGTTATCCT CCACCATTAG GCACTCAACT 
CAGGCAGCAC TACAGCAGCA CCTTCATCAT AAACGTAATC TCTTCTCTAA TTCCCCAACA 
TATGGTCCAT TAGCCCACAT GGCTTGTCAG GACCTTCGTA AATGGTGGCC TCAGACTATT 
CCAGAGAATC TCATTAACAT CAAACAGGAA CACAAGGAAC CTAAAAAGAA AAAGGGTGCT 
CAAAGTTCTC ATCTAAAGCA GCCAGCTGGT GGGCCTTTTG GTCCACTGGG CACACCTCTT 
CCAAAACCCA AACCGATTGT TATCAAGAAG CATAAACAAA AAGCTTCCCA GCCAACTTTT 
CTACCACAGA TCCAGATTAC AATATCAAAA CCACCTCTCA TTTCTCCAGG TTATGCACCA 
TCCCGAGCTC AGCTTGTTCC ATCTTTGCCA GGCATGGAAG CTGGGCTTCC CCCCACTCAG 
GTGGCTGAGA GCCACCCAGC TCCAGCTCAA TCTCAGGAAT CCATTATAAA TTGCAGTAGT 
GCACCCATCT CTGAAAATAG TCATTCTTCT ACTAACCCTG TGTCAGTCCT AGCACCATCA 
ACCCAGAAGG TAATAGCATG CCCAGGCTCA GCTGGATCAA ATGACAGCAT CCCAACAACC 
AGTATCACAC CTCAAACATC TACAACACAG CCATCTCCTT CTCTGGCTGG CCTCAGTAAC 
CTAGATCCAA AATTTGAAGA GCTGATTCGG CAGTTTGAGG AGGAGTTTGG GGACACTGCA 
GCTTCTGCAG CCGAGACCCC AGAGAATGGC CCTGTTCAAC CAGTGGTGAC CGAGTCGCAC 
CCAAACTCAG GACAATTGAG ACCCGAGTCA CCATCCCTAA AGCAGCCAAA CAACTGCCCT 
CCTGTTCCCA CAAATGAGTC TGAGCCCATG AACGAAGGTC AAAAGGACAT ACCTGAAAGA 
CAGAAGGAAA AAACACCATT GCCACCTGAG GAGCTCTGTA CTGTCAAACA GGAAAGTGAG 
GAATGTGCAT TGGATGTGAA ACCTTCAGGG GAGCTCCTGT CTGAATTTCT GAAGCAACAG 
AACCCCTTCG TTGCACCTTT TTCTCCTCCA AATAAACGTA TGAAGATTGA ATCATCCAAT 
GGTGTGACCG TGCTCTCCAC AACTGCATGT TTCTCTGCAG ACAGAGAGGG TGATGATTTC 
AATACTCCCA CCAAGGATAT CTTTCCCTCC TCACCTTCAC TTAAAGGTTT TCTTGAGTCA 
CCATTGCGCT ACCTGGACAC TCCCACCAAG AACCTGCTTG ATACACCTGG GAAAGATGTT 
CAACCTGATT TTCCAATCTG TGATTGCGTA GACCAAGTCC TTGAAAAGGA TGAAGGTCCA 
TATTACAATC ATTTGGGATC TGGACGAGAT ATACCTTCAG TCAGACAACT GATGGAAGAT 
AGGTATGGGG AGAAGGGGGA AGCCGTTCGC ATTGAAAAGG TGGTTTACAC AGGGCGAGAA 
GGAAAGAGCT CTCAGGGATG TCCTATTGCT AAGTGGGTGT TACGTCGCAG CAGTGAAAAG 
GAGAAAGTGT TGTGCGTTGT GAAGCAACGG CCTGGGCACC ACTGTGCCAA CACTGTCATC 
GTTGTTGTCA TCCTGGCCTG GGAAGGTGTG CCCCGTGCTC TGGGAGATAA ACTGTACAGA 
GAAGTCACAG AAACCATCAC CAAATATGGC AATCCCACCA GCCGTCGCTG TGGACTCAAT 
GAAGATCGAA CCTGTGCATG TCAAGGGAAG GACCCAGAGA CCTGTGGAGC TTCATTCTCT 
TTTGGCTGTT CCTGGAGCAT GTATTTCAAT GGATGCAAGT ATGCTCGAAG CAAGGTCCCT 
CGTAAGTTCA GACTGCAGGG AGAGCACCCC AAAGAGGAGG ACAATCTCAG GGATAACTTC 
CAAGCTTTGG CCACACATGT GGCCCCTCTG TATAAGAAGC TAGCTCCCCA GGCCTACAGC 
AACCAGTGTT TACATGAGGA TGTTGCTTCA GACTGTCGGT TGGGATTGAA GGAAGGACGT 
CCATTTTCAG GAATCACTGC ATGCATGGAT TTCTGTGCCC ACGCTCACAA AGACCAGCAT 
AACCTCCACA ATGGCTGCAC TGTGGTGTGC ACTCTCACGA AAGAGGACAA TCGTACAGTG 
GGCACAATCC CAGAAGATGA GCAGCTGCAC GTGCTTCCGC TCTACAAGCT CGCTACAACC 
GACGAGTTTG GTAGTGAGGA GAACCAGCGT CTCAAAATGC AGACAGGAGC CATCCAGGTG 
CTCGCTAACT TCCGTCGAGA GGTTAGAAAA CTTCCAGAAC CTGCAAAATC ATGCCGCCAG 
CGTCGGTTGG AGGCCAAAAA GGCTGCATCA GAGAAAAAGA ACAGGAAATT GCAGCTAGCA 
GAAACTCCAG AGAAGACCGT CAAGATGGAG ATTCGCCATG CAGAGTCACC TCACATTCAG 
CAAGGCAATA AAGCTATTCC AAAACAGGAG GTGAAACCAA CAATTAAAAA AGAGCCTGTC 
GACCACTTCC AGCCCTTCAA TGGAGCCATA CATGGTTACC AAGCACTAGG GAATGGCAAG 
ACAAACCCTG ATCCCCATAA CATGAACAGT TCGTTCTCCT ACCCTGGGAA CTATGCAAGA 
GGTCGCATCC CTGGCAACAG TCATCCTCCT GCACAGAACC CCATCAATGG CTTTCACCCT 
AACCTCCAAG ATATACGATA TGGTTATTAC AACTACCCTT CCAGTGCACT TTTCTCTCCT 
GAGCTCTTGA GATATGATAA AGGAGCCTGG CCCAAAAACG GAGAACCCTC TGCCCAAGCA 
TTTGAACAGA AGCCGGATGT TCAGTGTCTC CAAGCCAATC TGGCACAGGC CTATCCCACC 
CATCATGGAC AGTCTCAGGC ACAAATGGCT GATGCATCCA TTCAAGGCTA CCCACAACCC 
TCTGATATTT CGCAGTGTCG TACTCCATCT ATGTCTCTTG AGCAGACACA CCGCTCAAAA 
CCCATAGTCA AGCAGGAGCC CATGGATGTA CCATTGTATG AAGCAAGATC AGATGGTCAA 
GCGCAGAGCT GCCCGACCAC TCCTAGTACA ACACCAACAC CTGAAGGATG GCATGGGCAC 
AAACCAAACG GCAGTCTAGT ACCTAAAGGC TGGGATGGAA ACATCAGAGC GGCCTCAAAT 
AATTCACCCT TCACACCAGA TAAGCAGAGG CTCCACCAGC AACACCAACA CGAACACCCT 
CAATATCCAC AGCAGCAACA GTGGAATTCC TATTCCTGCC CAAACACTCC AATGGCATCT 
CCTGCCCCAT CTCCGTCCCC ATCCCTAAAA GCAGGTCTAT CACCTGCACC CTCTCCACAC 
CCGTCCACCC CAAGGCAGTG GGGAAGTCCT GCCCCTAGTC CACAACCTAA AGCATGGGGT 
CCCTATGGTC CCATGGGTTA TGGTGCTGGT GTTATCAGGC AAGGCAACCC TGCTGGTGCT 
TACCCTGATA ACATGCTTTC CCAGGCAGGT GTAAATTGTG GCTCAGCACC CCTTGGACTC 
CAGGAGAAAG CCTGGAAGTC TGGTGGTGGG TCAGCAGCAG GTAGCACGCC ATCCCCAGCT 
CCTGAGGGCC GATTGTTTCC CGATGCACTG CAAAGGTCGG ACGGCCAGGC ATGTTGGGAC 
AGTGGAGCAG AGACACAAAG TGAACGAGAT CCTGAAGAGG ACGAGGTTTG GTCGGACAGT 
GAACACAACT TCTTGGATCC CAACATCGGA GGAGTAGCAG TTGCACCTGC TCATGGTTGT 
GTCTTGATTG AGTGTGCCCG TCGAGAACTC CATGCCACCA CACCGCTTAA AAAGCCTGAC 
CGCACTCACC CAAGCCGCAT ATCCCTGGTC TTCTACCAGC ACAAGAACCT GAACCAGCCC 
TGCCATGGTC TGGCTTTGTG GGAATCCAAG ATGAAAATCC TGGCAGAGCG AGCGCGACAA 
CGGCAACAAG AAGCTGCTCT TTTGGGTCTC TCACAGGAAG AAATCAAGGC CTACGGGAAA 
AAGCGCAAAT GGGCGGCTGG GTCAGCAAGC CCGTCCCCTG GACAAACTAA GGACAAACGG 
GAGGGTGTAG TGACACGGAT GGCCCCAACA CAGCATAGCA CCACCATAGT CACAGTTTCA 
CCTTACCCGT CCACACACCT CACTGGTCCT TACAGTCGAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tet3 ( XM_009301229.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299504.1 Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X1, mRNA.

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CloneID	ODa10183
Clone ID Related Accession (Same CDS sequence)	XM_003198607.5 , XM_009301229.2 , XM_003198607.4 , NM_001327946.1 , XM_009301229.3
Accession Version	XM_009301229.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tet methylcytosine dioxygenase 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATTG GGTCCCATGA ACGCCTGGAA GAAGGCTTAT TAAACCAGGA ACTCTCTAAT 
GGGCTTAGCA AACATGCATC TAATGAAGAA AATGTTTGTG ATAATGAAGA CAGAGATGCA 
ACCAAGGAGC AACACCAGCA TAACTTTCAT GAGGGGGCTG GAAGTATTTC CCAGACACCA 
GGCTGGGTCC TTGAGCATAC TGGGAAGTTG TCAAATCCTG AATCTCAACA AGGTGTTTGG 
AGTGGTCAAA ATGCCAGCAC AACAGTAAAT GATGTGGATA TGGAGGATGC ACAAACTCTG 
GTGGCCTTTT CTGTTGGCTC ACTGCCACCT TACACCAACC ACCAACCTTC ACCGACCCCT 
ACAGCTCAGC TGTATGAAAG ATTCAATCAG GAAATGTCCA ATGGAGGTGA GGAGGCCAAA 
AAGAAAGGTG TTGATGGTGC GTTTTGCCCC CCTGAAGATC TAAACACTCT GCAGGCAGCA 
CTGAGCAAGG CCCGTCATGG CCACAAGCCA CCTAATTGTG ATTGTGATGG CCCAGACTGC 
CCTGATTACC TGGAGTGGCT AGAGAAGCAA ATTAAATTGG TGACTAACTG TCAGAATAAA 
ACTTCCTGTA ATCTTTCGGG TACGGCTCAA CAGCCACTTG AACAGCATCA TTATTCCCAA 
TCCCAACCTC AACAATGTGG AGGTAAACCA TTGTGTGTGC AAGACACAAA TCCACAGCCC 
CTTGTTAACA AGCCTTCTAC TCCTAGACCC TTTGGCCCAC CAGCACCTAT TCCCTGCTCC 
CCTAGTGTTC TGTCCATAGC CAAGGAAAGA AATGTTAGTT TGCAAACAGC CATTGCAATT 
GAGGCTCTGA CCCAGTTGTC TGCCACTGTC CCACAAACTG TTGTACCTCC TGGTGAGTCT 
TCACTTGCAA ATCACCATCA GCATCTGCCA ACCTCCTACC ACCAGAACAT GCCACAAGTT 
GTTTCTTCAT CACCTGGTCC TCTGTTGTCC TCCTCTGCCA CCTCTGCTAT TCCACAGTCA 
AATCATTACC CTCAACAAGA GCTTACAACA TGGGAGCAAC AGAGACCTCA GTCTAAGGGT 
GAGGCCCCTA CAGCAAACAT TTTTCCTAGC TCCTCTCATT ATTCTTCCTC ATACCCATCT 
TCCAAGCCCC CATTTACAAA TATGCACGCA TCAAGCCCCA ATCCTCGACC TCAGCAGTGG 
AACCAGGGCA CAGCTAGTAG CTGTGAGAAA AACTCCCCTC GCAATGCATG GATGATGGCA 
AACACAGACT CTAAACAACA TTTTGCAAAG CCATCCCATG AGAGCAGTGA CCCAGTGTCT 
GAGCTGAAGC AACTTCTTGG AGACACCGGT GGAAAGCACC CGTTCAAGTT CCCATTCCCT 
CACCCCAGCT TACAGGATGT TCATAAGGGC AGCTTTACTG GGATGCCTCA CATAAAGCAG 
GAGGTGGAAA CAAAAGAATA CCTGGGATCA GAATGCTCAG TCATGGGCCA GGAAGGGATG 
CTAAATGGCC AGCAGCAGTT TCAGGGTCAG CAGTTATCCT CCACCATTAG GCACTCAACT 
CAGGCAGCAC TACAGCAGCA CCTTCATCAT AAACGTAATC TCTTCTCTAA TTCCCCAACA 
TATGGTCCAT TAGCCCACAT GGCTTGTCAG GACCTTCGTA AATGGTGGCC TCAGACTATT 
CCAGAGAATC TCATTAACAT CAAACAGGAA CACAAGGAAC CTAAAAAGAA AAAGGGTGCT 
CAAAGTTCTC ATCTAAAGCA GCCAGCTGGT GGGCCTTTTG GTCCACTGGG CACACCTCTT 
CCAAAACCCA AACCGATTGT TATCAAGAAG CATAAACAAA AAGCTTCCCA GCCAACTTTT 
CTACCACAGA TCCAGATTAC AATATCAAAA CCACCTCTCA TTTCTCCAGG TTATGCACCA 
TCCCGAGCTC AGCTTGTTCC ATCTTTGCCA GGCATGGAAG CTGGGCTTCC CCCCACTCAG 
GTGGCTGAGA GCCACCCAGC TCCAGCTCAA TCTCAGGAAT CCATTATAAA TTGCAGTAGT 
GCACCCATCT CTGAAAATAG TCATTCTTCT ACTAACCCTG TGTCAGTCCT AGCACCATCA 
ACCCAGAAGG TAATAGCATG CCCAGGCTCA GCTGGATCAA ATGACAGCAT CCCAACAACC 
AGTATCACAC CTCAAACATC TACAACACAG CCATCTCCTT CTCTGGCTGG CCTCAGTAAC 
CTAGATCCAA AATTTGAAGA GCTGATTCGG CAGTTTGAGG AGGAGTTTGG GGACACTGCA 
GCTTCTGCAG CCGAGACCCC AGAGAATGGC CCTGTTCAAC CAGTGGTGAC CGAGTCGCAC 
CCAAACTCAG GACAATTGAG ACCCGAGTCA CCATCCCTAA AGCAGCCAAA CAACTGCCCT 
CCTGTTCCCA CAAATGAGTC TGAGCCCATG AACGAAGGTC AAAAGGACAT ACCTGAAAGA 
CAGAAGGAAA AAACACCATT GCCACCTGAG GAGCTCTGTA CTGTCAAACA GGAAAGTGAG 
GAATGTGCAT TGGATGTGAA ACCTTCAGGG GAGCTCCTGT CTGAATTTCT GAAGCAACAG 
AACCCCTTCG TTGCACCTTT TTCTCCTCCA AATAAACGTA TGAAGATTGA ATCATCCAAT 
GGTGTGACCG TGCTCTCCAC AACTGCATGT TTCTCTGCAG ACAGAGAGGG TGATGATTTC 
AATACTCCCA CCAAGGATAT CTTTCCCTCC TCACCTTCAC TTAAAGGTTT TCTTGAGTCA 
CCATTGCGCT ACCTGGACAC TCCCACCAAG AACCTGCTTG ATACACCTGG GAAAGATGTT 
CAACCTGATT TTCCAATCTG TGATTGCGTA GACCAAGTCC TTGAAAAGGA TGAAGGTCCA 
TATTACAATC ATTTGGGATC TGGACGAGAT ATACCTTCAG TCAGACAACT GATGGAAGAT 
AGGTATGGGG AGAAGGGGGA AGCCGTTCGC ATTGAAAAGG TGGTTTACAC AGGGCGAGAA 
GGAAAGAGCT CTCAGGGATG TCCTATTGCT AAGTGGGTGT TACGTCGCAG CAGTGAAAAG 
GAGAAAGTGT TGTGCGTTGT GAAGCAACGG CCTGGGCACC ACTGTGCCAA CACTGTCATC 
GTTGTTGTCA TCCTGGCCTG GGAAGGTGTG CCCCGTGCTC TGGGAGATAA ACTGTACAGA 
GAAGTCACAG AAACCATCAC CAAATATGGC AATCCCACCA GCCGTCGCTG TGGACTCAAT 
GAAGATCGAA CCTGTGCATG TCAAGGGAAG GACCCAGAGA CCTGTGGAGC TTCATTCTCT 
TTTGGCTGTT CCTGGAGCAT GTATTTCAAT GGATGCAAGT ATGCTCGAAG CAAGGTCCCT 
CGTAAGTTCA GACTGCAGGG AGAGCACCCC AAAGAGGAGG ACAATCTCAG GGATAACTTC 
CAAGCTTTGG CCACACATGT GGCCCCTCTG TATAAGAAGC TAGCTCCCCA GGCCTACAGC 
AACCAGTGTT TACATGAGGA TGTTGCTTCA GACTGTCGGT TGGGATTGAA GGAAGGACGT 
CCATTTTCAG GAATCACTGC ATGCATGGAT TTCTGTGCCC ACGCTCACAA AGACCAGCAT 
AACCTCCACA ATGGCTGCAC TGTGGTGTGC ACTCTCACGA AAGAGGACAA TCGTACAGTG 
GGCACAATCC CAGAAGATGA GCAGCTGCAC GTGCTTCCGC TCTACAAGCT CGCTACAACC 
GACGAGTTTG GTAGTGAGGA GAACCAGCGT CTCAAAATGC AGACAGGAGC CATCCAGGTG 
CTCGCTAACT TCCGTCGAGA GGTTAGAAAA CTTCCAGAAC CTGCAAAATC ATGCCGCCAG 
CGTCGGTTGG AGGCCAAAAA GGCTGCATCA GAGAAAAAGA ACAGGAAATT GCAGCTAGCA 
GAAACTCCAG AGAAGACCGT CAAGATGGAG ATTCGCCATG CAGAGTCACC TCACATTCAG 
CAAGGCAATA AAGCTATTCC AAAACAGGAG GTGAAACCAA CAATTAAAAA AGAGCCTGTC 
GACCACTTCC AGCCCTTCAA TGGAGCCATA CATGGTTACC AAGCACTAGG GAATGGCAAG 
ACAAACCCTG ATCCCCATAA CATGAACAGT TCGTTCTCCT ACCCTGGGAA CTATGCAAGA 
GGTCGCATCC CTGGCAACAG TCATCCTCCT GCACAGAACC CCATCAATGG CTTTCACCCT 
AACCTCCAAG ATATACGATA TGGTTATTAC AACTACCCTT CCAGTGCACT TTTCTCTCCT 
GAGCTCTTGA GATATGATAA AGGAGCCTGG CCCAAAAACG GAGAACCCTC TGCCCAAGCA 
TTTGAACAGA AGCCGGATGT TCAGTGTCTC CAAGCCAATC TGGCACAGGC CTATCCCACC 
CATCATGGAC AGTCTCAGGC ACAAATGGCT GATGCATCCA TTCAAGGCTA CCCACAACCC 
TCTGATATTT CGCAGTGTCG TACTCCATCT ATGTCTCTTG AGCAGACACA CCGCTCAAAA 
CCCATAGTCA AGCAGGAGCC CATGGATGTA CCATTGTATG AAGCAAGATC AGATGGTCAA 
GCGCAGAGCT GCCCGACCAC TCCTAGTACA ACACCAACAC CTGAAGGATG GCATGGGCAC 
AAACCAAACG GCAGTCTAGT ACCTAAAGGC TGGGATGGAA ACATCAGAGC GGCCTCAAAT 
AATTCACCCT TCACACCAGA TAAGCAGAGG CTCCACCAGC AACACCAACA CGAACACCCT 
CAATATCCAC AGCAGCAACA GTGGAATTCC TATTCCTGCC CAAACACTCC AATGGCATCT 
CCTGCCCCAT CTCCGTCCCC ATCCCTAAAA GCAGGTCTAT CACCTGCACC CTCTCCACAC 
CCGTCCACCC CAAGGCAGTG GGGAAGTCCT GCCCCTAGTC CACAACCTAA AGCATGGGGT 
CCCTATGGTC CCATGGGTTA TGGTGCTGGT GTTATCAGGC AAGGCAACCC TGCTGGTGCT 
TACCCTGATA ACATGCTTTC CCAGGCAGGT GTAAATTGTG GCTCAGCACC CCTTGGACTC 
CAGGAGAAAG CCTGGAAGTC TGGTGGTGGG TCAGCAGCAG GTAGCACGCC ATCCCCAGCT 
CCTGAGGGCC GATTGTTTCC CGATGCACTG CAAAGGTCGG ACGGCCAGGC ATGTTGGGAC 
AGTGGAGCAG AGACACAAAG TGAACGAGAT CCTGAAGAGG ACGAGGTTTG GTCGGACAGT 
GAACACAACT TCTTGGATCC CAACATCGGA GGAGTAGCAG TTGCACCTGC TCATGGTTGT 
GTCTTGATTG AGTGTGCCCG TCGAGAACTC CATGCCACCA CACCGCTTAA AAAGCCTGAC 
CGCACTCACC CAAGCCGCAT ATCCCTGGTC TTCTACCAGC ACAAGAACCT GAACCAGCCC 
TGCCATGGTC TGGCTTTGTG GGAATCCAAG ATGAAAATCC TGGCAGAGCG AGCGCGACAA 
CGGCAACAAG AAGCTGCTCT TTTGGGTCTC TCACAGGAAG AAATCAAGGC CTACGGGAAA 
AAGCGCAAAT GGGCGGCTGG GTCAGCAAGC CCGTCCCCTG GACAAACTAA GGACAAACGG 
GAGGGTGTAG TGACACGGAT GGCCCCAACA CAGCATAGCA CCACCATAGT CACAGTTTCA 
CCTTACCCGT CCACACACCT CACTGGTCCT TACAGTCGAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299504.1
CDS	915..6602
Translation	MEIGSHERLEEGLLNQELSNGLSKHASNEENVCDNEDRDATKEQHQHNFHEGAGSISQTPGWVLEHTGKLSNPESQQGVWSGQNASTTVNDVDMEDAQTLVAFSVGSLPPYTNHQPSPTPTAQLYERFNQEMSNGGEEAKKKGVDGAFCPPEDLNTLQAALSKARHGHKPPNCDCDGPDCPDYLEWLEKQIKLVTNCQNKTSCNLSGTAQQPLEQHHYSQSQPQQCGGKPLCVQDTNPQPLVNKPSTPRPFGPPAPIPCSPSVLSIAKERNVSLQTAIAIEALTQLSATVPQTVVPPGESSLANHHQHLPTSYHQNMPQVVSSSPGPLLSSSATSAIPQSNHYPQQELTTWEQQRPQSKGEAPTANIFPSSSHYSSSYPSSKPPFTNMHASSPNPRPQQWNQGTASSCEKNSPRNAWMMANTDSKQHFAKPSHESSDPVSELKQLLGDTGGKHPFKFPFPHPSLQDVHKGSFTGMPHIKQEVETKEYLGSECSVMGQEGMLNGQQQFQGQQLSSTIRHSTQAALQQHLHHKRNLFSNSPTYGPLAHMACQDLRKWWPQTIPENLINIKQEHKEPKKKKGAQSSHLKQPAGGPFGPLGTPLPKPKPIVIKKHKQKASQPTFLPQIQITISKPPLISPGYAPSRAQLVPSLPGMEAGLPPTQVAESHPAPAQSQESIINCSSAPISENSHSSTNPVSVLAPSTQKVIACPGSAGSNDSIPTTSITPQTSTTQPSPSLAGLSNLDPKFEELIRQFEEEFGDTAASAAETPENGPVQPVVTESHPNSGQLRPESPSLKQPNNCPPVPTNESEPMNEGQKDIPERQKEKTPLPPEELCTVKQESEECALDVKPSGELLSEFLKQQNPFVAPFSPPNKRMKIESSNGVTVLSTTACFSADREGDDFNTPTKDIFPSSPSLKGFLESPLRYLDTPTKNLLDTPGKDVQPDFPICDCVDQVLEKDEGPYYNHLGSGRDIPSVRQLMEDRYGEKGEAVRIEKVVYTGREGKSSQGCPIAKWVLRRSSEKEKVLCVVKQRPGHHCANTVIVVVILAWEGVPRALGDKLYREVTETITKYGNPTSRRCGLNEDRTCACQGKDPETCGASFSFGCSWSMYFNGCKYARSKVPRKFRLQGEHPKEEDNLRDNFQALATHVAPLYKKLAPQAYSNQCLHEDVASDCRLGLKEGRPFSGITACMDFCAHAHKDQHNLHNGCTVVCTLTKEDNRTVGTIPEDEQLHVLPLYKLATTDEFGSEENQRLKMQTGAIQVLANFRREVRKLPEPAKSCRQRRLEAKKAASEKKNRKLQLAETPEKTVKMEIRHAESPHIQQGNKAIPKQEVKPTIKKEPVDHFQPFNGAIHGYQALGNGKTNPDPHNMNSSFSYPGNYARGRIPGNSHPPAQNPINGFHPNLQDIRYGYYNYPSSALFSPELLRYDKGAWPKNGEPSAQAFEQKPDVQCLQANLAQAYPTHHGQSQAQMADASIQGYPQPSDISQCRTPSMSLEQTHRSKPIVKQEPMDVPLYEARSDGQAQSCPTTPSTTPTPEGWHGHKPNGSLVPKGWDGNIRAASNNSPFTPDKQRLHQQHQHEHPQYPQQQQWNSYSCPNTPMASPAPSPSPSLKAGLSPAPSPHPSTPRQWGSPAPSPQPKAWGPYGPMGYGAGVIRQGNPAGAYPDNMLSQAGVNCGSAPLGLQEKAWKSGGGSAAGSTPSPAPEGRLFPDALQRSDGQACWDSGAETQSERDPEEDEVWSDSEHNFLDPNIGGVAVAPAHGCVLIECARRELHATTPLKKPDRTHPSRISLVFYQHKNLNQPCHGLALWESKMKILAERARQRQQEAALLGLSQEEIKAYGKKRKWAAGSASPSPGQTKDKREGVVTRMAPTQHSTTIVTVSPYPSTHLTGPYSRFV

Target ORF information:

RefSeq Version	XM_009301229.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301229.2

ORF Insert Sequence:

ATGGAAATTG GGTCCCATGA ACGCCTGGAA GAAGGCTTAT TAAACCAGGA ACTCTCTAAT 
GGGCTTAGCA AACATGCATC TAATGAAGAA AATGTTTGTG ATAATGAAGA CAGAGATGCA 
ACCAAGGAGC AACACCAGCA TAACTTTCAT GAGGGGGCTG GAAGTATTTC CCAGACACCA 
GGCTGGGTCC TTGAGCATAC TGGGAAGTTG TCAAATCCTG AATCTCAACA AGGTGTTTGG 
AGTGGTCAAA ATGCCAGCAC AACAGTAAAT GATGTGGATA TGGAGGATGC ACAAACTCTG 
GTGGCCTTTT CTGTTGGCTC ACTGCCACCT TACACCAACC ACCAACCTTC ACCGACCCCT 
ACAGCTCAGC TGTATGAAAG ATTCAATCAG GAAATGTCCA ATGGAGGTGA GGAGGCCAAA 
AAGAAAGGTG TTGATGGTGC GTTTTGCCCC CCTGAAGATC TAAACACTCT GCAGGCAGCA 
CTGAGCAAGG CCCGTCATGG CCACAAGCCA CCTAATTGTG ATTGTGATGG CCCAGACTGC 
CCTGATTACC TGGAGTGGCT AGAGAAGCAA ATTAAATTGG TGACTAACTG TCAGAATAAA 
ACTTCCTGTA ATCTTTCGGG TACGGCTCAA CAGCCACTTG AACAGCATCA TTATTCCCAA 
TCCCAACCTC AACAATGTGG AGGTAAACCA TTGTGTGTGC AAGACACAAA TCCACAGCCC 
CTTGTTAACA AGCCTTCTAC TCCTAGACCC TTTGGCCCAC CAGCACCTAT TCCCTGCTCC 
CCTAGTGTTC TGTCCATAGC CAAGGAAAGA AATGTTAGTT TGCAAACAGC CATTGCAATT 
GAGGCTCTGA CCCAGTTGTC TGCCACTGTC CCACAAACTG TTGTACCTCC TGGTGAGTCT 
TCACTTGCAA ATCACCATCA GCATCTGCCA ACCTCCTACC ACCAGAACAT GCCACAAGTT 
GTTTCTTCAT CACCTGGTCC TCTGTTGTCC TCCTCTGCCA CCTCTGCTAT TCCACAGTCA 
AATCATTACC CTCAACAAGA GCTTACAACA TGGGAGCAAC AGAGACCTCA GTCTAAGGGT 
GAGGCCCCTA CAGCAAACAT TTTTCCTAGC TCCTCTCATT ATTCTTCCTC ATACCCATCT 
TCCAAGCCCC CATTTACAAA TATGCACGCA TCAAGCCCCA ATCCTCGACC TCAGCAGTGG 
AACCAGGGCA CAGCTAGTAG CTGTGAGAAA AACTCCCCTC GCAATGCATG GATGATGGCA 
AACACAGACT CTAAACAACA TTTTGCAAAG CCATCCCATG AGAGCAGTGA CCCAGTGTCT 
GAGCTGAAGC AACTTCTTGG AGACACCGGT GGAAAGCACC CGTTCAAGTT CCCATTCCCT 
CACCCCAGCT TACAGGATGT TCATAAGGGC AGCTTTACTG GGATGCCTCA CATAAAGCAG 
GAGGTGGAAA CAAAAGAATA CCTGGGATCA GAATGCTCAG TCATGGGCCA GGAAGGGATG 
CTAAATGGCC AGCAGCAGTT TCAGGGTCAG CAGTTATCCT CCACCATTAG GCACTCAACT 
CAGGCAGCAC TACAGCAGCA CCTTCATCAT AAACGTAATC TCTTCTCTAA TTCCCCAACA 
TATGGTCCAT TAGCCCACAT GGCTTGTCAG GACCTTCGTA AATGGTGGCC TCAGACTATT 
CCAGAGAATC TCATTAACAT CAAACAGGAA CACAAGGAAC CTAAAAAGAA AAAGGGTGCT 
CAAAGTTCTC ATCTAAAGCA GCCAGCTGGT GGGCCTTTTG GTCCACTGGG CACACCTCTT 
CCAAAACCCA AACCGATTGT TATCAAGAAG CATAAACAAA AAGCTTCCCA GCCAACTTTT 
CTACCACAGA TCCAGATTAC AATATCAAAA CCACCTCTCA TTTCTCCAGG TTATGCACCA 
TCCCGAGCTC AGCTTGTTCC ATCTTTGCCA GGCATGGAAG CTGGGCTTCC CCCCACTCAG 
GTGGCTGAGA GCCACCCAGC TCCAGCTCAA TCTCAGGAAT CCATTATAAA TTGCAGTAGT 
GCACCCATCT CTGAAAATAG TCATTCTTCT ACTAACCCTG TGTCAGTCCT AGCACCATCA 
ACCCAGAAGG TAATAGCATG CCCAGGCTCA GCTGGATCAA ATGACAGCAT CCCAACAACC 
AGTATCACAC CTCAAACATC TACAACACAG CCATCTCCTT CTCTGGCTGG CCTCAGTAAC 
CTAGATCCAA AATTTGAAGA GCTGATTCGG CAGTTTGAGG AGGAGTTTGG GGACACTGCA 
GCTTCTGCAG CCGAGACCCC AGAGAATGGC CCTGTTCAAC CAGTGGTGAC CGAGTCGCAC 
CCAAACTCAG GACAATTGAG ACCCGAGTCA CCATCCCTAA AGCAGCCAAA CAACTGCCCT 
CCTGTTCCCA CAAATGAGTC TGAGCCCATG AACGAAGGTC AAAAGGACAT ACCTGAAAGA 
CAGAAGGAAA AAACACCATT GCCACCTGAG GAGCTCTGTA CTGTCAAACA GGAAAGTGAG 
GAATGTGCAT TGGATGTGAA ACCTTCAGGG GAGCTCCTGT CTGAATTTCT GAAGCAACAG 
AACCCCTTCG TTGCACCTTT TTCTCCTCCA AATAAACGTA TGAAGATTGA ATCATCCAAT 
GGTGTGACCG TGCTCTCCAC AACTGCATGT TTCTCTGCAG ACAGAGAGGG TGATGATTTC 
AATACTCCCA CCAAGGATAT CTTTCCCTCC TCACCTTCAC TTAAAGGTTT TCTTGAGTCA 
CCATTGCGCT ACCTGGACAC TCCCACCAAG AACCTGCTTG ATACACCTGG GAAAGATGTT 
CAACCTGATT TTCCAATCTG TGATTGCGTA GACCAAGTCC TTGAAAAGGA TGAAGGTCCA 
TATTACAATC ATTTGGGATC TGGACGAGAT ATACCTTCAG TCAGACAACT GATGGAAGAT 
AGGTATGGGG AGAAGGGGGA AGCCGTTCGC ATTGAAAAGG TGGTTTACAC AGGGCGAGAA 
GGAAAGAGCT CTCAGGGATG TCCTATTGCT AAGTGGGTGT TACGTCGCAG CAGTGAAAAG 
GAGAAAGTGT TGTGCGTTGT GAAGCAACGG CCTGGGCACC ACTGTGCCAA CACTGTCATC 
GTTGTTGTCA TCCTGGCCTG GGAAGGTGTG CCCCGTGCTC TGGGAGATAA ACTGTACAGA 
GAAGTCACAG AAACCATCAC CAAATATGGC AATCCCACCA GCCGTCGCTG TGGACTCAAT 
GAAGATCGAA CCTGTGCATG TCAAGGGAAG GACCCAGAGA CCTGTGGAGC TTCATTCTCT 
TTTGGCTGTT CCTGGAGCAT GTATTTCAAT GGATGCAAGT ATGCTCGAAG CAAGGTCCCT 
CGTAAGTTCA GACTGCAGGG AGAGCACCCC AAAGAGGAGG ACAATCTCAG GGATAACTTC 
CAAGCTTTGG CCACACATGT GGCCCCTCTG TATAAGAAGC TAGCTCCCCA GGCCTACAGC 
AACCAGTGTT TACATGAGGA TGTTGCTTCA GACTGTCGGT TGGGATTGAA GGAAGGACGT 
CCATTTTCAG GAATCACTGC ATGCATGGAT TTCTGTGCCC ACGCTCACAA AGACCAGCAT 
AACCTCCACA ATGGCTGCAC TGTGGTGTGC ACTCTCACGA AAGAGGACAA TCGTACAGTG 
GGCACAATCC CAGAAGATGA GCAGCTGCAC GTGCTTCCGC TCTACAAGCT CGCTACAACC 
GACGAGTTTG GTAGTGAGGA GAACCAGCGT CTCAAAATGC AGACAGGAGC CATCCAGGTG 
CTCGCTAACT TCCGTCGAGA GGTTAGAAAA CTTCCAGAAC CTGCAAAATC ATGCCGCCAG 
CGTCGGTTGG AGGCCAAAAA GGCTGCATCA GAGAAAAAGA ACAGGAAATT GCAGCTAGCA 
GAAACTCCAG AGAAGACCGT CAAGATGGAG ATTCGCCATG CAGAGTCACC TCACATTCAG 
CAAGGCAATA AAGCTATTCC AAAACAGGAG GTGAAACCAA CAATTAAAAA AGAGCCTGTC 
GACCACTTCC AGCCCTTCAA TGGAGCCATA CATGGTTACC AAGCACTAGG GAATGGCAAG 
ACAAACCCTG ATCCCCATAA CATGAACAGT TCGTTCTCCT ACCCTGGGAA CTATGCAAGA 
GGTCGCATCC CTGGCAACAG TCATCCTCCT GCACAGAACC CCATCAATGG CTTTCACCCT 
AACCTCCAAG ATATACGATA TGGTTATTAC AACTACCCTT CCAGTGCACT TTTCTCTCCT 
GAGCTCTTGA GATATGATAA AGGAGCCTGG CCCAAAAACG GAGAACCCTC TGCCCAAGCA 
TTTGAACAGA AGCCGGATGT TCAGTGTCTC CAAGCCAATC TGGCACAGGC CTATCCCACC 
CATCATGGAC AGTCTCAGGC ACAAATGGCT GATGCATCCA TTCAAGGCTA CCCACAACCC 
TCTGATATTT CGCAGTGTCG TACTCCATCT ATGTCTCTTG AGCAGACACA CCGCTCAAAA 
CCCATAGTCA AGCAGGAGCC CATGGATGTA CCATTGTATG AAGCAAGATC AGATGGTCAA 
GCGCAGAGCT GCCCGACCAC TCCTAGTACA ACACCAACAC CTGAAGGATG GCATGGGCAC 
AAACCAAACG GCAGTCTAGT ACCTAAAGGC TGGGATGGAA ACATCAGAGC GGCCTCAAAT 
AATTCACCCT TCACACCAGA TAAGCAGAGG CTCCACCAGC AACACCAACA CGAACACCCT 
CAATATCCAC AGCAGCAACA GTGGAATTCC TATTCCTGCC CAAACACTCC AATGGCATCT 
CCTGCCCCAT CTCCGTCCCC ATCCCTAAAA GCAGGTCTAT CACCTGCACC CTCTCCACAC 
CCGTCCACCC CAAGGCAGTG GGGAAGTCCT GCCCCTAGTC CACAACCTAA AGCATGGGGT 
CCCTATGGTC CCATGGGTTA TGGTGCTGGT GTTATCAGGC AAGGCAACCC TGCTGGTGCT 
TACCCTGATA ACATGCTTTC CCAGGCAGGT GTAAATTGTG GCTCAGCACC CCTTGGACTC 
CAGGAGAAAG CCTGGAAGTC TGGTGGTGGG TCAGCAGCAG GTAGCACGCC ATCCCCAGCT 
CCTGAGGGCC GATTGTTTCC CGATGCACTG CAAAGGTCGG ACGGCCAGGC ATGTTGGGAC 
AGTGGAGCAG AGACACAAAG TGAACGAGAT CCTGAAGAGG ACGAGGTTTG GTCGGACAGT 
GAACACAACT TCTTGGATCC CAACATCGGA GGAGTAGCAG TTGCACCTGC TCATGGTTGT 
GTCTTGATTG AGTGTGCCCG TCGAGAACTC CATGCCACCA CACCGCTTAA AAAGCCTGAC 
CGCACTCACC CAAGCCGCAT ATCCCTGGTC TTCTACCAGC ACAAGAACCT GAACCAGCCC 
TGCCATGGTC TGGCTTTGTG GGAATCCAAG ATGAAAATCC TGGCAGAGCG AGCGCGACAA 
CGGCAACAAG AAGCTGCTCT TTTGGGTCTC TCACAGGAAG AAATCAAGGC CTACGGGAAA 
AAGCGCAAAT GGGCGGCTGG GTCAGCAAGC CCGTCCCCTG GACAAACTAA GGACAAACGG 
GAGGGTGTAG TGACACGGAT GGCCCCAACA CAGCATAGCA CCACCATAGT CACAGTTTCA 
CCTTACCCGT CCACACACCT CACTGGTCCT TACAGTCGAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tet3 ( XM_003198607.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198655.2 Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X2, mRNA.

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CloneID	ODa10183
Clone ID Related Accession (Same CDS sequence)	XM_003198607.5 , XM_009301229.2 , XM_003198607.4 , NM_001327946.1 , XM_009301229.3
Accession Version	XM_003198607.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tet methylcytosine dioxygenase 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003198607.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATTG GGTCCCATGA ACGCCTGGAA GAAGGCTTAT TAAACCAGGA ACTCTCTAAT 
GGGCTTAGCA AACATGCATC TAATGAAGAA AATGTTTGTG ATAATGAAGA CAGAGATGCA 
ACCAAGGAGC AACACCAGCA TAACTTTCAT GAGGGGGCTG GAAGTATTTC CCAGACACCA 
GGCTGGGTCC TTGAGCATAC TGGGAAGTTG TCAAATCCTG AATCTCAACA AGGTGTTTGG 
AGTGGTCAAA ATGCCAGCAC AACAGTAAAT GATGTGGATA TGGAGGATGC ACAAACTCTG 
GTGGCCTTTT CTGTTGGCTC ACTGCCACCT TACACCAACC ACCAACCTTC ACCGACCCCT 
ACAGCTCAGC TGTATGAAAG ATTCAATCAG GAAATGTCCA ATGGAGGTGA GGAGGCCAAA 
AAGAAAGGTG TTGATGGTGC GTTTTGCCCC CCTGAAGATC TAAACACTCT GCAGGCAGCA 
CTGAGCAAGG CCCGTCATGG CCACAAGCCA CCTAATTGTG ATTGTGATGG CCCAGACTGC 
CCTGATTACC TGGAGTGGCT AGAGAAGCAA ATTAAATTGG TGACTAACTG TCAGAATAAA 
ACTTCCTGTA ATCTTTCGGG TACGGCTCAA CAGCCACTTG AACAGCATCA TTATTCCCAA 
TCCCAACCTC AACAATGTGG AGGTAAACCA TTGTGTGTGC AAGACACAAA TCCACAGCCC 
CTTGTTAACA AGCCTTCTAC TCCTAGACCC TTTGGCCCAC CAGCACCTAT TCCCTGCTCC 
CCTAGTGTTC TGTCCATAGC CAAGGAAAGA AATGTTAGTT TGCAAACAGC CATTGCAATT 
GAGGCTCTGA CCCAGTTGTC TGCCACTGTC CCACAAACTG TTGTACCTCC TGGTGAGTCT 
TCACTTGCAA ATCACCATCA GCATCTGCCA ACCTCCTACC ACCAGAACAT GCCACAAGTT 
GTTTCTTCAT CACCTGGTCC TCTGTTGTCC TCCTCTGCCA CCTCTGCTAT TCCACAGTCA 
AATCATTACC CTCAACAAGA GCTTACAACA TGGGAGCAAC AGAGACCTCA GTCTAAGGGT 
GAGGCCCCTA CAGCAAACAT TTTTCCTAGC TCCTCTCATT ATTCTTCCTC ATACCCATCT 
TCCAAGCCCC CATTTACAAA TATGCACGCA TCAAGCCCCA ATCCTCGACC TCAGCAGTGG 
AACCAGGGCA CAGCTAGTAG CTGTGAGAAA AACTCCCCTC GCAATGCATG GATGATGGCA 
AACACAGACT CTAAACAACA TTTTGCAAAG CCATCCCATG AGAGCAGTGA CCCAGTGTCT 
GAGCTGAAGC AACTTCTTGG AGACACCGGT GGAAAGCACC CGTTCAAGTT CCCATTCCCT 
CACCCCAGCT TACAGGATGT TCATAAGGGC AGCTTTACTG GGATGCCTCA CATAAAGCAG 
GAGGTGGAAA CAAAAGAATA CCTGGGATCA GAATGCTCAG TCATGGGCCA GGAAGGGATG 
CTAAATGGCC AGCAGCAGTT TCAGGGTCAG CAGTTATCCT CCACCATTAG GCACTCAACT 
CAGGCAGCAC TACAGCAGCA CCTTCATCAT AAACGTAATC TCTTCTCTAA TTCCCCAACA 
TATGGTCCAT TAGCCCACAT GGCTTGTCAG GACCTTCGTA AATGGTGGCC TCAGACTATT 
CCAGAGAATC TCATTAACAT CAAACAGGAA CACAAGGAAC CTAAAAAGAA AAAGGGTGCT 
CAAAGTTCTC ATCTAAAGCA GCCAGCTGGT GGGCCTTTTG GTCCACTGGG CACACCTCTT 
CCAAAACCCA AACCGATTGT TATCAAGAAG CATAAACAAA AAGCTTCCCA GCCAACTTTT 
CTACCACAGA TCCAGATTAC AATATCAAAA CCACCTCTCA TTTCTCCAGG TTATGCACCA 
TCCCGAGCTC AGCTTGTTCC ATCTTTGCCA GGCATGGAAG CTGGGCTTCC CCCCACTCAG 
GTGGCTGAGA GCCACCCAGC TCCAGCTCAA TCTCAGGAAT CCATTATAAA TTGCAGTAGT 
GCACCCATCT CTGAAAATAG TCATTCTTCT ACTAACCCTG TGTCAGTCCT AGCACCATCA 
ACCCAGAAGG TAATAGCATG CCCAGGCTCA GCTGGATCAA ATGACAGCAT CCCAACAACC 
AGTATCACAC CTCAAACATC TACAACACAG CCATCTCCTT CTCTGGCTGG CCTCAGTAAC 
CTAGATCCAA AATTTGAAGA GCTGATTCGG CAGTTTGAGG AGGAGTTTGG GGACACTGCA 
GCTTCTGCAG CCGAGACCCC AGAGAATGGC CCTGTTCAAC CAGTGGTGAC CGAGTCGCAC 
CCAAACTCAG GACAATTGAG ACCCGAGTCA CCATCCCTAA AGCAGCCAAA CAACTGCCCT 
CCTGTTCCCA CAAATGAGTC TGAGCCCATG AACGAAGGTC AAAAGGACAT ACCTGAAAGA 
CAGAAGGAAA AAACACCATT GCCACCTGAG GAGCTCTGTA CTGTCAAACA GGAAAGTGAG 
GAATGTGCAT TGGATGTGAA ACCTTCAGGG GAGCTCCTGT CTGAATTTCT GAAGCAACAG 
AACCCCTTCG TTGCACCTTT TTCTCCTCCA AATAAACGTA TGAAGATTGA ATCATCCAAT 
GGTGTGACCG TGCTCTCCAC AACTGCATGT TTCTCTGCAG ACAGAGAGGG TGATGATTTC 
AATACTCCCA CCAAGGATAT CTTTCCCTCC TCACCTTCAC TTAAAGGTTT TCTTGAGTCA 
CCATTGCGCT ACCTGGACAC TCCCACCAAG AACCTGCTTG ATACACCTGG GAAAGATGTT 
CAACCTGATT TTCCAATCTG TGATTGCGTA GACCAAGTCC TTGAAAAGGA TGAAGGTCCA 
TATTACAATC ATTTGGGATC TGGACGAGAT ATACCTTCAG TCAGACAACT GATGGAAGAT 
AGGTATGGGG AGAAGGGGGA AGCCGTTCGC ATTGAAAAGG TGGTTTACAC AGGGCGAGAA 
GGAAAGAGCT CTCAGGGATG TCCTATTGCT AAGTGGGTGT TACGTCGCAG CAGTGAAAAG 
GAGAAAGTGT TGTGCGTTGT GAAGCAACGG CCTGGGCACC ACTGTGCCAA CACTGTCATC 
GTTGTTGTCA TCCTGGCCTG GGAAGGTGTG CCCCGTGCTC TGGGAGATAA ACTGTACAGA 
GAAGTCACAG AAACCATCAC CAAATATGGC AATCCCACCA GCCGTCGCTG TGGACTCAAT 
GAAGATCGAA CCTGTGCATG TCAAGGGAAG GACCCAGAGA CCTGTGGAGC TTCATTCTCT 
TTTGGCTGTT CCTGGAGCAT GTATTTCAAT GGATGCAAGT ATGCTCGAAG CAAGGTCCCT 
CGTAAGTTCA GACTGCAGGG AGAGCACCCC AAAGAGGAGG ACAATCTCAG GGATAACTTC 
CAAGCTTTGG CCACACATGT GGCCCCTCTG TATAAGAAGC TAGCTCCCCA GGCCTACAGC 
AACCAGTGTT TACATGAGGA TGTTGCTTCA GACTGTCGGT TGGGATTGAA GGAAGGACGT 
CCATTTTCAG GAATCACTGC ATGCATGGAT TTCTGTGCCC ACGCTCACAA AGACCAGCAT 
AACCTCCACA ATGGCTGCAC TGTGGTGTGC ACTCTCACGA AAGAGGACAA TCGTACAGTG 
GGCACAATCC CAGAAGATGA GCAGCTGCAC GTGCTTCCGC TCTACAAGCT CGCTACAACC 
GACGAGTTTG GTAGTGAGGA GAACCAGCGT CTCAAAATGC AGACAGGAGC CATCCAGGTG 
CTCGCTAACT TCCGTCGAGA GGTTAGAAAA CTTCCAGAAC CTGCAAAATC ATGCCGCCAG 
CGTCGGTTGG AGGCCAAAAA GGCTGCATCA GAGAAAAAGA ACAGGAAATT GCAGCTAGCA 
GAAACTCCAG AGAAGACCGT CAAGATGGAG ATTCGCCATG CAGAGTCACC TCACATTCAG 
CAAGGCAATA AAGCTATTCC AAAACAGGAG GTGAAACCAA CAATTAAAAA AGAGCCTGTC 
GACCACTTCC AGCCCTTCAA TGGAGCCATA CATGGTTACC AAGCACTAGG GAATGGCAAG 
ACAAACCCTG ATCCCCATAA CATGAACAGT TCGTTCTCCT ACCCTGGGAA CTATGCAAGA 
GGTCGCATCC CTGGCAACAG TCATCCTCCT GCACAGAACC CCATCAATGG CTTTCACCCT 
AACCTCCAAG ATATACGATA TGGTTATTAC AACTACCCTT CCAGTGCACT TTTCTCTCCT 
GAGCTCTTGA GATATGATAA AGGAGCCTGG CCCAAAAACG GAGAACCCTC TGCCCAAGCA 
TTTGAACAGA AGCCGGATGT TCAGTGTCTC CAAGCCAATC TGGCACAGGC CTATCCCACC 
CATCATGGAC AGTCTCAGGC ACAAATGGCT GATGCATCCA TTCAAGGCTA CCCACAACCC 
TCTGATATTT CGCAGTGTCG TACTCCATCT ATGTCTCTTG AGCAGACACA CCGCTCAAAA 
CCCATAGTCA AGCAGGAGCC CATGGATGTA CCATTGTATG AAGCAAGATC AGATGGTCAA 
GCGCAGAGCT GCCCGACCAC TCCTAGTACA ACACCAACAC CTGAAGGATG GCATGGGCAC 
AAACCAAACG GCAGTCTAGT ACCTAAAGGC TGGGATGGAA ACATCAGAGC GGCCTCAAAT 
AATTCACCCT TCACACCAGA TAAGCAGAGG CTCCACCAGC AACACCAACA CGAACACCCT 
CAATATCCAC AGCAGCAACA GTGGAATTCC TATTCCTGCC CAAACACTCC AATGGCATCT 
CCTGCCCCAT CTCCGTCCCC ATCCCTAAAA GCAGGTCTAT CACCTGCACC CTCTCCACAC 
CCGTCCACCC CAAGGCAGTG GGGAAGTCCT GCCCCTAGTC CACAACCTAA AGCATGGGGT 
CCCTATGGTC CCATGGGTTA TGGTGCTGGT GTTATCAGGC AAGGCAACCC TGCTGGTGCT 
TACCCTGATA ACATGCTTTC CCAGGCAGGT GTAAATTGTG GCTCAGCACC CCTTGGACTC 
CAGGAGAAAG CCTGGAAGTC TGGTGGTGGG TCAGCAGCAG GTAGCACGCC ATCCCCAGCT 
CCTGAGGGCC GATTGTTTCC CGATGCACTG CAAAGGTCGG ACGGCCAGGC ATGTTGGGAC 
AGTGGAGCAG AGACACAAAG TGAACGAGAT CCTGAAGAGG ACGAGGTTTG GTCGGACAGT 
GAACACAACT TCTTGGATCC CAACATCGGA GGAGTAGCAG TTGCACCTGC TCATGGTTGT 
GTCTTGATTG AGTGTGCCCG TCGAGAACTC CATGCCACCA CACCGCTTAA AAAGCCTGAC 
CGCACTCACC CAAGCCGCAT ATCCCTGGTC TTCTACCAGC ACAAGAACCT GAACCAGCCC 
TGCCATGGTC TGGCTTTGTG GGAATCCAAG ATGAAAATCC TGGCAGAGCG AGCGCGACAA 
CGGCAACAAG AAGCTGCTCT TTTGGGTCTC TCACAGGAAG AAATCAAGGC CTACGGGAAA 
AAGCGCAAAT GGGCGGCTGG GTCAGCAAGC CCGTCCCCTG GACAAACTAA GGACAAACGG 
GAGGGTGTAG TGACACGGAT GGCCCCAACA CAGCATAGCA CCACCATAGT CACAGTTTCA 
CCTTACCCGT CCACACACCT CACTGGTCCT TACAGTCGAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198655.2
CDS	1315..7002
Translation	MEIGSHERLEEGLLNQELSNGLSKHASNEENVCDNEDRDATKEQHQHNFHEGAGSISQTPGWVLEHTGKLSNPESQQGVWSGQNASTTVNDVDMEDAQTLVAFSVGSLPPYTNHQPSPTPTAQLYERFNQEMSNGGEEAKKKGVDGAFCPPEDLNTLQAALSKARHGHKPPNCDCDGPDCPDYLEWLEKQIKLVTNCQNKTSCNLSGTAQQPLEQHHYSQSQPQQCGGKPLCVQDTNPQPLVNKPSTPRPFGPPAPIPCSPSVLSIAKERNVSLQTAIAIEALTQLSATVPQTVVPPGESSLANHHQHLPTSYHQNMPQVVSSSPGPLLSSSATSAIPQSNHYPQQELTTWEQQRPQSKGEAPTANIFPSSSHYSSSYPSSKPPFTNMHASSPNPRPQQWNQGTASSCEKNSPRNAWMMANTDSKQHFAKPSHESSDPVSELKQLLGDTGGKHPFKFPFPHPSLQDVHKGSFTGMPHIKQEVETKEYLGSECSVMGQEGMLNGQQQFQGQQLSSTIRHSTQAALQQHLHHKRNLFSNSPTYGPLAHMACQDLRKWWPQTIPENLINIKQEHKEPKKKKGAQSSHLKQPAGGPFGPLGTPLPKPKPIVIKKHKQKASQPTFLPQIQITISKPPLISPGYAPSRAQLVPSLPGMEAGLPPTQVAESHPAPAQSQESIINCSSAPISENSHSSTNPVSVLAPSTQKVIACPGSAGSNDSIPTTSITPQTSTTQPSPSLAGLSNLDPKFEELIRQFEEEFGDTAASAAETPENGPVQPVVTESHPNSGQLRPESPSLKQPNNCPPVPTNESEPMNEGQKDIPERQKEKTPLPPEELCTVKQESEECALDVKPSGELLSEFLKQQNPFVAPFSPPNKRMKIESSNGVTVLSTTACFSADREGDDFNTPTKDIFPSSPSLKGFLESPLRYLDTPTKNLLDTPGKDVQPDFPICDCVDQVLEKDEGPYYNHLGSGRDIPSVRQLMEDRYGEKGEAVRIEKVVYTGREGKSSQGCPIAKWVLRRSSEKEKVLCVVKQRPGHHCANTVIVVVILAWEGVPRALGDKLYREVTETITKYGNPTSRRCGLNEDRTCACQGKDPETCGASFSFGCSWSMYFNGCKYARSKVPRKFRLQGEHPKEEDNLRDNFQALATHVAPLYKKLAPQAYSNQCLHEDVASDCRLGLKEGRPFSGITACMDFCAHAHKDQHNLHNGCTVVCTLTKEDNRTVGTIPEDEQLHVLPLYKLATTDEFGSEENQRLKMQTGAIQVLANFRREVRKLPEPAKSCRQRRLEAKKAASEKKNRKLQLAETPEKTVKMEIRHAESPHIQQGNKAIPKQEVKPTIKKEPVDHFQPFNGAIHGYQALGNGKTNPDPHNMNSSFSYPGNYARGRIPGNSHPPAQNPINGFHPNLQDIRYGYYNYPSSALFSPELLRYDKGAWPKNGEPSAQAFEQKPDVQCLQANLAQAYPTHHGQSQAQMADASIQGYPQPSDISQCRTPSMSLEQTHRSKPIVKQEPMDVPLYEARSDGQAQSCPTTPSTTPTPEGWHGHKPNGSLVPKGWDGNIRAASNNSPFTPDKQRLHQQHQHEHPQYPQQQQWNSYSCPNTPMASPAPSPSPSLKAGLSPAPSPHPSTPRQWGSPAPSPQPKAWGPYGPMGYGAGVIRQGNPAGAYPDNMLSQAGVNCGSAPLGLQEKAWKSGGGSAAGSTPSPAPEGRLFPDALQRSDGQACWDSGAETQSERDPEEDEVWSDSEHNFLDPNIGGVAVAPAHGCVLIECARRELHATTPLKKPDRTHPSRISLVFYQHKNLNQPCHGLALWESKMKILAERARQRQQEAALLGLSQEEIKAYGKKRKWAAGSASPSPGQTKDKREGVVTRMAPTQHSTTIVTVSPYPSTHLTGPYSRFV

Target ORF information:

RefSeq Version	XM_003198607.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198607.4

ORF Insert Sequence:

ATGGAAATTG GGTCCCATGA ACGCCTGGAA GAAGGCTTAT TAAACCAGGA ACTCTCTAAT 
GGGCTTAGCA AACATGCATC TAATGAAGAA AATGTTTGTG ATAATGAAGA CAGAGATGCA 
ACCAAGGAGC AACACCAGCA TAACTTTCAT GAGGGGGCTG GAAGTATTTC CCAGACACCA 
GGCTGGGTCC TTGAGCATAC TGGGAAGTTG TCAAATCCTG AATCTCAACA AGGTGTTTGG 
AGTGGTCAAA ATGCCAGCAC AACAGTAAAT GATGTGGATA TGGAGGATGC ACAAACTCTG 
GTGGCCTTTT CTGTTGGCTC ACTGCCACCT TACACCAACC ACCAACCTTC ACCGACCCCT 
ACAGCTCAGC TGTATGAAAG ATTCAATCAG GAAATGTCCA ATGGAGGTGA GGAGGCCAAA 
AAGAAAGGTG TTGATGGTGC GTTTTGCCCC CCTGAAGATC TAAACACTCT GCAGGCAGCA 
CTGAGCAAGG CCCGTCATGG CCACAAGCCA CCTAATTGTG ATTGTGATGG CCCAGACTGC 
CCTGATTACC TGGAGTGGCT AGAGAAGCAA ATTAAATTGG TGACTAACTG TCAGAATAAA 
ACTTCCTGTA ATCTTTCGGG TACGGCTCAA CAGCCACTTG AACAGCATCA TTATTCCCAA 
TCCCAACCTC AACAATGTGG AGGTAAACCA TTGTGTGTGC AAGACACAAA TCCACAGCCC 
CTTGTTAACA AGCCTTCTAC TCCTAGACCC TTTGGCCCAC CAGCACCTAT TCCCTGCTCC 
CCTAGTGTTC TGTCCATAGC CAAGGAAAGA AATGTTAGTT TGCAAACAGC CATTGCAATT 
GAGGCTCTGA CCCAGTTGTC TGCCACTGTC CCACAAACTG TTGTACCTCC TGGTGAGTCT 
TCACTTGCAA ATCACCATCA GCATCTGCCA ACCTCCTACC ACCAGAACAT GCCACAAGTT 
GTTTCTTCAT CACCTGGTCC TCTGTTGTCC TCCTCTGCCA CCTCTGCTAT TCCACAGTCA 
AATCATTACC CTCAACAAGA GCTTACAACA TGGGAGCAAC AGAGACCTCA GTCTAAGGGT 
GAGGCCCCTA CAGCAAACAT TTTTCCTAGC TCCTCTCATT ATTCTTCCTC ATACCCATCT 
TCCAAGCCCC CATTTACAAA TATGCACGCA TCAAGCCCCA ATCCTCGACC TCAGCAGTGG 
AACCAGGGCA CAGCTAGTAG CTGTGAGAAA AACTCCCCTC GCAATGCATG GATGATGGCA 
AACACAGACT CTAAACAACA TTTTGCAAAG CCATCCCATG AGAGCAGTGA CCCAGTGTCT 
GAGCTGAAGC AACTTCTTGG AGACACCGGT GGAAAGCACC CGTTCAAGTT CCCATTCCCT 
CACCCCAGCT TACAGGATGT TCATAAGGGC AGCTTTACTG GGATGCCTCA CATAAAGCAG 
GAGGTGGAAA CAAAAGAATA CCTGGGATCA GAATGCTCAG TCATGGGCCA GGAAGGGATG 
CTAAATGGCC AGCAGCAGTT TCAGGGTCAG CAGTTATCCT CCACCATTAG GCACTCAACT 
CAGGCAGCAC TACAGCAGCA CCTTCATCAT AAACGTAATC TCTTCTCTAA TTCCCCAACA 
TATGGTCCAT TAGCCCACAT GGCTTGTCAG GACCTTCGTA AATGGTGGCC TCAGACTATT 
CCAGAGAATC TCATTAACAT CAAACAGGAA CACAAGGAAC CTAAAAAGAA AAAGGGTGCT 
CAAAGTTCTC ATCTAAAGCA GCCAGCTGGT GGGCCTTTTG GTCCACTGGG CACACCTCTT 
CCAAAACCCA AACCGATTGT TATCAAGAAG CATAAACAAA AAGCTTCCCA GCCAACTTTT 
CTACCACAGA TCCAGATTAC AATATCAAAA CCACCTCTCA TTTCTCCAGG TTATGCACCA 
TCCCGAGCTC AGCTTGTTCC ATCTTTGCCA GGCATGGAAG CTGGGCTTCC CCCCACTCAG 
GTGGCTGAGA GCCACCCAGC TCCAGCTCAA TCTCAGGAAT CCATTATAAA TTGCAGTAGT 
GCACCCATCT CTGAAAATAG TCATTCTTCT ACTAACCCTG TGTCAGTCCT AGCACCATCA 
ACCCAGAAGG TAATAGCATG CCCAGGCTCA GCTGGATCAA ATGACAGCAT CCCAACAACC 
AGTATCACAC CTCAAACATC TACAACACAG CCATCTCCTT CTCTGGCTGG CCTCAGTAAC 
CTAGATCCAA AATTTGAAGA GCTGATTCGG CAGTTTGAGG AGGAGTTTGG GGACACTGCA 
GCTTCTGCAG CCGAGACCCC AGAGAATGGC CCTGTTCAAC CAGTGGTGAC CGAGTCGCAC 
CCAAACTCAG GACAATTGAG ACCCGAGTCA CCATCCCTAA AGCAGCCAAA CAACTGCCCT 
CCTGTTCCCA CAAATGAGTC TGAGCCCATG AACGAAGGTC AAAAGGACAT ACCTGAAAGA 
CAGAAGGAAA AAACACCATT GCCACCTGAG GAGCTCTGTA CTGTCAAACA GGAAAGTGAG 
GAATGTGCAT TGGATGTGAA ACCTTCAGGG GAGCTCCTGT CTGAATTTCT GAAGCAACAG 
AACCCCTTCG TTGCACCTTT TTCTCCTCCA AATAAACGTA TGAAGATTGA ATCATCCAAT 
GGTGTGACCG TGCTCTCCAC AACTGCATGT TTCTCTGCAG ACAGAGAGGG TGATGATTTC 
AATACTCCCA CCAAGGATAT CTTTCCCTCC TCACCTTCAC TTAAAGGTTT TCTTGAGTCA 
CCATTGCGCT ACCTGGACAC TCCCACCAAG AACCTGCTTG ATACACCTGG GAAAGATGTT 
CAACCTGATT TTCCAATCTG TGATTGCGTA GACCAAGTCC TTGAAAAGGA TGAAGGTCCA 
TATTACAATC ATTTGGGATC TGGACGAGAT ATACCTTCAG TCAGACAACT GATGGAAGAT 
AGGTATGGGG AGAAGGGGGA AGCCGTTCGC ATTGAAAAGG TGGTTTACAC AGGGCGAGAA 
GGAAAGAGCT CTCAGGGATG TCCTATTGCT AAGTGGGTGT TACGTCGCAG CAGTGAAAAG 
GAGAAAGTGT TGTGCGTTGT GAAGCAACGG CCTGGGCACC ACTGTGCCAA CACTGTCATC 
GTTGTTGTCA TCCTGGCCTG GGAAGGTGTG CCCCGTGCTC TGGGAGATAA ACTGTACAGA 
GAAGTCACAG AAACCATCAC CAAATATGGC AATCCCACCA GCCGTCGCTG TGGACTCAAT 
GAAGATCGAA CCTGTGCATG TCAAGGGAAG GACCCAGAGA CCTGTGGAGC TTCATTCTCT 
TTTGGCTGTT CCTGGAGCAT GTATTTCAAT GGATGCAAGT ATGCTCGAAG CAAGGTCCCT 
CGTAAGTTCA GACTGCAGGG AGAGCACCCC AAAGAGGAGG ACAATCTCAG GGATAACTTC 
CAAGCTTTGG CCACACATGT GGCCCCTCTG TATAAGAAGC TAGCTCCCCA GGCCTACAGC 
AACCAGTGTT TACATGAGGA TGTTGCTTCA GACTGTCGGT TGGGATTGAA GGAAGGACGT 
CCATTTTCAG GAATCACTGC ATGCATGGAT TTCTGTGCCC ACGCTCACAA AGACCAGCAT 
AACCTCCACA ATGGCTGCAC TGTGGTGTGC ACTCTCACGA AAGAGGACAA TCGTACAGTG 
GGCACAATCC CAGAAGATGA GCAGCTGCAC GTGCTTCCGC TCTACAAGCT CGCTACAACC 
GACGAGTTTG GTAGTGAGGA GAACCAGCGT CTCAAAATGC AGACAGGAGC CATCCAGGTG 
CTCGCTAACT TCCGTCGAGA GGTTAGAAAA CTTCCAGAAC CTGCAAAATC ATGCCGCCAG 
CGTCGGTTGG AGGCCAAAAA GGCTGCATCA GAGAAAAAGA ACAGGAAATT GCAGCTAGCA 
GAAACTCCAG AGAAGACCGT CAAGATGGAG ATTCGCCATG CAGAGTCACC TCACATTCAG 
CAAGGCAATA AAGCTATTCC AAAACAGGAG GTGAAACCAA CAATTAAAAA AGAGCCTGTC 
GACCACTTCC AGCCCTTCAA TGGAGCCATA CATGGTTACC AAGCACTAGG GAATGGCAAG 
ACAAACCCTG ATCCCCATAA CATGAACAGT TCGTTCTCCT ACCCTGGGAA CTATGCAAGA 
GGTCGCATCC CTGGCAACAG TCATCCTCCT GCACAGAACC CCATCAATGG CTTTCACCCT 
AACCTCCAAG ATATACGATA TGGTTATTAC AACTACCCTT CCAGTGCACT TTTCTCTCCT 
GAGCTCTTGA GATATGATAA AGGAGCCTGG CCCAAAAACG GAGAACCCTC TGCCCAAGCA 
TTTGAACAGA AGCCGGATGT TCAGTGTCTC CAAGCCAATC TGGCACAGGC CTATCCCACC 
CATCATGGAC AGTCTCAGGC ACAAATGGCT GATGCATCCA TTCAAGGCTA CCCACAACCC 
TCTGATATTT CGCAGTGTCG TACTCCATCT ATGTCTCTTG AGCAGACACA CCGCTCAAAA 
CCCATAGTCA AGCAGGAGCC CATGGATGTA CCATTGTATG AAGCAAGATC AGATGGTCAA 
GCGCAGAGCT GCCCGACCAC TCCTAGTACA ACACCAACAC CTGAAGGATG GCATGGGCAC 
AAACCAAACG GCAGTCTAGT ACCTAAAGGC TGGGATGGAA ACATCAGAGC GGCCTCAAAT 
AATTCACCCT TCACACCAGA TAAGCAGAGG CTCCACCAGC AACACCAACA CGAACACCCT 
CAATATCCAC AGCAGCAACA GTGGAATTCC TATTCCTGCC CAAACACTCC AATGGCATCT 
CCTGCCCCAT CTCCGTCCCC ATCCCTAAAA GCAGGTCTAT CACCTGCACC CTCTCCACAC 
CCGTCCACCC CAAGGCAGTG GGGAAGTCCT GCCCCTAGTC CACAACCTAA AGCATGGGGT 
CCCTATGGTC CCATGGGTTA TGGTGCTGGT GTTATCAGGC AAGGCAACCC TGCTGGTGCT 
TACCCTGATA ACATGCTTTC CCAGGCAGGT GTAAATTGTG GCTCAGCACC CCTTGGACTC 
CAGGAGAAAG CCTGGAAGTC TGGTGGTGGG TCAGCAGCAG GTAGCACGCC ATCCCCAGCT 
CCTGAGGGCC GATTGTTTCC CGATGCACTG CAAAGGTCGG ACGGCCAGGC ATGTTGGGAC 
AGTGGAGCAG AGACACAAAG TGAACGAGAT CCTGAAGAGG ACGAGGTTTG GTCGGACAGT 
GAACACAACT TCTTGGATCC CAACATCGGA GGAGTAGCAG TTGCACCTGC TCATGGTTGT 
GTCTTGATTG AGTGTGCCCG TCGAGAACTC CATGCCACCA CACCGCTTAA AAAGCCTGAC 
CGCACTCACC CAAGCCGCAT ATCCCTGGTC TTCTACCAGC ACAAGAACCT GAACCAGCCC 
TGCCATGGTC TGGCTTTGTG GGAATCCAAG ATGAAAATCC TGGCAGAGCG AGCGCGACAA 
CGGCAACAAG AAGCTGCTCT TTTGGGTCTC TCACAGGAAG AAATCAAGGC CTACGGGAAA 
AAGCGCAAAT GGGCGGCTGG GTCAGCAAGC CCGTCCCCTG GACAAACTAA GGACAAACGG 
GAGGGTGTAG TGACACGGAT GGCCCCAACA CAGCATAGCA CCACCATAGT CACAGTTTCA 
CCTTACCCGT CCACACACCT CACTGGTCCT TACAGTCGAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tet3 ( XM_009301229.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299504.1 Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10183
Clone ID Related Accession (Same CDS sequence)	XM_003198607.5 , XM_009301229.2 , XM_003198607.4 , NM_001327946.1 , XM_009301229.3
Accession Version	XM_009301229.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	methylcytosine dioxygenase TET3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATTG GGTCCCATGA ACGCCTGGAA GAAGGCTTAT TAAACCAGGA ACTCTCTAAT 
GGGCTTAGCA AACATGCATC TAATGAAGAA AATGTTTGTG ATAATGAAGA CAGAGATGCA 
ACCAAGGAGC AACACCAGCA TAACTTTCAT GAGGGGGCTG GAAGTATTTC CCAGACACCA 
GGCTGGGTCC TTGAGCATAC TGGGAAGTTG TCAAATCCTG AATCTCAACA AGGTGTTTGG 
AGTGGTCAAA ATGCCAGCAC AACAGTAAAT GATGTGGATA TGGAGGATGC ACAAACTCTG 
GTGGCCTTTT CTGTTGGCTC ACTGCCACCT TACACCAACC ACCAACCTTC ACCGACCCCT 
ACAGCTCAGC TGTATGAAAG ATTCAATCAG GAAATGTCCA ATGGAGGTGA GGAGGCCAAA 
AAGAAAGGTG TTGATGGTGC GTTTTGCCCC CCTGAAGATC TAAACACTCT GCAGGCAGCA 
CTGAGCAAGG CCCGTCATGG CCACAAGCCA CCTAATTGTG ATTGTGATGG CCCAGACTGC 
CCTGATTACC TGGAGTGGCT AGAGAAGCAA ATTAAATTGG TGACTAACTG TCAGAATAAA 
ACTTCCTGTA ATCTTTCGGG TACGGCTCAA CAGCCACTTG AACAGCATCA TTATTCCCAA 
TCCCAACCTC AACAATGTGG AGGTAAACCA TTGTGTGTGC AAGACACAAA TCCACAGCCC 
CTTGTTAACA AGCCTTCTAC TCCTAGACCC TTTGGCCCAC CAGCACCTAT TCCCTGCTCC 
CCTAGTGTTC TGTCCATAGC CAAGGAAAGA AATGTTAGTT TGCAAACAGC CATTGCAATT 
GAGGCTCTGA CCCAGTTGTC TGCCACTGTC CCACAAACTG TTGTACCTCC TGGTGAGTCT 
TCACTTGCAA ATCACCATCA GCATCTGCCA ACCTCCTACC ACCAGAACAT GCCACAAGTT 
GTTTCTTCAT CACCTGGTCC TCTGTTGTCC TCCTCTGCCA CCTCTGCTAT TCCACAGTCA 
AATCATTACC CTCAACAAGA GCTTACAACA TGGGAGCAAC AGAGACCTCA GTCTAAGGGT 
GAGGCCCCTA CAGCAAACAT TTTTCCTAGC TCCTCTCATT ATTCTTCCTC ATACCCATCT 
TCCAAGCCCC CATTTACAAA TATGCACGCA TCAAGCCCCA ATCCTCGACC TCAGCAGTGG 
AACCAGGGCA CAGCTAGTAG CTGTGAGAAA AACTCCCCTC GCAATGCATG GATGATGGCA 
AACACAGACT CTAAACAACA TTTTGCAAAG CCATCCCATG AGAGCAGTGA CCCAGTGTCT 
GAGCTGAAGC AACTTCTTGG AGACACCGGT GGAAAGCACC CGTTCAAGTT CCCATTCCCT 
CACCCCAGCT TACAGGATGT TCATAAGGGC AGCTTTACTG GGATGCCTCA CATAAAGCAG 
GAGGTGGAAA CAAAAGAATA CCTGGGATCA GAATGCTCAG TCATGGGCCA GGAAGGGATG 
CTAAATGGCC AGCAGCAGTT TCAGGGTCAG CAGTTATCCT CCACCATTAG GCACTCAACT 
CAGGCAGCAC TACAGCAGCA CCTTCATCAT AAACGTAATC TCTTCTCTAA TTCCCCAACA 
TATGGTCCAT TAGCCCACAT GGCTTGTCAG GACCTTCGTA AATGGTGGCC TCAGACTATT 
CCAGAGAATC TCATTAACAT CAAACAGGAA CACAAGGAAC CTAAAAAGAA AAAGGGTGCT 
CAAAGTTCTC ATCTAAAGCA GCCAGCTGGT GGGCCTTTTG GTCCACTGGG CACACCTCTT 
CCAAAACCCA AACCGATTGT TATCAAGAAG CATAAACAAA AAGCTTCCCA GCCAACTTTT 
CTACCACAGA TCCAGATTAC AATATCAAAA CCACCTCTCA TTTCTCCAGG TTATGCACCA 
TCCCGAGCTC AGCTTGTTCC ATCTTTGCCA GGCATGGAAG CTGGGCTTCC CCCCACTCAG 
GTGGCTGAGA GCCACCCAGC TCCAGCTCAA TCTCAGGAAT CCATTATAAA TTGCAGTAGT 
GCACCCATCT CTGAAAATAG TCATTCTTCT ACTAACCCTG TGTCAGTCCT AGCACCATCA 
ACCCAGAAGG TAATAGCATG CCCAGGCTCA GCTGGATCAA ATGACAGCAT CCCAACAACC 
AGTATCACAC CTCAAACATC TACAACACAG CCATCTCCTT CTCTGGCTGG CCTCAGTAAC 
CTAGATCCAA AATTTGAAGA GCTGATTCGG CAGTTTGAGG AGGAGTTTGG GGACACTGCA 
GCTTCTGCAG CCGAGACCCC AGAGAATGGC CCTGTTCAAC CAGTGGTGAC CGAGTCGCAC 
CCAAACTCAG GACAATTGAG ACCCGAGTCA CCATCCCTAA AGCAGCCAAA CAACTGCCCT 
CCTGTTCCCA CAAATGAGTC TGAGCCCATG AACGAAGGTC AAAAGGACAT ACCTGAAAGA 
CAGAAGGAAA AAACACCATT GCCACCTGAG GAGCTCTGTA CTGTCAAACA GGAAAGTGAG 
GAATGTGCAT TGGATGTGAA ACCTTCAGGG GAGCTCCTGT CTGAATTTCT GAAGCAACAG 
AACCCCTTCG TTGCACCTTT TTCTCCTCCA AATAAACGTA TGAAGATTGA ATCATCCAAT 
GGTGTGACCG TGCTCTCCAC AACTGCATGT TTCTCTGCAG ACAGAGAGGG TGATGATTTC 
AATACTCCCA CCAAGGATAT CTTTCCCTCC TCACCTTCAC TTAAAGGTTT TCTTGAGTCA 
CCATTGCGCT ACCTGGACAC TCCCACCAAG AACCTGCTTG ATACACCTGG GAAAGATGTT 
CAACCTGATT TTCCAATCTG TGATTGCGTA GACCAAGTCC TTGAAAAGGA TGAAGGTCCA 
TATTACAATC ATTTGGGATC TGGACGAGAT ATACCTTCAG TCAGACAACT GATGGAAGAT 
AGGTATGGGG AGAAGGGGGA AGCCGTTCGC ATTGAAAAGG TGGTTTACAC AGGGCGAGAA 
GGAAAGAGCT CTCAGGGATG TCCTATTGCT AAGTGGGTGT TACGTCGCAG CAGTGAAAAG 
GAGAAAGTGT TGTGCGTTGT GAAGCAACGG CCTGGGCACC ACTGTGCCAA CACTGTCATC 
GTTGTTGTCA TCCTGGCCTG GGAAGGTGTG CCCCGTGCTC TGGGAGATAA ACTGTACAGA 
GAAGTCACAG AAACCATCAC CAAATATGGC AATCCCACCA GCCGTCGCTG TGGACTCAAT 
GAAGATCGAA CCTGTGCATG TCAAGGGAAG GACCCAGAGA CCTGTGGAGC TTCATTCTCT 
TTTGGCTGTT CCTGGAGCAT GTATTTCAAT GGATGCAAGT ATGCTCGAAG CAAGGTCCCT 
CGTAAGTTCA GACTGCAGGG AGAGCACCCC AAAGAGGAGG ACAATCTCAG GGATAACTTC 
CAAGCTTTGG CCACACATGT GGCCCCTCTG TATAAGAAGC TAGCTCCCCA GGCCTACAGC 
AACCAGTGTT TACATGAGGA TGTTGCTTCA GACTGTCGGT TGGGATTGAA GGAAGGACGT 
CCATTTTCAG GAATCACTGC ATGCATGGAT TTCTGTGCCC ACGCTCACAA AGACCAGCAT 
AACCTCCACA ATGGCTGCAC TGTGGTGTGC ACTCTCACGA AAGAGGACAA TCGTACAGTG 
GGCACAATCC CAGAAGATGA GCAGCTGCAC GTGCTTCCGC TCTACAAGCT CGCTACAACC 
GACGAGTTTG GTAGTGAGGA GAACCAGCGT CTCAAAATGC AGACAGGAGC CATCCAGGTG 
CTCGCTAACT TCCGTCGAGA GGTTAGAAAA CTTCCAGAAC CTGCAAAATC ATGCCGCCAG 
CGTCGGTTGG AGGCCAAAAA GGCTGCATCA GAGAAAAAGA ACAGGAAATT GCAGCTAGCA 
GAAACTCCAG AGAAGACCGT CAAGATGGAG ATTCGCCATG CAGAGTCACC TCACATTCAG 
CAAGGCAATA AAGCTATTCC AAAACAGGAG GTGAAACCAA CAATTAAAAA AGAGCCTGTC 
GACCACTTCC AGCCCTTCAA TGGAGCCATA CATGGTTACC AAGCACTAGG GAATGGCAAG 
ACAAACCCTG ATCCCCATAA CATGAACAGT TCGTTCTCCT ACCCTGGGAA CTATGCAAGA 
GGTCGCATCC CTGGCAACAG TCATCCTCCT GCACAGAACC CCATCAATGG CTTTCACCCT 
AACCTCCAAG ATATACGATA TGGTTATTAC AACTACCCTT CCAGTGCACT TTTCTCTCCT 
GAGCTCTTGA GATATGATAA AGGAGCCTGG CCCAAAAACG GAGAACCCTC TGCCCAAGCA 
TTTGAACAGA AGCCGGATGT TCAGTGTCTC CAAGCCAATC TGGCACAGGC CTATCCCACC 
CATCATGGAC AGTCTCAGGC ACAAATGGCT GATGCATCCA TTCAAGGCTA CCCACAACCC 
TCTGATATTT CGCAGTGTCG TACTCCATCT ATGTCTCTTG AGCAGACACA CCGCTCAAAA 
CCCATAGTCA AGCAGGAGCC CATGGATGTA CCATTGTATG AAGCAAGATC AGATGGTCAA 
GCGCAGAGCT GCCCGACCAC TCCTAGTACA ACACCAACAC CTGAAGGATG GCATGGGCAC 
AAACCAAACG GCAGTCTAGT ACCTAAAGGC TGGGATGGAA ACATCAGAGC GGCCTCAAAT 
AATTCACCCT TCACACCAGA TAAGCAGAGG CTCCACCAGC AACACCAACA CGAACACCCT 
CAATATCCAC AGCAGCAACA GTGGAATTCC TATTCCTGCC CAAACACTCC AATGGCATCT 
CCTGCCCCAT CTCCGTCCCC ATCCCTAAAA GCAGGTCTAT CACCTGCACC CTCTCCACAC 
CCGTCCACCC CAAGGCAGTG GGGAAGTCCT GCCCCTAGTC CACAACCTAA AGCATGGGGT 
CCCTATGGTC CCATGGGTTA TGGTGCTGGT GTTATCAGGC AAGGCAACCC TGCTGGTGCT 
TACCCTGATA ACATGCTTTC CCAGGCAGGT GTAAATTGTG GCTCAGCACC CCTTGGACTC 
CAGGAGAAAG CCTGGAAGTC TGGTGGTGGG TCAGCAGCAG GTAGCACGCC ATCCCCAGCT 
CCTGAGGGCC GATTGTTTCC CGATGCACTG CAAAGGTCGG ACGGCCAGGC ATGTTGGGAC 
AGTGGAGCAG AGACACAAAG TGAACGAGAT CCTGAAGAGG ACGAGGTTTG GTCGGACAGT 
GAACACAACT TCTTGGATCC CAACATCGGA GGAGTAGCAG TTGCACCTGC TCATGGTTGT 
GTCTTGATTG AGTGTGCCCG TCGAGAACTC CATGCCACCA CACCGCTTAA AAAGCCTGAC 
CGCACTCACC CAAGCCGCAT ATCCCTGGTC TTCTACCAGC ACAAGAACCT GAACCAGCCC 
TGCCATGGTC TGGCTTTGTG GGAATCCAAG ATGAAAATCC TGGCAGAGCG AGCGCGACAA 
CGGCAACAAG AAGCTGCTCT TTTGGGTCTC TCACAGGAAG AAATCAAGGC CTACGGGAAA 
AAGCGCAAAT GGGCGGCTGG GTCAGCAAGC CCGTCCCCTG GACAAACTAA GGACAAACGG 
GAGGGTGTAG TGACACGGAT GGCCCCAACA CAGCATAGCA CCACCATAGT CACAGTTTCA 
CCTTACCCGT CCACACACCT CACTGGTCCT TACAGTCGAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299504.1
CDS	539..6226
Translation	MEIGSHERLEEGLLNQELSNGLSKHASNEENVCDNEDRDATKEQHQHNFHEGAGSISQTPGWVLEHTGKLSNPESQQGVWSGQNASTTVNDVDMEDAQTLVAFSVGSLPPYTNHQPSPTPTAQLYERFNQEMSNGGEEAKKKGVDGAFCPPEDLNTLQAALSKARHGHKPPNCDCDGPDCPDYLEWLEKQIKLVTNCQNKTSCNLSGTAQQPLEQHHYSQSQPQQCGGKPLCVQDTNPQPLVNKPSTPRPFGPPAPIPCSPSVLSIAKERNVSLQTAIAIEALTQLSATVPQTVVPPGESSLANHHQHLPTSYHQNMPQVVSSSPGPLLSSSATSAIPQSNHYPQQELTTWEQQRPQSKGEAPTANIFPSSSHYSSSYPSSKPPFTNMHASSPNPRPQQWNQGTASSCEKNSPRNAWMMANTDSKQHFAKPSHESSDPVSELKQLLGDTGGKHPFKFPFPHPSLQDVHKGSFTGMPHIKQEVETKEYLGSECSVMGQEGMLNGQQQFQGQQLSSTIRHSTQAALQQHLHHKRNLFSNSPTYGPLAHMACQDLRKWWPQTIPENLINIKQEHKEPKKKKGAQSSHLKQPAGGPFGPLGTPLPKPKPIVIKKHKQKASQPTFLPQIQITISKPPLISPGYAPSRAQLVPSLPGMEAGLPPTQVAESHPAPAQSQESIINCSSAPISENSHSSTNPVSVLAPSTQKVIACPGSAGSNDSIPTTSITPQTSTTQPSPSLAGLSNLDPKFEELIRQFEEEFGDTAASAAETPENGPVQPVVTESHPNSGQLRPESPSLKQPNNCPPVPTNESEPMNEGQKDIPERQKEKTPLPPEELCTVKQESEECALDVKPSGELLSEFLKQQNPFVAPFSPPNKRMKIESSNGVTVLSTTACFSADREGDDFNTPTKDIFPSSPSLKGFLESPLRYLDTPTKNLLDTPGKDVQPDFPICDCVDQVLEKDEGPYYNHLGSGRDIPSVRQLMEDRYGEKGEAVRIEKVVYTGREGKSSQGCPIAKWVLRRSSEKEKVLCVVKQRPGHHCANTVIVVVILAWEGVPRALGDKLYREVTETITKYGNPTSRRCGLNEDRTCACQGKDPETCGASFSFGCSWSMYFNGCKYARSKVPRKFRLQGEHPKEEDNLRDNFQALATHVAPLYKKLAPQAYSNQCLHEDVASDCRLGLKEGRPFSGITACMDFCAHAHKDQHNLHNGCTVVCTLTKEDNRTVGTIPEDEQLHVLPLYKLATTDEFGSEENQRLKMQTGAIQVLANFRREVRKLPEPAKSCRQRRLEAKKAASEKKNRKLQLAETPEKTVKMEIRHAESPHIQQGNKAIPKQEVKPTIKKEPVDHFQPFNGAIHGYQALGNGKTNPDPHNMNSSFSYPGNYARGRIPGNSHPPAQNPINGFHPNLQDIRYGYYNYPSSALFSPELLRYDKGAWPKNGEPSAQAFEQKPDVQCLQANLAQAYPTHHGQSQAQMADASIQGYPQPSDISQCRTPSMSLEQTHRSKPIVKQEPMDVPLYEARSDGQAQSCPTTPSTTPTPEGWHGHKPNGSLVPKGWDGNIRAASNNSPFTPDKQRLHQQHQHEHPQYPQQQQWNSYSCPNTPMASPAPSPSPSLKAGLSPAPSPHPSTPRQWGSPAPSPQPKAWGPYGPMGYGAGVIRQGNPAGAYPDNMLSQAGVNCGSAPLGLQEKAWKSGGGSAAGSTPSPAPEGRLFPDALQRSDGQACWDSGAETQSERDPEEDEVWSDSEHNFLDPNIGGVAVAPAHGCVLIECARRELHATTPLKKPDRTHPSRISLVFYQHKNLNQPCHGLALWESKMKILAERARQRQQEAALLGLSQEEIKAYGKKRKWAAGSASPSPGQTKDKREGVVTRMAPTQHSTTIVTVSPYPSTHLTGPYSRFV

Target ORF information:

RefSeq Version	XM_009301229.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301229.3

ORF Insert Sequence:

ATGGAAATTG GGTCCCATGA ACGCCTGGAA GAAGGCTTAT TAAACCAGGA ACTCTCTAAT 
GGGCTTAGCA AACATGCATC TAATGAAGAA AATGTTTGTG ATAATGAAGA CAGAGATGCA 
ACCAAGGAGC AACACCAGCA TAACTTTCAT GAGGGGGCTG GAAGTATTTC CCAGACACCA 
GGCTGGGTCC TTGAGCATAC TGGGAAGTTG TCAAATCCTG AATCTCAACA AGGTGTTTGG 
AGTGGTCAAA ATGCCAGCAC AACAGTAAAT GATGTGGATA TGGAGGATGC ACAAACTCTG 
GTGGCCTTTT CTGTTGGCTC ACTGCCACCT TACACCAACC ACCAACCTTC ACCGACCCCT 
ACAGCTCAGC TGTATGAAAG ATTCAATCAG GAAATGTCCA ATGGAGGTGA GGAGGCCAAA 
AAGAAAGGTG TTGATGGTGC GTTTTGCCCC CCTGAAGATC TAAACACTCT GCAGGCAGCA 
CTGAGCAAGG CCCGTCATGG CCACAAGCCA CCTAATTGTG ATTGTGATGG CCCAGACTGC 
CCTGATTACC TGGAGTGGCT AGAGAAGCAA ATTAAATTGG TGACTAACTG TCAGAATAAA 
ACTTCCTGTA ATCTTTCGGG TACGGCTCAA CAGCCACTTG AACAGCATCA TTATTCCCAA 
TCCCAACCTC AACAATGTGG AGGTAAACCA TTGTGTGTGC AAGACACAAA TCCACAGCCC 
CTTGTTAACA AGCCTTCTAC TCCTAGACCC TTTGGCCCAC CAGCACCTAT TCCCTGCTCC 
CCTAGTGTTC TGTCCATAGC CAAGGAAAGA AATGTTAGTT TGCAAACAGC CATTGCAATT 
GAGGCTCTGA CCCAGTTGTC TGCCACTGTC CCACAAACTG TTGTACCTCC TGGTGAGTCT 
TCACTTGCAA ATCACCATCA GCATCTGCCA ACCTCCTACC ACCAGAACAT GCCACAAGTT 
GTTTCTTCAT CACCTGGTCC TCTGTTGTCC TCCTCTGCCA CCTCTGCTAT TCCACAGTCA 
AATCATTACC CTCAACAAGA GCTTACAACA TGGGAGCAAC AGAGACCTCA GTCTAAGGGT 
GAGGCCCCTA CAGCAAACAT TTTTCCTAGC TCCTCTCATT ATTCTTCCTC ATACCCATCT 
TCCAAGCCCC CATTTACAAA TATGCACGCA TCAAGCCCCA ATCCTCGACC TCAGCAGTGG 
AACCAGGGCA CAGCTAGTAG CTGTGAGAAA AACTCCCCTC GCAATGCATG GATGATGGCA 
AACACAGACT CTAAACAACA TTTTGCAAAG CCATCCCATG AGAGCAGTGA CCCAGTGTCT 
GAGCTGAAGC AACTTCTTGG AGACACCGGT GGAAAGCACC CGTTCAAGTT CCCATTCCCT 
CACCCCAGCT TACAGGATGT TCATAAGGGC AGCTTTACTG GGATGCCTCA CATAAAGCAG 
GAGGTGGAAA CAAAAGAATA CCTGGGATCA GAATGCTCAG TCATGGGCCA GGAAGGGATG 
CTAAATGGCC AGCAGCAGTT TCAGGGTCAG CAGTTATCCT CCACCATTAG GCACTCAACT 
CAGGCAGCAC TACAGCAGCA CCTTCATCAT AAACGTAATC TCTTCTCTAA TTCCCCAACA 
TATGGTCCAT TAGCCCACAT GGCTTGTCAG GACCTTCGTA AATGGTGGCC TCAGACTATT 
CCAGAGAATC TCATTAACAT CAAACAGGAA CACAAGGAAC CTAAAAAGAA AAAGGGTGCT 
CAAAGTTCTC ATCTAAAGCA GCCAGCTGGT GGGCCTTTTG GTCCACTGGG CACACCTCTT 
CCAAAACCCA AACCGATTGT TATCAAGAAG CATAAACAAA AAGCTTCCCA GCCAACTTTT 
CTACCACAGA TCCAGATTAC AATATCAAAA CCACCTCTCA TTTCTCCAGG TTATGCACCA 
TCCCGAGCTC AGCTTGTTCC ATCTTTGCCA GGCATGGAAG CTGGGCTTCC CCCCACTCAG 
GTGGCTGAGA GCCACCCAGC TCCAGCTCAA TCTCAGGAAT CCATTATAAA TTGCAGTAGT 
GCACCCATCT CTGAAAATAG TCATTCTTCT ACTAACCCTG TGTCAGTCCT AGCACCATCA 
ACCCAGAAGG TAATAGCATG CCCAGGCTCA GCTGGATCAA ATGACAGCAT CCCAACAACC 
AGTATCACAC CTCAAACATC TACAACACAG CCATCTCCTT CTCTGGCTGG CCTCAGTAAC 
CTAGATCCAA AATTTGAAGA GCTGATTCGG CAGTTTGAGG AGGAGTTTGG GGACACTGCA 
GCTTCTGCAG CCGAGACCCC AGAGAATGGC CCTGTTCAAC CAGTGGTGAC CGAGTCGCAC 
CCAAACTCAG GACAATTGAG ACCCGAGTCA CCATCCCTAA AGCAGCCAAA CAACTGCCCT 
CCTGTTCCCA CAAATGAGTC TGAGCCCATG AACGAAGGTC AAAAGGACAT ACCTGAAAGA 
CAGAAGGAAA AAACACCATT GCCACCTGAG GAGCTCTGTA CTGTCAAACA GGAAAGTGAG 
GAATGTGCAT TGGATGTGAA ACCTTCAGGG GAGCTCCTGT CTGAATTTCT GAAGCAACAG 
AACCCCTTCG TTGCACCTTT TTCTCCTCCA AATAAACGTA TGAAGATTGA ATCATCCAAT 
GGTGTGACCG TGCTCTCCAC AACTGCATGT TTCTCTGCAG ACAGAGAGGG TGATGATTTC 
AATACTCCCA CCAAGGATAT CTTTCCCTCC TCACCTTCAC TTAAAGGTTT TCTTGAGTCA 
CCATTGCGCT ACCTGGACAC TCCCACCAAG AACCTGCTTG ATACACCTGG GAAAGATGTT 
CAACCTGATT TTCCAATCTG TGATTGCGTA GACCAAGTCC TTGAAAAGGA TGAAGGTCCA 
TATTACAATC ATTTGGGATC TGGACGAGAT ATACCTTCAG TCAGACAACT GATGGAAGAT 
AGGTATGGGG AGAAGGGGGA AGCCGTTCGC ATTGAAAAGG TGGTTTACAC AGGGCGAGAA 
GGAAAGAGCT CTCAGGGATG TCCTATTGCT AAGTGGGTGT TACGTCGCAG CAGTGAAAAG 
GAGAAAGTGT TGTGCGTTGT GAAGCAACGG CCTGGGCACC ACTGTGCCAA CACTGTCATC 
GTTGTTGTCA TCCTGGCCTG GGAAGGTGTG CCCCGTGCTC TGGGAGATAA ACTGTACAGA 
GAAGTCACAG AAACCATCAC CAAATATGGC AATCCCACCA GCCGTCGCTG TGGACTCAAT 
GAAGATCGAA CCTGTGCATG TCAAGGGAAG GACCCAGAGA CCTGTGGAGC TTCATTCTCT 
TTTGGCTGTT CCTGGAGCAT GTATTTCAAT GGATGCAAGT ATGCTCGAAG CAAGGTCCCT 
CGTAAGTTCA GACTGCAGGG AGAGCACCCC AAAGAGGAGG ACAATCTCAG GGATAACTTC 
CAAGCTTTGG CCACACATGT GGCCCCTCTG TATAAGAAGC TAGCTCCCCA GGCCTACAGC 
AACCAGTGTT TACATGAGGA TGTTGCTTCA GACTGTCGGT TGGGATTGAA GGAAGGACGT 
CCATTTTCAG GAATCACTGC ATGCATGGAT TTCTGTGCCC ACGCTCACAA AGACCAGCAT 
AACCTCCACA ATGGCTGCAC TGTGGTGTGC ACTCTCACGA AAGAGGACAA TCGTACAGTG 
GGCACAATCC CAGAAGATGA GCAGCTGCAC GTGCTTCCGC TCTACAAGCT CGCTACAACC 
GACGAGTTTG GTAGTGAGGA GAACCAGCGT CTCAAAATGC AGACAGGAGC CATCCAGGTG 
CTCGCTAACT TCCGTCGAGA GGTTAGAAAA CTTCCAGAAC CTGCAAAATC ATGCCGCCAG 
CGTCGGTTGG AGGCCAAAAA GGCTGCATCA GAGAAAAAGA ACAGGAAATT GCAGCTAGCA 
GAAACTCCAG AGAAGACCGT CAAGATGGAG ATTCGCCATG CAGAGTCACC TCACATTCAG 
CAAGGCAATA AAGCTATTCC AAAACAGGAG GTGAAACCAA CAATTAAAAA AGAGCCTGTC 
GACCACTTCC AGCCCTTCAA TGGAGCCATA CATGGTTACC AAGCACTAGG GAATGGCAAG 
ACAAACCCTG ATCCCCATAA CATGAACAGT TCGTTCTCCT ACCCTGGGAA CTATGCAAGA 
GGTCGCATCC CTGGCAACAG TCATCCTCCT GCACAGAACC CCATCAATGG CTTTCACCCT 
AACCTCCAAG ATATACGATA TGGTTATTAC AACTACCCTT CCAGTGCACT TTTCTCTCCT 
GAGCTCTTGA GATATGATAA AGGAGCCTGG CCCAAAAACG GAGAACCCTC TGCCCAAGCA 
TTTGAACAGA AGCCGGATGT TCAGTGTCTC CAAGCCAATC TGGCACAGGC CTATCCCACC 
CATCATGGAC AGTCTCAGGC ACAAATGGCT GATGCATCCA TTCAAGGCTA CCCACAACCC 
TCTGATATTT CGCAGTGTCG TACTCCATCT ATGTCTCTTG AGCAGACACA CCGCTCAAAA 
CCCATAGTCA AGCAGGAGCC CATGGATGTA CCATTGTATG AAGCAAGATC AGATGGTCAA 
GCGCAGAGCT GCCCGACCAC TCCTAGTACA ACACCAACAC CTGAAGGATG GCATGGGCAC 
AAACCAAACG GCAGTCTAGT ACCTAAAGGC TGGGATGGAA ACATCAGAGC GGCCTCAAAT 
AATTCACCCT TCACACCAGA TAAGCAGAGG CTCCACCAGC AACACCAACA CGAACACCCT 
CAATATCCAC AGCAGCAACA GTGGAATTCC TATTCCTGCC CAAACACTCC AATGGCATCT 
CCTGCCCCAT CTCCGTCCCC ATCCCTAAAA GCAGGTCTAT CACCTGCACC CTCTCCACAC 
CCGTCCACCC CAAGGCAGTG GGGAAGTCCT GCCCCTAGTC CACAACCTAA AGCATGGGGT 
CCCTATGGTC CCATGGGTTA TGGTGCTGGT GTTATCAGGC AAGGCAACCC TGCTGGTGCT 
TACCCTGATA ACATGCTTTC CCAGGCAGGT GTAAATTGTG GCTCAGCACC CCTTGGACTC 
CAGGAGAAAG CCTGGAAGTC TGGTGGTGGG TCAGCAGCAG GTAGCACGCC ATCCCCAGCT 
CCTGAGGGCC GATTGTTTCC CGATGCACTG CAAAGGTCGG ACGGCCAGGC ATGTTGGGAC 
AGTGGAGCAG AGACACAAAG TGAACGAGAT CCTGAAGAGG ACGAGGTTTG GTCGGACAGT 
GAACACAACT TCTTGGATCC CAACATCGGA GGAGTAGCAG TTGCACCTGC TCATGGTTGT 
GTCTTGATTG AGTGTGCCCG TCGAGAACTC CATGCCACCA CACCGCTTAA AAAGCCTGAC 
CGCACTCACC CAAGCCGCAT ATCCCTGGTC TTCTACCAGC ACAAGAACCT GAACCAGCCC 
TGCCATGGTC TGGCTTTGTG GGAATCCAAG ATGAAAATCC TGGCAGAGCG AGCGCGACAA 
CGGCAACAAG AAGCTGCTCT TTTGGGTCTC TCACAGGAAG AAATCAAGGC CTACGGGAAA 
AAGCGCAAAT GGGCGGCTGG GTCAGCAAGC CCGTCCCCTG GACAAACTAA GGACAAACGG 
GAGGGTGTAG TGACACGGAT GGCCCCAACA CAGCATAGCA CCACCATAGT CACAGTTTCA 
CCTTACCCGT CCACACACCT CACTGGTCCT TACAGTCGAT TTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tet3 ( NM_001327945.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001314874.1 Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant 1, mRNA.

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CloneID	ODa10182
Clone ID Related Accession (Same CDS sequence)	NM_001327945.1
Accession Version	NM_001327945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-12
Organism	Danio rerio(zebrafish)
Product	methylcytosine dioxygenase TET3 isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT009570.9 and CR388049.7. On May 28, 2016 this sequence version replaced XM_005168616.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01024896.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCACAAGC TGAGGGGAGG CCCTGGCCCT CCAGTCCAGG ACATTTATGA ATTCTCTCTG 
GACGAGGAGG ACCCTAAAGT TTTCCTAGGG GCCAGGAGCG CAAGGGTGGT GTCGCCTGCG 
GACGACTACA TCAGGTCTGA GCATGAGCAA GCTGGCATGT TCTCCTTGTC GCAGATGGAG 
GACCAGGAAC CAATAGAGCC TGTGGTGGCC CAAAAAAAGA AAAGGAAGAG ATGCGGCGTT 
TGTGGGCCAT GCATGAGGAA GGAGAACTGC GGGACCTGTA GCAATTGCCT AAACCGAAAG 
ATCGGACACC AGATCTGCAA ACTGAGAAAG TGTGATGAGT TGAAGAGGAA GAAGAGCCCG 
TGGGAGGTGG AATATTTTGA AGACAAAAAT ACCCAGCCTG TAATCCCAAA CCTGGCTAAA 
GAGACTGTTT CTGTGGTGGG TTCAGTGGAT GGCCCCAGAG GAAAAGGCCA GATGGAAATT 
GGGTCCCATG AACGCCTGGA AGAAGGCTTA TTAAACCAGG AACTCTCTAA TGGGCTTAGC 
AAACATGCAT CTAATGAAGA AAATGTTTGT GATAATGAAG ACAGAGATGC AACCAAGGAG 
CAACACCAGC ATAACTTTCA TGAGGGGGCT GGAAGTATTT CCCAGACACC AGGCTGGGTC 
CTTGAGCATA CTGGGAAGTT GTCAAATCCT GAATCTCAAC AAGGTGTTTG GAGTGGTCAA 
AATGCCAGCA CAACAGTAAA TGATGTGGAT ATGGAGGATG CACAAACTCT GGTGGCCTTT 
TCTGTTGGCT CACTGCCACC TTACACCAAC CACCAACCTT CACCGACCCC TACAGCTCAG 
CTGTATGAAA GATTCAATCA GGAAATGTCC AATGGAGGTG AGGAGGCCAA AAAGAAAGGT 
GTTGATGGTG CGTTTTGCCC CCCTGAAGAT CTAAACACTC TGCAGGCAGC ACTGAGCAAG 
GCCCGTCATG GCCACAAGCC ACCTAATTGT GATTGTGATG GCCCAGACTG CCCTGATTAC 
CTGGAGTGGC TAGAGAAGCA AATTAAATTG GTGACTAACT GTCAGAATAA AACTTCCTGT 
AATCTTTCGG GTACGGCTCA ACAGCCACTT GAACAGCATC ATTATTCCCA ATCCCAACCT 
CAACAATGTG GAGGTAAACC ATTGTGTGTG CAAGACACAA ATCCACAGCC CCTTGTTAAC 
AAGCCTTCTA CTCCTAGACC CTTTGGCCCA CCAGCACCTA TTCCCTGCTC CCCTAGTGTT 
CTGTCCATAG CCAAGGAAAG AAATGTTAGT TTGCAAACAG CCATTGCAAT TGAGGCTCTG 
ACCCAGTTGT CTGCCACTGT CCCACAAACT GTTGTACCTC CTGGTGAGTC TTCACTTGCA 
AATCACCATC AGCATCTGCC AACCTCCTAC CACCAGAACA TGCCACAAGT TGTTTCTTCA 
TCACCTGGTC CTCTGTTGTC CTCCTCTGCC ACCTCTGCTA TTCCACAGTC AAATCATTAC 
CCTCAACAAG AGCTTACAAC ATGGGAGCAA CAGAGACCTC AGTCTAAGGG TGAGGCCCCT 
ACAGCAAACA TTTTTCCTAG CTCCTCTCAT TATTCTTCCT CATACCCATC TTCCAAGCCC 
CCATTTACAA ATATGCACGC ATCAAGCCCC AATCCTCGAC CTCAGCAGTG GAACCAGGGC 
ACAGCTAGTA GCTGTGAGAA AAACTCCCCT CGCAATGCAT GGATGATGGC AAACACAGAC 
TCTAAACAAC ATTTTGCAAA GCCATCCCAT GAGAGCAGTG ACCCAGTGTC TGAGCTGAAG 
CAACTTCTTG GAGACACCGG TGGAAAGCAC CCGTTCAAGT TCCCATTCCC TCACCCCAGC 
TTACAGGATG TTCATAAGGG CAGCTTTACT GGGATGCCTC ACATAAAGCA GGAGGTGGAA 
ACAAAAGAAT ACCTGGGATC AGAATGCTCA GTCATGGGCC AGGAAGGGAT GCTAAATGGC 
CAGCAGCAGT TTCAGGGTCA GCAGTTATCC TCCACCATTA GGCACTCAAC TCAGGCAGCA 
CTACAGCAGC ACCTTCATCA TAAACGTAAT CTCTTCTCTA ATTCCCCAAC ATATGGTCCA 
TTAGCCCACA TGGCTTGTCA GGACCTTCGT AAATGGTGGC CTCAGACTAT TCCAGAGAAT 
CTCATTAACA TCAAACAGGA ACACAAGGAA CCTAAAAAGA AAAAGGGTGC TCAAAGTTCT 
CATCTAAAGC AGCCAGCTGG TGGGCCTTTT GGTCCACTGG GCACACCTCT TCCAAAACCC 
AAACCGATTG TTATCAAGAA GCATAAACAA AAAGCTTCCC AGCCAACTTT TCTACCACAG 
ATCCAGATTA CAATATCAAA ACCACCTCTC ATTTCTCCAG GTTATGCACC ATCCCGAGCT 
CAGCTTGTTC CATCTTTGCC AGGCATGGAA GCTGGGCTTC CCCCCACTCA GGTGGCTGAG 
AGCCACCCAG CTCCAGCTCA ATCTCAGGAA TCCATTATAA ATTGCAGTAG TGCACCCATC 
TCTGAAAATA GTCATTCTTC TACTAACCCT GTGTCAGTCC TAGCACCATC AACCCAGAAG 
GTAATAGCAT GCCCAGGCTC AGCTGGATCA AATGACAGCA TCCCAACAAC CAGTATCACA 
CCTCAAACAT CTACAACACA GCCATCTCCT TCTCTGGCTG GCCTCAGTAA CCTAGATCCA 
AAATTTGAAG AGCTGATTCG GCAGTTTGAG GAGGAGTTTG GGGACACTGC AGCTTCTGCA 
GCCGAGACCC CAGAGAATGG CCCTGTTCAA CCAGTGGTGA CCGAGTCGCA CCCAAACTCA 
GGACAATTGA GACCCGAGTC ACCATCCCTA AAGCAGCCAA ACAACTGCCC TCCTGTTCCC 
ACAAATGAGT CTGAGCCCAT GAACGAAGGT CAAAAGGACA TACCTGAAAG ACAGAAGGAA 
AAAACACCAT TGCCACCTGA GGAGCTCTGT ACTGTCAAAC AGGAAAGTGA GGAATGTGCA 
TTGGATGTGA AACCTTCAGG GGAGCTCCTG TCTGAATTTC TGAAGCAACA GAACCCCTTC 
GTTGCACCTT TTTCTCCTCC AAATAAACGT ATGAAGATTG AATCATCCAA TGGTGTGACC 
GTGCTCTCCA CAACTGCATG TTTCTCTGCA GACAGAGAGG GTGATGATTT CAATACTCCC 
ACCAAGGATA TCTTTCCCTC CTCACCTTCA CTTAAAGGTT TTCTTGAGTC ACCATTGCGC 
TACCTGGACA CTCCCACCAA GAACCTGCTT GATACACCTG GGAAAGATGT TCAACCTGAT 
TTTCCAATCT GTGATTGCGT AGACCAAGTC CTTGAAAAGG ATGAAGGTCC ATATTACAAT 
CATTTGGGAT CTGGACGAGA TATACCTTCA GTCAGACAAC TGATGGAAGA TAGGTATGGG 
GAGAAGGGGG AAGCCGTTCG CATTGAAAAG GTGGTTTACA CAGGGCGAGA AGGAAAGAGC 
TCTCAGGGAT GTCCTATTGC TAAGTGGGTG TTACGTCGCA GCAGTGAAAA GGAGAAAGTG 
TTGTGCGTTG TGAAGCAACG GCCTGGGCAC CACTGTGCCA ACACTGTCAT CGTTGTTGTC 
ATCCTGGCCT GGGAAGGTGT GCCCCGTGCT CTGGGAGATA AACTGTACAG AGAAGTCACA 
GAAACCATCA CCAAATATGG CAATCCCACC AGCCGTCGCT GTGGACTCAA TGAAGATCGA 
ACCTGTGCAT GTCAAGGGAA GGACCCAGAG ACCTGTGGAG CTTCATTCTC TTTTGGCTGT 
TCCTGGAGCA TGTATTTCAA TGGATGCAAG TATGCTCGAA GCAAGGTCCC TCGTAAGTTC 
AGACTGCAGG GAGAGCACCC CAAAGAGGAG GACAATCTCA GGGATAACTT CCAAGCTTTG 
GCCACACATG TGGCCCCTCT GTATAAGAAG CTAGCTCCCC AGGCCTACAG CAACCAGTGT 
TTACATGAGG ATGTTGCTTC AGACTGTCGG TTGGGATTGA AGGAAGGACG TCCATTTTCA 
GGAATCACTG CATGCATGGA TTTCTGTGCC CACGCTCACA AAGACCAGCA TAACCTCCAC 
AATGGCTGCA CTGTGGTGTG CACTCTCACG AAAGAGGACA ATCGTACAGT GGGCACAATC 
CCAGAAGATG AGCAGCTGCA CGTGCTTCCG CTCTACAAGC TCGCTACAAC CGACGAGTTT 
GGTAGTGAGG AGAACCAGCG TCTCAAAATG CAGACAGGAG CCATCCAGGT GCTCGCTAAC 
TTCCGTCGAG AGGTTAGAAA ACTTCCAGAA CCTGCAAAAT CATGCCGCCA GCGTCGGTTG 
GAGGCCAAAA AGGCTGCATC AGAGAAAAAG AACAGGAAAT TGCAGCTAGC AGAAACTCCA 
GAGAAGACCG TCAAGATGGA GATTCGCCAT GCAGAGTCAC CTCACATTCA GCAAGGCAAT 
AAAGCTATTC CAAAACAGGA GGTGAAACCA ACAATTAAAA AAGAGCCTGT CGACCACTTC 
CAGCCCTTCA ATGGAGCCAT ACATGGTTAC CAAGCACTAG GGAATGGCAA GACAAACCCT 
GATCCCCATA ACATGAACAG TTCGTTCTCC TACCCTGGGA ACTATGCAAG AGGTCGCATC 
CCTGGCAACA GTCATCCTCC TGCACAGAAC CCCATCAATG GCTTTCACCC TAACCTCCAA 
GATATACGAT ATGGTTATTA CAACTACCCT TCCAGTGCAC TTTTCTCTCC TGAGCTCTTG 
AGATATGATA AAGGAGCCTG GCCCAAAAAC GGAGAACCCT CTGCCCAAGC ATTTGAACAG 
AAGCCGGATG TTCAGTGTCT CCAAGCCAAT CTGGCACAGG CCTATCCCAC CCATCATGGA 
CAGTCTCAGG CACAAATGGC TGATGCATCC ATTCAAGGCT ACCCACAACC CTCTGATATT 
TCGCAGTGTC GTACTCCATC TATGTCTCTT GAGCAGACAC ACCGCTCAAA ACCCATAGTC 
AAGCAGGAGC CCATGGATGT ACCATTGTAT GAAGCAAGAT CAGATGGTCA AGCGCAGAGC 
TGCCCGACCA CTCCTAGTAC AACACCAACA CCTGAAGGAT GGCATGGGCA CAAACCAAAC 
GGCAGTCTAG TACCTAAAGG CTGGGATGGA AACATCAGAG CGGCCTCAAA TAATTCACCC 
TTCACACCAG ATAAGCAGAG GCTCCACCAG CAACACCAAC ACGAACACCC TCAATATCCA 
CAGCAGCAAC AGTGGAATTC CTATTCCTGC CCAAACACTC CAATGGCATC TCCTGCCCCA 
TCTCCGTCCC CATCCCTAAA AGCAGGTCTA TCACCTGCAC CCTCTCCACA CCCGTCCACC 
CCAAGGCAGT GGGGAAGTCC TGCCCCTAGT CCACAACCTA AAGCATGGGG TCCCTATGGT 
CCCATGGGTT ATGGTGCTGG TGTTATCAGG CAAGGCAACC CTGCTGGTGC TTACCCTGAT 
AACATGCTTT CCCAGGCAGG TGTAAATTGT GGCTCAGCAC CCCTTGGACT CCAGGAGAAA 
GCCTGGAAGT CTGGTGGTGG GTCAGCAGCA GGTAGCACGC CATCCCCAGC TCCTGAGGGC 
CGATTGTTTC CCGATGCACT GCAAAGGTCG GACGGCCAGG CATGTTGGGA CAGTGGAGCA 
GAGACACAAA GTGAACGAGA TCCTGAAGAG GACGAGGTTT GGTCGGACAG TGAACACAAC 
TTCTTGGATC CCAACATCGG AGGAGTAGCA GTTGCACCTG CTCATGGTTG TGTCTTGATT 
GAGTGTGCCC GTCGAGAACT CCATGCCACC ACACCGCTTA AAAAGCCTGA CCGCACTCAC 
CCAAGCCGCA TATCCCTGGT CTTCTACCAG CACAAGAACC TGAACCAGCC CTGCCATGGT 
CTGGCTTTGT GGGAATCCAA GATGAAAATC CTGGCAGAGC GAGCGCGACA ACGGCAACAA 
GAAGCTGCTC TTTTGGGTCT CTCACAGGAA GAAATCAAGG CCTACGGGAA AAAGCGCAAA 
TGGGCGGCTG GGTCAGCAAG CCCGTCCCCT GGACAAACTA AGGACAAACG GGAGGGTGTA 
GTGACACGGA TGGCCCCAAC ACAGCATAGC ACCACCATAG TCACAGTTTC ACCTTACCCG 
TCCACACACC TCACTGGTCC TTACAGTCGA TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314874.1
CDS	1226..7384
Translation	MHKLRGGPGPPVQDIYEFSLDEEDPKVFLGARSARVVSPADDYIRSEHEQAGMFSLSQMEDQEPIEPVVAQKKKRKRCGVCGPCMRKENCGTCSNCLNRKIGHQICKLRKCDELKRKKSPWEVEYFEDKNTQPVIPNLAKETVSVVGSVDGPRGKGQMEIGSHERLEEGLLNQELSNGLSKHASNEENVCDNEDRDATKEQHQHNFHEGAGSISQTPGWVLEHTGKLSNPESQQGVWSGQNASTTVNDVDMEDAQTLVAFSVGSLPPYTNHQPSPTPTAQLYERFNQEMSNGGEEAKKKGVDGAFCPPEDLNTLQAALSKARHGHKPPNCDCDGPDCPDYLEWLEKQIKLVTNCQNKTSCNLSGTAQQPLEQHHYSQSQPQQCGGKPLCVQDTNPQPLVNKPSTPRPFGPPAPIPCSPSVLSIAKERNVSLQTAIAIEALTQLSATVPQTVVPPGESSLANHHQHLPTSYHQNMPQVVSSSPGPLLSSSATSAIPQSNHYPQQELTTWEQQRPQSKGEAPTANIFPSSSHYSSSYPSSKPPFTNMHASSPNPRPQQWNQGTASSCEKNSPRNAWMMANTDSKQHFAKPSHESSDPVSELKQLLGDTGGKHPFKFPFPHPSLQDVHKGSFTGMPHIKQEVETKEYLGSECSVMGQEGMLNGQQQFQGQQLSSTIRHSTQAALQQHLHHKRNLFSNSPTYGPLAHMACQDLRKWWPQTIPENLINIKQEHKEPKKKKGAQSSHLKQPAGGPFGPLGTPLPKPKPIVIKKHKQKASQPTFLPQIQITISKPPLISPGYAPSRAQLVPSLPGMEAGLPPTQVAESHPAPAQSQESIINCSSAPISENSHSSTNPVSVLAPSTQKVIACPGSAGSNDSIPTTSITPQTSTTQPSPSLAGLSNLDPKFEELIRQFEEEFGDTAASAAETPENGPVQPVVTESHPNSGQLRPESPSLKQPNNCPPVPTNESEPMNEGQKDIPERQKEKTPLPPEELCTVKQESEECALDVKPSGELLSEFLKQQNPFVAPFSPPNKRMKIESSNGVTVLSTTACFSADREGDDFNTPTKDIFPSSPSLKGFLESPLRYLDTPTKNLLDTPGKDVQPDFPICDCVDQVLEKDEGPYYNHLGSGRDIPSVRQLMEDRYGEKGEAVRIEKVVYTGREGKSSQGCPIAKWVLRRSSEKEKVLCVVKQRPGHHCANTVIVVVILAWEGVPRALGDKLYREVTETITKYGNPTSRRCGLNEDRTCACQGKDPETCGASFSFGCSWSMYFNGCKYARSKVPRKFRLQGEHPKEEDNLRDNFQALATHVAPLYKKLAPQAYSNQCLHEDVASDCRLGLKEGRPFSGITACMDFCAHAHKDQHNLHNGCTVVCTLTKEDNRTVGTIPEDEQLHVLPLYKLATTDEFGSEENQRLKMQTGAIQVLANFRREVRKLPEPAKSCRQRRLEAKKAASEKKNRKLQLAETPEKTVKMEIRHAESPHIQQGNKAIPKQEVKPTIKKEPVDHFQPFNGAIHGYQALGNGKTNPDPHNMNSSFSYPGNYARGRIPGNSHPPAQNPINGFHPNLQDIRYGYYNYPSSALFSPELLRYDKGAWPKNGEPSAQAFEQKPDVQCLQANLAQAYPTHHGQSQAQMADASIQGYPQPSDISQCRTPSMSLEQTHRSKPIVKQEPMDVPLYEARSDGQAQSCPTTPSTTPTPEGWHGHKPNGSLVPKGWDGNIRAASNNSPFTPDKQRLHQQHQHEHPQYPQQQQWNSYSCPNTPMASPAPSPSPSLKAGLSPAPSPHPSTPRQWGSPAPSPQPKAWGPYGPMGYGAGVIRQGNPAGAYPDNMLSQAGVNCGSAPLGLQEKAWKSGGGSAAGSTPSPAPEGRLFPDALQRSDGQACWDSGAETQSERDPEEDEVWSDSEHNFLDPNIGGVAVAPAHGCVLIECARRELHATTPLKKPDRTHPSRISLVFYQHKNLNQPCHGLALWESKMKILAERARQRQQEAALLGLSQEEIKAYGKKRKWAAGSASPSPGQTKDKREGVVTRMAPTQHSTTIVTVSPYPSTHLTGPYSRFV

Target ORF information:

RefSeq Version	NM_001327945.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tet methylcytosine dioxygenase 3 (tet3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001327945.1

ORF Insert Sequence:

ATGCACAAGC TGAGGGGAGG CCCTGGCCCT CCAGTCCAGG ACATTTATGA ATTCTCTCTG 
GACGAGGAGG ACCCTAAAGT TTTCCTAGGG GCCAGGAGCG CAAGGGTGGT GTCGCCTGCG 
GACGACTACA TCAGGTCTGA GCATGAGCAA GCTGGCATGT TCTCCTTGTC GCAGATGGAG 
GACCAGGAAC CAATAGAGCC TGTGGTGGCC CAAAAAAAGA AAAGGAAGAG ATGCGGCGTT 
TGTGGGCCAT GCATGAGGAA GGAGAACTGC GGGACCTGTA GCAATTGCCT AAACCGAAAG 
ATCGGACACC AGATCTGCAA ACTGAGAAAG TGTGATGAGT TGAAGAGGAA GAAGAGCCCG 
TGGGAGGTGG AATATTTTGA AGACAAAAAT ACCCAGCCTG TAATCCCAAA CCTGGCTAAA 
GAGACTGTTT CTGTGGTGGG TTCAGTGGAT GGCCCCAGAG GAAAAGGCCA GATGGAAATT 
GGGTCCCATG AACGCCTGGA AGAAGGCTTA TTAAACCAGG AACTCTCTAA TGGGCTTAGC 
AAACATGCAT CTAATGAAGA AAATGTTTGT GATAATGAAG ACAGAGATGC AACCAAGGAG 
CAACACCAGC ATAACTTTCA TGAGGGGGCT GGAAGTATTT CCCAGACACC AGGCTGGGTC 
CTTGAGCATA CTGGGAAGTT GTCAAATCCT GAATCTCAAC AAGGTGTTTG GAGTGGTCAA 
AATGCCAGCA CAACAGTAAA TGATGTGGAT ATGGAGGATG CACAAACTCT GGTGGCCTTT 
TCTGTTGGCT CACTGCCACC TTACACCAAC CACCAACCTT CACCGACCCC TACAGCTCAG 
CTGTATGAAA GATTCAATCA GGAAATGTCC AATGGAGGTG AGGAGGCCAA AAAGAAAGGT 
GTTGATGGTG CGTTTTGCCC CCCTGAAGAT CTAAACACTC TGCAGGCAGC ACTGAGCAAG 
GCCCGTCATG GCCACAAGCC ACCTAATTGT GATTGTGATG GCCCAGACTG CCCTGATTAC 
CTGGAGTGGC TAGAGAAGCA AATTAAATTG GTGACTAACT GTCAGAATAA AACTTCCTGT 
AATCTTTCGG GTACGGCTCA ACAGCCACTT GAACAGCATC ATTATTCCCA ATCCCAACCT 
CAACAATGTG GAGGTAAACC ATTGTGTGTG CAAGACACAA ATCCACAGCC CCTTGTTAAC 
AAGCCTTCTA CTCCTAGACC CTTTGGCCCA CCAGCACCTA TTCCCTGCTC CCCTAGTGTT 
CTGTCCATAG CCAAGGAAAG AAATGTTAGT TTGCAAACAG CCATTGCAAT TGAGGCTCTG 
ACCCAGTTGT CTGCCACTGT CCCACAAACT GTTGTACCTC CTGGTGAGTC TTCACTTGCA 
AATCACCATC AGCATCTGCC AACCTCCTAC CACCAGAACA TGCCACAAGT TGTTTCTTCA 
TCACCTGGTC CTCTGTTGTC CTCCTCTGCC ACCTCTGCTA TTCCACAGTC AAATCATTAC 
CCTCAACAAG AGCTTACAAC ATGGGAGCAA CAGAGACCTC AGTCTAAGGG TGAGGCCCCT 
ACAGCAAACA TTTTTCCTAG CTCCTCTCAT TATTCTTCCT CATACCCATC TTCCAAGCCC 
CCATTTACAA ATATGCACGC ATCAAGCCCC AATCCTCGAC CTCAGCAGTG GAACCAGGGC 
ACAGCTAGTA GCTGTGAGAA AAACTCCCCT CGCAATGCAT GGATGATGGC AAACACAGAC 
TCTAAACAAC ATTTTGCAAA GCCATCCCAT GAGAGCAGTG ACCCAGTGTC TGAGCTGAAG 
CAACTTCTTG GAGACACCGG TGGAAAGCAC CCGTTCAAGT TCCCATTCCC TCACCCCAGC 
TTACAGGATG TTCATAAGGG CAGCTTTACT GGGATGCCTC ACATAAAGCA GGAGGTGGAA 
ACAAAAGAAT ACCTGGGATC AGAATGCTCA GTCATGGGCC AGGAAGGGAT GCTAAATGGC 
CAGCAGCAGT TTCAGGGTCA GCAGTTATCC TCCACCATTA GGCACTCAAC TCAGGCAGCA 
CTACAGCAGC ACCTTCATCA TAAACGTAAT CTCTTCTCTA ATTCCCCAAC ATATGGTCCA 
TTAGCCCACA TGGCTTGTCA GGACCTTCGT AAATGGTGGC CTCAGACTAT TCCAGAGAAT 
CTCATTAACA TCAAACAGGA ACACAAGGAA CCTAAAAAGA AAAAGGGTGC TCAAAGTTCT 
CATCTAAAGC AGCCAGCTGG TGGGCCTTTT GGTCCACTGG GCACACCTCT TCCAAAACCC 
AAACCGATTG TTATCAAGAA GCATAAACAA AAAGCTTCCC AGCCAACTTT TCTACCACAG 
ATCCAGATTA CAATATCAAA ACCACCTCTC ATTTCTCCAG GTTATGCACC ATCCCGAGCT 
CAGCTTGTTC CATCTTTGCC AGGCATGGAA GCTGGGCTTC CCCCCACTCA GGTGGCTGAG 
AGCCACCCAG CTCCAGCTCA ATCTCAGGAA TCCATTATAA ATTGCAGTAG TGCACCCATC 
TCTGAAAATA GTCATTCTTC TACTAACCCT GTGTCAGTCC TAGCACCATC AACCCAGAAG 
GTAATAGCAT GCCCAGGCTC AGCTGGATCA AATGACAGCA TCCCAACAAC CAGTATCACA 
CCTCAAACAT CTACAACACA GCCATCTCCT TCTCTGGCTG GCCTCAGTAA CCTAGATCCA 
AAATTTGAAG AGCTGATTCG GCAGTTTGAG GAGGAGTTTG GGGACACTGC AGCTTCTGCA 
GCCGAGACCC CAGAGAATGG CCCTGTTCAA CCAGTGGTGA CCGAGTCGCA CCCAAACTCA 
GGACAATTGA GACCCGAGTC ACCATCCCTA AAGCAGCCAA ACAACTGCCC TCCTGTTCCC 
ACAAATGAGT CTGAGCCCAT GAACGAAGGT CAAAAGGACA TACCTGAAAG ACAGAAGGAA 
AAAACACCAT TGCCACCTGA GGAGCTCTGT ACTGTCAAAC AGGAAAGTGA GGAATGTGCA 
TTGGATGTGA AACCTTCAGG GGAGCTCCTG TCTGAATTTC TGAAGCAACA GAACCCCTTC 
GTTGCACCTT TTTCTCCTCC AAATAAACGT ATGAAGATTG AATCATCCAA TGGTGTGACC 
GTGCTCTCCA CAACTGCATG TTTCTCTGCA GACAGAGAGG GTGATGATTT CAATACTCCC 
ACCAAGGATA TCTTTCCCTC CTCACCTTCA CTTAAAGGTT TTCTTGAGTC ACCATTGCGC 
TACCTGGACA CTCCCACCAA GAACCTGCTT GATACACCTG GGAAAGATGT TCAACCTGAT 
TTTCCAATCT GTGATTGCGT AGACCAAGTC CTTGAAAAGG ATGAAGGTCC ATATTACAAT 
CATTTGGGAT CTGGACGAGA TATACCTTCA GTCAGACAAC TGATGGAAGA TAGGTATGGG 
GAGAAGGGGG AAGCCGTTCG CATTGAAAAG GTGGTTTACA CAGGGCGAGA AGGAAAGAGC 
TCTCAGGGAT GTCCTATTGC TAAGTGGGTG TTACGTCGCA GCAGTGAAAA GGAGAAAGTG 
TTGTGCGTTG TGAAGCAACG GCCTGGGCAC CACTGTGCCA ACACTGTCAT CGTTGTTGTC 
ATCCTGGCCT GGGAAGGTGT GCCCCGTGCT CTGGGAGATA AACTGTACAG AGAAGTCACA 
GAAACCATCA CCAAATATGG CAATCCCACC AGCCGTCGCT GTGGACTCAA TGAAGATCGA 
ACCTGTGCAT GTCAAGGGAA GGACCCAGAG ACCTGTGGAG CTTCATTCTC TTTTGGCTGT 
TCCTGGAGCA TGTATTTCAA TGGATGCAAG TATGCTCGAA GCAAGGTCCC TCGTAAGTTC 
AGACTGCAGG GAGAGCACCC CAAAGAGGAG GACAATCTCA GGGATAACTT CCAAGCTTTG 
GCCACACATG TGGCCCCTCT GTATAAGAAG CTAGCTCCCC AGGCCTACAG CAACCAGTGT 
TTACATGAGG ATGTTGCTTC AGACTGTCGG TTGGGATTGA AGGAAGGACG TCCATTTTCA 
GGAATCACTG CATGCATGGA TTTCTGTGCC CACGCTCACA AAGACCAGCA TAACCTCCAC 
AATGGCTGCA CTGTGGTGTG CACTCTCACG AAAGAGGACA ATCGTACAGT GGGCACAATC 
CCAGAAGATG AGCAGCTGCA CGTGCTTCCG CTCTACAAGC TCGCTACAAC CGACGAGTTT 
GGTAGTGAGG AGAACCAGCG TCTCAAAATG CAGACAGGAG CCATCCAGGT GCTCGCTAAC 
TTCCGTCGAG AGGTTAGAAA ACTTCCAGAA CCTGCAAAAT CATGCCGCCA GCGTCGGTTG 
GAGGCCAAAA AGGCTGCATC AGAGAAAAAG AACAGGAAAT TGCAGCTAGC AGAAACTCCA 
GAGAAGACCG TCAAGATGGA GATTCGCCAT GCAGAGTCAC CTCACATTCA GCAAGGCAAT 
AAAGCTATTC CAAAACAGGA GGTGAAACCA ACAATTAAAA AAGAGCCTGT CGACCACTTC 
CAGCCCTTCA ATGGAGCCAT ACATGGTTAC CAAGCACTAG GGAATGGCAA GACAAACCCT 
GATCCCCATA ACATGAACAG TTCGTTCTCC TACCCTGGGA ACTATGCAAG AGGTCGCATC 
CCTGGCAACA GTCATCCTCC TGCACAGAAC CCCATCAATG GCTTTCACCC TAACCTCCAA 
GATATACGAT ATGGTTATTA CAACTACCCT TCCAGTGCAC TTTTCTCTCC TGAGCTCTTG 
AGATATGATA AAGGAGCCTG GCCCAAAAAC GGAGAACCCT CTGCCCAAGC ATTTGAACAG 
AAGCCGGATG TTCAGTGTCT CCAAGCCAAT CTGGCACAGG CCTATCCCAC CCATCATGGA 
CAGTCTCAGG CACAAATGGC TGATGCATCC ATTCAAGGCT ACCCACAACC CTCTGATATT 
TCGCAGTGTC GTACTCCATC TATGTCTCTT GAGCAGACAC ACCGCTCAAA ACCCATAGTC 
AAGCAGGAGC CCATGGATGT ACCATTGTAT GAAGCAAGAT CAGATGGTCA AGCGCAGAGC 
TGCCCGACCA CTCCTAGTAC AACACCAACA CCTGAAGGAT GGCATGGGCA CAAACCAAAC 
GGCAGTCTAG TACCTAAAGG CTGGGATGGA AACATCAGAG CGGCCTCAAA TAATTCACCC 
TTCACACCAG ATAAGCAGAG GCTCCACCAG CAACACCAAC ACGAACACCC TCAATATCCA 
CAGCAGCAAC AGTGGAATTC CTATTCCTGC CCAAACACTC CAATGGCATC TCCTGCCCCA 
TCTCCGTCCC CATCCCTAAA AGCAGGTCTA TCACCTGCAC CCTCTCCACA CCCGTCCACC 
CCAAGGCAGT GGGGAAGTCC TGCCCCTAGT CCACAACCTA AAGCATGGGG TCCCTATGGT 
CCCATGGGTT ATGGTGCTGG TGTTATCAGG CAAGGCAACC CTGCTGGTGC TTACCCTGAT 
AACATGCTTT CCCAGGCAGG TGTAAATTGT GGCTCAGCAC CCCTTGGACT CCAGGAGAAA 
GCCTGGAAGT CTGGTGGTGG GTCAGCAGCA GGTAGCACGC CATCCCCAGC TCCTGAGGGC 
CGATTGTTTC CCGATGCACT GCAAAGGTCG GACGGCCAGG CATGTTGGGA CAGTGGAGCA 
GAGACACAAA GTGAACGAGA TCCTGAAGAG GACGAGGTTT GGTCGGACAG TGAACACAAC 
TTCTTGGATC CCAACATCGG AGGAGTAGCA GTTGCACCTG CTCATGGTTG TGTCTTGATT 
GAGTGTGCCC GTCGAGAACT CCATGCCACC ACACCGCTTA AAAAGCCTGA CCGCACTCAC 
CCAAGCCGCA TATCCCTGGT CTTCTACCAG CACAAGAACC TGAACCAGCC CTGCCATGGT 
CTGGCTTTGT GGGAATCCAA GATGAAAATC CTGGCAGAGC GAGCGCGACA ACGGCAACAA 
GAAGCTGCTC TTTTGGGTCT CTCACAGGAA GAAATCAAGG CCTACGGGAA AAAGCGCAAA 
TGGGCGGCTG GGTCAGCAAG CCCGTCCCCT GGACAAACTA AGGACAAACG GGAGGGTGTA 
GTGACACGGA TGGCCCCAAC ACAGCATAGC ACCACCATAG TCACAGTTTC ACCTTACCCG 
TCCACACACC TCACTGGTCC TTACAGTCGA TTTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tet1 facilitates hypoxia tolerance by stabilizing the HIF-? proteins independent of its methylcytosine dioxygenase activity.
Nucleic acids research45(22)12700-12714(2017 Dec)
Wang J,Zhang D,Du J,Zhou C,Li Z,Liu X,Ouyang G,Xiao W

Tet-mediated DNA hydroxymethylation regulates retinal neurogenesis by modulating cell-extrinsic signaling pathways.
PLoS genetics13(9)e1006987(2017 Sep)
Seritrakul P,Gross JM

Zebrafish akt2 is essential for survival, growth, bone development, and glucose homeostasis.
Mechanisms of development14342-52(2017 Feb)
Zhang D,Wang J,Zhou C,Xiao W

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tet3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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