ano5a cDNA ORF clone, Danio rerio(zebrafish)

The following ano5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ano5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa29684 XM_005163223.3
Latest version!
Danio rerio anoctamin 5a (ano5a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa50064 XM_009298193.3
Latest version!
Danio rerio anoctamin 5a (ano5a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa141505 XM_021470757.1
Latest version!
Danio rerio anoctamin 5a (ano5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa141506 XM_021470758.1
Latest version!
Danio rerio anoctamin 5a (ano5a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa50065 XM_021470760.1
Latest version!
Danio rerio anoctamin 5a (ano5a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
ODa50064 XM_009298193.2 Danio rerio anoctamin 5a (ano5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa29684
    Clone ID Related Accession (Same CDS sequence) XM_005163223.3
    Accession Version XM_005163223.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product anoctamin-5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163223.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATCGCG GATCGCAAAA GTCGAGTGAT GAGACGCTTA TAGAAATGAG CCGAACTGCT 
    TGGTCCAGCG GCGAAGACAA CAATGGCTTC CATTCCAATT CGGATAAGGA GACGTTTCAA
    CTTGAGAATG CTGAGGTGGA TAAACAGCAG CAGAGTAAGG ACTCTGTGTA CTTCAGGGAT
    GGTGTACGGA GGATAGATTT CGTCCTGTCT TACATCGACG ACAAGGATGG AGAAAAGAAA
    CAGGACCGAA GGAAGGTGTT TGAGGCTAAC TTACAGAAAG CCGGACTGGA GGTGGAGACC
    GAGGACAAAT CTGAGTCTGA GGATGGAAAG ACGTACTTCT TGAAGATCCA CGTTCCATGG
    GAGGTGCTGG CGACATATGC CGACGTCCTG AAAATCAAAG TGCCTTTCAA AGCCAACGAT
    ATTCCTGAAA ATAAAGAGAT ACCGATGGGC TGGCTCTTCA CGCCCTTCCG CCTGCCCGAC
    CATGTGATGA ATCCAGAGCC CGACTTCTTC ACCTCCACGT TCGACAAGAG CAAGATCGAC
    TTCTTCCTTA TTGAAGATAA AGAAACCCTC TTTCCTGCGT CCACGCGCAA CAGGATTGTT
    TATTACCTCC TTTCCCGAAT CCCGTACAGC ACAGAAGACA AAGATAAGAA GGGTATTAAG
    AGGTTGCTGA ACAACGGGAC CTACACGTCC GCCTTCCCTC TGCATGACTG TCGATATTGG
    ACCAGGTCTC GTGATGATAA CTGTGAGAGC GAACGCTATT CTCTGTATAA AAACTGGGCT
    CGCTTCAGCA GCTTCTACAA GGAGCAGCCG CTCAACCTCA TTCGGAAGTA CTATGGAGAG
    AAGATCGGCA TCTACTTTGC TTGGCTGGGT TTTTACACGG AGATGCTGTC CTATGCTGCG
    GTTGTGGGTT TACTCTGCTT CATCTATGGA GTGGCCACCT TTGACGAAAA CATCTGGAGT
    CGAGAGATCT GCAACGAGAC CATCGGTGGG CAGATTGTCA TGTGTCCGCT CTGTGATAAG
    AAATGCGGCT ACTGGAAGCT CAGCACTACC TGCAGCTCTT CATGGCAATC TTATTTATTT
    GACAATGCAG CGACGGTGTT CTTTGCTATA TTCATGGGAA TATGGGTCAC TTTATTCCTG
    GAGTTCTGGA AGCGGCGTCA GGCCCGGCTG GAGTACGAGT GGGATCTGGT GGACTTTGAG
    GAAGAGCAAC AGCAGCTCCA GCTCAGACCC GAATACGAGT CCAAGTGCAC CAATCGGCGA
    CTCAACCGCG TCACACAGGA GATGGAGCCT TATTTACCTC TAACAAGCAA GTGTGCTCGT
    TCAATACTGT CTGGGGCCAC CGTCCTCTTC TGGATCTCTC TGATTATCGC CAGTATTATC
    GGTGTGATTG CGTATCGTCT GGCGGTGTTC GCTGCATTCG CGAGCATCAT GAAGGACAGT
    CCCACTAATA AGCTGGAGAT AGTGGGAAAC CTGATCACGC CTCAGTTCGC TACGTCTGTA
    ACGGCGTCCT GCATCAACTT TGTCATCATC ATGGTGCTGA ACTTCCTGTA TGAGCGCGTC
    GCCATCAAGA TCACTGATAT GGAGGTCCCT AAAACACACG TCGAGTACGA GAACAAGCTG
    ACGATAAAGA TGTTCCTCTT CCAGTTCGTC AACTATTATT CCTCCTGCTT CTACGTTGCT
    TTCTTCAAGG GTAAATTTGT GGGATATCCT GGAAACTACG CCTACATGTT TGGGCCTTGG
    AGTGGACTGA GAAATGAAGA GTGTGAGCCT GGCGGCTGTC TGATCGAGCT GACCACTCAG
    TTAGTGATCG TGATGGTGGG CAAGCAGGTG TGGGGGAACA TTCAAGAAGC TCTCGTCCCC
    TGGCTGCGTA ACTGGTGGGT GAGCAGAAGT GCTCGTAATC ATCCGGAGAG TCTGTACAGC
    CGCTGGGAGC AGGATCACGA TCTGCAGAAC TTGGGGCAGC TCGGCCTCTT CTACGAGTAC
    CTGGAGATGG TGATTCAGTT TGGCTTCATC ACTCTGTTCG TGGCCTCTTT TCCTCTGGCT
    CCGCTCCTGG CTCTGATGAA TAATATCCTG GAGGTGCGCG TGGACGCCTG GAAGTTCACC
    ACTCAGTTCC GCAGACCCGT CGCTGCTAAA GCGCACAGCA TCGGCGCCTG GGAGGAAATC
    CTCAACATGA TCGCAGTCTT CTCCGTCGTC ACCAACGCTT TCATCGTGTC CTTCACGTCT
    GACATGATCC CGCGGCTGGT GTACCAGTAC GTGCATAATA CGGGCAATGC GGCCTCTATG
    TCGGGATACA TCACCCACAG CCTCTCCATC TACAACATCT CAGAGTTTCC TCCCGAAAAC
    CGTCCGGATG ATGACGAAAA CCCAGACTGG TTCAACGCCG GCAACTACAC CACCTGCAGG
    TATCGAGATT ATCGATATCC TCCAGGACAC GAGAAGCAGT ACACCCACAA CATGGACTTC
    TGGCACATCC TCGCCGCCAA ACTGGCATTC ATCATCATCA TGGAGCATGT GGTGTTTGTG
    GTGAAGTTTT TCGTGGCGTG GTTGATCCCC GACGTGCCTT CAGACGTAAA AGCGCGTGTA
    AAGCGCGAGC GTTACCTCAT CCAGGAGTAT CTGCACGACT ATGAGGTGGA AAAACTGAAG
    AACCAGCTCA GCCTGAACAC TGACGTCCAC AATGACATTA CGGCATGCAC CTGTCCCACC
    ACAGACGCCA TCCCCAAACT GGAGGTATTA TCAGACTGTC TGACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005163280.1
    CDS145..2892
    Translation

    Target ORF information:

    RefSeq Version XM_005163223.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio anoctamin 5a (ano5a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005163223.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATCGCG GATCGCAAAA GTCGAGTGAT GAGACGCTTA TAGAAATGAG CCGAACTGCT 
    TGGTCCAGCG GCGAAGACAA CAATGGCTTC CATTCCAATT CGGATAAGGA GACGTTTCAA
    CTTGAGAATG CTGAGGTGGA TAAACAGCAG CAGAGTAAGG ACTCTGTGTA CTTCAGGGAT
    GGTGTACGGA GGATAGATTT CGTCCTGTCT TACATCGACG ACAAGGATGG AGAAAAGAAA
    CAGGACCGAA GGAAGGTGTT TGAGGCTAAC TTACAGAAAG CCGGACTGGA GGTGGAGACC
    GAGGACAAAT CTGAGTCTGA GGATGGAAAG ACGTACTTCT TGAAGATCCA CGTTCCATGG
    GAGGTGCTGG CGACATATGC CGACGTCCTG AAAATCAAAG TGCCTTTCAA AGCCAACGAT
    ATTCCTGAAA ATAAAGAGAT ACCGATGGGC TGGCTCTTCA CGCCCTTCCG CCTGCCCGAC
    CATGTGATGA ATCCAGAGCC CGACTTCTTC ACCTCCACGT TCGACAAGAG CAAGATCGAC
    TTCTTCCTTA TTGAAGATAA AGAAACCCTC TTTCCTGCGT CCACGCGCAA CAGGATTGTT
    TATTACCTCC TTTCCCGAAT CCCGTACAGC ACAGAAGACA AAGATAAGAA GGGTATTAAG
    AGGTTGCTGA ACAACGGGAC CTACACGTCC GCCTTCCCTC TGCATGACTG TCGATATTGG
    ACCAGGTCTC GTGATGATAA CTGTGAGAGC GAACGCTATT CTCTGTATAA AAACTGGGCT
    CGCTTCAGCA GCTTCTACAA GGAGCAGCCG CTCAACCTCA TTCGGAAGTA CTATGGAGAG
    AAGATCGGCA TCTACTTTGC TTGGCTGGGT TTTTACACGG AGATGCTGTC CTATGCTGCG
    GTTGTGGGTT TACTCTGCTT CATCTATGGA GTGGCCACCT TTGACGAAAA CATCTGGAGT
    CGAGAGATCT GCAACGAGAC CATCGGTGGG CAGATTGTCA TGTGTCCGCT CTGTGATAAG
    AAATGCGGCT ACTGGAAGCT CAGCACTACC TGCAGCTCTT CATGGCAATC TTATTTATTT
    GACAATGCAG CGACGGTGTT CTTTGCTATA TTCATGGGAA TATGGGTCAC TTTATTCCTG
    GAGTTCTGGA AGCGGCGTCA GGCCCGGCTG GAGTACGAGT GGGATCTGGT GGACTTTGAG
    GAAGAGCAAC AGCAGCTCCA GCTCAGACCC GAATACGAGT CCAAGTGCAC CAATCGGCGA
    CTCAACCGCG TCACACAGGA GATGGAGCCT TATTTACCTC TAACAAGCAA GTGTGCTCGT
    TCAATACTGT CTGGGGCCAC CGTCCTCTTC TGGATCTCTC TGATTATCGC CAGTATTATC
    GGTGTGATTG CGTATCGTCT GGCGGTGTTC GCTGCATTCG CGAGCATCAT GAAGGACAGT
    CCCACTAATA AGCTGGAGAT AGTGGGAAAC CTGATCACGC CTCAGTTCGC TACGTCTGTA
    ACGGCGTCCT GCATCAACTT TGTCATCATC ATGGTGCTGA ACTTCCTGTA TGAGCGCGTC
    GCCATCAAGA TCACTGATAT GGAGGTCCCT AAAACACACG TCGAGTACGA GAACAAGCTG
    ACGATAAAGA TGTTCCTCTT CCAGTTCGTC AACTATTATT CCTCCTGCTT CTACGTTGCT
    TTCTTCAAGG GTAAATTTGT GGGATATCCT GGAAACTACG CCTACATGTT TGGGCCTTGG
    AGTGGACTGA GAAATGAAGA GTGTGAGCCT GGCGGCTGTC TGATCGAGCT GACCACTCAG
    TTAGTGATCG TGATGGTGGG CAAGCAGGTG TGGGGGAACA TTCAAGAAGC TCTCGTCCCC
    TGGCTGCGTA ACTGGTGGGT GAGCAGAAGT GCTCGTAATC ATCCGGAGAG TCTGTACAGC
    CGCTGGGAGC AGGATCACGA TCTGCAGAAC TTGGGGCAGC TCGGCCTCTT CTACGAGTAC
    CTGGAGATGG TGATTCAGTT TGGCTTCATC ACTCTGTTCG TGGCCTCTTT TCCTCTGGCT
    CCGCTCCTGG CTCTGATGAA TAATATCCTG GAGGTGCGCG TGGACGCCTG GAAGTTCACC
    ACTCAGTTCC GCAGACCCGT CGCTGCTAAA GCGCACAGCA TCGGCGCCTG GGAGGAAATC
    CTCAACATGA TCGCAGTCTT CTCCGTCGTC ACCAACGCTT TCATCGTGTC CTTCACGTCT
    GACATGATCC CGCGGCTGGT GTACCAGTAC GTGCATAATA CGGGCAATGC GGCCTCTATG
    TCGGGATACA TCACCCACAG CCTCTCCATC TACAACATCT CAGAGTTTCC TCCCGAAAAC
    CGTCCGGATG ATGACGAAAA CCCAGACTGG TTCAACGCCG GCAACTACAC CACCTGCAGG
    TATCGAGATT ATCGATATCC TCCAGGACAC GAGAAGCAGT ACACCCACAA CATGGACTTC
    TGGCACATCC TCGCCGCCAA ACTGGCATTC ATCATCATCA TGGAGCATGT GGTGTTTGTG
    GTGAAGTTTT TCGTGGCGTG GTTGATCCCC GACGTGCCTT CAGACGTAAA AGCGCGTGTA
    AAGCGCGAGC GTTACCTCAT CCAGGAGTAT CTGCACGACT ATGAGGTGGA AAAACTGAAG
    AACCAGCTCA GCCTGAACAC TGACGTCCAC AATGACATTA CGGCATGCAC CTGTCCCACC
    ACAGACGCCA TCCCCAAACT GGAGGTATTA TCAGACTGTC TGACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50064
    Clone ID Related Accession (Same CDS sequence) XM_009298193.3 , XM_009298193.2
    Accession Version XM_009298193.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product anoctamin-5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATCGCG GATCGCAAAA GTCGAGTGAT GAGACGCTTA TAGAAATGAG CCGAACTGCT 
    TGGTCCAGCG GCGAAGACAA CAATGGCTTC CATTCCAATT CGGATAAGGA GACGTTTCAA
    CTTGAGAATG CTGAGGTGGA TAAACAGCAG CAGAGTAAGG ACTCTGTGTA CTTCAGGGAT
    GGTGTACGGA GGATAGATTT CGTCCTGTCT TACATCGACG ACAAGGATGG AGAAAAGAAA
    CAGGACCGAA GGAAGGTGTT TGAGGCTAAC TTACAGAAAG CCGGACTGGA GGTGGAGACC
    GAGGACAAAT CTGAGTCTGA GGATGGAAAG ACGTACTTCT TGAAGATCCA CGTTCCATGG
    GAGGTGCTGG CGACATATGC CGACGTCCTG AAAATCAAAG TGCCTTTCAA AGCCAACGAT
    ATTCCTGAAA ATAAAGAGAT ACCGATGGGC TGGCTCTTCA CGCCCTTCCG CCTGCCCGAC
    CATGTGATGA ATCCAGAGCC CGACTTCTTC ACCTCCACGT TCGACAAGAG CAAGATCGAC
    TTCTTCCTTA TTGAAGATAA AGAAACCCTC TTTCCTGCGT CCACGCGCAA CAGGATTGTT
    TATTACCTCC TTTCCCGAAT CCCGTACAGC ACAGAAGACA AAGATAAGAA GGGTATTAAG
    AGGTTGCTGA ACAACGGGAC CTACACGTCC GCCTTCCCTC TGCATGACTG TCGATATTGG
    ACCAGGTCTC GTGATGATAA CTGTGAGAGC GAACGCTATT CTCTGTATAA AAACTGGGCT
    CGCTTCAGCA GCTTCTACAA GGAGCAGCCG CTCAACCTCA TTCGGAAGTA CTATGGAGAG
    AAGATCGGCA TCTACTTTGC TTGGCTGGGT TTTTACACGG AGATGCTGTC CTATGCTGCG
    GTTGTGGGTT TACTCTGCTT CATCTATGGA GTGGCCACCT TTGACGAAAA CATCTGGAGT
    CGAGAGATCT GCAACGAGAC CATCGGTGGG CAGATTGTCA TGTGTCCGCT CTGTGATAAG
    AAATGCGGCT ACTGGAAGCT CAGCACTACC TGCAGCTCTT CATGGCAATC TTATTTATTT
    GACAATGCAG CGACGGTGTT CTTTGCTATA TTCATGGGAA TATGGGTCAC TTTATTCCTG
    GAGTTCTGGA AGCGGCGTCA GGCCCGGCTG GAGTACGAGT GGGATCTGGT GGACTTTGAG
    GAAGAGCAAC AGCAGCTCCA GCTCAGACCC GAATACGAGT CCAAGTGCAC CAATCGGCGA
    CTCAACCGCG TCACACAGGA ACAGGAGTGG GTTCTAGACC AGACTCCAGC CGATAAAGCA
    GGCAGGTTTG TCCTGTGCTG GACCACTGTT CTCTTATGGA TCTCTCTGAT TATCGCCAGT
    ATTATCGGTG TGATTGCGTA TCGTCTGGCG GTGTTCGCTG CATTCGCGAG CATCATGAAG
    GACAGTCCCA CTAATAAGCT GGAGATAGTG GGAAACCTGA TCACGCCTCA GTTCGCTACG
    TCTGTAACGG CGTCCTGCAT CAACTTTGTC ATCATCATGG TGCTGAACTT CCTGTATGAG
    CGCGTCGCCA TCAAGATCAC TGATATGGAG GTCCCTAAAA CACACGTCGA GTACGAGAAC
    AAGCTGACGA TAAAGATGTT CCTCTTCCAG TTCGTCAACT ATTATTCCTC CTGCTTCTAC
    GTTGCTTTCT TCAAGGGTAA ATTTGTGGGA TATCCTGGAA ACTACGCCTA CATGTTTGGG
    CCTTGGAGTG GACTGAGAAA TGAAGAGTGT GAGCCTGGCG GCTGTCTGAT CGAGCTGACC
    ACTCAGTTAG TGATCGTGAT GGTGGGCAAG CAGGTGTGGG GGAACATTCA AGAAGCTCTC
    GTCCCCTGGC TGCGTAACTG GTGGGTGAGC AGAAGTGCTC GTAATCATCC GGAGAGTCTG
    TACAGCCGCT GGGAGCAGGA TCACGATCTG CAGAACTTGG GGCAGCTCGG CCTCTTCTAC
    GAGTACCTGG AGATGGTGAT TCAGTTTGGC TTCATCACTC TGTTCGTGGC CTCTTTTCCT
    CTGGCTCCGC TCCTGGCTCT GATGAATAAT ATCCTGGAGG TGCGCGTGGA CGCCTGGAAG
    TTCACCACTC AGTTCCGCAG ACCCGTCGCT GCTAAAGCGC ACAGCATCGG CGCCTGGGAG
    GAAATCCTCA ACATGATCGC AGTCTTCTCC GTCGTCACCA ACGCTTTCAT CGTGTCCTTC
    ACGTCTGACA TGATCCCGCG GCTGGTGTAC CAGTACGTGC ATAATACGGG CAATGCGGCC
    TCTATGTCGG GATACATCAC CCACAGCCTC TCCATCTACA ACATCTCAGA GTTTCCTCCC
    GAAAACCGTC CGGATGATGA CGAAAACCCA GACTGGTTCA ACGCCGGCAA CTACACCACC
    TGCAGGTATC GAGATTATCG ATATCCTCCA GGACACGAGA AGCAGTACAC CCACAACATG
    GACTTCTGGC ACATCCTCGC CGCCAAACTG GCATTCATCA TCATCATGGA GCATGTGGTG
    TTTGTGGTGA AGTTTTTCGT GGCGTGGTTG ATCCCCGACG TGCCTTCAGA CGTAAAAGCG
    CGTGTAAAGC GCGAGCGTTA CCTCATCCAG GAGTATCTGC ACGACTATGA GGTGGAAAAA
    CTGAAGAACC AGCTCAGCCT GAACACTGAC GTCCACAATG ACATTACGGC ATGCACCTGT
    CCCACCACAG ACGCCATCCC CAAACTGGAG GTATTATCAG ACTGTCTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296468.1
    CDS169..2922
    Translation

    Target ORF information:

    RefSeq Version XM_009298193.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 5a (ano5a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298193.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATCGCG GATCGCAAAA GTCGAGTGAT GAGACGCTTA TAGAAATGAG CCGAACTGCT 
    TGGTCCAGCG GCGAAGACAA CAATGGCTTC CATTCCAATT CGGATAAGGA GACGTTTCAA
    CTTGAGAATG CTGAGGTGGA TAAACAGCAG CAGAGTAAGG ACTCTGTGTA CTTCAGGGAT
    GGTGTACGGA GGATAGATTT CGTCCTGTCT TACATCGACG ACAAGGATGG AGAAAAGAAA
    CAGGACCGAA GGAAGGTGTT TGAGGCTAAC TTACAGAAAG CCGGACTGGA GGTGGAGACC
    GAGGACAAAT CTGAGTCTGA GGATGGAAAG ACGTACTTCT TGAAGATCCA CGTTCCATGG
    GAGGTGCTGG CGACATATGC CGACGTCCTG AAAATCAAAG TGCCTTTCAA AGCCAACGAT
    ATTCCTGAAA ATAAAGAGAT ACCGATGGGC TGGCTCTTCA CGCCCTTCCG CCTGCCCGAC
    CATGTGATGA ATCCAGAGCC CGACTTCTTC ACCTCCACGT TCGACAAGAG CAAGATCGAC
    TTCTTCCTTA TTGAAGATAA AGAAACCCTC TTTCCTGCGT CCACGCGCAA CAGGATTGTT
    TATTACCTCC TTTCCCGAAT CCCGTACAGC ACAGAAGACA AAGATAAGAA GGGTATTAAG
    AGGTTGCTGA ACAACGGGAC CTACACGTCC GCCTTCCCTC TGCATGACTG TCGATATTGG
    ACCAGGTCTC GTGATGATAA CTGTGAGAGC GAACGCTATT CTCTGTATAA AAACTGGGCT
    CGCTTCAGCA GCTTCTACAA GGAGCAGCCG CTCAACCTCA TTCGGAAGTA CTATGGAGAG
    AAGATCGGCA TCTACTTTGC TTGGCTGGGT TTTTACACGG AGATGCTGTC CTATGCTGCG
    GTTGTGGGTT TACTCTGCTT CATCTATGGA GTGGCCACCT TTGACGAAAA CATCTGGAGT
    CGAGAGATCT GCAACGAGAC CATCGGTGGG CAGATTGTCA TGTGTCCGCT CTGTGATAAG
    AAATGCGGCT ACTGGAAGCT CAGCACTACC TGCAGCTCTT CATGGCAATC TTATTTATTT
    GACAATGCAG CGACGGTGTT CTTTGCTATA TTCATGGGAA TATGGGTCAC TTTATTCCTG
    GAGTTCTGGA AGCGGCGTCA GGCCCGGCTG GAGTACGAGT GGGATCTGGT GGACTTTGAG
    GAAGAGCAAC AGCAGCTCCA GCTCAGACCC GAATACGAGT CCAAGTGCAC CAATCGGCGA
    CTCAACCGCG TCACACAGGA ACAGGAGTGG GTTCTAGACC AGACTCCAGC CGATAAAGCA
    GGCAGGTTTG TCCTGTGCTG GACCACTGTT CTCTTATGGA TCTCTCTGAT TATCGCCAGT
    ATTATCGGTG TGATTGCGTA TCGTCTGGCG GTGTTCGCTG CATTCGCGAG CATCATGAAG
    GACAGTCCCA CTAATAAGCT GGAGATAGTG GGAAACCTGA TCACGCCTCA GTTCGCTACG
    TCTGTAACGG CGTCCTGCAT CAACTTTGTC ATCATCATGG TGCTGAACTT CCTGTATGAG
    CGCGTCGCCA TCAAGATCAC TGATATGGAG GTCCCTAAAA CACACGTCGA GTACGAGAAC
    AAGCTGACGA TAAAGATGTT CCTCTTCCAG TTCGTCAACT ATTATTCCTC CTGCTTCTAC
    GTTGCTTTCT TCAAGGGTAA ATTTGTGGGA TATCCTGGAA ACTACGCCTA CATGTTTGGG
    CCTTGGAGTG GACTGAGAAA TGAAGAGTGT GAGCCTGGCG GCTGTCTGAT CGAGCTGACC
    ACTCAGTTAG TGATCGTGAT GGTGGGCAAG CAGGTGTGGG GGAACATTCA AGAAGCTCTC
    GTCCCCTGGC TGCGTAACTG GTGGGTGAGC AGAAGTGCTC GTAATCATCC GGAGAGTCTG
    TACAGCCGCT GGGAGCAGGA TCACGATCTG CAGAACTTGG GGCAGCTCGG CCTCTTCTAC
    GAGTACCTGG AGATGGTGAT TCAGTTTGGC TTCATCACTC TGTTCGTGGC CTCTTTTCCT
    CTGGCTCCGC TCCTGGCTCT GATGAATAAT ATCCTGGAGG TGCGCGTGGA CGCCTGGAAG
    TTCACCACTC AGTTCCGCAG ACCCGTCGCT GCTAAAGCGC ACAGCATCGG CGCCTGGGAG
    GAAATCCTCA ACATGATCGC AGTCTTCTCC GTCGTCACCA ACGCTTTCAT CGTGTCCTTC
    ACGTCTGACA TGATCCCGCG GCTGGTGTAC CAGTACGTGC ATAATACGGG CAATGCGGCC
    TCTATGTCGG GATACATCAC CCACAGCCTC TCCATCTACA ACATCTCAGA GTTTCCTCCC
    GAAAACCGTC CGGATGATGA CGAAAACCCA GACTGGTTCA ACGCCGGCAA CTACACCACC
    TGCAGGTATC GAGATTATCG ATATCCTCCA GGACACGAGA AGCAGTACAC CCACAACATG
    GACTTCTGGC ACATCCTCGC CGCCAAACTG GCATTCATCA TCATCATGGA GCATGTGGTG
    TTTGTGGTGA AGTTTTTCGT GGCGTGGTTG ATCCCCGACG TGCCTTCAGA CGTAAAAGCG
    CGTGTAAAGC GCGAGCGTTA CCTCATCCAG GAGTATCTGC ACGACTATGA GGTGGAAAAA
    CTGAAGAACC AGCTCAGCCT GAACACTGAC GTCCACAATG ACATTACGGC ATGCACCTGT
    CCCACCACAG ACGCCATCCC CAAACTGGAG GTATTATCAG ACTGTCTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50064
    Clone ID Related Accession (Same CDS sequence) XM_009298193.3 , XM_009298193.2
    Accession Version XM_009298193.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product anoctamin-5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATCGCG GATCGCAAAA GTCGAGTGAT GAGACGCTTA TAGAAATGAG CCGAACTGCT 
    TGGTCCAGCG GCGAAGACAA CAATGGCTTC CATTCCAATT CGGATAAGGA GACGTTTCAA
    CTTGAGAATG CTGAGGTGGA TAAACAGCAG CAGAGTAAGG ACTCTGTGTA CTTCAGGGAT
    GGTGTACGGA GGATAGATTT CGTCCTGTCT TACATCGACG ACAAGGATGG AGAAAAGAAA
    CAGGACCGAA GGAAGGTGTT TGAGGCTAAC TTACAGAAAG CCGGACTGGA GGTGGAGACC
    GAGGACAAAT CTGAGTCTGA GGATGGAAAG ACGTACTTCT TGAAGATCCA CGTTCCATGG
    GAGGTGCTGG CGACATATGC CGACGTCCTG AAAATCAAAG TGCCTTTCAA AGCCAACGAT
    ATTCCTGAAA ATAAAGAGAT ACCGATGGGC TGGCTCTTCA CGCCCTTCCG CCTGCCCGAC
    CATGTGATGA ATCCAGAGCC CGACTTCTTC ACCTCCACGT TCGACAAGAG CAAGATCGAC
    TTCTTCCTTA TTGAAGATAA AGAAACCCTC TTTCCTGCGT CCACGCGCAA CAGGATTGTT
    TATTACCTCC TTTCCCGAAT CCCGTACAGC ACAGAAGACA AAGATAAGAA GGGTATTAAG
    AGGTTGCTGA ACAACGGGAC CTACACGTCC GCCTTCCCTC TGCATGACTG TCGATATTGG
    ACCAGGTCTC GTGATGATAA CTGTGAGAGC GAACGCTATT CTCTGTATAA AAACTGGGCT
    CGCTTCAGCA GCTTCTACAA GGAGCAGCCG CTCAACCTCA TTCGGAAGTA CTATGGAGAG
    AAGATCGGCA TCTACTTTGC TTGGCTGGGT TTTTACACGG AGATGCTGTC CTATGCTGCG
    GTTGTGGGTT TACTCTGCTT CATCTATGGA GTGGCCACCT TTGACGAAAA CATCTGGAGT
    CGAGAGATCT GCAACGAGAC CATCGGTGGG CAGATTGTCA TGTGTCCGCT CTGTGATAAG
    AAATGCGGCT ACTGGAAGCT CAGCACTACC TGCAGCTCTT CATGGCAATC TTATTTATTT
    GACAATGCAG CGACGGTGTT CTTTGCTATA TTCATGGGAA TATGGGTCAC TTTATTCCTG
    GAGTTCTGGA AGCGGCGTCA GGCCCGGCTG GAGTACGAGT GGGATCTGGT GGACTTTGAG
    GAAGAGCAAC AGCAGCTCCA GCTCAGACCC GAATACGAGT CCAAGTGCAC CAATCGGCGA
    CTCAACCGCG TCACACAGGA ACAGGAGTGG GTTCTAGACC AGACTCCAGC CGATAAAGCA
    GGCAGGTTTG TCCTGTGCTG GACCACTGTT CTCTTATGGA TCTCTCTGAT TATCGCCAGT
    ATTATCGGTG TGATTGCGTA TCGTCTGGCG GTGTTCGCTG CATTCGCGAG CATCATGAAG
    GACAGTCCCA CTAATAAGCT GGAGATAGTG GGAAACCTGA TCACGCCTCA GTTCGCTACG
    TCTGTAACGG CGTCCTGCAT CAACTTTGTC ATCATCATGG TGCTGAACTT CCTGTATGAG
    CGCGTCGCCA TCAAGATCAC TGATATGGAG GTCCCTAAAA CACACGTCGA GTACGAGAAC
    AAGCTGACGA TAAAGATGTT CCTCTTCCAG TTCGTCAACT ATTATTCCTC CTGCTTCTAC
    GTTGCTTTCT TCAAGGGTAA ATTTGTGGGA TATCCTGGAA ACTACGCCTA CATGTTTGGG
    CCTTGGAGTG GACTGAGAAA TGAAGAGTGT GAGCCTGGCG GCTGTCTGAT CGAGCTGACC
    ACTCAGTTAG TGATCGTGAT GGTGGGCAAG CAGGTGTGGG GGAACATTCA AGAAGCTCTC
    GTCCCCTGGC TGCGTAACTG GTGGGTGAGC AGAAGTGCTC GTAATCATCC GGAGAGTCTG
    TACAGCCGCT GGGAGCAGGA TCACGATCTG CAGAACTTGG GGCAGCTCGG CCTCTTCTAC
    GAGTACCTGG AGATGGTGAT TCAGTTTGGC TTCATCACTC TGTTCGTGGC CTCTTTTCCT
    CTGGCTCCGC TCCTGGCTCT GATGAATAAT ATCCTGGAGG TGCGCGTGGA CGCCTGGAAG
    TTCACCACTC AGTTCCGCAG ACCCGTCGCT GCTAAAGCGC ACAGCATCGG CGCCTGGGAG
    GAAATCCTCA ACATGATCGC AGTCTTCTCC GTCGTCACCA ACGCTTTCAT CGTGTCCTTC
    ACGTCTGACA TGATCCCGCG GCTGGTGTAC CAGTACGTGC ATAATACGGG CAATGCGGCC
    TCTATGTCGG GATACATCAC CCACAGCCTC TCCATCTACA ACATCTCAGA GTTTCCTCCC
    GAAAACCGTC CGGATGATGA CGAAAACCCA GACTGGTTCA ACGCCGGCAA CTACACCACC
    TGCAGGTATC GAGATTATCG ATATCCTCCA GGACACGAGA AGCAGTACAC CCACAACATG
    GACTTCTGGC ACATCCTCGC CGCCAAACTG GCATTCATCA TCATCATGGA GCATGTGGTG
    TTTGTGGTGA AGTTTTTCGT GGCGTGGTTG ATCCCCGACG TGCCTTCAGA CGTAAAAGCG
    CGTGTAAAGC GCGAGCGTTA CCTCATCCAG GAGTATCTGC ACGACTATGA GGTGGAAAAA
    CTGAAGAACC AGCTCAGCCT GAACACTGAC GTCCACAATG ACATTACGGC ATGCACCTGT
    CCCACCACAG ACGCCATCCC CAAACTGGAG GTATTATCAG ACTGTCTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296468.1
    CDS145..2898
    Translation

    Target ORF information:

    RefSeq Version XM_009298193.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio anoctamin 5a (ano5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009298193.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATCGCG GATCGCAAAA GTCGAGTGAT GAGACGCTTA TAGAAATGAG CCGAACTGCT 
    TGGTCCAGCG GCGAAGACAA CAATGGCTTC CATTCCAATT CGGATAAGGA GACGTTTCAA
    CTTGAGAATG CTGAGGTGGA TAAACAGCAG CAGAGTAAGG ACTCTGTGTA CTTCAGGGAT
    GGTGTACGGA GGATAGATTT CGTCCTGTCT TACATCGACG ACAAGGATGG AGAAAAGAAA
    CAGGACCGAA GGAAGGTGTT TGAGGCTAAC TTACAGAAAG CCGGACTGGA GGTGGAGACC
    GAGGACAAAT CTGAGTCTGA GGATGGAAAG ACGTACTTCT TGAAGATCCA CGTTCCATGG
    GAGGTGCTGG CGACATATGC CGACGTCCTG AAAATCAAAG TGCCTTTCAA AGCCAACGAT
    ATTCCTGAAA ATAAAGAGAT ACCGATGGGC TGGCTCTTCA CGCCCTTCCG CCTGCCCGAC
    CATGTGATGA ATCCAGAGCC CGACTTCTTC ACCTCCACGT TCGACAAGAG CAAGATCGAC
    TTCTTCCTTA TTGAAGATAA AGAAACCCTC TTTCCTGCGT CCACGCGCAA CAGGATTGTT
    TATTACCTCC TTTCCCGAAT CCCGTACAGC ACAGAAGACA AAGATAAGAA GGGTATTAAG
    AGGTTGCTGA ACAACGGGAC CTACACGTCC GCCTTCCCTC TGCATGACTG TCGATATTGG
    ACCAGGTCTC GTGATGATAA CTGTGAGAGC GAACGCTATT CTCTGTATAA AAACTGGGCT
    CGCTTCAGCA GCTTCTACAA GGAGCAGCCG CTCAACCTCA TTCGGAAGTA CTATGGAGAG
    AAGATCGGCA TCTACTTTGC TTGGCTGGGT TTTTACACGG AGATGCTGTC CTATGCTGCG
    GTTGTGGGTT TACTCTGCTT CATCTATGGA GTGGCCACCT TTGACGAAAA CATCTGGAGT
    CGAGAGATCT GCAACGAGAC CATCGGTGGG CAGATTGTCA TGTGTCCGCT CTGTGATAAG
    AAATGCGGCT ACTGGAAGCT CAGCACTACC TGCAGCTCTT CATGGCAATC TTATTTATTT
    GACAATGCAG CGACGGTGTT CTTTGCTATA TTCATGGGAA TATGGGTCAC TTTATTCCTG
    GAGTTCTGGA AGCGGCGTCA GGCCCGGCTG GAGTACGAGT GGGATCTGGT GGACTTTGAG
    GAAGAGCAAC AGCAGCTCCA GCTCAGACCC GAATACGAGT CCAAGTGCAC CAATCGGCGA
    CTCAACCGCG TCACACAGGA ACAGGAGTGG GTTCTAGACC AGACTCCAGC CGATAAAGCA
    GGCAGGTTTG TCCTGTGCTG GACCACTGTT CTCTTATGGA TCTCTCTGAT TATCGCCAGT
    ATTATCGGTG TGATTGCGTA TCGTCTGGCG GTGTTCGCTG CATTCGCGAG CATCATGAAG
    GACAGTCCCA CTAATAAGCT GGAGATAGTG GGAAACCTGA TCACGCCTCA GTTCGCTACG
    TCTGTAACGG CGTCCTGCAT CAACTTTGTC ATCATCATGG TGCTGAACTT CCTGTATGAG
    CGCGTCGCCA TCAAGATCAC TGATATGGAG GTCCCTAAAA CACACGTCGA GTACGAGAAC
    AAGCTGACGA TAAAGATGTT CCTCTTCCAG TTCGTCAACT ATTATTCCTC CTGCTTCTAC
    GTTGCTTTCT TCAAGGGTAA ATTTGTGGGA TATCCTGGAA ACTACGCCTA CATGTTTGGG
    CCTTGGAGTG GACTGAGAAA TGAAGAGTGT GAGCCTGGCG GCTGTCTGAT CGAGCTGACC
    ACTCAGTTAG TGATCGTGAT GGTGGGCAAG CAGGTGTGGG GGAACATTCA AGAAGCTCTC
    GTCCCCTGGC TGCGTAACTG GTGGGTGAGC AGAAGTGCTC GTAATCATCC GGAGAGTCTG
    TACAGCCGCT GGGAGCAGGA TCACGATCTG CAGAACTTGG GGCAGCTCGG CCTCTTCTAC
    GAGTACCTGG AGATGGTGAT TCAGTTTGGC TTCATCACTC TGTTCGTGGC CTCTTTTCCT
    CTGGCTCCGC TCCTGGCTCT GATGAATAAT ATCCTGGAGG TGCGCGTGGA CGCCTGGAAG
    TTCACCACTC AGTTCCGCAG ACCCGTCGCT GCTAAAGCGC ACAGCATCGG CGCCTGGGAG
    GAAATCCTCA ACATGATCGC AGTCTTCTCC GTCGTCACCA ACGCTTTCAT CGTGTCCTTC
    ACGTCTGACA TGATCCCGCG GCTGGTGTAC CAGTACGTGC ATAATACGGG CAATGCGGCC
    TCTATGTCGG GATACATCAC CCACAGCCTC TCCATCTACA ACATCTCAGA GTTTCCTCCC
    GAAAACCGTC CGGATGATGA CGAAAACCCA GACTGGTTCA ACGCCGGCAA CTACACCACC
    TGCAGGTATC GAGATTATCG ATATCCTCCA GGACACGAGA AGCAGTACAC CCACAACATG
    GACTTCTGGC ACATCCTCGC CGCCAAACTG GCATTCATCA TCATCATGGA GCATGTGGTG
    TTTGTGGTGA AGTTTTTCGT GGCGTGGTTG ATCCCCGACG TGCCTTCAGA CGTAAAAGCG
    CGTGTAAAGC GCGAGCGTTA CCTCATCCAG GAGTATCTGC ACGACTATGA GGTGGAAAAA
    CTGAAGAACC AGCTCAGCCT GAACACTGAC GTCCACAATG ACATTACGGC ATGCACCTGT
    CCCACCACAG ACGCCATCCC CAAACTGGAG GTATTATCAG ACTGTCTGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141505
    Clone ID Related Accession (Same CDS sequence) XM_021470757.1
    Accession Version XM_021470757.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product anoctamin-5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATCGCG GATCGCAAAA GTCGAGTGAT GAGACGCTTA TAGAAATGAG CCGAACTGCT 
    TGGTCCAGCG GCGAAGACAA CAATGGCTTC CATTCCAATT CGGATAAGGA GACGTTTCAA
    CTTGAGAATG CTGAGATCTG GAGAAAGAGA GCTTTATTTC TGAAGTATCG CTCCAGGGTG
    GATAAACAGC AGCAGAGTAA GGACTCTGTG TACTTCAGGG ATGGTGTACG GAGGATAGAT
    TTCGTCCTGT CTTACATCGA CGACAAGGAT GGAGAAAAGA AACAGGACCG AAGGAAGGTG
    TTTGAGGCTA ACTTACAGAA AGCCGGACTG GAGGTGGAGA CCGAGGACAA ATCTGAGTCT
    GAGGATGGAA AGACGTACTT CTTGAAGATC CACGTTCCAT GGGAGGTGCT GGCGACATAT
    GCCGACGTCC TGAAAATCAA AGTGCCTTTC AAAGCCAACG ATATTCCTGA AAATAAAGAG
    ATACCGATGG GCTGGCTCTT CACGCCCTTC CGCCTGCCCG ACCATGTGAT GAATCCAGAG
    CCCGACTTCT TCACCTCCAC GTTCGACAAG AGCAAGATCG ACTTCTTCCT TATTGAAGAT
    AAAGAAACCC TCTTTCCTGC GTCCACGCGC AACAGGATTG TTTATTACCT CCTTTCCCGA
    ATCCCGTACA GCACAGAAGA CAAAGATAAG AAGGGTATTA AGAGGTTGCT GAACAACGGG
    ACCTACACGT CCGCCTTCCC TCTGCATGAC TGTCGATATT GGACCAGGTC TCGTGATGAT
    AACTGTGAGA GCGAACGCTA TTCTCTGTAT AAAAACTGGG CTCGCTTCAG CAGCTTCTAC
    AAGGAGCAGC CGCTCAACCT CATTCGGAAG TACTATGGAG AGAAGATCGG CATCTACTTT
    GCTTGGCTGG GTTTTTACAC GGAGATGCTG TCCTATGCTG CGGTTGTGGG TTTACTCTGC
    TTCATCTATG GAGTGGCCAC CTTTGACGAA AACATCTGGA GTCGAGAGAT CTGCAACGAG
    ACCATCGGTG GGCAGATTGT CATGTGTCCG CTCTGTGATA AGAAATGCGG CTACTGGAAG
    CTCAGCACTA CCTGCAGCTC TTCATGGCAA TCTTATTTAT TTGACAATGC AGCGACGGTG
    TTCTTTGCTA TATTCATGGG AATATGGGTC ACTTTATTCC TGGAGTTCTG GAAGCGGCGT
    CAGGCCCGGC TGGAGTACGA GTGGGATCTG GTGGACTTTG AGGAAGAGCA ACAGCAGCTC
    CAGCTCAGAC CCGAATACGA GTCCAAGTGC ACCAATCGGC GACTCAACCG CGTCACACAG
    GAACAGGAGT GGGTTCTAGA CCAGACTCCA GCCGATAAAG CAGGCAGGTT TGTCCTGTGC
    TGGACCACTG TTCTCTTATG GATCTCTCTG ATTATCGCCA GTATTATCGG TGTGATTGCG
    TATCGTCTGG CGGTGTTCGC TGCATTCGCG AGCATCATGA AGGACAGTCC CACTAATAAG
    CTGGAGATAG TGGGAAACCT GATCACGCCT CAGTTCGCTA CGTCTGTAAC GGCGTCCTGC
    ATCAACTTTG TCATCATCAT GGTGCTGAAC TTCCTGTATG AGCGCGTCGC CATCAAGATC
    ACTGATATGG AGGTCCCTAA AACACACGTC GAGTACGAGA ACAAGCTGAC GATAAAGATG
    TTCCTCTTCC AGTTCGTCAA CTATTATTCC TCCTGCTTCT ACGTTGCTTT CTTCAAGGGT
    AAATTTGTGG GATATCCTGG AAACTACGCC TACATGTTTG GGCCTTGGAG TGGACTGAGA
    AATGAAGAGT GTGAGCCTGG CGGCTGTCTG ATCGAGCTGA CCACTCAGTT AGTGATCGTG
    ATGGTGGGCA AGCAGGTGTG GGGGAACATT CAAGAAGCTC TCGTCCCCTG GCTGCGTAAC
    TGGTGGGTGA GCAGAAGTGC TCGTAATCAT CCGGAGAGTC TGTACAGCCG CTGGGAGCAG
    GATCACGATC TGCAGAACTT GGGGCAGCTC GGCCTCTTCT ACGAGTACCT GGAGATGGTG
    ATTCAGTTTG GCTTCATCAC TCTGTTCGTG GCCTCTTTTC CTCTGGCTCC GCTCCTGGCT
    CTGATGAATA ATATCCTGGA GGTGCGCGTG GACGCCTGGA AGTTCACCAC TCAGTTCCGC
    AGACCCGTCG CTGCTAAAGC GCACAGCATC GGCGCCTGGG AGGAAATCCT CAACATGATC
    GCAGTCTTCT CCGTCGTCAC CAACGCTTTC ATCGTGTCCT TCACGTCTGA CATGATCCCG
    CGGCTGGTGT ACCAGTACGT GCATAATACG GGCAATGCGG CCTCTATGTC GGGATACATC
    ACCCACAGCC TCTCCATCTA CAACATCTCA GAGTTTCCTC CCGAAAACCG TCCGGATGAT
    GACGAAAACC CAGACTGGTT CAACGCCGGC AACTACACCA CCTGCAGGTA TCGAGATTAT
    CGATATCCTC CAGGACACGA GAAGCAGTAC ACCCACAACA TGGACTTCTG GCACATCCTC
    GCCGCCAAAC TGGCATTCAT CATCATCATG GAGCATGTGG TGTTTGTGGT GAAGTTTTTC
    GTGGCGTGGT TGATCCCCGA CGTGCCTTCA GACGTAAAAG CGCGTGTAAA GCGCGAGCGT
    TACCTCATCC AGGAGTATCT GCACGACTAT GAGGTGGAAA AACTGAAGAA CCAGCTCAGC
    CTGAACACTG ACGTCCACAA TGACATTACG GCATGCACCT GTCCCACCAC AGACGCCATC
    CCCAAACTGG AGGTATTATC AGACTGTCTG ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326432.1
    CDS169..2964
    Translation

    Target ORF information:

    RefSeq Version XM_021470757.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 5a (ano5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATCGCG GATCGCAAAA GTCGAGTGAT GAGACGCTTA TAGAAATGAG CCGAACTGCT 
    TGGTCCAGCG GCGAAGACAA CAATGGCTTC CATTCCAATT CGGATAAGGA GACGTTTCAA
    CTTGAGAATG CTGAGATCTG GAGAAAGAGA GCTTTATTTC TGAAGTATCG CTCCAGGGTG
    GATAAACAGC AGCAGAGTAA GGACTCTGTG TACTTCAGGG ATGGTGTACG GAGGATAGAT
    TTCGTCCTGT CTTACATCGA CGACAAGGAT GGAGAAAAGA AACAGGACCG AAGGAAGGTG
    TTTGAGGCTA ACTTACAGAA AGCCGGACTG GAGGTGGAGA CCGAGGACAA ATCTGAGTCT
    GAGGATGGAA AGACGTACTT CTTGAAGATC CACGTTCCAT GGGAGGTGCT GGCGACATAT
    GCCGACGTCC TGAAAATCAA AGTGCCTTTC AAAGCCAACG ATATTCCTGA AAATAAAGAG
    ATACCGATGG GCTGGCTCTT CACGCCCTTC CGCCTGCCCG ACCATGTGAT GAATCCAGAG
    CCCGACTTCT TCACCTCCAC GTTCGACAAG AGCAAGATCG ACTTCTTCCT TATTGAAGAT
    AAAGAAACCC TCTTTCCTGC GTCCACGCGC AACAGGATTG TTTATTACCT CCTTTCCCGA
    ATCCCGTACA GCACAGAAGA CAAAGATAAG AAGGGTATTA AGAGGTTGCT GAACAACGGG
    ACCTACACGT CCGCCTTCCC TCTGCATGAC TGTCGATATT GGACCAGGTC TCGTGATGAT
    AACTGTGAGA GCGAACGCTA TTCTCTGTAT AAAAACTGGG CTCGCTTCAG CAGCTTCTAC
    AAGGAGCAGC CGCTCAACCT CATTCGGAAG TACTATGGAG AGAAGATCGG CATCTACTTT
    GCTTGGCTGG GTTTTTACAC GGAGATGCTG TCCTATGCTG CGGTTGTGGG TTTACTCTGC
    TTCATCTATG GAGTGGCCAC CTTTGACGAA AACATCTGGA GTCGAGAGAT CTGCAACGAG
    ACCATCGGTG GGCAGATTGT CATGTGTCCG CTCTGTGATA AGAAATGCGG CTACTGGAAG
    CTCAGCACTA CCTGCAGCTC TTCATGGCAA TCTTATTTAT TTGACAATGC AGCGACGGTG
    TTCTTTGCTA TATTCATGGG AATATGGGTC ACTTTATTCC TGGAGTTCTG GAAGCGGCGT
    CAGGCCCGGC TGGAGTACGA GTGGGATCTG GTGGACTTTG AGGAAGAGCA ACAGCAGCTC
    CAGCTCAGAC CCGAATACGA GTCCAAGTGC ACCAATCGGC GACTCAACCG CGTCACACAG
    GAACAGGAGT GGGTTCTAGA CCAGACTCCA GCCGATAAAG CAGGCAGGTT TGTCCTGTGC
    TGGACCACTG TTCTCTTATG GATCTCTCTG ATTATCGCCA GTATTATCGG TGTGATTGCG
    TATCGTCTGG CGGTGTTCGC TGCATTCGCG AGCATCATGA AGGACAGTCC CACTAATAAG
    CTGGAGATAG TGGGAAACCT GATCACGCCT CAGTTCGCTA CGTCTGTAAC GGCGTCCTGC
    ATCAACTTTG TCATCATCAT GGTGCTGAAC TTCCTGTATG AGCGCGTCGC CATCAAGATC
    ACTGATATGG AGGTCCCTAA AACACACGTC GAGTACGAGA ACAAGCTGAC GATAAAGATG
    TTCCTCTTCC AGTTCGTCAA CTATTATTCC TCCTGCTTCT ACGTTGCTTT CTTCAAGGGT
    AAATTTGTGG GATATCCTGG AAACTACGCC TACATGTTTG GGCCTTGGAG TGGACTGAGA
    AATGAAGAGT GTGAGCCTGG CGGCTGTCTG ATCGAGCTGA CCACTCAGTT AGTGATCGTG
    ATGGTGGGCA AGCAGGTGTG GGGGAACATT CAAGAAGCTC TCGTCCCCTG GCTGCGTAAC
    TGGTGGGTGA GCAGAAGTGC TCGTAATCAT CCGGAGAGTC TGTACAGCCG CTGGGAGCAG
    GATCACGATC TGCAGAACTT GGGGCAGCTC GGCCTCTTCT ACGAGTACCT GGAGATGGTG
    ATTCAGTTTG GCTTCATCAC TCTGTTCGTG GCCTCTTTTC CTCTGGCTCC GCTCCTGGCT
    CTGATGAATA ATATCCTGGA GGTGCGCGTG GACGCCTGGA AGTTCACCAC TCAGTTCCGC
    AGACCCGTCG CTGCTAAAGC GCACAGCATC GGCGCCTGGG AGGAAATCCT CAACATGATC
    GCAGTCTTCT CCGTCGTCAC CAACGCTTTC ATCGTGTCCT TCACGTCTGA CATGATCCCG
    CGGCTGGTGT ACCAGTACGT GCATAATACG GGCAATGCGG CCTCTATGTC GGGATACATC
    ACCCACAGCC TCTCCATCTA CAACATCTCA GAGTTTCCTC CCGAAAACCG TCCGGATGAT
    GACGAAAACC CAGACTGGTT CAACGCCGGC AACTACACCA CCTGCAGGTA TCGAGATTAT
    CGATATCCTC CAGGACACGA GAAGCAGTAC ACCCACAACA TGGACTTCTG GCACATCCTC
    GCCGCCAAAC TGGCATTCAT CATCATCATG GAGCATGTGG TGTTTGTGGT GAAGTTTTTC
    GTGGCGTGGT TGATCCCCGA CGTGCCTTCA GACGTAAAAG CGCGTGTAAA GCGCGAGCGT
    TACCTCATCC AGGAGTATCT GCACGACTAT GAGGTGGAAA AACTGAAGAA CCAGCTCAGC
    CTGAACACTG ACGTCCACAA TGACATTACG GCATGCACCT GTCCCACCAC AGACGCCATC
    CCCAAACTGG AGGTATTATC AGACTGTCTG ACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141506
    Clone ID Related Accession (Same CDS sequence) XM_021470758.1
    Accession Version XM_021470758.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product anoctamin-5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATCGCG GATCGCAAAA GTCGAGTGAT GAGACGCTTA TAGAAATGAG CCGAACTGCT 
    TGGTCCAGCG GCGAAGACAA CAATGGCTTC CATTCCAATT CGGATAAGGA GACGTTTCAA
    CTTGAGAATG CTGAGATCTG GAGAAAGAGA GCTTTATTTC TGAAGTATCG CTCCAGGGTG
    GATAAACAGC AGCAGAGTAA GGACTCTGTG TACTTCAGGG ATGGTGTACG GAGGATAGAT
    TTCGTCCTGT CTTACATCGA CGACAAGGAT GGAGAAAAGA AACAGGACCG AAGGAAGGTG
    TTTGAGGCTA ACTTACAGAA AGCCGGACTG GAGGTGGAGA CCGAGGACAA ATCTGAGTCT
    GAGGATGGAA AGACGTACTT CTTGAAGATC CACGTTCCAT GGGAGGTGCT GGCGACATAT
    GCCGACGTCC TGAAAATCAA AGTGCCTTTC AAAGCCAACG ATATTCCTGA AAATAAAGAG
    ATACCGATGG GCTGGCTCTT CACGCCCTTC CGCCTGCCCG ACCATGTGAT GAATCCAGAG
    CCCGACTTCT TCACCTCCAC GTTCGACAAG AGCAAGATCG ACTTCTTCCT TATTGAAGAT
    AAAGAAACCC TCTTTCCTGC GTCCACGCGC AACAGGATTG TTTATTACCT CCTTTCCCGA
    ATCCCGTACA GCACAGAAGA CAAAGATAAG AAGGGTATTA AGAGGTTGCT GAACAACGGG
    ACCTACACGT CCGCCTTCCC TCTGCATGAC TGTCGATATT GGACCAGGTC TCGTGATGAT
    AACTGTGAGA GCGAACGCTA TTCTCTGTAT AAAAACTGGG CTCGCTTCAG CAGCTTCTAC
    AAGGAGCAGC CGCTCAACCT CATTCGGAAG TACTATGGAG AGAAGATCGG CATCTACTTT
    GCTTGGCTGG GTTTTTACAC GGAGATGCTG TCCTATGCTG CGGTTGTGGG TTTACTCTGC
    TTCATCTATG GAGTGGCCAC CTTTGACGAA AACATCTGGA GTCGAGAGAT CTGCAACGAG
    ACCATCGGTG GGCAGATTGT CATGTGTCCG CTCTGTGATA AGAAATGCGG CTACTGGAAG
    CTCAGCACTA CCTGCAGCTC TTCATGGCAA TCTTATTTAT TTGACAATGC AGCGACGGTG
    TTCTTTGCTA TATTCATGGG AATATGGGTC ACTTTATTCC TGGAGTTCTG GAAGCGGCGT
    CAGGCCCGGC TGGAGTACGA GTGGGATCTG GTGGACTTTG AGGAAGAGCA ACAGCAGCTC
    CAGCTCAGAC CCGAATACGA GTCCAAGTGC ACCAATCGGC GACTCAACCG CGTCACACAG
    GAGATGGAGC CTTATTTACC TCTAACAAGC AAGTGTGCTC GTTCAATACT GTCTGGGGCC
    ACCGTCCTCT TCTGGATCTC TCTGATTATC GCCAGTATTA TCGGTGTGAT TGCGTATCGT
    CTGGCGGTGT TCGCTGCATT CGCGAGCATC ATGAAGGACA GTCCCACTAA TAAGCTGGAG
    ATAGTGGGAA ACCTGATCAC GCCTCAGTTC GCTACGTCTG TAACGGCGTC CTGCATCAAC
    TTTGTCATCA TCATGGTGCT GAACTTCCTG TATGAGCGCG TCGCCATCAA GATCACTGAT
    ATGGAGGTCC CTAAAACACA CGTCGAGTAC GAGAACAAGC TGACGATAAA GATGTTCCTC
    TTCCAGTTCG TCAACTATTA TTCCTCCTGC TTCTACGTTG CTTTCTTCAA GGGTAAATTT
    GTGGGATATC CTGGAAACTA CGCCTACATG TTTGGGCCTT GGAGTGGACT GAGAAATGAA
    GAGTGTGAGC CTGGCGGCTG TCTGATCGAG CTGACCACTC AGTTAGTGAT CGTGATGGTG
    GGCAAGCAGG TGTGGGGGAA CATTCAAGAA GCTCTCGTCC CCTGGCTGCG TAACTGGTGG
    GTGAGCAGAA GTGCTCGTAA TCATCCGGAG AGTCTGTACA GCCGCTGGGA GCAGGATCAC
    GATCTGCAGA ACTTGGGGCA GCTCGGCCTC TTCTACGAGT ACCTGGAGAT GGTGATTCAG
    TTTGGCTTCA TCACTCTGTT CGTGGCCTCT TTTCCTCTGG CTCCGCTCCT GGCTCTGATG
    AATAATATCC TGGAGGTGCG CGTGGACGCC TGGAAGTTCA CCACTCAGTT CCGCAGACCC
    GTCGCTGCTA AAGCGCACAG CATCGGCGCC TGGGAGGAAA TCCTCAACAT GATCGCAGTC
    TTCTCCGTCG TCACCAACGC TTTCATCGTG TCCTTCACGT CTGACATGAT CCCGCGGCTG
    GTGTACCAGT ACGTGCATAA TACGGGCAAT GCGGCCTCTA TGTCGGGATA CATCACCCAC
    AGCCTCTCCA TCTACAACAT CTCAGAGTTT CCTCCCGAAA ACCGTCCGGA TGATGACGAA
    AACCCAGACT GGTTCAACGC CGGCAACTAC ACCACCTGCA GGTATCGAGA TTATCGATAT
    CCTCCAGGAC ACGAGAAGCA GTACACCCAC AACATGGACT TCTGGCACAT CCTCGCCGCC
    AAACTGGCAT TCATCATCAT CATGGAGCAT GTGGTGTTTG TGGTGAAGTT TTTCGTGGCG
    TGGTTGATCC CCGACGTGCC TTCAGACGTA AAAGCGCGTG TAAAGCGCGA GCGTTACCTC
    ATCCAGGAGT ATCTGCACGA CTATGAGGTG GAAAAACTGA AGAACCAGCT CAGCCTGAAC
    ACTGACGTCC ACAATGACAT TACGGCATGC ACCTGTCCCA CCACAGACGC CATCCCCAAA
    CTGGAGGTAT TATCAGACTG TCTGACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326433.1
    CDS169..2958
    Translation

    Target ORF information:

    RefSeq Version XM_021470758.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 5a (ano5a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATCGCG GATCGCAAAA GTCGAGTGAT GAGACGCTTA TAGAAATGAG CCGAACTGCT 
    TGGTCCAGCG GCGAAGACAA CAATGGCTTC CATTCCAATT CGGATAAGGA GACGTTTCAA
    CTTGAGAATG CTGAGATCTG GAGAAAGAGA GCTTTATTTC TGAAGTATCG CTCCAGGGTG
    GATAAACAGC AGCAGAGTAA GGACTCTGTG TACTTCAGGG ATGGTGTACG GAGGATAGAT
    TTCGTCCTGT CTTACATCGA CGACAAGGAT GGAGAAAAGA AACAGGACCG AAGGAAGGTG
    TTTGAGGCTA ACTTACAGAA AGCCGGACTG GAGGTGGAGA CCGAGGACAA ATCTGAGTCT
    GAGGATGGAA AGACGTACTT CTTGAAGATC CACGTTCCAT GGGAGGTGCT GGCGACATAT
    GCCGACGTCC TGAAAATCAA AGTGCCTTTC AAAGCCAACG ATATTCCTGA AAATAAAGAG
    ATACCGATGG GCTGGCTCTT CACGCCCTTC CGCCTGCCCG ACCATGTGAT GAATCCAGAG
    CCCGACTTCT TCACCTCCAC GTTCGACAAG AGCAAGATCG ACTTCTTCCT TATTGAAGAT
    AAAGAAACCC TCTTTCCTGC GTCCACGCGC AACAGGATTG TTTATTACCT CCTTTCCCGA
    ATCCCGTACA GCACAGAAGA CAAAGATAAG AAGGGTATTA AGAGGTTGCT GAACAACGGG
    ACCTACACGT CCGCCTTCCC TCTGCATGAC TGTCGATATT GGACCAGGTC TCGTGATGAT
    AACTGTGAGA GCGAACGCTA TTCTCTGTAT AAAAACTGGG CTCGCTTCAG CAGCTTCTAC
    AAGGAGCAGC CGCTCAACCT CATTCGGAAG TACTATGGAG AGAAGATCGG CATCTACTTT
    GCTTGGCTGG GTTTTTACAC GGAGATGCTG TCCTATGCTG CGGTTGTGGG TTTACTCTGC
    TTCATCTATG GAGTGGCCAC CTTTGACGAA AACATCTGGA GTCGAGAGAT CTGCAACGAG
    ACCATCGGTG GGCAGATTGT CATGTGTCCG CTCTGTGATA AGAAATGCGG CTACTGGAAG
    CTCAGCACTA CCTGCAGCTC TTCATGGCAA TCTTATTTAT TTGACAATGC AGCGACGGTG
    TTCTTTGCTA TATTCATGGG AATATGGGTC ACTTTATTCC TGGAGTTCTG GAAGCGGCGT
    CAGGCCCGGC TGGAGTACGA GTGGGATCTG GTGGACTTTG AGGAAGAGCA ACAGCAGCTC
    CAGCTCAGAC CCGAATACGA GTCCAAGTGC ACCAATCGGC GACTCAACCG CGTCACACAG
    GAGATGGAGC CTTATTTACC TCTAACAAGC AAGTGTGCTC GTTCAATACT GTCTGGGGCC
    ACCGTCCTCT TCTGGATCTC TCTGATTATC GCCAGTATTA TCGGTGTGAT TGCGTATCGT
    CTGGCGGTGT TCGCTGCATT CGCGAGCATC ATGAAGGACA GTCCCACTAA TAAGCTGGAG
    ATAGTGGGAA ACCTGATCAC GCCTCAGTTC GCTACGTCTG TAACGGCGTC CTGCATCAAC
    TTTGTCATCA TCATGGTGCT GAACTTCCTG TATGAGCGCG TCGCCATCAA GATCACTGAT
    ATGGAGGTCC CTAAAACACA CGTCGAGTAC GAGAACAAGC TGACGATAAA GATGTTCCTC
    TTCCAGTTCG TCAACTATTA TTCCTCCTGC TTCTACGTTG CTTTCTTCAA GGGTAAATTT
    GTGGGATATC CTGGAAACTA CGCCTACATG TTTGGGCCTT GGAGTGGACT GAGAAATGAA
    GAGTGTGAGC CTGGCGGCTG TCTGATCGAG CTGACCACTC AGTTAGTGAT CGTGATGGTG
    GGCAAGCAGG TGTGGGGGAA CATTCAAGAA GCTCTCGTCC CCTGGCTGCG TAACTGGTGG
    GTGAGCAGAA GTGCTCGTAA TCATCCGGAG AGTCTGTACA GCCGCTGGGA GCAGGATCAC
    GATCTGCAGA ACTTGGGGCA GCTCGGCCTC TTCTACGAGT ACCTGGAGAT GGTGATTCAG
    TTTGGCTTCA TCACTCTGTT CGTGGCCTCT TTTCCTCTGG CTCCGCTCCT GGCTCTGATG
    AATAATATCC TGGAGGTGCG CGTGGACGCC TGGAAGTTCA CCACTCAGTT CCGCAGACCC
    GTCGCTGCTA AAGCGCACAG CATCGGCGCC TGGGAGGAAA TCCTCAACAT GATCGCAGTC
    TTCTCCGTCG TCACCAACGC TTTCATCGTG TCCTTCACGT CTGACATGAT CCCGCGGCTG
    GTGTACCAGT ACGTGCATAA TACGGGCAAT GCGGCCTCTA TGTCGGGATA CATCACCCAC
    AGCCTCTCCA TCTACAACAT CTCAGAGTTT CCTCCCGAAA ACCGTCCGGA TGATGACGAA
    AACCCAGACT GGTTCAACGC CGGCAACTAC ACCACCTGCA GGTATCGAGA TTATCGATAT
    CCTCCAGGAC ACGAGAAGCA GTACACCCAC AACATGGACT TCTGGCACAT CCTCGCCGCC
    AAACTGGCAT TCATCATCAT CATGGAGCAT GTGGTGTTTG TGGTGAAGTT TTTCGTGGCG
    TGGTTGATCC CCGACGTGCC TTCAGACGTA AAAGCGCGTG TAAAGCGCGA GCGTTACCTC
    ATCCAGGAGT ATCTGCACGA CTATGAGGTG GAAAAACTGA AGAACCAGCT CAGCCTGAAC
    ACTGACGTCC ACAATGACAT TACGGCATGC ACCTGTCCCA CCACAGACGC CATCCCCAAA
    CTGGAGGTAT TATCAGACTG TCTGACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa50065
    Clone ID Related Accession (Same CDS sequence) XM_021470760.1
    Accession Version XM_021470760.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product anoctamin-5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGCAGC ACTTGATAGA CGGTTTGGAG GTGGATAAAC AGCAGCAGAG TAAGGACTCT 
    GTGTACTTCA GGGATGGTGT ACGGAGGATA GATTTCGTCC TGTCTTACAT CGACGACAAG
    GATGGAGAAA AGAAACAGGA CCGAAGGAAG GTGTTTGAGG CTAACTTACA GAAAGCCGGA
    CTGGAGGTGG AGACCGAGGA CAAATCTGAG TCTGAGGATG GAAAGACGTA CTTCTTGAAG
    ATCCACGTTC CATGGGAGGT GCTGGCGACA TATGCCGACG TCCTGAAAAT CAAAGTGCCT
    TTCAAAGCCA ACGATATTCC TGAAAATAAA GAGATACCGA TGGGCTGGCT CTTCACGCCC
    TTCCGCCTGC CCGACCATGT GATGAATCCA GAGCCCGACT TCTTCACCTC CACGTTCGAC
    AAGAGCAAGA TCGACTTCTT CCTTATTGAA GATAAAGAAA CCCTCTTTCC TGCGTCCACG
    CGCAACAGGA TTGTTTATTA CCTCCTTTCC CGAATCCCGT ACAGCACAGA AGACAAAGAT
    AAGAAGGGTA TTAAGAGGTT GCTGAACAAC GGGACCTACA CGTCCGCCTT CCCTCTGCAT
    GACTGTCGAT ATTGGACCAG GTCTCGTGAT GATAACTGTG AGAGCGAACG CTATTCTCTG
    TATAAAAACT GGGCTCGCTT CAGCAGCTTC TACAAGGAGC AGCCGCTCAA CCTCATTCGG
    AAGTACTATG GAGAGAAGAT CGGCATCTAC TTTGCTTGGC TGGGTTTTTA CACGGAGATG
    CTGTCCTATG CTGCGGTTGT GGGTTTACTC TGCTTCATCT ATGGAGTGGC CACCTTTGAC
    GAAAACATCT GGAGTCGAGA GATCTGCAAC GAGACCATCG GTGGGCAGAT TGTCATGTGT
    CCGCTCTGTG ATAAGAAATG CGGCTACTGG AAGCTCAGCA CTACCTGCAG CTCTTCATGG
    CAATCTTATT TATTTGACAA TGCAGCGACG GTGTTCTTTG CTATATTCAT GGGAATATGG
    GTCACTTTAT TCCTGGAGTT CTGGAAGCGG CGTCAGGCCC GGCTGGAGTA CGAGTGGGAT
    CTGGTGGACT TTGAGGAAGA GCAACAGCAG CTCCAGCTCA GACCCGAATA CGAGTCCAAG
    TGCACCAATC GGCGACTCAA CCGCGTCACA CAGGAACAGG AGTGGGTTCT AGACCAGACT
    CCAGCCGATA AAGCAGGCAG GTTTGTCCTG TGCTGGACCA CTGTTCTCTT ATGGATCTCT
    CTGATTATCG CCAGTATTAT CGGTGTGATT GCGTATCGTC TGGCGGTGTT CGCTGCATTC
    GCGAGCATCA TGAAGGACAG TCCCACTAAT AAGCTGGAGA TAGTGGGAAA CCTGATCACG
    CCTCAGTTCG CTACGTCTGT AACGGCGTCC TGCATCAACT TTGTCATCAT CATGGTGCTG
    AACTTCCTGT ATGAGCGCGT CGCCATCAAG ATCACTGATA TGGAGGTCCC TAAAACACAC
    GTCGAGTACG AGAACAAGCT GACGATAAAG ATGTTCCTCT TCCAGTTCGT CAACTATTAT
    TCCTCCTGCT TCTACGTTGC TTTCTTCAAG GGTAAATTTG TGGGATATCC TGGAAACTAC
    GCCTACATGT TTGGGCCTTG GAGTGGACTG AGAAATGAAG AGTGTGAGCC TGGCGGCTGT
    CTGATCGAGC TGACCACTCA GTTAGTGATC GTGATGGTGG GCAAGCAGGT GTGGGGGAAC
    ATTCAAGAAG CTCTCGTCCC CTGGCTGCGT AACTGGTGGG TGAGCAGAAG TGCTCGTAAT
    CATCCGGAGA GTCTGTACAG CCGCTGGGAG CAGGATCACG ATCTGCAGAA CTTGGGGCAG
    CTCGGCCTCT TCTACGAGTA CCTGGAGATG GTGATTCAGT TTGGCTTCAT CACTCTGTTC
    GTGGCCTCTT TTCCTCTGGC TCCGCTCCTG GCTCTGATGA ATAATATCCT GGAGGTGCGC
    GTGGACGCCT GGAAGTTCAC CACTCAGTTC CGCAGACCCG TCGCTGCTAA AGCGCACAGC
    ATCGGCGCCT GGGAGGAAAT CCTCAACATG ATCGCAGTCT TCTCCGTCGT CACCAACGCT
    TTCATCGTGT CCTTCACGTC TGACATGATC CCGCGGCTGG TGTACCAGTA CGTGCATAAT
    ACGGGCAATG CGGCCTCTAT GTCGGGATAC ATCACCCACA GCCTCTCCAT CTACAACATC
    TCAGAGTTTC CTCCCGAAAA CCGTCCGGAT GATGACGAAA ACCCAGACTG GTTCAACGCC
    GGCAACTACA CCACCTGCAG GTATCGAGAT TATCGATATC CTCCAGGACA CGAGAAGCAG
    TACACCCACA ACATGGACTT CTGGCACATC CTCGCCGCCA AACTGGCATT CATCATCATC
    ATGGAGCATG TGGTGTTTGT GGTGAAGTTT TTCGTGGCGT GGTTGATCCC CGACGTGCCT
    TCAGACGTAA AAGCGCGTGT AAAGCGCGAG CGTTACCTCA TCCAGGAGTA TCTGCACGAC
    TATGAGGTGG AAAAACTGAA GAACCAGCTC AGCCTGAACA CTGACGTCCA CAATGACATT
    ACGGCATGCA CCTGTCCCAC CACAGACGCC ATCCCCAAAC TGGAGGTATT ATCAGACTGT
    CTGACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326435.1
    CDS109..2757
    Translation

    Target ORF information:

    RefSeq Version XM_021470760.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio anoctamin 5a (ano5a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470760.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGCAGC ACTTGATAGA CGGTTTGGAG GTGGATAAAC AGCAGCAGAG TAAGGACTCT 
    GTGTACTTCA GGGATGGTGT ACGGAGGATA GATTTCGTCC TGTCTTACAT CGACGACAAG
    GATGGAGAAA AGAAACAGGA CCGAAGGAAG GTGTTTGAGG CTAACTTACA GAAAGCCGGA
    CTGGAGGTGG AGACCGAGGA CAAATCTGAG TCTGAGGATG GAAAGACGTA CTTCTTGAAG
    ATCCACGTTC CATGGGAGGT GCTGGCGACA TATGCCGACG TCCTGAAAAT CAAAGTGCCT
    TTCAAAGCCA ACGATATTCC TGAAAATAAA GAGATACCGA TGGGCTGGCT CTTCACGCCC
    TTCCGCCTGC CCGACCATGT GATGAATCCA GAGCCCGACT TCTTCACCTC CACGTTCGAC
    AAGAGCAAGA TCGACTTCTT CCTTATTGAA GATAAAGAAA CCCTCTTTCC TGCGTCCACG
    CGCAACAGGA TTGTTTATTA CCTCCTTTCC CGAATCCCGT ACAGCACAGA AGACAAAGAT
    AAGAAGGGTA TTAAGAGGTT GCTGAACAAC GGGACCTACA CGTCCGCCTT CCCTCTGCAT
    GACTGTCGAT ATTGGACCAG GTCTCGTGAT GATAACTGTG AGAGCGAACG CTATTCTCTG
    TATAAAAACT GGGCTCGCTT CAGCAGCTTC TACAAGGAGC AGCCGCTCAA CCTCATTCGG
    AAGTACTATG GAGAGAAGAT CGGCATCTAC TTTGCTTGGC TGGGTTTTTA CACGGAGATG
    CTGTCCTATG CTGCGGTTGT GGGTTTACTC TGCTTCATCT ATGGAGTGGC CACCTTTGAC
    GAAAACATCT GGAGTCGAGA GATCTGCAAC GAGACCATCG GTGGGCAGAT TGTCATGTGT
    CCGCTCTGTG ATAAGAAATG CGGCTACTGG AAGCTCAGCA CTACCTGCAG CTCTTCATGG
    CAATCTTATT TATTTGACAA TGCAGCGACG GTGTTCTTTG CTATATTCAT GGGAATATGG
    GTCACTTTAT TCCTGGAGTT CTGGAAGCGG CGTCAGGCCC GGCTGGAGTA CGAGTGGGAT
    CTGGTGGACT TTGAGGAAGA GCAACAGCAG CTCCAGCTCA GACCCGAATA CGAGTCCAAG
    TGCACCAATC GGCGACTCAA CCGCGTCACA CAGGAACAGG AGTGGGTTCT AGACCAGACT
    CCAGCCGATA AAGCAGGCAG GTTTGTCCTG TGCTGGACCA CTGTTCTCTT ATGGATCTCT
    CTGATTATCG CCAGTATTAT CGGTGTGATT GCGTATCGTC TGGCGGTGTT CGCTGCATTC
    GCGAGCATCA TGAAGGACAG TCCCACTAAT AAGCTGGAGA TAGTGGGAAA CCTGATCACG
    CCTCAGTTCG CTACGTCTGT AACGGCGTCC TGCATCAACT TTGTCATCAT CATGGTGCTG
    AACTTCCTGT ATGAGCGCGT CGCCATCAAG ATCACTGATA TGGAGGTCCC TAAAACACAC
    GTCGAGTACG AGAACAAGCT GACGATAAAG ATGTTCCTCT TCCAGTTCGT CAACTATTAT
    TCCTCCTGCT TCTACGTTGC TTTCTTCAAG GGTAAATTTG TGGGATATCC TGGAAACTAC
    GCCTACATGT TTGGGCCTTG GAGTGGACTG AGAAATGAAG AGTGTGAGCC TGGCGGCTGT
    CTGATCGAGC TGACCACTCA GTTAGTGATC GTGATGGTGG GCAAGCAGGT GTGGGGGAAC
    ATTCAAGAAG CTCTCGTCCC CTGGCTGCGT AACTGGTGGG TGAGCAGAAG TGCTCGTAAT
    CATCCGGAGA GTCTGTACAG CCGCTGGGAG CAGGATCACG ATCTGCAGAA CTTGGGGCAG
    CTCGGCCTCT TCTACGAGTA CCTGGAGATG GTGATTCAGT TTGGCTTCAT CACTCTGTTC
    GTGGCCTCTT TTCCTCTGGC TCCGCTCCTG GCTCTGATGA ATAATATCCT GGAGGTGCGC
    GTGGACGCCT GGAAGTTCAC CACTCAGTTC CGCAGACCCG TCGCTGCTAA AGCGCACAGC
    ATCGGCGCCT GGGAGGAAAT CCTCAACATG ATCGCAGTCT TCTCCGTCGT CACCAACGCT
    TTCATCGTGT CCTTCACGTC TGACATGATC CCGCGGCTGG TGTACCAGTA CGTGCATAAT
    ACGGGCAATG CGGCCTCTAT GTCGGGATAC ATCACCCACA GCCTCTCCAT CTACAACATC
    TCAGAGTTTC CTCCCGAAAA CCGTCCGGAT GATGACGAAA ACCCAGACTG GTTCAACGCC
    GGCAACTACA CCACCTGCAG GTATCGAGAT TATCGATATC CTCCAGGACA CGAGAAGCAG
    TACACCCACA ACATGGACTT CTGGCACATC CTCGCCGCCA AACTGGCATT CATCATCATC
    ATGGAGCATG TGGTGTTTGT GGTGAAGTTT TTCGTGGCGT GGTTGATCCC CGACGTGCCT
    TCAGACGTAA AAGCGCGTGT AAAGCGCGAG CGTTACCTCA TCCAGGAGTA TCTGCACGAC
    TATGAGGTGG AAAAACTGAA GAACCAGCTC AGCCTGAACA CTGACGTCCA CAATGACATT
    ACGGCATGCA CCTGTCCCAC CACAGACGCC ATCCCCAAAC TGGAGGTATT ATCAGACTGT
    CTGACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.