ano5b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ano5b
Entrez Gene ID	560102
Full Name	anoctamin 5b
Synonyms	Gdd1,si:packt73.3,si:packt90.1,si:xx-packt90.1,wu:fb74d12,zgc:158430
General protein information	Preferred Names anoctamin 5b Names anoctamin 5b integral membrane protein GDD1 tmem16eb
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005168891.4	XP_005168948.1	anoctamin 5b isoform X1
NM_001079983.1	NP_001073452.1	anoctamin 5b
XM_005168891.3	XP_005168948.1	anoctamin 5b isoform X1
XM_009303225.2	XP_009301500.1	anoctamin 5b isoform X2
XM_009303225.3	XP_009301500.1	anoctamin 5b isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ano5	XP_006229328.1
Danio rerio (zebrafish)	ano5b	NP_001073452.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010474	XP_311470.4
	LOC704166	XP_002799660.1
Canis lupus familiaris (dog)	ANO5	NP_001161885.1
Homo sapiens (human)	ANO5	NP_001136121.1
Mus musculus (house mouse)	Ano5	NP_808362.2
Danio rerio (zebrafish)	ano5a	XP_005163279.1
Drosophila melanogaster (fruit fly)	CG6938	NP_648535.2
Bos taurus (cattle)	ANO5	NP_001161878.1
Xenopus tropicalis (tropical clawed frog)	ano5	XP_002933920.2
Pan troglodytes (chimpanzee)	ANO5	XP_003313017.1
Gallus gallus (chicken)	ANO5	NP_001161856.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Comparative genomic analysis of teleost fish bmal genes.
Wang H
Genetica136(1)149-61(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ano5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ano5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36520	NM_001079983.1 Latest version!	Danio rerio anoctamin 5b (ano5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa13081	XM_005168891.4 Latest version!	Danio rerio anoctamin 5b (ano5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa43238	XM_009303225.3 Latest version!	Danio rerio anoctamin 5b (ano5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa13081	XM_005168891.3	Danio rerio anoctamin 5b (ano5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa43238	XM_009303225.2	Danio rerio anoctamin 5b (ano5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ano5b ( NM_001079983.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073452.1 Danio rerio anoctamin 5b (ano5b), mRNA.

Price & Availability↑

CloneID	ODa36520
Clone ID Related Accession (Same CDS sequence)	NM_001079983.1
Accession Version	NM_001079983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Danio rerio(zebrafish)
Product	anoctamin 5b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC128870.1. On Mar 11, 2007 this sequence version replaced XM_683264.2. ##Evidence-Data-START## Transcript exon combination :: BC128870.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACCAGCG CGCTGGATTC TCAGATTGAA GGCAATAATG GCAGCATTAA CTCAATCTCT 
GACCGAGGGA TTCAGCTGCC TGGTCATCGG GAGATAGACA AGTTTGATCA GAACAGAGAC 
TCTGTGTTCT TCAAGGATGG CGTACGGCGC ATTGACTTCG TCCTGTCCTA CGTCGATGAC 
AAAGAGGGAG ACAAAAAAGC GGAGAGGAGA AGAGAGTTTG AAGCCAACCT AGAGAAAGCT 
GGTCTCGAGC TGGAGACTGA AGATAAATCG GAGTCAGATG ATCGTAAGAC ACATTATCTG 
AAGATTCACG CTCCATGGGA GGTGTTGGCC ACTTACGCTG ATGTACTAAA GATTAAAGTG 
CCCTTTAAAG TGAGCGACAT CCCCAAAGCT CGAGAGGTTC CTCTGGAGTG GCTCAGTCAC 
CCTTTCCGGC TGCCAGAAAA CATCATGAGG CCCGAGCCGG ACTACTTCAC TGCACCCTTT 
GACAAGAGCA AGGTGGACTT CTTCCTCATT GATGATAAAG ACACCTTTTT CCCACCTTCC 
ACACGCAACA GGATTGTGTA CTACATCCTG ACTCGCTGCC CATACTACAA AGAAGACCGA 
AAAGAGAAGG ATAAGACTGG GATTAATCGG CTGTTGAATA ACGGCACATA CACCTCTGCT 
TATCCGCTTC ATGATTGTCG TTATTGGAAG AAGGCTCAGG ACATGCAGTG TGAGAGTGAA 
AGGTATCATC TGTATCGCTA TTGGGCTCGA TTCCTCTGCT TCTACAAAGA GCAGCCGCTC 
AATCTGATCA AGAAGTACTA CGGTGAGAAG ATCGGTATCT ACTTCGCCTG GCTGGGCTTT 
TACACAGAGA TGCTGTTTTA TGCTGCAGTC ATGGGTGTCA TCTGTTTTGT TTATGGTGTC 
CTCAGTTACG AAGACAACAT CACAAGTAAG GAGATCTGTG ACCCAAAAAT AGGAGGCATG 
ATTGTCATGT GTCCGCTCTG TGACAAAAAG TGCAGCTACT GGAAACTCAA CTCAACATGT 
CTGTCCTCCT GGCAATCCCA TCTCTTTGAT AATGAAGGGA CTGTGTTTTT TGCCATGTTC 
ATGGGCATTT GGGTGACCCT CTTCCTGGAG TTCTGGAAGC GACGGCAGGC CCGTCTGGAG 
TATGAGTGGG ACCTTGTGGA TTTTGAGGAG GAGCAACAGC AGCTCCAGAT CAGGCCAGAG 
TATGAGCAGA AGTGCACAGG CCGCAGACTC AACCGCATCA CACAGGAAAT GGAACCTTAT 
CTTCCTTTCC CCAGCAAATG TGCTCGATTC TGCCTATCTG GAGCAACTGT ACTCTTCTGG 
ACGTGTCTCA TAGTGGCCTG CATAATGGGA GTGATCGCCT ACAGATTAGC GGTGTATGCA 
GCGTTCGCCA GCGTCATGAA AGACAGTTCC ACCAGTAAGA TCCAGCTGGT TGGGTCACTC 
ATCACCCCTC AGCTGGCCAC TTCCGTTACT GCTTCCTGCA TCAACTTCGT CATTATCCTC 
ATCCTCAATT TCCTCTATGA GCATGTGGCT ATCTGGATCA CTGACATGGA AATCCCCAAG 
ACCCATCTGG AGTATGAGAA CAAGCTGACC ATGAAGATGT TCATGTTCCA GTTTGTGAAC 
TACTACTCTT CCTGTTTCTA TGTGGCCTTC TTTAAGGGAA AGTTTGTGGG TTATCCGGGA 
AACTACAGTT ATATGTTTGG AAAGTGGAGC ACTTTGAGAA ATGAGGAGTG TGCTCCTGGA 
GGTTGTCTGA TTGAGCTGAC CACTCAGCTG CTTATTGTCA TGGCTGGGAA ACAAATGGTC 
GGGAACGTCC AAGAGGCTCT TTTGCCACTT GTCCGAAACT GGTGGAGCAG CAGGAAAGGC 
CGCAGTCATC CAGAAAGCAC GTATAGTAGA TGGGAGCAGG ACCATGACCT CCAGAACTTC 
AGCCAGTTTG GACTCTTCTA TGAGTATCTG GAGATGGTGA TCCAGTTTGG CTTCATAACA 
CTTTTCGTGG CCTCCTTTCC ACTGGCTCCT CTTCTTGCCC TCTTCAACAA CATCCTGGAG 
GTGCGAGTGG ATGCTTGGAA ATTCACCACA CAGTTCAGAC GACCGATGGC AGCCAAAGCA 
CGCAACATCG GAGCTTGGGA AGAGATCCTT AATGTGGTGG CCATCATGTC CGTGGTCACA 
AATGCCTTCA TCATGGCGTT CACCTCAGAT ATGATCCCTC GTCTGGTCTA TCTGTACGCT 
TATCACCCAG GCATTGAGGC CAATATGACC GGTTATATCA CCAACAGCCT CTCGATTTAC 
AACATCTCTC AGATCCCAGA AGATAACCTG CCTGAAGCTG GAGAAAACCC ATCCTGGTTT 
AACAGCTCCA CCATCACCAC ATGCAGGTAT CGGGACTACA GATACCCACC TGGCCATCTG 
AGGCAGTACA CGCACACCAT GCAGTTCTGG CACATCCTGG CTGCTAAGCT CGCCTTCATC 
ATTATTATGG AACATGTTGT GTTTGTGGTA AAGTTCTTCG TGGCGTGGCT CATCCCAGAT 
GTGCCTTCAG AAGTGAAAGC CCGTATAAAG CGTGAGCGCT TTCTGGTCCA GGAATACCTG 
CACAACTATG AAGTGGAGAA ACTGAAGATG CAGCTAAGCC AGAGTTTCTG CCTATCCACA 
GAAACGGCCT CGCTGCTGCC CTCTAGTCCA AACAAACACC AGGTGATTTC TGAATGTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073452.1
CDS	223..2925
Translation	MTSALDSQIEGNNGSINSISDRGIQLPGHREIDKFDQNRDSVFFKDGVRRIDFVLSYVDDKEGDKKAERRREFEANLEKAGLELETEDKSESDDRKTHYLKIHAPWEVLATYADVLKIKVPFKVSDIPKAREVPLEWLSHPFRLPENIMRPEPDYFTAPFDKSKVDFFLIDDKDTFFPPSTRNRIVYYILTRCPYYKEDRKEKDKTGINRLLNNGTYTSAYPLHDCRYWKKAQDMQCESERYHLYRYWARFLCFYKEQPLNLIKKYYGEKIGIYFAWLGFYTEMLFYAAVMGVICFVYGVLSYEDNITSKEICDPKIGGMIVMCPLCDKKCSYWKLNSTCLSSWQSHLFDNEGTVFFAMFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQIRPEYEQKCTGRRLNRITQEMEPYLPFPSKCARFCLSGATVLFWTCLIVACIMGVIAYRLAVYAAFASVMKDSSTSKIQLVGSLITPQLATSVTASCINFVIILILNFLYEHVAIWITDMEIPKTHLEYENKLTMKMFMFQFVNYYSSCFYVAFFKGKFVGYPGNYSYMFGKWSTLRNEECAPGGCLIELTTQLLIVMAGKQMVGNVQEALLPLVRNWWSSRKGRSHPESTYSRWEQDHDLQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNILEVRVDAWKFTTQFRRPMAAKARNIGAWEEILNVVAIMSVVTNAFIMAFTSDMIPRLVYLYAYHPGIEANMTGYITNSLSIYNISQIPEDNLPEAGENPSWFNSSTITTCRYRDYRYPPGHLRQYTHTMQFWHILAAKLAFIIIMEHVVFVVKFFVAWLIPDVPSEVKARIKRERFLVQEYLHNYEVEKLKMQLSQSFCLSTETASLLPSSPNKHQVISECV

Target ORF information:

RefSeq Version	NM_001079983.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anoctamin 5b (ano5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079983.1

ORF Insert Sequence:

ATGACCAGCG CGCTGGATTC TCAGATTGAA GGCAATAATG GCAGCATTAA CTCAATCTCT 
GACCGAGGGA TTCAGCTGCC TGGTCATCGG GAGATAGACA AGTTTGATCA GAACAGAGAC 
TCTGTGTTCT TCAAGGATGG CGTACGGCGC ATTGACTTCG TCCTGTCCTA CGTCGATGAC 
AAAGAGGGAG ACAAAAAAGC GGAGAGGAGA AGAGAGTTTG AAGCCAACCT AGAGAAAGCT 
GGTCTCGAGC TGGAGACTGA AGATAAATCG GAGTCAGATG ATCGTAAGAC ACATTATCTG 
AAGATTCACG CTCCATGGGA GGTGTTGGCC ACTTACGCTG ATGTACTAAA GATTAAAGTG 
CCCTTTAAAG TGAGCGACAT CCCCAAAGCT CGAGAGGTTC CTCTGGAGTG GCTCAGTCAC 
CCTTTCCGGC TGCCAGAAAA CATCATGAGG CCCGAGCCGG ACTACTTCAC TGCACCCTTT 
GACAAGAGCA AGGTGGACTT CTTCCTCATT GATGATAAAG ACACCTTTTT CCCACCTTCC 
ACACGCAACA GGATTGTGTA CTACATCCTG ACTCGCTGCC CATACTACAA AGAAGACCGA 
AAAGAGAAGG ATAAGACTGG GATTAATCGG CTGTTGAATA ACGGCACATA CACCTCTGCT 
TATCCGCTTC ATGATTGTCG TTATTGGAAG AAGGCTCAGG ACATGCAGTG TGAGAGTGAA 
AGGTATCATC TGTATCGCTA TTGGGCTCGA TTCCTCTGCT TCTACAAAGA GCAGCCGCTC 
AATCTGATCA AGAAGTACTA CGGTGAGAAG ATCGGTATCT ACTTCGCCTG GCTGGGCTTT 
TACACAGAGA TGCTGTTTTA TGCTGCAGTC ATGGGTGTCA TCTGTTTTGT TTATGGTGTC 
CTCAGTTACG AAGACAACAT CACAAGTAAG GAGATCTGTG ACCCAAAAAT AGGAGGCATG 
ATTGTCATGT GTCCGCTCTG TGACAAAAAG TGCAGCTACT GGAAACTCAA CTCAACATGT 
CTGTCCTCCT GGCAATCCCA TCTCTTTGAT AATGAAGGGA CTGTGTTTTT TGCCATGTTC 
ATGGGCATTT GGGTGACCCT CTTCCTGGAG TTCTGGAAGC GACGGCAGGC CCGTCTGGAG 
TATGAGTGGG ACCTTGTGGA TTTTGAGGAG GAGCAACAGC AGCTCCAGAT CAGGCCAGAG 
TATGAGCAGA AGTGCACAGG CCGCAGACTC AACCGCATCA CACAGGAAAT GGAACCTTAT 
CTTCCTTTCC CCAGCAAATG TGCTCGATTC TGCCTATCTG GAGCAACTGT ACTCTTCTGG 
ACGTGTCTCA TAGTGGCCTG CATAATGGGA GTGATCGCCT ACAGATTAGC GGTGTATGCA 
GCGTTCGCCA GCGTCATGAA AGACAGTTCC ACCAGTAAGA TCCAGCTGGT TGGGTCACTC 
ATCACCCCTC AGCTGGCCAC TTCCGTTACT GCTTCCTGCA TCAACTTCGT CATTATCCTC 
ATCCTCAATT TCCTCTATGA GCATGTGGCT ATCTGGATCA CTGACATGGA AATCCCCAAG 
ACCCATCTGG AGTATGAGAA CAAGCTGACC ATGAAGATGT TCATGTTCCA GTTTGTGAAC 
TACTACTCTT CCTGTTTCTA TGTGGCCTTC TTTAAGGGAA AGTTTGTGGG TTATCCGGGA 
AACTACAGTT ATATGTTTGG AAAGTGGAGC ACTTTGAGAA ATGAGGAGTG TGCTCCTGGA 
GGTTGTCTGA TTGAGCTGAC CACTCAGCTG CTTATTGTCA TGGCTGGGAA ACAAATGGTC 
GGGAACGTCC AAGAGGCTCT TTTGCCACTT GTCCGAAACT GGTGGAGCAG CAGGAAAGGC 
CGCAGTCATC CAGAAAGCAC GTATAGTAGA TGGGAGCAGG ACCATGACCT CCAGAACTTC 
AGCCAGTTTG GACTCTTCTA TGAGTATCTG GAGATGGTGA TCCAGTTTGG CTTCATAACA 
CTTTTCGTGG CCTCCTTTCC ACTGGCTCCT CTTCTTGCCC TCTTCAACAA CATCCTGGAG 
GTGCGAGTGG ATGCTTGGAA ATTCACCACA CAGTTCAGAC GACCGATGGC AGCCAAAGCA 
CGCAACATCG GAGCTTGGGA AGAGATCCTT AATGTGGTGG CCATCATGTC CGTGGTCACA 
AATGCCTTCA TCATGGCGTT CACCTCAGAT ATGATCCCTC GTCTGGTCTA TCTGTACGCT 
TATCACCCAG GCATTGAGGC CAATATGACC GGTTATATCA CCAACAGCCT CTCGATTTAC 
AACATCTCTC AGATCCCAGA AGATAACCTG CCTGAAGCTG GAGAAAACCC ATCCTGGTTT 
AACAGCTCCA CCATCACCAC ATGCAGGTAT CGGGACTACA GATACCCACC TGGCCATCTG 
AGGCAGTACA CGCACACCAT GCAGTTCTGG CACATCCTGG CTGCTAAGCT CGCCTTCATC 
ATTATTATGG AACATGTTGT GTTTGTGGTA AAGTTCTTCG TGGCGTGGCT CATCCCAGAT 
GTGCCTTCAG AAGTGAAAGC CCGTATAAAG CGTGAGCGCT TTCTGGTCCA GGAATACCTG 
CACAACTATG AAGTGGAGAA ACTGAAGATG CAGCTAAGCC AGAGTTTCTG CCTATCCACA 
GAAACGGCCT CGCTGCTGCC CTCTAGTCCA AACAAACACC AGGTGATTTC TGAATGTGTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano5b ( XM_005168891.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168948.1 Danio rerio anoctamin 5b (ano5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13081
Clone ID Related Accession (Same CDS sequence)	XM_005168891.4 , XM_005168891.3
Accession Version	XM_005168891.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	anoctamin 5b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168891.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TAACGGGCAA GACGAAAGAA GCCACTCTTA TAGAAATGAC CAGCGCGCTG 
GATTCTCAGA TTGAAGATGG CTGTGCCGTT CTGACGGGCT ACAGGAGGAC CTTACCAGCA 
GGCAATAATG GCAGCATTAA CTCAATCTCT GACCGAGGGA TTCAGCTGCC TGGTCATCGG 
GAGATAGACA AGTTTGATCA GAACAGAGAC TCTGTGTTCT TCAAGGATGG CGTACGGCGC 
ATTGACTTCG TCCTGTCCTA CGTCGATGAC AAAGAGGGAG ACAAAAAAGC GGAGAGGAGA 
AGAGAGTTTG AAGCCAACCT AGAGAAAGCT GGTCTCGAGC TGGAGACTGA AGATAAATCG 
GAGTCAGATG ATCGTAAGAC ACATTATCTG AAGATTCACG CTCCATGGGA GGTGTTGGCC 
ACTTACGCTG ATGTACTAAA GATTAAAGTG CCCTTTAAAG TGAGCGACAT CCCCAAAGCT 
CGAGAGGTTC CTCTGGAGTG GCTCAGTCAC CCTTTCCGGC TGCCAGAAAA CATCATGAGG 
CCCGAGCCGG ACTACTTCAC TGCACCCTTT GACAAGAGCA AGGTGGACTT CTTCCTCATT 
GATGATAAAG ACACCTTTTT CCCACCTTCC ACACGCAACA GGATTGTGTA CTACATCCTG 
ACTCGCTGCC CATACTACAA AGAAGACCGA AAAGAGAAGG ATAAGACTGG GATTAATCGG 
CTGTTGAATA ACGGCACATA CACCTCTGCT TATCCGCTTC ATGATTGTCG GTATTGGAAG 
AAGGCTCAGG ACATGCAGTG TGAGAGTGAA AGGTATCATC TGTATCGCTA TTGGGCTCGA 
TTCCTCTGCT TCTACAAAGA GCAGCCACTC AATCTGATCA AGAAGTACTA CGGTGAGAAG 
ATCGGTATCT ACTTCGCCTG GCTGGGCTTT TACACAGAGA TGCTGTTTTA TGCTGCAGTC 
ATGGGTGTCA TCTGTTTTGT TTATGGTGTC CTCAGTTACG AAGACAACAT CACAAGTAAG 
GAAATCTGTG ACCCAAAAAT AGGAGGCATG ATTGTCATGT GTCCGCTCTG TGACAAAAAG 
TGCAGCTACT GGAAACTCAA CTCAACATGT CTGTCCTCCT GGCAATCCCA TCTCTTTGAT 
AATGAAGGAA CTGTGTTTTT TGCCATGTTC ATGGGCATTT GGGTGACCCT CTTTCTGGAG 
TTCTGGAAGC GACGGCAGGC CCGTCTGGAG TATGAGTGGG ACCTTGTGGA TTTTGAGGAG 
GAGCAGCAGC AGCTCCAGAT CAGGCCAGAG TATGAGCAGA AGTGCACAGG CCGCAGACTC 
AACCGCATCA CACAGGAAAT GGAACCTTAT CTTCCTTTCC CCAGCAAATG TGCTCGATTC 
TGCCTATCTG GAGCAACTGT ACTCTTCTGG ACGTGTCTCA TAGTGGCCTG CATAATGGGA 
GTGATCGCCT ACAGATTAGC GGTGTATGCA GCGTTCGCCA GCGTCATGAA AGACAGTTCC 
ACCAGTAAGA TCCAGCTGGT TGGGTCACTC ATCACCCCTC AGCTGGCCAC TTCCGTTACT 
GCTTCCTGCA TCAACTTCGT CATTATCCTC ATCCTCAATT TCCTCTATGA GCATGTGGCT 
ATCTGGATCA CTGACATGGA AATCCCCAAG ACCCATCTGG AGTATGAGAA CAAGCTGACC 
ATGAAGATGT TCATGTTCCA GTTTGTGAAC TACTACTCTT CCTGTTTCTA TGTGGCCTTC 
TTTAAGGGAA AGTTTGTGGG TTATCCGGGA AACTACAGTT ATATGTTTGG AAAGTGGAGC 
ACTTTGAGAA ATGAGGAGTG TGCTCCTGGA GGTTGTCTGA TTGAGCTGAC CACTCAGCTG 
CTTATTGTCA TGGCTGGGAA ACAAATGGTC GGGAACGTCC AAGAGGCTCT TTTGCCACTT 
GTCCGAAACT GGTGGAGCAG CAGGAAAGGC CGCAGTCATC CAGAAAGCAC GTATAGTAGA 
TGGGAGCAGG ACCATGACCT CCAGAACTTC AGCCAGTTTG GACTCTTCTA TGAGTATCTG 
GAGATGGTGA TCCAGTTTGG CTTCATAACA CTTTTCGTGG CCTCCTTTCC ACTGGCTCCT 
CTTCTTGCCC TCTTCAACAA CATCCTGGAG GTGCGAGTGG ATGCTTGGAA ATTCACCACA 
CAGTTCAGAC GACCGATGGC AGCCAAAGCA CGCAACATCG GAGCTTGGGA AGAGATCCTT 
AATGTGGTGG CCATCATGTC CGTGGTCACA AATGCCTTCA TCATGGCGTT CACCTCAGAT 
ATGATCCCTC GTCTGGTCTA TCTGTACGCT TATCACCCAG GCATTGAGGC CAATATGACC 
GGTTATATCA CCAACAGCCT CTCGATTTAC AACATCTCTC AGATCCCAGA AGATAACCTG 
CCTGAAGCTG GAGAAAACCC ATCCTGGTTT AACAGCTCCA CCATCACCAC ATGCAGGTAT 
CGGGACTACA GATACCCACC TGGCCATCTG AGGCAGTACA CACACACCAT GCAGTTCTGG 
CACATCCTGG CTGCTAAGCT CGCCTTCATC ATTATTATGG AACATGTTGT GTTTGTGGTA 
AAGTTCTTCG TGGCGTGGCT CATCCCAGAT GTGCCTTCAG AAGTGAAAGC CCGTATAAAG 
CGTGAGCGCT TTCTGGTCCA GGAATACCTG CACAACTATG AAGTGGAGAA ACTGAAGATG 
CAGCTAAGCC AGAGTTTCTG CCTATCCACA GAAACGGCCT CACTGCTGCC CTCTAGTCCA 
AACAAACACC AGGTGATTTC TGAATGTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168948.1
CDS	270..3062
Translation	MKRITGKTKEATLIEMTSALDSQIEDGCAVLTGYRRTLPAGNNGSINSISDRGIQLPGHREIDKFDQNRDSVFFKDGVRRIDFVLSYVDDKEGDKKAERRREFEANLEKAGLELETEDKSESDDRKTHYLKIHAPWEVLATYADVLKIKVPFKVSDIPKAREVPLEWLSHPFRLPENIMRPEPDYFTAPFDKSKVDFFLIDDKDTFFPPSTRNRIVYYILTRCPYYKEDRKEKDKTGINRLLNNGTYTSAYPLHDCRYWKKAQDMQCESERYHLYRYWARFLCFYKEQPLNLIKKYYGEKIGIYFAWLGFYTEMLFYAAVMGVICFVYGVLSYEDNITSKEICDPKIGGMIVMCPLCDKKCSYWKLNSTCLSSWQSHLFDNEGTVFFAMFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQIRPEYEQKCTGRRLNRITQEMEPYLPFPSKCARFCLSGATVLFWTCLIVACIMGVIAYRLAVYAAFASVMKDSSTSKIQLVGSLITPQLATSVTASCINFVIILILNFLYEHVAIWITDMEIPKTHLEYENKLTMKMFMFQFVNYYSSCFYVAFFKGKFVGYPGNYSYMFGKWSTLRNEECAPGGCLIELTTQLLIVMAGKQMVGNVQEALLPLVRNWWSSRKGRSHPESTYSRWEQDHDLQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNILEVRVDAWKFTTQFRRPMAAKARNIGAWEEILNVVAIMSVVTNAFIMAFTSDMIPRLVYLYAYHPGIEANMTGYITNSLSIYNISQIPEDNLPEAGENPSWFNSSTITTCRYRDYRYPPGHLRQYTHTMQFWHILAAKLAFIIIMEHVVFVVKFFVAWLIPDVPSEVKARIKRERFLVQEYLHNYEVEKLKMQLSQSFCLSTETASLLPSSPNKHQVISECV

Target ORF information:

RefSeq Version	XM_005168891.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anoctamin 5b (ano5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168891.4

ORF Insert Sequence:

ATGAAGAGGA TAACGGGCAA GACGAAAGAA GCCACTCTTA TAGAAATGAC CAGCGCGCTG 
GATTCTCAGA TTGAAGATGG CTGTGCCGTT CTGACGGGCT ACAGGAGGAC CTTACCAGCA 
GGCAATAATG GCAGCATTAA CTCAATCTCT GACCGAGGGA TTCAGCTGCC TGGTCATCGG 
GAGATAGACA AGTTTGATCA GAACAGAGAC TCTGTGTTCT TCAAGGATGG CGTACGGCGC 
ATTGACTTCG TCCTGTCCTA CGTCGATGAC AAAGAGGGAG ACAAAAAAGC GGAGAGGAGA 
AGAGAGTTTG AAGCCAACCT AGAGAAAGCT GGTCTCGAGC TGGAGACTGA AGATAAATCG 
GAGTCAGATG ATCGTAAGAC ACATTATCTG AAGATTCACG CTCCATGGGA GGTGTTGGCC 
ACTTACGCTG ATGTACTAAA GATTAAAGTG CCCTTTAAAG TGAGCGACAT CCCCAAAGCT 
CGAGAGGTTC CTCTGGAGTG GCTCAGTCAC CCTTTCCGGC TGCCAGAAAA CATCATGAGG 
CCCGAGCCGG ACTACTTCAC TGCACCCTTT GACAAGAGCA AGGTGGACTT CTTCCTCATT 
GATGATAAAG ACACCTTTTT CCCACCTTCC ACACGCAACA GGATTGTGTA CTACATCCTG 
ACTCGCTGCC CATACTACAA AGAAGACCGA AAAGAGAAGG ATAAGACTGG GATTAATCGG 
CTGTTGAATA ACGGCACATA CACCTCTGCT TATCCGCTTC ATGATTGTCG GTATTGGAAG 
AAGGCTCAGG ACATGCAGTG TGAGAGTGAA AGGTATCATC TGTATCGCTA TTGGGCTCGA 
TTCCTCTGCT TCTACAAAGA GCAGCCACTC AATCTGATCA AGAAGTACTA CGGTGAGAAG 
ATCGGTATCT ACTTCGCCTG GCTGGGCTTT TACACAGAGA TGCTGTTTTA TGCTGCAGTC 
ATGGGTGTCA TCTGTTTTGT TTATGGTGTC CTCAGTTACG AAGACAACAT CACAAGTAAG 
GAAATCTGTG ACCCAAAAAT AGGAGGCATG ATTGTCATGT GTCCGCTCTG TGACAAAAAG 
TGCAGCTACT GGAAACTCAA CTCAACATGT CTGTCCTCCT GGCAATCCCA TCTCTTTGAT 
AATGAAGGAA CTGTGTTTTT TGCCATGTTC ATGGGCATTT GGGTGACCCT CTTTCTGGAG 
TTCTGGAAGC GACGGCAGGC CCGTCTGGAG TATGAGTGGG ACCTTGTGGA TTTTGAGGAG 
GAGCAGCAGC AGCTCCAGAT CAGGCCAGAG TATGAGCAGA AGTGCACAGG CCGCAGACTC 
AACCGCATCA CACAGGAAAT GGAACCTTAT CTTCCTTTCC CCAGCAAATG TGCTCGATTC 
TGCCTATCTG GAGCAACTGT ACTCTTCTGG ACGTGTCTCA TAGTGGCCTG CATAATGGGA 
GTGATCGCCT ACAGATTAGC GGTGTATGCA GCGTTCGCCA GCGTCATGAA AGACAGTTCC 
ACCAGTAAGA TCCAGCTGGT TGGGTCACTC ATCACCCCTC AGCTGGCCAC TTCCGTTACT 
GCTTCCTGCA TCAACTTCGT CATTATCCTC ATCCTCAATT TCCTCTATGA GCATGTGGCT 
ATCTGGATCA CTGACATGGA AATCCCCAAG ACCCATCTGG AGTATGAGAA CAAGCTGACC 
ATGAAGATGT TCATGTTCCA GTTTGTGAAC TACTACTCTT CCTGTTTCTA TGTGGCCTTC 
TTTAAGGGAA AGTTTGTGGG TTATCCGGGA AACTACAGTT ATATGTTTGG AAAGTGGAGC 
ACTTTGAGAA ATGAGGAGTG TGCTCCTGGA GGTTGTCTGA TTGAGCTGAC CACTCAGCTG 
CTTATTGTCA TGGCTGGGAA ACAAATGGTC GGGAACGTCC AAGAGGCTCT TTTGCCACTT 
GTCCGAAACT GGTGGAGCAG CAGGAAAGGC CGCAGTCATC CAGAAAGCAC GTATAGTAGA 
TGGGAGCAGG ACCATGACCT CCAGAACTTC AGCCAGTTTG GACTCTTCTA TGAGTATCTG 
GAGATGGTGA TCCAGTTTGG CTTCATAACA CTTTTCGTGG CCTCCTTTCC ACTGGCTCCT 
CTTCTTGCCC TCTTCAACAA CATCCTGGAG GTGCGAGTGG ATGCTTGGAA ATTCACCACA 
CAGTTCAGAC GACCGATGGC AGCCAAAGCA CGCAACATCG GAGCTTGGGA AGAGATCCTT 
AATGTGGTGG CCATCATGTC CGTGGTCACA AATGCCTTCA TCATGGCGTT CACCTCAGAT 
ATGATCCCTC GTCTGGTCTA TCTGTACGCT TATCACCCAG GCATTGAGGC CAATATGACC 
GGTTATATCA CCAACAGCCT CTCGATTTAC AACATCTCTC AGATCCCAGA AGATAACCTG 
CCTGAAGCTG GAGAAAACCC ATCCTGGTTT AACAGCTCCA CCATCACCAC ATGCAGGTAT 
CGGGACTACA GATACCCACC TGGCCATCTG AGGCAGTACA CACACACCAT GCAGTTCTGG 
CACATCCTGG CTGCTAAGCT CGCCTTCATC ATTATTATGG AACATGTTGT GTTTGTGGTA 
AAGTTCTTCG TGGCGTGGCT CATCCCAGAT GTGCCTTCAG AAGTGAAAGC CCGTATAAAG 
CGTGAGCGCT TTCTGGTCCA GGAATACCTG CACAACTATG AAGTGGAGAA ACTGAAGATG 
CAGCTAAGCC AGAGTTTCTG CCTATCCACA GAAACGGCCT CACTGCTGCC CTCTAGTCCA 
AACAAACACC AGGTGATTTC TGAATGTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano5b ( XM_005168891.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168948.1 Danio rerio anoctamin 5b (ano5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13081
Clone ID Related Accession (Same CDS sequence)	XM_005168891.4 , XM_005168891.3
Accession Version	XM_005168891.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	anoctamin 5b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TAACGGGCAA GACGAAAGAA GCCACTCTTA TAGAAATGAC CAGCGCGCTG 
GATTCTCAGA TTGAAGATGG CTGTGCCGTT CTGACGGGCT ACAGGAGGAC CTTACCAGCA 
GGCAATAATG GCAGCATTAA CTCAATCTCT GACCGAGGGA TTCAGCTGCC TGGTCATCGG 
GAGATAGACA AGTTTGATCA GAACAGAGAC TCTGTGTTCT TCAAGGATGG CGTACGGCGC 
ATTGACTTCG TCCTGTCCTA CGTCGATGAC AAAGAGGGAG ACAAAAAAGC GGAGAGGAGA 
AGAGAGTTTG AAGCCAACCT AGAGAAAGCT GGTCTCGAGC TGGAGACTGA AGATAAATCG 
GAGTCAGATG ATCGTAAGAC ACATTATCTG AAGATTCACG CTCCATGGGA GGTGTTGGCC 
ACTTACGCTG ATGTACTAAA GATTAAAGTG CCCTTTAAAG TGAGCGACAT CCCCAAAGCT 
CGAGAGGTTC CTCTGGAGTG GCTCAGTCAC CCTTTCCGGC TGCCAGAAAA CATCATGAGG 
CCCGAGCCGG ACTACTTCAC TGCACCCTTT GACAAGAGCA AGGTGGACTT CTTCCTCATT 
GATGATAAAG ACACCTTTTT CCCACCTTCC ACACGCAACA GGATTGTGTA CTACATCCTG 
ACTCGCTGCC CATACTACAA AGAAGACCGA AAAGAGAAGG ATAAGACTGG GATTAATCGG 
CTGTTGAATA ACGGCACATA CACCTCTGCT TATCCGCTTC ATGATTGTCG GTATTGGAAG 
AAGGCTCAGG ACATGCAGTG TGAGAGTGAA AGGTATCATC TGTATCGCTA TTGGGCTCGA 
TTCCTCTGCT TCTACAAAGA GCAGCCACTC AATCTGATCA AGAAGTACTA CGGTGAGAAG 
ATCGGTATCT ACTTCGCCTG GCTGGGCTTT TACACAGAGA TGCTGTTTTA TGCTGCAGTC 
ATGGGTGTCA TCTGTTTTGT TTATGGTGTC CTCAGTTACG AAGACAACAT CACAAGTAAG 
GAAATCTGTG ACCCAAAAAT AGGAGGCATG ATTGTCATGT GTCCGCTCTG TGACAAAAAG 
TGCAGCTACT GGAAACTCAA CTCAACATGT CTGTCCTCCT GGCAATCCCA TCTCTTTGAT 
AATGAAGGAA CTGTGTTTTT TGCCATGTTC ATGGGCATTT GGGTGACCCT CTTTCTGGAG 
TTCTGGAAGC GACGGCAGGC CCGTCTGGAG TATGAGTGGG ACCTTGTGGA TTTTGAGGAG 
GAGCAGCAGC AGCTCCAGAT CAGGCCAGAG TATGAGCAGA AGTGCACAGG CCGCAGACTC 
AACCGCATCA CACAGGAAAT GGAACCTTAT CTTCCTTTCC CCAGCAAATG TGCTCGATTC 
TGCCTATCTG GAGCAACTGT ACTCTTCTGG ACGTGTCTCA TAGTGGCCTG CATAATGGGA 
GTGATCGCCT ACAGATTAGC GGTGTATGCA GCGTTCGCCA GCGTCATGAA AGACAGTTCC 
ACCAGTAAGA TCCAGCTGGT TGGGTCACTC ATCACCCCTC AGCTGGCCAC TTCCGTTACT 
GCTTCCTGCA TCAACTTCGT CATTATCCTC ATCCTCAATT TCCTCTATGA GCATGTGGCT 
ATCTGGATCA CTGACATGGA AATCCCCAAG ACCCATCTGG AGTATGAGAA CAAGCTGACC 
ATGAAGATGT TCATGTTCCA GTTTGTGAAC TACTACTCTT CCTGTTTCTA TGTGGCCTTC 
TTTAAGGGAA AGTTTGTGGG TTATCCGGGA AACTACAGTT ATATGTTTGG AAAGTGGAGC 
ACTTTGAGAA ATGAGGAGTG TGCTCCTGGA GGTTGTCTGA TTGAGCTGAC CACTCAGCTG 
CTTATTGTCA TGGCTGGGAA ACAAATGGTC GGGAACGTCC AAGAGGCTCT TTTGCCACTT 
GTCCGAAACT GGTGGAGCAG CAGGAAAGGC CGCAGTCATC CAGAAAGCAC GTATAGTAGA 
TGGGAGCAGG ACCATGACCT CCAGAACTTC AGCCAGTTTG GACTCTTCTA TGAGTATCTG 
GAGATGGTGA TCCAGTTTGG CTTCATAACA CTTTTCGTGG CCTCCTTTCC ACTGGCTCCT 
CTTCTTGCCC TCTTCAACAA CATCCTGGAG GTGCGAGTGG ATGCTTGGAA ATTCACCACA 
CAGTTCAGAC GACCGATGGC AGCCAAAGCA CGCAACATCG GAGCTTGGGA AGAGATCCTT 
AATGTGGTGG CCATCATGTC CGTGGTCACA AATGCCTTCA TCATGGCGTT CACCTCAGAT 
ATGATCCCTC GTCTGGTCTA TCTGTACGCT TATCACCCAG GCATTGAGGC CAATATGACC 
GGTTATATCA CCAACAGCCT CTCGATTTAC AACATCTCTC AGATCCCAGA AGATAACCTG 
CCTGAAGCTG GAGAAAACCC ATCCTGGTTT AACAGCTCCA CCATCACCAC ATGCAGGTAT 
CGGGACTACA GATACCCACC TGGCCATCTG AGGCAGTACA CACACACCAT GCAGTTCTGG 
CACATCCTGG CTGCTAAGCT CGCCTTCATC ATTATTATGG AACATGTTGT GTTTGTGGTA 
AAGTTCTTCG TGGCGTGGCT CATCCCAGAT GTGCCTTCAG AAGTGAAAGC CCGTATAAAG 
CGTGAGCGCT TTCTGGTCCA GGAATACCTG CACAACTATG AAGTGGAGAA ACTGAAGATG 
CAGCTAAGCC AGAGTTTCTG CCTATCCACA GAAACGGCCT CACTGCTGCC CTCTAGTCCA 
AACAAACACC AGGTGATTTC TGAATGTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168948.1
CDS	256..3048
Translation	MKRITGKTKEATLIEMTSALDSQIEDGCAVLTGYRRTLPAGNNGSINSISDRGIQLPGHREIDKFDQNRDSVFFKDGVRRIDFVLSYVDDKEGDKKAERRREFEANLEKAGLELETEDKSESDDRKTHYLKIHAPWEVLATYADVLKIKVPFKVSDIPKAREVPLEWLSHPFRLPENIMRPEPDYFTAPFDKSKVDFFLIDDKDTFFPPSTRNRIVYYILTRCPYYKEDRKEKDKTGINRLLNNGTYTSAYPLHDCRYWKKAQDMQCESERYHLYRYWARFLCFYKEQPLNLIKKYYGEKIGIYFAWLGFYTEMLFYAAVMGVICFVYGVLSYEDNITSKEICDPKIGGMIVMCPLCDKKCSYWKLNSTCLSSWQSHLFDNEGTVFFAMFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQIRPEYEQKCTGRRLNRITQEMEPYLPFPSKCARFCLSGATVLFWTCLIVACIMGVIAYRLAVYAAFASVMKDSSTSKIQLVGSLITPQLATSVTASCINFVIILILNFLYEHVAIWITDMEIPKTHLEYENKLTMKMFMFQFVNYYSSCFYVAFFKGKFVGYPGNYSYMFGKWSTLRNEECAPGGCLIELTTQLLIVMAGKQMVGNVQEALLPLVRNWWSSRKGRSHPESTYSRWEQDHDLQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNILEVRVDAWKFTTQFRRPMAAKARNIGAWEEILNVVAIMSVVTNAFIMAFTSDMIPRLVYLYAYHPGIEANMTGYITNSLSIYNISQIPEDNLPEAGENPSWFNSSTITTCRYRDYRYPPGHLRQYTHTMQFWHILAAKLAFIIIMEHVVFVVKFFVAWLIPDVPSEVKARIKRERFLVQEYLHNYEVEKLKMQLSQSFCLSTETASLLPSSPNKHQVISECV

Target ORF information:

RefSeq Version	XM_005168891.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anoctamin 5b (ano5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168891.3

ORF Insert Sequence:

ATGAAGAGGA TAACGGGCAA GACGAAAGAA GCCACTCTTA TAGAAATGAC CAGCGCGCTG 
GATTCTCAGA TTGAAGATGG CTGTGCCGTT CTGACGGGCT ACAGGAGGAC CTTACCAGCA 
GGCAATAATG GCAGCATTAA CTCAATCTCT GACCGAGGGA TTCAGCTGCC TGGTCATCGG 
GAGATAGACA AGTTTGATCA GAACAGAGAC TCTGTGTTCT TCAAGGATGG CGTACGGCGC 
ATTGACTTCG TCCTGTCCTA CGTCGATGAC AAAGAGGGAG ACAAAAAAGC GGAGAGGAGA 
AGAGAGTTTG AAGCCAACCT AGAGAAAGCT GGTCTCGAGC TGGAGACTGA AGATAAATCG 
GAGTCAGATG ATCGTAAGAC ACATTATCTG AAGATTCACG CTCCATGGGA GGTGTTGGCC 
ACTTACGCTG ATGTACTAAA GATTAAAGTG CCCTTTAAAG TGAGCGACAT CCCCAAAGCT 
CGAGAGGTTC CTCTGGAGTG GCTCAGTCAC CCTTTCCGGC TGCCAGAAAA CATCATGAGG 
CCCGAGCCGG ACTACTTCAC TGCACCCTTT GACAAGAGCA AGGTGGACTT CTTCCTCATT 
GATGATAAAG ACACCTTTTT CCCACCTTCC ACACGCAACA GGATTGTGTA CTACATCCTG 
ACTCGCTGCC CATACTACAA AGAAGACCGA AAAGAGAAGG ATAAGACTGG GATTAATCGG 
CTGTTGAATA ACGGCACATA CACCTCTGCT TATCCGCTTC ATGATTGTCG GTATTGGAAG 
AAGGCTCAGG ACATGCAGTG TGAGAGTGAA AGGTATCATC TGTATCGCTA TTGGGCTCGA 
TTCCTCTGCT TCTACAAAGA GCAGCCACTC AATCTGATCA AGAAGTACTA CGGTGAGAAG 
ATCGGTATCT ACTTCGCCTG GCTGGGCTTT TACACAGAGA TGCTGTTTTA TGCTGCAGTC 
ATGGGTGTCA TCTGTTTTGT TTATGGTGTC CTCAGTTACG AAGACAACAT CACAAGTAAG 
GAAATCTGTG ACCCAAAAAT AGGAGGCATG ATTGTCATGT GTCCGCTCTG TGACAAAAAG 
TGCAGCTACT GGAAACTCAA CTCAACATGT CTGTCCTCCT GGCAATCCCA TCTCTTTGAT 
AATGAAGGAA CTGTGTTTTT TGCCATGTTC ATGGGCATTT GGGTGACCCT CTTTCTGGAG 
TTCTGGAAGC GACGGCAGGC CCGTCTGGAG TATGAGTGGG ACCTTGTGGA TTTTGAGGAG 
GAGCAGCAGC AGCTCCAGAT CAGGCCAGAG TATGAGCAGA AGTGCACAGG CCGCAGACTC 
AACCGCATCA CACAGGAAAT GGAACCTTAT CTTCCTTTCC CCAGCAAATG TGCTCGATTC 
TGCCTATCTG GAGCAACTGT ACTCTTCTGG ACGTGTCTCA TAGTGGCCTG CATAATGGGA 
GTGATCGCCT ACAGATTAGC GGTGTATGCA GCGTTCGCCA GCGTCATGAA AGACAGTTCC 
ACCAGTAAGA TCCAGCTGGT TGGGTCACTC ATCACCCCTC AGCTGGCCAC TTCCGTTACT 
GCTTCCTGCA TCAACTTCGT CATTATCCTC ATCCTCAATT TCCTCTATGA GCATGTGGCT 
ATCTGGATCA CTGACATGGA AATCCCCAAG ACCCATCTGG AGTATGAGAA CAAGCTGACC 
ATGAAGATGT TCATGTTCCA GTTTGTGAAC TACTACTCTT CCTGTTTCTA TGTGGCCTTC 
TTTAAGGGAA AGTTTGTGGG TTATCCGGGA AACTACAGTT ATATGTTTGG AAAGTGGAGC 
ACTTTGAGAA ATGAGGAGTG TGCTCCTGGA GGTTGTCTGA TTGAGCTGAC CACTCAGCTG 
CTTATTGTCA TGGCTGGGAA ACAAATGGTC GGGAACGTCC AAGAGGCTCT TTTGCCACTT 
GTCCGAAACT GGTGGAGCAG CAGGAAAGGC CGCAGTCATC CAGAAAGCAC GTATAGTAGA 
TGGGAGCAGG ACCATGACCT CCAGAACTTC AGCCAGTTTG GACTCTTCTA TGAGTATCTG 
GAGATGGTGA TCCAGTTTGG CTTCATAACA CTTTTCGTGG CCTCCTTTCC ACTGGCTCCT 
CTTCTTGCCC TCTTCAACAA CATCCTGGAG GTGCGAGTGG ATGCTTGGAA ATTCACCACA 
CAGTTCAGAC GACCGATGGC AGCCAAAGCA CGCAACATCG GAGCTTGGGA AGAGATCCTT 
AATGTGGTGG CCATCATGTC CGTGGTCACA AATGCCTTCA TCATGGCGTT CACCTCAGAT 
ATGATCCCTC GTCTGGTCTA TCTGTACGCT TATCACCCAG GCATTGAGGC CAATATGACC 
GGTTATATCA CCAACAGCCT CTCGATTTAC AACATCTCTC AGATCCCAGA AGATAACCTG 
CCTGAAGCTG GAGAAAACCC ATCCTGGTTT AACAGCTCCA CCATCACCAC ATGCAGGTAT 
CGGGACTACA GATACCCACC TGGCCATCTG AGGCAGTACA CACACACCAT GCAGTTCTGG 
CACATCCTGG CTGCTAAGCT CGCCTTCATC ATTATTATGG AACATGTTGT GTTTGTGGTA 
AAGTTCTTCG TGGCGTGGCT CATCCCAGAT GTGCCTTCAG AAGTGAAAGC CCGTATAAAG 
CGTGAGCGCT TTCTGGTCCA GGAATACCTG CACAACTATG AAGTGGAGAA ACTGAAGATG 
CAGCTAAGCC AGAGTTTCTG CCTATCCACA GAAACGGCCT CACTGCTGCC CTCTAGTCCA 
AACAAACACC AGGTGATTTC TGAATGTGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano5b ( XM_009303225.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301500.1 Danio rerio anoctamin 5b (ano5b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43238
Clone ID Related Accession (Same CDS sequence)	XM_009303225.2 , XM_009303225.3
Accession Version	XM_009303225.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	anoctamin 5b isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303225.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGA TGATCGCTTT AACTGAGATA GACAAGTTTG ATCAGAACAG AGACTCTGTG 
TTCTTCAAGG ATGGCGTACG GCGCATTGAC TTCGTCCTGT CCTACGTCGA TGACAAAGAG 
GGAGACAAAA AAGCGGAGAG GAGAAGAGAG TTTGAAGCCA ACCTAGAGAA AGCTGGTCTC 
GAGCTGGAGA CTGAAGATAA ATCGGAGTCA GATGATCGTA AGACACATTA TCTGAAGATT 
CACGCTCCAT GGGAGGTGTT GGCCACTTAC GCTGATGTAC TAAAGATTAA AGTGCCCTTT 
AAAGTGAGCG ACATCCCCAA AGCTCGAGAG GTTCCTCTGG AGTGGCTCAG TCACCCTTTC 
CGGCTGCCAG AAAACATCAT GAGGCCCGAG CCGGACTACT TCACTGCACC CTTTGACAAG 
AGCAAGGTGG ACTTCTTCCT CATTGATGAT AAAGACACCT TTTTCCCACC TTCCACACGC 
AACAGGATTG TGTACTACAT CCTGACTCGC TGCCCATACT ACAAAGAAGA CCGAAAAGAG 
AAGGATAAGA CTGGGATTAA TCGGCTGTTG AATAACGGCA CATACACCTC TGCTTATCCG 
CTTCATGATT GTCGGTATTG GAAGAAGGCT CAGGACATGC AGTGTGAGAG TGAAAGGTAT 
CATCTGTATC GCTATTGGGC TCGATTCCTC TGCTTCTACA AAGAGCAGCC ACTCAATCTG 
ATCAAGAAGT ACTACGGTGA GAAGATCGGT ATCTACTTCG CCTGGCTGGG CTTTTACACA 
GAGATGCTGT TTTATGCTGC AGTCATGGGT GTCATCTGTT TTGTTTATGG TGTCCTCAGT 
TACGAAGACA ACATCACAAG TAAGGAAATC TGTGACCCAA AAATAGGAGG CATGATTGTC 
ATGTGTCCGC TCTGTGACAA AAAGTGCAGC TACTGGAAAC TCAACTCAAC ATGTCTGTCC 
TCCTGGCAAT CCCATCTCTT TGATAATGAA GGAACTGTGT TTTTTGCCAT GTTCATGGGC 
ATTTGGGTGA CCCTCTTTCT GGAGTTCTGG AAGCGACGGC AGGCCCGTCT GGAGTATGAG 
TGGGACCTTG TGGATTTTGA GGAGGAGCAG CAGCAGCTCC AGATCAGGCC AGAGTATGAG 
CAGAAGTGCA CAGGCCGCAG ACTCAACCGC ATCACACAGG AAATGGAACC TTATCTTCCT 
TTCCCCAGCA AATGTGCTCG ATTCTGCCTA TCTGGAGCAA CTGTACTCTT CTGGACGTGT 
CTCATAGTGG CCTGCATAAT GGGAGTGATC GCCTACAGAT TAGCGGTGTA TGCAGCGTTC 
GCCAGCGTCA TGAAAGACAG TTCCACCAGT AAGATCCAGC TGGTTGGGTC ACTCATCACC 
CCTCAGCTGG CCACTTCCGT TACTGCTTCC TGCATCAACT TCGTCATTAT CCTCATCCTC 
AATTTCCTCT ATGAGCATGT GGCTATCTGG ATCACTGACA TGGAAATCCC CAAGACCCAT 
CTGGAGTATG AGAACAAGCT GACCATGAAG ATGTTCATGT TCCAGTTTGT GAACTACTAC 
TCTTCCTGTT TCTATGTGGC CTTCTTTAAG GGAAAGTTTG TGGGTTATCC GGGAAACTAC 
AGTTATATGT TTGGAAAGTG GAGCACTTTG AGAAATGAGG AGTGTGCTCC TGGAGGTTGT 
CTGATTGAGC TGACCACTCA GCTGCTTATT GTCATGGCTG GGAAACAAAT GGTCGGGAAC 
GTCCAAGAGG CTCTTTTGCC ACTTGTCCGA AACTGGTGGA GCAGCAGGAA AGGCCGCAGT 
CATCCAGAAA GCACGTATAG TAGATGGGAG CAGGACCATG ACCTCCAGAA CTTCAGCCAG 
TTTGGACTCT TCTATGAGTA TCTGGAGATG GTGATCCAGT TTGGCTTCAT AACACTTTTC 
GTGGCCTCCT TTCCACTGGC TCCTCTTCTT GCCCTCTTCA ACAACATCCT GGAGGTGCGA 
GTGGATGCTT GGAAATTCAC CACACAGTTC AGACGACCGA TGGCAGCCAA AGCACGCAAC 
ATCGGAGCTT GGGAAGAGAT CCTTAATGTG GTGGCCATCA TGTCCGTGGT CACAAATGCC 
TTCATCATGG CGTTCACCTC AGATATGATC CCTCGTCTGG TCTATCTGTA CGCTTATCAC 
CCAGGCATTG AGGCCAATAT GACCGGTTAT ATCACCAACA GCCTCTCGAT TTACAACATC 
TCTCAGATCC CAGAAGATAA CCTGCCTGAA GCTGGAGAAA ACCCATCCTG GTTTAACAGC 
TCCACCATCA CCACATGCAG GTATCGGGAC TACAGATACC CACCTGGCCA TCTGAGGCAG 
TACACACACA CCATGCAGTT CTGGCACATC CTGGCTGCTA AGCTCGCCTT CATCATTATT 
ATGGAACATG TTGTGTTTGT GGTAAAGTTC TTCGTGGCGT GGCTCATCCC AGATGTGCCT 
TCAGAAGTGA AAGCCCGTAT AAAGCGTGAG CGCTTTCTGG TCCAGGAATA CCTGCACAAC 
TATGAAGTGG AGAAACTGAA GATGCAGCTA AGCCAGAGTT TCTGCCTATC CACAGAAACG 
GCCTCACTGC TGCCCTCTAG TCCAAACAAA CACCAGGTGA TTTCTGAATG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301500.1
CDS	65..2701
Translation	MKMMIALTEIDKFDQNRDSVFFKDGVRRIDFVLSYVDDKEGDKKAERRREFEANLEKAGLELETEDKSESDDRKTHYLKIHAPWEVLATYADVLKIKVPFKVSDIPKAREVPLEWLSHPFRLPENIMRPEPDYFTAPFDKSKVDFFLIDDKDTFFPPSTRNRIVYYILTRCPYYKEDRKEKDKTGINRLLNNGTYTSAYPLHDCRYWKKAQDMQCESERYHLYRYWARFLCFYKEQPLNLIKKYYGEKIGIYFAWLGFYTEMLFYAAVMGVICFVYGVLSYEDNITSKEICDPKIGGMIVMCPLCDKKCSYWKLNSTCLSSWQSHLFDNEGTVFFAMFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQIRPEYEQKCTGRRLNRITQEMEPYLPFPSKCARFCLSGATVLFWTCLIVACIMGVIAYRLAVYAAFASVMKDSSTSKIQLVGSLITPQLATSVTASCINFVIILILNFLYEHVAIWITDMEIPKTHLEYENKLTMKMFMFQFVNYYSSCFYVAFFKGKFVGYPGNYSYMFGKWSTLRNEECAPGGCLIELTTQLLIVMAGKQMVGNVQEALLPLVRNWWSSRKGRSHPESTYSRWEQDHDLQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNILEVRVDAWKFTTQFRRPMAAKARNIGAWEEILNVVAIMSVVTNAFIMAFTSDMIPRLVYLYAYHPGIEANMTGYITNSLSIYNISQIPEDNLPEAGENPSWFNSSTITTCRYRDYRYPPGHLRQYTHTMQFWHILAAKLAFIIIMEHVVFVVKFFVAWLIPDVPSEVKARIKRERFLVQEYLHNYEVEKLKMQLSQSFCLSTETASLLPSSPNKHQVISECV

Target ORF information:

RefSeq Version	XM_009303225.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anoctamin 5b (ano5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303225.2

ORF Insert Sequence:

ATGAAAATGA TGATCGCTTT AACTGAGATA GACAAGTTTG ATCAGAACAG AGACTCTGTG 
TTCTTCAAGG ATGGCGTACG GCGCATTGAC TTCGTCCTGT CCTACGTCGA TGACAAAGAG 
GGAGACAAAA AAGCGGAGAG GAGAAGAGAG TTTGAAGCCA ACCTAGAGAA AGCTGGTCTC 
GAGCTGGAGA CTGAAGATAA ATCGGAGTCA GATGATCGTA AGACACATTA TCTGAAGATT 
CACGCTCCAT GGGAGGTGTT GGCCACTTAC GCTGATGTAC TAAAGATTAA AGTGCCCTTT 
AAAGTGAGCG ACATCCCCAA AGCTCGAGAG GTTCCTCTGG AGTGGCTCAG TCACCCTTTC 
CGGCTGCCAG AAAACATCAT GAGGCCCGAG CCGGACTACT TCACTGCACC CTTTGACAAG 
AGCAAGGTGG ACTTCTTCCT CATTGATGAT AAAGACACCT TTTTCCCACC TTCCACACGC 
AACAGGATTG TGTACTACAT CCTGACTCGC TGCCCATACT ACAAAGAAGA CCGAAAAGAG 
AAGGATAAGA CTGGGATTAA TCGGCTGTTG AATAACGGCA CATACACCTC TGCTTATCCG 
CTTCATGATT GTCGGTATTG GAAGAAGGCT CAGGACATGC AGTGTGAGAG TGAAAGGTAT 
CATCTGTATC GCTATTGGGC TCGATTCCTC TGCTTCTACA AAGAGCAGCC ACTCAATCTG 
ATCAAGAAGT ACTACGGTGA GAAGATCGGT ATCTACTTCG CCTGGCTGGG CTTTTACACA 
GAGATGCTGT TTTATGCTGC AGTCATGGGT GTCATCTGTT TTGTTTATGG TGTCCTCAGT 
TACGAAGACA ACATCACAAG TAAGGAAATC TGTGACCCAA AAATAGGAGG CATGATTGTC 
ATGTGTCCGC TCTGTGACAA AAAGTGCAGC TACTGGAAAC TCAACTCAAC ATGTCTGTCC 
TCCTGGCAAT CCCATCTCTT TGATAATGAA GGAACTGTGT TTTTTGCCAT GTTCATGGGC 
ATTTGGGTGA CCCTCTTTCT GGAGTTCTGG AAGCGACGGC AGGCCCGTCT GGAGTATGAG 
TGGGACCTTG TGGATTTTGA GGAGGAGCAG CAGCAGCTCC AGATCAGGCC AGAGTATGAG 
CAGAAGTGCA CAGGCCGCAG ACTCAACCGC ATCACACAGG AAATGGAACC TTATCTTCCT 
TTCCCCAGCA AATGTGCTCG ATTCTGCCTA TCTGGAGCAA CTGTACTCTT CTGGACGTGT 
CTCATAGTGG CCTGCATAAT GGGAGTGATC GCCTACAGAT TAGCGGTGTA TGCAGCGTTC 
GCCAGCGTCA TGAAAGACAG TTCCACCAGT AAGATCCAGC TGGTTGGGTC ACTCATCACC 
CCTCAGCTGG CCACTTCCGT TACTGCTTCC TGCATCAACT TCGTCATTAT CCTCATCCTC 
AATTTCCTCT ATGAGCATGT GGCTATCTGG ATCACTGACA TGGAAATCCC CAAGACCCAT 
CTGGAGTATG AGAACAAGCT GACCATGAAG ATGTTCATGT TCCAGTTTGT GAACTACTAC 
TCTTCCTGTT TCTATGTGGC CTTCTTTAAG GGAAAGTTTG TGGGTTATCC GGGAAACTAC 
AGTTATATGT TTGGAAAGTG GAGCACTTTG AGAAATGAGG AGTGTGCTCC TGGAGGTTGT 
CTGATTGAGC TGACCACTCA GCTGCTTATT GTCATGGCTG GGAAACAAAT GGTCGGGAAC 
GTCCAAGAGG CTCTTTTGCC ACTTGTCCGA AACTGGTGGA GCAGCAGGAA AGGCCGCAGT 
CATCCAGAAA GCACGTATAG TAGATGGGAG CAGGACCATG ACCTCCAGAA CTTCAGCCAG 
TTTGGACTCT TCTATGAGTA TCTGGAGATG GTGATCCAGT TTGGCTTCAT AACACTTTTC 
GTGGCCTCCT TTCCACTGGC TCCTCTTCTT GCCCTCTTCA ACAACATCCT GGAGGTGCGA 
GTGGATGCTT GGAAATTCAC CACACAGTTC AGACGACCGA TGGCAGCCAA AGCACGCAAC 
ATCGGAGCTT GGGAAGAGAT CCTTAATGTG GTGGCCATCA TGTCCGTGGT CACAAATGCC 
TTCATCATGG CGTTCACCTC AGATATGATC CCTCGTCTGG TCTATCTGTA CGCTTATCAC 
CCAGGCATTG AGGCCAATAT GACCGGTTAT ATCACCAACA GCCTCTCGAT TTACAACATC 
TCTCAGATCC CAGAAGATAA CCTGCCTGAA GCTGGAGAAA ACCCATCCTG GTTTAACAGC 
TCCACCATCA CCACATGCAG GTATCGGGAC TACAGATACC CACCTGGCCA TCTGAGGCAG 
TACACACACA CCATGCAGTT CTGGCACATC CTGGCTGCTA AGCTCGCCTT CATCATTATT 
ATGGAACATG TTGTGTTTGT GGTAAAGTTC TTCGTGGCGT GGCTCATCCC AGATGTGCCT 
TCAGAAGTGA AAGCCCGTAT AAAGCGTGAG CGCTTTCTGG TCCAGGAATA CCTGCACAAC 
TATGAAGTGG AGAAACTGAA GATGCAGCTA AGCCAGAGTT TCTGCCTATC CACAGAAACG 
GCCTCACTGC TGCCCTCTAG TCCAAACAAA CACCAGGTGA TTTCTGAATG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano5b ( XM_009303225.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301500.1 Danio rerio anoctamin 5b (ano5b), transcript variant X2, mRNA.

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CloneID	ODa43238
Clone ID Related Accession (Same CDS sequence)	XM_009303225.2 , XM_009303225.3
Accession Version	XM_009303225.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	anoctamin 5b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303225.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGA TGATCGCTTT AACTGAGATA GACAAGTTTG ATCAGAACAG AGACTCTGTG 
TTCTTCAAGG ATGGCGTACG GCGCATTGAC TTCGTCCTGT CCTACGTCGA TGACAAAGAG 
GGAGACAAAA AAGCGGAGAG GAGAAGAGAG TTTGAAGCCA ACCTAGAGAA AGCTGGTCTC 
GAGCTGGAGA CTGAAGATAA ATCGGAGTCA GATGATCGTA AGACACATTA TCTGAAGATT 
CACGCTCCAT GGGAGGTGTT GGCCACTTAC GCTGATGTAC TAAAGATTAA AGTGCCCTTT 
AAAGTGAGCG ACATCCCCAA AGCTCGAGAG GTTCCTCTGG AGTGGCTCAG TCACCCTTTC 
CGGCTGCCAG AAAACATCAT GAGGCCCGAG CCGGACTACT TCACTGCACC CTTTGACAAG 
AGCAAGGTGG ACTTCTTCCT CATTGATGAT AAAGACACCT TTTTCCCACC TTCCACACGC 
AACAGGATTG TGTACTACAT CCTGACTCGC TGCCCATACT ACAAAGAAGA CCGAAAAGAG 
AAGGATAAGA CTGGGATTAA TCGGCTGTTG AATAACGGCA CATACACCTC TGCTTATCCG 
CTTCATGATT GTCGGTATTG GAAGAAGGCT CAGGACATGC AGTGTGAGAG TGAAAGGTAT 
CATCTGTATC GCTATTGGGC TCGATTCCTC TGCTTCTACA AAGAGCAGCC ACTCAATCTG 
ATCAAGAAGT ACTACGGTGA GAAGATCGGT ATCTACTTCG CCTGGCTGGG CTTTTACACA 
GAGATGCTGT TTTATGCTGC AGTCATGGGT GTCATCTGTT TTGTTTATGG TGTCCTCAGT 
TACGAAGACA ACATCACAAG TAAGGAAATC TGTGACCCAA AAATAGGAGG CATGATTGTC 
ATGTGTCCGC TCTGTGACAA AAAGTGCAGC TACTGGAAAC TCAACTCAAC ATGTCTGTCC 
TCCTGGCAAT CCCATCTCTT TGATAATGAA GGAACTGTGT TTTTTGCCAT GTTCATGGGC 
ATTTGGGTGA CCCTCTTTCT GGAGTTCTGG AAGCGACGGC AGGCCCGTCT GGAGTATGAG 
TGGGACCTTG TGGATTTTGA GGAGGAGCAG CAGCAGCTCC AGATCAGGCC AGAGTATGAG 
CAGAAGTGCA CAGGCCGCAG ACTCAACCGC ATCACACAGG AAATGGAACC TTATCTTCCT 
TTCCCCAGCA AATGTGCTCG ATTCTGCCTA TCTGGAGCAA CTGTACTCTT CTGGACGTGT 
CTCATAGTGG CCTGCATAAT GGGAGTGATC GCCTACAGAT TAGCGGTGTA TGCAGCGTTC 
GCCAGCGTCA TGAAAGACAG TTCCACCAGT AAGATCCAGC TGGTTGGGTC ACTCATCACC 
CCTCAGCTGG CCACTTCCGT TACTGCTTCC TGCATCAACT TCGTCATTAT CCTCATCCTC 
AATTTCCTCT ATGAGCATGT GGCTATCTGG ATCACTGACA TGGAAATCCC CAAGACCCAT 
CTGGAGTATG AGAACAAGCT GACCATGAAG ATGTTCATGT TCCAGTTTGT GAACTACTAC 
TCTTCCTGTT TCTATGTGGC CTTCTTTAAG GGAAAGTTTG TGGGTTATCC GGGAAACTAC 
AGTTATATGT TTGGAAAGTG GAGCACTTTG AGAAATGAGG AGTGTGCTCC TGGAGGTTGT 
CTGATTGAGC TGACCACTCA GCTGCTTATT GTCATGGCTG GGAAACAAAT GGTCGGGAAC 
GTCCAAGAGG CTCTTTTGCC ACTTGTCCGA AACTGGTGGA GCAGCAGGAA AGGCCGCAGT 
CATCCAGAAA GCACGTATAG TAGATGGGAG CAGGACCATG ACCTCCAGAA CTTCAGCCAG 
TTTGGACTCT TCTATGAGTA TCTGGAGATG GTGATCCAGT TTGGCTTCAT AACACTTTTC 
GTGGCCTCCT TTCCACTGGC TCCTCTTCTT GCCCTCTTCA ACAACATCCT GGAGGTGCGA 
GTGGATGCTT GGAAATTCAC CACACAGTTC AGACGACCGA TGGCAGCCAA AGCACGCAAC 
ATCGGAGCTT GGGAAGAGAT CCTTAATGTG GTGGCCATCA TGTCCGTGGT CACAAATGCC 
TTCATCATGG CGTTCACCTC AGATATGATC CCTCGTCTGG TCTATCTGTA CGCTTATCAC 
CCAGGCATTG AGGCCAATAT GACCGGTTAT ATCACCAACA GCCTCTCGAT TTACAACATC 
TCTCAGATCC CAGAAGATAA CCTGCCTGAA GCTGGAGAAA ACCCATCCTG GTTTAACAGC 
TCCACCATCA CCACATGCAG GTATCGGGAC TACAGATACC CACCTGGCCA TCTGAGGCAG 
TACACACACA CCATGCAGTT CTGGCACATC CTGGCTGCTA AGCTCGCCTT CATCATTATT 
ATGGAACATG TTGTGTTTGT GGTAAAGTTC TTCGTGGCGT GGCTCATCCC AGATGTGCCT 
TCAGAAGTGA AAGCCCGTAT AAAGCGTGAG CGCTTTCTGG TCCAGGAATA CCTGCACAAC 
TATGAAGTGG AGAAACTGAA GATGCAGCTA AGCCAGAGTT TCTGCCTATC CACAGAAACG 
GCCTCACTGC TGCCCTCTAG TCCAAACAAA CACCAGGTGA TTTCTGAATG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301500.1
CDS	92..2728
Translation	MKMMIALTEIDKFDQNRDSVFFKDGVRRIDFVLSYVDDKEGDKKAERRREFEANLEKAGLELETEDKSESDDRKTHYLKIHAPWEVLATYADVLKIKVPFKVSDIPKAREVPLEWLSHPFRLPENIMRPEPDYFTAPFDKSKVDFFLIDDKDTFFPPSTRNRIVYYILTRCPYYKEDRKEKDKTGINRLLNNGTYTSAYPLHDCRYWKKAQDMQCESERYHLYRYWARFLCFYKEQPLNLIKKYYGEKIGIYFAWLGFYTEMLFYAAVMGVICFVYGVLSYEDNITSKEICDPKIGGMIVMCPLCDKKCSYWKLNSTCLSSWQSHLFDNEGTVFFAMFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQIRPEYEQKCTGRRLNRITQEMEPYLPFPSKCARFCLSGATVLFWTCLIVACIMGVIAYRLAVYAAFASVMKDSSTSKIQLVGSLITPQLATSVTASCINFVIILILNFLYEHVAIWITDMEIPKTHLEYENKLTMKMFMFQFVNYYSSCFYVAFFKGKFVGYPGNYSYMFGKWSTLRNEECAPGGCLIELTTQLLIVMAGKQMVGNVQEALLPLVRNWWSSRKGRSHPESTYSRWEQDHDLQNFSQFGLFYEYLEMVIQFGFITLFVASFPLAPLLALFNNILEVRVDAWKFTTQFRRPMAAKARNIGAWEEILNVVAIMSVVTNAFIMAFTSDMIPRLVYLYAYHPGIEANMTGYITNSLSIYNISQIPEDNLPEAGENPSWFNSSTITTCRYRDYRYPPGHLRQYTHTMQFWHILAAKLAFIIIMEHVVFVVKFFVAWLIPDVPSEVKARIKRERFLVQEYLHNYEVEKLKMQLSQSFCLSTETASLLPSSPNKHQVISECV

Target ORF information:

RefSeq Version	XM_009303225.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anoctamin 5b (ano5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303225.3

ORF Insert Sequence:

ATGAAAATGA TGATCGCTTT AACTGAGATA GACAAGTTTG ATCAGAACAG AGACTCTGTG 
TTCTTCAAGG ATGGCGTACG GCGCATTGAC TTCGTCCTGT CCTACGTCGA TGACAAAGAG 
GGAGACAAAA AAGCGGAGAG GAGAAGAGAG TTTGAAGCCA ACCTAGAGAA AGCTGGTCTC 
GAGCTGGAGA CTGAAGATAA ATCGGAGTCA GATGATCGTA AGACACATTA TCTGAAGATT 
CACGCTCCAT GGGAGGTGTT GGCCACTTAC GCTGATGTAC TAAAGATTAA AGTGCCCTTT 
AAAGTGAGCG ACATCCCCAA AGCTCGAGAG GTTCCTCTGG AGTGGCTCAG TCACCCTTTC 
CGGCTGCCAG AAAACATCAT GAGGCCCGAG CCGGACTACT TCACTGCACC CTTTGACAAG 
AGCAAGGTGG ACTTCTTCCT CATTGATGAT AAAGACACCT TTTTCCCACC TTCCACACGC 
AACAGGATTG TGTACTACAT CCTGACTCGC TGCCCATACT ACAAAGAAGA CCGAAAAGAG 
AAGGATAAGA CTGGGATTAA TCGGCTGTTG AATAACGGCA CATACACCTC TGCTTATCCG 
CTTCATGATT GTCGGTATTG GAAGAAGGCT CAGGACATGC AGTGTGAGAG TGAAAGGTAT 
CATCTGTATC GCTATTGGGC TCGATTCCTC TGCTTCTACA AAGAGCAGCC ACTCAATCTG 
ATCAAGAAGT ACTACGGTGA GAAGATCGGT ATCTACTTCG CCTGGCTGGG CTTTTACACA 
GAGATGCTGT TTTATGCTGC AGTCATGGGT GTCATCTGTT TTGTTTATGG TGTCCTCAGT 
TACGAAGACA ACATCACAAG TAAGGAAATC TGTGACCCAA AAATAGGAGG CATGATTGTC 
ATGTGTCCGC TCTGTGACAA AAAGTGCAGC TACTGGAAAC TCAACTCAAC ATGTCTGTCC 
TCCTGGCAAT CCCATCTCTT TGATAATGAA GGAACTGTGT TTTTTGCCAT GTTCATGGGC 
ATTTGGGTGA CCCTCTTTCT GGAGTTCTGG AAGCGACGGC AGGCCCGTCT GGAGTATGAG 
TGGGACCTTG TGGATTTTGA GGAGGAGCAG CAGCAGCTCC AGATCAGGCC AGAGTATGAG 
CAGAAGTGCA CAGGCCGCAG ACTCAACCGC ATCACACAGG AAATGGAACC TTATCTTCCT 
TTCCCCAGCA AATGTGCTCG ATTCTGCCTA TCTGGAGCAA CTGTACTCTT CTGGACGTGT 
CTCATAGTGG CCTGCATAAT GGGAGTGATC GCCTACAGAT TAGCGGTGTA TGCAGCGTTC 
GCCAGCGTCA TGAAAGACAG TTCCACCAGT AAGATCCAGC TGGTTGGGTC ACTCATCACC 
CCTCAGCTGG CCACTTCCGT TACTGCTTCC TGCATCAACT TCGTCATTAT CCTCATCCTC 
AATTTCCTCT ATGAGCATGT GGCTATCTGG ATCACTGACA TGGAAATCCC CAAGACCCAT 
CTGGAGTATG AGAACAAGCT GACCATGAAG ATGTTCATGT TCCAGTTTGT GAACTACTAC 
TCTTCCTGTT TCTATGTGGC CTTCTTTAAG GGAAAGTTTG TGGGTTATCC GGGAAACTAC 
AGTTATATGT TTGGAAAGTG GAGCACTTTG AGAAATGAGG AGTGTGCTCC TGGAGGTTGT 
CTGATTGAGC TGACCACTCA GCTGCTTATT GTCATGGCTG GGAAACAAAT GGTCGGGAAC 
GTCCAAGAGG CTCTTTTGCC ACTTGTCCGA AACTGGTGGA GCAGCAGGAA AGGCCGCAGT 
CATCCAGAAA GCACGTATAG TAGATGGGAG CAGGACCATG ACCTCCAGAA CTTCAGCCAG 
TTTGGACTCT TCTATGAGTA TCTGGAGATG GTGATCCAGT TTGGCTTCAT AACACTTTTC 
GTGGCCTCCT TTCCACTGGC TCCTCTTCTT GCCCTCTTCA ACAACATCCT GGAGGTGCGA 
GTGGATGCTT GGAAATTCAC CACACAGTTC AGACGACCGA TGGCAGCCAA AGCACGCAAC 
ATCGGAGCTT GGGAAGAGAT CCTTAATGTG GTGGCCATCA TGTCCGTGGT CACAAATGCC 
TTCATCATGG CGTTCACCTC AGATATGATC CCTCGTCTGG TCTATCTGTA CGCTTATCAC 
CCAGGCATTG AGGCCAATAT GACCGGTTAT ATCACCAACA GCCTCTCGAT TTACAACATC 
TCTCAGATCC CAGAAGATAA CCTGCCTGAA GCTGGAGAAA ACCCATCCTG GTTTAACAGC 
TCCACCATCA CCACATGCAG GTATCGGGAC TACAGATACC CACCTGGCCA TCTGAGGCAG 
TACACACACA CCATGCAGTT CTGGCACATC CTGGCTGCTA AGCTCGCCTT CATCATTATT 
ATGGAACATG TTGTGTTTGT GGTAAAGTTC TTCGTGGCGT GGCTCATCCC AGATGTGCCT 
TCAGAAGTGA AAGCCCGTAT AAAGCGTGAG CGCTTTCTGG TCCAGGAATA CCTGCACAAC 
TATGAAGTGG AGAAACTGAA GATGCAGCTA AGCCAGAGTT TCTGCCTATC CACAGAAACG 
GCCTCACTGC TGCCCTCTAG TCCAAACAAA CACCAGGTGA TTTCTGAATG TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Comparative genomic analysis of teleost fish bmal genes.
Genetica136(1)149-61(2009 May)
Wang H

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ano5b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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