acly-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	acly-1
Entrez Gene ID	180485
Full Name	Probable ATP-citrate synthase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_075879.5	NP_508280.1	Probable ATP-citrate synthase
NM_075879.4	NP_508280.1	Probable ATP-citrate synthase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Acly	NP_001186225.1
Gallus gallus (chicken)	ACLY	NP_001025711.1
Danio rerio (zebrafish)	aclya	NP_001002649.1
Caenorhabditis elegans (roundworm)	acly-2	NP_506267.1
Magnaporthe oryzae (rice blast fungus)	MGG_06719	XP_003709430.1
Arabidopsis thaliana (thale cress)	ACLB-2	NP_199757.1
Arabidopsis thaliana (thale cress)	ACLB-1	NP_187317.1
Caenorhabditis elegans (roundworm)	D1005.1	NP_508280.1
Rattus norvegicus (Norway rat)	Acly	NP_058683.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010156	XP_319323.3
Oryza sativa (rice)	Os01g0300200	NP_001042817.1
Xenopus tropicalis (tropical clawed frog)	acly	NP_001008028.1
	ACLY	XP_001108114.2
Bos taurus (cattle)	ACLY	NP_001032534.1
Homo sapiens (human)	ACLY	NP_001087.2
Pan troglodytes (chimpanzee)	ACLY	XP_511495.3
Canis lupus familiaris (dog)	ACLY	XP_850198.1
Drosophila melanogaster (fruit fly)	ATPCL	NP_725514.2
Schizosaccharomyces pombe (fission yeast)	SPBC1703.07	NP_596202.1
Neurospora crassa	NCU06785	XP_963626.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following acly-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acly-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa349627	NM_075879.5 Latest version!	Caenorhabditis elegans Probable ATP-citrate synthase (acly-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa349627	NM_075879.4	Caenorhabditis elegans Probable ATP-citrate synthase (acly-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

acly-1 ( NM_075879.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_508280.1 Caenorhabditis elegans Probable ATP-citrate synthase (acly-1), mRNA.

Price & Availability↑

CloneID	OCa349627
Clone ID Related Accession (Same CDS sequence)	NM_075879.5 , NM_075879.4
Accession Version	NM_075879.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Probable ATP-citrate synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_075879.4.

ATGTCGGCGA AGGCTGTGAG CGAGTTATCT GGAAAAGAAG TTCTCTACAA ATACTTCGAG 
CCAAGCGGAC TCCTCTCAGC TCCACATGCG TTTCACGTGA AGGCTGGCGA AAATTTTGAC 
GAAATTGCAA ACAAGTACGA GTGGCTCGCA CGTGATAACA AGGGAGTCAT CAAGCCCGAC 
CAGTTGATCA AAAGACGTGG AAAGCTGGGT TTGGTCAAGA TTGGAACACC ACAAGAGTTG 
AAGGCATGGT TCGAGAAAAC TGGTGACAGT TATGTGAGAG TTGGTCAAAC CGAGGGACGG 
CTTCACACTT TTATTGTCGA GCCATTTTGT GCACACACTG AAAAGGATGA GATGTACATT 
GCGATCTACA GTGAGAGATT TCGTGACGTC ATCATGTTCT ATGAGCAAGG AGGTGTAGAT 
ATTGGAGATG TTGAGGAAAA GGCTCGCACT GTATCCGTCC CCGTCCAGCT CAACGAGAAT 
GCCATGACCC CCTCGGATGA AGAGCTCACC ACCCTCCTGG GCCCACTCAA GGACAGTGAC 
ATTGTCAGAA GATTTGTGGT AGAGCTCTAC AAAGCCTACA AGGACCTACA TTTCACGTAT 
CTCGAGATTA ATCCATTTGT CCTTTTGAAC AACCAGATTC ATGTTTTGGA TTTGGCAGCT 
AGGCTTGATG AAACCGCCAA TTTCTTGTGC GCAGACAAGT GGAAGAGCCG TCTGACACCA 
TATGGAGGAC CGAACCACGT GGAGTTCCCA GCTCCGTTTG GACGTGATTT GACGTCAGAG 
GAGCAGTACA TTTCGGAGAT GGATGCGAAG ACGGGAGCTT CCTTGAAGCT GACGATTTTG 
AACAGAAAGG GAAGAGTTTG GACGATGGTG GCTGGTGGTG GAGCTTCAGT TGTGTTTACC 
GACACTGTCT GTGACCTCGG TGGAGCATCA GAACTCGCCA ACTACGGAGA ATACTCTGGA 
GACCCGTCTG AATCGCAAAC CTATGAATAC GCAAAGACAC TCCTCTCCGT GATGACCGAA 
GGTACTCCGA GACCTGATGG AAAAGTCCTC ATCATCGGAG GATCCATCGC CAACTTCACA 
AATGTTGCTA AAACTTTTGG TGGAATCGTC AGAGCCTTTG AAACGTTTGT CTCAAAGCTC 
AAAGAGCACA AGGTGACAAT TTTCGTGCGT CGCGGAGGCC CAAACTACCA GGAGGGACTC 
AGAAGAATCA AGGATGCTGC GACAAAGTTG GAGCTGCCAA TTCATGTATT CGGACCTGAG 
ACTCATATGA CGGCGATTGT TGGAGCAGCG CTCGGAGTGA AGCCAATGCC AACAGTTCCG 
ACAGCTCCAC AGACTACCGG ACAGTTTTTG TTGAGCCCTG AGAGAAATAC CGGAGGCACT 
GAGAGAGCTC CACCTTCGCC AGCAGCCAAC GCCACTCCAA CTGAGCACCC ACTCACCACT 
GCCCAACAGA ACAAACTGAA AAGCTTCCGT GGACTCTTCG AGGACGACAC CAAGGCCATC 
ATCTGGGGAC AACAGGCTAA GGCTATTCAA GGAATGCTGG ACTTTGACTA TGTCTGCAGA 
AGAAGCTCCC CGTCGGTTGT CGCGTCCACC TACCCGTTCA CTGGGGACAA CAAGCAGAAG 
TACTACTTTG GGCAAAAGGA AATTCTCATT CCAGCCTATA AGTCGATGGC GAAGGCTTTT 
GCAACTCATC CGGATGCTTC TATCATGGTC ACTTTTGCCT CGATGAGATC TGTATTTGAG 
ACTGTTCTCG AGGCGCTCGA GTTCCCACAA ATCAAGGTTA TTGCGATCAT TGCTGAAGGT 
GTCCCTGAGA ATCAGACTAG AAAGCTGTTG AAGATTGCTC ATGACCGTGG AGTCACCCTT 
GTTGGGCCAG CCACCGTCGG AGGTATCAAG CCGGGATGCT TTAAGATTGG AAACACCGGT 
GGAATGATGG ACAACATCCT TGCCTCAAAG CTCTACCGCC CTGGAAGTGT CGCCTATGTA 
TCTCGCTCCG GAGGAATGTC CAATGAGCTT AACAATATCA TTTCACAAAA CACTAACGGA 
GTCTATGAAG GTATTGCAAT TGGAGGTGAC CGCTATCCGG GAAGCACGTA CACTGATCAC 
GTGATTCGCT ATCAAAATGA TGATCGTGTG AAGATGATTG TTCTTCTCGG TGAGGTCGGT 
GGAGTTGAAG AGTACAAAAT TGTGGATCTC CTCAAGCAGA AGAAGGTGAC AAAGCCATTG 
GTCGCATGGT GCATCGGAAC CTGTGCAGAT CACATCACAT CGGAGGTTCA ATTCGGTCAC 
GCTGGAGCAT CTGCAAATGC TCTCGGAGAA ACCGCTGCCT GCAAAAATGC TGCTCTCCGT 
GCTTCAGGTG CCCTTGTCCC CGAGTCATTT GACGATCTTG GCAACAAAAT CCGTCAGACG 
TACGACGAAC TTGTCAGCCA ACAAATCATC GTTCCACAGC CAGAAGTTCC ACCTCCAGCG 
GTGCCGATGG ATTACGCGTG GGCTCGTGAA CTGGGACTCA TTAGAAAACC AGCTTCCTTC 
ATGACCTCAA TTTGTGATGA GAGAGGAGAA GAGTTGAACT ATGCGGGAGT ACCGATTACA 
AAGGTTTTGG AGTCGGATAT GGGAATCGGT GGAGTGTTGG GATTGTTGTG GTTCCAGAAG 
CGTCTCCCAC CACATGCCAA CAAATTTATC GAGATCTGCT TGATGCTCAC CGCCGATCAC 
GGACCAGCTG TGTCGGGAGC CCATAACACT ATTGTGTGTG CTCGTGCTGG AAAAGATCTG 
ATCTCTTCGC TCACGTCTGG TCTACTCACC ATTGGTGATC GCTTCGGAGG TGCCCTCGAT 
GGAGCCGCTC GTCAGTTCTC TGAGGCTTTT GATCAAGGAT GGTCGGCGAA TCAGTTTGTC 
AGCGAGATGA GAAAGAAGGG AAAGCATATT ATGGGAATTG GACATCGTGT GAAGAGTATC 
AACAACCCTG ACAAACGTGT GGAGATTCTG AAAAGATTTG CCATGGACAA GAAGGAGTTT 
GCCCAGGAGA CGCCGTTGTT CGAGTATGCT CTTGAGGTCG AGAAGATCAC GACCGCCAAA 
AAACCGAACC TCATTCTCAA CGTGGACGGA GCGATTGCGA TCTTATTCGT CGATATCCTT 
CGCCACAGTG GCATGTTCAC CAAGCAGGAG GCCGAGGAGA CAATCGAGAT TGGATCGCTC 
AATGGGCTTT TCGTTTTGGG TAGATCTATT GGATTTATCG GTCACTACTT GGATCAGTCG 
CGTCTGAAGC AGGGATTGTA CCGTCATCCA TGGGATGATA TTTCCTACAT TATGCCGGAG 
AGCAACTTGG TAAAGTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_508280.1
CDS	31..3351
Translation	MSAKAVSELSGKEVLYKYFEPSGLLSAPHAFHVKAGENFDEIANKYEWLARDNKGVIKPDQLIKRRGKLGLVKIGTPQELKAWFEKTGDSYVRVGQTEGRLHTFIVEPFCAHTEKDEMYIAIYSERFRDVIMFYEQGGVDIGDVEEKARTVSVPVQLNENAMTPSDEELTTLLGPLKDSDIVRRFVVELYKAYKDLHFTYLEINPFVLLNNQIHVLDLAARLDETANFLCADKWKSRLTPYGGPNHVEFPAPFGRDLTSEEQYISEMDAKTGASLKLTILNRKGRVWTMVAGGGASVVFTDTVCDLGGASELANYGEYSGDPSESQTYEYAKTLLSVMTEGTPRPDGKVLIIGGSIANFTNVAKTFGGIVRAFETFVSKLKEHKVTIFVRRGGPNYQEGLRRIKDAATKLELPIHVFGPETHMTAIVGAALGVKPMPTVPTAPQTTGQFLLSPERNTGGTERAPPSPAANATPTEHPLTTAQQNKLKSFRGLFEDDTKAIIWGQQAKAIQGMLDFDYVCRRSSPSVVASTYPFTGDNKQKYYFGQKEILIPAYKSMAKAFATHPDASIMVTFASMRSVFETVLEALEFPQIKVIAIIAEGVPENQTRKLLKIAHDRGVTLVGPATVGGIKPGCFKIGNTGGMMDNILASKLYRPGSVAYVSRSGGMSNELNNIISQNTNGVYEGIAIGGDRYPGSTYTDHVIRYQNDDRVKMIVLLGEVGGVEEYKIVDLLKQKKVTKPLVAWCIGTCADHITSEVQFGHAGASANALGETAACKNAALRASGALVPESFDDLGNKIRQTYDELVSQQIIVPQPEVPPPAVPMDYAWARELGLIRKPASFMTSICDERGEELNYAGVPITKVLESDMGIGGVLGLLWFQKRLPPHANKFIEICLMLTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDGAARQFSEAFDQGWSANQFVSEMRKKGKHIMGIGHRVKSINNPDKRVEILKRFAMDKKEFAQETPLFEYALEVEKITTAKKPNLILNVDGAIAILFVDILRHSGMFTKQEAEETIEIGSLNGLFVLGRSIGFIGHYLDQSRLKQGLYRHPWDDISYIMPESNLVKF

Target ORF information:

RefSeq Version	NM_075879.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Probable ATP-citrate synthase (acly-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_075879.5

ORF Insert Sequence:

ATGTCGGCGA AGGCTGTGAG CGAGTTATCT GGAAAAGAAG TTCTCTACAA ATACTTCGAG 
CCAAGCGGAC TCCTCTCAGC TCCACATGCG TTTCACGTGA AGGCTGGCGA AAATTTTGAC 
GAAATTGCAA ACAAGTACGA GTGGCTCGCA CGTGATAACA AGGGAGTCAT CAAGCCCGAC 
CAGTTGATCA AAAGACGTGG AAAGCTGGGT TTGGTCAAGA TTGGAACACC ACAAGAGTTG 
AAGGCATGGT TCGAGAAAAC TGGTGACAGT TATGTGAGAG TTGGTCAAAC CGAGGGACGG 
CTTCACACTT TTATTGTCGA GCCATTTTGT GCACACACTG AAAAGGATGA GATGTACATT 
GCGATCTACA GTGAGAGATT TCGTGACGTC ATCATGTTCT ATGAGCAAGG AGGTGTAGAT 
ATTGGAGATG TTGAGGAAAA GGCTCGCACT GTATCCGTCC CCGTCCAGCT CAACGAGAAT 
GCCATGACCC CCTCGGATGA AGAGCTCACC ACCCTCCTGG GCCCACTCAA GGACAGTGAC 
ATTGTCAGAA GATTTGTGGT AGAGCTCTAC AAAGCCTACA AGGACCTACA TTTCACGTAT 
CTCGAGATTA ATCCATTTGT CCTTTTGAAC AACCAGATTC ATGTTTTGGA TTTGGCAGCT 
AGGCTTGATG AAACCGCCAA TTTCTTGTGC GCAGACAAGT GGAAGAGCCG TCTGACACCA 
TATGGAGGAC CGAACCACGT GGAGTTCCCA GCTCCGTTTG GACGTGATTT GACGTCAGAG 
GAGCAGTACA TTTCGGAGAT GGATGCGAAG ACGGGAGCTT CCTTGAAGCT GACGATTTTG 
AACAGAAAGG GAAGAGTTTG GACGATGGTG GCTGGTGGTG GAGCTTCAGT TGTGTTTACC 
GACACTGTCT GTGACCTCGG TGGAGCATCA GAACTCGCCA ACTACGGAGA ATACTCTGGA 
GACCCGTCTG AATCGCAAAC CTATGAATAC GCAAAGACAC TCCTCTCCGT GATGACCGAA 
GGTACTCCGA GACCTGATGG AAAAGTCCTC ATCATCGGAG GATCCATCGC CAACTTCACA 
AATGTTGCTA AAACTTTTGG TGGAATCGTC AGAGCCTTTG AAACGTTTGT CTCAAAGCTC 
AAAGAGCACA AGGTGACAAT TTTCGTGCGT CGCGGAGGCC CAAACTACCA GGAGGGACTC 
AGAAGAATCA AGGATGCTGC GACAAAGTTG GAGCTGCCAA TTCATGTATT CGGACCTGAG 
ACTCATATGA CGGCGATTGT TGGAGCAGCG CTCGGAGTGA AGCCAATGCC AACAGTTCCG 
ACAGCTCCAC AGACTACCGG ACAGTTTTTG TTGAGCCCTG AGAGAAATAC CGGAGGCACT 
GAGAGAGCTC CACCTTCGCC AGCAGCCAAC GCCACTCCAA CTGAGCACCC ACTCACCACT 
GCCCAACAGA ACAAACTGAA AAGCTTCCGT GGACTCTTCG AGGACGACAC CAAGGCCATC 
ATCTGGGGAC AACAGGCTAA GGCTATTCAA GGAATGCTGG ACTTTGACTA TGTCTGCAGA 
AGAAGCTCCC CGTCGGTTGT CGCGTCCACC TACCCGTTCA CTGGGGACAA CAAGCAGAAG 
TACTACTTTG GGCAAAAGGA AATTCTCATT CCAGCCTATA AGTCGATGGC GAAGGCTTTT 
GCAACTCATC CGGATGCTTC TATCATGGTC ACTTTTGCCT CGATGAGATC TGTATTTGAG 
ACTGTTCTCG AGGCGCTCGA GTTCCCACAA ATCAAGGTTA TTGCGATCAT TGCTGAAGGT 
GTCCCTGAGA ATCAGACTAG AAAGCTGTTG AAGATTGCTC ATGACCGTGG AGTCACCCTT 
GTTGGGCCAG CCACCGTCGG AGGTATCAAG CCGGGATGCT TTAAGATTGG AAACACCGGT 
GGAATGATGG ACAACATCCT TGCCTCAAAG CTCTACCGCC CTGGAAGTGT CGCCTATGTA 
TCTCGCTCCG GAGGAATGTC CAATGAGCTT AACAATATCA TTTCACAAAA CACTAACGGA 
GTCTATGAAG GTATTGCAAT TGGAGGTGAC CGCTATCCGG GAAGCACGTA CACTGATCAC 
GTGATTCGCT ATCAAAATGA TGATCGTGTG AAGATGATTG TTCTTCTCGG TGAGGTCGGT 
GGAGTTGAAG AGTACAAAAT TGTGGATCTC CTCAAGCAGA AGAAGGTGAC AAAGCCATTG 
GTCGCATGGT GCATCGGAAC CTGTGCAGAT CACATCACAT CGGAGGTTCA ATTCGGTCAC 
GCTGGAGCAT CTGCAAATGC TCTCGGAGAA ACCGCTGCCT GCAAAAATGC TGCTCTCCGT 
GCTTCAGGTG CCCTTGTCCC CGAGTCATTT GACGATCTTG GCAACAAAAT CCGTCAGACG 
TACGACGAAC TTGTCAGCCA ACAAATCATC GTTCCACAGC CAGAAGTTCC ACCTCCAGCG 
GTGCCGATGG ATTACGCGTG GGCTCGTGAA CTGGGACTCA TTAGAAAACC AGCTTCCTTC 
ATGACCTCAA TTTGTGATGA GAGAGGAGAA GAGTTGAACT ATGCGGGAGT ACCGATTACA 
AAGGTTTTGG AGTCGGATAT GGGAATCGGT GGAGTGTTGG GATTGTTGTG GTTCCAGAAG 
CGTCTCCCAC CACATGCCAA CAAATTTATC GAGATCTGCT TGATGCTCAC CGCCGATCAC 
GGACCAGCTG TGTCGGGAGC CCATAACACT ATTGTGTGTG CTCGTGCTGG AAAAGATCTG 
ATCTCTTCGC TCACGTCTGG TCTACTCACC ATTGGTGATC GCTTCGGAGG TGCCCTCGAT 
GGAGCCGCTC GTCAGTTCTC TGAGGCTTTT GATCAAGGAT GGTCGGCGAA TCAGTTTGTC 
AGCGAGATGA GAAAGAAGGG AAAGCATATT ATGGGAATTG GACATCGTGT GAAGAGTATC 
AACAACCCTG ACAAACGTGT GGAGATTCTG AAAAGATTTG CCATGGACAA GAAGGAGTTT 
GCCCAGGAGA CGCCGTTGTT CGAGTATGCT CTTGAGGTCG AGAAGATCAC GACCGCCAAA 
AAACCGAACC TCATTCTCAA CGTGGACGGA GCGATTGCGA TCTTATTCGT CGATATCCTT 
CGCCACAGTG GCATGTTCAC CAAGCAGGAG GCCGAGGAGA CAATCGAGAT TGGATCGCTC 
AATGGGCTTT TCGTTTTGGG TAGATCTATT GGATTTATCG GTCACTACTT GGATCAGTCG 
CGTCTGAAGC AGGGATTGTA CCGTCATCCA TGGGATGATA TTTCCTACAT TATGCCGGAG 
AGCAACTTGG TAAAGTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acly-1 ( NM_075879.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_508280.1 Caenorhabditis elegans Probable ATP-citrate synthase (acly-1), partial mRNA.

Price & Availability↑

CloneID	OCa349627
Clone ID Related Accession (Same CDS sequence)	NM_075879.5 , NM_075879.4
Accession Version	NM_075879.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Probable ATP-citrate synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_075879.3. COMPLETENESS: incomplete on both ends.

ATGTCGGCGA AGGCTGTGAG CGAGTTATCT GGAAAAGAAG TTCTCTACAA ATACTTCGAG 
CCAAGCGGAC TCCTCTCAGC TCCACATGCG TTTCACGTGA AGGCTGGCGA AAATTTTGAC 
GAAATTGCAA ACAAGTACGA GTGGCTCGCA CGTGATAACA AGGGAGTCAT CAAGCCCGAC 
CAGTTGATCA AAAGACGTGG AAAGCTGGGT TTGGTCAAGA TTGGAACACC ACAAGAGTTG 
AAGGCATGGT TCGAGAAAAC TGGTGACAGT TATGTGAGAG TTGGTCAAAC CGAGGGACGG 
CTTCACACTT TTATTGTCGA GCCATTTTGT GCACACACTG AAAAGGATGA GATGTACATT 
GCGATCTACA GTGAGAGATT TCGTGACGTC ATCATGTTCT ATGAGCAAGG AGGTGTAGAT 
ATTGGAGATG TTGAGGAAAA GGCTCGCACT GTATCCGTCC CCGTCCAGCT CAACGAGAAT 
GCCATGACCC CCTCGGATGA AGAGCTCACC ACCCTCCTGG GCCCACTCAA GGACAGTGAC 
ATTGTCAGAA GATTTGTGGT AGAGCTCTAC AAAGCCTACA AGGACCTACA TTTCACGTAT 
CTCGAGATTA ATCCATTTGT CCTTTTGAAC AACCAGATTC ATGTTTTGGA TTTGGCAGCT 
AGGCTTGATG AAACCGCCAA TTTCTTGTGC GCAGACAAGT GGAAGAGCCG TCTGACACCA 
TATGGAGGAC CGAACCACGT GGAGTTCCCA GCTCCGTTTG GACGTGATTT GACGTCAGAG 
GAGCAGTACA TTTCGGAGAT GGATGCGAAG ACGGGAGCTT CCTTGAAGCT GACGATTTTG 
AACAGAAAGG GAAGAGTTTG GACGATGGTG GCTGGTGGTG GAGCTTCAGT TGTGTTTACC 
GACACTGTCT GTGACCTCGG TGGAGCATCA GAACTCGCCA ACTACGGAGA ATACTCTGGA 
GACCCGTCTG AATCGCAAAC CTATGAATAC GCAAAGACAC TCCTCTCCGT GATGACCGAA 
GGTACTCCGA GACCTGATGG AAAAGTCCTC ATCATCGGAG GATCCATCGC CAACTTCACA 
AATGTTGCTA AAACTTTTGG TGGAATCGTC AGAGCCTTTG AAACGTTTGT CTCAAAGCTC 
AAAGAGCACA AGGTGACAAT TTTCGTGCGT CGCGGAGGCC CAAACTACCA GGAGGGACTC 
AGAAGAATCA AGGATGCTGC GACAAAGTTG GAGCTGCCAA TTCATGTATT CGGACCTGAG 
ACTCATATGA CGGCGATTGT TGGAGCAGCG CTCGGAGTGA AGCCAATGCC AACAGTTCCG 
ACAGCTCCAC AGACTACCGG ACAGTTTTTG TTGAGCCCTG AGAGAAATAC CGGAGGCACT 
GAGAGAGCTC CACCTTCGCC AGCAGCCAAC GCCACTCCAA CTGAGCACCC ACTCACCACT 
GCCCAACAGA ACAAACTGAA AAGCTTCCGT GGACTCTTCG AGGACGACAC CAAGGCCATC 
ATCTGGGGAC AACAGGCTAA GGCTATTCAA GGAATGCTGG ACTTTGACTA TGTCTGCAGA 
AGAAGCTCCC CGTCGGTTGT CGCGTCCACC TACCCGTTCA CTGGGGACAA CAAGCAGAAG 
TACTACTTTG GGCAAAAGGA AATTCTCATT CCAGCCTATA AGTCGATGGC GAAGGCTTTT 
GCAACTCATC CGGATGCTTC TATCATGGTC ACTTTTGCCT CGATGAGATC TGTATTTGAG 
ACTGTTCTCG AGGCGCTCGA GTTCCCACAA ATCAAGGTTA TTGCGATCAT TGCTGAAGGT 
GTCCCTGAGA ATCAGACTAG AAAGCTGTTG AAGATTGCTC ATGACCGTGG AGTCACCCTT 
GTTGGGCCAG CCACCGTCGG AGGTATCAAG CCGGGATGCT TTAAGATTGG AAACACCGGT 
GGAATGATGG ACAACATCCT TGCCTCAAAG CTCTACCGCC CTGGAAGTGT CGCCTATGTA 
TCTCGCTCCG GAGGAATGTC CAATGAGCTT AACAATATCA TTTCACAAAA CACTAACGGA 
GTCTATGAAG GTATTGCAAT TGGAGGTGAC CGCTATCCGG GAAGCACGTA CACTGATCAC 
GTGATTCGCT ATCAAAATGA TGATCGTGTG AAGATGATTG TTCTTCTCGG TGAGGTCGGT 
GGAGTTGAAG AGTACAAAAT TGTGGATCTC CTCAAGCAGA AGAAGGTGAC AAAGCCATTG 
GTCGCATGGT GCATCGGAAC CTGTGCAGAT CACATCACAT CGGAGGTTCA ATTCGGTCAC 
GCTGGAGCAT CTGCAAATGC TCTCGGAGAA ACCGCTGCCT GCAAAAATGC TGCTCTCCGT 
GCTTCAGGTG CCCTTGTCCC CGAGTCATTT GACGATCTTG GCAACAAAAT CCGTCAGACG 
TACGACGAAC TTGTCAGCCA ACAAATCATC GTTCCACAGC CAGAAGTTCC ACCTCCAGCG 
GTGCCGATGG ATTACGCGTG GGCTCGTGAA CTGGGACTCA TTAGAAAACC AGCTTCCTTC 
ATGACCTCAA TTTGTGATGA GAGAGGAGAA GAGTTGAACT ATGCGGGAGT ACCGATTACA 
AAGGTTTTGG AGTCGGATAT GGGAATCGGT GGAGTGTTGG GATTGTTGTG GTTCCAGAAG 
CGTCTCCCAC CACATGCCAA CAAATTTATC GAGATCTGCT TGATGCTCAC CGCCGATCAC 
GGACCAGCTG TGTCGGGAGC CCATAACACT ATTGTGTGTG CTCGTGCTGG AAAAGATCTG 
ATCTCTTCGC TCACGTCTGG TCTACTCACC ATTGGTGATC GCTTCGGAGG TGCCCTCGAT 
GGAGCCGCTC GTCAGTTCTC TGAGGCTTTT GATCAAGGAT GGTCGGCGAA TCAGTTTGTC 
AGCGAGATGA GAAAGAAGGG AAAGCATATT ATGGGAATTG GACATCGTGT GAAGAGTATC 
AACAACCCTG ACAAACGTGT GGAGATTCTG AAAAGATTTG CCATGGACAA GAAGGAGTTT 
GCCCAGGAGA CGCCGTTGTT CGAGTATGCT CTTGAGGTCG AGAAGATCAC GACCGCCAAA 
AAACCGAACC TCATTCTCAA CGTGGACGGA GCGATTGCGA TCTTATTCGT CGATATCCTT 
CGCCACAGTG GCATGTTCAC CAAGCAGGAG GCCGAGGAGA CAATCGAGAT TGGATCGCTC 
AATGGGCTTT TCGTTTTGGG TAGATCTATT GGATTTATCG GTCACTACTT GGATCAGTCG 
CGTCTGAAGC AGGGATTGTA CCGTCATCCA TGGGATGATA TTTCCTACAT TATGCCGGAG 
AGCAACTTGG TAAAGTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_508280.1
CDS	1..3321
Translation	MSAKAVSELSGKEVLYKYFEPSGLLSAPHAFHVKAGENFDEIANKYEWLARDNKGVIKPDQLIKRRGKLGLVKIGTPQELKAWFEKTGDSYVRVGQTEGRLHTFIVEPFCAHTEKDEMYIAIYSERFRDVIMFYEQGGVDIGDVEEKARTVSVPVQLNENAMTPSDEELTTLLGPLKDSDIVRRFVVELYKAYKDLHFTYLEINPFVLLNNQIHVLDLAARLDETANFLCADKWKSRLTPYGGPNHVEFPAPFGRDLTSEEQYISEMDAKTGASLKLTILNRKGRVWTMVAGGGASVVFTDTVCDLGGASELANYGEYSGDPSESQTYEYAKTLLSVMTEGTPRPDGKVLIIGGSIANFTNVAKTFGGIVRAFETFVSKLKEHKVTIFVRRGGPNYQEGLRRIKDAATKLELPIHVFGPETHMTAIVGAALGVKPMPTVPTAPQTTGQFLLSPERNTGGTERAPPSPAANATPTEHPLTTAQQNKLKSFRGLFEDDTKAIIWGQQAKAIQGMLDFDYVCRRSSPSVVASTYPFTGDNKQKYYFGQKEILIPAYKSMAKAFATHPDASIMVTFASMRSVFETVLEALEFPQIKVIAIIAEGVPENQTRKLLKIAHDRGVTLVGPATVGGIKPGCFKIGNTGGMMDNILASKLYRPGSVAYVSRSGGMSNELNNIISQNTNGVYEGIAIGGDRYPGSTYTDHVIRYQNDDRVKMIVLLGEVGGVEEYKIVDLLKQKKVTKPLVAWCIGTCADHITSEVQFGHAGASANALGETAACKNAALRASGALVPESFDDLGNKIRQTYDELVSQQIIVPQPEVPPPAVPMDYAWARELGLIRKPASFMTSICDERGEELNYAGVPITKVLESDMGIGGVLGLLWFQKRLPPHANKFIEICLMLTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDGAARQFSEAFDQGWSANQFVSEMRKKGKHIMGIGHRVKSINNPDKRVEILKRFAMDKKEFAQETPLFEYALEVEKITTAKKPNLILNVDGAIAILFVDILRHSGMFTKQEAEETIEIGSLNGLFVLGRSIGFIGHYLDQSRLKQGLYRHPWDDISYIMPESNLVKF

Target ORF information:

RefSeq Version	NM_075879.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Probable ATP-citrate synthase (acly-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_075879.4

ORF Insert Sequence:

ATGTCGGCGA AGGCTGTGAG CGAGTTATCT GGAAAAGAAG TTCTCTACAA ATACTTCGAG 
CCAAGCGGAC TCCTCTCAGC TCCACATGCG TTTCACGTGA AGGCTGGCGA AAATTTTGAC 
GAAATTGCAA ACAAGTACGA GTGGCTCGCA CGTGATAACA AGGGAGTCAT CAAGCCCGAC 
CAGTTGATCA AAAGACGTGG AAAGCTGGGT TTGGTCAAGA TTGGAACACC ACAAGAGTTG 
AAGGCATGGT TCGAGAAAAC TGGTGACAGT TATGTGAGAG TTGGTCAAAC CGAGGGACGG 
CTTCACACTT TTATTGTCGA GCCATTTTGT GCACACACTG AAAAGGATGA GATGTACATT 
GCGATCTACA GTGAGAGATT TCGTGACGTC ATCATGTTCT ATGAGCAAGG AGGTGTAGAT 
ATTGGAGATG TTGAGGAAAA GGCTCGCACT GTATCCGTCC CCGTCCAGCT CAACGAGAAT 
GCCATGACCC CCTCGGATGA AGAGCTCACC ACCCTCCTGG GCCCACTCAA GGACAGTGAC 
ATTGTCAGAA GATTTGTGGT AGAGCTCTAC AAAGCCTACA AGGACCTACA TTTCACGTAT 
CTCGAGATTA ATCCATTTGT CCTTTTGAAC AACCAGATTC ATGTTTTGGA TTTGGCAGCT 
AGGCTTGATG AAACCGCCAA TTTCTTGTGC GCAGACAAGT GGAAGAGCCG TCTGACACCA 
TATGGAGGAC CGAACCACGT GGAGTTCCCA GCTCCGTTTG GACGTGATTT GACGTCAGAG 
GAGCAGTACA TTTCGGAGAT GGATGCGAAG ACGGGAGCTT CCTTGAAGCT GACGATTTTG 
AACAGAAAGG GAAGAGTTTG GACGATGGTG GCTGGTGGTG GAGCTTCAGT TGTGTTTACC 
GACACTGTCT GTGACCTCGG TGGAGCATCA GAACTCGCCA ACTACGGAGA ATACTCTGGA 
GACCCGTCTG AATCGCAAAC CTATGAATAC GCAAAGACAC TCCTCTCCGT GATGACCGAA 
GGTACTCCGA GACCTGATGG AAAAGTCCTC ATCATCGGAG GATCCATCGC CAACTTCACA 
AATGTTGCTA AAACTTTTGG TGGAATCGTC AGAGCCTTTG AAACGTTTGT CTCAAAGCTC 
AAAGAGCACA AGGTGACAAT TTTCGTGCGT CGCGGAGGCC CAAACTACCA GGAGGGACTC 
AGAAGAATCA AGGATGCTGC GACAAAGTTG GAGCTGCCAA TTCATGTATT CGGACCTGAG 
ACTCATATGA CGGCGATTGT TGGAGCAGCG CTCGGAGTGA AGCCAATGCC AACAGTTCCG 
ACAGCTCCAC AGACTACCGG ACAGTTTTTG TTGAGCCCTG AGAGAAATAC CGGAGGCACT 
GAGAGAGCTC CACCTTCGCC AGCAGCCAAC GCCACTCCAA CTGAGCACCC ACTCACCACT 
GCCCAACAGA ACAAACTGAA AAGCTTCCGT GGACTCTTCG AGGACGACAC CAAGGCCATC 
ATCTGGGGAC AACAGGCTAA GGCTATTCAA GGAATGCTGG ACTTTGACTA TGTCTGCAGA 
AGAAGCTCCC CGTCGGTTGT CGCGTCCACC TACCCGTTCA CTGGGGACAA CAAGCAGAAG 
TACTACTTTG GGCAAAAGGA AATTCTCATT CCAGCCTATA AGTCGATGGC GAAGGCTTTT 
GCAACTCATC CGGATGCTTC TATCATGGTC ACTTTTGCCT CGATGAGATC TGTATTTGAG 
ACTGTTCTCG AGGCGCTCGA GTTCCCACAA ATCAAGGTTA TTGCGATCAT TGCTGAAGGT 
GTCCCTGAGA ATCAGACTAG AAAGCTGTTG AAGATTGCTC ATGACCGTGG AGTCACCCTT 
GTTGGGCCAG CCACCGTCGG AGGTATCAAG CCGGGATGCT TTAAGATTGG AAACACCGGT 
GGAATGATGG ACAACATCCT TGCCTCAAAG CTCTACCGCC CTGGAAGTGT CGCCTATGTA 
TCTCGCTCCG GAGGAATGTC CAATGAGCTT AACAATATCA TTTCACAAAA CACTAACGGA 
GTCTATGAAG GTATTGCAAT TGGAGGTGAC CGCTATCCGG GAAGCACGTA CACTGATCAC 
GTGATTCGCT ATCAAAATGA TGATCGTGTG AAGATGATTG TTCTTCTCGG TGAGGTCGGT 
GGAGTTGAAG AGTACAAAAT TGTGGATCTC CTCAAGCAGA AGAAGGTGAC AAAGCCATTG 
GTCGCATGGT GCATCGGAAC CTGTGCAGAT CACATCACAT CGGAGGTTCA ATTCGGTCAC 
GCTGGAGCAT CTGCAAATGC TCTCGGAGAA ACCGCTGCCT GCAAAAATGC TGCTCTCCGT 
GCTTCAGGTG CCCTTGTCCC CGAGTCATTT GACGATCTTG GCAACAAAAT CCGTCAGACG 
TACGACGAAC TTGTCAGCCA ACAAATCATC GTTCCACAGC CAGAAGTTCC ACCTCCAGCG 
GTGCCGATGG ATTACGCGTG GGCTCGTGAA CTGGGACTCA TTAGAAAACC AGCTTCCTTC 
ATGACCTCAA TTTGTGATGA GAGAGGAGAA GAGTTGAACT ATGCGGGAGT ACCGATTACA 
AAGGTTTTGG AGTCGGATAT GGGAATCGGT GGAGTGTTGG GATTGTTGTG GTTCCAGAAG 
CGTCTCCCAC CACATGCCAA CAAATTTATC GAGATCTGCT TGATGCTCAC CGCCGATCAC 
GGACCAGCTG TGTCGGGAGC CCATAACACT ATTGTGTGTG CTCGTGCTGG AAAAGATCTG 
ATCTCTTCGC TCACGTCTGG TCTACTCACC ATTGGTGATC GCTTCGGAGG TGCCCTCGAT 
GGAGCCGCTC GTCAGTTCTC TGAGGCTTTT GATCAAGGAT GGTCGGCGAA TCAGTTTGTC 
AGCGAGATGA GAAAGAAGGG AAAGCATATT ATGGGAATTG GACATCGTGT GAAGAGTATC 
AACAACCCTG ACAAACGTGT GGAGATTCTG AAAAGATTTG CCATGGACAA GAAGGAGTTT 
GCCCAGGAGA CGCCGTTGTT CGAGTATGCT CTTGAGGTCG AGAAGATCAC GACCGCCAAA 
AAACCGAACC TCATTCTCAA CGTGGACGGA GCGATTGCGA TCTTATTCGT CGATATCCTT 
CGCCACAGTG GCATGTTCAC CAAGCAGGAG GCCGAGGAGA CAATCGAGAT TGGATCGCTC 
AATGGGCTTT TCGTTTTGGG TAGATCTATT GGATTTATCG GTCACTACTT GGATCAGTCG 
CGTCTGAAGC AGGGATTGTA CCGTCATCCA TGGGATGATA TTTCCTACAT TATGCCGGAG 
AGCAACTTGG TAAAGTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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acly-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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