ACLY cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACLY
Entrez Gene ID	511135
Full Name	ATP citrate lyase
General protein information	Preferred Names ATP citrate lyase Names ATP-citrate synthase ATP-citrate (pro-S-)-lyase citrate cleavage enzyme
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005220713.4	XP_005220770.1	ATP-citrate synthase isoform X1
NM_001037457.1	NP_001032534.1	ATP-citrate synthase
XM_005220715.4	XP_005220772.1	ATP-citrate synthase isoform X2
XM_005220713.3	XP_005220770.1	ATP-citrate synthase isoform X1
XM_005220715.3	XP_005220772.1	ATP-citrate synthase isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Acly	NP_001186225.1
Gallus gallus (chicken)	ACLY	NP_001025711.1
Danio rerio (zebrafish)	aclya	NP_001002649.1
Caenorhabditis elegans (roundworm)	acly-2	NP_506267.1
Magnaporthe oryzae (rice blast fungus)	MGG_06719	XP_003709430.1
Arabidopsis thaliana (thale cress)	ACLB-2	NP_199757.1
Arabidopsis thaliana (thale cress)	ACLB-1	NP_187317.1
Caenorhabditis elegans (roundworm)	D1005.1	NP_508280.1
Rattus norvegicus (Norway rat)	Acly	NP_058683.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010156	XP_319323.3
Oryza sativa (rice)	Os01g0300200	NP_001042817.1
Xenopus tropicalis (tropical clawed frog)	acly	NP_001008028.1
	ACLY	XP_001108114.2
Bos taurus (cattle)	ACLY	NP_001032534.1
Homo sapiens (human)	ACLY	NP_001087.2
Pan troglodytes (chimpanzee)	ACLY	XP_511495.3
Canis lupus familiaris (dog)	ACLY	XP_850198.1
Drosophila melanogaster (fruit fly)	ATPCL	NP_725514.2
Schizosaccharomyces pombe (fission yeast)	SPBC1703.07	NP_596202.1
Neurospora crassa	NCU06785	XP_963626.1

Gene Ontology
Bibliography

Related articles in PubMed

Association of genetic variations in the ACLY gene with growth traits in Chinese beef cattle.
Li MN, Guo X, Bao PJ, Wu XY, Ding XZ, Chu M, Liang CN, Yan P
Genetics and molecular research : GMR15(2)(2016 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we identified three non-synonymous mutations in ACLY exons in five beef cattle populations. Single nucleotide polymorphism (SNP) g.17127C>T is significantly associated with chest girth and body height, and with body slanting length. SNP g.40427T>C is significantly associated with an increase in chest girth. ACLY gene are associated with growth traits in beef cattle in northwest China
Title: Association of genetic variations in the ACLY gene with growth traits in Chinese beef cattle.

The following ACLY gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACLY cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226197	XM_005220713.4 Latest version!	Bos taurus ATP citrate lyase (ACLY), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa226198	XM_005220715.4 Latest version!	Bos taurus ATP citrate lyase (ACLY), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa42218	NM_001037457.1 Latest version!	Bos taurus ATP citrate lyase (ACLY), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30016	XM_005220713.3 Latest version!	Bos taurus ATP citrate lyase (ACLY), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30017	XM_005220715.3 Latest version!	Bos taurus ATP citrate lyase (ACLY), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACLY ( XM_005220713.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220770.1 Bos taurus ATP citrate lyase (ACLY), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226197
Clone ID Related Accession (Same CDS sequence)	XM_005220713.4
Accession Version	XM_005220713.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-citrate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220713.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCA AGGCGATTTC TGAGCAGACA GGCAAAGAGC TCCTCTACAA GTACATCTGC 
ACCACCTCGG CCATCCAGAA CCGCTTCAAG TATGCCCGGG TCACCCCTGA CACAGACTGG 
GCCCGCCTGT TGCAGGACCA CCCGTGGCTG CTCAGCCAGA GCTTGGTGGT CAAGCCAGAC 
CAGCTGATCA AACGGCGTGG AAAGCTGGGC CTAATTGGGG TCAACCTCAC TCTGGATGGA 
GTCAAGTCCT GGCTGAAGCC ACGCCTGGGA CAGGAAGCTA CAGTTGGCAA GGCCACAGGC 
TTCCTCAAGA ACTTCCTGAT CGAACCCTTC GTCCCCCACA CTCAGGAGGA AGAGTTCTAC 
GTCTGCATCT ATGCTACCCG AGAAGGGGAC TATGTCCTGT TCCACCACGA GGGTGGGGTG 
GACGTGGGCG ACGTGGATGC TAAAGCCCAG AAACTACTTG TCGGCGTCGA TGAGAAGCTG 
AATCCTGAGG ACATCAAGAA ACACCTGCTG GTCCACGCCC CCGAAGACAA GAAAGAAATT 
CTGGCCAGCT TTATCTCCGG CCTCTTCAAT TTCTATGAGG ATCTGTACTT CACCTACCTC 
GAGATCAACC CCCTTGTCGT GACCAAAGAT GGCGTCTACG TCCTTGACTT GGCCGCCAAG 
GTGGATGCCA CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATAGA GTTCCCACCC 
CCCTTCGGAC GGGAGGCTTA CCCAGAGGAA GCCTACATCG CAGATCTGGA CGCCAAAAGT 
GGGGCAAGCT TGAAGCTGAC TTTACTGAAT CCCAAGGGGA GAATCTGGAC CATGGTGGCC 
GGAGGGGGTG CCTCTGTCGT GTACAGTGAC ACCATCTGCG ACCTGGGGGG TGTCAACGAG 
CTGGCAAACT ACGGGGAGTA CTCGGGCGCC CCCAGCGAGC AGCAGACCTA TGACTACGCC 
AAGACGATCC TCTCCCTTAT GACCCGCGAG AAGCACCCAG ATGGCAAGAT CCTCATCATC 
GGAGGCAGCA TCGCAAACTT CACCAACGTG GCTGCCACAT TCAAGGGCAT CGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CGATCTTTGT CCGAAGAGGT 
GGCCCCAACT ATCAGGAGGG CTTACGGGTC ATGGGAGAAG TCGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTCGGCAC TGAGACTCAC ATGACGGCCA TCGTGGGCAT GGCCCTGGGC 
CATCGGCCCA TCCCCAATCA GCCGCCCACA GCGGCCCACA CCGCAAACTT CCTCCTCAAC 
GCCAGCGGGA GTACTTCAAC TCCAGCCCCC AGCAGGACGG CATCTTTTTC TGAGTCCAGA 
ACTGATGAGG TGGCCCCTGC CAAGAAGGCC AAGCCTGCCA TGCTCCAAGC CTCCAGCCAT 
CAACGGGTCA CTCAGTCGCC TTGGTCATTT TCTTTGACAG ATTCAGTCCC AAGTCCAAGA 
CCCCTGCAAG GAAAGAGCGC CACCCTCTTC AGCCGCCACA CCAAGGCCAT CGTGTGGGGC 
ATGCAGACCC GGGCTGTGCA GGGCATGCTG GACTTCGACT ACGTCTGCTC CCGCGACGAG 
CCCTCAGTGG CTGCCATGGT CTACCCGTTC ACTGGGGACC ACAAACAGAA GTTTTACTGG 
GGTCACAAGG AGATCCTGAT CCCCGTCTTT AAGAACATGG CTGATGCCAT GAAGAAGCAC 
CCAGAGGTAG ACGTGCTAAT CAACTTTGCC TCCCTGCGCT CAGCCTACGA CAGCACCATG 
GAGACCATGA ATTACGCTCA GATCCGGACC ATCGCCATCA TAGCCGAAGG CATCCCTGAG 
GCGCTCACGA GGAAGCTGAT CAAGAAGGCA GACCAGAAGG GAGTGACCAT CATTGGACCT 
GCCACCGTTG GTGGCATCAA GCCCGGGTGC TTTAAGATCG GGAACACCGG CGGGATGCTG 
GACAACATCC TGGCCTCTAA GCTGTACCGC CCAGGCAGTG TGGCCTATGT CTCGCGTTCG 
GGTGGCATGT CCAACGAGCT CAACAATATC ATCTCTCGAA CCACAGATGG CGTCTACGAG 
GGCGTGGCCA TTGGTGGGGA CAGGTACCCC GGCTCAACGT TTATGGATCA CGTGTTACGT 
TATCAGGACA CTGCAGGAGT CAAAATGATT GTGGTTCTTG GAGAGATAGG GGGCACAGAG 
GAGTATAAGA TCTGCCGGGG CGTCACGGAG GGCCGCATCA CCAAGCCCGT GGTCTGCTGG 
TGCATCGGGA CCTGTGCGGC CATGTTCTCC TCCGAGGTCC AGTTCGGCCA CGCTGGAGCT 
TGTGCCAACC AGGCTTCTGA AACCGCAGTT GCCAAGAACC AGGCCTTGAA GGAGGCTGGA 
GTGTTTGTGC CCCGGAGCTT CGATGAGCTT GGAGAAATCA TCCAGTCCGT GTACGAGGAT 
CTCGTGGCCA GAGGAGTCAT TGTGCCTGCT CAGGAGGTTC CGCCCCCCAC AGTACCCATG 
GACTACTCTT GGGCCCGGGA ATTGGGCTTG ATCCGCAAAC CTGCCTCGTT CATGACCAGC 
ATCTGTGACG AGCGAGGACA GGAGCTCATC TACGCGGGCA TGCCCATCAC TGAGGTCTTC 
AAGGAGGAGA TGGGCATTGG CGGGGTCCTC GGCCTCCTCT GGTTCCAGAA AAGGTTGCCC 
AAATACTCCT GCCAGTTCAT TGAGATGTGC CTGATGGTGA CGGCTGATCA CGGGCCTGCC 
GTCTCTGGGG CTCATAACAC CATCATCTGC GCTCGAGCCG GCAAGGACCT CGTCTCCAGC 
CTCACCTCGG GACTGCTTAC CATTGGCGAC CGGTTTGGGG GTGCCCTGGA TGCAGCAGCC 
AAGATGTTCA GCAAGGCCTT TGACAGTGGC ATCATCCCCA TGGAGTTTGT GAACAAGATG 
AAGAAGGAAG GAAAGCTTAT CATGGGCATT GGTCACCGAG TGAAGTCGAT CAACAACCCA 
GACATGCGCG TGCAGATTCT CAAAGATTAT GTCAGACAAC ACTTCCCTGC CACCCCACTG 
CTCGACTACG CACTGGAAGT AGAGAAGATT ACCACTTCCA AGAAACCAAA CCTTATCCTG 
AATGTGGACG GTTTAATCGG AGTTGCATTT GTAGACATGC TTAGACACTG CGGGTCCTTC 
ACTCGGGAGG AAGCTGATGA ATATATTGAC ATTGGAGCCC TCAACGGCAT CTTTGTGCTG 
GGAAGGAGCA TGGGCTTCAT TGGACACTAT CTTGATCAGA AGAGGCTGAA GCAGGGGCTC 
TACCGTCACC CATGGGATGA TATTTCATAT GTTCTTCCGG AACACATGAG CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220770.1
CDS	205..3561
Translation	MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQSLVVKPDQLIKRRGKLGLIGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPFVPHTQEEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYVLDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRTDEVAPAKKAKPAMLQASSHQRVTQSPWSFSLTDSVPSPRPLQGKSATLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTAGVKMIVVLGEIGGTEEYKICRGVTEGRITKPVVCWCIGTCAAMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVARGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRHCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

Target ORF information:

RefSeq Version	XM_005220713.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP citrate lyase (ACLY), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220713.4

ORF Insert Sequence:

ATGTCGGCCA AGGCGATTTC TGAGCAGACA GGCAAAGAGC TCCTCTACAA GTACATCTGC 
ACCACCTCGG CCATCCAGAA CCGCTTCAAG TATGCCCGGG TCACCCCTGA CACAGACTGG 
GCCCGCCTGT TGCAGGACCA CCCGTGGCTG CTCAGCCAGA GCTTGGTGGT CAAGCCAGAC 
CAGCTGATCA AACGGCGTGG AAAGCTGGGC CTAATTGGGG TCAACCTCAC TCTGGATGGA 
GTCAAGTCCT GGCTGAAGCC ACGCCTGGGA CAGGAAGCTA CAGTTGGCAA GGCCACAGGC 
TTCCTCAAGA ACTTCCTGAT CGAACCCTTC GTCCCCCACA CTCAGGAGGA AGAGTTCTAC 
GTCTGCATCT ATGCTACCCG AGAAGGGGAC TATGTCCTGT TCCACCACGA GGGTGGGGTG 
GACGTGGGCG ACGTGGATGC TAAAGCCCAG AAACTACTTG TCGGCGTCGA TGAGAAGCTG 
AATCCTGAGG ACATCAAGAA ACACCTGCTG GTCCACGCCC CCGAAGACAA GAAAGAAATT 
CTGGCCAGCT TTATCTCCGG CCTCTTCAAT TTCTATGAGG ATCTGTACTT CACCTACCTC 
GAGATCAACC CCCTTGTCGT GACCAAAGAT GGCGTCTACG TCCTTGACTT GGCCGCCAAG 
GTGGATGCCA CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATAGA GTTCCCACCC 
CCCTTCGGAC GGGAGGCTTA CCCAGAGGAA GCCTACATCG CAGATCTGGA CGCCAAAAGT 
GGGGCAAGCT TGAAGCTGAC TTTACTGAAT CCCAAGGGGA GAATCTGGAC CATGGTGGCC 
GGAGGGGGTG CCTCTGTCGT GTACAGTGAC ACCATCTGCG ACCTGGGGGG TGTCAACGAG 
CTGGCAAACT ACGGGGAGTA CTCGGGCGCC CCCAGCGAGC AGCAGACCTA TGACTACGCC 
AAGACGATCC TCTCCCTTAT GACCCGCGAG AAGCACCCAG ATGGCAAGAT CCTCATCATC 
GGAGGCAGCA TCGCAAACTT CACCAACGTG GCTGCCACAT TCAAGGGCAT CGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CGATCTTTGT CCGAAGAGGT 
GGCCCCAACT ATCAGGAGGG CTTACGGGTC ATGGGAGAAG TCGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTCGGCAC TGAGACTCAC ATGACGGCCA TCGTGGGCAT GGCCCTGGGC 
CATCGGCCCA TCCCCAATCA GCCGCCCACA GCGGCCCACA CCGCAAACTT CCTCCTCAAC 
GCCAGCGGGA GTACTTCAAC TCCAGCCCCC AGCAGGACGG CATCTTTTTC TGAGTCCAGA 
ACTGATGAGG TGGCCCCTGC CAAGAAGGCC AAGCCTGCCA TGCTCCAAGC CTCCAGCCAT 
CAACGGGTCA CTCAGTCGCC TTGGTCATTT TCTTTGACAG ATTCAGTCCC AAGTCCAAGA 
CCCCTGCAAG GAAAGAGCGC CACCCTCTTC AGCCGCCACA CCAAGGCCAT CGTGTGGGGC 
ATGCAGACCC GGGCTGTGCA GGGCATGCTG GACTTCGACT ACGTCTGCTC CCGCGACGAG 
CCCTCAGTGG CTGCCATGGT CTACCCGTTC ACTGGGGACC ACAAACAGAA GTTTTACTGG 
GGTCACAAGG AGATCCTGAT CCCCGTCTTT AAGAACATGG CTGATGCCAT GAAGAAGCAC 
CCAGAGGTAG ACGTGCTAAT CAACTTTGCC TCCCTGCGCT CAGCCTACGA CAGCACCATG 
GAGACCATGA ATTACGCTCA GATCCGGACC ATCGCCATCA TAGCCGAAGG CATCCCTGAG 
GCGCTCACGA GGAAGCTGAT CAAGAAGGCA GACCAGAAGG GAGTGACCAT CATTGGACCT 
GCCACCGTTG GTGGCATCAA GCCCGGGTGC TTTAAGATCG GGAACACCGG CGGGATGCTG 
GACAACATCC TGGCCTCTAA GCTGTACCGC CCAGGCAGTG TGGCCTATGT CTCGCGTTCG 
GGTGGCATGT CCAACGAGCT CAACAATATC ATCTCTCGAA CCACAGATGG CGTCTACGAG 
GGCGTGGCCA TTGGTGGGGA CAGGTACCCC GGCTCAACGT TTATGGATCA CGTGTTACGT 
TATCAGGACA CTGCAGGAGT CAAAATGATT GTGGTTCTTG GAGAGATAGG GGGCACAGAG 
GAGTATAAGA TCTGCCGGGG CGTCACGGAG GGCCGCATCA CCAAGCCCGT GGTCTGCTGG 
TGCATCGGGA CCTGTGCGGC CATGTTCTCC TCCGAGGTCC AGTTCGGCCA CGCTGGAGCT 
TGTGCCAACC AGGCTTCTGA AACCGCAGTT GCCAAGAACC AGGCCTTGAA GGAGGCTGGA 
GTGTTTGTGC CCCGGAGCTT CGATGAGCTT GGAGAAATCA TCCAGTCCGT GTACGAGGAT 
CTCGTGGCCA GAGGAGTCAT TGTGCCTGCT CAGGAGGTTC CGCCCCCCAC AGTACCCATG 
GACTACTCTT GGGCCCGGGA ATTGGGCTTG ATCCGCAAAC CTGCCTCGTT CATGACCAGC 
ATCTGTGACG AGCGAGGACA GGAGCTCATC TACGCGGGCA TGCCCATCAC TGAGGTCTTC 
AAGGAGGAGA TGGGCATTGG CGGGGTCCTC GGCCTCCTCT GGTTCCAGAA AAGGTTGCCC 
AAATACTCCT GCCAGTTCAT TGAGATGTGC CTGATGGTGA CGGCTGATCA CGGGCCTGCC 
GTCTCTGGGG CTCATAACAC CATCATCTGC GCTCGAGCCG GCAAGGACCT CGTCTCCAGC 
CTCACCTCGG GACTGCTTAC CATTGGCGAC CGGTTTGGGG GTGCCCTGGA TGCAGCAGCC 
AAGATGTTCA GCAAGGCCTT TGACAGTGGC ATCATCCCCA TGGAGTTTGT GAACAAGATG 
AAGAAGGAAG GAAAGCTTAT CATGGGCATT GGTCACCGAG TGAAGTCGAT CAACAACCCA 
GACATGCGCG TGCAGATTCT CAAAGATTAT GTCAGACAAC ACTTCCCTGC CACCCCACTG 
CTCGACTACG CACTGGAAGT AGAGAAGATT ACCACTTCCA AGAAACCAAA CCTTATCCTG 
AATGTGGACG GTTTAATCGG AGTTGCATTT GTAGACATGC TTAGACACTG CGGGTCCTTC 
ACTCGGGAGG AAGCTGATGA ATATATTGAC ATTGGAGCCC TCAACGGCAT CTTTGTGCTG 
GGAAGGAGCA TGGGCTTCAT TGGACACTAT CTTGATCAGA AGAGGCTGAA GCAGGGGCTC 
TACCGTCACC CATGGGATGA TATTTCATAT GTTCTTCCGG AACACATGAG CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACLY ( XM_005220715.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220772.1 Bos taurus ATP citrate lyase (ACLY), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa226198
Clone ID Related Accession (Same CDS sequence)	XM_005220715.4
Accession Version	XM_005220715.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP-citrate synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220715.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCA AGGCGATTTC TGAGCAGACA GGCAAAGAGC TCCTCTACAA GTACATCTGC 
ACCACCTCGG CCATCCAGAA CCGCTTCAAG TATGCCCGGG TCACCCCTGA CACAGACTGG 
GCCCGCCTGT TGCAGGACCA CCCGTGGCTG CTCAGCCAGA GCTTGGTGGT CAAGCCAGAC 
CAGCTGATCA AACGGCGTGG AAAGCTGGGC CTAATTGGGG TCAACCTCAC TCTGGATGGA 
GTCAAGTCCT GGCTGAAGCC ACGCCTGGGA CAGGAAGCTA CAGTTGGCAA GGCCACAGGC 
TTCCTCAAGA ACTTCCTGAT CGAACCCTTC GTCCCCCACA CTCAGGAGGA AGAGTTCTAC 
GTCTGCATCT ATGCTACCCG AGAAGGGGAC TATGTCCTGT TCCACCACGA GGGTGGGGTG 
GACGTGGGCG ACGTGGATGC TAAAGCCCAG AAACTACTTG TCGGCGTCGA TGAGAAGCTG 
AATCCTGAGG ACATCAAGAA ACACCTGCTG GTCCACGCCC CCGAAGACAA GAAAGAAATT 
CTGGCCAGCT TTATCTCCGG CCTCTTCAAT TTCTATGAGG ATCTGTACTT CACCTACCTC 
GAGATCAACC CCCTTGTCGT GACCAAAGAT GGCGTCTACG TCCTTGACTT GGCCGCCAAG 
GTGGATGCCA CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATAGA GTTCCCACCC 
CCCTTCGGAC GGGAGGCTTA CCCAGAGGAA GCCTACATCG CAGATCTGGA CGCCAAAAGT 
GGGGCAAGCT TGAAGCTGAC TTTACTGAAT CCCAAGGGGA GAATCTGGAC CATGGTGGCC 
GGAGGGGGTG CCTCTGTCGT GTACAGTGAC ACCATCTGCG ACCTGGGGGG TGTCAACGAG 
CTGGCAAACT ACGGGGAGTA CTCGGGCGCC CCCAGCGAGC AGCAGACCTA TGACTACGCC 
AAGACGATCC TCTCCCTTAT GACCCGCGAG AAGCACCCAG ATGGCAAGAT CCTCATCATC 
GGAGGCAGCA TCGCAAACTT CACCAACGTG GCTGCCACAT TCAAGGGCAT CGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CGATCTTTGT CCGAAGAGGT 
GGCCCCAACT ATCAGGAGGG CTTACGGGTC ATGGGAGAAG TCGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTCGGCAC TGAGACTCAC ATGACGGCCA TCGTGGGCAT GGCCCTGGGC 
CATCGGCCCA TCCCCAATCA GCCGCCCACA GCGGCCCACA CCGCAAACTT CCTCCTCAAC 
GCCAGCGGGA GTACTTCAAC TCCAGCCCCC AGCAGGACGG CATCTTTTTC TGAGTCCAGA 
ACTGATGAGG TGGCCCCTGC CAAGAAGGCC AAGCCTGCCA TGCTCCAAGA TTCAGTCCCA 
AGTCCAAGAC CCCTGCAAGG AAAGAGCGCC ACCCTCTTCA GCCGCCACAC CAAGGCCATC 
GTGTGGGGCA TGCAGACCCG GGCTGTGCAG GGCATGCTGG ACTTCGACTA CGTCTGCTCC 
CGCGACGAGC CCTCAGTGGC TGCCATGGTC TACCCGTTCA CTGGGGACCA CAAACAGAAG 
TTTTACTGGG GTCACAAGGA GATCCTGATC CCCGTCTTTA AGAACATGGC TGATGCCATG 
AAGAAGCACC CAGAGGTAGA CGTGCTAATC AACTTTGCCT CCCTGCGCTC AGCCTACGAC 
AGCACCATGG AGACCATGAA TTACGCTCAG ATCCGGACCA TCGCCATCAT AGCCGAAGGC 
ATCCCTGAGG CGCTCACGAG GAAGCTGATC AAGAAGGCAG ACCAGAAGGG AGTGACCATC 
ATTGGACCTG CCACCGTTGG TGGCATCAAG CCCGGGTGCT TTAAGATCGG GAACACCGGC 
GGGATGCTGG ACAACATCCT GGCCTCTAAG CTGTACCGCC CAGGCAGTGT GGCCTATGTC 
TCGCGTTCGG GTGGCATGTC CAACGAGCTC AACAATATCA TCTCTCGAAC CACAGATGGC 
GTCTACGAGG GCGTGGCCAT TGGTGGGGAC AGGTACCCCG GCTCAACGTT TATGGATCAC 
GTGTTACGTT ATCAGGACAC TGCAGGAGTC AAAATGATTG TGGTTCTTGG AGAGATAGGG 
GGCACAGAGG AGTATAAGAT CTGCCGGGGC GTCACGGAGG GCCGCATCAC CAAGCCCGTG 
GTCTGCTGGT GCATCGGGAC CTGTGCGGCC ATGTTCTCCT CCGAGGTCCA GTTCGGCCAC 
GCTGGAGCTT GTGCCAACCA GGCTTCTGAA ACCGCAGTTG CCAAGAACCA GGCCTTGAAG 
GAGGCTGGAG TGTTTGTGCC CCGGAGCTTC GATGAGCTTG GAGAAATCAT CCAGTCCGTG 
TACGAGGATC TCGTGGCCAG AGGAGTCATT GTGCCTGCTC AGGAGGTTCC GCCCCCCACA 
GTACCCATGG ACTACTCTTG GGCCCGGGAA TTGGGCTTGA TCCGCAAACC TGCCTCGTTC 
ATGACCAGCA TCTGTGACGA GCGAGGACAG GAGCTCATCT ACGCGGGCAT GCCCATCACT 
GAGGTCTTCA AGGAGGAGAT GGGCATTGGC GGGGTCCTCG GCCTCCTCTG GTTCCAGAAA 
AGGTTGCCCA AATACTCCTG CCAGTTCATT GAGATGTGCC TGATGGTGAC GGCTGATCAC 
GGGCCTGCCG TCTCTGGGGC TCATAACACC ATCATCTGCG CTCGAGCCGG CAAGGACCTC 
GTCTCCAGCC TCACCTCGGG ACTGCTTACC ATTGGCGACC GGTTTGGGGG TGCCCTGGAT 
GCAGCAGCCA AGATGTTCAG CAAGGCCTTT GACAGTGGCA TCATCCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG AAAGCTTATC ATGGGCATTG GTCACCGAGT GAAGTCGATC 
AACAACCCAG ACATGCGCGT GCAGATTCTC AAAGATTATG TCAGACAACA CTTCCCTGCC 
ACCCCACTGC TCGACTACGC ACTGGAAGTA GAGAAGATTA CCACTTCCAA GAAACCAAAC 
CTTATCCTGA ATGTGGACGG TTTAATCGGA GTTGCATTTG TAGACATGCT TAGACACTGC 
GGGTCCTTCA CTCGGGAGGA AGCTGATGAA TATATTGACA TTGGAGCCCT CAACGGCATC 
TTTGTGCTGG GAAGGAGCAT GGGCTTCATT GGACACTATC TTGATCAGAA GAGGCTGAAG 
CAGGGGCTCT ACCGTCACCC ATGGGATGAT ATTTCATATG TTCTTCCGGA ACACATGAGC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220772.1
CDS	200..3505
Translation	MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQSLVVKPDQLIKRRGKLGLIGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPFVPHTQEEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYVLDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRTDEVAPAKKAKPAMLQDSVPSPRPLQGKSATLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTAGVKMIVVLGEIGGTEEYKICRGVTEGRITKPVVCWCIGTCAAMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVARGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRHCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

Target ORF information:

RefSeq Version	XM_005220715.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP citrate lyase (ACLY), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220715.4

ORF Insert Sequence:

ATGTCGGCCA AGGCGATTTC TGAGCAGACA GGCAAAGAGC TCCTCTACAA GTACATCTGC 
ACCACCTCGG CCATCCAGAA CCGCTTCAAG TATGCCCGGG TCACCCCTGA CACAGACTGG 
GCCCGCCTGT TGCAGGACCA CCCGTGGCTG CTCAGCCAGA GCTTGGTGGT CAAGCCAGAC 
CAGCTGATCA AACGGCGTGG AAAGCTGGGC CTAATTGGGG TCAACCTCAC TCTGGATGGA 
GTCAAGTCCT GGCTGAAGCC ACGCCTGGGA CAGGAAGCTA CAGTTGGCAA GGCCACAGGC 
TTCCTCAAGA ACTTCCTGAT CGAACCCTTC GTCCCCCACA CTCAGGAGGA AGAGTTCTAC 
GTCTGCATCT ATGCTACCCG AGAAGGGGAC TATGTCCTGT TCCACCACGA GGGTGGGGTG 
GACGTGGGCG ACGTGGATGC TAAAGCCCAG AAACTACTTG TCGGCGTCGA TGAGAAGCTG 
AATCCTGAGG ACATCAAGAA ACACCTGCTG GTCCACGCCC CCGAAGACAA GAAAGAAATT 
CTGGCCAGCT TTATCTCCGG CCTCTTCAAT TTCTATGAGG ATCTGTACTT CACCTACCTC 
GAGATCAACC CCCTTGTCGT GACCAAAGAT GGCGTCTACG TCCTTGACTT GGCCGCCAAG 
GTGGATGCCA CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATAGA GTTCCCACCC 
CCCTTCGGAC GGGAGGCTTA CCCAGAGGAA GCCTACATCG CAGATCTGGA CGCCAAAAGT 
GGGGCAAGCT TGAAGCTGAC TTTACTGAAT CCCAAGGGGA GAATCTGGAC CATGGTGGCC 
GGAGGGGGTG CCTCTGTCGT GTACAGTGAC ACCATCTGCG ACCTGGGGGG TGTCAACGAG 
CTGGCAAACT ACGGGGAGTA CTCGGGCGCC CCCAGCGAGC AGCAGACCTA TGACTACGCC 
AAGACGATCC TCTCCCTTAT GACCCGCGAG AAGCACCCAG ATGGCAAGAT CCTCATCATC 
GGAGGCAGCA TCGCAAACTT CACCAACGTG GCTGCCACAT TCAAGGGCAT CGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CGATCTTTGT CCGAAGAGGT 
GGCCCCAACT ATCAGGAGGG CTTACGGGTC ATGGGAGAAG TCGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTCGGCAC TGAGACTCAC ATGACGGCCA TCGTGGGCAT GGCCCTGGGC 
CATCGGCCCA TCCCCAATCA GCCGCCCACA GCGGCCCACA CCGCAAACTT CCTCCTCAAC 
GCCAGCGGGA GTACTTCAAC TCCAGCCCCC AGCAGGACGG CATCTTTTTC TGAGTCCAGA 
ACTGATGAGG TGGCCCCTGC CAAGAAGGCC AAGCCTGCCA TGCTCCAAGA TTCAGTCCCA 
AGTCCAAGAC CCCTGCAAGG AAAGAGCGCC ACCCTCTTCA GCCGCCACAC CAAGGCCATC 
GTGTGGGGCA TGCAGACCCG GGCTGTGCAG GGCATGCTGG ACTTCGACTA CGTCTGCTCC 
CGCGACGAGC CCTCAGTGGC TGCCATGGTC TACCCGTTCA CTGGGGACCA CAAACAGAAG 
TTTTACTGGG GTCACAAGGA GATCCTGATC CCCGTCTTTA AGAACATGGC TGATGCCATG 
AAGAAGCACC CAGAGGTAGA CGTGCTAATC AACTTTGCCT CCCTGCGCTC AGCCTACGAC 
AGCACCATGG AGACCATGAA TTACGCTCAG ATCCGGACCA TCGCCATCAT AGCCGAAGGC 
ATCCCTGAGG CGCTCACGAG GAAGCTGATC AAGAAGGCAG ACCAGAAGGG AGTGACCATC 
ATTGGACCTG CCACCGTTGG TGGCATCAAG CCCGGGTGCT TTAAGATCGG GAACACCGGC 
GGGATGCTGG ACAACATCCT GGCCTCTAAG CTGTACCGCC CAGGCAGTGT GGCCTATGTC 
TCGCGTTCGG GTGGCATGTC CAACGAGCTC AACAATATCA TCTCTCGAAC CACAGATGGC 
GTCTACGAGG GCGTGGCCAT TGGTGGGGAC AGGTACCCCG GCTCAACGTT TATGGATCAC 
GTGTTACGTT ATCAGGACAC TGCAGGAGTC AAAATGATTG TGGTTCTTGG AGAGATAGGG 
GGCACAGAGG AGTATAAGAT CTGCCGGGGC GTCACGGAGG GCCGCATCAC CAAGCCCGTG 
GTCTGCTGGT GCATCGGGAC CTGTGCGGCC ATGTTCTCCT CCGAGGTCCA GTTCGGCCAC 
GCTGGAGCTT GTGCCAACCA GGCTTCTGAA ACCGCAGTTG CCAAGAACCA GGCCTTGAAG 
GAGGCTGGAG TGTTTGTGCC CCGGAGCTTC GATGAGCTTG GAGAAATCAT CCAGTCCGTG 
TACGAGGATC TCGTGGCCAG AGGAGTCATT GTGCCTGCTC AGGAGGTTCC GCCCCCCACA 
GTACCCATGG ACTACTCTTG GGCCCGGGAA TTGGGCTTGA TCCGCAAACC TGCCTCGTTC 
ATGACCAGCA TCTGTGACGA GCGAGGACAG GAGCTCATCT ACGCGGGCAT GCCCATCACT 
GAGGTCTTCA AGGAGGAGAT GGGCATTGGC GGGGTCCTCG GCCTCCTCTG GTTCCAGAAA 
AGGTTGCCCA AATACTCCTG CCAGTTCATT GAGATGTGCC TGATGGTGAC GGCTGATCAC 
GGGCCTGCCG TCTCTGGGGC TCATAACACC ATCATCTGCG CTCGAGCCGG CAAGGACCTC 
GTCTCCAGCC TCACCTCGGG ACTGCTTACC ATTGGCGACC GGTTTGGGGG TGCCCTGGAT 
GCAGCAGCCA AGATGTTCAG CAAGGCCTTT GACAGTGGCA TCATCCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG AAAGCTTATC ATGGGCATTG GTCACCGAGT GAAGTCGATC 
AACAACCCAG ACATGCGCGT GCAGATTCTC AAAGATTATG TCAGACAACA CTTCCCTGCC 
ACCCCACTGC TCGACTACGC ACTGGAAGTA GAGAAGATTA CCACTTCCAA GAAACCAAAC 
CTTATCCTGA ATGTGGACGG TTTAATCGGA GTTGCATTTG TAGACATGCT TAGACACTGC 
GGGTCCTTCA CTCGGGAGGA AGCTGATGAA TATATTGACA TTGGAGCCCT CAACGGCATC 
TTTGTGCTGG GAAGGAGCAT GGGCTTCATT GGACACTATC TTGATCAGAA GAGGCTGAAG 
CAGGGGCTCT ACCGTCACCC ATGGGATGAT ATTTCATATG TTCTTCCGGA ACACATGAGC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACLY ( NM_001037457.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001032534.1 Bos taurus ATP citrate lyase (ACLY), mRNA.

Price & Availability↑

CloneID	OBa42218
Clone ID Related Accession (Same CDS sequence)	NM_001037457.1
Accession Version	NM_001037457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	ATP-citrate synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC108138.1. On or before Nov 27, 2005 this sequence version replaced XM_873089.1, XM_873189.1, XM_588412.2, XM_873390.1, XM_873490.1, XM_864567.1, XM_873684.1, XM_873779.1, XM_873874.1, XM_873970.1, XM_874059.1, XM_874151.1, XM_874241.1, XM_874326.1, XM_874402.1. ##Evidence-Data-START## Transcript exon combination :: BC108138.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTCGGCCA AGGCGATTTC TGAGCAGACA GGCAAAGAGC TCCTCTACAA GTACATCTGC 
ACCACCTCGG CCATCCAGAA CCGCTTCAAG TATGCCCGGG TCACCCCTGA CACAGACTGG 
GCCCGCCTGT TGCAGGACCA CCCGTGGCTG CTCAGCCAGA GCTTGGTGGT CAAGCCAGAC 
CAGCTGATCA AACGGCGTGG AAAGCTGGGC CTAATTGGGG TCAACCTCAC TCTGGATGGA 
GTCAAGTCCT GGCTGAAGCC ACGCCTGGGA CAGGAAGCTA CAGTTGGCAA GGCCACAGGC 
TTCCTCAAGA ACTTCCTGAT CGAACCCTTC GTCCCCCACA CTCAGGAGGA AGAGTTCTAC 
GTCTGCATCT ATGCTACCCG AGAAGGGGAC TATGTCCTGT TCCACCACGA GGGTGGGGTG 
GACGTGGGCG ACGTGGATGC TAAAGCCCAG AAACTACTTG TCGGCGTCGA TGAGAAGCTG 
AATCCTGAGG ACATCAAGAA ACACCTGCTG GTCCACGCCC CCGAAGACAA GAAAGAAATT 
CTGGCCAGCT TTATCTCCGG CCTCTTCAAT TTCTATGAGG ATCTGTACTT CACCTACCTC 
GAGATCAACC CCCTTGTCGT GACCAAAGAT GGCGTCTACG TCCTTGACTT GGCCGCCAAG 
GTGGATGCCA CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATAGA GTTCCCACCC 
CCCTTCGGAC GGGAGGCTTA CCCAGAGGAA GCCTACATCG CAGATCTGGA CGCCAAAAGT 
GGGGCAAGCT TGAAGCTGAC TTTACTGAAT CCCAAGGGGA GAATCTGGAC CATGGTGGCC 
GGAGGGGGTG CCTCTGTCGT GTACAGTGAC ACCATCTGCG ACCTGGGGGG TGTCAACGAG 
CTGGCAAACT ACGGGGAGTA CTCGGGCGCC CCCAGCGAGC AGCAGACCTA TGACTACGCC 
AAGACGATCC TCTCCCTTAT GACCCGCGAG AAGCACCCAG ATGGCAAGAT CCTCATCATC 
GGAGGCAGCA TCGCAAACTT CACCAACGTG GCTGCCACAT TCAAGGGCAT CGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CGATCTTTGT CCGAAGAGGT 
GGCCCCAACT ATCAGGAGGG CTTACGGGTC ATGGGAGAAG TCGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTCGGCAC TGAGACTCAC ATGACGGCCA TCGTGGGCAT GGCCCTGGGC 
CATCGGCCCA TCCCCAATCA GCCGCCCACA GCGGCCCACA CCGCAAACTT CCTCCTCAAC 
GCCAGCGGGA GTACTTCAAC TCCAGCCCCC AGCAGGACGG CATCTTTTTC TGAGTCCAGA 
ACTGATGAGG TGGCCCCTGC CAAGAAGGCC AAGCCTGCCA TGCTCCAAGG AAAGAGCGCC 
ACCCTCTTCA GCCGCCACAC CAAGGCCATC GTGTGGGGCA TGCAGACCCG GGCTGTGCAG 
GGCATGCTGG ACTTCGACTA CGTCTGCTCC CGCGACGAGC CCTCAGTGGC TGCCATGGTC 
TACCCGTTCA CTGGGGACCA CAAACAGAAG TTTTACTGGG GTCACAAGGA GATCCTGATC 
CCCGTCTTTA AGAACATGGC TGATGCCATG AAGAAGCACC CAGAGGTAGA CGTGCTAATC 
AACTTTGCCT CCCTGCGCTC AGCCTACGAC AGCACCATGG AGACCATGAA TTACGCTCAG 
ATCCGGACCA TCGCCATCAT AGCCGAAGGC ATCCCTGAGG CGCTCACGAG GAAGCTGATC 
AAGAAGGCAG ACCAGAAGGG AGTGACCATC ATTGGACCTG CCACCGTTGG TGGCATCAAG 
CCCGGGTGCT TTAAGATCGG GAACACCGGC GGGATGCTGG ACAACATCCT GGCCTCTAAG 
CTGTACCGCC CAGGCAGTGT GGCCTATGTC TCGCGTTCGG GTGGCATGTC CAACGAGCTC 
AACAATATCA TCTCTCGAAC CACAGATGGC GTCTACGAGG GCGTGGCCAT TGGTGGGGAC 
AGGTACCCCG GCTCAACGTT TATGGATCAC GTGTTACGTT ATCAGGACAC TGCAGGAGTC 
AAAATGATTG TGGTTCTTGG AGAGATAGGG GGCACAGAGG AGTATAAGAT CTGCCGGGGC 
GTCACGGAGG GCCGCATCAC CAAGCCCGTG GTCTGCTGGT GCATCGGGAC CTGTGCGGCC 
ATGTTCTCCT CCGAGGTCCA GTTCGGCCAC GCTGGAGCTT GTGCCAACCA GGCTTCTGAA 
ACCGCAGTTG CCAAGAACCA GGCCTTGAAG GAGGCTGGAG TGTTTGTGCC CCGGAGCTTC 
GATGAGCTTG GAGAAATCAT CCAGTCCGTG TACGAGGATC TCGTGGCCAG AGGAGTCATT 
GTGCCTGCTC AGGAGGTTCC GCCCCCCACA GTACCCATGG ACTACTCTTG GGCCCGGGAA 
TTGGGCTTGA TCCGCAAACC TGCCTCGTTC ATGACCAGCA TCTGTGACGA GCGAGGACAG 
GAGCTCATCT ACGCGGGCAT GCCCATCACT GAGGTCTTCA AGGAGGAGAT GGGCATTGGC 
GGGGTCCTCG GCCTCCTCTG GTTCCAGAAA AGGTTGCCCA AATACTCCTG CCAGTTCATT 
GAGATGTGCC TGATGGTGAC GGCTGATCAC GGGCCTGCCG TCTCTGGGGC TCATAACACC 
ATCATCTGCG CTCGAGCCGG CAAGGACCTC GTCTCCAGCC TCACCTCGGG ACTGCTTACC 
ATTGGCGACC GGTTTGGGGG TGCCCTGGAT GCAGCAGCCA AGATGTTCAG CAAGGCCTTT 
GACAGTGGTA TCATCCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAAGG AAAGCTTATC 
ATGGGCATTG GTCACCGAGT GAAGTCGATC AACAACCCAG ACATGCGCGT GCAGATTCTC 
AAAGATTATG TCAGACAACA CTTCCCTGCC ACCCCACTGC TCGACTACGC ACTGGAAGTA 
GAGAAGATTA CCACTTCCAA GAAACCAAAC CTTATCCTGA ATGTGGACGG TTTAATCGGA 
GTTGCATTTG TAGACATGCT TAGACACTGC GGGTCCTTCA CTCGGGAGGA AGCTGATGAA 
TATATTGACA TTGGAGCCCT CAACGGCATC TTTGTGCTGG GAAGGAGCAT GGGCTTCATT 
GGACACTATC TTGATCAGAA GAGGCTGAAG CAGGGGCTCT ACCGTCACCC ATGGGATGAT 
ATTTCATATG TTCTTCCGGA ACACATGAGC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032534.1
CDS	65..3340
Translation	MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQSLVVKPDQLIKRRGKLGLIGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPFVPHTQEEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYVLDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRTDEVAPAKKAKPAMLQGKSATLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTAGVKMIVVLGEIGGTEEYKICRGVTEGRITKPVVCWCIGTCAAMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVARGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRHCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

Target ORF information:

RefSeq Version	NM_001037457.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP citrate lyase (ACLY), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037457.1

ORF Insert Sequence:

ATGTCGGCCA AGGCGATTTC TGAGCAGACA GGCAAAGAGC TCCTCTACAA GTACATCTGC 
ACCACCTCGG CCATCCAGAA CCGCTTCAAG TATGCCCGGG TCACCCCTGA CACAGACTGG 
GCCCGCCTGT TGCAGGACCA CCCGTGGCTG CTCAGCCAGA GCTTGGTGGT CAAGCCAGAC 
CAGCTGATCA AACGGCGTGG AAAGCTGGGC CTAATTGGGG TCAACCTCAC TCTGGATGGA 
GTCAAGTCCT GGCTGAAGCC ACGCCTGGGA CAGGAAGCTA CAGTTGGCAA GGCCACAGGC 
TTCCTCAAGA ACTTCCTGAT CGAACCCTTC GTCCCCCACA CTCAGGAGGA AGAGTTCTAC 
GTCTGCATCT ATGCTACCCG AGAAGGGGAC TATGTCCTGT TCCACCACGA GGGTGGGGTG 
GACGTGGGCG ACGTGGATGC TAAAGCCCAG AAACTACTTG TCGGCGTCGA TGAGAAGCTG 
AATCCTGAGG ACATCAAGAA ACACCTGCTG GTCCACGCCC CCGAAGACAA GAAAGAAATT 
CTGGCCAGCT TTATCTCCGG CCTCTTCAAT TTCTATGAGG ATCTGTACTT CACCTACCTC 
GAGATCAACC CCCTTGTCGT GACCAAAGAT GGCGTCTACG TCCTTGACTT GGCCGCCAAG 
GTGGATGCCA CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATAGA GTTCCCACCC 
CCCTTCGGAC GGGAGGCTTA CCCAGAGGAA GCCTACATCG CAGATCTGGA CGCCAAAAGT 
GGGGCAAGCT TGAAGCTGAC TTTACTGAAT CCCAAGGGGA GAATCTGGAC CATGGTGGCC 
GGAGGGGGTG CCTCTGTCGT GTACAGTGAC ACCATCTGCG ACCTGGGGGG TGTCAACGAG 
CTGGCAAACT ACGGGGAGTA CTCGGGCGCC CCCAGCGAGC AGCAGACCTA TGACTACGCC 
AAGACGATCC TCTCCCTTAT GACCCGCGAG AAGCACCCAG ATGGCAAGAT CCTCATCATC 
GGAGGCAGCA TCGCAAACTT CACCAACGTG GCTGCCACAT TCAAGGGCAT CGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CGATCTTTGT CCGAAGAGGT 
GGCCCCAACT ATCAGGAGGG CTTACGGGTC ATGGGAGAAG TCGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTCGGCAC TGAGACTCAC ATGACGGCCA TCGTGGGCAT GGCCCTGGGC 
CATCGGCCCA TCCCCAATCA GCCGCCCACA GCGGCCCACA CCGCAAACTT CCTCCTCAAC 
GCCAGCGGGA GTACTTCAAC TCCAGCCCCC AGCAGGACGG CATCTTTTTC TGAGTCCAGA 
ACTGATGAGG TGGCCCCTGC CAAGAAGGCC AAGCCTGCCA TGCTCCAAGG AAAGAGCGCC 
ACCCTCTTCA GCCGCCACAC CAAGGCCATC GTGTGGGGCA TGCAGACCCG GGCTGTGCAG 
GGCATGCTGG ACTTCGACTA CGTCTGCTCC CGCGACGAGC CCTCAGTGGC TGCCATGGTC 
TACCCGTTCA CTGGGGACCA CAAACAGAAG TTTTACTGGG GTCACAAGGA GATCCTGATC 
CCCGTCTTTA AGAACATGGC TGATGCCATG AAGAAGCACC CAGAGGTAGA CGTGCTAATC 
AACTTTGCCT CCCTGCGCTC AGCCTACGAC AGCACCATGG AGACCATGAA TTACGCTCAG 
ATCCGGACCA TCGCCATCAT AGCCGAAGGC ATCCCTGAGG CGCTCACGAG GAAGCTGATC 
AAGAAGGCAG ACCAGAAGGG AGTGACCATC ATTGGACCTG CCACCGTTGG TGGCATCAAG 
CCCGGGTGCT TTAAGATCGG GAACACCGGC GGGATGCTGG ACAACATCCT GGCCTCTAAG 
CTGTACCGCC CAGGCAGTGT GGCCTATGTC TCGCGTTCGG GTGGCATGTC CAACGAGCTC 
AACAATATCA TCTCTCGAAC CACAGATGGC GTCTACGAGG GCGTGGCCAT TGGTGGGGAC 
AGGTACCCCG GCTCAACGTT TATGGATCAC GTGTTACGTT ATCAGGACAC TGCAGGAGTC 
AAAATGATTG TGGTTCTTGG AGAGATAGGG GGCACAGAGG AGTATAAGAT CTGCCGGGGC 
GTCACGGAGG GCCGCATCAC CAAGCCCGTG GTCTGCTGGT GCATCGGGAC CTGTGCGGCC 
ATGTTCTCCT CCGAGGTCCA GTTCGGCCAC GCTGGAGCTT GTGCCAACCA GGCTTCTGAA 
ACCGCAGTTG CCAAGAACCA GGCCTTGAAG GAGGCTGGAG TGTTTGTGCC CCGGAGCTTC 
GATGAGCTTG GAGAAATCAT CCAGTCCGTG TACGAGGATC TCGTGGCCAG AGGAGTCATT 
GTGCCTGCTC AGGAGGTTCC GCCCCCCACA GTACCCATGG ACTACTCTTG GGCCCGGGAA 
TTGGGCTTGA TCCGCAAACC TGCCTCGTTC ATGACCAGCA TCTGTGACGA GCGAGGACAG 
GAGCTCATCT ACGCGGGCAT GCCCATCACT GAGGTCTTCA AGGAGGAGAT GGGCATTGGC 
GGGGTCCTCG GCCTCCTCTG GTTCCAGAAA AGGTTGCCCA AATACTCCTG CCAGTTCATT 
GAGATGTGCC TGATGGTGAC GGCTGATCAC GGGCCTGCCG TCTCTGGGGC TCATAACACC 
ATCATCTGCG CTCGAGCCGG CAAGGACCTC GTCTCCAGCC TCACCTCGGG ACTGCTTACC 
ATTGGCGACC GGTTTGGGGG TGCCCTGGAT GCAGCAGCCA AGATGTTCAG CAAGGCCTTT 
GACAGTGGTA TCATCCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAAGG AAAGCTTATC 
ATGGGCATTG GTCACCGAGT GAAGTCGATC AACAACCCAG ACATGCGCGT GCAGATTCTC 
AAAGATTATG TCAGACAACA CTTCCCTGCC ACCCCACTGC TCGACTACGC ACTGGAAGTA 
GAGAAGATTA CCACTTCCAA GAAACCAAAC CTTATCCTGA ATGTGGACGG TTTAATCGGA 
GTTGCATTTG TAGACATGCT TAGACACTGC GGGTCCTTCA CTCGGGAGGA AGCTGATGAA 
TATATTGACA TTGGAGCCCT CAACGGCATC TTTGTGCTGG GAAGGAGCAT GGGCTTCATT 
GGACACTATC TTGATCAGAA GAGGCTGAAG CAGGGGCTCT ACCGTCACCC ATGGGATGAT 
ATTTCATATG TTCTTCCGGA ACACATGAGC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACLY ( XM_005220713.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220770.1 Bos taurus ATP citrate lyase (ACLY), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30016
Clone ID Related Accession (Same CDS sequence)	XM_005220713.3
Accession Version	XM_005220713.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ATP-citrate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCA AGGCGATTTC TGAGCAGACA GGCAAAGAGC TCCTCTACAA GTACATCTGC 
ACCACCTCGG CCATCCAGAA CCGCTTCAAG TATGCCCGGG TCACCCCTGA CACAGACTGG 
GCCCGCCTGT TGCAGGACCA CCCGTGGCTG CTCAGCCAGA GCTTGGTGGT CAAGCCAGAC 
CAGCTGATCA AACGGCGTGG AAAGCTGGGC CTAATTGGGG TCAACCTCAC TCTGGATGGA 
GTCAAGTCCT GGCTGAAGCC ACGCCTGGGA CAGGAAGCTA CAGTTGGCAA GGCCACAGGC 
TTCCTCAAGA ACTTCCTGAT CGAACCCTTC GTCCCCCACA CTCAGGAGGA AGAGTTCTAC 
GTCTGCATCT ATGCTACCCG AGAAGGGGAC TATGTCCTGT TCCACCACGA GGGTGGGGTG 
GACGTGGGCG ACGTGGATGC TAAAGCCCAG AAACTACTTG TCGGCGTCGA TGAGAAGCTG 
AATCCTGAGG ACATCAAGAA ACACCTGCTG GTCCACGCCC CCGAAGACAA GAAAGAAATT 
CTGGCCAGCT TTATCTCCGG CCTCTTCAAT TTCTATGAGG ATCTGTACTT CACCTACCTC 
GAGATCAACC CCCTTGTCGT GACCAAAGAT GGCGTCTACG TCCTTGACTT GGCCGCCAAG 
GTGGATGCCA CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATAGA GTTCCCACCC 
CCCTTCGGAC GGGAGGCTTA CCCAGAGGAA GCCTACATCG CAGATCTGGA CGCCAAAAGT 
GGGGCAAGCT TGAAGCTGAC TTTACTGAAT CCCAAGGGGA GAATCTGGAC CATGGTGGCC 
GGAGGGGGTG CCTCTGTCGT GTACAGTGAC ACCATCTGCG ACCTGGGGGG TGTCAACGAG 
CTGGCAAACT ACGGGGAGTA CTCGGGCGCC CCCAGCGAGC AGCAGACCTA TGACTACGCC 
AAGACGATCC TCTCCCTTAT GACCCGCGAG AAGCACCCAG ATGGCAAGAT CCTCATCATC 
GGAGGCAGCA TCGCAAACTT CACCAACGTG GCTGCCACAT TCAAGGGCAT CGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CGATCTTTGT CCGAAGAGGT 
GGCCCCAACT ATCAGGAGGG CTTACGGGTC ATGGGAGAAG TCGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTCGGCAC CGAGACTCAC ATGACGGCCA TCGTGGGCAT GGCCCTGGGC 
CATCGGCCCA TCCCCAATCA GCCGCCCACA GCGGCCCACA CCGCAAACTT CCTCCTCAAC 
GCCAGCGGGA GTACTTCAAC TCCAGCCCCC AGCAGGACGG CATCTTTTTC TGAGTCCAGA 
ACTGATGAGG TGGCCCCTGC CAAGAAGGCC AAGCCTGCCA TGCTCCAAGC CTCCAGCCAT 
CAACGGGTCA CTCAGTCGCC TTGGTCATTT TCTTTGACAG ATTCAGTCCC AAGTCCAAGA 
CCCCTGCAAG GAAAGAGCGC CACCCTCTTC AGCCGCCACA CCAAGGCCAT CGTGTGGGGC 
ATGCAGACCC GGGCTGTGCA GGGCATGCTG GACTTCGACT ACGTCTGCTC CCGCGACGAG 
CCCTCAGTGG CTGCCATGGT CTACCCGTTC ACTGGGGACC ACAAACAGAA GTTTTACTGG 
GGTCACAAGG AGATCCTGAT CCCCGTCTTT AAGAACATGG CTGATGCCAT GAAGAAGCAC 
CCAGAGGTAG ACGTGCTAAT CAACTTTGCC TCCCTGCGCT CAGCCTACGA CAGCACCATG 
GAGACCATGA ATTACGCTCA GATCCGGACC ATCGCCATCA TAGCCGAAGG CATCCCTGAG 
GCGCTCACGA GGAAGCTGAT CAAGAAGGCA GACCAGAAGG GAGTGACCAT CATTGGACCT 
GCCACCGTTG GTGGCATCAA GCCCGGGTGC TTTAAGATCG GGAACACCGG CGGGATGCTG 
GACAACATCC TGGCCTCTAA GCTGTACCGC CCAGGCAGTG TGGCCTATGT CTCGCGTTCG 
GGTGGCATGT CCAACGAGCT CAACAATATC ATCTCTCGAA CCACAGATGG CGTCTACGAG 
GGCGTGGCCA TTGGTGGGGA CAGGTACCCC GGCTCAACGT TTATGGATCA CGTGTTACGT 
TATCAGGACA CTGCAGGAGT CAAAATGATT GTGGTTCTTG GAGAGATAGG GGGCACAGAG 
GAGTATAAGA TCTGCCGGGG CGTCACGGAG GGCCGCATCA CCAAGCCCGT GGTCTGCTGG 
TGCATCGGGA CCTGTGCGGC CATGTTCTCC TCCGAGGTCC AGTTCGGCCA CGCTGGAGCT 
TGTGCCAACC AGGCTTCTGA AACCGCAGTT GCCAAGAACC AGGCCTTGAA GGAGGCTGGA 
GTGTTTGTGC CCCGGAGCTT CGATGAGCTT GGAGAAATCA TCCAGTCCGT GTACGAGGAT 
CTCGTGGCCA GAGGAGTCAT TGTGCCTGCT CAGGAGGTTC CGCCCCCCAC AGTACCCATG 
GACTACTCTT GGGCCCGGGA ATTGGGCTTG ATCCGCAAAC CTGCCTCGTT CATGACCAGC 
ATCTGTGACG AGCGAGGACA GGAGCTCATC TACGCGGGCA TGCCCATCAC TGAGGTCTTC 
AAGGAGGAGA TGGGCATTGG CGGGGTCCTC GGCCTCCTCT GGTTCCAGAA AAGGTTGCCC 
AAATACTCCT GCCAGTTCAT TGAGATGTGC CTGATGGTGA CGGCTGATCA CGGGCCTGCC 
GTCTCTGGGG CTCATAACAC CATCATCTGC GCTCGAGCCG GCAAGGACCT CGTCTCCAGC 
CTCACCTCGG GACTGCTTAC CATTGGCGAC CGGTTTGGGG GTGCCCTGGA TGCAGCAGCC 
AAGATGTTCA GCAAGGCCTT TGACAGTGGT ATCATCCCCA TGGAGTTTGT GAACAAGATG 
AAGAAGGAAG GAAAGCTTAT CATGGGCATT GGTCACCGAG TGAAGTCGAT CAACAACCCA 
GACATGCGCG TGCAGATTCT CAAAGATTAT GTCAGACAAC ACTTCCCTGC CACCCCACTG 
CTCGACTACG CACTGGAAGT AGAGAAGATT ACCACTTCCA AGAAACCAAA CCTTATCCTG 
AATGTGGACG GTTTAATCGG AGTTGCATTT GTAGACATGC TTAGACACTG CGGGTCCTTC 
ACTCGGGAGG AAGCTGATGA ATATATTGAC ATTGGAGCCC TCAACGGCAT CTTTGTGCTG 
GGAAGGAGCA TGGGCTTCAT TGGACACTAT CTTGATCAGA AGAGGCTGAA GCAGGGGCTC 
TACCGTCACC CATGGGATGA TATTTCATAT GTTCTTCCGG AACACATGAG CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220770.1
CDS	185..3541
Translation	MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQSLVVKPDQLIKRRGKLGLIGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPFVPHTQEEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYVLDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRTDEVAPAKKAKPAMLQASSHQRVTQSPWSFSLTDSVPSPRPLQGKSATLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTAGVKMIVVLGEIGGTEEYKICRGVTEGRITKPVVCWCIGTCAAMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVARGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRHCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

Target ORF information:

RefSeq Version	XM_005220713.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP citrate lyase (ACLY), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220713.3

ORF Insert Sequence:

ATGTCGGCCA AGGCGATTTC TGAGCAGACA GGCAAAGAGC TCCTCTACAA GTACATCTGC 
ACCACCTCGG CCATCCAGAA CCGCTTCAAG TATGCCCGGG TCACCCCTGA CACAGACTGG 
GCCCGCCTGT TGCAGGACCA CCCGTGGCTG CTCAGCCAGA GCTTGGTGGT CAAGCCAGAC 
CAGCTGATCA AACGGCGTGG AAAGCTGGGC CTAATTGGGG TCAACCTCAC TCTGGATGGA 
GTCAAGTCCT GGCTGAAGCC ACGCCTGGGA CAGGAAGCTA CAGTTGGCAA GGCCACAGGC 
TTCCTCAAGA ACTTCCTGAT CGAACCCTTC GTCCCCCACA CTCAGGAGGA AGAGTTCTAC 
GTCTGCATCT ATGCTACCCG AGAAGGGGAC TATGTCCTGT TCCACCACGA GGGTGGGGTG 
GACGTGGGCG ACGTGGATGC TAAAGCCCAG AAACTACTTG TCGGCGTCGA TGAGAAGCTG 
AATCCTGAGG ACATCAAGAA ACACCTGCTG GTCCACGCCC CCGAAGACAA GAAAGAAATT 
CTGGCCAGCT TTATCTCCGG CCTCTTCAAT TTCTATGAGG ATCTGTACTT CACCTACCTC 
GAGATCAACC CCCTTGTCGT GACCAAAGAT GGCGTCTACG TCCTTGACTT GGCCGCCAAG 
GTGGATGCCA CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATAGA GTTCCCACCC 
CCCTTCGGAC GGGAGGCTTA CCCAGAGGAA GCCTACATCG CAGATCTGGA CGCCAAAAGT 
GGGGCAAGCT TGAAGCTGAC TTTACTGAAT CCCAAGGGGA GAATCTGGAC CATGGTGGCC 
GGAGGGGGTG CCTCTGTCGT GTACAGTGAC ACCATCTGCG ACCTGGGGGG TGTCAACGAG 
CTGGCAAACT ACGGGGAGTA CTCGGGCGCC CCCAGCGAGC AGCAGACCTA TGACTACGCC 
AAGACGATCC TCTCCCTTAT GACCCGCGAG AAGCACCCAG ATGGCAAGAT CCTCATCATC 
GGAGGCAGCA TCGCAAACTT CACCAACGTG GCTGCCACAT TCAAGGGCAT CGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CGATCTTTGT CCGAAGAGGT 
GGCCCCAACT ATCAGGAGGG CTTACGGGTC ATGGGAGAAG TCGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTCGGCAC CGAGACTCAC ATGACGGCCA TCGTGGGCAT GGCCCTGGGC 
CATCGGCCCA TCCCCAATCA GCCGCCCACA GCGGCCCACA CCGCAAACTT CCTCCTCAAC 
GCCAGCGGGA GTACTTCAAC TCCAGCCCCC AGCAGGACGG CATCTTTTTC TGAGTCCAGA 
ACTGATGAGG TGGCCCCTGC CAAGAAGGCC AAGCCTGCCA TGCTCCAAGC CTCCAGCCAT 
CAACGGGTCA CTCAGTCGCC TTGGTCATTT TCTTTGACAG ATTCAGTCCC AAGTCCAAGA 
CCCCTGCAAG GAAAGAGCGC CACCCTCTTC AGCCGCCACA CCAAGGCCAT CGTGTGGGGC 
ATGCAGACCC GGGCTGTGCA GGGCATGCTG GACTTCGACT ACGTCTGCTC CCGCGACGAG 
CCCTCAGTGG CTGCCATGGT CTACCCGTTC ACTGGGGACC ACAAACAGAA GTTTTACTGG 
GGTCACAAGG AGATCCTGAT CCCCGTCTTT AAGAACATGG CTGATGCCAT GAAGAAGCAC 
CCAGAGGTAG ACGTGCTAAT CAACTTTGCC TCCCTGCGCT CAGCCTACGA CAGCACCATG 
GAGACCATGA ATTACGCTCA GATCCGGACC ATCGCCATCA TAGCCGAAGG CATCCCTGAG 
GCGCTCACGA GGAAGCTGAT CAAGAAGGCA GACCAGAAGG GAGTGACCAT CATTGGACCT 
GCCACCGTTG GTGGCATCAA GCCCGGGTGC TTTAAGATCG GGAACACCGG CGGGATGCTG 
GACAACATCC TGGCCTCTAA GCTGTACCGC CCAGGCAGTG TGGCCTATGT CTCGCGTTCG 
GGTGGCATGT CCAACGAGCT CAACAATATC ATCTCTCGAA CCACAGATGG CGTCTACGAG 
GGCGTGGCCA TTGGTGGGGA CAGGTACCCC GGCTCAACGT TTATGGATCA CGTGTTACGT 
TATCAGGACA CTGCAGGAGT CAAAATGATT GTGGTTCTTG GAGAGATAGG GGGCACAGAG 
GAGTATAAGA TCTGCCGGGG CGTCACGGAG GGCCGCATCA CCAAGCCCGT GGTCTGCTGG 
TGCATCGGGA CCTGTGCGGC CATGTTCTCC TCCGAGGTCC AGTTCGGCCA CGCTGGAGCT 
TGTGCCAACC AGGCTTCTGA AACCGCAGTT GCCAAGAACC AGGCCTTGAA GGAGGCTGGA 
GTGTTTGTGC CCCGGAGCTT CGATGAGCTT GGAGAAATCA TCCAGTCCGT GTACGAGGAT 
CTCGTGGCCA GAGGAGTCAT TGTGCCTGCT CAGGAGGTTC CGCCCCCCAC AGTACCCATG 
GACTACTCTT GGGCCCGGGA ATTGGGCTTG ATCCGCAAAC CTGCCTCGTT CATGACCAGC 
ATCTGTGACG AGCGAGGACA GGAGCTCATC TACGCGGGCA TGCCCATCAC TGAGGTCTTC 
AAGGAGGAGA TGGGCATTGG CGGGGTCCTC GGCCTCCTCT GGTTCCAGAA AAGGTTGCCC 
AAATACTCCT GCCAGTTCAT TGAGATGTGC CTGATGGTGA CGGCTGATCA CGGGCCTGCC 
GTCTCTGGGG CTCATAACAC CATCATCTGC GCTCGAGCCG GCAAGGACCT CGTCTCCAGC 
CTCACCTCGG GACTGCTTAC CATTGGCGAC CGGTTTGGGG GTGCCCTGGA TGCAGCAGCC 
AAGATGTTCA GCAAGGCCTT TGACAGTGGT ATCATCCCCA TGGAGTTTGT GAACAAGATG 
AAGAAGGAAG GAAAGCTTAT CATGGGCATT GGTCACCGAG TGAAGTCGAT CAACAACCCA 
GACATGCGCG TGCAGATTCT CAAAGATTAT GTCAGACAAC ACTTCCCTGC CACCCCACTG 
CTCGACTACG CACTGGAAGT AGAGAAGATT ACCACTTCCA AGAAACCAAA CCTTATCCTG 
AATGTGGACG GTTTAATCGG AGTTGCATTT GTAGACATGC TTAGACACTG CGGGTCCTTC 
ACTCGGGAGG AAGCTGATGA ATATATTGAC ATTGGAGCCC TCAACGGCAT CTTTGTGCTG 
GGAAGGAGCA TGGGCTTCAT TGGACACTAT CTTGATCAGA AGAGGCTGAA GCAGGGGCTC 
TACCGTCACC CATGGGATGA TATTTCATAT GTTCTTCCGG AACACATGAG CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACLY ( XM_005220715.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220772.1 Bos taurus ATP citrate lyase (ACLY), transcript variant X2, mRNA.

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CloneID	OBa30017
Clone ID Related Accession (Same CDS sequence)	XM_005220715.3
Accession Version	XM_005220715.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ATP-citrate synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220715.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCA AGGCGATTTC TGAGCAGACA GGCAAAGAGC TCCTCTACAA GTACATCTGC 
ACCACCTCGG CCATCCAGAA CCGCTTCAAG TATGCCCGGG TCACCCCTGA CACAGACTGG 
GCCCGCCTGT TGCAGGACCA CCCGTGGCTG CTCAGCCAGA GCTTGGTGGT CAAGCCAGAC 
CAGCTGATCA AACGGCGTGG AAAGCTGGGC CTAATTGGGG TCAACCTCAC TCTGGATGGA 
GTCAAGTCCT GGCTGAAGCC ACGCCTGGGA CAGGAAGCTA CAGTTGGCAA GGCCACAGGC 
TTCCTCAAGA ACTTCCTGAT CGAACCCTTC GTCCCCCACA CTCAGGAGGA AGAGTTCTAC 
GTCTGCATCT ATGCTACCCG AGAAGGGGAC TATGTCCTGT TCCACCACGA GGGTGGGGTG 
GACGTGGGCG ACGTGGATGC TAAAGCCCAG AAACTACTTG TCGGCGTCGA TGAGAAGCTG 
AATCCTGAGG ACATCAAGAA ACACCTGCTG GTCCACGCCC CCGAAGACAA GAAAGAAATT 
CTGGCCAGCT TTATCTCCGG CCTCTTCAAT TTCTATGAGG ATCTGTACTT CACCTACCTC 
GAGATCAACC CCCTTGTCGT GACCAAAGAT GGCGTCTACG TCCTTGACTT GGCCGCCAAG 
GTGGATGCCA CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATAGA GTTCCCACCC 
CCCTTCGGAC GGGAGGCTTA CCCAGAGGAA GCCTACATCG CAGATCTGGA CGCCAAAAGT 
GGGGCAAGCT TGAAGCTGAC TTTACTGAAT CCCAAGGGGA GAATCTGGAC CATGGTGGCC 
GGAGGGGGTG CCTCTGTCGT GTACAGTGAC ACCATCTGCG ACCTGGGGGG TGTCAACGAG 
CTGGCAAACT ACGGGGAGTA CTCGGGCGCC CCCAGCGAGC AGCAGACCTA TGACTACGCC 
AAGACGATCC TCTCCCTTAT GACCCGCGAG AAGCACCCAG ATGGCAAGAT CCTCATCATC 
GGAGGCAGCA TCGCAAACTT CACCAACGTG GCTGCCACAT TCAAGGGCAT CGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CGATCTTTGT CCGAAGAGGT 
GGCCCCAACT ATCAGGAGGG CTTACGGGTC ATGGGAGAAG TCGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTCGGCAC CGAGACTCAC ATGACGGCCA TCGTGGGCAT GGCCCTGGGC 
CATCGGCCCA TCCCCAATCA GCCGCCCACA GCGGCCCACA CCGCAAACTT CCTCCTCAAC 
GCCAGCGGGA GTACTTCAAC TCCAGCCCCC AGCAGGACGG CATCTTTTTC TGAGTCCAGA 
ACTGATGAGG TGGCCCCTGC CAAGAAGGCC AAGCCTGCCA TGCTCCAAGA TTCAGTCCCA 
AGTCCAAGAC CCCTGCAAGG AAAGAGCGCC ACCCTCTTCA GCCGCCACAC CAAGGCCATC 
GTGTGGGGCA TGCAGACCCG GGCTGTGCAG GGCATGCTGG ACTTCGACTA CGTCTGCTCC 
CGCGACGAGC CCTCAGTGGC TGCCATGGTC TACCCGTTCA CTGGGGACCA CAAACAGAAG 
TTTTACTGGG GTCACAAGGA GATCCTGATC CCCGTCTTTA AGAACATGGC TGATGCCATG 
AAGAAGCACC CAGAGGTAGA CGTGCTAATC AACTTTGCCT CCCTGCGCTC AGCCTACGAC 
AGCACCATGG AGACCATGAA TTACGCTCAG ATCCGGACCA TCGCCATCAT AGCCGAAGGC 
ATCCCTGAGG CGCTCACGAG GAAGCTGATC AAGAAGGCAG ACCAGAAGGG AGTGACCATC 
ATTGGACCTG CCACCGTTGG TGGCATCAAG CCCGGGTGCT TTAAGATCGG GAACACCGGC 
GGGATGCTGG ACAACATCCT GGCCTCTAAG CTGTACCGCC CAGGCAGTGT GGCCTATGTC 
TCGCGTTCGG GTGGCATGTC CAACGAGCTC AACAATATCA TCTCTCGAAC CACAGATGGC 
GTCTACGAGG GCGTGGCCAT TGGTGGGGAC AGGTACCCCG GCTCAACGTT TATGGATCAC 
GTGTTACGTT ATCAGGACAC TGCAGGAGTC AAAATGATTG TGGTTCTTGG AGAGATAGGG 
GGCACAGAGG AGTATAAGAT CTGCCGGGGC GTCACGGAGG GCCGCATCAC CAAGCCCGTG 
GTCTGCTGGT GCATCGGGAC CTGTGCGGCC ATGTTCTCCT CCGAGGTCCA GTTCGGCCAC 
GCTGGAGCTT GTGCCAACCA GGCTTCTGAA ACCGCAGTTG CCAAGAACCA GGCCTTGAAG 
GAGGCTGGAG TGTTTGTGCC CCGGAGCTTC GATGAGCTTG GAGAAATCAT CCAGTCCGTG 
TACGAGGATC TCGTGGCCAG AGGAGTCATT GTGCCTGCTC AGGAGGTTCC GCCCCCCACA 
GTACCCATGG ACTACTCTTG GGCCCGGGAA TTGGGCTTGA TCCGCAAACC TGCCTCGTTC 
ATGACCAGCA TCTGTGACGA GCGAGGACAG GAGCTCATCT ACGCGGGCAT GCCCATCACT 
GAGGTCTTCA AGGAGGAGAT GGGCATTGGC GGGGTCCTCG GCCTCCTCTG GTTCCAGAAA 
AGGTTGCCCA AATACTCCTG CCAGTTCATT GAGATGTGCC TGATGGTGAC GGCTGATCAC 
GGGCCTGCCG TCTCTGGGGC TCATAACACC ATCATCTGCG CTCGAGCCGG CAAGGACCTC 
GTCTCCAGCC TCACCTCGGG ACTGCTTACC ATTGGCGACC GGTTTGGGGG TGCCCTGGAT 
GCAGCAGCCA AGATGTTCAG CAAGGCCTTT GACAGTGGTA TCATCCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG AAAGCTTATC ATGGGCATTG GTCACCGAGT GAAGTCGATC 
AACAACCCAG ACATGCGCGT GCAGATTCTC AAAGATTATG TCAGACAACA CTTCCCTGCC 
ACCCCACTGC TCGACTACGC ACTGGAAGTA GAGAAGATTA CCACTTCCAA GAAACCAAAC 
CTTATCCTGA ATGTGGACGG TTTAATCGGA GTTGCATTTG TAGACATGCT TAGACACTGC 
GGGTCCTTCA CTCGGGAGGA AGCTGATGAA TATATTGACA TTGGAGCCCT CAACGGCATC 
TTTGTGCTGG GAAGGAGCAT GGGCTTCATT GGACACTATC TTGATCAGAA GAGGCTGAAG 
CAGGGGCTCT ACCGTCACCC ATGGGATGAT ATTTCATATG TTCTTCCGGA ACACATGAGC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220772.1
CDS	184..3489
Translation	MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQSLVVKPDQLIKRRGKLGLIGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPFVPHTQEEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYVLDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRTDEVAPAKKAKPAMLQDSVPSPRPLQGKSATLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTAGVKMIVVLGEIGGTEEYKICRGVTEGRITKPVVCWCIGTCAAMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVARGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRHCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM

Target ORF information:

RefSeq Version	XM_005220715.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP citrate lyase (ACLY), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220715.3

ORF Insert Sequence:

ATGTCGGCCA AGGCGATTTC TGAGCAGACA GGCAAAGAGC TCCTCTACAA GTACATCTGC 
ACCACCTCGG CCATCCAGAA CCGCTTCAAG TATGCCCGGG TCACCCCTGA CACAGACTGG 
GCCCGCCTGT TGCAGGACCA CCCGTGGCTG CTCAGCCAGA GCTTGGTGGT CAAGCCAGAC 
CAGCTGATCA AACGGCGTGG AAAGCTGGGC CTAATTGGGG TCAACCTCAC TCTGGATGGA 
GTCAAGTCCT GGCTGAAGCC ACGCCTGGGA CAGGAAGCTA CAGTTGGCAA GGCCACAGGC 
TTCCTCAAGA ACTTCCTGAT CGAACCCTTC GTCCCCCACA CTCAGGAGGA AGAGTTCTAC 
GTCTGCATCT ATGCTACCCG AGAAGGGGAC TATGTCCTGT TCCACCACGA GGGTGGGGTG 
GACGTGGGCG ACGTGGATGC TAAAGCCCAG AAACTACTTG TCGGCGTCGA TGAGAAGCTG 
AATCCTGAGG ACATCAAGAA ACACCTGCTG GTCCACGCCC CCGAAGACAA GAAAGAAATT 
CTGGCCAGCT TTATCTCCGG CCTCTTCAAT TTCTATGAGG ATCTGTACTT CACCTACCTC 
GAGATCAACC CCCTTGTCGT GACCAAAGAT GGCGTCTACG TCCTTGACTT GGCCGCCAAG 
GTGGATGCCA CTGCCGACTA CATCTGCAAA GTGAAGTGGG GTGACATAGA GTTCCCACCC 
CCCTTCGGAC GGGAGGCTTA CCCAGAGGAA GCCTACATCG CAGATCTGGA CGCCAAAAGT 
GGGGCAAGCT TGAAGCTGAC TTTACTGAAT CCCAAGGGGA GAATCTGGAC CATGGTGGCC 
GGAGGGGGTG CCTCTGTCGT GTACAGTGAC ACCATCTGCG ACCTGGGGGG TGTCAACGAG 
CTGGCAAACT ACGGGGAGTA CTCGGGCGCC CCCAGCGAGC AGCAGACCTA TGACTACGCC 
AAGACGATCC TCTCCCTTAT GACCCGCGAG AAGCACCCAG ATGGCAAGAT CCTCATCATC 
GGAGGCAGCA TCGCAAACTT CACCAACGTG GCTGCCACAT TCAAGGGCAT CGTGAGAGCA 
ATTCGAGATT ACCAGGGTCC CCTGAAGGAG CACGAGGTCA CGATCTTTGT CCGAAGAGGT 
GGCCCCAACT ATCAGGAGGG CTTACGGGTC ATGGGAGAAG TCGGGAAGAC CACTGGGATC 
CCCATCCATG TCTTCGGCAC CGAGACTCAC ATGACGGCCA TCGTGGGCAT GGCCCTGGGC 
CATCGGCCCA TCCCCAATCA GCCGCCCACA GCGGCCCACA CCGCAAACTT CCTCCTCAAC 
GCCAGCGGGA GTACTTCAAC TCCAGCCCCC AGCAGGACGG CATCTTTTTC TGAGTCCAGA 
ACTGATGAGG TGGCCCCTGC CAAGAAGGCC AAGCCTGCCA TGCTCCAAGA TTCAGTCCCA 
AGTCCAAGAC CCCTGCAAGG AAAGAGCGCC ACCCTCTTCA GCCGCCACAC CAAGGCCATC 
GTGTGGGGCA TGCAGACCCG GGCTGTGCAG GGCATGCTGG ACTTCGACTA CGTCTGCTCC 
CGCGACGAGC CCTCAGTGGC TGCCATGGTC TACCCGTTCA CTGGGGACCA CAAACAGAAG 
TTTTACTGGG GTCACAAGGA GATCCTGATC CCCGTCTTTA AGAACATGGC TGATGCCATG 
AAGAAGCACC CAGAGGTAGA CGTGCTAATC AACTTTGCCT CCCTGCGCTC AGCCTACGAC 
AGCACCATGG AGACCATGAA TTACGCTCAG ATCCGGACCA TCGCCATCAT AGCCGAAGGC 
ATCCCTGAGG CGCTCACGAG GAAGCTGATC AAGAAGGCAG ACCAGAAGGG AGTGACCATC 
ATTGGACCTG CCACCGTTGG TGGCATCAAG CCCGGGTGCT TTAAGATCGG GAACACCGGC 
GGGATGCTGG ACAACATCCT GGCCTCTAAG CTGTACCGCC CAGGCAGTGT GGCCTATGTC 
TCGCGTTCGG GTGGCATGTC CAACGAGCTC AACAATATCA TCTCTCGAAC CACAGATGGC 
GTCTACGAGG GCGTGGCCAT TGGTGGGGAC AGGTACCCCG GCTCAACGTT TATGGATCAC 
GTGTTACGTT ATCAGGACAC TGCAGGAGTC AAAATGATTG TGGTTCTTGG AGAGATAGGG 
GGCACAGAGG AGTATAAGAT CTGCCGGGGC GTCACGGAGG GCCGCATCAC CAAGCCCGTG 
GTCTGCTGGT GCATCGGGAC CTGTGCGGCC ATGTTCTCCT CCGAGGTCCA GTTCGGCCAC 
GCTGGAGCTT GTGCCAACCA GGCTTCTGAA ACCGCAGTTG CCAAGAACCA GGCCTTGAAG 
GAGGCTGGAG TGTTTGTGCC CCGGAGCTTC GATGAGCTTG GAGAAATCAT CCAGTCCGTG 
TACGAGGATC TCGTGGCCAG AGGAGTCATT GTGCCTGCTC AGGAGGTTCC GCCCCCCACA 
GTACCCATGG ACTACTCTTG GGCCCGGGAA TTGGGCTTGA TCCGCAAACC TGCCTCGTTC 
ATGACCAGCA TCTGTGACGA GCGAGGACAG GAGCTCATCT ACGCGGGCAT GCCCATCACT 
GAGGTCTTCA AGGAGGAGAT GGGCATTGGC GGGGTCCTCG GCCTCCTCTG GTTCCAGAAA 
AGGTTGCCCA AATACTCCTG CCAGTTCATT GAGATGTGCC TGATGGTGAC GGCTGATCAC 
GGGCCTGCCG TCTCTGGGGC TCATAACACC ATCATCTGCG CTCGAGCCGG CAAGGACCTC 
GTCTCCAGCC TCACCTCGGG ACTGCTTACC ATTGGCGACC GGTTTGGGGG TGCCCTGGAT 
GCAGCAGCCA AGATGTTCAG CAAGGCCTTT GACAGTGGTA TCATCCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG AAAGCTTATC ATGGGCATTG GTCACCGAGT GAAGTCGATC 
AACAACCCAG ACATGCGCGT GCAGATTCTC AAAGATTATG TCAGACAACA CTTCCCTGCC 
ACCCCACTGC TCGACTACGC ACTGGAAGTA GAGAAGATTA CCACTTCCAA GAAACCAAAC 
CTTATCCTGA ATGTGGACGG TTTAATCGGA GTTGCATTTG TAGACATGCT TAGACACTGC 
GGGTCCTTCA CTCGGGAGGA AGCTGATGAA TATATTGACA TTGGAGCCCT CAACGGCATC 
TTTGTGCTGG GAAGGAGCAT GGGCTTCATT GGACACTATC TTGATCAGAA GAGGCTGAAG 
CAGGGGCTCT ACCGTCACCC ATGGGATGAT ATTTCATATG TTCTTCCGGA ACACATGAGC 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association of genetic variations in the ACLY gene with growth traits in Chinese beef cattle.
Genetics and molecular research : GMR15(2)(2016 Jul)
Li MN,Guo X,Bao PJ,Wu XY,Ding XZ,Chu M,Liang CN,Yan P

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ACLY cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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