acly cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	acly
Entrez Gene ID	493390
Full Name	ATP citrate lyase
General protein information	Preferred Names ATP citrate lyase Names ATP-citrate synthase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008027.1	NP_001008028.1	ATP-citrate synthase
XM_012971672.2	XP_012827126.1	ATP-citrate synthase isoform X1
XM_012971672.3	XP_012827126.1	ATP-citrate synthase isoform X1
XM_012971671.2	XP_012827125.1	ATP-citrate synthase isoform X1
XM_012971671.3	XP_012827125.1	ATP-citrate synthase isoform X1
XM_012971673.3	XP_012827127.1	ATP-citrate synthase isoform X2
XM_012971673.2	XP_012827127.1	ATP-citrate synthase isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Acly	NP_001186225.1
Gallus gallus (chicken)	ACLY	NP_001025711.1
Danio rerio (zebrafish)	aclya	NP_001002649.1
Caenorhabditis elegans (roundworm)	acly-2	NP_506267.1
Magnaporthe oryzae (rice blast fungus)	MGG_06719	XP_003709430.1
Arabidopsis thaliana (thale cress)	ACLB-2	NP_199757.1
Arabidopsis thaliana (thale cress)	ACLB-1	NP_187317.1
Caenorhabditis elegans (roundworm)	D1005.1	NP_508280.1
Rattus norvegicus (Norway rat)	Acly	NP_058683.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010156	XP_319323.3
Oryza sativa (rice)	Os01g0300200	NP_001042817.1
Xenopus tropicalis (tropical clawed frog)	acly	NP_001008028.1
	ACLY	XP_001108114.2
Bos taurus (cattle)	ACLY	NP_001032534.1
Homo sapiens (human)	ACLY	NP_001087.2
Pan troglodytes (chimpanzee)	ACLY	XP_511495.3
Canis lupus familiaris (dog)	ACLY	XP_850198.1
Drosophila melanogaster (fruit fly)	ATPCL	NP_725514.2
Schizosaccharomyces pombe (fission yeast)	SPBC1703.07	NP_596202.1
Neurospora crassa	NCU06785	XP_963626.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following acly gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acly cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa26918	XM_012971671.3 Latest version!	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa26918	XM_012971672.3 Latest version!	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa26919	XM_012971673.3 Latest version!	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa01580	NM_001008027.1 Latest version!	Xenopus tropicalis ATP citrate lyase (acly), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa26918	XM_012971672.2	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa26918	XM_012971671.2	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa26919	XM_012971673.2	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

acly ( XM_012971672.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827126.1 Xenopus tropicalis ATP citrate lyase (acly), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa26918
Clone ID Related Accession (Same CDS sequence)	XM_012971672.2 , XM_012971672.3 , XM_012971671.2 , XM_012971671.3
Accession Version	XM_012971672.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-citrate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971672.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGA TTCTACAGCC 
TTGCACGGAC CGGCTACAGC CAAAGCCACC ACTCTGTTCA ACCGTCACAC CAAATCCATC 
GTTTGGGGCA TGCAGACCCG AGCCGTGCAG GGCATGCTGG ACTTCGACTA CATCTGCTCC 
CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT TACCCTTTCA CTGGTGATCA CAAGCAAAAG 
TTTTACTGGG GCCACAAAGA GATCCTCATA CCGGTCTTCA AAAACATGGC GGATGCGATG 
AGGAAACACC CCGAGGTGGA CGTGCTCATT AACTTTGCCT CCCTGAGGTC GGCGTATGAC 
AGCACCATCG AGACCATGAA TTACCCGCAG ATCCGTTCAA TTGCAATTAT TGCAGAAGGA 
ATACCAGAAG CACTTACAAG GAAGCTCATC AAGATGGCGG ATGAGAAAGG AGTCACAATT 
ATAGGTCCTG CCACGGTTGG TGGAATTAAA CCTGGTTGCT TTAAGATTGG AAACACAGGG 
GGAATGCTGG ACAATATTCT GGCCTCTAAG CTCTACCGCC CTGGGAGCGT CGCCTATGTG 
TCCCGCTCAG GGGGAATGTC CAACGAGCTC AACAACATCA TCTCCAGGAC CACGGATGGA 
GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC AGATACCCCG GGTCAACATT CATGGACCAT 
GTTCTACGTT ACCAGGACAC TCCAGGAGTT AAAATGATTG TTGTTCTTGG AGAGATTGGG 
GGCACGGAGG AGTACAAGAT CTGCCGTGGT ATTAAGGAAG GCCGGCTGAC TAAACCAATC 
GTCTGTTGGT GTATTGGGAC CTGCGCTACT ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT 
GCAGGAGCTT GTGCCAACCA AGCATCGGAG ACGGCTGTGG CTAAAAACCA GGCCTTGAAG 
GAAGCTGGAG TATTCGTTCC ACGTAGTTTT GATGAACTGG GGGATGTCAT TCAGTCTGTG 
TATGAGGACC TAGTTGCTAA AGGAGAAATT GTACCAGCTG AGGAAGTGCC ACCTCCCACT 
GTACCCATGG ACTACTCCTG GGCGAGGGAG CTGGGTTTGA TCCGGAAGCC TGCATCTTTC 
ATGACTAGTA TTTGTGACGA GAGGGGCCAG GAGCTGATCT ACGCAGGAAT GCCAATCACT 
GATGTCTTCA AGGAAGATAT TGGCATCGGA GGAGTATTGG GCCTGCTGTG GTTCCAGAGA 
AGGATGCCCA AGTATGCCTC TCACTTTATT GAGATGTGCC TGATGGTGAC GGCAGACCAC 
GGGCCGGCTG TGTCGGGGGC CCATAATACC ATTGTGTGTG CCAGAGCCGG AAAAGACCTG 
ATCTCTAGCC TGACGTCTGG ACTGCTTACA ATTGGCGATC GCTTCGGGGG AGCACTTGAT 
GCCGCTGCCA AGATGTTCAG CAAAGCGTTT GACAGCGGCC TTATTCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG GAAGCTGATC ATGGGCATCG GCCACAGAGT CAAATCTATC 
AACAACCCAG ACATGAGAGT ACAGATCCTG AAGGACTACG TCAAGCAGAA CTTCCCGGCC 
ACTCCTTTAC TGGACTATGC CCTCGAGGTG GAAAAGATCA CAACTTCAAA GAAACCAAAC 
CTCATCCTTA ACGTTGATGG CCTTATCGGT GTGGCGTTTG TAGATCTGCT CAGGACCTGC 
GGCACTTTCA CACGGGAGGA AGCGGATGAA TACGTAGATA TTGGAGCACT GAACGGCATC 
TTTGTTTTGG GAAGAAGCAT GGGTTTTATC GGGCATTACT TGGACCAGAA AAGGCTGAAG 
CAGGGATTGT ACCGGCATCC ATGGGACGAT ATTTCCTACG TTCTGCCTGA GCACATGACT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827126.1
CDS	147..3452
Translation	MSAKAISEQTGKEFLYKYICTTAAVQNRFKYAHVTYDTDWDRLAQEHPWLLTQPLVVKPDQLIKRRGKLGLVGVNLNLDQVKSWLKPRLGNETTIAKAKGILKNFLIEPFVPHKQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDSKAQKLLVGVGERLTEKEVKSRLLIHVAQDKQEVLASFITGLFNLYEDLYFTYLEINPLVVTKDGVYVLDMAAKIDATADYICKAKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPRGRIWTMVAGGGASVVYSDTICDLGGVDELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIKDFQCPLKEHEVMIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSSSTPAPSRTASFSESRTEDITPAKKSKPAIPNDSTALHGPATAKATTLFNRHTKSIVWGMQTRAVQGMLDFDYICSRSEPSVAALVYPFTGDHKQKFYWGHKEILIPVFKNMADAMRKHPEVDVLINFASLRSAYDSTIETMNYPQIRSIAIIAEGIPEALTRKLIKMADEKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVFEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGDVIQSVYEDLVAKGEIVPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITDVFKEDIGIGGVLGLLWFQRRMPKYASHFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGLIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQNFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDLLRTCGTFTREEADEYVDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	XM_012971672.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971672.3

ORF Insert Sequence:

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGA TTCTACAGCC 
TTGCACGGAC CGGCTACAGC CAAAGCCACC ACTCTGTTCA ACCGTCACAC CAAATCCATC 
GTTTGGGGCA TGCAGACCCG AGCCGTGCAG GGCATGCTGG ACTTCGACTA CATCTGCTCC 
CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT TACCCTTTCA CTGGTGATCA CAAGCAAAAG 
TTTTACTGGG GCCACAAAGA GATCCTCATA CCGGTCTTCA AAAACATGGC GGATGCGATG 
AGGAAACACC CCGAGGTGGA CGTGCTCATT AACTTTGCCT CCCTGAGGTC GGCGTATGAC 
AGCACCATCG AGACCATGAA TTACCCGCAG ATCCGTTCAA TTGCAATTAT TGCAGAAGGA 
ATACCAGAAG CACTTACAAG GAAGCTCATC AAGATGGCGG ATGAGAAAGG AGTCACAATT 
ATAGGTCCTG CCACGGTTGG TGGAATTAAA CCTGGTTGCT TTAAGATTGG AAACACAGGG 
GGAATGCTGG ACAATATTCT GGCCTCTAAG CTCTACCGCC CTGGGAGCGT CGCCTATGTG 
TCCCGCTCAG GGGGAATGTC CAACGAGCTC AACAACATCA TCTCCAGGAC CACGGATGGA 
GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC AGATACCCCG GGTCAACATT CATGGACCAT 
GTTCTACGTT ACCAGGACAC TCCAGGAGTT AAAATGATTG TTGTTCTTGG AGAGATTGGG 
GGCACGGAGG AGTACAAGAT CTGCCGTGGT ATTAAGGAAG GCCGGCTGAC TAAACCAATC 
GTCTGTTGGT GTATTGGGAC CTGCGCTACT ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT 
GCAGGAGCTT GTGCCAACCA AGCATCGGAG ACGGCTGTGG CTAAAAACCA GGCCTTGAAG 
GAAGCTGGAG TATTCGTTCC ACGTAGTTTT GATGAACTGG GGGATGTCAT TCAGTCTGTG 
TATGAGGACC TAGTTGCTAA AGGAGAAATT GTACCAGCTG AGGAAGTGCC ACCTCCCACT 
GTACCCATGG ACTACTCCTG GGCGAGGGAG CTGGGTTTGA TCCGGAAGCC TGCATCTTTC 
ATGACTAGTA TTTGTGACGA GAGGGGCCAG GAGCTGATCT ACGCAGGAAT GCCAATCACT 
GATGTCTTCA AGGAAGATAT TGGCATCGGA GGAGTATTGG GCCTGCTGTG GTTCCAGAGA 
AGGATGCCCA AGTATGCCTC TCACTTTATT GAGATGTGCC TGATGGTGAC GGCAGACCAC 
GGGCCGGCTG TGTCGGGGGC CCATAATACC ATTGTGTGTG CCAGAGCCGG AAAAGACCTG 
ATCTCTAGCC TGACGTCTGG ACTGCTTACA ATTGGCGATC GCTTCGGGGG AGCACTTGAT 
GCCGCTGCCA AGATGTTCAG CAAAGCGTTT GACAGCGGCC TTATTCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG GAAGCTGATC ATGGGCATCG GCCACAGAGT CAAATCTATC 
AACAACCCAG ACATGAGAGT ACAGATCCTG AAGGACTACG TCAAGCAGAA CTTCCCGGCC 
ACTCCTTTAC TGGACTATGC CCTCGAGGTG GAAAAGATCA CAACTTCAAA GAAACCAAAC 
CTCATCCTTA ACGTTGATGG CCTTATCGGT GTGGCGTTTG TAGATCTGCT CAGGACCTGC 
GGCACTTTCA CACGGGAGGA AGCGGATGAA TACGTAGATA TTGGAGCACT GAACGGCATC 
TTTGTTTTGG GAAGAAGCAT GGGTTTTATC GGGCATTACT TGGACCAGAA AAGGCTGAAG 
CAGGGATTGT ACCGGCATCC ATGGGACGAT ATTTCCTACG TTCTGCCTGA GCACATGACT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acly ( XM_012971671.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827125.1 Xenopus tropicalis ATP citrate lyase (acly), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa26918
Clone ID Related Accession (Same CDS sequence)	XM_012971672.2 , XM_012971672.3 , XM_012971671.2 , XM_012971671.3
Accession Version	XM_012971671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-citrate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGA TTCTACAGCC 
TTGCACGGAC CGGCTACAGC CAAAGCCACC ACTCTGTTCA ACCGTCACAC CAAATCCATC 
GTTTGGGGCA TGCAGACCCG AGCCGTGCAG GGCATGCTGG ACTTCGACTA CATCTGCTCC 
CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT TACCCTTTCA CTGGTGATCA CAAGCAAAAG 
TTTTACTGGG GCCACAAAGA GATCCTCATA CCGGTCTTCA AAAACATGGC GGATGCGATG 
AGGAAACACC CCGAGGTGGA CGTGCTCATT AACTTTGCCT CCCTGAGGTC GGCGTATGAC 
AGCACCATCG AGACCATGAA TTACCCGCAG ATCCGTTCAA TTGCAATTAT TGCAGAAGGA 
ATACCAGAAG CACTTACAAG GAAGCTCATC AAGATGGCGG ATGAGAAAGG AGTCACAATT 
ATAGGTCCTG CCACGGTTGG TGGAATTAAA CCTGGTTGCT TTAAGATTGG AAACACAGGG 
GGAATGCTGG ACAATATTCT GGCCTCTAAG CTCTACCGCC CTGGGAGCGT CGCCTATGTG 
TCCCGCTCAG GGGGAATGTC CAACGAGCTC AACAACATCA TCTCCAGGAC CACGGATGGA 
GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC AGATACCCCG GGTCAACATT CATGGACCAT 
GTTCTACGTT ACCAGGACAC TCCAGGAGTT AAAATGATTG TTGTTCTTGG AGAGATTGGG 
GGCACGGAGG AGTACAAGAT CTGCCGTGGT ATTAAGGAAG GCCGGCTGAC TAAACCAATC 
GTCTGTTGGT GTATTGGGAC CTGCGCTACT ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT 
GCAGGAGCTT GTGCCAACCA AGCATCGGAG ACGGCTGTGG CTAAAAACCA GGCCTTGAAG 
GAAGCTGGAG TATTCGTTCC ACGTAGTTTT GATGAACTGG GGGATGTCAT TCAGTCTGTG 
TATGAGGACC TAGTTGCTAA AGGAGAAATT GTACCAGCTG AGGAAGTGCC ACCTCCCACT 
GTACCCATGG ACTACTCCTG GGCGAGGGAG CTGGGTTTGA TCCGGAAGCC TGCATCTTTC 
ATGACTAGTA TTTGTGACGA GAGGGGCCAG GAGCTGATCT ACGCAGGAAT GCCAATCACT 
GATGTCTTCA AGGAAGATAT TGGCATCGGA GGAGTATTGG GCCTGCTGTG GTTCCAGAGA 
AGGATGCCCA AGTATGCCTC TCACTTTATT GAGATGTGCC TGATGGTGAC GGCAGACCAC 
GGGCCGGCTG TGTCGGGGGC CCATAATACC ATTGTGTGTG CCAGAGCCGG AAAAGACCTG 
ATCTCTAGCC TGACGTCTGG ACTGCTTACA ATTGGCGATC GCTTCGGGGG AGCACTTGAT 
GCCGCTGCCA AGATGTTCAG CAAAGCGTTT GACAGCGGCC TTATTCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG GAAGCTGATC ATGGGCATCG GCCACAGAGT CAAATCTATC 
AACAACCCAG ACATGAGAGT ACAGATCCTG AAGGACTACG TCAAGCAGAA CTTCCCGGCC 
ACTCCTTTAC TGGACTATGC CCTCGAGGTG GAAAAGATCA CAACTTCAAA GAAACCAAAC 
CTCATCCTTA ACGTTGATGG CCTTATCGGT GTGGCGTTTG TAGATCTGCT CAGGACCTGC 
GGCACTTTCA CACGGGAGGA AGCGGATGAA TACGTAGATA TTGGAGCACT GAACGGCATC 
TTTGTTTTGG GAAGAAGCAT GGGTTTTATC GGGCATTACT TGGACCAGAA AAGGCTGAAG 
CAGGGATTGT ACCGGCATCC ATGGGACGAT ATTTCCTACG TTCTGCCTGA GCACATGACT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827125.1
CDS	159..3464
Translation	MSAKAISEQTGKEFLYKYICTTAAVQNRFKYAHVTYDTDWDRLAQEHPWLLTQPLVVKPDQLIKRRGKLGLVGVNLNLDQVKSWLKPRLGNETTIAKAKGILKNFLIEPFVPHKQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDSKAQKLLVGVGERLTEKEVKSRLLIHVAQDKQEVLASFITGLFNLYEDLYFTYLEINPLVVTKDGVYVLDMAAKIDATADYICKAKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPRGRIWTMVAGGGASVVYSDTICDLGGVDELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIKDFQCPLKEHEVMIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSSSTPAPSRTASFSESRTEDITPAKKSKPAIPNDSTALHGPATAKATTLFNRHTKSIVWGMQTRAVQGMLDFDYICSRSEPSVAALVYPFTGDHKQKFYWGHKEILIPVFKNMADAMRKHPEVDVLINFASLRSAYDSTIETMNYPQIRSIAIIAEGIPEALTRKLIKMADEKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVFEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGDVIQSVYEDLVAKGEIVPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITDVFKEDIGIGGVLGLLWFQRRMPKYASHFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGLIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQNFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDLLRTCGTFTREEADEYVDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	XM_012971671.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971671.3

ORF Insert Sequence:

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGA TTCTACAGCC 
TTGCACGGAC CGGCTACAGC CAAAGCCACC ACTCTGTTCA ACCGTCACAC CAAATCCATC 
GTTTGGGGCA TGCAGACCCG AGCCGTGCAG GGCATGCTGG ACTTCGACTA CATCTGCTCC 
CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT TACCCTTTCA CTGGTGATCA CAAGCAAAAG 
TTTTACTGGG GCCACAAAGA GATCCTCATA CCGGTCTTCA AAAACATGGC GGATGCGATG 
AGGAAACACC CCGAGGTGGA CGTGCTCATT AACTTTGCCT CCCTGAGGTC GGCGTATGAC 
AGCACCATCG AGACCATGAA TTACCCGCAG ATCCGTTCAA TTGCAATTAT TGCAGAAGGA 
ATACCAGAAG CACTTACAAG GAAGCTCATC AAGATGGCGG ATGAGAAAGG AGTCACAATT 
ATAGGTCCTG CCACGGTTGG TGGAATTAAA CCTGGTTGCT TTAAGATTGG AAACACAGGG 
GGAATGCTGG ACAATATTCT GGCCTCTAAG CTCTACCGCC CTGGGAGCGT CGCCTATGTG 
TCCCGCTCAG GGGGAATGTC CAACGAGCTC AACAACATCA TCTCCAGGAC CACGGATGGA 
GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC AGATACCCCG GGTCAACATT CATGGACCAT 
GTTCTACGTT ACCAGGACAC TCCAGGAGTT AAAATGATTG TTGTTCTTGG AGAGATTGGG 
GGCACGGAGG AGTACAAGAT CTGCCGTGGT ATTAAGGAAG GCCGGCTGAC TAAACCAATC 
GTCTGTTGGT GTATTGGGAC CTGCGCTACT ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT 
GCAGGAGCTT GTGCCAACCA AGCATCGGAG ACGGCTGTGG CTAAAAACCA GGCCTTGAAG 
GAAGCTGGAG TATTCGTTCC ACGTAGTTTT GATGAACTGG GGGATGTCAT TCAGTCTGTG 
TATGAGGACC TAGTTGCTAA AGGAGAAATT GTACCAGCTG AGGAAGTGCC ACCTCCCACT 
GTACCCATGG ACTACTCCTG GGCGAGGGAG CTGGGTTTGA TCCGGAAGCC TGCATCTTTC 
ATGACTAGTA TTTGTGACGA GAGGGGCCAG GAGCTGATCT ACGCAGGAAT GCCAATCACT 
GATGTCTTCA AGGAAGATAT TGGCATCGGA GGAGTATTGG GCCTGCTGTG GTTCCAGAGA 
AGGATGCCCA AGTATGCCTC TCACTTTATT GAGATGTGCC TGATGGTGAC GGCAGACCAC 
GGGCCGGCTG TGTCGGGGGC CCATAATACC ATTGTGTGTG CCAGAGCCGG AAAAGACCTG 
ATCTCTAGCC TGACGTCTGG ACTGCTTACA ATTGGCGATC GCTTCGGGGG AGCACTTGAT 
GCCGCTGCCA AGATGTTCAG CAAAGCGTTT GACAGCGGCC TTATTCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG GAAGCTGATC ATGGGCATCG GCCACAGAGT CAAATCTATC 
AACAACCCAG ACATGAGAGT ACAGATCCTG AAGGACTACG TCAAGCAGAA CTTCCCGGCC 
ACTCCTTTAC TGGACTATGC CCTCGAGGTG GAAAAGATCA CAACTTCAAA GAAACCAAAC 
CTCATCCTTA ACGTTGATGG CCTTATCGGT GTGGCGTTTG TAGATCTGCT CAGGACCTGC 
GGCACTTTCA CACGGGAGGA AGCGGATGAA TACGTAGATA TTGGAGCACT GAACGGCATC 
TTTGTTTTGG GAAGAAGCAT GGGTTTTATC GGGCATTACT TGGACCAGAA AAGGCTGAAG 
CAGGGATTGT ACCGGCATCC ATGGGACGAT ATTTCCTACG TTCTGCCTGA GCACATGACT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acly ( XM_012971673.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827127.1 Xenopus tropicalis ATP citrate lyase (acly), transcript variant X3, mRNA.

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CloneID	OXa26919
Clone ID Related Accession (Same CDS sequence)	XM_012971673.3 , XM_012971673.2
Accession Version	XM_012971673.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-citrate synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGC CAAAGCCACC 
ACTCTGTTCA ACCGTCACAC CAAATCCATC GTTTGGGGCA TGCAGACCCG AGCCGTGCAG 
GGCATGCTGG ACTTCGACTA CATCTGCTCC CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT 
TACCCTTTCA CTGGTGATCA CAAGCAAAAG TTTTACTGGG GCCACAAAGA GATCCTCATA 
CCGGTCTTCA AAAACATGGC GGATGCGATG AGGAAACACC CCGAGGTGGA CGTGCTCATT 
AACTTTGCCT CCCTGAGGTC GGCGTATGAC AGCACCATCG AGACCATGAA TTACCCGCAG 
ATCCGTTCAA TTGCAATTAT TGCAGAAGGA ATACCAGAAG CACTTACAAG GAAGCTCATC 
AAGATGGCGG ATGAGAAAGG AGTCACAATT ATAGGTCCTG CCACGGTTGG TGGAATTAAA 
CCTGGTTGCT TTAAGATTGG AAACACAGGG GGAATGCTGG ACAATATTCT GGCCTCTAAG 
CTCTACCGCC CTGGGAGCGT CGCCTATGTG TCCCGCTCAG GGGGAATGTC CAACGAGCTC 
AACAACATCA TCTCCAGGAC CACGGATGGA GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC 
AGATACCCCG GGTCAACATT CATGGACCAT GTTCTACGTT ACCAGGACAC TCCAGGAGTT 
AAAATGATTG TTGTTCTTGG AGAGATTGGG GGCACGGAGG AGTACAAGAT CTGCCGTGGT 
ATTAAGGAAG GCCGGCTGAC TAAACCAATC GTCTGTTGGT GTATTGGGAC CTGCGCTACT 
ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT GCAGGAGCTT GTGCCAACCA AGCATCGGAG 
ACGGCTGTGG CTAAAAACCA GGCCTTGAAG GAAGCTGGAG TATTCGTTCC ACGTAGTTTT 
GATGAACTGG GGGATGTCAT TCAGTCTGTG TATGAGGACC TAGTTGCTAA AGGAGAAATT 
GTACCAGCTG AGGAAGTGCC ACCTCCCACT GTACCCATGG ACTACTCCTG GGCGAGGGAG 
CTGGGTTTGA TCCGGAAGCC TGCATCTTTC ATGACTAGTA TTTGTGACGA GAGGGGCCAG 
GAGCTGATCT ACGCAGGAAT GCCAATCACT GATGTCTTCA AGGAAGATAT TGGCATCGGA 
GGAGTATTGG GCCTGCTGTG GTTCCAGAGA AGGATGCCCA AGTATGCCTC TCACTTTATT 
GAGATGTGCC TGATGGTGAC GGCAGACCAC GGGCCGGCTG TGTCGGGGGC CCATAATACC 
ATTGTGTGTG CCAGAGCCGG AAAAGACCTG ATCTCTAGCC TGACGTCTGG ACTGCTTACA 
ATTGGCGATC GCTTCGGGGG AGCACTTGAT GCCGCTGCCA AGATGTTCAG CAAAGCGTTT 
GACAGCGGCC TTATTCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAAGG GAAGCTGATC 
ATGGGCATCG GCCACAGAGT CAAATCTATC AACAACCCAG ACATGAGAGT ACAGATCCTG 
AAGGACTACG TCAAGCAGAA CTTCCCGGCC ACTCCTTTAC TGGACTATGC CCTCGAGGTG 
GAAAAGATCA CAACTTCAAA GAAACCAAAC CTCATCCTTA ACGTTGATGG CCTTATCGGT 
GTGGCGTTTG TAGATCTGCT CAGGACCTGC GGCACTTTCA CACGGGAGGA AGCGGATGAA 
TACGTAGATA TTGGAGCACT GAACGGCATC TTTGTTTTGG GAAGAAGCAT GGGTTTTATC 
GGGCATTACT TGGACCAGAA AAGGCTGAAG CAGGGATTGT ACCGGCATCC ATGGGACGAT 
ATTTCCTACG TTCTGCCTGA GCACATGACT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827127.1
CDS	159..3434
Translation	MSAKAISEQTGKEFLYKYICTTAAVQNRFKYAHVTYDTDWDRLAQEHPWLLTQPLVVKPDQLIKRRGKLGLVGVNLNLDQVKSWLKPRLGNETTIAKAKGILKNFLIEPFVPHKQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDSKAQKLLVGVGERLTEKEVKSRLLIHVAQDKQEVLASFITGLFNLYEDLYFTYLEINPLVVTKDGVYVLDMAAKIDATADYICKAKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPRGRIWTMVAGGGASVVYSDTICDLGGVDELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIKDFQCPLKEHEVMIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSSSTPAPSRTASFSESRTEDITPAKKSKPAIPNAKATTLFNRHTKSIVWGMQTRAVQGMLDFDYICSRSEPSVAALVYPFTGDHKQKFYWGHKEILIPVFKNMADAMRKHPEVDVLINFASLRSAYDSTIETMNYPQIRSIAIIAEGIPEALTRKLIKMADEKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVFEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGDVIQSVYEDLVAKGEIVPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITDVFKEDIGIGGVLGLLWFQRRMPKYASHFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGLIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQNFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDLLRTCGTFTREEADEYVDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	XM_012971673.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971673.3

ORF Insert Sequence:

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGC CAAAGCCACC 
ACTCTGTTCA ACCGTCACAC CAAATCCATC GTTTGGGGCA TGCAGACCCG AGCCGTGCAG 
GGCATGCTGG ACTTCGACTA CATCTGCTCC CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT 
TACCCTTTCA CTGGTGATCA CAAGCAAAAG TTTTACTGGG GCCACAAAGA GATCCTCATA 
CCGGTCTTCA AAAACATGGC GGATGCGATG AGGAAACACC CCGAGGTGGA CGTGCTCATT 
AACTTTGCCT CCCTGAGGTC GGCGTATGAC AGCACCATCG AGACCATGAA TTACCCGCAG 
ATCCGTTCAA TTGCAATTAT TGCAGAAGGA ATACCAGAAG CACTTACAAG GAAGCTCATC 
AAGATGGCGG ATGAGAAAGG AGTCACAATT ATAGGTCCTG CCACGGTTGG TGGAATTAAA 
CCTGGTTGCT TTAAGATTGG AAACACAGGG GGAATGCTGG ACAATATTCT GGCCTCTAAG 
CTCTACCGCC CTGGGAGCGT CGCCTATGTG TCCCGCTCAG GGGGAATGTC CAACGAGCTC 
AACAACATCA TCTCCAGGAC CACGGATGGA GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC 
AGATACCCCG GGTCAACATT CATGGACCAT GTTCTACGTT ACCAGGACAC TCCAGGAGTT 
AAAATGATTG TTGTTCTTGG AGAGATTGGG GGCACGGAGG AGTACAAGAT CTGCCGTGGT 
ATTAAGGAAG GCCGGCTGAC TAAACCAATC GTCTGTTGGT GTATTGGGAC CTGCGCTACT 
ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT GCAGGAGCTT GTGCCAACCA AGCATCGGAG 
ACGGCTGTGG CTAAAAACCA GGCCTTGAAG GAAGCTGGAG TATTCGTTCC ACGTAGTTTT 
GATGAACTGG GGGATGTCAT TCAGTCTGTG TATGAGGACC TAGTTGCTAA AGGAGAAATT 
GTACCAGCTG AGGAAGTGCC ACCTCCCACT GTACCCATGG ACTACTCCTG GGCGAGGGAG 
CTGGGTTTGA TCCGGAAGCC TGCATCTTTC ATGACTAGTA TTTGTGACGA GAGGGGCCAG 
GAGCTGATCT ACGCAGGAAT GCCAATCACT GATGTCTTCA AGGAAGATAT TGGCATCGGA 
GGAGTATTGG GCCTGCTGTG GTTCCAGAGA AGGATGCCCA AGTATGCCTC TCACTTTATT 
GAGATGTGCC TGATGGTGAC GGCAGACCAC GGGCCGGCTG TGTCGGGGGC CCATAATACC 
ATTGTGTGTG CCAGAGCCGG AAAAGACCTG ATCTCTAGCC TGACGTCTGG ACTGCTTACA 
ATTGGCGATC GCTTCGGGGG AGCACTTGAT GCCGCTGCCA AGATGTTCAG CAAAGCGTTT 
GACAGCGGCC TTATTCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAAGG GAAGCTGATC 
ATGGGCATCG GCCACAGAGT CAAATCTATC AACAACCCAG ACATGAGAGT ACAGATCCTG 
AAGGACTACG TCAAGCAGAA CTTCCCGGCC ACTCCTTTAC TGGACTATGC CCTCGAGGTG 
GAAAAGATCA CAACTTCAAA GAAACCAAAC CTCATCCTTA ACGTTGATGG CCTTATCGGT 
GTGGCGTTTG TAGATCTGCT CAGGACCTGC GGCACTTTCA CACGGGAGGA AGCGGATGAA 
TACGTAGATA TTGGAGCACT GAACGGCATC TTTGTTTTGG GAAGAAGCAT GGGTTTTATC 
GGGCATTACT TGGACCAGAA AAGGCTGAAG CAGGGATTGT ACCGGCATCC ATGGGACGAT 
ATTTCCTACG TTCTGCCTGA GCACATGACT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acly ( NM_001008027.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001008028.1 Xenopus tropicalis ATP citrate lyase (acly), mRNA.

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CloneID	OXa01580
Clone ID Related Accession (Same CDS sequence)	NM_001008027.1
Accession Version	NM_001008027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-citrate synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC080908.1. ##Evidence-Data-START## Transcript exon combination :: BC080908.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGAG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGC CAAAGCCACC 
ACTCTGTTCA ACCGTCACAC CAAATCCATC GTTTGGGGCA TGCAGACCCG AGCCGTGCAG 
GGCATGCTGG ACTTCGACTA CATCTGCTCC CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT 
TACCCTTTCA CTGGTGATCA CAAGCAAAAG TTTTACTGGG GCCACAAAGA GATCCTCATA 
CCGGTCTTCA AAAACATGGC GGATGCGATG AGGAAACACC CCGAGGTGGA CGTGCTCATT 
AACTTTGCCT CCCTGAGGTC GGCGTATGAC AGCACCATCG AGACCATGAA TTACCCGCAG 
ATCCGTTCAA TTGCAATTAT TGCAGAAGGA ATACCAGAAG CACTTACAAG GAAGCTCATC 
AAGATGGCGG ATGAGAAAGG AGTCACAATT ATAGGTCCTG CCACGGTTGG TGGAATTAAA 
CCTGGTTGCT TTAAGATTGG AAACACAGGG GGAATGCTGG ACAATATTCT GGCCTCTAAG 
CTCTACCGCC CTGGGAGCGT CGCCTATGTG TCCCGCTCAG GGGGAATGTC CAACGAGCTC 
AACAACATCA TCTCCAGGAC CACGGATGGA GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC 
AGATACCCCG GGTCAACATT CATGGACCAT GTTCTACGTT ACCAGGACAC TCCAGGAGTT 
AAAATGATTG TTGTTCTTGG AGAGATTGGA GGCACGGAGG AGTACAAGAT CTGCCGTGGT 
ATTAAGGAAG GCCGGCTGAC TAAACCAATC GTCTGTTGGT GTATTGGGAC CTGCGCTACT 
ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT GCAGGAGCTT GTGCCAACCA AGCATCGGAG 
ACGGCTGTGG CTAAAAACCA GGCCTTGAAG GAAGCTGGAG TATTCGTTCC ACGTAGTTTT 
GATGAACTGG GGGATGTCAT TCAGTCTGTG TATGAGGACC TAGTTGCTAA AGGAGAAATT 
GTACCAGCTG AGGAAGTGCC ACCTCCCACT GTACCCATGG ACTACTCCTG GGCGAGGGAG 
CTGGGTTTGA TCCGGAAGCC TGCATCTTTC ATGACTAGTA TTTGTGACGA GAGGGGCCAG 
GAGCTGATCT ACGCAGGAAT GCCAATCACT GATGTCTTCA AGGAAGATAT TGGCATCGGA 
GGAGTATTGG GCCTGCTGTG GTTCCAGAGA AGGATGCCCA AGTATGCCTC TCACTTTATT 
GAGATGTGCC TGATGGTGAC GGCAGACCAC GGGCCGGCTG TGTCGGGGGC CCATAATACC 
ATTGTGTGTG CCAGAGCCGG AAAAGACCTG ATCTCTAGCC TGACGTCTGG ACTGCTTACA 
ATTGGCGATC GCTTCGGGGG AGCACTTGAT GCCGCTGCCA AGATGTTCAG CAAAGCGTTT 
GACAGCGGCC TTATTCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAAGG GAAGCTGATC 
ATGGGCATCG GCCACAGAGT CAAATCTATC AACAACCCAG ACATGAGAGT ACAGATCCTG 
AAGGACTACG TCAAGCAGAA CTTCCCGGCC ACTCCTTTAC TGGACTATGC CCTCGAGGTG 
GAAAAGATCA CAACTTCAAA GAAACCAAAC CTCATCCTTA ACGTTGATGG CCTTATCGGT 
GTGGCGTTTG TAGATCTGCT CAGGACCTGC GGCACTTTCA CACGGGAGGA AGCGGATGAA 
TACGTAGATA TTGGAGCACT GAACGGCATC TTTGTTTTGG GAAGAAGCAT GGGATTTATC 
GGGCATTACT TGGACCAGAA AAGGCTGAAG CAGGGATTGT ACCGGCATCC ATGGGACGAT 
ATTTCCTACG TTCTGCCTGA GCACATGACT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008028.1
CDS	71..3346
Translation	MSAKAISEQTGKEFLYKYICTTAAVQNRFKYAHVTYDTDWDRLAQEHPWLLTQPLVVKPDQLIKRRGKLGLVGVNLNLDQVKSWLKPRLGNETTIAKAKGILKNFLIEPFVPHKQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDSKAQKLLVGVGERLTEKEVKSRLLIHVAQDKQEVLASFITGLFNLYEDLYFTYLEINPLVVTKDGVYVLDMAAKIDATADYICKAKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPRGRIWTMVAGGGASVVYSDTICDLGGVDELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIKDFQCPLKEHEVMIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSSSTPAPSRTASFSESRTEDITPAKKSKPAIPNAKATTLFNRHTKSIVWGMQTRAVQGMLDFDYICSRSEPSVAALVYPFTGDHKQKFYWGHKEILIPVFKNMADAMRKHPEVDVLINFASLRSAYDSTIETMNYPQIRSIAIIAEGIPEALTRKLIKMADEKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVFEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGDVIQSVYEDLVAKGEIVPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITDVFKEDIGIGGVLGLLWFQRRMPKYASHFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGLIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQNFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDLLRTCGTFTREEADEYVDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	NM_001008027.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATP citrate lyase (acly), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008027.1

ORF Insert Sequence:

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGAG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGC CAAAGCCACC 
ACTCTGTTCA ACCGTCACAC CAAATCCATC GTTTGGGGCA TGCAGACCCG AGCCGTGCAG 
GGCATGCTGG ACTTCGACTA CATCTGCTCC CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT 
TACCCTTTCA CTGGTGATCA CAAGCAAAAG TTTTACTGGG GCCACAAAGA GATCCTCATA 
CCGGTCTTCA AAAACATGGC GGATGCGATG AGGAAACACC CCGAGGTGGA CGTGCTCATT 
AACTTTGCCT CCCTGAGGTC GGCGTATGAC AGCACCATCG AGACCATGAA TTACCCGCAG 
ATCCGTTCAA TTGCAATTAT TGCAGAAGGA ATACCAGAAG CACTTACAAG GAAGCTCATC 
AAGATGGCGG ATGAGAAAGG AGTCACAATT ATAGGTCCTG CCACGGTTGG TGGAATTAAA 
CCTGGTTGCT TTAAGATTGG AAACACAGGG GGAATGCTGG ACAATATTCT GGCCTCTAAG 
CTCTACCGCC CTGGGAGCGT CGCCTATGTG TCCCGCTCAG GGGGAATGTC CAACGAGCTC 
AACAACATCA TCTCCAGGAC CACGGATGGA GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC 
AGATACCCCG GGTCAACATT CATGGACCAT GTTCTACGTT ACCAGGACAC TCCAGGAGTT 
AAAATGATTG TTGTTCTTGG AGAGATTGGA GGCACGGAGG AGTACAAGAT CTGCCGTGGT 
ATTAAGGAAG GCCGGCTGAC TAAACCAATC GTCTGTTGGT GTATTGGGAC CTGCGCTACT 
ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT GCAGGAGCTT GTGCCAACCA AGCATCGGAG 
ACGGCTGTGG CTAAAAACCA GGCCTTGAAG GAAGCTGGAG TATTCGTTCC ACGTAGTTTT 
GATGAACTGG GGGATGTCAT TCAGTCTGTG TATGAGGACC TAGTTGCTAA AGGAGAAATT 
GTACCAGCTG AGGAAGTGCC ACCTCCCACT GTACCCATGG ACTACTCCTG GGCGAGGGAG 
CTGGGTTTGA TCCGGAAGCC TGCATCTTTC ATGACTAGTA TTTGTGACGA GAGGGGCCAG 
GAGCTGATCT ACGCAGGAAT GCCAATCACT GATGTCTTCA AGGAAGATAT TGGCATCGGA 
GGAGTATTGG GCCTGCTGTG GTTCCAGAGA AGGATGCCCA AGTATGCCTC TCACTTTATT 
GAGATGTGCC TGATGGTGAC GGCAGACCAC GGGCCGGCTG TGTCGGGGGC CCATAATACC 
ATTGTGTGTG CCAGAGCCGG AAAAGACCTG ATCTCTAGCC TGACGTCTGG ACTGCTTACA 
ATTGGCGATC GCTTCGGGGG AGCACTTGAT GCCGCTGCCA AGATGTTCAG CAAAGCGTTT 
GACAGCGGCC TTATTCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAAGG GAAGCTGATC 
ATGGGCATCG GCCACAGAGT CAAATCTATC AACAACCCAG ACATGAGAGT ACAGATCCTG 
AAGGACTACG TCAAGCAGAA CTTCCCGGCC ACTCCTTTAC TGGACTATGC CCTCGAGGTG 
GAAAAGATCA CAACTTCAAA GAAACCAAAC CTCATCCTTA ACGTTGATGG CCTTATCGGT 
GTGGCGTTTG TAGATCTGCT CAGGACCTGC GGCACTTTCA CACGGGAGGA AGCGGATGAA 
TACGTAGATA TTGGAGCACT GAACGGCATC TTTGTTTTGG GAAGAAGCAT GGGATTTATC 
GGGCATTACT TGGACCAGAA AAGGCTGAAG CAGGGATTGT ACCGGCATCC ATGGGACGAT 
ATTTCCTACG TTCTGCCTGA GCACATGACT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acly ( XM_012971672.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827126.1 Xenopus tropicalis ATP citrate lyase (acly), transcript variant X2, mRNA.

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CloneID	OXa26918
Clone ID Related Accession (Same CDS sequence)	XM_012971672.2 , XM_012971672.3 , XM_012971671.2 , XM_012971671.3
Accession Version	XM_012971672.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-citrate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGA TTCTACAGCC 
TTGCACGGAC CGGCTACAGC CAAAGCCACC ACTCTGTTCA ACCGTCACAC CAAATCCATC 
GTTTGGGGCA TGCAGACCCG AGCCGTGCAG GGCATGCTGG ACTTCGACTA CATCTGCTCC 
CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT TACCCTTTCA CTGGTGATCA CAAGCAAAAG 
TTTTACTGGG GCCACAAAGA GATCCTCATA CCGGTCTTCA AAAACATGGC GGATGCGATG 
AGGAAACACC CCGAGGTGGA CGTGCTCATT AACTTTGCCT CCCTGAGGTC GGCGTATGAC 
AGCACCATCG AGACCATGAA TTACCCGCAG ATCCGTTCAA TTGCAATTAT TGCAGAAGGA 
ATACCAGAAG CACTTACAAG GAAGCTCATC AAGATGGCGG ATGAGAAAGG AGTCACAATT 
ATAGGTCCTG CCACGGTTGG TGGAATTAAA CCTGGTTGCT TTAAGATTGG AAACACAGGG 
GGAATGCTGG ACAATATTCT GGCCTCTAAG CTCTACCGCC CTGGGAGCGT CGCCTATGTG 
TCCCGCTCAG GGGGAATGTC CAACGAGCTC AACAACATCA TCTCCAGGAC CACGGATGGA 
GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC AGATACCCCG GGTCAACATT CATGGACCAT 
GTTCTACGTT ACCAGGACAC TCCAGGAGTT AAAATGATTG TTGTTCTTGG AGAGATTGGG 
GGCACGGAGG AGTACAAGAT CTGCCGTGGT ATTAAGGAAG GCCGGCTGAC TAAACCAATC 
GTCTGTTGGT GTATTGGGAC CTGCGCTACT ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT 
GCAGGAGCTT GTGCCAACCA AGCATCGGAG ACGGCTGTGG CTAAAAACCA GGCCTTGAAG 
GAAGCTGGAG TATTCGTTCC ACGTAGTTTT GATGAACTGG GGGATGTCAT TCAGTCTGTG 
TATGAGGACC TAGTTGCTAA AGGAGAAATT GTACCAGCTG AGGAAGTGCC ACCTCCCACT 
GTACCCATGG ACTACTCCTG GGCGAGGGAG CTGGGTTTGA TCCGGAAGCC TGCATCTTTC 
ATGACTAGTA TTTGTGACGA GAGGGGCCAG GAGCTGATCT ACGCAGGAAT GCCAATCACT 
GATGTCTTCA AGGAAGATAT TGGCATCGGA GGAGTATTGG GCCTGCTGTG GTTCCAGAGA 
AGGATGCCCA AGTATGCCTC TCACTTTATT GAGATGTGCC TGATGGTGAC GGCAGACCAC 
GGGCCGGCTG TGTCGGGGGC CCATAATACC ATTGTGTGTG CCAGAGCCGG AAAAGACCTG 
ATCTCTAGCC TGACGTCTGG ACTGCTTACA ATTGGCGATC GCTTCGGGGG AGCACTTGAT 
GCCGCTGCCA AGATGTTCAG CAAAGCGTTT GACAGCGGCC TTATTCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG GAAGCTGATC ATGGGCATCG GCCACAGAGT CAAATCTATC 
AACAACCCAG ACATGAGAGT ACAGATCCTG AAGGACTACG TCAAGCAGAA CTTCCCGGCC 
ACTCCTTTAC TGGACTATGC CCTCGAGGTG GAAAAGATCA CAACTTCAAA GAAACCAAAC 
CTCATCCTTA ACGTTGATGG CCTTATCGGT GTGGCGTTTG TAGATCTGCT CAGGACCTGC 
GGCACTTTCA CACGGGAGGA AGCGGATGAA TACGTAGATA TTGGAGCACT GAACGGCATC 
TTTGTTTTGG GAAGAAGCAT GGGTTTTATC GGGCATTACT TGGACCAGAA AAGGCTGAAG 
CAGGGATTGT ACCGGCATCC ATGGGACGAT ATTTCCTACG TTCTGCCTGA GCACATGACT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827126.1
CDS	154..3459
Translation	MSAKAISEQTGKEFLYKYICTTAAVQNRFKYAHVTYDTDWDRLAQEHPWLLTQPLVVKPDQLIKRRGKLGLVGVNLNLDQVKSWLKPRLGNETTIAKAKGILKNFLIEPFVPHKQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDSKAQKLLVGVGERLTEKEVKSRLLIHVAQDKQEVLASFITGLFNLYEDLYFTYLEINPLVVTKDGVYVLDMAAKIDATADYICKAKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPRGRIWTMVAGGGASVVYSDTICDLGGVDELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIKDFQCPLKEHEVMIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSSSTPAPSRTASFSESRTEDITPAKKSKPAIPNDSTALHGPATAKATTLFNRHTKSIVWGMQTRAVQGMLDFDYICSRSEPSVAALVYPFTGDHKQKFYWGHKEILIPVFKNMADAMRKHPEVDVLINFASLRSAYDSTIETMNYPQIRSIAIIAEGIPEALTRKLIKMADEKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVFEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGDVIQSVYEDLVAKGEIVPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITDVFKEDIGIGGVLGLLWFQRRMPKYASHFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGLIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQNFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDLLRTCGTFTREEADEYVDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	XM_012971672.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971672.2

ORF Insert Sequence:

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGA TTCTACAGCC 
TTGCACGGAC CGGCTACAGC CAAAGCCACC ACTCTGTTCA ACCGTCACAC CAAATCCATC 
GTTTGGGGCA TGCAGACCCG AGCCGTGCAG GGCATGCTGG ACTTCGACTA CATCTGCTCC 
CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT TACCCTTTCA CTGGTGATCA CAAGCAAAAG 
TTTTACTGGG GCCACAAAGA GATCCTCATA CCGGTCTTCA AAAACATGGC GGATGCGATG 
AGGAAACACC CCGAGGTGGA CGTGCTCATT AACTTTGCCT CCCTGAGGTC GGCGTATGAC 
AGCACCATCG AGACCATGAA TTACCCGCAG ATCCGTTCAA TTGCAATTAT TGCAGAAGGA 
ATACCAGAAG CACTTACAAG GAAGCTCATC AAGATGGCGG ATGAGAAAGG AGTCACAATT 
ATAGGTCCTG CCACGGTTGG TGGAATTAAA CCTGGTTGCT TTAAGATTGG AAACACAGGG 
GGAATGCTGG ACAATATTCT GGCCTCTAAG CTCTACCGCC CTGGGAGCGT CGCCTATGTG 
TCCCGCTCAG GGGGAATGTC CAACGAGCTC AACAACATCA TCTCCAGGAC CACGGATGGA 
GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC AGATACCCCG GGTCAACATT CATGGACCAT 
GTTCTACGTT ACCAGGACAC TCCAGGAGTT AAAATGATTG TTGTTCTTGG AGAGATTGGG 
GGCACGGAGG AGTACAAGAT CTGCCGTGGT ATTAAGGAAG GCCGGCTGAC TAAACCAATC 
GTCTGTTGGT GTATTGGGAC CTGCGCTACT ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT 
GCAGGAGCTT GTGCCAACCA AGCATCGGAG ACGGCTGTGG CTAAAAACCA GGCCTTGAAG 
GAAGCTGGAG TATTCGTTCC ACGTAGTTTT GATGAACTGG GGGATGTCAT TCAGTCTGTG 
TATGAGGACC TAGTTGCTAA AGGAGAAATT GTACCAGCTG AGGAAGTGCC ACCTCCCACT 
GTACCCATGG ACTACTCCTG GGCGAGGGAG CTGGGTTTGA TCCGGAAGCC TGCATCTTTC 
ATGACTAGTA TTTGTGACGA GAGGGGCCAG GAGCTGATCT ACGCAGGAAT GCCAATCACT 
GATGTCTTCA AGGAAGATAT TGGCATCGGA GGAGTATTGG GCCTGCTGTG GTTCCAGAGA 
AGGATGCCCA AGTATGCCTC TCACTTTATT GAGATGTGCC TGATGGTGAC GGCAGACCAC 
GGGCCGGCTG TGTCGGGGGC CCATAATACC ATTGTGTGTG CCAGAGCCGG AAAAGACCTG 
ATCTCTAGCC TGACGTCTGG ACTGCTTACA ATTGGCGATC GCTTCGGGGG AGCACTTGAT 
GCCGCTGCCA AGATGTTCAG CAAAGCGTTT GACAGCGGCC TTATTCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG GAAGCTGATC ATGGGCATCG GCCACAGAGT CAAATCTATC 
AACAACCCAG ACATGAGAGT ACAGATCCTG AAGGACTACG TCAAGCAGAA CTTCCCGGCC 
ACTCCTTTAC TGGACTATGC CCTCGAGGTG GAAAAGATCA CAACTTCAAA GAAACCAAAC 
CTCATCCTTA ACGTTGATGG CCTTATCGGT GTGGCGTTTG TAGATCTGCT CAGGACCTGC 
GGCACTTTCA CACGGGAGGA AGCGGATGAA TACGTAGATA TTGGAGCACT GAACGGCATC 
TTTGTTTTGG GAAGAAGCAT GGGTTTTATC GGGCATTACT TGGACCAGAA AAGGCTGAAG 
CAGGGATTGT ACCGGCATCC ATGGGACGAT ATTTCCTACG TTCTGCCTGA GCACATGACT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acly ( XM_012971671.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827125.1 Xenopus tropicalis ATP citrate lyase (acly), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa26918
Clone ID Related Accession (Same CDS sequence)	XM_012971672.2 , XM_012971672.3 , XM_012971671.2 , XM_012971671.3
Accession Version	XM_012971671.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-citrate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGA TTCTACAGCC 
TTGCACGGAC CGGCTACAGC CAAAGCCACC ACTCTGTTCA ACCGTCACAC CAAATCCATC 
GTTTGGGGCA TGCAGACCCG AGCCGTGCAG GGCATGCTGG ACTTCGACTA CATCTGCTCC 
CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT TACCCTTTCA CTGGTGATCA CAAGCAAAAG 
TTTTACTGGG GCCACAAAGA GATCCTCATA CCGGTCTTCA AAAACATGGC GGATGCGATG 
AGGAAACACC CCGAGGTGGA CGTGCTCATT AACTTTGCCT CCCTGAGGTC GGCGTATGAC 
AGCACCATCG AGACCATGAA TTACCCGCAG ATCCGTTCAA TTGCAATTAT TGCAGAAGGA 
ATACCAGAAG CACTTACAAG GAAGCTCATC AAGATGGCGG ATGAGAAAGG AGTCACAATT 
ATAGGTCCTG CCACGGTTGG TGGAATTAAA CCTGGTTGCT TTAAGATTGG AAACACAGGG 
GGAATGCTGG ACAATATTCT GGCCTCTAAG CTCTACCGCC CTGGGAGCGT CGCCTATGTG 
TCCCGCTCAG GGGGAATGTC CAACGAGCTC AACAACATCA TCTCCAGGAC CACGGATGGA 
GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC AGATACCCCG GGTCAACATT CATGGACCAT 
GTTCTACGTT ACCAGGACAC TCCAGGAGTT AAAATGATTG TTGTTCTTGG AGAGATTGGG 
GGCACGGAGG AGTACAAGAT CTGCCGTGGT ATTAAGGAAG GCCGGCTGAC TAAACCAATC 
GTCTGTTGGT GTATTGGGAC CTGCGCTACT ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT 
GCAGGAGCTT GTGCCAACCA AGCATCGGAG ACGGCTGTGG CTAAAAACCA GGCCTTGAAG 
GAAGCTGGAG TATTCGTTCC ACGTAGTTTT GATGAACTGG GGGATGTCAT TCAGTCTGTG 
TATGAGGACC TAGTTGCTAA AGGAGAAATT GTACCAGCTG AGGAAGTGCC ACCTCCCACT 
GTACCCATGG ACTACTCCTG GGCGAGGGAG CTGGGTTTGA TCCGGAAGCC TGCATCTTTC 
ATGACTAGTA TTTGTGACGA GAGGGGCCAG GAGCTGATCT ACGCAGGAAT GCCAATCACT 
GATGTCTTCA AGGAAGATAT TGGCATCGGA GGAGTATTGG GCCTGCTGTG GTTCCAGAGA 
AGGATGCCCA AGTATGCCTC TCACTTTATT GAGATGTGCC TGATGGTGAC GGCAGACCAC 
GGGCCGGCTG TGTCGGGGGC CCATAATACC ATTGTGTGTG CCAGAGCCGG AAAAGACCTG 
ATCTCTAGCC TGACGTCTGG ACTGCTTACA ATTGGCGATC GCTTCGGGGG AGCACTTGAT 
GCCGCTGCCA AGATGTTCAG CAAAGCGTTT GACAGCGGCC TTATTCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG GAAGCTGATC ATGGGCATCG GCCACAGAGT CAAATCTATC 
AACAACCCAG ACATGAGAGT ACAGATCCTG AAGGACTACG TCAAGCAGAA CTTCCCGGCC 
ACTCCTTTAC TGGACTATGC CCTCGAGGTG GAAAAGATCA CAACTTCAAA GAAACCAAAC 
CTCATCCTTA ACGTTGATGG CCTTATCGGT GTGGCGTTTG TAGATCTGCT CAGGACCTGC 
GGCACTTTCA CACGGGAGGA AGCGGATGAA TACGTAGATA TTGGAGCACT GAACGGCATC 
TTTGTTTTGG GAAGAAGCAT GGGTTTTATC GGGCATTACT TGGACCAGAA AAGGCTGAAG 
CAGGGATTGT ACCGGCATCC ATGGGACGAT ATTTCCTACG TTCTGCCTGA GCACATGACT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827125.1
CDS	166..3471
Translation	MSAKAISEQTGKEFLYKYICTTAAVQNRFKYAHVTYDTDWDRLAQEHPWLLTQPLVVKPDQLIKRRGKLGLVGVNLNLDQVKSWLKPRLGNETTIAKAKGILKNFLIEPFVPHKQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDSKAQKLLVGVGERLTEKEVKSRLLIHVAQDKQEVLASFITGLFNLYEDLYFTYLEINPLVVTKDGVYVLDMAAKIDATADYICKAKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPRGRIWTMVAGGGASVVYSDTICDLGGVDELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIKDFQCPLKEHEVMIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSSSTPAPSRTASFSESRTEDITPAKKSKPAIPNDSTALHGPATAKATTLFNRHTKSIVWGMQTRAVQGMLDFDYICSRSEPSVAALVYPFTGDHKQKFYWGHKEILIPVFKNMADAMRKHPEVDVLINFASLRSAYDSTIETMNYPQIRSIAIIAEGIPEALTRKLIKMADEKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVFEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGDVIQSVYEDLVAKGEIVPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITDVFKEDIGIGGVLGLLWFQRRMPKYASHFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGLIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQNFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDLLRTCGTFTREEADEYVDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	XM_012971671.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971671.2

ORF Insert Sequence:

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGA TTCTACAGCC 
TTGCACGGAC CGGCTACAGC CAAAGCCACC ACTCTGTTCA ACCGTCACAC CAAATCCATC 
GTTTGGGGCA TGCAGACCCG AGCCGTGCAG GGCATGCTGG ACTTCGACTA CATCTGCTCC 
CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT TACCCTTTCA CTGGTGATCA CAAGCAAAAG 
TTTTACTGGG GCCACAAAGA GATCCTCATA CCGGTCTTCA AAAACATGGC GGATGCGATG 
AGGAAACACC CCGAGGTGGA CGTGCTCATT AACTTTGCCT CCCTGAGGTC GGCGTATGAC 
AGCACCATCG AGACCATGAA TTACCCGCAG ATCCGTTCAA TTGCAATTAT TGCAGAAGGA 
ATACCAGAAG CACTTACAAG GAAGCTCATC AAGATGGCGG ATGAGAAAGG AGTCACAATT 
ATAGGTCCTG CCACGGTTGG TGGAATTAAA CCTGGTTGCT TTAAGATTGG AAACACAGGG 
GGAATGCTGG ACAATATTCT GGCCTCTAAG CTCTACCGCC CTGGGAGCGT CGCCTATGTG 
TCCCGCTCAG GGGGAATGTC CAACGAGCTC AACAACATCA TCTCCAGGAC CACGGATGGA 
GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC AGATACCCCG GGTCAACATT CATGGACCAT 
GTTCTACGTT ACCAGGACAC TCCAGGAGTT AAAATGATTG TTGTTCTTGG AGAGATTGGG 
GGCACGGAGG AGTACAAGAT CTGCCGTGGT ATTAAGGAAG GCCGGCTGAC TAAACCAATC 
GTCTGTTGGT GTATTGGGAC CTGCGCTACT ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT 
GCAGGAGCTT GTGCCAACCA AGCATCGGAG ACGGCTGTGG CTAAAAACCA GGCCTTGAAG 
GAAGCTGGAG TATTCGTTCC ACGTAGTTTT GATGAACTGG GGGATGTCAT TCAGTCTGTG 
TATGAGGACC TAGTTGCTAA AGGAGAAATT GTACCAGCTG AGGAAGTGCC ACCTCCCACT 
GTACCCATGG ACTACTCCTG GGCGAGGGAG CTGGGTTTGA TCCGGAAGCC TGCATCTTTC 
ATGACTAGTA TTTGTGACGA GAGGGGCCAG GAGCTGATCT ACGCAGGAAT GCCAATCACT 
GATGTCTTCA AGGAAGATAT TGGCATCGGA GGAGTATTGG GCCTGCTGTG GTTCCAGAGA 
AGGATGCCCA AGTATGCCTC TCACTTTATT GAGATGTGCC TGATGGTGAC GGCAGACCAC 
GGGCCGGCTG TGTCGGGGGC CCATAATACC ATTGTGTGTG CCAGAGCCGG AAAAGACCTG 
ATCTCTAGCC TGACGTCTGG ACTGCTTACA ATTGGCGATC GCTTCGGGGG AGCACTTGAT 
GCCGCTGCCA AGATGTTCAG CAAAGCGTTT GACAGCGGCC TTATTCCCAT GGAGTTTGTG 
AACAAGATGA AGAAGGAAGG GAAGCTGATC ATGGGCATCG GCCACAGAGT CAAATCTATC 
AACAACCCAG ACATGAGAGT ACAGATCCTG AAGGACTACG TCAAGCAGAA CTTCCCGGCC 
ACTCCTTTAC TGGACTATGC CCTCGAGGTG GAAAAGATCA CAACTTCAAA GAAACCAAAC 
CTCATCCTTA ACGTTGATGG CCTTATCGGT GTGGCGTTTG TAGATCTGCT CAGGACCTGC 
GGCACTTTCA CACGGGAGGA AGCGGATGAA TACGTAGATA TTGGAGCACT GAACGGCATC 
TTTGTTTTGG GAAGAAGCAT GGGTTTTATC GGGCATTACT TGGACCAGAA AAGGCTGAAG 
CAGGGATTGT ACCGGCATCC ATGGGACGAT ATTTCCTACG TTCTGCCTGA GCACATGACT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acly ( XM_012971673.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827127.1 Xenopus tropicalis ATP citrate lyase (acly), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa26919
Clone ID Related Accession (Same CDS sequence)	XM_012971673.3 , XM_012971673.2
Accession Version	XM_012971673.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-citrate synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGC CAAAGCCACC 
ACTCTGTTCA ACCGTCACAC CAAATCCATC GTTTGGGGCA TGCAGACCCG AGCCGTGCAG 
GGCATGCTGG ACTTCGACTA CATCTGCTCC CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT 
TACCCTTTCA CTGGTGATCA CAAGCAAAAG TTTTACTGGG GCCACAAAGA GATCCTCATA 
CCGGTCTTCA AAAACATGGC GGATGCGATG AGGAAACACC CCGAGGTGGA CGTGCTCATT 
AACTTTGCCT CCCTGAGGTC GGCGTATGAC AGCACCATCG AGACCATGAA TTACCCGCAG 
ATCCGTTCAA TTGCAATTAT TGCAGAAGGA ATACCAGAAG CACTTACAAG GAAGCTCATC 
AAGATGGCGG ATGAGAAAGG AGTCACAATT ATAGGTCCTG CCACGGTTGG TGGAATTAAA 
CCTGGTTGCT TTAAGATTGG AAACACAGGG GGAATGCTGG ACAATATTCT GGCCTCTAAG 
CTCTACCGCC CTGGGAGCGT CGCCTATGTG TCCCGCTCAG GGGGAATGTC CAACGAGCTC 
AACAACATCA TCTCCAGGAC CACGGATGGA GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC 
AGATACCCCG GGTCAACATT CATGGACCAT GTTCTACGTT ACCAGGACAC TCCAGGAGTT 
AAAATGATTG TTGTTCTTGG AGAGATTGGG GGCACGGAGG AGTACAAGAT CTGCCGTGGT 
ATTAAGGAAG GCCGGCTGAC TAAACCAATC GTCTGTTGGT GTATTGGGAC CTGCGCTACT 
ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT GCAGGAGCTT GTGCCAACCA AGCATCGGAG 
ACGGCTGTGG CTAAAAACCA GGCCTTGAAG GAAGCTGGAG TATTCGTTCC ACGTAGTTTT 
GATGAACTGG GGGATGTCAT TCAGTCTGTG TATGAGGACC TAGTTGCTAA AGGAGAAATT 
GTACCAGCTG AGGAAGTGCC ACCTCCCACT GTACCCATGG ACTACTCCTG GGCGAGGGAG 
CTGGGTTTGA TCCGGAAGCC TGCATCTTTC ATGACTAGTA TTTGTGACGA GAGGGGCCAG 
GAGCTGATCT ACGCAGGAAT GCCAATCACT GATGTCTTCA AGGAAGATAT TGGCATCGGA 
GGAGTATTGG GCCTGCTGTG GTTCCAGAGA AGGATGCCCA AGTATGCCTC TCACTTTATT 
GAGATGTGCC TGATGGTGAC GGCAGACCAC GGGCCGGCTG TGTCGGGGGC CCATAATACC 
ATTGTGTGTG CCAGAGCCGG AAAAGACCTG ATCTCTAGCC TGACGTCTGG ACTGCTTACA 
ATTGGCGATC GCTTCGGGGG AGCACTTGAT GCCGCTGCCA AGATGTTCAG CAAAGCGTTT 
GACAGCGGCC TTATTCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAAGG GAAGCTGATC 
ATGGGCATCG GCCACAGAGT CAAATCTATC AACAACCCAG ACATGAGAGT ACAGATCCTG 
AAGGACTACG TCAAGCAGAA CTTCCCGGCC ACTCCTTTAC TGGACTATGC CCTCGAGGTG 
GAAAAGATCA CAACTTCAAA GAAACCAAAC CTCATCCTTA ACGTTGATGG CCTTATCGGT 
GTGGCGTTTG TAGATCTGCT CAGGACCTGC GGCACTTTCA CACGGGAGGA AGCGGATGAA 
TACGTAGATA TTGGAGCACT GAACGGCATC TTTGTTTTGG GAAGAAGCAT GGGTTTTATC 
GGGCATTACT TGGACCAGAA AAGGCTGAAG CAGGGATTGT ACCGGCATCC ATGGGACGAT 
ATTTCCTACG TTCTGCCTGA GCACATGACT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827127.1
CDS	166..3441
Translation	MSAKAISEQTGKEFLYKYICTTAAVQNRFKYAHVTYDTDWDRLAQEHPWLLTQPLVVKPDQLIKRRGKLGLVGVNLNLDQVKSWLKPRLGNETTIAKAKGILKNFLIEPFVPHKQEEEFYVCIYAAREGDYVLFHHEGGVDVGDVDSKAQKLLVGVGERLTEKEVKSRLLIHVAQDKQEVLASFITGLFNLYEDLYFTYLEINPLVVTKDGVYVLDMAAKIDATADYICKAKWGDVEFPPPFGREAYPEEAYIADLDAKSGASLKLTILNPRGRIWTMVAGGGASVVYSDTICDLGGVDELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIKDFQCPLKEHEVMIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSSSTPAPSRTASFSESRTEDITPAKKSKPAIPNAKATTLFNRHTKSIVWGMQTRAVQGMLDFDYICSRSEPSVAALVYPFTGDHKQKFYWGHKEILIPVFKNMADAMRKHPEVDVLINFASLRSAYDSTIETMNYPQIRSIAIIAEGIPEALTRKLIKMADEKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVFEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGDVIQSVYEDLVAKGEIVPAEEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITDVFKEDIGIGGVLGLLWFQRRMPKYASHFIEMCLMVTADHGPAVSGAHNTIVCARAGKDLISSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGLIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVKQNFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDLLRTCGTFTREEADEYVDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMTM

Target ORF information:

RefSeq Version	XM_012971673.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATP citrate lyase (acly), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971673.2

ORF Insert Sequence:

ATGTCAGCCA AGGCGATATC CGAACAGACC GGAAAGGAGT TCCTATACAA GTACATCTGT 
ACCACGGCAG CCGTGCAGAA CCGCTTCAAA TATGCCCATG TTACCTACGA CACCGACTGG 
GACCGTCTGG CTCAGGAACA CCCGTGGCTT CTTACTCAGC CCCTGGTGGT AAAGCCTGAC 
CAGCTCATCA AGCGTCGTGG GAAACTGGGA CTCGTTGGCG TTAACCTCAA CTTGGACCAA 
GTGAAGAGCT GGTTGAAACC ACGTCTTGGG AATGAAACCA CAATTGCTAA AGCCAAGGGG 
ATACTGAAGA ATTTCCTGAT TGAACCGTTT GTCCCCCATA AGCAGGAGGA GGAGTTCTAT 
GTCTGTATCT ATGCAGCCCG AGAAGGAGAT TATGTGCTGT TCCACCATGA GGGGGGCGTA 
GATGTGGGTG ATGTGGACTC CAAAGCCCAA AAGCTGCTGG TTGGTGTAGG AGAACGTTTG 
ACAGAGAAAG AAGTCAAGTC CCGCCTTCTG ATCCATGTGG CCCAAGACAA ACAAGAGGTC 
CTCGCCAGCT TCATCACTGG CCTCTTTAAC CTGTATGAAG ACCTGTACTT TACCTACCTA 
GAGATAAACC CACTAGTTGT TACAAAGGAT GGAGTGTACG TGTTGGACAT GGCGGCCAAG 
ATCGACGCTA CGGCTGACTA TATATGCAAA GCCAAATGGG GCGACGTGGA ATTCCCACCA 
CCGTTCGGCA GAGAAGCTTA TCCTGAGGAG GCCTACATAG CTGATCTGGA TGCCAAGAGT 
GGTGCCAGCC TGAAACTTAC TATCCTCAAC CCACGGGGTA GGATCTGGAC CATGGTGGCA 
GGGGGAGGAG CTTCTGTCGT GTACAGTGAC ACCATCTGTG ACCTGGGAGG GGTGGACGAA 
CTGGCCAATT ACGGAGAGTA TTCCGGTGCC CCGAGTGAGC AGCAGACCTA TGACTATGCC 
AAGACCATCT TGTCCCTTAT GACGAGAGAG AAACACCCAG ATGGTAAAAT CCTTATCATC 
GGGGGAAGCA TTGCCAATTT TACCAACGTC GCTGCAACCT TCAAGGGCAT TGTCAGAGCC 
ATTAAAGATT TCCAGTGCCC TCTGAAAGAA CACGAAGTGA TGATCTTTGT GAGGAGAGGG 
GGACCGAATT ATCAGGAGGG GCTTCGCGTA ATGGGAGAAG TCGGTAAGAC CACGGGGATT 
CCCATCCATG TGTTTGGCAC AGAGACGCAC ATGACGGCCA TTGTAGGCAT GGCTCTAGGT 
CACCGACCTA TCCCTAACCA GCCTCCCACT GCCGCTCATA CGGCCAACTT TCTGCTGAAT 
GCCAGCGGCA GCTCTTCGAC ACCAGCTCCG AGCCGAACTG CATCTTTCTC AGAATCAAGA 
ACAGAAGACA TTACTCCGGC CAAAAAATCC AAGCCAGCTA TACCTAATGC CAAAGCCACC 
ACTCTGTTCA ACCGTCACAC CAAATCCATC GTTTGGGGCA TGCAGACCCG AGCCGTGCAG 
GGCATGCTGG ACTTCGACTA CATCTGCTCC CGCTCAGAAC CCTCTGTGGC CGCCCTGGTT 
TACCCTTTCA CTGGTGATCA CAAGCAAAAG TTTTACTGGG GCCACAAAGA GATCCTCATA 
CCGGTCTTCA AAAACATGGC GGATGCGATG AGGAAACACC CCGAGGTGGA CGTGCTCATT 
AACTTTGCCT CCCTGAGGTC GGCGTATGAC AGCACCATCG AGACCATGAA TTACCCGCAG 
ATCCGTTCAA TTGCAATTAT TGCAGAAGGA ATACCAGAAG CACTTACAAG GAAGCTCATC 
AAGATGGCGG ATGAGAAAGG AGTCACAATT ATAGGTCCTG CCACGGTTGG TGGAATTAAA 
CCTGGTTGCT TTAAGATTGG AAACACAGGG GGAATGCTGG ACAATATTCT GGCCTCTAAG 
CTCTACCGCC CTGGGAGCGT CGCCTATGTG TCCCGCTCAG GGGGAATGTC CAACGAGCTC 
AACAACATCA TCTCCAGGAC CACGGATGGA GTGTTTGAAG GAGTGGCCAT TGGCGGGGAC 
AGATACCCCG GGTCAACATT CATGGACCAT GTTCTACGTT ACCAGGACAC TCCAGGAGTT 
AAAATGATTG TTGTTCTTGG AGAGATTGGG GGCACGGAGG AGTACAAGAT CTGCCGTGGT 
ATTAAGGAAG GCCGGCTGAC TAAACCAATC GTCTGTTGGT GTATTGGGAC CTGCGCTACT 
ATGTTTTCCT CAGAGGTGCA GTTCGGCCAT GCAGGAGCTT GTGCCAACCA AGCATCGGAG 
ACGGCTGTGG CTAAAAACCA GGCCTTGAAG GAAGCTGGAG TATTCGTTCC ACGTAGTTTT 
GATGAACTGG GGGATGTCAT TCAGTCTGTG TATGAGGACC TAGTTGCTAA AGGAGAAATT 
GTACCAGCTG AGGAAGTGCC ACCTCCCACT GTACCCATGG ACTACTCCTG GGCGAGGGAG 
CTGGGTTTGA TCCGGAAGCC TGCATCTTTC ATGACTAGTA TTTGTGACGA GAGGGGCCAG 
GAGCTGATCT ACGCAGGAAT GCCAATCACT GATGTCTTCA AGGAAGATAT TGGCATCGGA 
GGAGTATTGG GCCTGCTGTG GTTCCAGAGA AGGATGCCCA AGTATGCCTC TCACTTTATT 
GAGATGTGCC TGATGGTGAC GGCAGACCAC GGGCCGGCTG TGTCGGGGGC CCATAATACC 
ATTGTGTGTG CCAGAGCCGG AAAAGACCTG ATCTCTAGCC TGACGTCTGG ACTGCTTACA 
ATTGGCGATC GCTTCGGGGG AGCACTTGAT GCCGCTGCCA AGATGTTCAG CAAAGCGTTT 
GACAGCGGCC TTATTCCCAT GGAGTTTGTG AACAAGATGA AGAAGGAAGG GAAGCTGATC 
ATGGGCATCG GCCACAGAGT CAAATCTATC AACAACCCAG ACATGAGAGT ACAGATCCTG 
AAGGACTACG TCAAGCAGAA CTTCCCGGCC ACTCCTTTAC TGGACTATGC CCTCGAGGTG 
GAAAAGATCA CAACTTCAAA GAAACCAAAC CTCATCCTTA ACGTTGATGG CCTTATCGGT 
GTGGCGTTTG TAGATCTGCT CAGGACCTGC GGCACTTTCA CACGGGAGGA AGCGGATGAA 
TACGTAGATA TTGGAGCACT GAACGGCATC TTTGTTTTGG GAAGAAGCAT GGGTTTTATC 
GGGCATTACT TGGACCAGAA AAGGCTGAAG CAGGGATTGT ACCGGCATCC ATGGGACGAT 
ATTTCCTACG TTCTGCCTGA GCACATGACT ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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acly cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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