chc-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	chc-1
Entrez Gene ID	176434
Full Name	Probable clathrin heavy chain 1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_066859.4	NP_499260.1	Probable clathrin heavy chain 1
NM_066859.5	NP_499260.1	Probable clathrin heavy chain 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cltca	NP_001005391.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003021	XP_311856.3
Magnaporthe oryzae (rice blast fungus)	MGG_07768	XP_003712981.1
Homo sapiens (human)	CLTC	NP_001275582.1
Bos taurus (cattle)	CLTC	NP_776448.1
Mus musculus (house mouse)	Cltc	NP_001003908.1
Schizosaccharomyces pombe (fission yeast)	chc1	NP_594148.1
Arabidopsis thaliana (thale cress)	AT3G08530	NP_187466.4
Kluyveromyces lactis	KLLA0F09911g	XP_455531.1
Eremothecium gossypii	AGOS_AER359W	NP_985215.2
Xenopus tropicalis (tropical clawed frog)	cltc	NP_001011039.1
Pan troglodytes (chimpanzee)	CLTC	XP_001136053.1
	CLTC	XP_001108647.2
Gallus gallus (chicken)	CLTC	NP_001073586.1
Danio rerio (zebrafish)	cltcb	NP_001181907.1
Drosophila melanogaster (fruit fly)	Chc	NP_996451.1
Saccharomyces cerevisiae (baker's yeast)	CHC1	NP_011309.1
Arabidopsis thaliana (thale cress)	AT3G11130	NP_187724.2
Canis lupus familiaris (dog)	CLTC	XP_537700.2
Rattus norvegicus (Norway rat)	Cltc	NP_062172.1
Caenorhabditis elegans (roundworm)	chc-1	NP_499260.1
Neurospora crassa	NCU02510	XP_965641.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The clathrin heavy chain CHC-1 negatively regulates pulling forces acting on centrosomes during interphase and on spindle poles during mitosis in one-cell C. elegans embryos.
Title: Clathrin regulates centrosome positioning by promoting acto-myosin cortical tension in C. elegans embryos.

Inactivating epn-1 or chc-1 disrupts engulfment by impairing actin polymerization. CHC-1 is enriched on extending pseudopods together with EPN-1, in an EPN-1-dependent manner.
Title: Phagocytic receptor signaling regulates clathrin and epsin-mediated cytoskeletal remodeling during apoptotic cell engulfment in C. elegans.

Loss of SNX-1, RME-8, or the clathrin chaperone Hsc70/HSP-1 leads to over-accumulation of endosomal clathrin, reduced clathrin dynamics, and missorting of MIG-14 to the lysosome.
Title: Regulation of endosomal clathrin and retromer-mediated endosome to Golgi retrograde transport by the J-domain protein RME-8.

These results indicate that a high level of CHC activity is required for receptor-mediated endocytosis in nonneuronal cells but is largely dispensable for maintenance of synaptic vesicle pools.
Title: Differential requirements for clathrin in receptor-mediated endocytosis and maintenance of synaptic vesicle pools.

The following chc-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chc-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa341854	NM_066859.5 Latest version!	Caenorhabditis elegans Probable clathrin heavy chain 1 (chc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa341854	NM_066859.4	Caenorhabditis elegans Probable clathrin heavy chain 1 (chc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chc-1 ( NM_066859.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499260.1 Caenorhabditis elegans Probable clathrin heavy chain 1 (chc-1), partial mRNA.

Price & Availability↑

CloneID	OCa341854
Clone ID Related Accession (Same CDS sequence)	NM_066859.4 , NM_066859.5
Accession Version	NM_066859.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Probable clathrin heavy chain 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066859.3. COMPLETENESS: incomplete on both ends.

ATGGCGCTCC CAATCAAATT TCACGAGCAC CTGCAGCTTC CGAATGCTGG AATTCGGGTG 
CCCAACATTA CATTCTCCAA TGTCACAATG GAATCTGATA AGAACATTGT TGTTCGTGAG 
ATGATTGGTG ATCAACAACA GGTTGTGATT ATTGATTTGG CCGACACAGC GAATCCAACT 
CGTCGTCCAA TTTCTGCTGA CTCAGTAATC ATGCACCCAA CTGCCAAAAT TCTTGCTTTG 
AAATCTGGCA AGACACTTCA AATTTTCAAT ATTGAGTTGA AAGCAAAAGT GAAAGCTCAT 
CAGAATGTAG AAGATGTAGT TTATTGGAAG TGGATCAGTG AGAAAACGAT TGCACTCGTT 
TCCGACACAG CCGTCTATCA TTGGTCGATC GAGGGTGATG CAGCTCCAGT CAAGATGTTC 
GATAGACATC AGTCGTTGGC CGGAACTCAA ATCATAAATT ATCGCGCTGA TGCCGAAAAC 
AAGTGGCTCG TTCTTATTGG AATTTCCGCA AAAGACTCCC GTGTCGTTGG AAGTATGCAA 
TTGTACAGTA CCGAGAGAAA AGTATCGCAG CCAATCGAAG GCCATGCCGC TTGCTTCGTT 
CGATTCAAGG TCGATGGAAA CCAAAACCCA TCTAACTTAT TCTGTTTTTC CGTCAAAACC 
GATAATGGAG GAAAATTGCA CGTTATTGAA GTAGGAACCC CAGCTGCTGG AAACACTCCT 
TTCCAAAAGA AGAATGTTGA TGTTCCATAT ACCGCTGACA CAGCTGGCGA TTTTCCAGTG 
TCTATGCAGG TATCTGCGAA GCAAGGAATT ATTTACCTGG TAACAAAGCA AGGATATGTT 
CATCTTTATG ATGTCGAGTC TGGAACACGG ATTTATTCGA ATCGAATTTC GACTGATACC 
GTATTTGTGA CCTGTGAATA CACAGCTACA GGAGGAATAA TGGGAATTAA CAGAAAGGGA 
CAAGTTCTTT CCGTCTCTAT CGATGAAGCT AACTTGGTTC CGTTCGTTAC GAACCAGCTG 
CAAAATCCAG ATTTGGCTTT GAAGTTGGCC GTGCGTTGTG ATCTGCCTGG AGCTGAAGAG 
TTGTTCGTTC GCAAATTCAA CTTGTTGTTC AGCAACGGAC AGTTTGGAGA ATCAGCCAAG 
GTTGCTGCAT CGGCTCCACA AGGAATTTTG AGAACACCAG CCACCATTCA AAAGTTCCAG 
CAGTGCCCGT CTACTGGTCC AGGACCATCT CCACTTCTTC AATATTTCGG AATTCTTCTC 
GATCAAGGAA AGTTGAATAA ATACGAAACA CTCGAGCTGT GTCGTCCGGT CTTGGCACAA 
GGAAGAAAGG AGTTGATAAC GAAGTGGCTC AATGATCAGA AGTTGGAGTG TTGTGAAGAA 
TTGGGAGATC TCATTAAGCC GCACGATGTG AATACTGCTC TTTCAGTCTA TTTGCGTGGA 
AATGTCCCGC ACAAGGTTGT TCAATCTTTT GCTGAAACTG GACAATTCGA CAAGATCGTA 
ATGTATGCCA AGCGTGTCGG ATTCCAACCA GACTATTTGT TCCAACTTCG TCAAATTCTC 
CGTAATTCGA ATCCTGATCA TGGAGCAAAA TTCGCTCAAC TTCTTGTCTC TGAGAGCGAA 
AACGGAGAAC CACTTGCTGA TCTTTCACAG ATCATTGACT GTTTCATGGA GGTTCAAGCT 
GTTCAACCAT GCACTTCATT CCTTCTCGAA GTTTTGAAGG GTGATAAGCC GGAGGAAGGA 
CATCTGCAAA CGAGACTTCT TGAAATGAAT CTGCTCGCTG CCCCAGCTGT TGCCGATGCT 
ATTCTAGCCA ACAAGATGTT CTCTCACTAC GATCGTGCCG CTATCGGACA ACTCTGCGAA 
AAGGCTGGAC TTCTTCAAAG AGCTCTCGAA CACTTCACTG ACCTCTACGA CATTAAGCGA 
ACTGTTGTTC ACACCCATCT TCTCAAGCCT GATTGGCTCG TTGGATATTT CGGAAGCCTT 
TCTGTTGAAG ATTCGGTCGA ATGCTTGAAA GCCATGCTCA CTCAGAATAT CCGACAGAAC 
CTCCAAGTCG TTGTGCAGAT TGCATCCAAG TATCATGAAC AGCTCGGAGC TGACAAGCTG 
ATTGAGATGT TCGAAAATCA CAAGTCATAC GAAGGTCTTT TCTACTTCCT TGGTTCGATT 
GTCAACTTCA GCCAGGACCC AGAAGTTCAC TTCAAGTACA TTCAAGCTGC CACGCGCACA 
GGGCAAATTA AAGAGGTTGA GCGTATCTGT AGAGAGTCGC AATGTTATGA TGCCGAACGT 
GTCAAGAATT TCCTTAAGGA AGCCAAGCTC AACGATCAAC TTCCTTTGAT CATTGTCTGC 
GATCGTCATA ACATGGTTCA TGATCTTGTT CTTTACCTGT ACAGAAACCA GCTCCAGAAG 
TACATTGAAG TCTTTGTTCA AAAGGTTAAT GCCGCCCGTC TTCCGATCGT TGTTGGTGCT 
CTTCTAGATG TTGATTGTAG TGAAGATGCT ATCAAACAAT TGATCATTAA CACTCGTGGA 
AAGTTTGACA TTGATGAGCT TGTCGAGGAA GTTGAGAAAC GCAATCGTTT GAAGCTTTTG 
AATCACTGGC TTGAATCGAA AATCCAGGAG GGAGCGACTG ATGCTGCTAC CCACAATGCG 
ATGGCCAAGA TTTACATAGA TTCTAACAAC AATCCCGAAC GATTCTTGAA GGAAAATCCC 
TACTACGATA GCAAAGTTGT TGGAAAGTAC TGCGAAAAGC GTGATCCTCA CTACGCCTTC 
TTGTCATACG AACGCGGACA GTGCGACGCT GAATTGATCA ACGTGTGCAA CGAGAATTCA 
CTATTCAAGA ATCTTGCAAG ATATTTGGTG AAGAGAAGAG ACTTCACTCT GTGGGAGCAA 
GTTCTCAACG AAGAGAATGT TCATCGTAGA CAGCTGATAG ATCAAGTGGT TCAAACTGCT 
CTTTCCGAAA CTCAAGATCC CGAAGACATC TCTGTGACTG TTAAGGCATT CATGGCTGCC 
GATTTGCCGA ACGAACTTAT TGAACTTTTG GAAAAGATTG TTCTCGATAA CTCTGCATTC 
TCGGAGCACC GTAATCTTCA AAATCTGCTC ATTTTGACTG CAATGAGAGC CGATAGAACT 
CGTGTGATGG AGTACATTCA AAAGCTTGAC AACTATGATG CTCCAGATAT TGCTAACATT 
GCTATAACTT CTGAACTCTA CGAAGAAGCG TTTGCCATTT TCAAGAAATT CGATGTGAAC 
TCAAGTGCCA TCAATGTTCT TATCGAGAAC GTGAACAATT TGGATCGTGC TTACGAATTT 
GCTGAAAAGT GCAACCAGTC AGATGTCTGG GCATCTTTAG CCAAGGCACA GCTCCAACAA 
AATTTGGTGA AGGAGGCTGT CGATTCGTTT ATCAAGGCCG ATGATCCAGG AGCGTACATG 
GAGGTTGTGA ACAAATGTTC TCAAACGGAG CACTGGGAAG ATCTTGTTCG TTACCTTCAA 
ATGGCTCGCA AGAAGAGTCG TGAGTCATAC ATCGAAACGG AACTCGTTTT TGCTCTGGCC 
AAGACTGGTC GTCTCACTGA ACTTGAAGAG TTTATCGCTG GACCAAATCA CGCTCAAATC 
GGACAAATTG GAGATCGTTG CTTTGACAAT GGAATGTTCG ATTCCGCTAA GATCCTTTTC 
AACAATGTTT CCAACTTCGC CAAACTTTCC GTTACTCTTG TTCGTCTTGG AGAATATCAA 
GGAGCTGTCG ATGCTGCACG CAAAGCCAAC AGCACGAAGA CATGGAAACA AGTGTGCTTC 
TCTTGTGTCG AGAATGGAGA GTTCCGGCTT GCCCAGATGT GTGGACTTCA CATCGTTGTC 
CACGCTGATG AGCTTGAGGA ATTGATCAAC TTCTACCAAG ATCGTGGACA TTTCGAAGAG 
CTCATTGCTC TTCTCGAAGC TGCATTGGGT CTTGAACGGG CTCACATGGG AATGTTCACC 
GAACTCGCGA TTTTGTACTC GAAGTATAAG CCAGAGAAGA TGCGTGAGCA TTTGGAATTG 
TTCTGGAGCC GTGTTAATAT TCCAAAAGTT CTTCGTGCTG CCGAGCAGGC TCATCTTTGG 
TCCGAGTTGG TGTTCTTGTA CGATAAATAC GAAGAATATG ATAACGCCGC ACTGACAATG 
ATGCAGCATC CAACTGAATC TTGGAGAGAG CAACATTTCA AGGAAGTCAT CGCTAAAGTC 
GCTAATGTTG AGCTATACTA CAAAGCAATG CAGTTCTACC TGGATTATAA GCCACTTCTC 
CTGAATGACC TTCTTACTGT GCTATCGCCT CGGTTGGATC ATTCGCGCAC AGTTTTGTTC 
TTCAATAAGC TGAAGCAAAT CCCACTGGTC AAGCCTTACT TGAGACAAGT TCAAAACTTG 
AACAACAAGG CTATCAACGA GGCTTTGAAT CAGTTGCTCA TTGATGAGGA GGATCACGCC 
GGGCTTCGTT CTTCTATTGA GGCTCAAGAC AACTTTGACA ATATCACTTT GGCCCAACAA 
CTTGAGAAGC ACCCACTTGT GGAATTCAGA AGAATTTCCG CATACTTGTT CAAGGGAAAC 
AACAGATGGA AGCAATCGAT CGAGCTTTGC AAAAAAGACA AGCTGTACAA GGATGCCATG 
GAATACGCTG CAGAATCGAG AAACGGAGAA CTCGCCGAAG AGCTCTTGTC ATTCTTCCTG 
GATGAGAAAC TTTATGATTG TTTTGCCGCC TCCCTTTACC ATTGTTATGA TCTCCTTCAC 
CCCGATGTCA TCATGGAGCT CGCTTGGAAG CATAAGATTA TGGATTACGC CATGCCTTAC 
ATGATCCAAG TTATGAGAGA TTATCAGACG AGACTTGAGA AGTTGGAGCG TTCTGAGCAT 
GAGAGGAAAG AGGAGAAGGC TGAGCAGCAA CAAAACAATG GAATGACCAT GGAACCACAA 
CTTATGCTTA CCTATGGTGC CCCTGCCCCA CAAATGACTT ATCCAGGAAC AACTGGTGGC 
TATGGCGGAC AGCCTGCTTA CGGTCAACCT GGACAACCAG GATATAATGC CCCTGGATTT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499260.1
CDS	1..5046
Translation	MALPIKFHEHLQLPNAGIRVPNITFSNVTMESDKNIVVREMIGDQQQVVIIDLADTANPTRRPISADSVIMHPTAKILALKSGKTLQIFNIELKAKVKAHQNVEDVVYWKWISEKTIALVSDTAVYHWSIEGDAAPVKMFDRHQSLAGTQIINYRADAENKWLVLIGISAKDSRVVGSMQLYSTERKVSQPIEGHAACFVRFKVDGNQNPSNLFCFSVKTDNGGKLHVIEVGTPAAGNTPFQKKNVDVPYTADTAGDFPVSMQVSAKQGIIYLVTKQGYVHLYDVESGTRIYSNRISTDTVFVTCEYTATGGIMGINRKGQVLSVSIDEANLVPFVTNQLQNPDLALKLAVRCDLPGAEELFVRKFNLLFSNGQFGESAKVAASAPQGILRTPATIQKFQQCPSTGPGPSPLLQYFGILLDQGKLNKYETLELCRPVLAQGRKELITKWLNDQKLECCEELGDLIKPHDVNTALSVYLRGNVPHKVVQSFAETGQFDKIVMYAKRVGFQPDYLFQLRQILRNSNPDHGAKFAQLLVSESENGEPLADLSQIIDCFMEVQAVQPCTSFLLEVLKGDKPEEGHLQTRLLEMNLLAAPAVADAILANKMFSHYDRAAIGQLCEKAGLLQRALEHFTDLYDIKRTVVHTHLLKPDWLVGYFGSLSVEDSVECLKAMLTQNIRQNLQVVVQIASKYHEQLGADKLIEMFENHKSYEGLFYFLGSIVNFSQDPEVHFKYIQAATRTGQIKEVERICRESQCYDAERVKNFLKEAKLNDQLPLIIVCDRHNMVHDLVLYLYRNQLQKYIEVFVQKVNAARLPIVVGALLDVDCSEDAIKQLIINTRGKFDIDELVEEVEKRNRLKLLNHWLESKIQEGATDAATHNAMAKIYIDSNNNPERFLKENPYYDSKVVGKYCEKRDPHYAFLSYERGQCDAELINVCNENSLFKNLARYLVKRRDFTLWEQVLNEENVHRRQLIDQVVQTALSETQDPEDISVTVKAFMAADLPNELIELLEKIVLDNSAFSEHRNLQNLLILTAMRADRTRVMEYIQKLDNYDAPDIANIAITSELYEEAFAIFKKFDVNSSAINVLIENVNNLDRAYEFAEKCNQSDVWASLAKAQLQQNLVKEAVDSFIKADDPGAYMEVVNKCSQTEHWEDLVRYLQMARKKSRESYIETELVFALAKTGRLTELEEFIAGPNHAQIGQIGDRCFDNGMFDSAKILFNNVSNFAKLSVTLVRLGEYQGAVDAARKANSTKTWKQVCFSCVENGEFRLAQMCGLHIVVHADELEELINFYQDRGHFEELIALLEAALGLERAHMGMFTELAILYSKYKPEKMREHLELFWSRVNIPKVLRAAEQAHLWSELVFLYDKYEEYDNAALTMMQHPTESWREQHFKEVIAKVANVELYYKAMQFYLDYKPLLLNDLLTVLSPRLDHSRTVLFFNKLKQIPLVKPYLRQVQNLNNKAINEALNQLLIDEEDHAGLRSSIEAQDNFDNITLAQQLEKHPLVEFRRISAYLFKGNNRWKQSIELCKKDKLYKDAMEYAAESRNGELAEELLSFFLDEKLYDCFAASLYHCYDLLHPDVIMELAWKHKIMDYAMPYMIQVMRDYQTRLEKLERSEHERKEEKAEQQQNNGMTMEPQLMLTYGAPAPQMTYPGTTGGYGGQPAYGQPGQPGYNAPGFM

Target ORF information:

RefSeq Version	NM_066859.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Probable clathrin heavy chain 1 (chc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066859.4

ORF Insert Sequence:

ATGGCGCTCC CAATCAAATT TCACGAGCAC CTGCAGCTTC CGAATGCTGG AATTCGGGTG 
CCCAACATTA CATTCTCCAA TGTCACAATG GAATCTGATA AGAACATTGT TGTTCGTGAG 
ATGATTGGTG ATCAACAACA GGTTGTGATT ATTGATTTGG CCGACACAGC GAATCCAACT 
CGTCGTCCAA TTTCTGCTGA CTCAGTAATC ATGCACCCAA CTGCCAAAAT TCTTGCTTTG 
AAATCTGGCA AGACACTTCA AATTTTCAAT ATTGAGTTGA AAGCAAAAGT GAAAGCTCAT 
CAGAATGTAG AAGATGTAGT TTATTGGAAG TGGATCAGTG AGAAAACGAT TGCACTCGTT 
TCCGACACAG CCGTCTATCA TTGGTCGATC GAGGGTGATG CAGCTCCAGT CAAGATGTTC 
GATAGACATC AGTCGTTGGC CGGAACTCAA ATCATAAATT ATCGCGCTGA TGCCGAAAAC 
AAGTGGCTCG TTCTTATTGG AATTTCCGCA AAAGACTCCC GTGTCGTTGG AAGTATGCAA 
TTGTACAGTA CCGAGAGAAA AGTATCGCAG CCAATCGAAG GCCATGCCGC TTGCTTCGTT 
CGATTCAAGG TCGATGGAAA CCAAAACCCA TCTAACTTAT TCTGTTTTTC CGTCAAAACC 
GATAATGGAG GAAAATTGCA CGTTATTGAA GTAGGAACCC CAGCTGCTGG AAACACTCCT 
TTCCAAAAGA AGAATGTTGA TGTTCCATAT ACCGCTGACA CAGCTGGCGA TTTTCCAGTG 
TCTATGCAGG TATCTGCGAA GCAAGGAATT ATTTACCTGG TAACAAAGCA AGGATATGTT 
CATCTTTATG ATGTCGAGTC TGGAACACGG ATTTATTCGA ATCGAATTTC GACTGATACC 
GTATTTGTGA CCTGTGAATA CACAGCTACA GGAGGAATAA TGGGAATTAA CAGAAAGGGA 
CAAGTTCTTT CCGTCTCTAT CGATGAAGCT AACTTGGTTC CGTTCGTTAC GAACCAGCTG 
CAAAATCCAG ATTTGGCTTT GAAGTTGGCC GTGCGTTGTG ATCTGCCTGG AGCTGAAGAG 
TTGTTCGTTC GCAAATTCAA CTTGTTGTTC AGCAACGGAC AGTTTGGAGA ATCAGCCAAG 
GTTGCTGCAT CGGCTCCACA AGGAATTTTG AGAACACCAG CCACCATTCA AAAGTTCCAG 
CAGTGCCCGT CTACTGGTCC AGGACCATCT CCACTTCTTC AATATTTCGG AATTCTTCTC 
GATCAAGGAA AGTTGAATAA ATACGAAACA CTCGAGCTGT GTCGTCCGGT CTTGGCACAA 
GGAAGAAAGG AGTTGATAAC GAAGTGGCTC AATGATCAGA AGTTGGAGTG TTGTGAAGAA 
TTGGGAGATC TCATTAAGCC GCACGATGTG AATACTGCTC TTTCAGTCTA TTTGCGTGGA 
AATGTCCCGC ACAAGGTTGT TCAATCTTTT GCTGAAACTG GACAATTCGA CAAGATCGTA 
ATGTATGCCA AGCGTGTCGG ATTCCAACCA GACTATTTGT TCCAACTTCG TCAAATTCTC 
CGTAATTCGA ATCCTGATCA TGGAGCAAAA TTCGCTCAAC TTCTTGTCTC TGAGAGCGAA 
AACGGAGAAC CACTTGCTGA TCTTTCACAG ATCATTGACT GTTTCATGGA GGTTCAAGCT 
GTTCAACCAT GCACTTCATT CCTTCTCGAA GTTTTGAAGG GTGATAAGCC GGAGGAAGGA 
CATCTGCAAA CGAGACTTCT TGAAATGAAT CTGCTCGCTG CCCCAGCTGT TGCCGATGCT 
ATTCTAGCCA ACAAGATGTT CTCTCACTAC GATCGTGCCG CTATCGGACA ACTCTGCGAA 
AAGGCTGGAC TTCTTCAAAG AGCTCTCGAA CACTTCACTG ACCTCTACGA CATTAAGCGA 
ACTGTTGTTC ACACCCATCT TCTCAAGCCT GATTGGCTCG TTGGATATTT CGGAAGCCTT 
TCTGTTGAAG ATTCGGTCGA ATGCTTGAAA GCCATGCTCA CTCAGAATAT CCGACAGAAC 
CTCCAAGTCG TTGTGCAGAT TGCATCCAAG TATCATGAAC AGCTCGGAGC TGACAAGCTG 
ATTGAGATGT TCGAAAATCA CAAGTCATAC GAAGGTCTTT TCTACTTCCT TGGTTCGATT 
GTCAACTTCA GCCAGGACCC AGAAGTTCAC TTCAAGTACA TTCAAGCTGC CACGCGCACA 
GGGCAAATTA AAGAGGTTGA GCGTATCTGT AGAGAGTCGC AATGTTATGA TGCCGAACGT 
GTCAAGAATT TCCTTAAGGA AGCCAAGCTC AACGATCAAC TTCCTTTGAT CATTGTCTGC 
GATCGTCATA ACATGGTTCA TGATCTTGTT CTTTACCTGT ACAGAAACCA GCTCCAGAAG 
TACATTGAAG TCTTTGTTCA AAAGGTTAAT GCCGCCCGTC TTCCGATCGT TGTTGGTGCT 
CTTCTAGATG TTGATTGTAG TGAAGATGCT ATCAAACAAT TGATCATTAA CACTCGTGGA 
AAGTTTGACA TTGATGAGCT TGTCGAGGAA GTTGAGAAAC GCAATCGTTT GAAGCTTTTG 
AATCACTGGC TTGAATCGAA AATCCAGGAG GGAGCGACTG ATGCTGCTAC CCACAATGCG 
ATGGCCAAGA TTTACATAGA TTCTAACAAC AATCCCGAAC GATTCTTGAA GGAAAATCCC 
TACTACGATA GCAAAGTTGT TGGAAAGTAC TGCGAAAAGC GTGATCCTCA CTACGCCTTC 
TTGTCATACG AACGCGGACA GTGCGACGCT GAATTGATCA ACGTGTGCAA CGAGAATTCA 
CTATTCAAGA ATCTTGCAAG ATATTTGGTG AAGAGAAGAG ACTTCACTCT GTGGGAGCAA 
GTTCTCAACG AAGAGAATGT TCATCGTAGA CAGCTGATAG ATCAAGTGGT TCAAACTGCT 
CTTTCCGAAA CTCAAGATCC CGAAGACATC TCTGTGACTG TTAAGGCATT CATGGCTGCC 
GATTTGCCGA ACGAACTTAT TGAACTTTTG GAAAAGATTG TTCTCGATAA CTCTGCATTC 
TCGGAGCACC GTAATCTTCA AAATCTGCTC ATTTTGACTG CAATGAGAGC CGATAGAACT 
CGTGTGATGG AGTACATTCA AAAGCTTGAC AACTATGATG CTCCAGATAT TGCTAACATT 
GCTATAACTT CTGAACTCTA CGAAGAAGCG TTTGCCATTT TCAAGAAATT CGATGTGAAC 
TCAAGTGCCA TCAATGTTCT TATCGAGAAC GTGAACAATT TGGATCGTGC TTACGAATTT 
GCTGAAAAGT GCAACCAGTC AGATGTCTGG GCATCTTTAG CCAAGGCACA GCTCCAACAA 
AATTTGGTGA AGGAGGCTGT CGATTCGTTT ATCAAGGCCG ATGATCCAGG AGCGTACATG 
GAGGTTGTGA ACAAATGTTC TCAAACGGAG CACTGGGAAG ATCTTGTTCG TTACCTTCAA 
ATGGCTCGCA AGAAGAGTCG TGAGTCATAC ATCGAAACGG AACTCGTTTT TGCTCTGGCC 
AAGACTGGTC GTCTCACTGA ACTTGAAGAG TTTATCGCTG GACCAAATCA CGCTCAAATC 
GGACAAATTG GAGATCGTTG CTTTGACAAT GGAATGTTCG ATTCCGCTAA GATCCTTTTC 
AACAATGTTT CCAACTTCGC CAAACTTTCC GTTACTCTTG TTCGTCTTGG AGAATATCAA 
GGAGCTGTCG ATGCTGCACG CAAAGCCAAC AGCACGAAGA CATGGAAACA AGTGTGCTTC 
TCTTGTGTCG AGAATGGAGA GTTCCGGCTT GCCCAGATGT GTGGACTTCA CATCGTTGTC 
CACGCTGATG AGCTTGAGGA ATTGATCAAC TTCTACCAAG ATCGTGGACA TTTCGAAGAG 
CTCATTGCTC TTCTCGAAGC TGCATTGGGT CTTGAACGGG CTCACATGGG AATGTTCACC 
GAACTCGCGA TTTTGTACTC GAAGTATAAG CCAGAGAAGA TGCGTGAGCA TTTGGAATTG 
TTCTGGAGCC GTGTTAATAT TCCAAAAGTT CTTCGTGCTG CCGAGCAGGC TCATCTTTGG 
TCCGAGTTGG TGTTCTTGTA CGATAAATAC GAAGAATATG ATAACGCCGC ACTGACAATG 
ATGCAGCATC CAACTGAATC TTGGAGAGAG CAACATTTCA AGGAAGTCAT CGCTAAAGTC 
GCTAATGTTG AGCTATACTA CAAAGCAATG CAGTTCTACC TGGATTATAA GCCACTTCTC 
CTGAATGACC TTCTTACTGT GCTATCGCCT CGGTTGGATC ATTCGCGCAC AGTTTTGTTC 
TTCAATAAGC TGAAGCAAAT CCCACTGGTC AAGCCTTACT TGAGACAAGT TCAAAACTTG 
AACAACAAGG CTATCAACGA GGCTTTGAAT CAGTTGCTCA TTGATGAGGA GGATCACGCC 
GGGCTTCGTT CTTCTATTGA GGCTCAAGAC AACTTTGACA ATATCACTTT GGCCCAACAA 
CTTGAGAAGC ACCCACTTGT GGAATTCAGA AGAATTTCCG CATACTTGTT CAAGGGAAAC 
AACAGATGGA AGCAATCGAT CGAGCTTTGC AAAAAAGACA AGCTGTACAA GGATGCCATG 
GAATACGCTG CAGAATCGAG AAACGGAGAA CTCGCCGAAG AGCTCTTGTC ATTCTTCCTG 
GATGAGAAAC TTTATGATTG TTTTGCCGCC TCCCTTTACC ATTGTTATGA TCTCCTTCAC 
CCCGATGTCA TCATGGAGCT CGCTTGGAAG CATAAGATTA TGGATTACGC CATGCCTTAC 
ATGATCCAAG TTATGAGAGA TTATCAGACG AGACTTGAGA AGTTGGAGCG TTCTGAGCAT 
GAGAGGAAAG AGGAGAAGGC TGAGCAGCAA CAAAACAATG GAATGACCAT GGAACCACAA 
CTTATGCTTA CCTATGGTGC CCCTGCCCCA CAAATGACTT ATCCAGGAAC AACTGGTGGC 
TATGGCGGAC AGCCTGCTTA CGGTCAACCT GGACAACCAG GATATAATGC CCCTGGATTT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chc-1 ( NM_066859.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_499260.1 Caenorhabditis elegans Probable clathrin heavy chain 1 (chc-1), mRNA.

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CloneID	OCa341854
Clone ID Related Accession (Same CDS sequence)	NM_066859.4 , NM_066859.5
Accession Version	NM_066859.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Probable clathrin heavy chain 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066859.4.

ATGGCGCTCC CAATCAAATT TCACGAGCAC CTGCAGCTTC CGAATGCTGG AATTCGGGTG 
CCCAACATTA CATTCTCCAA TGTCACAATG GAATCTGATA AGAACATTGT TGTTCGTGAG 
ATGATTGGTG ATCAACAACA GGTTGTGATT ATTGATTTGG CCGACACAGC GAATCCAACT 
CGTCGTCCAA TTTCTGCTGA CTCAGTAATC ATGCACCCAA CTGCCAAAAT TCTTGCTTTG 
AAATCTGGCA AGACACTTCA AATTTTCAAT ATTGAGTTGA AAGCAAAAGT GAAAGCTCAT 
CAGAATGTAG AAGATGTAGT TTATTGGAAG TGGATCAGTG AGAAAACGAT TGCACTCGTT 
TCCGACACAG CCGTCTATCA TTGGTCGATC GAGGGTGATG CAGCTCCAGT CAAGATGTTC 
GATAGACATC AGTCGTTGGC CGGAACTCAA ATCATAAATT ATCGCGCTGA TGCCGAAAAC 
AAGTGGCTCG TTCTTATTGG AATTTCCGCA AAAGACTCCC GTGTCGTTGG AAGTATGCAA 
TTGTACAGTA CCGAGAGAAA AGTATCGCAG CCAATCGAAG GCCATGCCGC TTGCTTCGTT 
CGATTCAAGG TCGATGGAAA CCAAAACCCA TCTAACTTAT TCTGTTTTTC CGTCAAAACC 
GATAATGGAG GAAAATTGCA CGTTATTGAA GTAGGAACCC CAGCTGCTGG AAACACTCCT 
TTCCAAAAGA AGAATGTTGA TGTTCCATAT ACCGCTGACA CAGCTGGCGA TTTTCCAGTG 
TCTATGCAGG TATCTGCGAA GCAAGGAATT ATTTACCTGG TAACAAAGCA AGGATATGTT 
CATCTTTATG ATGTCGAGTC TGGAACACGG ATTTATTCGA ATCGAATTTC GACTGATACC 
GTATTTGTGA CCTGTGAATA CACAGCTACA GGAGGAATAA TGGGAATTAA CAGAAAGGGA 
CAAGTTCTTT CCGTCTCTAT CGATGAAGCT AACTTGGTTC CGTTCGTTAC GAACCAGCTG 
CAAAATCCAG ATTTGGCTTT GAAGTTGGCC GTGCGTTGTG ATCTGCCTGG AGCTGAAGAG 
TTGTTCGTTC GCAAATTCAA CTTGTTGTTC AGCAACGGAC AGTTTGGAGA ATCAGCCAAG 
GTTGCTGCAT CGGCTCCACA AGGAATTTTG AGAACACCAG CCACCATTCA AAAGTTCCAG 
CAGTGCCCGT CTACTGGTCC AGGACCATCT CCACTTCTTC AATATTTCGG AATTCTTCTC 
GATCAAGGAA AGTTGAATAA ATACGAAACA CTCGAGCTGT GTCGTCCGGT CTTGGCACAA 
GGAAGAAAGG AGTTGATAAC GAAGTGGCTC AATGATCAGA AGTTGGAGTG TTGTGAAGAA 
TTGGGAGATC TCATTAAGCC GCACGATGTG AATACTGCTC TTTCAGTCTA TTTGCGTGGA 
AATGTCCCGC ACAAGGTTGT TCAATCTTTT GCTGAAACTG GACAATTCGA CAAGATCGTA 
ATGTATGCCA AGCGTGTCGG ATTCCAACCA GACTATTTGT TCCAACTTCG TCAAATTCTC 
CGTAATTCGA ATCCTGATCA TGGAGCAAAA TTCGCTCAAC TTCTTGTCTC TGAGAGCGAA 
AACGGAGAAC CACTTGCTGA TCTTTCACAG ATCATTGACT GTTTCATGGA GGTTCAAGCT 
GTTCAACCAT GCACTTCATT CCTTCTCGAA GTTTTGAAGG GTGATAAGCC GGAGGAAGGA 
CATCTGCAAA CGAGACTTCT TGAAATGAAT CTGCTCGCTG CCCCAGCTGT TGCCGATGCT 
ATTCTAGCCA ACAAGATGTT CTCTCACTAC GATCGTGCCG CTATCGGACA ACTCTGCGAA 
AAGGCTGGAC TTCTTCAAAG AGCTCTCGAA CACTTCACTG ACCTCTACGA CATTAAGCGA 
ACTGTTGTTC ACACCCATCT TCTCAAGCCT GATTGGCTCG TTGGATATTT CGGAAGCCTT 
TCTGTTGAAG ATTCGGTCGA ATGCTTGAAA GCCATGCTCA CTCAGAATAT CCGACAGAAC 
CTCCAAGTCG TTGTGCAGAT TGCATCCAAG TATCATGAAC AGCTCGGAGC TGACAAGCTG 
ATTGAGATGT TCGAAAATCA CAAGTCATAC GAAGGTCTTT TCTACTTCCT TGGTTCGATT 
GTCAACTTCA GCCAGGACCC AGAAGTTCAC TTCAAGTACA TTCAAGCTGC CACGCGCACA 
GGGCAAATTA AAGAGGTTGA GCGTATCTGT AGAGAGTCGC AATGTTATGA TGCCGAACGT 
GTCAAGAATT TCCTTAAGGA AGCCAAGCTC AACGATCAAC TTCCTTTGAT CATTGTCTGC 
GATCGTCATA ACATGGTTCA TGATCTTGTT CTTTACCTGT ACAGAAACCA GCTCCAGAAG 
TACATTGAAG TCTTTGTTCA AAAGGTTAAT GCCGCCCGTC TTCCGATCGT TGTTGGTGCT 
CTTCTAGATG TTGATTGTAG TGAAGATGCT ATCAAACAAT TGATCATTAA CACTCGTGGA 
AAGTTTGACA TTGATGAGCT TGTCGAGGAA GTTGAGAAAC GCAATCGTTT GAAGCTTTTG 
AATCACTGGC TTGAATCGAA AATCCAGGAG GGAGCGACTG ATGCTGCTAC CCACAATGCG 
ATGGCCAAGA TTTACATAGA TTCTAACAAC AATCCCGAAC GATTCTTGAA GGAAAATCCC 
TACTACGATA GCAAAGTTGT TGGAAAGTAC TGCGAAAAGC GTGATCCTCA CTACGCCTTC 
TTGTCATACG AACGCGGACA GTGCGACGCT GAATTGATCA ACGTGTGCAA CGAGAATTCA 
CTATTCAAGA ATCTTGCAAG ATATTTGGTG AAGAGAAGAG ACTTCACTCT GTGGGAGCAA 
GTTCTCAACG AAGAGAATGT TCATCGTAGA CAGCTGATAG ATCAAGTGGT TCAAACTGCT 
CTTTCCGAAA CTCAAGATCC CGAAGACATC TCTGTGACTG TTAAGGCATT CATGGCTGCC 
GATTTGCCGA ACGAACTTAT TGAACTTTTG GAAAAGATTG TTCTCGATAA CTCTGCATTC 
TCGGAGCACC GTAATCTTCA AAATCTGCTC ATTTTGACTG CAATGAGAGC CGATAGAACT 
CGTGTGATGG AGTACATTCA AAAGCTTGAC AACTATGATG CTCCAGATAT TGCTAACATT 
GCTATAACTT CTGAACTCTA CGAAGAAGCG TTTGCCATTT TCAAGAAATT CGATGTGAAC 
TCAAGTGCCA TCAATGTTCT TATCGAGAAC GTGAACAATT TGGATCGTGC TTACGAATTT 
GCTGAAAAGT GCAACCAGTC AGATGTCTGG GCATCTTTAG CCAAGGCACA GCTCCAACAA 
AATTTGGTGA AGGAGGCTGT CGATTCGTTT ATCAAGGCCG ATGATCCAGG AGCGTACATG 
GAGGTTGTGA ACAAATGTTC TCAAACGGAG CACTGGGAAG ATCTTGTTCG TTACCTTCAA 
ATGGCTCGCA AGAAGAGTCG TGAGTCATAC ATCGAAACGG AACTCGTTTT TGCTCTGGCC 
AAGACTGGTC GTCTCACTGA ACTTGAAGAG TTTATCGCTG GACCAAATCA CGCTCAAATC 
GGACAAATTG GAGATCGTTG CTTTGACAAT GGAATGTTCG ATTCCGCTAA GATCCTTTTC 
AACAATGTTT CCAACTTCGC CAAACTTTCC GTTACTCTTG TTCGTCTTGG AGAATATCAA 
GGAGCTGTCG ATGCTGCACG CAAAGCCAAC AGCACGAAGA CATGGAAACA AGTGTGCTTC 
TCTTGTGTCG AGAATGGAGA GTTCCGGCTT GCCCAGATGT GTGGACTTCA CATCGTTGTC 
CACGCTGATG AGCTTGAGGA ATTGATCAAC TTCTACCAAG ATCGTGGACA TTTCGAAGAG 
CTCATTGCTC TTCTCGAAGC TGCATTGGGT CTTGAACGGG CTCACATGGG AATGTTCACC 
GAACTCGCGA TTTTGTACTC GAAGTATAAG CCAGAGAAGA TGCGTGAGCA TTTGGAATTG 
TTCTGGAGCC GTGTTAATAT TCCAAAAGTT CTTCGTGCTG CCGAGCAGGC TCATCTTTGG 
TCCGAGTTGG TGTTCTTGTA CGATAAATAC GAAGAATATG ATAACGCCGC ACTGACAATG 
ATGCAGCATC CAACTGAATC TTGGAGAGAG CAACATTTCA AGGAAGTCAT CGCTAAAGTC 
GCTAATGTTG AGCTATACTA CAAAGCAATG CAGTTCTACC TGGATTATAA GCCACTTCTC 
CTGAATGACC TTCTTACTGT GCTATCGCCT CGGTTGGATC ATTCGCGCAC AGTTTTGTTC 
TTCAATAAGC TGAAGCAAAT CCCACTGGTC AAGCCTTACT TGAGACAAGT TCAAAACTTG 
AACAACAAGG CTATCAACGA GGCTTTGAAT CAGTTGCTCA TTGATGAGGA GGATCACGCC 
GGGCTTCGTT CTTCTATTGA GGCTCAAGAC AACTTTGACA ATATCACTTT GGCCCAACAA 
CTTGAGAAGC ACCCACTTGT GGAATTCAGA AGAATTTCCG CATACTTGTT CAAGGGAAAC 
AACAGATGGA AGCAATCGAT CGAGCTTTGC AAAAAAGACA AGCTGTACAA GGATGCCATG 
GAATACGCTG CAGAATCGAG AAACGGAGAA CTCGCCGAAG AGCTCTTGTC ATTCTTCCTG 
GATGAGAAAC TTTATGATTG TTTTGCCGCC TCCCTTTACC ATTGTTATGA TCTCCTTCAC 
CCCGATGTCA TCATGGAGCT CGCTTGGAAG CATAAGATTA TGGATTACGC CATGCCTTAC 
ATGATCCAAG TTATGAGAGA TTATCAGACG AGACTTGAGA AGTTGGAGCG TTCTGAGCAT 
GAGAGGAAAG AGGAGAAGGC TGAGCAGCAA CAAAACAATG GAATGACCAT GGAACCACAA 
CTTATGCTTA CCTATGGTGC CCCTGCCCCA CAAATGACTT ATCCAGGAAC AACTGGTGGC 
TATGGCGGAC AGCCTGCTTA CGGTCAACCT GGACAACCAG GATATAATGC CCCTGGATTT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_499260.1
CDS	52..5097
Translation	MALPIKFHEHLQLPNAGIRVPNITFSNVTMESDKNIVVREMIGDQQQVVIIDLADTANPTRRPISADSVIMHPTAKILALKSGKTLQIFNIELKAKVKAHQNVEDVVYWKWISEKTIALVSDTAVYHWSIEGDAAPVKMFDRHQSLAGTQIINYRADAENKWLVLIGISAKDSRVVGSMQLYSTERKVSQPIEGHAACFVRFKVDGNQNPSNLFCFSVKTDNGGKLHVIEVGTPAAGNTPFQKKNVDVPYTADTAGDFPVSMQVSAKQGIIYLVTKQGYVHLYDVESGTRIYSNRISTDTVFVTCEYTATGGIMGINRKGQVLSVSIDEANLVPFVTNQLQNPDLALKLAVRCDLPGAEELFVRKFNLLFSNGQFGESAKVAASAPQGILRTPATIQKFQQCPSTGPGPSPLLQYFGILLDQGKLNKYETLELCRPVLAQGRKELITKWLNDQKLECCEELGDLIKPHDVNTALSVYLRGNVPHKVVQSFAETGQFDKIVMYAKRVGFQPDYLFQLRQILRNSNPDHGAKFAQLLVSESENGEPLADLSQIIDCFMEVQAVQPCTSFLLEVLKGDKPEEGHLQTRLLEMNLLAAPAVADAILANKMFSHYDRAAIGQLCEKAGLLQRALEHFTDLYDIKRTVVHTHLLKPDWLVGYFGSLSVEDSVECLKAMLTQNIRQNLQVVVQIASKYHEQLGADKLIEMFENHKSYEGLFYFLGSIVNFSQDPEVHFKYIQAATRTGQIKEVERICRESQCYDAERVKNFLKEAKLNDQLPLIIVCDRHNMVHDLVLYLYRNQLQKYIEVFVQKVNAARLPIVVGALLDVDCSEDAIKQLIINTRGKFDIDELVEEVEKRNRLKLLNHWLESKIQEGATDAATHNAMAKIYIDSNNNPERFLKENPYYDSKVVGKYCEKRDPHYAFLSYERGQCDAELINVCNENSLFKNLARYLVKRRDFTLWEQVLNEENVHRRQLIDQVVQTALSETQDPEDISVTVKAFMAADLPNELIELLEKIVLDNSAFSEHRNLQNLLILTAMRADRTRVMEYIQKLDNYDAPDIANIAITSELYEEAFAIFKKFDVNSSAINVLIENVNNLDRAYEFAEKCNQSDVWASLAKAQLQQNLVKEAVDSFIKADDPGAYMEVVNKCSQTEHWEDLVRYLQMARKKSRESYIETELVFALAKTGRLTELEEFIAGPNHAQIGQIGDRCFDNGMFDSAKILFNNVSNFAKLSVTLVRLGEYQGAVDAARKANSTKTWKQVCFSCVENGEFRLAQMCGLHIVVHADELEELINFYQDRGHFEELIALLEAALGLERAHMGMFTELAILYSKYKPEKMREHLELFWSRVNIPKVLRAAEQAHLWSELVFLYDKYEEYDNAALTMMQHPTESWREQHFKEVIAKVANVELYYKAMQFYLDYKPLLLNDLLTVLSPRLDHSRTVLFFNKLKQIPLVKPYLRQVQNLNNKAINEALNQLLIDEEDHAGLRSSIEAQDNFDNITLAQQLEKHPLVEFRRISAYLFKGNNRWKQSIELCKKDKLYKDAMEYAAESRNGELAEELLSFFLDEKLYDCFAASLYHCYDLLHPDVIMELAWKHKIMDYAMPYMIQVMRDYQTRLEKLERSEHERKEEKAEQQQNNGMTMEPQLMLTYGAPAPQMTYPGTTGGYGGQPAYGQPGQPGYNAPGFM

Target ORF information:

RefSeq Version	NM_066859.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Probable clathrin heavy chain 1 (chc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066859.5

ORF Insert Sequence:

ATGGCGCTCC CAATCAAATT TCACGAGCAC CTGCAGCTTC CGAATGCTGG AATTCGGGTG 
CCCAACATTA CATTCTCCAA TGTCACAATG GAATCTGATA AGAACATTGT TGTTCGTGAG 
ATGATTGGTG ATCAACAACA GGTTGTGATT ATTGATTTGG CCGACACAGC GAATCCAACT 
CGTCGTCCAA TTTCTGCTGA CTCAGTAATC ATGCACCCAA CTGCCAAAAT TCTTGCTTTG 
AAATCTGGCA AGACACTTCA AATTTTCAAT ATTGAGTTGA AAGCAAAAGT GAAAGCTCAT 
CAGAATGTAG AAGATGTAGT TTATTGGAAG TGGATCAGTG AGAAAACGAT TGCACTCGTT 
TCCGACACAG CCGTCTATCA TTGGTCGATC GAGGGTGATG CAGCTCCAGT CAAGATGTTC 
GATAGACATC AGTCGTTGGC CGGAACTCAA ATCATAAATT ATCGCGCTGA TGCCGAAAAC 
AAGTGGCTCG TTCTTATTGG AATTTCCGCA AAAGACTCCC GTGTCGTTGG AAGTATGCAA 
TTGTACAGTA CCGAGAGAAA AGTATCGCAG CCAATCGAAG GCCATGCCGC TTGCTTCGTT 
CGATTCAAGG TCGATGGAAA CCAAAACCCA TCTAACTTAT TCTGTTTTTC CGTCAAAACC 
GATAATGGAG GAAAATTGCA CGTTATTGAA GTAGGAACCC CAGCTGCTGG AAACACTCCT 
TTCCAAAAGA AGAATGTTGA TGTTCCATAT ACCGCTGACA CAGCTGGCGA TTTTCCAGTG 
TCTATGCAGG TATCTGCGAA GCAAGGAATT ATTTACCTGG TAACAAAGCA AGGATATGTT 
CATCTTTATG ATGTCGAGTC TGGAACACGG ATTTATTCGA ATCGAATTTC GACTGATACC 
GTATTTGTGA CCTGTGAATA CACAGCTACA GGAGGAATAA TGGGAATTAA CAGAAAGGGA 
CAAGTTCTTT CCGTCTCTAT CGATGAAGCT AACTTGGTTC CGTTCGTTAC GAACCAGCTG 
CAAAATCCAG ATTTGGCTTT GAAGTTGGCC GTGCGTTGTG ATCTGCCTGG AGCTGAAGAG 
TTGTTCGTTC GCAAATTCAA CTTGTTGTTC AGCAACGGAC AGTTTGGAGA ATCAGCCAAG 
GTTGCTGCAT CGGCTCCACA AGGAATTTTG AGAACACCAG CCACCATTCA AAAGTTCCAG 
CAGTGCCCGT CTACTGGTCC AGGACCATCT CCACTTCTTC AATATTTCGG AATTCTTCTC 
GATCAAGGAA AGTTGAATAA ATACGAAACA CTCGAGCTGT GTCGTCCGGT CTTGGCACAA 
GGAAGAAAGG AGTTGATAAC GAAGTGGCTC AATGATCAGA AGTTGGAGTG TTGTGAAGAA 
TTGGGAGATC TCATTAAGCC GCACGATGTG AATACTGCTC TTTCAGTCTA TTTGCGTGGA 
AATGTCCCGC ACAAGGTTGT TCAATCTTTT GCTGAAACTG GACAATTCGA CAAGATCGTA 
ATGTATGCCA AGCGTGTCGG ATTCCAACCA GACTATTTGT TCCAACTTCG TCAAATTCTC 
CGTAATTCGA ATCCTGATCA TGGAGCAAAA TTCGCTCAAC TTCTTGTCTC TGAGAGCGAA 
AACGGAGAAC CACTTGCTGA TCTTTCACAG ATCATTGACT GTTTCATGGA GGTTCAAGCT 
GTTCAACCAT GCACTTCATT CCTTCTCGAA GTTTTGAAGG GTGATAAGCC GGAGGAAGGA 
CATCTGCAAA CGAGACTTCT TGAAATGAAT CTGCTCGCTG CCCCAGCTGT TGCCGATGCT 
ATTCTAGCCA ACAAGATGTT CTCTCACTAC GATCGTGCCG CTATCGGACA ACTCTGCGAA 
AAGGCTGGAC TTCTTCAAAG AGCTCTCGAA CACTTCACTG ACCTCTACGA CATTAAGCGA 
ACTGTTGTTC ACACCCATCT TCTCAAGCCT GATTGGCTCG TTGGATATTT CGGAAGCCTT 
TCTGTTGAAG ATTCGGTCGA ATGCTTGAAA GCCATGCTCA CTCAGAATAT CCGACAGAAC 
CTCCAAGTCG TTGTGCAGAT TGCATCCAAG TATCATGAAC AGCTCGGAGC TGACAAGCTG 
ATTGAGATGT TCGAAAATCA CAAGTCATAC GAAGGTCTTT TCTACTTCCT TGGTTCGATT 
GTCAACTTCA GCCAGGACCC AGAAGTTCAC TTCAAGTACA TTCAAGCTGC CACGCGCACA 
GGGCAAATTA AAGAGGTTGA GCGTATCTGT AGAGAGTCGC AATGTTATGA TGCCGAACGT 
GTCAAGAATT TCCTTAAGGA AGCCAAGCTC AACGATCAAC TTCCTTTGAT CATTGTCTGC 
GATCGTCATA ACATGGTTCA TGATCTTGTT CTTTACCTGT ACAGAAACCA GCTCCAGAAG 
TACATTGAAG TCTTTGTTCA AAAGGTTAAT GCCGCCCGTC TTCCGATCGT TGTTGGTGCT 
CTTCTAGATG TTGATTGTAG TGAAGATGCT ATCAAACAAT TGATCATTAA CACTCGTGGA 
AAGTTTGACA TTGATGAGCT TGTCGAGGAA GTTGAGAAAC GCAATCGTTT GAAGCTTTTG 
AATCACTGGC TTGAATCGAA AATCCAGGAG GGAGCGACTG ATGCTGCTAC CCACAATGCG 
ATGGCCAAGA TTTACATAGA TTCTAACAAC AATCCCGAAC GATTCTTGAA GGAAAATCCC 
TACTACGATA GCAAAGTTGT TGGAAAGTAC TGCGAAAAGC GTGATCCTCA CTACGCCTTC 
TTGTCATACG AACGCGGACA GTGCGACGCT GAATTGATCA ACGTGTGCAA CGAGAATTCA 
CTATTCAAGA ATCTTGCAAG ATATTTGGTG AAGAGAAGAG ACTTCACTCT GTGGGAGCAA 
GTTCTCAACG AAGAGAATGT TCATCGTAGA CAGCTGATAG ATCAAGTGGT TCAAACTGCT 
CTTTCCGAAA CTCAAGATCC CGAAGACATC TCTGTGACTG TTAAGGCATT CATGGCTGCC 
GATTTGCCGA ACGAACTTAT TGAACTTTTG GAAAAGATTG TTCTCGATAA CTCTGCATTC 
TCGGAGCACC GTAATCTTCA AAATCTGCTC ATTTTGACTG CAATGAGAGC CGATAGAACT 
CGTGTGATGG AGTACATTCA AAAGCTTGAC AACTATGATG CTCCAGATAT TGCTAACATT 
GCTATAACTT CTGAACTCTA CGAAGAAGCG TTTGCCATTT TCAAGAAATT CGATGTGAAC 
TCAAGTGCCA TCAATGTTCT TATCGAGAAC GTGAACAATT TGGATCGTGC TTACGAATTT 
GCTGAAAAGT GCAACCAGTC AGATGTCTGG GCATCTTTAG CCAAGGCACA GCTCCAACAA 
AATTTGGTGA AGGAGGCTGT CGATTCGTTT ATCAAGGCCG ATGATCCAGG AGCGTACATG 
GAGGTTGTGA ACAAATGTTC TCAAACGGAG CACTGGGAAG ATCTTGTTCG TTACCTTCAA 
ATGGCTCGCA AGAAGAGTCG TGAGTCATAC ATCGAAACGG AACTCGTTTT TGCTCTGGCC 
AAGACTGGTC GTCTCACTGA ACTTGAAGAG TTTATCGCTG GACCAAATCA CGCTCAAATC 
GGACAAATTG GAGATCGTTG CTTTGACAAT GGAATGTTCG ATTCCGCTAA GATCCTTTTC 
AACAATGTTT CCAACTTCGC CAAACTTTCC GTTACTCTTG TTCGTCTTGG AGAATATCAA 
GGAGCTGTCG ATGCTGCACG CAAAGCCAAC AGCACGAAGA CATGGAAACA AGTGTGCTTC 
TCTTGTGTCG AGAATGGAGA GTTCCGGCTT GCCCAGATGT GTGGACTTCA CATCGTTGTC 
CACGCTGATG AGCTTGAGGA ATTGATCAAC TTCTACCAAG ATCGTGGACA TTTCGAAGAG 
CTCATTGCTC TTCTCGAAGC TGCATTGGGT CTTGAACGGG CTCACATGGG AATGTTCACC 
GAACTCGCGA TTTTGTACTC GAAGTATAAG CCAGAGAAGA TGCGTGAGCA TTTGGAATTG 
TTCTGGAGCC GTGTTAATAT TCCAAAAGTT CTTCGTGCTG CCGAGCAGGC TCATCTTTGG 
TCCGAGTTGG TGTTCTTGTA CGATAAATAC GAAGAATATG ATAACGCCGC ACTGACAATG 
ATGCAGCATC CAACTGAATC TTGGAGAGAG CAACATTTCA AGGAAGTCAT CGCTAAAGTC 
GCTAATGTTG AGCTATACTA CAAAGCAATG CAGTTCTACC TGGATTATAA GCCACTTCTC 
CTGAATGACC TTCTTACTGT GCTATCGCCT CGGTTGGATC ATTCGCGCAC AGTTTTGTTC 
TTCAATAAGC TGAAGCAAAT CCCACTGGTC AAGCCTTACT TGAGACAAGT TCAAAACTTG 
AACAACAAGG CTATCAACGA GGCTTTGAAT CAGTTGCTCA TTGATGAGGA GGATCACGCC 
GGGCTTCGTT CTTCTATTGA GGCTCAAGAC AACTTTGACA ATATCACTTT GGCCCAACAA 
CTTGAGAAGC ACCCACTTGT GGAATTCAGA AGAATTTCCG CATACTTGTT CAAGGGAAAC 
AACAGATGGA AGCAATCGAT CGAGCTTTGC AAAAAAGACA AGCTGTACAA GGATGCCATG 
GAATACGCTG CAGAATCGAG AAACGGAGAA CTCGCCGAAG AGCTCTTGTC ATTCTTCCTG 
GATGAGAAAC TTTATGATTG TTTTGCCGCC TCCCTTTACC ATTGTTATGA TCTCCTTCAC 
CCCGATGTCA TCATGGAGCT CGCTTGGAAG CATAAGATTA TGGATTACGC CATGCCTTAC 
ATGATCCAAG TTATGAGAGA TTATCAGACG AGACTTGAGA AGTTGGAGCG TTCTGAGCAT 
GAGAGGAAAG AGGAGAAGGC TGAGCAGCAA CAAAACAATG GAATGACCAT GGAACCACAA 
CTTATGCTTA CCTATGGTGC CCCTGCCCCA CAAATGACTT ATCCAGGAAC AACTGGTGGC 
TATGGCGGAC AGCCTGCTTA CGGTCAACCT GGACAACCAG GATATAATGC CCCTGGATTT 
ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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chc-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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