met-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	met-2
Entrez Gene ID	176183
Full Name	Histone-lysine N-methyltransferase met-2
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_066447.5	NP_498848.4	Histone-lysine N-methyltransferase met-2
NM_066447.6	NP_498848.4	Histone-lysine N-methyltransferase met-2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

both histone H3 lysine 9 di- and trimethylation were reduced in Cbr-met-2 mutant germ lines, suggesting that in contrast to C. elegans
Title: Plasticity in the Meiotic Epigenetic Landscape of Sex Chromosomes in Caenorhabditis Species.

SPR-5 and MET-2 function cooperatively to reestablish an epigenetic ground state during passage through the germ line.
Title: SPR-5 and MET-2 function cooperatively to reestablish an epigenetic ground state during passage through the germ line.

Study shows that MET-2, a SETDB1 homolog, deposits mono- and dimethyl groups at H3K9, whereas SET-25, a previously uncharacterized histone methyltransferases, trimethylates the same residue.
Title: Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery.

MET-2-dependent H3K9me2 has distinct outputs on the unpaired X chromosome versus asynapsed homologous chromosomes with respect to both checkpoint signaling and transcriptional silencing.
Title: Caenorhabditis elegans histone methyltransferase MET-2 shields the male X chromosome from checkpoint machinery and mediates meiotic sex chromosome inactivation.

Two C. elegans histone methyltransferases repress lin-3 EGF transcription to inhibit vulval development.
Title: Two C. elegans histone methyltransferases repress lin-3 EGF transcription to inhibit vulval development.

The following met-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the met-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340049	NM_066447.6 Latest version!	Caenorhabditis elegans Histone-lysine N-methyltransferase met-2 (met-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCa340049	NM_066447.5	Caenorhabditis elegans Histone-lysine N-methyltransferase met-2 (met-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

met-2 ( NM_066447.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498848.4 Caenorhabditis elegans Histone-lysine N-methyltransferase met-2 (met-2), partial mRNA.

Price & Availability↑

CloneID	OCa340049
Clone ID Related Accession (Same CDS sequence)	NM_066447.5 , NM_066447.6
Accession Version	NM_066447.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Histone-lysine N-methyltransferase met-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Feb 27, 2013 this sequence version replaced NM_066447.4. COMPLETENESS: incomplete on both ends.

ATGGACCAAC AAGAACCATC GAATAACGTA GATACGAGCA GTATTCTTTC GGATGATGGG 
ATGGAAACAC AGGAACAAAG TTCATTCGTC ACTGCTACAA TTGACCTAAC AGTGGACGAC 
TACGATGAAA CAGAAATACA GGAGATTCTG GATAATGGAA AAGCAGAAGA AGGAACAGAT 
GAAGATTCTG ATTTAGTTGA AGGGATTCTT AACGCTAATT CAGATGTCCA AGCGCTCCTT 
GATGCGCCAT CTGAGCAAGT AGCTCAAGCT CTTAATTCGT TCTTCGGAAA TGAGAGTGAA 
CAAGAAGCTG TTGCAGCACA AAGACGGGTT GATGCGGAGA AGACTGCCAA AGATGAAGCT 
GAACTCAAGC AACAGGAAGA GGCGGAAGAT CTTATTATAG AAGATTCGAT AGTCAAAACT 
GATGAAGAAA AACAAGCAGT TCGAAGACTG AAAATCAACG AATTTTTATC GTGGTTCACA 
AGGCTCCTTC CAGAACAATT TAAAAATTTC GAATTCACAA ATCCGAACTA TCTGACAGAA 
TCTATCAGCG ATTCACCGGT TGTAAATGTC GATAAATGCA AGGAAATTGT CAAATCGTTC 
AAGGAAAGTG AATCACTTGA GGGACTTTCA CAGAAATACG AATTAATTGA TGAAGACGTG 
CTAGTCGCTG CTATTTGTAT TGGCGTTCTC GATACCAACA ACGAAGAAGA TGTCGACTTT 
AATGTTCTAT GTGATGATCG TATCGACGAT TGGAGTATAG AAAAATGTGT CACTTTTCTT 
GATTATCCAA ATACTGGATT GAATTCGAAA AATGGACCGT TGAGATTCAT GCAGTTTACT 
GTCACATCAC CTGCATCAGC AATTCTCATG CTCACTCTGA TTCGATTACG CGAAGAAGGG 
CATCCGTGTC GATTAGATTT TGATTCAAAT CCGACTGATG ATTTACTCTT GAATTTCGAT 
CAAGTGGAAT TTTCTAATAA TATCATTGAT ACGGCAGTCA AATACTGGGA TGATCAGAAG 
GAAAACGGTG CGCAGGATAA AATTGGCAGA GAGCTCAACG ACTTCTTCCA CGAAATCGAG 
TCAACATCAG CAGAATTCAA ACAACATTTT GAGAACGCCG TTGGCAGCCG TAATGAAATA 
ATTCAACTTG TCAACGAGAA AATTCCCGAT TTTGATGGCA CTGAGGCTGC TGTGAATGAG 
AGTTTTACAT CCGATCAACG AACCGAAATT ATCAACTCTC GTGCAATAAT GGAGACATTA 
AAAGCCGAGA TGAAGCTCGC CATCGCCGAA GCTCAGAAAG TTTACGACAC CAAGACTGAC 
TTCGAAAAAT TCTTCGTTTT GACAGTTGGA GATTTCTGTC TGGCTCGCGC CAATCCTTCT 
GACGATGCAG AATTAACATA CGCCATAGTT CAGGATCGTG TGGATGCAAT GACCTATAAG 
GTTAAATTTA TCGACACAAG TCAGATCAGA GAGTGTAACA TCAGAGATTT AGCCATGACT 
ACGCAGGGAA TGTATGACCC GAGTTTGAAT ACATTTGGTG ATGTTGGTCT TCGAGTTGCC 
TGTCGCCAAG TTATTTCCTC GAGCCAATTT GGAAAAAAAA CAATTTGGCT TACCGGTACA 
GCTGCCGGAC GTCGCAGAGC TCATAGATCC GATTTTCTAA TTTTCTTCGA CAACGGAACC 
GATGCATACG TGTCAGCTCC GACAATGCCT GGTGAACCAG GTTATGAAGT TGCTTCTGAA 
AAGAAAAGTG TATTTTCTCT CAAAGAAATG ATTGCGAAGA TGAATGCTGC TCAGATTGCT 
ATTATGGTTG GACAGCCAGT AGGAAAGGAA GGAAATCTGG ATTATTTTTT GACATTTCAT 
TGGATTCGAC AATCTCACAG ATCAGCGTAT ATTCGGGATT TTATGAAAGA ATTTCCGGAA 
TGGCCACTTC TCAAGATGCC AGTTGGAATG CGAATCTGTT TGTACAATTC TCTTGTTGAT 
CGACGTAAGA AAATGGTGAC AGTGATTGGA ACTGATCGAG CTTTTGCTAT TGTGAGACAC 
GAAGCACCGA ATCCATTGGC TCCTGGGAAT AGATGTACAG ACTTTCCGTG CAATGATAGA 
AATCATCAGC ATATTGACGA GAAAATCTAT AGAGGATCTC ATAGATTGGA AGGCGCAGCG 
CACAAGAAGC ACATGATCTC GACAAATAAC AATCTGTCGC AACGCAGAAA AGACCAGCTT 
CAATCACAGT TCGAGCCAAC CGACATGATT CGTTCGATGC CAGAGAGGAA TCACCAACAA 
GTCGTTAAAA AGAAAACGAC GGGCACCAAT CAGAATGTCG CTTCGACAAA TGATGCAAAA 
TCGAAGAGAG AAATTGAAAT AAGAAAGAAA AATCAATTCT TATTTAACAA GATTATTGTT 
CCAATACCCG TCCTAACACC ATTGGAAAAT CTCAAGGCTC ATGCTCAATG TGGTCCAGAT 
TGTCTACAGA AAATGGATGC GGATCCGTAT GAAGCAAGAT TCCATCGAAA TTCACCAATA 
CATACTCCTC TTTTGTGTGG TTGGAGACGA ATTATGTACA CAATGAGTAC TGGAAAGAAG 
CGGGGAGCAG TGAAGAAAAA CATTATTTAC TTTTCTCCAT GCGGAGCCGC TCTTCACCAG 
ATCAGCGACG TCTCTGAATA TATTCATGTC ACCAGAAGTT TATTGACGAT TGATTGTTTT 
TCATTTGATG CACGAATCGA TACTGCCACT TATATTACTG TTGACGATAA ATATTTGAAG 
GTTGCTGATT TTTCGCTTGG AACCGAAGGA ATCCCAATTC CACTAGTGAA CAGCGTGGAT 
AACGATGAGC CTCCATCATT GGAATATTCG AAACGACGAT TCCAATACAA TGATCAAGTG 
GATATATCGA GTGTTAGCCG AGATTTCTGT TCTGGATGCT CTTGTGATGG TGATTGCAGT 
GACGCATCGA AGTGTGAATG CCAACAATTG TCCATTGAAG CAATGAAACG ACTCCCCCAT 
AATTTACAAT TCGACGGACA CGACGAATTA GTTCCTCACT ATCAAAATCG TCTTCTCAGC 
AGTAAGGTTA TCAGTGGACT CTATGAATGC AACGATCAGT GTTCATGCCA TCGAAAGTCT 
TGTTACAACA GAGTTGTTCA GAACAATATC AAGTATCCTA TGCATATCTT CAAAACTGCT 
CAATCCGGAT GGGGAGTCCG AGCTTTGACG GATATTCCTC AAAGTACGTT CATTTGCACG 
TATGTAGGTG CTATACTGAC GGATGATTTG GCTGATGAAC TAAGAAATGC GGATCAATAC 
TTCGCTGATT TGGACTTGAA GGATACCGTG GAGCTGGAAA AGGGTCGCGA AGATCATGAA 
ACTGATTTTG GTTACGGAGG AGACGAGTCA GATTATGATG ACGAAGAAGG AAGTGATGGT 
GACTCCGGTG ATGATGTAAT GAACAAAATG GTGAAACGTC AAGACTCTTC GGAGAGTGGT 
GAAGAAACAA AACGGCTGAC AAGACAGAAA AGAAAGCAAT CTAAAAAATC CGGTAAAGGA 
GGAAGTGTGG AGAAAGATGA CACCACTCCA AGAGATTCAA TGGAAAAGGA TAATATTGAA 
AGTAAAGACG AACCCGTTTT CAATTGGGAT AAGTATTTTG AGCCGTTTCC ATTGTATGTT 
ATAGATGCAA AACAGAGAGG AAATCTTGGA AGATTCTTGA ATCACTCTTG CGATCCGAAT 
GTGCACGTTC AACACGTCAT GTACGATACG CATGATCTTC GTCTTCCATG GGTCGCGTTT 
TTCACACGAA AATACGTGAA AGCCGGCGAT GAGCTAACCT GGGACTATCA ATATACTCAA 
GATCAGACGG CTACCACACA ACTCACATGC CACTGCGGAG CTGAAAACTG CACCGGCCGT 
TTGCTGAAAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498848.4
CDS	1..3915
Translation	MDQQEPSNNVDTSSILSDDGMETQEQSSFVTATIDLTVDDYDETEIQEILDNGKAEEGTDEDSDLVEGILNANSDVQALLDAPSEQVAQALNSFFGNESEQEAVAAQRRVDAEKTAKDEAELKQQEEAEDLIIEDSIVKTDEEKQAVRRLKINEFLSWFTRLLPEQFKNFEFTNPNYLTESISDSPVVNVDKCKEIVKSFKESESLEGLSQKYELIDEDVLVAAICIGVLDTNNEEDVDFNVLCDDRIDDWSIEKCVTFLDYPNTGLNSKNGPLRFMQFTVTSPASAILMLTLIRLREEGHPCRLDFDSNPTDDLLLNFDQVEFSNNIIDTAVKYWDDQKENGAQDKIGRELNDFFHEIESTSAEFKQHFENAVGSRNEIIQLVNEKIPDFDGTEAAVNESFTSDQRTEIINSRAIMETLKAEMKLAIAEAQKVYDTKTDFEKFFVLTVGDFCLARANPSDDAELTYAIVQDRVDAMTYKVKFIDTSQIRECNIRDLAMTTQGMYDPSLNTFGDVGLRVACRQVISSSQFGKKTIWLTGTAAGRRRAHRSDFLIFFDNGTDAYVSAPTMPGEPGYEVASEKKSVFSLKEMIAKMNAAQIAIMVGQPVGKEGNLDYFLTFHWIRQSHRSAYIRDFMKEFPEWPLLKMPVGMRICLYNSLVDRRKKMVTVIGTDRAFAIVRHEAPNPLAPGNRCTDFPCNDRNHQHIDEKIYRGSHRLEGAAHKKHMISTNNNLSQRRKDQLQSQFEPTDMIRSMPERNHQQVVKKKTTGTNQNVASTNDAKSKREIEIRKKNQFLFNKIIVPIPVLTPLENLKAHAQCGPDCLQKMDADPYEARFHRNSPIHTPLLCGWRRIMYTMSTGKKRGAVKKNIIYFSPCGAALHQISDVSEYIHVTRSLLTIDCFSFDARIDTATYITVDDKYLKVADFSLGTEGIPIPLVNSVDNDEPPSLEYSKRRFQYNDQVDISSVSRDFCSGCSCDGDCSDASKCECQQLSIEAMKRLPHNLQFDGHDELVPHYQNRLLSSKVISGLYECNDQCSCHRKSCYNRVVQNNIKYPMHIFKTAQSGWGVRALTDIPQSTFICTYVGAILTDDLADELRNADQYFADLDLKDTVELEKGREDHETDFGYGGDESDYDDEEGSDGDSGDDVMNKMVKRQDSSESGEETKRLTRQKRKQSKKSGKGGSVEKDDTTPRDSMEKDNIESKDEPVFNWDKYFEPFPLYVIDAKQRGNLGRFLNHSCDPNVHVQHVMYDTHDLRLPWVAFFTRKYVKAGDELTWDYQYTQDQTATTQLTCHCGAENCTGRLLKS

Target ORF information:

RefSeq Version	NM_066447.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Histone-lysine N-methyltransferase met-2 (met-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066447.5

ORF Insert Sequence:

ATGGACCAAC AAGAACCATC GAATAACGTA GATACGAGCA GTATTCTTTC GGATGATGGG 
ATGGAAACAC AGGAACAAAG TTCATTCGTC ACTGCTACAA TTGACCTAAC AGTGGACGAC 
TACGATGAAA CAGAAATACA GGAGATTCTG GATAATGGAA AAGCAGAAGA AGGAACAGAT 
GAAGATTCTG ATTTAGTTGA AGGGATTCTT AACGCTAATT CAGATGTCCA AGCGCTCCTT 
GATGCGCCAT CTGAGCAAGT AGCTCAAGCT CTTAATTCGT TCTTCGGAAA TGAGAGTGAA 
CAAGAAGCTG TTGCAGCACA AAGACGGGTT GATGCGGAGA AGACTGCCAA AGATGAAGCT 
GAACTCAAGC AACAGGAAGA GGCGGAAGAT CTTATTATAG AAGATTCGAT AGTCAAAACT 
GATGAAGAAA AACAAGCAGT TCGAAGACTG AAAATCAACG AATTTTTATC GTGGTTCACA 
AGGCTCCTTC CAGAACAATT TAAAAATTTC GAATTCACAA ATCCGAACTA TCTGACAGAA 
TCTATCAGCG ATTCACCGGT TGTAAATGTC GATAAATGCA AGGAAATTGT CAAATCGTTC 
AAGGAAAGTG AATCACTTGA GGGACTTTCA CAGAAATACG AATTAATTGA TGAAGACGTG 
CTAGTCGCTG CTATTTGTAT TGGCGTTCTC GATACCAACA ACGAAGAAGA TGTCGACTTT 
AATGTTCTAT GTGATGATCG TATCGACGAT TGGAGTATAG AAAAATGTGT CACTTTTCTT 
GATTATCCAA ATACTGGATT GAATTCGAAA AATGGACCGT TGAGATTCAT GCAGTTTACT 
GTCACATCAC CTGCATCAGC AATTCTCATG CTCACTCTGA TTCGATTACG CGAAGAAGGG 
CATCCGTGTC GATTAGATTT TGATTCAAAT CCGACTGATG ATTTACTCTT GAATTTCGAT 
CAAGTGGAAT TTTCTAATAA TATCATTGAT ACGGCAGTCA AATACTGGGA TGATCAGAAG 
GAAAACGGTG CGCAGGATAA AATTGGCAGA GAGCTCAACG ACTTCTTCCA CGAAATCGAG 
TCAACATCAG CAGAATTCAA ACAACATTTT GAGAACGCCG TTGGCAGCCG TAATGAAATA 
ATTCAACTTG TCAACGAGAA AATTCCCGAT TTTGATGGCA CTGAGGCTGC TGTGAATGAG 
AGTTTTACAT CCGATCAACG AACCGAAATT ATCAACTCTC GTGCAATAAT GGAGACATTA 
AAAGCCGAGA TGAAGCTCGC CATCGCCGAA GCTCAGAAAG TTTACGACAC CAAGACTGAC 
TTCGAAAAAT TCTTCGTTTT GACAGTTGGA GATTTCTGTC TGGCTCGCGC CAATCCTTCT 
GACGATGCAG AATTAACATA CGCCATAGTT CAGGATCGTG TGGATGCAAT GACCTATAAG 
GTTAAATTTA TCGACACAAG TCAGATCAGA GAGTGTAACA TCAGAGATTT AGCCATGACT 
ACGCAGGGAA TGTATGACCC GAGTTTGAAT ACATTTGGTG ATGTTGGTCT TCGAGTTGCC 
TGTCGCCAAG TTATTTCCTC GAGCCAATTT GGAAAAAAAA CAATTTGGCT TACCGGTACA 
GCTGCCGGAC GTCGCAGAGC TCATAGATCC GATTTTCTAA TTTTCTTCGA CAACGGAACC 
GATGCATACG TGTCAGCTCC GACAATGCCT GGTGAACCAG GTTATGAAGT TGCTTCTGAA 
AAGAAAAGTG TATTTTCTCT CAAAGAAATG ATTGCGAAGA TGAATGCTGC TCAGATTGCT 
ATTATGGTTG GACAGCCAGT AGGAAAGGAA GGAAATCTGG ATTATTTTTT GACATTTCAT 
TGGATTCGAC AATCTCACAG ATCAGCGTAT ATTCGGGATT TTATGAAAGA ATTTCCGGAA 
TGGCCACTTC TCAAGATGCC AGTTGGAATG CGAATCTGTT TGTACAATTC TCTTGTTGAT 
CGACGTAAGA AAATGGTGAC AGTGATTGGA ACTGATCGAG CTTTTGCTAT TGTGAGACAC 
GAAGCACCGA ATCCATTGGC TCCTGGGAAT AGATGTACAG ACTTTCCGTG CAATGATAGA 
AATCATCAGC ATATTGACGA GAAAATCTAT AGAGGATCTC ATAGATTGGA AGGCGCAGCG 
CACAAGAAGC ACATGATCTC GACAAATAAC AATCTGTCGC AACGCAGAAA AGACCAGCTT 
CAATCACAGT TCGAGCCAAC CGACATGATT CGTTCGATGC CAGAGAGGAA TCACCAACAA 
GTCGTTAAAA AGAAAACGAC GGGCACCAAT CAGAATGTCG CTTCGACAAA TGATGCAAAA 
TCGAAGAGAG AAATTGAAAT AAGAAAGAAA AATCAATTCT TATTTAACAA GATTATTGTT 
CCAATACCCG TCCTAACACC ATTGGAAAAT CTCAAGGCTC ATGCTCAATG TGGTCCAGAT 
TGTCTACAGA AAATGGATGC GGATCCGTAT GAAGCAAGAT TCCATCGAAA TTCACCAATA 
CATACTCCTC TTTTGTGTGG TTGGAGACGA ATTATGTACA CAATGAGTAC TGGAAAGAAG 
CGGGGAGCAG TGAAGAAAAA CATTATTTAC TTTTCTCCAT GCGGAGCCGC TCTTCACCAG 
ATCAGCGACG TCTCTGAATA TATTCATGTC ACCAGAAGTT TATTGACGAT TGATTGTTTT 
TCATTTGATG CACGAATCGA TACTGCCACT TATATTACTG TTGACGATAA ATATTTGAAG 
GTTGCTGATT TTTCGCTTGG AACCGAAGGA ATCCCAATTC CACTAGTGAA CAGCGTGGAT 
AACGATGAGC CTCCATCATT GGAATATTCG AAACGACGAT TCCAATACAA TGATCAAGTG 
GATATATCGA GTGTTAGCCG AGATTTCTGT TCTGGATGCT CTTGTGATGG TGATTGCAGT 
GACGCATCGA AGTGTGAATG CCAACAATTG TCCATTGAAG CAATGAAACG ACTCCCCCAT 
AATTTACAAT TCGACGGACA CGACGAATTA GTTCCTCACT ATCAAAATCG TCTTCTCAGC 
AGTAAGGTTA TCAGTGGACT CTATGAATGC AACGATCAGT GTTCATGCCA TCGAAAGTCT 
TGTTACAACA GAGTTGTTCA GAACAATATC AAGTATCCTA TGCATATCTT CAAAACTGCT 
CAATCCGGAT GGGGAGTCCG AGCTTTGACG GATATTCCTC AAAGTACGTT CATTTGCACG 
TATGTAGGTG CTATACTGAC GGATGATTTG GCTGATGAAC TAAGAAATGC GGATCAATAC 
TTCGCTGATT TGGACTTGAA GGATACCGTG GAGCTGGAAA AGGGTCGCGA AGATCATGAA 
ACTGATTTTG GTTACGGAGG AGACGAGTCA GATTATGATG ACGAAGAAGG AAGTGATGGT 
GACTCCGGTG ATGATGTAAT GAACAAAATG GTGAAACGTC AAGACTCTTC GGAGAGTGGT 
GAAGAAACAA AACGGCTGAC AAGACAGAAA AGAAAGCAAT CTAAAAAATC CGGTAAAGGA 
GGAAGTGTGG AGAAAGATGA CACCACTCCA AGAGATTCAA TGGAAAAGGA TAATATTGAA 
AGTAAAGACG AACCCGTTTT CAATTGGGAT AAGTATTTTG AGCCGTTTCC ATTGTATGTT 
ATAGATGCAA AACAGAGAGG AAATCTTGGA AGATTCTTGA ATCACTCTTG CGATCCGAAT 
GTGCACGTTC AACACGTCAT GTACGATACG CATGATCTTC GTCTTCCATG GGTCGCGTTT 
TTCACACGAA AATACGTGAA AGCCGGCGAT GAGCTAACCT GGGACTATCA ATATACTCAA 
GATCAGACGG CTACCACACA ACTCACATGC CACTGCGGAG CTGAAAACTG CACCGGCCGT 
TTGCTGAAAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

met-2 ( NM_066447.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498848.4 Caenorhabditis elegans Histone-lysine N-methyltransferase met-2 (met-2), partial mRNA.

Price & Availability↑

CloneID	OCa340049
Clone ID Related Accession (Same CDS sequence)	NM_066447.5 , NM_066447.6
Accession Version	NM_066447.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Histone-lysine N-methyltransferase met-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066447.5. COMPLETENESS: incomplete on the 5' end.

ATGGACCAAC AAGAACCATC GAATAACGTA GATACGAGCA GTATTCTTTC GGATGATGGG 
ATGGAAACAC AGGAACAAAG TTCATTCGTC ACTGCTACAA TTGACCTAAC AGTGGACGAC 
TACGATGAAA CAGAAATACA GGAGATTCTG GATAATGGAA AAGCAGAAGA AGGAACAGAT 
GAAGATTCTG ATTTAGTTGA AGGGATTCTT AACGCTAATT CAGATGTCCA AGCGCTCCTT 
GATGCGCCAT CTGAGCAAGT AGCTCAAGCT CTTAATTCGT TCTTCGGAAA TGAGAGTGAA 
CAAGAAGCTG TTGCAGCACA AAGACGGGTT GATGCGGAGA AGACTGCCAA AGATGAAGCT 
GAACTCAAGC AACAGGAAGA GGCGGAAGAT CTTATTATAG AAGATTCGAT AGTCAAAACT 
GATGAAGAAA AACAAGCAGT TCGAAGACTG AAAATCAACG AATTTTTATC GTGGTTCACA 
AGGCTCCTTC CAGAACAATT TAAAAATTTC GAATTCACAA ATCCGAACTA TCTGACAGAA 
TCTATCAGCG ATTCACCGGT TGTAAATGTC GATAAATGCA AGGAAATTGT CAAATCGTTC 
AAGGAAAGTG AATCACTTGA GGGACTTTCA CAGAAATACG AATTAATTGA TGAAGACGTG 
CTAGTCGCTG CTATTTGTAT TGGCGTTCTC GATACCAACA ACGAAGAAGA TGTCGACTTT 
AATGTTCTAT GTGATGATCG TATCGACGAT TGGAGTATAG AAAAATGTGT CACTTTTCTT 
GATTATCCAA ATACTGGATT GAATTCGAAA AATGGACCGT TGAGATTCAT GCAGTTTACT 
GTCACATCAC CTGCATCAGC AATTCTCATG CTCACTCTGA TTCGATTACG CGAAGAAGGG 
CATCCGTGTC GATTAGATTT TGATTCAAAT CCGACTGATG ATTTACTCTT GAATTTCGAT 
CAAGTGGAAT TTTCTAATAA TATCATTGAT ACGGCAGTCA AATACTGGGA TGATCAGAAG 
GAAAACGGTG CGCAGGATAA AATTGGCAGA GAGCTCAACG ACTTCTTCCA CGAAATCGAG 
TCAACATCAG CAGAATTCAA ACAACATTTT GAGAACGCCG TTGGCAGCCG TAATGAAATA 
ATTCAACTTG TCAACGAGAA AATTCCCGAT TTTGATGGCA CTGAGGCTGC TGTGAATGAG 
AGTTTTACAT CCGATCAACG AACCGAAATT ATCAACTCTC GTGCAATAAT GGAGACATTA 
AAAGCCGAGA TGAAGCTCGC CATCGCCGAA GCTCAGAAAG TTTACGACAC CAAGACTGAC 
TTCGAAAAAT TCTTCGTTTT GACAGTTGGA GATTTCTGTC TGGCTCGCGC CAATCCTTCT 
GACGATGCAG AATTAACATA CGCCATAGTT CAGGATCGTG TGGATGCAAT GACCTATAAG 
GTTAAATTTA TCGACACAAG TCAGATCAGA GAGTGTAACA TCAGAGATTT AGCCATGACT 
ACGCAGGGAA TGTATGACCC GAGTTTGAAT ACATTTGGTG ATGTTGGTCT TCGAGTTGCC 
TGTCGCCAAG TTATTTCCTC GAGCCAATTT GGAAAAAAAA CAATTTGGCT TACCGGTACA 
GCTGCCGGAC GTCGCAGAGC TCATAGATCC GATTTTCTAA TTTTCTTCGA CAACGGAACC 
GATGCATACG TGTCAGCTCC GACAATGCCT GGTGAACCAG GTTATGAAGT TGCTTCTGAA 
AAGAAAAGTG TATTTTCTCT CAAAGAAATG ATTGCGAAGA TGAATGCTGC TCAGATTGCT 
ATTATGGTTG GACAGCCAGT AGGAAAGGAA GGAAATCTGG ATTATTTTTT GACATTTCAT 
TGGATTCGAC AATCTCACAG ATCAGCGTAT ATTCGGGATT TTATGAAAGA ATTTCCGGAA 
TGGCCACTTC TCAAGATGCC AGTTGGAATG CGAATCTGTT TGTACAATTC TCTTGTTGAT 
CGACGTAAGA AAATGGTGAC AGTGATTGGA ACTGATCGAG CTTTTGCTAT TGTGAGACAC 
GAAGCACCGA ATCCATTGGC TCCTGGGAAT AGATGTACAG ACTTTCCGTG CAATGATAGA 
AATCATCAGC ATATTGACGA GAAAATCTAT AGAGGATCTC ATAGATTGGA AGGCGCAGCG 
CACAAGAAGC ACATGATCTC GACAAATAAC AATCTGTCGC AACGCAGAAA AGACCAGCTT 
CAATCACAGT TCGAGCCAAC CGACATGATT CGTTCGATGC CAGAGAGGAA TCACCAACAA 
GTCGTTAAAA AGAAAACGAC GGGCACCAAT CAGAATGTCG CTTCGACAAA TGATGCAAAA 
TCGAAGAGAG AAATTGAAAT AAGAAAGAAA AATCAATTCT TATTTAACAA GATTATTGTT 
CCAATACCCG TCCTAACACC ATTGGAAAAT CTCAAGGCTC ATGCTCAATG TGGTCCAGAT 
TGTCTACAGA AAATGGATGC GGATCCGTAT GAAGCAAGAT TCCATCGAAA TTCACCAATA 
CATACTCCTC TTTTGTGTGG TTGGAGACGA ATTATGTACA CAATGAGTAC TGGAAAGAAG 
CGGGGAGCAG TGAAGAAAAA CATTATTTAC TTTTCTCCAT GCGGAGCCGC TCTTCACCAG 
ATCAGCGACG TCTCTGAATA TATTCATGTC ACCAGAAGTT TATTGACGAT TGATTGTTTT 
TCATTTGATG CACGAATCGA TACTGCCACT TATATTACTG TTGACGATAA ATATTTGAAG 
GTTGCTGATT TTTCGCTTGG AACCGAAGGA ATCCCAATTC CACTAGTGAA CAGCGTGGAT 
AACGATGAGC CTCCATCATT GGAATATTCG AAACGACGAT TCCAATACAA TGATCAAGTG 
GATATATCGA GTGTTAGCCG AGATTTCTGT TCTGGATGCT CTTGTGATGG TGATTGCAGT 
GACGCATCGA AGTGTGAATG CCAACAATTG TCCATTGAAG CAATGAAACG ACTCCCCCAT 
AATTTACAAT TCGACGGACA CGACGAATTA GTTCCTCACT ATCAAAATCG TCTTCTCAGC 
AGTAAGGTTA TCAGTGGACT CTATGAATGC AACGATCAGT GTTCATGCCA TCGAAAGTCT 
TGTTACAACA GAGTTGTTCA GAACAATATC AAGTATCCTA TGCATATCTT CAAAACTGCT 
CAATCCGGAT GGGGAGTCCG AGCTTTGACG GATATTCCTC AAAGTACGTT CATTTGCACG 
TATGTAGGTG CTATACTGAC GGATGATTTG GCTGATGAAC TAAGAAATGC GGATCAATAC 
TTCGCTGATT TGGACTTGAA GGATACCGTG GAGCTGGAAA AGGGTCGCGA AGATCATGAA 
ACTGATTTTG GTTACGGAGG AGACGAGTCA GATTATGATG ACGAAGAAGG AAGTGATGGT 
GACTCCGGTG ATGATGTAAT GAACAAAATG GTGAAACGTC AAGACTCTTC GGAGAGTGGT 
GAAGAAACAA AACGGCTGAC AAGACAGAAA AGAAAGCAAT CTAAAAAATC CGGTAAAGGA 
GGAAGTGTGG AGAAAGATGA CACCACTCCA AGAGATTCAA TGGAAAAGGA TAATATTGAA 
AGTAAAGACG AACCCGTTTT CAATTGGGAT AAGTATTTTG AGCCGTTTCC ATTGTATGTT 
ATAGATGCAA AACAGAGAGG AAATCTTGGA AGATTCTTGA ATCACTCTTG CGATCCGAAT 
GTGCACGTTC AACACGTCAT GTACGATACG CATGATCTTC GTCTTCCATG GGTCGCGTTT 
TTCACACGAA AATACGTGAA AGCCGGCGAT GAGCTAACCT GGGACTATCA ATATACTCAA 
GATCAGACGG CTACCACACA ACTCACATGC CACTGCGGAG CTGAAAACTG CACCGGCCGT 
TTGCTGAAAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498848.4
CDS	1..3915
Translation	MDQQEPSNNVDTSSILSDDGMETQEQSSFVTATIDLTVDDYDETEIQEILDNGKAEEGTDEDSDLVEGILNANSDVQALLDAPSEQVAQALNSFFGNESEQEAVAAQRRVDAEKTAKDEAELKQQEEAEDLIIEDSIVKTDEEKQAVRRLKINEFLSWFTRLLPEQFKNFEFTNPNYLTESISDSPVVNVDKCKEIVKSFKESESLEGLSQKYELIDEDVLVAAICIGVLDTNNEEDVDFNVLCDDRIDDWSIEKCVTFLDYPNTGLNSKNGPLRFMQFTVTSPASAILMLTLIRLREEGHPCRLDFDSNPTDDLLLNFDQVEFSNNIIDTAVKYWDDQKENGAQDKIGRELNDFFHEIESTSAEFKQHFENAVGSRNEIIQLVNEKIPDFDGTEAAVNESFTSDQRTEIINSRAIMETLKAEMKLAIAEAQKVYDTKTDFEKFFVLTVGDFCLARANPSDDAELTYAIVQDRVDAMTYKVKFIDTSQIRECNIRDLAMTTQGMYDPSLNTFGDVGLRVACRQVISSSQFGKKTIWLTGTAAGRRRAHRSDFLIFFDNGTDAYVSAPTMPGEPGYEVASEKKSVFSLKEMIAKMNAAQIAIMVGQPVGKEGNLDYFLTFHWIRQSHRSAYIRDFMKEFPEWPLLKMPVGMRICLYNSLVDRRKKMVTVIGTDRAFAIVRHEAPNPLAPGNRCTDFPCNDRNHQHIDEKIYRGSHRLEGAAHKKHMISTNNNLSQRRKDQLQSQFEPTDMIRSMPERNHQQVVKKKTTGTNQNVASTNDAKSKREIEIRKKNQFLFNKIIVPIPVLTPLENLKAHAQCGPDCLQKMDADPYEARFHRNSPIHTPLLCGWRRIMYTMSTGKKRGAVKKNIIYFSPCGAALHQISDVSEYIHVTRSLLTIDCFSFDARIDTATYITVDDKYLKVADFSLGTEGIPIPLVNSVDNDEPPSLEYSKRRFQYNDQVDISSVSRDFCSGCSCDGDCSDASKCECQQLSIEAMKRLPHNLQFDGHDELVPHYQNRLLSSKVISGLYECNDQCSCHRKSCYNRVVQNNIKYPMHIFKTAQSGWGVRALTDIPQSTFICTYVGAILTDDLADELRNADQYFADLDLKDTVELEKGREDHETDFGYGGDESDYDDEEGSDGDSGDDVMNKMVKRQDSSESGEETKRLTRQKRKQSKKSGKGGSVEKDDTTPRDSMEKDNIESKDEPVFNWDKYFEPFPLYVIDAKQRGNLGRFLNHSCDPNVHVQHVMYDTHDLRLPWVAFFTRKYVKAGDELTWDYQYTQDQTATTQLTCHCGAENCTGRLLKS

Target ORF information:

RefSeq Version	NM_066447.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Histone-lysine N-methyltransferase met-2 (met-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066447.6

ORF Insert Sequence:

ATGGACCAAC AAGAACCATC GAATAACGTA GATACGAGCA GTATTCTTTC GGATGATGGG 
ATGGAAACAC AGGAACAAAG TTCATTCGTC ACTGCTACAA TTGACCTAAC AGTGGACGAC 
TACGATGAAA CAGAAATACA GGAGATTCTG GATAATGGAA AAGCAGAAGA AGGAACAGAT 
GAAGATTCTG ATTTAGTTGA AGGGATTCTT AACGCTAATT CAGATGTCCA AGCGCTCCTT 
GATGCGCCAT CTGAGCAAGT AGCTCAAGCT CTTAATTCGT TCTTCGGAAA TGAGAGTGAA 
CAAGAAGCTG TTGCAGCACA AAGACGGGTT GATGCGGAGA AGACTGCCAA AGATGAAGCT 
GAACTCAAGC AACAGGAAGA GGCGGAAGAT CTTATTATAG AAGATTCGAT AGTCAAAACT 
GATGAAGAAA AACAAGCAGT TCGAAGACTG AAAATCAACG AATTTTTATC GTGGTTCACA 
AGGCTCCTTC CAGAACAATT TAAAAATTTC GAATTCACAA ATCCGAACTA TCTGACAGAA 
TCTATCAGCG ATTCACCGGT TGTAAATGTC GATAAATGCA AGGAAATTGT CAAATCGTTC 
AAGGAAAGTG AATCACTTGA GGGACTTTCA CAGAAATACG AATTAATTGA TGAAGACGTG 
CTAGTCGCTG CTATTTGTAT TGGCGTTCTC GATACCAACA ACGAAGAAGA TGTCGACTTT 
AATGTTCTAT GTGATGATCG TATCGACGAT TGGAGTATAG AAAAATGTGT CACTTTTCTT 
GATTATCCAA ATACTGGATT GAATTCGAAA AATGGACCGT TGAGATTCAT GCAGTTTACT 
GTCACATCAC CTGCATCAGC AATTCTCATG CTCACTCTGA TTCGATTACG CGAAGAAGGG 
CATCCGTGTC GATTAGATTT TGATTCAAAT CCGACTGATG ATTTACTCTT GAATTTCGAT 
CAAGTGGAAT TTTCTAATAA TATCATTGAT ACGGCAGTCA AATACTGGGA TGATCAGAAG 
GAAAACGGTG CGCAGGATAA AATTGGCAGA GAGCTCAACG ACTTCTTCCA CGAAATCGAG 
TCAACATCAG CAGAATTCAA ACAACATTTT GAGAACGCCG TTGGCAGCCG TAATGAAATA 
ATTCAACTTG TCAACGAGAA AATTCCCGAT TTTGATGGCA CTGAGGCTGC TGTGAATGAG 
AGTTTTACAT CCGATCAACG AACCGAAATT ATCAACTCTC GTGCAATAAT GGAGACATTA 
AAAGCCGAGA TGAAGCTCGC CATCGCCGAA GCTCAGAAAG TTTACGACAC CAAGACTGAC 
TTCGAAAAAT TCTTCGTTTT GACAGTTGGA GATTTCTGTC TGGCTCGCGC CAATCCTTCT 
GACGATGCAG AATTAACATA CGCCATAGTT CAGGATCGTG TGGATGCAAT GACCTATAAG 
GTTAAATTTA TCGACACAAG TCAGATCAGA GAGTGTAACA TCAGAGATTT AGCCATGACT 
ACGCAGGGAA TGTATGACCC GAGTTTGAAT ACATTTGGTG ATGTTGGTCT TCGAGTTGCC 
TGTCGCCAAG TTATTTCCTC GAGCCAATTT GGAAAAAAAA CAATTTGGCT TACCGGTACA 
GCTGCCGGAC GTCGCAGAGC TCATAGATCC GATTTTCTAA TTTTCTTCGA CAACGGAACC 
GATGCATACG TGTCAGCTCC GACAATGCCT GGTGAACCAG GTTATGAAGT TGCTTCTGAA 
AAGAAAAGTG TATTTTCTCT CAAAGAAATG ATTGCGAAGA TGAATGCTGC TCAGATTGCT 
ATTATGGTTG GACAGCCAGT AGGAAAGGAA GGAAATCTGG ATTATTTTTT GACATTTCAT 
TGGATTCGAC AATCTCACAG ATCAGCGTAT ATTCGGGATT TTATGAAAGA ATTTCCGGAA 
TGGCCACTTC TCAAGATGCC AGTTGGAATG CGAATCTGTT TGTACAATTC TCTTGTTGAT 
CGACGTAAGA AAATGGTGAC AGTGATTGGA ACTGATCGAG CTTTTGCTAT TGTGAGACAC 
GAAGCACCGA ATCCATTGGC TCCTGGGAAT AGATGTACAG ACTTTCCGTG CAATGATAGA 
AATCATCAGC ATATTGACGA GAAAATCTAT AGAGGATCTC ATAGATTGGA AGGCGCAGCG 
CACAAGAAGC ACATGATCTC GACAAATAAC AATCTGTCGC AACGCAGAAA AGACCAGCTT 
CAATCACAGT TCGAGCCAAC CGACATGATT CGTTCGATGC CAGAGAGGAA TCACCAACAA 
GTCGTTAAAA AGAAAACGAC GGGCACCAAT CAGAATGTCG CTTCGACAAA TGATGCAAAA 
TCGAAGAGAG AAATTGAAAT AAGAAAGAAA AATCAATTCT TATTTAACAA GATTATTGTT 
CCAATACCCG TCCTAACACC ATTGGAAAAT CTCAAGGCTC ATGCTCAATG TGGTCCAGAT 
TGTCTACAGA AAATGGATGC GGATCCGTAT GAAGCAAGAT TCCATCGAAA TTCACCAATA 
CATACTCCTC TTTTGTGTGG TTGGAGACGA ATTATGTACA CAATGAGTAC TGGAAAGAAG 
CGGGGAGCAG TGAAGAAAAA CATTATTTAC TTTTCTCCAT GCGGAGCCGC TCTTCACCAG 
ATCAGCGACG TCTCTGAATA TATTCATGTC ACCAGAAGTT TATTGACGAT TGATTGTTTT 
TCATTTGATG CACGAATCGA TACTGCCACT TATATTACTG TTGACGATAA ATATTTGAAG 
GTTGCTGATT TTTCGCTTGG AACCGAAGGA ATCCCAATTC CACTAGTGAA CAGCGTGGAT 
AACGATGAGC CTCCATCATT GGAATATTCG AAACGACGAT TCCAATACAA TGATCAAGTG 
GATATATCGA GTGTTAGCCG AGATTTCTGT TCTGGATGCT CTTGTGATGG TGATTGCAGT 
GACGCATCGA AGTGTGAATG CCAACAATTG TCCATTGAAG CAATGAAACG ACTCCCCCAT 
AATTTACAAT TCGACGGACA CGACGAATTA GTTCCTCACT ATCAAAATCG TCTTCTCAGC 
AGTAAGGTTA TCAGTGGACT CTATGAATGC AACGATCAGT GTTCATGCCA TCGAAAGTCT 
TGTTACAACA GAGTTGTTCA GAACAATATC AAGTATCCTA TGCATATCTT CAAAACTGCT 
CAATCCGGAT GGGGAGTCCG AGCTTTGACG GATATTCCTC AAAGTACGTT CATTTGCACG 
TATGTAGGTG CTATACTGAC GGATGATTTG GCTGATGAAC TAAGAAATGC GGATCAATAC 
TTCGCTGATT TGGACTTGAA GGATACCGTG GAGCTGGAAA AGGGTCGCGA AGATCATGAA 
ACTGATTTTG GTTACGGAGG AGACGAGTCA GATTATGATG ACGAAGAAGG AAGTGATGGT 
GACTCCGGTG ATGATGTAAT GAACAAAATG GTGAAACGTC AAGACTCTTC GGAGAGTGGT 
GAAGAAACAA AACGGCTGAC AAGACAGAAA AGAAAGCAAT CTAAAAAATC CGGTAAAGGA 
GGAAGTGTGG AGAAAGATGA CACCACTCCA AGAGATTCAA TGGAAAAGGA TAATATTGAA 
AGTAAAGACG AACCCGTTTT CAATTGGGAT AAGTATTTTG AGCCGTTTCC ATTGTATGTT 
ATAGATGCAA AACAGAGAGG AAATCTTGGA AGATTCTTGA ATCACTCTTG CGATCCGAAT 
GTGCACGTTC AACACGTCAT GTACGATACG CATGATCTTC GTCTTCCATG GGTCGCGTTT 
TTCACACGAA AATACGTGAA AGCCGGCGAT GAGCTAACCT GGGACTATCA ATATACTCAA 
GATCAGACGG CTACCACACA ACTCACATGC CACTGCGGAG CTGAAAACTG CACCGGCCGT 
TTGCTGAAAA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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met-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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