fbf-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	fbf-2
Entrez Gene ID	174017
Full Name	Fem-3 mRNA-binding factor 2
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_062819.5	NP_495220.1	Fem-3 mRNA-binding factor 2
NM_062819.4	NP_495220.1	Fem-3 mRNA-binding factor 2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	fbf-1	NP_495216.1
Caenorhabditis elegans (roundworm)	fbf-2	NP_495220.1
Caenorhabditis elegans (roundworm)	puf-11	NP_741425.1
Caenorhabditis elegans (roundworm)	puf-3	NP_502606.1
Caenorhabditis elegans (roundworm)	puf-6	NP_496773.1
Caenorhabditis elegans (roundworm)	puf-5	NP_495814.1
Caenorhabditis elegans (roundworm)	puf-4	NP_500301.1
Caenorhabditis elegans (roundworm)	puf-7	NP_500820.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that a 10-amino-acid region in germline development defective-3 (GLD-3) is required for fem-3 binding factor (FBF) binding, while a 7-amino-acid loop in FBF between PUF repeats 7 and 8 is necessary for GLD-3 binding.
Title: A protein.protein interaction platform involved in recruitment of GLD-3 to the FBF.fem-3 mRNA complex.

Data show that CPB-1 (cytoplasmic polyadenylation element binding protein homolog-1) alters the affinity of FBF (fem-3 mRNA binding factor) for specific RNA sequences.
Title: Biochemical characterization of the Caenorhabditis elegans FBF.CPB-1 translational regulation complex identifies conserved protein interaction hotspots.

findings show FBF-1 and FBF-2 have different effects on target mRNAs; FBF-2 prevents translation; FBF-2 activity depends on PGL-1; PGL-1 is required to localize FBF-2 to perinuclear P granules and for efficient binding of FBF-2 to its mRNA targets
Title: The P granule component PGL-1 promotes the localization and silencing activity of the PUF protein FBF-2 in germline stem cells.

Data suggest that the FBF-2/GLD-2 protein directed polyadenylation of the endogenous mRNA, compensating for that mRNA's natural deadenylation.
Title: Targeted translational regulation using the PUF protein family scaffold.

The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells.
Title: The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells.

The following fbf-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fbf-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa338830	NM_062819.5 Latest version!	Caenorhabditis elegans Fem-3 mRNA-binding factor 2 (fbf-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OCa338830	NM_062819.4	Caenorhabditis elegans Fem-3 mRNA-binding factor 2 (fbf-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fbf-2 ( NM_062819.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495220.1 Caenorhabditis elegans Fem-3 mRNA-binding factor 2 (fbf-2), mRNA.

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CloneID	OCa338830
Clone ID Related Accession (Same CDS sequence)	NM_062819.5 , NM_062819.4
Accession Version	NM_062819.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Fem-3 mRNA-binding factor 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_062819.4.

ATGGACCAAT CAAAGATGCG CAGAACGAAT CAGTTCAGAA AAACTTCCCA GAAGCCTCCT 
TCTACAGGGA TTGACAGTTA TCCGACTCCC GCACAATCAC CAATGGCGCA GCATGAGACA 
CCTATGTGGG ACTTCAACTC TCTCAACCCG TATTTCTCAA TGCTCAATAT GAACGATGGG 
ATTAATTATG CTCGCCATCA ACAGAATCAC ATTGTTACAT CGCGGCCACC AACCCCGCTA 
ACTGATTTGA TGAGCCTTCG GAGTTTTCAG AGCTTTCCCA ATGTGTTCAT GCCGGTCTCG 
CGTAGCCGAA CTTCATCATT CATTCAGTCT GATACGGATT CCTCGCGTCT CGAGTCGGAT 
GATTTTTCAC AAAATGTCCG ATGTTTCTCT GCGGATATTG ATCGTAGCAA GAGCTATGGT 
TCTTCAAAAC ACTACCATTT GAAATATTCG AGACCCGCTC TGTCGAGGAA CTCTAGATCA 
TTCACAAGAT CAAACAATGT GCTGCCAACC TGGTCCTTGG ATTCCAATGG TGAAATGCGT 
TCAAGATTGT CGCTCTCTGA AGTTTTGGAT AGTGGCGATT TAATGAAGTT TGCCGTTGAC 
AAAACTGGGT GCCAGTTCCT AGAAAAGGCA GTGAAGGGCA GCCTGACATC TTATCAGAAG 
TTCCAATTGT TTGAACAAGT TATTGGGAGA AAAGATGATT TCCTTAAATT GAGCACGAAC 
ATCTTTGGAA ATTATTTGGT ACAAAGCGTT ATTGGAATTT CATTGGCAAC TAATGATGAC 
GGATACACAA AGCGACAAGA AAAGTTGAAG AACTTCATCA GTTCCCAAAT GACAGATATG 
TGCTTGGACA AATTTGCGTG TCGTGTTATC CAATCCAGTC TCCAGAACAT GGATTTATCC 
CTTGCGTGTA AACTTGTTCA AGCTCTACCT CGTGATGCTC GTCTTATCGC AATCTGTGTT 
GATCAAAATG CCAATCACGT CATTCAGAAA GTCGTAGCAG TCATCCCACT GAAGAATTGG 
GAGTTTATTG TGGATTTCGT TGCCACCCCA GAGCACCTCC GTCAGATTTG TTCTGATAAG 
TATGGGTGCC GTGTTGTGCA AACTATTATC GAAAAGCTCA CTGCTGATTC AATGAACGTT 
GATTTAACTT CCGCTGCTCA AAATCTTCGT GAACGAGCGC TCCAACGACT CATGACTTCT 
GTGACTAATC GGTGCCAAGA ATTAGCTACG AACGAGTATG CCAATTACAT AATACAGCAT 
ATTGTATCCA ATGATGATCT GGCCGTTTAT CGGGAATGTA TCATAGAGAA ATGCCTCATG 
CGCAACCTTC TTTCGTTGTC CCAGGAAAAG TTTGCTTCTC ATGTTGTTGA GAAAGCATTC 
CTACATGCAC CATTGGAACT TCTTGCCGAA ATGATGGACG AGATTTTCGA CGGATACATT 
CCACATCCGG ACACGGGAAA AGATGCCCTC GATATCATGA TGTTCCATCA ATTCGGAAAC 
TACGTCGTTC AATGCATGCT GACTATTTGC TGTGATGCTG TGTCTGGAAG ACGGCAAACG 
AAAGAAGGAG GCTATGACCA TGCAATCTCA TTCCAGGATT GGCTGAAAAA GTTGCACTCG 
AGAGTTACGA AAGAAAGGCA TCGCTTGTCA AGGTTCTCAT CCGGCAAAAA GATGATCGAG 
ACGCTTGCCA ATCTCCGTTC AACTCATCCG ATTTATGAAC TCCAATCTTC GGGACACGAT 
TCATTCAAGA CGGATTACTT CAGTACTGCT TCCGAGCACG ATGGTCCGGA GTTGGAGAAG 
AATGGGATCG AGGAAGGAAG CCTGATGCTA GAGCCACGGA GCAATAAGTC TTCAGTCTCT 
GTCAAATTTT CTTCTTCCGG TAGTCACGGC GATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495220.1
CDS	110..2008
Translation	MDQSKMRRTNQFRKTSQKPPSTGIDSYPTPAQSPMAQHETPMWDFNSLNPYFSMLNMNDGINYARHQQNHIVTSRPPTPLTDLMSLRSFQSFPNVFMPVSRSRTSSFIQSDTDSSRLESDDFSQNVRCFSADIDRSKSYGSSKHYHLKYSRPALSRNSRSFTRSNNVLPTWSLDSNGEMRSRLSLSEVLDSGDLMKFAVDKTGCQFLEKAVKGSLTSYQKFQLFEQVIGRKDDFLKLSTNIFGNYLVQSVIGISLATNDDGYTKRQEKLKNFISSQMTDMCLDKFACRVIQSSLQNMDLSLACKLVQALPRDARLIAICVDQNANHVIQKVVAVIPLKNWEFIVDFVATPEHLRQICSDKYGCRVVQTIIEKLTADSMNVDLTSAAQNLRERALQRLMTSVTNRCQELATNEYANYIIQHIVSNDDLAVYRECIIEKCLMRNLLSLSQEKFASHVVEKAFLHAPLELLAEMMDEIFDGYIPHPDTGKDALDIMMFHQFGNYVVQCMLTICCDAVSGRRQTKEGGYDHAISFQDWLKKLHSRVTKERHRLSRFSSGKKMIETLANLRSTHPIYELQSSGHDSFKTDYFSTASEHDGPELEKNGIEEGSLMLEPRSNKSSVSVKFSSSGSHGDD

Target ORF information:

RefSeq Version	NM_062819.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Fem-3 mRNA-binding factor 2 (fbf-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062819.5

ORF Insert Sequence:

ATGGACCAAT CAAAGATGCG CAGAACGAAT CAGTTCAGAA AAACTTCCCA GAAGCCTCCT 
TCTACAGGGA TTGACAGTTA TCCGACTCCC GCACAATCAC CAATGGCGCA GCATGAGACA 
CCTATGTGGG ACTTCAACTC TCTCAACCCG TATTTCTCAA TGCTCAATAT GAACGATGGG 
ATTAATTATG CTCGCCATCA ACAGAATCAC ATTGTTACAT CGCGGCCACC AACCCCGCTA 
ACTGATTTGA TGAGCCTTCG GAGTTTTCAG AGCTTTCCCA ATGTGTTCAT GCCGGTCTCG 
CGTAGCCGAA CTTCATCATT CATTCAGTCT GATACGGATT CCTCGCGTCT CGAGTCGGAT 
GATTTTTCAC AAAATGTCCG ATGTTTCTCT GCGGATATTG ATCGTAGCAA GAGCTATGGT 
TCTTCAAAAC ACTACCATTT GAAATATTCG AGACCCGCTC TGTCGAGGAA CTCTAGATCA 
TTCACAAGAT CAAACAATGT GCTGCCAACC TGGTCCTTGG ATTCCAATGG TGAAATGCGT 
TCAAGATTGT CGCTCTCTGA AGTTTTGGAT AGTGGCGATT TAATGAAGTT TGCCGTTGAC 
AAAACTGGGT GCCAGTTCCT AGAAAAGGCA GTGAAGGGCA GCCTGACATC TTATCAGAAG 
TTCCAATTGT TTGAACAAGT TATTGGGAGA AAAGATGATT TCCTTAAATT GAGCACGAAC 
ATCTTTGGAA ATTATTTGGT ACAAAGCGTT ATTGGAATTT CATTGGCAAC TAATGATGAC 
GGATACACAA AGCGACAAGA AAAGTTGAAG AACTTCATCA GTTCCCAAAT GACAGATATG 
TGCTTGGACA AATTTGCGTG TCGTGTTATC CAATCCAGTC TCCAGAACAT GGATTTATCC 
CTTGCGTGTA AACTTGTTCA AGCTCTACCT CGTGATGCTC GTCTTATCGC AATCTGTGTT 
GATCAAAATG CCAATCACGT CATTCAGAAA GTCGTAGCAG TCATCCCACT GAAGAATTGG 
GAGTTTATTG TGGATTTCGT TGCCACCCCA GAGCACCTCC GTCAGATTTG TTCTGATAAG 
TATGGGTGCC GTGTTGTGCA AACTATTATC GAAAAGCTCA CTGCTGATTC AATGAACGTT 
GATTTAACTT CCGCTGCTCA AAATCTTCGT GAACGAGCGC TCCAACGACT CATGACTTCT 
GTGACTAATC GGTGCCAAGA ATTAGCTACG AACGAGTATG CCAATTACAT AATACAGCAT 
ATTGTATCCA ATGATGATCT GGCCGTTTAT CGGGAATGTA TCATAGAGAA ATGCCTCATG 
CGCAACCTTC TTTCGTTGTC CCAGGAAAAG TTTGCTTCTC ATGTTGTTGA GAAAGCATTC 
CTACATGCAC CATTGGAACT TCTTGCCGAA ATGATGGACG AGATTTTCGA CGGATACATT 
CCACATCCGG ACACGGGAAA AGATGCCCTC GATATCATGA TGTTCCATCA ATTCGGAAAC 
TACGTCGTTC AATGCATGCT GACTATTTGC TGTGATGCTG TGTCTGGAAG ACGGCAAACG 
AAAGAAGGAG GCTATGACCA TGCAATCTCA TTCCAGGATT GGCTGAAAAA GTTGCACTCG 
AGAGTTACGA AAGAAAGGCA TCGCTTGTCA AGGTTCTCAT CCGGCAAAAA GATGATCGAG 
ACGCTTGCCA ATCTCCGTTC AACTCATCCG ATTTATGAAC TCCAATCTTC GGGACACGAT 
TCATTCAAGA CGGATTACTT CAGTACTGCT TCCGAGCACG ATGGTCCGGA GTTGGAGAAG 
AATGGGATCG AGGAAGGAAG CCTGATGCTA GAGCCACGGA GCAATAAGTC TTCAGTCTCT 
GTCAAATTTT CTTCTTCCGG TAGTCACGGC GATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fbf-2 ( NM_062819.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495220.1 Caenorhabditis elegans Fem-3 mRNA-binding factor 2 (fbf-2), partial mRNA.

Price & Availability↑

CloneID	OCa338830
Clone ID Related Accession (Same CDS sequence)	NM_062819.5 , NM_062819.4
Accession Version	NM_062819.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Fem-3 mRNA-binding factor 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_062819.3. COMPLETENESS: incomplete on both ends.

ATGGACCAAT CAAAGATGCG CAGAACGAAT CAGTTCAGAA AAACTTCCCA GAAGCCTCCT 
TCTACAGGGA TTGACAGTTA TCCGACTCCC GCACAATCAC CAATGGCGCA GCATGAGACA 
CCTATGTGGG ACTTCAACTC TCTCAACCCG TATTTCTCAA TGCTCAATAT GAACGATGGG 
ATTAATTATG CTCGCCATCA ACAGAATCAC ATTGTTACAT CGCGGCCACC AACCCCGCTA 
ACTGATTTGA TGAGCCTTCG GAGTTTTCAG AGCTTTCCCA ATGTGTTCAT GCCGGTCTCG 
CGTAGCCGAA CTTCATCATT CATTCAGTCT GATACGGATT CCTCGCGTCT CGAGTCGGAT 
GATTTTTCAC AAAATGTCCG ATGTTTCTCT GCGGATATTG ATCGTAGCAA GAGCTATGGT 
TCTTCAAAAC ACTACCATTT GAAATATTCG AGACCCGCTC TGTCGAGGAA CTCTAGATCA 
TTCACAAGAT CAAACAATGT GCTGCCAACC TGGTCCTTGG ATTCCAATGG TGAAATGCGT 
TCAAGATTGT CGCTCTCTGA AGTTTTGGAT AGTGGCGATT TAATGAAGTT TGCCGTTGAC 
AAAACTGGGT GCCAGTTCCT AGAAAAGGCA GTGAAGGGCA GCCTGACATC TTATCAGAAG 
TTCCAATTGT TTGAACAAGT TATTGGGAGA AAAGATGATT TCCTTAAATT GAGCACGAAC 
ATCTTTGGAA ATTATTTGGT ACAAAGCGTT ATTGGAATTT CATTGGCAAC TAATGATGAC 
GGATACACAA AGCGACAAGA AAAGTTGAAG AACTTCATCA GTTCCCAAAT GACAGATATG 
TGCTTGGACA AATTTGCGTG TCGTGTTATC CAATCCAGTC TCCAGAACAT GGATTTATCC 
CTTGCGTGTA AACTTGTTCA AGCTCTACCT CGTGATGCTC GTCTTATCGC AATCTGTGTT 
GATCAAAATG CCAATCACGT CATTCAGAAA GTCGTAGCAG TCATCCCACT GAAGAATTGG 
GAGTTTATTG TGGATTTCGT TGCCACCCCA GAGCACCTCC GTCAGATTTG TTCTGATAAG 
TATGGGTGCC GTGTTGTGCA AACTATTATC GAAAAGCTCA CTGCTGATTC AATGAACGTT 
GATTTAACTT CCGCTGCTCA AAATCTTCGT GAACGAGCGC TCCAACGACT CATGACTTCT 
GTGACTAATC GGTGCCAAGA ATTAGCTACG AACGAGTATG CCAATTACAT AATACAGCAT 
ATTGTATCCA ATGATGATCT GGCCGTTTAT CGGGAATGTA TCATAGAGAA ATGCCTCATG 
CGCAACCTTC TTTCGTTGTC CCAGGAAAAG TTTGCTTCTC ATGTTGTTGA GAAAGCATTC 
CTACATGCAC CATTGGAACT TCTTGCCGAA ATGATGGACG AGATTTTCGA CGGATACATT 
CCACATCCGG ACACGGGAAA AGATGCCCTC GATATCATGA TGTTCCATCA ATTCGGAAAC 
TACGTCGTTC AATGCATGCT GACTATTTGC TGTGATGCTG TGTCTGGAAG ACGGCAAACG 
AAAGAAGGAG GCTATGACCA TGCAATCTCA TTCCAGGATT GGCTGAAAAA GTTGCACTCG 
AGAGTTACGA AAGAAAGGCA TCGCTTGTCA AGGTTCTCAT CCGGCAAAAA GATGATCGAG 
ACGCTTGCCA ATCTCCGTTC AACTCATCCG ATTTATGAAC TCCAATCTTC GGGACACGAT 
TCATTCAAGA CGGATTACTT CAGTACTGCT TCCGAGCACG ATGGTCCGGA GTTGGAGAAG 
AATGGGATCG AGGAAGGAAG CCTGATGCTA GAGCCACGGA GCAATAAGTC TTCAGTCTCT 
GTCAAATTTT CTTCTTCCGG TAGTCACGGC GATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495220.1
CDS	1..1899
Translation	MDQSKMRRTNQFRKTSQKPPSTGIDSYPTPAQSPMAQHETPMWDFNSLNPYFSMLNMNDGINYARHQQNHIVTSRPPTPLTDLMSLRSFQSFPNVFMPVSRSRTSSFIQSDTDSSRLESDDFSQNVRCFSADIDRSKSYGSSKHYHLKYSRPALSRNSRSFTRSNNVLPTWSLDSNGEMRSRLSLSEVLDSGDLMKFAVDKTGCQFLEKAVKGSLTSYQKFQLFEQVIGRKDDFLKLSTNIFGNYLVQSVIGISLATNDDGYTKRQEKLKNFISSQMTDMCLDKFACRVIQSSLQNMDLSLACKLVQALPRDARLIAICVDQNANHVIQKVVAVIPLKNWEFIVDFVATPEHLRQICSDKYGCRVVQTIIEKLTADSMNVDLTSAAQNLRERALQRLMTSVTNRCQELATNEYANYIIQHIVSNDDLAVYRECIIEKCLMRNLLSLSQEKFASHVVEKAFLHAPLELLAEMMDEIFDGYIPHPDTGKDALDIMMFHQFGNYVVQCMLTICCDAVSGRRQTKEGGYDHAISFQDWLKKLHSRVTKERHRLSRFSSGKKMIETLANLRSTHPIYELQSSGHDSFKTDYFSTASEHDGPELEKNGIEEGSLMLEPRSNKSSVSVKFSSSGSHGDD

Target ORF information:

RefSeq Version	NM_062819.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Fem-3 mRNA-binding factor 2 (fbf-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_062819.4

ORF Insert Sequence:

ATGGACCAAT CAAAGATGCG CAGAACGAAT CAGTTCAGAA AAACTTCCCA GAAGCCTCCT 
TCTACAGGGA TTGACAGTTA TCCGACTCCC GCACAATCAC CAATGGCGCA GCATGAGACA 
CCTATGTGGG ACTTCAACTC TCTCAACCCG TATTTCTCAA TGCTCAATAT GAACGATGGG 
ATTAATTATG CTCGCCATCA ACAGAATCAC ATTGTTACAT CGCGGCCACC AACCCCGCTA 
ACTGATTTGA TGAGCCTTCG GAGTTTTCAG AGCTTTCCCA ATGTGTTCAT GCCGGTCTCG 
CGTAGCCGAA CTTCATCATT CATTCAGTCT GATACGGATT CCTCGCGTCT CGAGTCGGAT 
GATTTTTCAC AAAATGTCCG ATGTTTCTCT GCGGATATTG ATCGTAGCAA GAGCTATGGT 
TCTTCAAAAC ACTACCATTT GAAATATTCG AGACCCGCTC TGTCGAGGAA CTCTAGATCA 
TTCACAAGAT CAAACAATGT GCTGCCAACC TGGTCCTTGG ATTCCAATGG TGAAATGCGT 
TCAAGATTGT CGCTCTCTGA AGTTTTGGAT AGTGGCGATT TAATGAAGTT TGCCGTTGAC 
AAAACTGGGT GCCAGTTCCT AGAAAAGGCA GTGAAGGGCA GCCTGACATC TTATCAGAAG 
TTCCAATTGT TTGAACAAGT TATTGGGAGA AAAGATGATT TCCTTAAATT GAGCACGAAC 
ATCTTTGGAA ATTATTTGGT ACAAAGCGTT ATTGGAATTT CATTGGCAAC TAATGATGAC 
GGATACACAA AGCGACAAGA AAAGTTGAAG AACTTCATCA GTTCCCAAAT GACAGATATG 
TGCTTGGACA AATTTGCGTG TCGTGTTATC CAATCCAGTC TCCAGAACAT GGATTTATCC 
CTTGCGTGTA AACTTGTTCA AGCTCTACCT CGTGATGCTC GTCTTATCGC AATCTGTGTT 
GATCAAAATG CCAATCACGT CATTCAGAAA GTCGTAGCAG TCATCCCACT GAAGAATTGG 
GAGTTTATTG TGGATTTCGT TGCCACCCCA GAGCACCTCC GTCAGATTTG TTCTGATAAG 
TATGGGTGCC GTGTTGTGCA AACTATTATC GAAAAGCTCA CTGCTGATTC AATGAACGTT 
GATTTAACTT CCGCTGCTCA AAATCTTCGT GAACGAGCGC TCCAACGACT CATGACTTCT 
GTGACTAATC GGTGCCAAGA ATTAGCTACG AACGAGTATG CCAATTACAT AATACAGCAT 
ATTGTATCCA ATGATGATCT GGCCGTTTAT CGGGAATGTA TCATAGAGAA ATGCCTCATG 
CGCAACCTTC TTTCGTTGTC CCAGGAAAAG TTTGCTTCTC ATGTTGTTGA GAAAGCATTC 
CTACATGCAC CATTGGAACT TCTTGCCGAA ATGATGGACG AGATTTTCGA CGGATACATT 
CCACATCCGG ACACGGGAAA AGATGCCCTC GATATCATGA TGTTCCATCA ATTCGGAAAC 
TACGTCGTTC AATGCATGCT GACTATTTGC TGTGATGCTG TGTCTGGAAG ACGGCAAACG 
AAAGAAGGAG GCTATGACCA TGCAATCTCA TTCCAGGATT GGCTGAAAAA GTTGCACTCG 
AGAGTTACGA AAGAAAGGCA TCGCTTGTCA AGGTTCTCAT CCGGCAAAAA GATGATCGAG 
ACGCTTGCCA ATCTCCGTTC AACTCATCCG ATTTATGAAC TCCAATCTTC GGGACACGAT 
TCATTCAAGA CGGATTACTT CAGTACTGCT TCCGAGCACG ATGGTCCGGA GTTGGAGAAG 
AATGGGATCG AGGAAGGAAG CCTGATGCTA GAGCCACGGA GCAATAAGTC TTCAGTCTCT 
GTCAAATTTT CTTCTTCCGG TAGTCACGGC GATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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fbf-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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