RAD50 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD50
Entrez Gene ID	855471
Full Name	MRX complex DNA-binding subunit
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183088.1	NP_014149.1	MRX complex DNA-binding subunit

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rad50	XP_001713090.2
Kluyveromyces lactis	KLLA0C02915g	XP_452327.1
Eremothecium gossypii	AGOS_AFR637W	NP_986184.2
Saccharomyces cerevisiae (baker's yeast)	RAD50	NP_014149.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Xrs2 dependent and independent functions of the Mre11-Rad50 complex orchestrating the cellular response to double-strand DNA breaks have been described.
Title: Xrs2 Dependent and Independent Functions of the Mre11-Rad50 Complex.

The Saccharomyces cerevisiae Mre11-Rad50-Xrs2 complex promotes trinucleotide repeat expansions independently of homologous recombination.
Title: The Saccharomyces cerevisiae Mre11-Rad50-Xrs2 complex promotes trinucleotide repeat expansions independently of homologous recombination.

Mre11 recruits conjugated SUMO moieties to facilitate the assembly and function of the Mre11-Rad50-Xrs2 complex.
Title: S. cerevisiae Mre11 recruits conjugated SUMO moieties to facilitate the assembly and function of the Mre11-Rad50-Xrs2 complex.

Data suggest that the major function of Sae2 in response to DNA double-strand breaks (DSBs) is to actively remove Mre11-Rad50-Xrs2 from break ends and prevent Rad53 in response to DSBs.
Title: Sae2 promotes DNA damage resistance by removing the Mre11-Rad50-Xrs2 complex from DNA and attenuating Rad53 signaling.

Data demonstrate that the dimerization state of the Rad50 hook domain exerts diverse influences on Mre11 complex function.
Title: Interdependence of the rad50 hook and globular domain functions.

The following RAD50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04399	NM_001183088.1 Latest version!	Saccharomyces cerevisiae S288C MRX complex DNA-binding subunit (RAD50), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD50 ( NM_001183088.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014149.1 Saccharomyces cerevisiae S288C MRX complex DNA-binding subunit (RAD50), partial mRNA.

Price & Availability↑

CloneID	OSi04399
Clone ID Related Accession (Same CDS sequence)	NM_001183088.1
Accession Version	NM_001183088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	MRX complex DNA-binding subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGCGCTA TCTATAAATT ATCTATTCAG GGCATACGGT CTTTTGACTC CAATGATAGG 
GAAACTATTG AATTTGGCAA GCCTCTGACT TTAATAGTCG GCATGAATGG TTCAGGTAAA 
ACGACTATCA TCGAATGTTT AAAGTACGCT ACCACAGGTG ATCTACCGCC CAACAGCAAG 
GGAGGAGTAT TCATTCATGA CCCGAAGATA ACTGGTGAAA AGGACATTAG AGCTCAGGTC 
AAACTGGCGT TTACGAGTGC CAATGGACTC AATATGATTG TCACCAGAAA TATTCAGTTG 
CTAATGAAAA AGACTACTAC TACATTTAAG ACTTTAGAAG GCCAGTTGGT CGCTATAAAT 
AATAGTGGTG ACCGCAGTAC TTTGTCCACC CGGTCTCTGG AATTAGACGC ACAAGTACCC 
CTGTATCTGG GAGTTCCAAA AGCGATCTTG GAATACGTGA TATTCTGTCA CCAAGAAGAC 
AGCCTGTGGC CACTGAGTGA ACCATCCAAT CTTAAAAAGA AGTTTGATGA AATTTTCCAA 
GCGATGAAAT TTACAAAGGC TCTAGACAAT TTAAAATCCA TAAAGAAAGA CATGTCCGTG 
GATATCAAGT TATTAAAGCA ATCAGTAGAA CACTTAAAGT TGGATAAAGA CAGATCGAAA 
GCAATGAAGC TCAATATCCA CCAATTGCAA ACTAAGATAG ATCAGTATAA TGAAGAAGTG 
TCTGAAATTG AATCTCAATT GAATGAAATC ACTGAGAAAT CCGATAAACT GTTTAAATCT 
AATCAAGATT TCCAAAAAAT ACTATCCAAA GTAGAGAACT TGAAAAATAC AAAACTCTCA 
ATTAGTGATC AAGTGAAAAG ATTGTCAAAC TCAATTGATA TTCTCGATTT ATCGAAGCCA 
GATTTGCAAA ACTTATTGGC AAACTTCTCT AAAGTCTTGA TGGATAAGAA TAATCAGTTG 
AGAGATTTGG AAACCGATAT TTCAAGTTTG AAAGACCGTC AGTCATCCTT GCAGAGCCTT 
TCTAATTCCC TCATCAGGCG GCAGGGTGAG TTAGAAGCTG GTAAGGAGAC ATACGAAAAG 
AACAGAAATC ACCTTTCATC GCTAAAAGAA GCCTTCCAAC ATAAGTTCCA AGGCCTTTCA 
AATATTGAAA ACAGTGATAT GGCTCAAGTA AATCACGAAA TGTCACAGTT CAAGGCTTTC 
ATCTCTCAGG ATTTAACTGA CACTATAGAT CAATTTGCAA AGGATATTCA ACTAAAGGAA 
ACAAATTTAT CCGACTTGAT AAAATCAATA ACCGTCGATT CACAGAATTT GGAATATAAT 
AAAAAGGACA GATCTAAATT AATCCATGAC TCTGAAGAGC TTGCTGAGAA ATTGAAATCA 
TTTAAAAGTT TGTCTACTCA AGATAGCCTC AATCATGAAC TGGAAAACTT AAAAACATAC 
AAAGAAAAGC TGCAATCTTG GGAAAGTGAG AATATTATAC CAAAATTGAA CCAGAAAATA 
GAAGAGAAAA ATAATGAGAT GATTATCTTA GAGAATCAAA TTGAGAAATT TCAAGATCGC 
ATAATGAAAA CGAATCAACA AGCGGACTTA TACGCCAAAC TAGGACTAAT AAAGAAGTCC 
ATTAATACTA AATTAGACGA GTTACAAAAG ATTACGGAAA AATTACAGAA TGATTCTAGA 
ATAAGGCAAG TATTTCCCCT CACCCAGGAA TTTCAAAGAG CTGATTTAGA AATGGATTTT 
CAGAAATTAT TCATCAATAT GCAAAAGAAT ATAGCGATCA ACAACAAAAA GATGCACGAA 
TTGGATAGAA GATATACAAA CGCTTTGTAC AATTTGAACA CCATTGAAAA GGATTTACAG 
GATAATCAGA AGTCGAAAGA AAAAGTGATA CAACTACTAA GCGAAAACTT ACCAGAGGAT 
TGTACTATTG ATGAATATAA TGATGTCCTG GAAGAAACGG AGCTCTCCTA TAAGACTGCG 
CTGGAAAACT TGAAAATGCA TCAAACTACT TTAGAATTTA ATAGAAAAGC ATTAGAGATC 
GCTGAACGTG ACAGCTGCTG TTATTTGTGT TCTAGAAAGT TTGAAAATGA ATCGTTTAAA 
AGTAAGTTGT TACAAGAACT AAAAACAAAA ACAGACGCAA ATTTTGAAAA AACTTTGAAA 
GACACTGTCC AAAACGAAAA GGAATATCTT CATAGTTTAA GGCTACTGGA GAAACATATT 
ATTACACTAA ATTCTATAAA TGAAAAGATT GATAATTCAC AGAAGTGCCT TGAAAAAGCT 
AAGGAGGAAA CAAAAACTTC CAAATCAAAA CTAGATGAAT TGGAAGTAGA TTCCACAAAA 
TTGAAAGATG AGAAAGAGTT AGCTGAGTCT GAAATTCGTC CTTTGATTGA GAAGTTTACT 
TACTTAGAAA AGGAACTTAA GGATCTTGAA AACAGTTCTA AAACGATATC TGAGGAGCTA 
TCGATTTATA ATACCAGTGA AGATGGTATT CAAACTGTTG ATGAATTAAG GGACCAACAA 
AGGAAGATGA ATGATTCTTT ACGTGAACTG AGGAAAACCA TTTCAGATTT GCAAATGGAA 
AAAGATGAAA AGGTGAGAGA AAATTCAAGA ATGATTAACT TGATTAAAGA AAAGGAATTA 
ACAGTTTCTG AAATTGAATC ATCTTTAACA CAGAAACAAA ATATTGATGA TTCTATAAGG 
TCAAAAAGGG AAAACATCAA CGATATCGAT TCTAGAGTAA AGGAGTTAGA AGCACGTATT 
ATTTCATTAA AAAACAAAAA GGATGAAGCG CAAAGTGTTC TAGACAAAGT AAAAAATGAA 
CGTGATATTC AAGTACGCAA CAAACAAAAA ACCGTTGCAG ATATTAATCG GTTAATAGAT 
AGATTTCAGA CCATTTATAA CGAAGTGGTT GATTTTGAAG CTAAGGGCTT CGATGAATTG 
CAAACAACAA TAAAAGAACT AGAATTGAAC AAAGCTCAAA TGCTGGAACT AAAGGAGCAG 
CTTGATCTCA AGTCAAATGA AGTTAACGAA GAAAAGAGAA AACTTGCTGA CTCAAATAAT 
GAGGAGAAAA ACTTGAAGCA GAACTTAGAA CTAATTGAAT TGAAATCACA ACTTCAGCAC 
ATTGAATCTG AGATAAGTAG ATTAGACGTT CAAAATGCAG AAGCTGAAAG GGACAAATAT 
CAAGAGGAAT CACTAAGACT AAGAACAAGA TTTGAAAAGC TGAGCTCTGA AAACGCAGGT 
AAATTAGGTG AAATGAAACA ATTACAGAAT CAAATAGATT CATTGACTCA TCAACTGCGG 
ACTGATTACA AAGATATCGA GAAAAATTAC CATAAAGAAT GGGTTGAATT ACAGACAAGA 
TCCTTTGTTA CTGACGACAT AGATGTCTAT TCAAAAGCAT TAGATAGTGC TATTATGAAA 
TATCATGGAT TGAAAATGCA AGATATAAAC AGGATAATTG ATGAATTATG GAAGCGTACG 
TATAGTGGTA CTGATATAGA CACCATCAAA ATTAGATCAG ATGAAGTTAG TAGTACAGTT 
AAGGGAAAAT CATACAACTA TCGTGTTGTA ATGTACAAGC AGGATGTTGA ATTGGATATG 
AGAGGACGAT GTTCCGCGGG TCAAAAAGTT CTTGCATCGA TTATTATCAG GCTAGCTCTA 
TCTGAAACAT TTGGCGCAAA TTGTGGCGTA ATTGCACTAG ACGAACCTAC CACCAATTTA 
GATGAAGAAA ACATCGAAAG TTTAGCAAAA TCTTTACACA ATATAATTAA TATGAGAAGA 
CATCAAAAGA ATTTCCAGCT AATCGTGATC ACTCATGATG AGAAATTTTT GGGTCATATG 
AATGCAGCGG CATTTACTGA TCACTTCTTC AAAGTCAAGA GAGATGATAG GCAAAAATCA 
CAAATTGAGT GGGTCGATAT TAACAGAGTC ACTTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014149.1
CDS	1..3939
Translation	MSAIYKLSIQGIRSFDSNDRETIEFGKPLTLIVGMNGSGKTTIIECLKYATTGDLPPNSKGGVFIHDPKITGEKDIRAQVKLAFTSANGLNMIVTRNIQLLMKKTTTTFKTLEGQLVAINNSGDRSTLSTRSLELDAQVPLYLGVPKAILEYVIFCHQEDSLWPLSEPSNLKKKFDEIFQAMKFTKALDNLKSIKKDMSVDIKLLKQSVEHLKLDKDRSKAMKLNIHQLQTKIDQYNEEVSEIESQLNEITEKSDKLFKSNQDFQKILSKVENLKNTKLSISDQVKRLSNSIDILDLSKPDLQNLLANFSKVLMDKNNQLRDLETDISSLKDRQSSLQSLSNSLIRRQGELEAGKETYEKNRNHLSSLKEAFQHKFQGLSNIENSDMAQVNHEMSQFKAFISQDLTDTIDQFAKDIQLKETNLSDLIKSITVDSQNLEYNKKDRSKLIHDSEELAEKLKSFKSLSTQDSLNHELENLKTYKEKLQSWESENIIPKLNQKIEEKNNEMIILENQIEKFQDRIMKTNQQADLYAKLGLIKKSINTKLDELQKITEKLQNDSRIRQVFPLTQEFQRADLEMDFQKLFINMQKNIAINNKKMHELDRRYTNALYNLNTIEKDLQDNQKSKEKVIQLLSENLPEDCTIDEYNDVLEETELSYKTALENLKMHQTTLEFNRKALEIAERDSCCYLCSRKFENESFKSKLLQELKTKTDANFEKTLKDTVQNEKEYLHSLRLLEKHIITLNSINEKIDNSQKCLEKAKEETKTSKSKLDELEVDSTKLKDEKELAESEIRPLIEKFTYLEKELKDLENSSKTISEELSIYNTSEDGIQTVDELRDQQRKMNDSLRELRKTISDLQMEKDEKVRENSRMINLIKEKELTVSEIESSLTQKQNIDDSIRSKRENINDIDSRVKELEARIISLKNKKDEAQSVLDKVKNERDIQVRNKQKTVADINRLIDRFQTIYNEVVDFEAKGFDELQTTIKELELNKAQMLELKEQLDLKSNEVNEEKRKLADSNNEEKNLKQNLELIELKSQLQHIESEISRLDVQNAEAERDKYQEESLRLRTRFEKLSSENAGKLGEMKQLQNQIDSLTHQLRTDYKDIEKNYHKEWVELQTRSFVTDDIDVYSKALDSAIMKYHGLKMQDINRIIDELWKRTYSGTDIDTIKIRSDEVSSTVKGKSYNYRVVMYKQDVELDMRGRCSAGQKVLASIIIRLALSETFGANCGVIALDEPTTNLDEENIESLAKSLHNIINMRRHQKNFQLIVITHDEKFLGHMNAAAFTDHFFKVKRDDRQKSQIEWVDINRVTY

Target ORF information:

RefSeq Version	NM_001183088.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C MRX complex DNA-binding subunit (RAD50), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183088.1

ORF Insert Sequence:

ATGAGCGCTA TCTATAAATT ATCTATTCAG GGCATACGGT CTTTTGACTC CAATGATAGG 
GAAACTATTG AATTTGGCAA GCCTCTGACT TTAATAGTCG GCATGAATGG TTCAGGTAAA 
ACGACTATCA TCGAATGTTT AAAGTACGCT ACCACAGGTG ATCTACCGCC CAACAGCAAG 
GGAGGAGTAT TCATTCATGA CCCGAAGATA ACTGGTGAAA AGGACATTAG AGCTCAGGTC 
AAACTGGCGT TTACGAGTGC CAATGGACTC AATATGATTG TCACCAGAAA TATTCAGTTG 
CTAATGAAAA AGACTACTAC TACATTTAAG ACTTTAGAAG GCCAGTTGGT CGCTATAAAT 
AATAGTGGTG ACCGCAGTAC TTTGTCCACC CGGTCTCTGG AATTAGACGC ACAAGTACCC 
CTGTATCTGG GAGTTCCAAA AGCGATCTTG GAATACGTGA TATTCTGTCA CCAAGAAGAC 
AGCCTGTGGC CACTGAGTGA ACCATCCAAT CTTAAAAAGA AGTTTGATGA AATTTTCCAA 
GCGATGAAAT TTACAAAGGC TCTAGACAAT TTAAAATCCA TAAAGAAAGA CATGTCCGTG 
GATATCAAGT TATTAAAGCA ATCAGTAGAA CACTTAAAGT TGGATAAAGA CAGATCGAAA 
GCAATGAAGC TCAATATCCA CCAATTGCAA ACTAAGATAG ATCAGTATAA TGAAGAAGTG 
TCTGAAATTG AATCTCAATT GAATGAAATC ACTGAGAAAT CCGATAAACT GTTTAAATCT 
AATCAAGATT TCCAAAAAAT ACTATCCAAA GTAGAGAACT TGAAAAATAC AAAACTCTCA 
ATTAGTGATC AAGTGAAAAG ATTGTCAAAC TCAATTGATA TTCTCGATTT ATCGAAGCCA 
GATTTGCAAA ACTTATTGGC AAACTTCTCT AAAGTCTTGA TGGATAAGAA TAATCAGTTG 
AGAGATTTGG AAACCGATAT TTCAAGTTTG AAAGACCGTC AGTCATCCTT GCAGAGCCTT 
TCTAATTCCC TCATCAGGCG GCAGGGTGAG TTAGAAGCTG GTAAGGAGAC ATACGAAAAG 
AACAGAAATC ACCTTTCATC GCTAAAAGAA GCCTTCCAAC ATAAGTTCCA AGGCCTTTCA 
AATATTGAAA ACAGTGATAT GGCTCAAGTA AATCACGAAA TGTCACAGTT CAAGGCTTTC 
ATCTCTCAGG ATTTAACTGA CACTATAGAT CAATTTGCAA AGGATATTCA ACTAAAGGAA 
ACAAATTTAT CCGACTTGAT AAAATCAATA ACCGTCGATT CACAGAATTT GGAATATAAT 
AAAAAGGACA GATCTAAATT AATCCATGAC TCTGAAGAGC TTGCTGAGAA ATTGAAATCA 
TTTAAAAGTT TGTCTACTCA AGATAGCCTC AATCATGAAC TGGAAAACTT AAAAACATAC 
AAAGAAAAGC TGCAATCTTG GGAAAGTGAG AATATTATAC CAAAATTGAA CCAGAAAATA 
GAAGAGAAAA ATAATGAGAT GATTATCTTA GAGAATCAAA TTGAGAAATT TCAAGATCGC 
ATAATGAAAA CGAATCAACA AGCGGACTTA TACGCCAAAC TAGGACTAAT AAAGAAGTCC 
ATTAATACTA AATTAGACGA GTTACAAAAG ATTACGGAAA AATTACAGAA TGATTCTAGA 
ATAAGGCAAG TATTTCCCCT CACCCAGGAA TTTCAAAGAG CTGATTTAGA AATGGATTTT 
CAGAAATTAT TCATCAATAT GCAAAAGAAT ATAGCGATCA ACAACAAAAA GATGCACGAA 
TTGGATAGAA GATATACAAA CGCTTTGTAC AATTTGAACA CCATTGAAAA GGATTTACAG 
GATAATCAGA AGTCGAAAGA AAAAGTGATA CAACTACTAA GCGAAAACTT ACCAGAGGAT 
TGTACTATTG ATGAATATAA TGATGTCCTG GAAGAAACGG AGCTCTCCTA TAAGACTGCG 
CTGGAAAACT TGAAAATGCA TCAAACTACT TTAGAATTTA ATAGAAAAGC ATTAGAGATC 
GCTGAACGTG ACAGCTGCTG TTATTTGTGT TCTAGAAAGT TTGAAAATGA ATCGTTTAAA 
AGTAAGTTGT TACAAGAACT AAAAACAAAA ACAGACGCAA ATTTTGAAAA AACTTTGAAA 
GACACTGTCC AAAACGAAAA GGAATATCTT CATAGTTTAA GGCTACTGGA GAAACATATT 
ATTACACTAA ATTCTATAAA TGAAAAGATT GATAATTCAC AGAAGTGCCT TGAAAAAGCT 
AAGGAGGAAA CAAAAACTTC CAAATCAAAA CTAGATGAAT TGGAAGTAGA TTCCACAAAA 
TTGAAAGATG AGAAAGAGTT AGCTGAGTCT GAAATTCGTC CTTTGATTGA GAAGTTTACT 
TACTTAGAAA AGGAACTTAA GGATCTTGAA AACAGTTCTA AAACGATATC TGAGGAGCTA 
TCGATTTATA ATACCAGTGA AGATGGTATT CAAACTGTTG ATGAATTAAG GGACCAACAA 
AGGAAGATGA ATGATTCTTT ACGTGAACTG AGGAAAACCA TTTCAGATTT GCAAATGGAA 
AAAGATGAAA AGGTGAGAGA AAATTCAAGA ATGATTAACT TGATTAAAGA AAAGGAATTA 
ACAGTTTCTG AAATTGAATC ATCTTTAACA CAGAAACAAA ATATTGATGA TTCTATAAGG 
TCAAAAAGGG AAAACATCAA CGATATCGAT TCTAGAGTAA AGGAGTTAGA AGCACGTATT 
ATTTCATTAA AAAACAAAAA GGATGAAGCG CAAAGTGTTC TAGACAAAGT AAAAAATGAA 
CGTGATATTC AAGTACGCAA CAAACAAAAA ACCGTTGCAG ATATTAATCG GTTAATAGAT 
AGATTTCAGA CCATTTATAA CGAAGTGGTT GATTTTGAAG CTAAGGGCTT CGATGAATTG 
CAAACAACAA TAAAAGAACT AGAATTGAAC AAAGCTCAAA TGCTGGAACT AAAGGAGCAG 
CTTGATCTCA AGTCAAATGA AGTTAACGAA GAAAAGAGAA AACTTGCTGA CTCAAATAAT 
GAGGAGAAAA ACTTGAAGCA GAACTTAGAA CTAATTGAAT TGAAATCACA ACTTCAGCAC 
ATTGAATCTG AGATAAGTAG ATTAGACGTT CAAAATGCAG AAGCTGAAAG GGACAAATAT 
CAAGAGGAAT CACTAAGACT AAGAACAAGA TTTGAAAAGC TGAGCTCTGA AAACGCAGGT 
AAATTAGGTG AAATGAAACA ATTACAGAAT CAAATAGATT CATTGACTCA TCAACTGCGG 
ACTGATTACA AAGATATCGA GAAAAATTAC CATAAAGAAT GGGTTGAATT ACAGACAAGA 
TCCTTTGTTA CTGACGACAT AGATGTCTAT TCAAAAGCAT TAGATAGTGC TATTATGAAA 
TATCATGGAT TGAAAATGCA AGATATAAAC AGGATAATTG ATGAATTATG GAAGCGTACG 
TATAGTGGTA CTGATATAGA CACCATCAAA ATTAGATCAG ATGAAGTTAG TAGTACAGTT 
AAGGGAAAAT CATACAACTA TCGTGTTGTA ATGTACAAGC AGGATGTTGA ATTGGATATG 
AGAGGACGAT GTTCCGCGGG TCAAAAAGTT CTTGCATCGA TTATTATCAG GCTAGCTCTA 
TCTGAAACAT TTGGCGCAAA TTGTGGCGTA ATTGCACTAG ACGAACCTAC CACCAATTTA 
GATGAAGAAA ACATCGAAAG TTTAGCAAAA TCTTTACACA ATATAATTAA TATGAGAAGA 
CATCAAAAGA ATTTCCAGCT AATCGTGATC ACTCATGATG AGAAATTTTT GGGTCATATG 
AATGCAGCGG CATTTACTGA TCACTTCTTC AAAGTCAAGA GAGATGATAG GCAAAAATCA 
CAAATTGAGT GGGTCGATAT TAACAGAGTC ACTTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RAD50 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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