rad50 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	rad50
Entrez Gene ID	2541635
Full Name	DNA repair protein Rad50
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001713038.3	XP_001713090.2	DNA repair protein Rad50
NM_001356129.1	NP_001342968.1	DNA repair protein Rad50

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rad50	XP_001713090.2
Kluyveromyces lactis	KLLA0C02915g	XP_452327.1
Eremothecium gossypii	AGOS_AFR637W	NP_986184.2
Saccharomyces cerevisiae (baker's yeast)	RAD50	NP_014149.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a mre11 mutant defective in endonucleolytic activity, the same mutant lacking Ctp1, or the triple mutant also lacking the putative hairpin nuclease Pso2 showed wild-type levels of repair. Thus, MRN (Mre11-Rad50-Nbs1)may act to recruit the hairpin opening activity that allows subsequent repair
Title: Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in

Mre11-Rad50-Nbs1 participates in checkpoint-dependent replication slowing through negative regulation of recombination.
Title: The fission yeast Rad32(Mre11)-Rad50-Nbs1 complex acts both upstream and downstream of checkpoint signaling in the S-phase DNA damage checkpoint.

Rad32 nuclease has the catalytic site for Rec12-oligonucleotide generation and is activated by Ctp1, which plays an additional role in meiotic recombination.
Title: Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes.

Study finds that in the presence of the MRN (Rad32-Rad50-Nbs1) complex efficient recombination requires Ctp1, the ortholog of the nuclease Sae2, but not the nuclease activity of MRN.
Title: Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination.

The Mre11-Rad50-Nbs1 S-phase checkpoint role is separate from its Ctp1- and resection-dependent role in double-strand break repair.
Title: The role of MRN in the S-phase DNA damage checkpoint is independent of its Ctp1-dependent roles in double-strand break repair and checkpoint signaling.

The following rad50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102006	NM_001356129.1 Latest version!	Schizosaccharomyces pombe DNA repair protein Rad50 (rad50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OSj02887	XM_001713038.3 Latest version!	Schizosaccharomyces pombe 972h- DNA repair protein Rad50 (rad50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rad50 ( NM_001356129.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342968.1 Schizosaccharomyces pombe DNA repair protein Rad50 (rad50), mRNA.

Price & Availability↑

CloneID	OSc102006
Clone ID Related Accession (Same CDS sequence)	NM_001356129.1
Accession Version	NM_001356129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	DNA repair protein Rad50
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGTCGTGCA TTGACAGAAT GTCCATCATG GGCATACGGT CGTTTGACAA TCGTTCACGA 
GAAAGCATTC AATTTTTCTC CCCATTAACG CTAATAGTAG GACAAAATGG GTCTGGAAAG 
ACTACAATAA TTGAATGCTT AAAGTACGCA ACAACAGGAA TACTCCCCCC AAATACAAAA 
GGTGGAGCCT TCATTCATGA TCCAAAAATA TGCGGTGAAA AAGAGGTCCT TGCTCAGGTC 
AAATTGGCGT TTCGAAATAC GAACCAAGTT AAGATGATCT GTACCAGATC ATTACAATTA 
TCTGTTAAAA AAACTACTCG ACAACAGAAA ACCTTGGACG GACAATTGCT AATATTGAAA 
GACAATGAAA GAACTACAAT TAGCAATCGT TGTGCCGAAT TAGATTCTCA AGTACCGCTC 
AGCTTGGGTG TGAGTAAAGC TTTATTAGAC TATGTCATTT TTTGTCATCA GGAAGAGAGC 
TTCTGGCCAT TAAGTGAGCC TGCTAATTTA AAAAAAAGGT TTGATGAGAT ATTTGAATCA 
TTGCGATATG CAAAGGCTCT GGATCAAATA AAGGGTTTAA AGCGCGACCA GGAAACTCAA 
GTGAAGGTCG ATCAAGCGAC ATTGACACAT TACCGCTCAG ACAAAGAAAG AGCCGAGAAA 
ATTGAATTGA GGGTACATGA ATCTTTGAAG CGTATATCCT GTATCCGATC TAAAGTCGAA 
GAGCTTGACC AAGAGATAAC TGAAACGGCT AGGCTACAAG ATGAGCTTTT CAAATCCACT 
GAAGAATATG AACAACAAAT GATTACAATC AGACATTTGG AAAGTCAAAG TGACATTATT 
AATACTACTA TCAACGACCT TAAAAGTCAA ATGACTATAA CTGACGAAAG TTCTGAAGAT 
TTGGAGAAAC TTCATTCGAA TTTTGCTGAA AAGGTTAAAG AGGAACAGGA ACTTTACAAA 
TCTTTAGAGA AGAAGCGTTC TGATTTAGAA AGTCTCCTCA AATCAAGAAG GGAACTCCTT 
GAAAAACTCA CTGGAGATTT GGGTAAAATC CAAGGAGAGA TAGAAAGCTT GGAGAAATTG 
AAGGTAAAAA AATCGACTAT GATTAACGAA ATTGTTCATC GCTACAATAT TAATGAAATA 
AATGAAGAAG GGATCATGAC AGAGGTGTCT AAATACGCTA GCCTTGTAAA TAAAAATTAT 
GAGATTTCCA GTGGTAAACT AAAAGAGCGA CAGGTGGCCG TGAGAGCACG CATCGAAGGA 
ATTAAAGCTC ACGAGATGTT CCTTAATAAT CGTGTTTCGG AAATCAACTC TTCACTTGAA 
AAACAACTCA CAACTCAAAA AGAATTGCGC TCACGCTTTG AAATCCTTTT TCCTGTAAAG 
TTACAGCGAG AAGATTTTAC TAAAGATGTT GAAAAATCGG ATTTGTGGAT TAAATCATTG 
CGTCAAGAGT ATGAAAGTAA AAATTTGCTG GAGTTACTTG ACAAACATCA AACAGCTCTG 
TCTTCAGTTG AAAACCGTTT AGATGAAATT TCAGAAATCG TCGATTCATA CCATAAATAC 
TCTGGAGTTC GTACCAAGTT ACAAGTTTTC GAAGAGAATA AAACCAATAA ATCTGCTATA 
CTGGCTAACC AACTGATGAC ATTGAAAAGT TCGTTTTCAG AAGTTATGTC TTACGAATTA 
AAGGATGATG ATAATTACAA TGAAGAATTG GATAAACTAG TCGAAGATGT ACGTAAAAAG 
TTACAAGAAA AAGAAGAGGC CGAATCATCA CTTCGGTCCG TCAGGGAAAG ACTTGAAATT 
AGAATTTCGT TATCTGTACA AAGTATTAAT GATTTAACCG AGAATAAGAA GATAAAAACT 
AAAACTTTGA AATCTTATAG TGGGACCTTC GCTTCTATGA TATCTGAAAT TAAAGCTTTG 
GAAAGTGAAA TAGAAGAAAA TAGGAAAACC CTTCACTCAT TGCAATTTGG ATCTACATTT 
TATGAGAAAG CTATAGAAAT ATGTGTTGAC CAACATGCTT GTCAACTATG TCAAAGAAGC 
CTCGATAAAG AAGAGGAAAA GCTCTTTGTG GAACACTGTC ACTCCATGAT TGACGTTATA 
CCATCCAAAT CTGCTGAAGT TTATAGTCAC CTTGAAACCC TTACTAAAAC TTTTAAAAAT 
CTTTCTGAAG CTAAACCGAT TTTCGATGAG ATTGAACTGC TTGATAAAAG GCTTTCAGAG 
ACGAAAACTG AACTTTCAGA TTTGCAAGGT GATCTACAAG GTTTGGATAT ACGTAAGGAC 
GAAATTCAAT CAGAATTGGA TACCTTGTAT TTAAGAAGAG CAAACTTAGA GAAATTACAA 
TTGTTGGTTA AAGACATTTC TAATTTAGAG GAAGAAATTC GTACAATTGA CAGAGAAACT 
GAAGTTTTAC GTATTGAACT TCCTTCTTCT ATTGCTCATC ATAATTTAGA CGAAATTTAT 
GCTGAGAGAG AAAAATTACT CGAGAAGAGG GGATACTTAA GGAAACAAAT AGAAAGAACT 
AAATTGGAGG AAACTTCTTT TAAAAAGAAA ATAGACGATG CTGTCTTAGC CAATAACGAG 
CAGAAACTAA AACTTACCAA GTTGAATTTT CAGGTCAATG AATTGGAACA ACTCGAAAAA 
GATATTAACA AATCCTCCGA AGACTGTGAC CTTCAAAAGA AAAAGCTTTT GGAAGTTAGC 
TCTAAACAAG GTTCTCAGGC ACCCTTTTTA AACGAATTAG AGTCAGAGTA TGAAAAATTA 
GAAGCAGATA TTCAAGAAAT GGCTCAAAAA AGTCGGACAG AAATCCTTGA AGCTAATGAA 
TACTTGCATC AACTTAATGA ATGGAACTCT GAATTGAGGA TTGACGTTTC CACAAAGTTT 
AAATGCATAA AAGAGAAGAA AAGTAATATT GGAGAAGAAG TTAGGATTAT TGCTTCTAAA 
ATTGAAAGTA CTGATGATAA TTTAAGAAAG TTACAGGAGC GCTTAGCGGA TTTGCGCACG 
AGGGAAAGGA ACGCATCTGA TAATCTACGC CTTCGCGCTT TAATGAGACA ATTAGAAGAA 
GCCGTGACGC AAAAGAATTA TTTGTTATCC CAGCAATCAC ATGATGATCG GGAATCATTT 
CGCGAACGAA TGCAAATTTT AAAGTCAAAA TACGGGGCAC TCAACGCTGA GCGCGCAGGC 
TTATTAGGAG AATGTAAGCA ATTGGAGAAC TCCATTACTA AAGACAAGGA AGAACTTAAT 
ATGGAGTTTA AAGATGCGGA CGAAAGATTT CGACGACAGC TTATAAAAAC AAAGACTACG 
GGTAAAGCAA ATGAGGATTT GGGCAAGTAT GCGAAAGCTC TTGATGTCGC TATCATGCAA 
CTACATTCAA TGAAAATGAA TGAGATCAAT CGAATAGTAG ACGAACTTTG GAAACAAACC 
TACTGTGGAA CTGACATTGA TACCATTTTA ATTCGCTCTG ATAGTGAAGG TAAAGGGAAT 
AGGACTTACA ATTATAGAGT CTGTATGGTT AAAGGTGATG CCGAGCTAGA TATGCGAGGC 
CGCTGCAGTG CTGGTCAAAA GGTTTTAGCG TGTATTATTA TAAGGTTGGC TTTGGCAGAA 
TGTCTAGGTG TAAACTGTGG AATTTTGGCT CTGGATGAGC CAACTACAAA TCTCGATGAA 
GAGAATATAT GTAGTTTGGC TAAGAATTTA TCTCGGATTG TCGAATTTAG AAGGAAACAA 
GCAAACTTCC AACTCATTGT TATTACGCAT GATGAACAAT TTATCCGGTT AGTTAATTCA 
GATGCTTATT GTAGCTATTA TTATAGGGTG AAACGGGACA CGAATCAGAA GAGCATGATT 
GTTAAAGAAC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342968.1
CDS	168..4025
Translation	MSCIDRMSIMGIRSFDNRSRESIQFFSPLTLIVGQNGSGKTTIIECLKYATTGILPPNTKGGAFIHDPKICGEKEVLAQVKLAFRNTNQVKMICTRSLQLSVKKTTRQQKTLDGQLLILKDNERTTISNRCAELDSQVPLSLGVSKALLDYVIFCHQEESFWPLSEPANLKKRFDEIFESLRYAKALDQIKGLKRDQETQVKVDQATLTHYRSDKERAEKIELRVHESLKRISCIRSKVEELDQEITETARLQDELFKSTEEYEQQMITIRHLESQSDIINTTINDLKSQMTITDESSEDLEKLHSNFAEKVKEEQELYKSLEKKRSDLESLLKSRRELLEKLTGDLGKIQGEIESLEKLKVKKSTMINEIVHRYNINEINEEGIMTEVSKYASLVNKNYEISSGKLKERQVAVRARIEGIKAHEMFLNNRVSEINSSLEKQLTTQKELRSRFEILFPVKLQREDFTKDVEKSDLWIKSLRQEYESKNLLELLDKHQTALSSVENRLDEISEIVDSYHKYSGVRTKLQVFEENKTNKSAILANQLMTLKSSFSEVMSYELKDDDNYNEELDKLVEDVRKKLQEKEEAESSLRSVRERLEIRISLSVQSINDLTENKKIKTKTLKSYSGTFASMISEIKALESEIEENRKTLHSLQFGSTFYEKAIEICVDQHACQLCQRSLDKEEEKLFVEHCHSMIDVIPSKSAEVYSHLETLTKTFKNLSEAKPIFDEIELLDKRLSETKTELSDLQGDLQGLDIRKDEIQSELDTLYLRRANLEKLQLLVKDISNLEEEIRTIDRETEVLRIELPSSIAHHNLDEIYAEREKLLEKRGYLRKQIERTKLEETSFKKKIDDAVLANNEQKLKLTKLNFQVNELEQLEKDINKSSEDCDLQKKKLLEVSSKQGSQAPFLNELESEYEKLEADIQEMAQKSRTEILEANEYLHQLNEWNSELRIDVSTKFKCIKEKKSNIGEEVRIIASKIESTDDNLRKLQERLADLRTRERNASDNLRLRALMRQLEEAVTQKNYLLSQQSHDDRESFRERMQILKSKYGALNAERAGLLGECKQLENSITKDKEELNMEFKDADERFRRQLIKTKTTGKANEDLGKYAKALDVAIMQLHSMKMNEINRIVDELWKQTYCGTDIDTILIRSDSEGKGNRTYNYRVCMVKGDAELDMRGRCSAGQKVLACIIIRLALAECLGVNCGILALDEPTTNLDEENICSLAKNLSRIVEFRRKQANFQLIVITHDEQFIRLVNSDAYCSYYYRVKRDTNQKSMIVKEPL

Target ORF information:

RefSeq Version	NM_001356129.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe DNA repair protein Rad50 (rad50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356129.1

ORF Insert Sequence:

ATGTCGTGCA TTGACAGAAT GTCCATCATG GGCATACGGT CGTTTGACAA TCGTTCACGA 
GAAAGCATTC AATTTTTCTC CCCATTAACG CTAATAGTAG GACAAAATGG GTCTGGAAAG 
ACTACAATAA TTGAATGCTT AAAGTACGCA ACAACAGGAA TACTCCCCCC AAATACAAAA 
GGTGGAGCCT TCATTCATGA TCCAAAAATA TGCGGTGAAA AAGAGGTCCT TGCTCAGGTC 
AAATTGGCGT TTCGAAATAC GAACCAAGTT AAGATGATCT GTACCAGATC ATTACAATTA 
TCTGTTAAAA AAACTACTCG ACAACAGAAA ACCTTGGACG GACAATTGCT AATATTGAAA 
GACAATGAAA GAACTACAAT TAGCAATCGT TGTGCCGAAT TAGATTCTCA AGTACCGCTC 
AGCTTGGGTG TGAGTAAAGC TTTATTAGAC TATGTCATTT TTTGTCATCA GGAAGAGAGC 
TTCTGGCCAT TAAGTGAGCC TGCTAATTTA AAAAAAAGGT TTGATGAGAT ATTTGAATCA 
TTGCGATATG CAAAGGCTCT GGATCAAATA AAGGGTTTAA AGCGCGACCA GGAAACTCAA 
GTGAAGGTCG ATCAAGCGAC ATTGACACAT TACCGCTCAG ACAAAGAAAG AGCCGAGAAA 
ATTGAATTGA GGGTACATGA ATCTTTGAAG CGTATATCCT GTATCCGATC TAAAGTCGAA 
GAGCTTGACC AAGAGATAAC TGAAACGGCT AGGCTACAAG ATGAGCTTTT CAAATCCACT 
GAAGAATATG AACAACAAAT GATTACAATC AGACATTTGG AAAGTCAAAG TGACATTATT 
AATACTACTA TCAACGACCT TAAAAGTCAA ATGACTATAA CTGACGAAAG TTCTGAAGAT 
TTGGAGAAAC TTCATTCGAA TTTTGCTGAA AAGGTTAAAG AGGAACAGGA ACTTTACAAA 
TCTTTAGAGA AGAAGCGTTC TGATTTAGAA AGTCTCCTCA AATCAAGAAG GGAACTCCTT 
GAAAAACTCA CTGGAGATTT GGGTAAAATC CAAGGAGAGA TAGAAAGCTT GGAGAAATTG 
AAGGTAAAAA AATCGACTAT GATTAACGAA ATTGTTCATC GCTACAATAT TAATGAAATA 
AATGAAGAAG GGATCATGAC AGAGGTGTCT AAATACGCTA GCCTTGTAAA TAAAAATTAT 
GAGATTTCCA GTGGTAAACT AAAAGAGCGA CAGGTGGCCG TGAGAGCACG CATCGAAGGA 
ATTAAAGCTC ACGAGATGTT CCTTAATAAT CGTGTTTCGG AAATCAACTC TTCACTTGAA 
AAACAACTCA CAACTCAAAA AGAATTGCGC TCACGCTTTG AAATCCTTTT TCCTGTAAAG 
TTACAGCGAG AAGATTTTAC TAAAGATGTT GAAAAATCGG ATTTGTGGAT TAAATCATTG 
CGTCAAGAGT ATGAAAGTAA AAATTTGCTG GAGTTACTTG ACAAACATCA AACAGCTCTG 
TCTTCAGTTG AAAACCGTTT AGATGAAATT TCAGAAATCG TCGATTCATA CCATAAATAC 
TCTGGAGTTC GTACCAAGTT ACAAGTTTTC GAAGAGAATA AAACCAATAA ATCTGCTATA 
CTGGCTAACC AACTGATGAC ATTGAAAAGT TCGTTTTCAG AAGTTATGTC TTACGAATTA 
AAGGATGATG ATAATTACAA TGAAGAATTG GATAAACTAG TCGAAGATGT ACGTAAAAAG 
TTACAAGAAA AAGAAGAGGC CGAATCATCA CTTCGGTCCG TCAGGGAAAG ACTTGAAATT 
AGAATTTCGT TATCTGTACA AAGTATTAAT GATTTAACCG AGAATAAGAA GATAAAAACT 
AAAACTTTGA AATCTTATAG TGGGACCTTC GCTTCTATGA TATCTGAAAT TAAAGCTTTG 
GAAAGTGAAA TAGAAGAAAA TAGGAAAACC CTTCACTCAT TGCAATTTGG ATCTACATTT 
TATGAGAAAG CTATAGAAAT ATGTGTTGAC CAACATGCTT GTCAACTATG TCAAAGAAGC 
CTCGATAAAG AAGAGGAAAA GCTCTTTGTG GAACACTGTC ACTCCATGAT TGACGTTATA 
CCATCCAAAT CTGCTGAAGT TTATAGTCAC CTTGAAACCC TTACTAAAAC TTTTAAAAAT 
CTTTCTGAAG CTAAACCGAT TTTCGATGAG ATTGAACTGC TTGATAAAAG GCTTTCAGAG 
ACGAAAACTG AACTTTCAGA TTTGCAAGGT GATCTACAAG GTTTGGATAT ACGTAAGGAC 
GAAATTCAAT CAGAATTGGA TACCTTGTAT TTAAGAAGAG CAAACTTAGA GAAATTACAA 
TTGTTGGTTA AAGACATTTC TAATTTAGAG GAAGAAATTC GTACAATTGA CAGAGAAACT 
GAAGTTTTAC GTATTGAACT TCCTTCTTCT ATTGCTCATC ATAATTTAGA CGAAATTTAT 
GCTGAGAGAG AAAAATTACT CGAGAAGAGG GGATACTTAA GGAAACAAAT AGAAAGAACT 
AAATTGGAGG AAACTTCTTT TAAAAAGAAA ATAGACGATG CTGTCTTAGC CAATAACGAG 
CAGAAACTAA AACTTACCAA GTTGAATTTT CAGGTCAATG AATTGGAACA ACTCGAAAAA 
GATATTAACA AATCCTCCGA AGACTGTGAC CTTCAAAAGA AAAAGCTTTT GGAAGTTAGC 
TCTAAACAAG GTTCTCAGGC ACCCTTTTTA AACGAATTAG AGTCAGAGTA TGAAAAATTA 
GAAGCAGATA TTCAAGAAAT GGCTCAAAAA AGTCGGACAG AAATCCTTGA AGCTAATGAA 
TACTTGCATC AACTTAATGA ATGGAACTCT GAATTGAGGA TTGACGTTTC CACAAAGTTT 
AAATGCATAA AAGAGAAGAA AAGTAATATT GGAGAAGAAG TTAGGATTAT TGCTTCTAAA 
ATTGAAAGTA CTGATGATAA TTTAAGAAAG TTACAGGAGC GCTTAGCGGA TTTGCGCACG 
AGGGAAAGGA ACGCATCTGA TAATCTACGC CTTCGCGCTT TAATGAGACA ATTAGAAGAA 
GCCGTGACGC AAAAGAATTA TTTGTTATCC CAGCAATCAC ATGATGATCG GGAATCATTT 
CGCGAACGAA TGCAAATTTT AAAGTCAAAA TACGGGGCAC TCAACGCTGA GCGCGCAGGC 
TTATTAGGAG AATGTAAGCA ATTGGAGAAC TCCATTACTA AAGACAAGGA AGAACTTAAT 
ATGGAGTTTA AAGATGCGGA CGAAAGATTT CGACGACAGC TTATAAAAAC AAAGACTACG 
GGTAAAGCAA ATGAGGATTT GGGCAAGTAT GCGAAAGCTC TTGATGTCGC TATCATGCAA 
CTACATTCAA TGAAAATGAA TGAGATCAAT CGAATAGTAG ACGAACTTTG GAAACAAACC 
TACTGTGGAA CTGACATTGA TACCATTTTA ATTCGCTCTG ATAGTGAAGG TAAAGGGAAT 
AGGACTTACA ATTATAGAGT CTGTATGGTT AAAGGTGATG CCGAGCTAGA TATGCGAGGC 
CGCTGCAGTG CTGGTCAAAA GGTTTTAGCG TGTATTATTA TAAGGTTGGC TTTGGCAGAA 
TGTCTAGGTG TAAACTGTGG AATTTTGGCT CTGGATGAGC CAACTACAAA TCTCGATGAA 
GAGAATATAT GTAGTTTGGC TAAGAATTTA TCTCGGATTG TCGAATTTAG AAGGAAACAA 
GCAAACTTCC AACTCATTGT TATTACGCAT GATGAACAAT TTATCCGGTT AGTTAATTCA 
GATGCTTATT GTAGCTATTA TTATAGGGTG AAACGGGACA CGAATCAGAA GAGCATGATT 
GTTAAAGAAC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad50 ( XM_001713038.3 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> XP_001713090.2 Schizosaccharomyces pombe 972h- DNA repair protein Rad50 (rad50), mRNA.

Price & Availability↑

CloneID	OSj02887
Clone ID Related Accession (Same CDS sequence)	XM_001713038.3
Accession Version	XM_001713038.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	DNA repair protein Rad50
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001713038.2. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGTCGTGCA TTGACAGAAT GTCCATCATG GGCATACGGT CGTTTGACAA TCGTTCACGA 
GAAAGCATTC AATTTTTCTC CCCATTAACG CTAATAGTAG GACAAAATGG GTCTGGAAAG 
ACTACAATAA TTGAATGCTT AAAGTACGCA ACAACAGGAA TACTCCCCCC AAATACAAAA 
GGTGGAGCCT TCATTCATGA TCCAAAAATA TGCGGTGAAA AAGAGGTCCT TGCTCAGGTC 
AAATTGGCGT TTCGAAATAC GAACCAAGTT AAGATGATCT GTACCAGATC ATTACAATTA 
TCTGTTAAAA AAACTACTCG ACAACAGAAA ACCTTGGACG GACAATTGCT AATATTGAAA 
GACAATGAAA GAACTACAAT TAGCAATCGT TGTGCCGAAT TAGATTCTCA AGTACCGCTC 
AGCTTGGGTG TGAGTAAAGC TTTATTAGAC TATGTCATTT TTTGTCATCA GGAAGAGAGC 
TTCTGGCCAT TAAGTGAGCC TGCTAATTTA AAAAAAAGGT TTGATGAGAT ATTTGAATCA 
TTGCGATATG CAAAGGCTCT GGATCAAATA AAGGGTTTAA AGCGCGACCA GGAAACTCAA 
GTGAAGGTCG ATCAAGCGAC ATTGACACAT TACCGCTCAG ACAAAGAAAG AGCCGAGAAA 
ATTGAATTGA GGGTACATGA ATCTTTGAAG CGTATATCCT GTATCCGATC TAAAGTCGAA 
GAGCTTGACC AAGAGATAAC TGAAACGGCT AGGCTACAAG ATGAGCTTTT CAAATCCACT 
GAAGAATATG AACAACAAAT GATTACAATC AGACATTTGG AAAGTCAAAG TGACATTATT 
AATACTACTA TCAACGACCT TAAAAGTCAA ATGACTATAA CTGACGAAAG TTCTGAAGAT 
TTGGAGAAAC TTCATTCGAA TTTTGCTGAA AAGGTTAAAG AGGAACAGGA ACTTTACAAA 
TCTTTAGAGA AGAAGCGTTC TGATTTAGAA AGTCTCCTCA AATCAAGAAG GGAACTCCTT 
GAAAAACTCA CTGGAGATTT GGGTAAAATC CAAGGAGAGA TAGAAAGCTT GGAGAAATTG 
AAGGTAAAAA AATCGACTAT GATTAACGAA ATTGTTCATC GCTACAATAT TAATGAAATA 
AATGAAGAAG GGATCATGAC AGAGGTGTCT AAATACGCTA GCCTTGTAAA TAAAAATTAT 
GAGATTTCCA GTGGTAAACT AAAAGAGCGA CAGGTGGCCG TGAGAGCACG CATCGAAGGA 
ATTAAAGCTC ACGAGATGTT CCTTAATAAT CGTGTTTCGG AAATCAACTC TTCACTTGAA 
AAACAACTCA CAACTCAAAA AGAATTGCGC TCACGCTTTG AAATCCTTTT TCCTGTAAAG 
TTACAGCGAG AAGATTTTAC TAAAGATGTT GAAAAATCGG ATTTGTGGAT TAAATCATTG 
CGTCAAGAGT ATGAAAGTAA AAATTTGCTG GAGTTACTTG ACAAACATCA AACAGCTCTG 
TCTTCAGTTG AAAACCGTTT AGATGAAATT TCAGAAATCG TCGATTCATA CCATAAATAC 
TCTGGAGTTC GTACCAAGTT ACAAGTTTTC GAAGAGAATA AAACCAATAA ATCTGCTATA 
CTGGCTAACC AACTGATGAC ATTGAAAAGT TCGTTTTCAG AAGTTATGTC TTACGAATTA 
AAGGATGATG ATAATTACAA TGAAGAATTG GATAAACTAG TCGAAGATGT ACGTAAAAAG 
TTACAAGAAA AAGAAGAGGC CGAATCATCA CTTCGGTCCG TCAGGGAAAG ACTTGAAATT 
AGAATTTCGT TATCTGTACA AAGTATTAAT GATTTAACCG AGAATAAGAA GATAAAAACT 
AAAACTTTGA AATCTTATAG TGGGACCTTC GCTTCTATGA TATCTGAAAT TAAAGCTTTG 
GAAAGTGAAA TAGAAGAAAA TAGGAAAACC CTTCACTCAT TGCAATTTGG ATCTACATTT 
TATGAGAAAG CTATAGAAAT ATGTGTTGAC CAACATGCTT GTCAACTATG TCAAAGAAGC 
CTCGATAAAG AAGAGGAAAA GCTCTTTGTG GAACACTGTC ACTCCATGAT TGACGTTATA 
CCATCCAAAT CTGCTGAAGT TTATAGTCAC CTTGAAACCC TTACTAAAAC TTTTAAAAAT 
CTTTCTGAAG CTAAACCGAT TTTCGATGAG ATTGAACTGC TTGATAAAAG GCTTTCAGAG 
ACGAAAACTG AACTTTCAGA TTTGCAAGGT GATCTACAAG GTTTGGATAT ACGTAAGGAC 
GAAATTCAAT CAGAATTGGA TACCTTGTAT TTAAGAAGAG CAAACTTAGA GAAATTACAA 
TTGTTGGTTA AAGACATTTC TAATTTAGAG GAAGAAATTC GTACAATTGA CAGAGAAACT 
GAAGTTTTAC GTATTGAACT TCCTTCTTCT ATTGCTCATC ATAATTTAGA CGAAATTTAT 
GCTGAGAGAG AAAAATTACT CGAGAAGAGG GGATACTTAA GGAAACAAAT AGAAAGAACT 
AAATTGGAGG AAACTTCTTT TAAAAAGAAA ATAGACGATG CTGTCTTAGC CAATAACGAG 
CAGAAACTAA AACTTACCAA GTTGAATTTT CAGGTCAATG AATTGGAACA ACTCGAAAAA 
GATATTAACA AATCCTCCGA AGACTGTGAC CTTCAAAAGA AAAAGCTTTT GGAAGTTAGC 
TCTAAACAAG GTTCTCAGGC ACCCTTTTTA AACGAATTAG AGTCAGAGTA TGAAAAATTA 
GAAGCAGATA TTCAAGAAAT GGCTCAAAAA AGTCGGACAG AAATCCTTGA AGCTAATGAA 
TACTTGCATC AACTTAATGA ATGGAACTCT GAATTGAGGA TTGACGTTTC CACAAAGTTT 
AAATGCATAA AAGAGAAGAA AAGTAATATT GGAGAAGAAG TTAGGATTAT TGCTTCTAAA 
ATTGAAAGTA CTGATGATAA TTTAAGAAAG TTACAGGAGC GCTTAGCGGA TTTGCGCACG 
AGGGAAAGGA ACGCATCTGA TAATCTACGC CTTCGCGCTT TAATGAGACA ATTAGAAGAA 
GCCGTGACGC AAAAGAATTA TTTGTTATCC CAGCAATCAC ATGATGATCG GGAATCATTT 
CGCGAACGAA TGCAAATTTT AAAGTCAAAA TACGGGGCAC TCAACGCTGA GCGCGCAGGC 
TTATTAGGAG AATGTAAGCA ATTGGAGAAC TCCATTACTA AAGACAAGGA AGAACTTAAT 
ATGGAGTTTA AAGATGCGGA CGAAAGATTT CGACGACAGC TTATAAAAAC AAAGACTACG 
GGTAAAGCAA ATGAGGATTT GGGCAAGTAT GCGAAAGCTC TTGATGTCGC TATCATGCAA 
CTACATTCAA TGAAAATGAA TGAGATCAAT CGAATAGTAG ACGAACTTTG GAAACAAACC 
TACTGTGGAA CTGACATTGA TACCATTTTA ATTCGCTCTG ATAGTGAAGG TAAAGGGAAT 
AGGACTTACA ATTATAGAGT CTGTATGGTT AAAGGTGATG CCGAGCTAGA TATGCGAGGC 
CGCTGCAGTG CTGGTCAAAA GGTTTTAGCG TGTATTATTA TAAGGTTGGC TTTGGCAGAA 
TGTCTAGGTG TAAACTGTGG AATTTTGGCT CTGGATGAGC CAACTACAAA TCTCGATGAA 
GAGAATATAT GTAGTTTGGC TAAGAATTTA TCTCGGATTG TCGAATTTAG AAGGAAACAA 
GCAAACTTCC AACTCATTGT TATTACGCAT GATGAACAAT TTATCCGGTT AGTTAATTCA 
GATGCTTATT GTAGCTATTA TTATAGGGTG AAACGGGACA CGAATCAGAA GAGCATGATT 
GTTAAAGAAC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713090.2
CDS	168..4025
Translation	MSCIDRMSIMGIRSFDNRSRESIQFFSPLTLIVGQNGSGKTTIIECLKYATTGILPPNTKGGAFIHDPKICGEKEVLAQVKLAFRNTNQVKMICTRSLQLSVKKTTRQQKTLDGQLLILKDNERTTISNRCAELDSQVPLSLGVSKALLDYVIFCHQEESFWPLSEPANLKKRFDEIFESLRYAKALDQIKGLKRDQETQVKVDQATLTHYRSDKERAEKIELRVHESLKRISCIRSKVEELDQEITETARLQDELFKSTEEYEQQMITIRHLESQSDIINTTINDLKSQMTITDESSEDLEKLHSNFAEKVKEEQELYKSLEKKRSDLESLLKSRRELLEKLTGDLGKIQGEIESLEKLKVKKSTMINEIVHRYNINEINEEGIMTEVSKYASLVNKNYEISSGKLKERQVAVRARIEGIKAHEMFLNNRVSEINSSLEKQLTTQKELRSRFEILFPVKLQREDFTKDVEKSDLWIKSLRQEYESKNLLELLDKHQTALSSVENRLDEISEIVDSYHKYSGVRTKLQVFEENKTNKSAILANQLMTLKSSFSEVMSYELKDDDNYNEELDKLVEDVRKKLQEKEEAESSLRSVRERLEIRISLSVQSINDLTENKKIKTKTLKSYSGTFASMISEIKALESEIEENRKTLHSLQFGSTFYEKAIEICVDQHACQLCQRSLDKEEEKLFVEHCHSMIDVIPSKSAEVYSHLETLTKTFKNLSEAKPIFDEIELLDKRLSETKTELSDLQGDLQGLDIRKDEIQSELDTLYLRRANLEKLQLLVKDISNLEEEIRTIDRETEVLRIELPSSIAHHNLDEIYAEREKLLEKRGYLRKQIERTKLEETSFKKKIDDAVLANNEQKLKLTKLNFQVNELEQLEKDINKSSEDCDLQKKKLLEVSSKQGSQAPFLNELESEYEKLEADIQEMAQKSRTEILEANEYLHQLNEWNSELRIDVSTKFKCIKEKKSNIGEEVRIIASKIESTDDNLRKLQERLADLRTRERNASDNLRLRALMRQLEEAVTQKNYLLSQQSHDDRESFRERMQILKSKYGALNAERAGLLGECKQLENSITKDKEELNMEFKDADERFRRQLIKTKTTGKANEDLGKYAKALDVAIMQLHSMKMNEINRIVDELWKQTYCGTDIDTILIRSDSEGKGNRTYNYRVCMVKGDAELDMRGRCSAGQKVLACIIIRLALAECLGVNCGILALDEPTTNLDEENICSLAKNLSRIVEFRRKQANFQLIVITHDEQFIRLVNSDAYCSYYYRVKRDTNQKSMIVKEPL

Target ORF information:

RefSeq Version	XM_001713038.3
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- DNA repair protein Rad50 (rad50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001713038.3

ORF Insert Sequence:

ATGTCGTGCA TTGACAGAAT GTCCATCATG GGCATACGGT CGTTTGACAA TCGTTCACGA 
GAAAGCATTC AATTTTTCTC CCCATTAACG CTAATAGTAG GACAAAATGG GTCTGGAAAG 
ACTACAATAA TTGAATGCTT AAAGTACGCA ACAACAGGAA TACTCCCCCC AAATACAAAA 
GGTGGAGCCT TCATTCATGA TCCAAAAATA TGCGGTGAAA AAGAGGTCCT TGCTCAGGTC 
AAATTGGCGT TTCGAAATAC GAACCAAGTT AAGATGATCT GTACCAGATC ATTACAATTA 
TCTGTTAAAA AAACTACTCG ACAACAGAAA ACCTTGGACG GACAATTGCT AATATTGAAA 
GACAATGAAA GAACTACAAT TAGCAATCGT TGTGCCGAAT TAGATTCTCA AGTACCGCTC 
AGCTTGGGTG TGAGTAAAGC TTTATTAGAC TATGTCATTT TTTGTCATCA GGAAGAGAGC 
TTCTGGCCAT TAAGTGAGCC TGCTAATTTA AAAAAAAGGT TTGATGAGAT ATTTGAATCA 
TTGCGATATG CAAAGGCTCT GGATCAAATA AAGGGTTTAA AGCGCGACCA GGAAACTCAA 
GTGAAGGTCG ATCAAGCGAC ATTGACACAT TACCGCTCAG ACAAAGAAAG AGCCGAGAAA 
ATTGAATTGA GGGTACATGA ATCTTTGAAG CGTATATCCT GTATCCGATC TAAAGTCGAA 
GAGCTTGACC AAGAGATAAC TGAAACGGCT AGGCTACAAG ATGAGCTTTT CAAATCCACT 
GAAGAATATG AACAACAAAT GATTACAATC AGACATTTGG AAAGTCAAAG TGACATTATT 
AATACTACTA TCAACGACCT TAAAAGTCAA ATGACTATAA CTGACGAAAG TTCTGAAGAT 
TTGGAGAAAC TTCATTCGAA TTTTGCTGAA AAGGTTAAAG AGGAACAGGA ACTTTACAAA 
TCTTTAGAGA AGAAGCGTTC TGATTTAGAA AGTCTCCTCA AATCAAGAAG GGAACTCCTT 
GAAAAACTCA CTGGAGATTT GGGTAAAATC CAAGGAGAGA TAGAAAGCTT GGAGAAATTG 
AAGGTAAAAA AATCGACTAT GATTAACGAA ATTGTTCATC GCTACAATAT TAATGAAATA 
AATGAAGAAG GGATCATGAC AGAGGTGTCT AAATACGCTA GCCTTGTAAA TAAAAATTAT 
GAGATTTCCA GTGGTAAACT AAAAGAGCGA CAGGTGGCCG TGAGAGCACG CATCGAAGGA 
ATTAAAGCTC ACGAGATGTT CCTTAATAAT CGTGTTTCGG AAATCAACTC TTCACTTGAA 
AAACAACTCA CAACTCAAAA AGAATTGCGC TCACGCTTTG AAATCCTTTT TCCTGTAAAG 
TTACAGCGAG AAGATTTTAC TAAAGATGTT GAAAAATCGG ATTTGTGGAT TAAATCATTG 
CGTCAAGAGT ATGAAAGTAA AAATTTGCTG GAGTTACTTG ACAAACATCA AACAGCTCTG 
TCTTCAGTTG AAAACCGTTT AGATGAAATT TCAGAAATCG TCGATTCATA CCATAAATAC 
TCTGGAGTTC GTACCAAGTT ACAAGTTTTC GAAGAGAATA AAACCAATAA ATCTGCTATA 
CTGGCTAACC AACTGATGAC ATTGAAAAGT TCGTTTTCAG AAGTTATGTC TTACGAATTA 
AAGGATGATG ATAATTACAA TGAAGAATTG GATAAACTAG TCGAAGATGT ACGTAAAAAG 
TTACAAGAAA AAGAAGAGGC CGAATCATCA CTTCGGTCCG TCAGGGAAAG ACTTGAAATT 
AGAATTTCGT TATCTGTACA AAGTATTAAT GATTTAACCG AGAATAAGAA GATAAAAACT 
AAAACTTTGA AATCTTATAG TGGGACCTTC GCTTCTATGA TATCTGAAAT TAAAGCTTTG 
GAAAGTGAAA TAGAAGAAAA TAGGAAAACC CTTCACTCAT TGCAATTTGG ATCTACATTT 
TATGAGAAAG CTATAGAAAT ATGTGTTGAC CAACATGCTT GTCAACTATG TCAAAGAAGC 
CTCGATAAAG AAGAGGAAAA GCTCTTTGTG GAACACTGTC ACTCCATGAT TGACGTTATA 
CCATCCAAAT CTGCTGAAGT TTATAGTCAC CTTGAAACCC TTACTAAAAC TTTTAAAAAT 
CTTTCTGAAG CTAAACCGAT TTTCGATGAG ATTGAACTGC TTGATAAAAG GCTTTCAGAG 
ACGAAAACTG AACTTTCAGA TTTGCAAGGT GATCTACAAG GTTTGGATAT ACGTAAGGAC 
GAAATTCAAT CAGAATTGGA TACCTTGTAT TTAAGAAGAG CAAACTTAGA GAAATTACAA 
TTGTTGGTTA AAGACATTTC TAATTTAGAG GAAGAAATTC GTACAATTGA CAGAGAAACT 
GAAGTTTTAC GTATTGAACT TCCTTCTTCT ATTGCTCATC ATAATTTAGA CGAAATTTAT 
GCTGAGAGAG AAAAATTACT CGAGAAGAGG GGATACTTAA GGAAACAAAT AGAAAGAACT 
AAATTGGAGG AAACTTCTTT TAAAAAGAAA ATAGACGATG CTGTCTTAGC CAATAACGAG 
CAGAAACTAA AACTTACCAA GTTGAATTTT CAGGTCAATG AATTGGAACA ACTCGAAAAA 
GATATTAACA AATCCTCCGA AGACTGTGAC CTTCAAAAGA AAAAGCTTTT GGAAGTTAGC 
TCTAAACAAG GTTCTCAGGC ACCCTTTTTA AACGAATTAG AGTCAGAGTA TGAAAAATTA 
GAAGCAGATA TTCAAGAAAT GGCTCAAAAA AGTCGGACAG AAATCCTTGA AGCTAATGAA 
TACTTGCATC AACTTAATGA ATGGAACTCT GAATTGAGGA TTGACGTTTC CACAAAGTTT 
AAATGCATAA AAGAGAAGAA AAGTAATATT GGAGAAGAAG TTAGGATTAT TGCTTCTAAA 
ATTGAAAGTA CTGATGATAA TTTAAGAAAG TTACAGGAGC GCTTAGCGGA TTTGCGCACG 
AGGGAAAGGA ACGCATCTGA TAATCTACGC CTTCGCGCTT TAATGAGACA ATTAGAAGAA 
GCCGTGACGC AAAAGAATTA TTTGTTATCC CAGCAATCAC ATGATGATCG GGAATCATTT 
CGCGAACGAA TGCAAATTTT AAAGTCAAAA TACGGGGCAC TCAACGCTGA GCGCGCAGGC 
TTATTAGGAG AATGTAAGCA ATTGGAGAAC TCCATTACTA AAGACAAGGA AGAACTTAAT 
ATGGAGTTTA AAGATGCGGA CGAAAGATTT CGACGACAGC TTATAAAAAC AAAGACTACG 
GGTAAAGCAA ATGAGGATTT GGGCAAGTAT GCGAAAGCTC TTGATGTCGC TATCATGCAA 
CTACATTCAA TGAAAATGAA TGAGATCAAT CGAATAGTAG ACGAACTTTG GAAACAAACC 
TACTGTGGAA CTGACATTGA TACCATTTTA ATTCGCTCTG ATAGTGAAGG TAAAGGGAAT 
AGGACTTACA ATTATAGAGT CTGTATGGTT AAAGGTGATG CCGAGCTAGA TATGCGAGGC 
CGCTGCAGTG CTGGTCAAAA GGTTTTAGCG TGTATTATTA TAAGGTTGGC TTTGGCAGAA 
TGTCTAGGTG TAAACTGTGG AATTTTGGCT CTGGATGAGC CAACTACAAA TCTCGATGAA 
GAGAATATAT GTAGTTTGGC TAAGAATTTA TCTCGGATTG TCGAATTTAG AAGGAAACAA 
GCAAACTTCC AACTCATTGT TATTACGCAT GATGAACAAT TTATCCGGTT AGTTAATTCA 
GATGCTTATT GTAGCTATTA TTATAGGGTG AAACGGGACA CGAATCAGAA GAGCATGATT 
GTTAAAGAAC CTCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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rad50 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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