ALR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ALR1
Entrez Gene ID	853990
Full Name	Mg(2+) transporter ALR1
Synonyms	SWC3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183384.1	NP_014511.1	Mg(2+) transporter ALR1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E07261g	XP_454276.1
Saccharomyces cerevisiae (baker's yeast)	ALR1	NP_014511.1
Saccharomyces cerevisiae (baker's yeast)	ALR2	NP_116604.1
Eremothecium gossypii	AGOS_ADL171W	NP_983925.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Superoxide is released at the level of Erv/ALR monomers and does not require the obligatory involvement of dimeric forms of these proteins as they reduce the superoxide ion and release up to ~30% of the oxygen.
Title: Flavin-linked Erv-family sulfhydryl oxidases release superoxide anion during catalytic turnover.

Data conclude that variation in Alr1 protein accumulation or location does not make a substantial contribution to its regulation by Mg supply, suggesting Alr1 activity is directly regulated via as yet unknown mechanisms.
Title: Regulation of Alr1 Mg transporter activity by intracellular magnesium.

Alr1p and Alr2p are shown here to be present in the plasma membrane and shown to form homo-oligomers as well as hetero-oligomers; both the N-terminus and C-terminus of Alr1p and Alr2p are exposed to the inner side of the plasma membrane
Title: Oligomerization of the Mg2+-transport proteins Alr1p and Alr2p in yeast plasma membrane.

High expression of inactive mutants inhibited the capability of wild-type Alr1 to transport magnesium, consistent with Alr1 forming homo-oligomers. The results confirm the classification of ALR1 as a member of the CorA family of magnesium transport genes.
Title: Residues of the yeast ALR1 protein that are critical for magnesium uptake.

The following ALR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03166	NM_001183384.1 Latest version!	Saccharomyces cerevisiae S288C Mg(2+) transporter ALR1 (ALR1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALR1 ( NM_001183384.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014511.1 Saccharomyces cerevisiae S288C Mg(2+) transporter ALR1 (ALR1), partial mRNA.

Price & Availability↑

CloneID	OSi03166
Clone ID Related Accession (Same CDS sequence)	NM_001183384.1
Accession Version	NM_001183384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Mg(2+) transporter ALR1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCATCAT CCTCAAGTTC ATCAGAGTCA TCGCCTAATT TATCGCGGTC AAACTCACTG 
GCTAACACGA TGGTTTCCAT GAAAACAGAA GACCATACGG GATTGTACGA CCATAGGCAG 
CACCCTGACT CACTGCCGGT CCGTCACCAA CCTCCAACAT TGAAGAACAA GGAAATTGCT 
AAGTCCACTA AACCTTCTAT TCCTAAGGAG CAGAAGAGTG CAACGAGATA CAACAGCCAT 
GTGGATGTGG GAAGCGTACC CAGTAGGGGC CGCATGGATT TTGAAGATGA AGGGCAGGGT 
ATGGATGAAA CGGTTGCTCA TCATCAATTG AGGGCATCTG CCATTTTAAC TTCTAACGCG 
AGGCCTTCCA GACTAGCCCA CTCCATGCCA CATCAAAGGC AGCTTTATGT GGAAAGTAAT 
ATACACACAC CACCAAAGGA TGTAGGTGTA AAGAGAGACT ATACTATGTC ATCTTCTACT 
GCATCATCAG GTAACAAATC CAAGCTGAGC GCATCTTCCT CGGCAAGTCC TATTACGAAG 
GTTAGAAAAT CTTCGCTGGT CAGTCCTGTT CTTGAGATCC CACACGAATC GAAATCTGAT 
ACACACTCCA AGCTGGCTAA ACCCAAGAAG AGGACATATT CTACCACATC TGCGCATTCA 
TCTATCAATC CCGCAGTTTT ACTGACGAAA AGCACATCCC AAAAATCGGA TGCAGATGAT 
GATACATTGG AAAGAAAACC AGTACGTATG AATACTAGAG CTTCTTTTGA TAGTGACGTA 
TCACAGGCGT CGAGAGATTC TCAAGAGACG GAAGAAGATG TTTGTTTCCC TATGCCACCA 
CAATTGCATA CCAGAGTTAA CGGTATAGAT TTCGATGAAC TGGAAGAGTA CGCTCAGTTT 
GCAAACGCGG AGAAGAGTCA ATTTTTGGCA AGTCTGCAAG TGCCCAACGA ACAGAAGTAC 
AGTAACGTTT CTCAGGATAT CGGGTTTACC AGCTCTACTT CCACTTCGGG ATCATCAGCT 
GCCCTAAAAT ATACACCAAG AGTTTCGCAG ACTGGTGAAA AGAGCGAGAG TACCAATGAA 
ACAGAAATCC ATGAAAAGAA GGAAGACGAG CATGAGAAAA TCAAGCCTAG TTTACACCCT 
GGTATATCTT TCGGTAAAAA TAAAGTTGAA GGGGAAGAGA ATGAAAACAT TCCATCCAAT 
GACCCGGCGT ATTGCTCTTA CCAGGGTACA GACTTTCAAA TTCCTAATAG ATTTTCTTTT 
TTCTGTTCCG AATCTGATGA AACCGTTCAT GCTAGTGATA TCCCTTCCTT AGTGTCTGAG 
GGACAAACAT TCTATGAATT ATTCAGGGGA GGTGAGCCAA CATGGTGGTT AGATTGTAGT 
TGTCCAACTG ATGATGAAAT GCGTTGCATA GCAAAGGCGT TTGGTATCCA TCCATTGACT 
GCAGAAGATA TCAGAATGCA AGAAACACGT GAAAAGGTGG AACTTTTCAA ATCGTATTAC 
TTCGTTTGTT TCCATACCTT TGAAAATGAT AAAGAATCAG AGGATTTCCT GGAACCTATT 
AACGTTTACA TTGTTGTCTG TAGGTCAGGT GTTTTAACAT TCCATTTTGG CCCAATATCT 
CATTGTGCTA ATGTCAGAAG GCGTGTAAGA CAGTTACGTG ACTATGTCAA TGTCAATTCA 
GATTGGTTAT GTTATGCTTT AATCGATGAC ATTACTGATA GTTTCGCTCC TGTAATTCAA 
TCTATTGAAT ATGAAGCAGA TGCAATCGAA GATTCTGTGT TCATGGCTCG TGACATGGAT 
TTTGCAGCAA TGTTACAAAG AATTGGTGAA AGTAGGCGTA AGACAATGAC GTTGATGAGA 
CTTCTTAGTG GTAAGGCTGA TGTTATCAAA ATGTTTGCCA AAAGATGCCA AGATGAAGCT 
AATGGTATTG GACCGGCACT AACATCTCAA ATAAATATTG CTAACTTACA GGCAAGGCAG 
GATAACGCCA GTCATATCAA AAATAATAGT TCGACAACTG TACCAAATAA TTATGCGCCC 
ACCACATCAC AACCGAGGGG GGATATCGCC CTATACTTGG GTGATATTCA AGATCATTTG 
TTGACAATGT TTCAAAATTT GCTGGCGTAC GAAAAAATTT TTTCTAGATC CCATACAAAC 
TACTTAGCTC AACTGCAAGT GGAATCATTT AATTCTAATA ATAAAGTGAC AGAAATGTTG 
GGTAAAGTCA CCATGATAGG TACAATGCTA GTTCCATTAA ATGTTATCAC TGGTCTTTTC 
GGTATGAATG TTAAAGTTCC GGGGGAGAAT TCCAGTATCG CGTGGTGGTT TGGGATATTG 
GGTGTGCTGC TTTTGCTGGC CGTCCTTGGT TGGTTCTTGG CCAGCTATTG GATTAAAAGA 
ATAGACCCTC CTGCAACACT TAACGAGGCC GCTGAAAGTG GAGCCAAATC GGTTATTTCG 
AGCTTCTTGC CAAAAAGAAA CAAGAGATTC AATGATCGTT CCAAAAATAT AAATGTAAGA 
GCCGGTCCTT CAAACAAATC AGTTGCAAGT CTTCCTAGTA GATATAGCCG CTACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014511.1
CDS	1..2580
Translation	MSSSSSSSESSPNLSRSNSLANTMVSMKTEDHTGLYDHRQHPDSLPVRHQPPTLKNKEIAKSTKPSIPKEQKSATRYNSHVDVGSVPSRGRMDFEDEGQGMDETVAHHQLRASAILTSNARPSRLAHSMPHQRQLYVESNIHTPPKDVGVKRDYTMSSSTASSGNKSKLSASSSASPITKVRKSSLVSPVLEIPHESKSDTHSKLAKPKKRTYSTTSAHSSINPAVLLTKSTSQKSDADDDTLERKPVRMNTRASFDSDVSQASRDSQETEEDVCFPMPPQLHTRVNGIDFDELEEYAQFANAEKSQFLASLQVPNEQKYSNVSQDIGFTSSTSTSGSSAALKYTPRVSQTGEKSESTNETEIHEKKEDEHEKIKPSLHPGISFGKNKVEGEENENIPSNDPAYCSYQGTDFQIPNRFSFFCSESDETVHASDIPSLVSEGQTFYELFRGGEPTWWLDCSCPTDDEMRCIAKAFGIHPLTAEDIRMQETREKVELFKSYYFVCFHTFENDKESEDFLEPINVYIVVCRSGVLTFHFGPISHCANVRRRVRQLRDYVNVNSDWLCYALIDDITDSFAPVIQSIEYEADAIEDSVFMARDMDFAAMLQRIGESRRKTMTLMRLLSGKADVIKMFAKRCQDEANGIGPALTSQINIANLQARQDNASHIKNNSSTTVPNNYAPTTSQPRGDIALYLGDIQDHLLTMFQNLLAYEKIFSRSHTNYLAQLQVESFNSNNKVTEMLGKVTMIGTMLVPLNVITGLFGMNVKVPGENSSIAWWFGILGVLLLLAVLGWFLASYWIKRIDPPATLNEAAESGAKSVISSFLPKRNKRFNDRSKNINVRAGPSNKSVASLPSRYSRYD

Target ORF information:

RefSeq Version	NM_001183384.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Mg(2+) transporter ALR1 (ALR1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183384.1

ORF Insert Sequence:

ATGTCATCAT CCTCAAGTTC ATCAGAGTCA TCGCCTAATT TATCGCGGTC AAACTCACTG 
GCTAACACGA TGGTTTCCAT GAAAACAGAA GACCATACGG GATTGTACGA CCATAGGCAG 
CACCCTGACT CACTGCCGGT CCGTCACCAA CCTCCAACAT TGAAGAACAA GGAAATTGCT 
AAGTCCACTA AACCTTCTAT TCCTAAGGAG CAGAAGAGTG CAACGAGATA CAACAGCCAT 
GTGGATGTGG GAAGCGTACC CAGTAGGGGC CGCATGGATT TTGAAGATGA AGGGCAGGGT 
ATGGATGAAA CGGTTGCTCA TCATCAATTG AGGGCATCTG CCATTTTAAC TTCTAACGCG 
AGGCCTTCCA GACTAGCCCA CTCCATGCCA CATCAAAGGC AGCTTTATGT GGAAAGTAAT 
ATACACACAC CACCAAAGGA TGTAGGTGTA AAGAGAGACT ATACTATGTC ATCTTCTACT 
GCATCATCAG GTAACAAATC CAAGCTGAGC GCATCTTCCT CGGCAAGTCC TATTACGAAG 
GTTAGAAAAT CTTCGCTGGT CAGTCCTGTT CTTGAGATCC CACACGAATC GAAATCTGAT 
ACACACTCCA AGCTGGCTAA ACCCAAGAAG AGGACATATT CTACCACATC TGCGCATTCA 
TCTATCAATC CCGCAGTTTT ACTGACGAAA AGCACATCCC AAAAATCGGA TGCAGATGAT 
GATACATTGG AAAGAAAACC AGTACGTATG AATACTAGAG CTTCTTTTGA TAGTGACGTA 
TCACAGGCGT CGAGAGATTC TCAAGAGACG GAAGAAGATG TTTGTTTCCC TATGCCACCA 
CAATTGCATA CCAGAGTTAA CGGTATAGAT TTCGATGAAC TGGAAGAGTA CGCTCAGTTT 
GCAAACGCGG AGAAGAGTCA ATTTTTGGCA AGTCTGCAAG TGCCCAACGA ACAGAAGTAC 
AGTAACGTTT CTCAGGATAT CGGGTTTACC AGCTCTACTT CCACTTCGGG ATCATCAGCT 
GCCCTAAAAT ATACACCAAG AGTTTCGCAG ACTGGTGAAA AGAGCGAGAG TACCAATGAA 
ACAGAAATCC ATGAAAAGAA GGAAGACGAG CATGAGAAAA TCAAGCCTAG TTTACACCCT 
GGTATATCTT TCGGTAAAAA TAAAGTTGAA GGGGAAGAGA ATGAAAACAT TCCATCCAAT 
GACCCGGCGT ATTGCTCTTA CCAGGGTACA GACTTTCAAA TTCCTAATAG ATTTTCTTTT 
TTCTGTTCCG AATCTGATGA AACCGTTCAT GCTAGTGATA TCCCTTCCTT AGTGTCTGAG 
GGACAAACAT TCTATGAATT ATTCAGGGGA GGTGAGCCAA CATGGTGGTT AGATTGTAGT 
TGTCCAACTG ATGATGAAAT GCGTTGCATA GCAAAGGCGT TTGGTATCCA TCCATTGACT 
GCAGAAGATA TCAGAATGCA AGAAACACGT GAAAAGGTGG AACTTTTCAA ATCGTATTAC 
TTCGTTTGTT TCCATACCTT TGAAAATGAT AAAGAATCAG AGGATTTCCT GGAACCTATT 
AACGTTTACA TTGTTGTCTG TAGGTCAGGT GTTTTAACAT TCCATTTTGG CCCAATATCT 
CATTGTGCTA ATGTCAGAAG GCGTGTAAGA CAGTTACGTG ACTATGTCAA TGTCAATTCA 
GATTGGTTAT GTTATGCTTT AATCGATGAC ATTACTGATA GTTTCGCTCC TGTAATTCAA 
TCTATTGAAT ATGAAGCAGA TGCAATCGAA GATTCTGTGT TCATGGCTCG TGACATGGAT 
TTTGCAGCAA TGTTACAAAG AATTGGTGAA AGTAGGCGTA AGACAATGAC GTTGATGAGA 
CTTCTTAGTG GTAAGGCTGA TGTTATCAAA ATGTTTGCCA AAAGATGCCA AGATGAAGCT 
AATGGTATTG GACCGGCACT AACATCTCAA ATAAATATTG CTAACTTACA GGCAAGGCAG 
GATAACGCCA GTCATATCAA AAATAATAGT TCGACAACTG TACCAAATAA TTATGCGCCC 
ACCACATCAC AACCGAGGGG GGATATCGCC CTATACTTGG GTGATATTCA AGATCATTTG 
TTGACAATGT TTCAAAATTT GCTGGCGTAC GAAAAAATTT TTTCTAGATC CCATACAAAC 
TACTTAGCTC AACTGCAAGT GGAATCATTT AATTCTAATA ATAAAGTGAC AGAAATGTTG 
GGTAAAGTCA CCATGATAGG TACAATGCTA GTTCCATTAA ATGTTATCAC TGGTCTTTTC 
GGTATGAATG TTAAAGTTCC GGGGGAGAAT TCCAGTATCG CGTGGTGGTT TGGGATATTG 
GGTGTGCTGC TTTTGCTGGC CGTCCTTGGT TGGTTCTTGG CCAGCTATTG GATTAAAAGA 
ATAGACCCTC CTGCAACACT TAACGAGGCC GCTGAAAGTG GAGCCAAATC GGTTATTTCG 
AGCTTCTTGC CAAAAAGAAA CAAGAGATTC AATGATCGTT CCAAAAATAT AAATGTAAGA 
GCCGGTCCTT CAAACAAATC AGTTGCAAGT CTTCCTAGTA GATATAGCCG CTACGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ALR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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