BFA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BFA1
Entrez Gene ID	853513
Full Name	Bfa1p
Synonyms	IBD1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181711.3	NP_012587.3	Bfa1p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	BFA1	NP_012587.3
Kluyveromyces lactis	KLLA0A08041g	XP_451358.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tem1 activity is tightly controlled in mitosis by Bub2 and Bfa1 and is also regulated by the spindle orientation checkpoint that monitors the correct alignment of the mitotic spindle with the mother-daughter axis. Here is described the purification of Tem1, Bfa1, and Bub2 and a detailed radioactive filter-binding assay to study the nucleotide binding properties of Tem1 and the role of its regulators Bfa1 and Bub2.
Title: In Vitro Analysis of Tem1 GTPase Activity and Regulation by the Bfa1/Bub2 GAP.

Bmh1 is a spindle position checkpoint (SPOC) component that breaks Bfa1 centrosome asymmetry upon SPOC activation.
Title: The 14-3-3 protein Bmh1 functions in the spindle position checkpoint by breaking Bfa1 asymmetry at yeast centrosomes.

Cdc5 induces the asymmetric distribution of Bfa1 to the bud-directed spindle pole body independently of Bfa1 GAP activity at anaphase.
Title: Cdc5-dependent asymmetric localization of bfa1 fine-tunes timely mitotic exit.

A link is confirmed between the subcellular localization pattern of Bfa1 at the spindle pole bodies with cell cycle progression.
Title: Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1.

there is a GAP-independent surveillance mechanism of Bfa1/Bub2, which, together with the GTP/GDP switch of Tem1, may be required for the genomic stability of cells with misaligned spindles
Title: Different levels of Bfa1/Bub2 GAP activity are required to prevent mitotic exit of budding yeast depending on the type of perturbations.

The following BFA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BFA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02749	NM_001181711.3 Latest version!	Saccharomyces cerevisiae S288C Bfa1p (BFA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BFA1 ( NM_001181711.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012587.3 Saccharomyces cerevisiae S288C Bfa1p (BFA1), partial mRNA.

Price & Availability↑

CloneID	OSi02749
Clone ID Related Accession (Same CDS sequence)	NM_001181711.3
Accession Version	NM_001181711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Bfa1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Jul 30, 2012 this sequence version replaced NM_001181711.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAATTA GGCCTCTCAC GTTAAACGGT TTAGATGAGC CAGAAACCTC TTTTGAAGAA 
CTGAATACAA CTCTACCTCG CTTTCAATCC CATGAAACAT TAACTTTGGA AGAAAACGTG 
CCACCATTGA GTACATCAAC TTATATACCG CCTCCATCCT CGGTAGGTAC TTCTGACACT 
GGCACGGTAT TTTCCAATAG TACCAGCGCG TTTTGGTCTA ATAAGCAAGC AGACGATGAT 
CAGGACATGG AGGTAGACCA GGATGATGAA TTTCTAAATG ACTTCCAGGA ATTCCAAAAT 
AAAAAGGATG ATTTTGACGA TGCTATTAAA ACAAATTTTC ATTTGAGAAA TGGATGTCGA 
ACTGGCCCTT TCAAAAACGA CATTTTCGCA GAAGAGTTTG ATAGAAAACT GAGTTTGGAA 
GATAAACCAA GGCTAAAGCA ACCTAGATCA ATGATGGAAT TGAAACCAAA AAGAAAACTA 
TCAAATAGCG TCACTTCAAG AAACTTAAGG TCTGGTAATT CTGTAAGATT TAAGAAATCT 
ATGCCTAACT TAGCTTTAGT TAATCCAGCT ATCAGAGAAG AAGAAGAGGA CGAAGAACGA 
GAAAGAGAAG ATCAACGAGA GTTTAATTAC AAGATTGATA ACGATACGCA GGACACAATT 
TTGGCGAAAT TCAGTTCAGA TGATGAAGGT GACTTTCTGA CAGGATTTGA AGAATTAGAG 
GGTGAAGCAA TTGATGAAAC GATTTCTTCG AACGATAAAG AAAGTGCCGA CCATCCGCGT 
TTCTTAAAGA AGTCATCCTC ATCTTTGCCA TTAAAAATAT CACCTGCACA ATATGATATA 
GTAAAGCATG ACGAATTATT AACTCCAGGT CTTCATAGGC GACAACGAGA TTGGAATACC 
CAGCAGGAAC TGGACTCTTT TAAAGAAAAA CGATCAGTAA GACACTGTTC TAATCAAAAT 
GTACAACTCA ATGGTCCAGC TAAAATCAAA ACAATAAAAC AGCAAATTGA TCATAATACT 
CCAATGAAGA AAGGTTCTAT GATATATAAC CCGAAAACTA TGAAGTGGGA AGGAAATGAA 
AACGTCTTAA GCAAATTTAG TGATGTAGAC ACGGCAAACA GGAAAGCTTT ATTGATAAAA 
AACAAGCTAC AACGAGATGC AGATTCTAAG AAGCAAAAAT ATTCTGATCT ACAGCATGCA 
AGAGCCACTT CGAGAAATCA AAAGGTCATC GGGAACATGA TTTTAGATGA ACAAAATTTA 
AGATGGGTTA GTGTTTCAGA GGAAGAAGCA GACCCTTTTG CAGGCATTCC TGAGATAAAC 
TTACCACCAG TTGGCAAAAG TATGAAAAAA CGTAGCTCCT CACCATTTTT GCGTTCCAAA 
AGCCAAGTTA ATACGCCATT TGTTTCAAAT GACAATGATG GCGTTTATCA AAGTACGGCT 
GCTCAAGCAA GACTCCGTAA ATACCATTCA ATGAGGACAC TTAACGGTAC AACAGAAACT 
CCAGAAATAA GCTCTACTTT CCATTTGAGT TCAAGAGCAT TGGAAAAATT TTACCACGAG 
GAGAACAGGT GGTGTAAAAA GTTAGCCTCA TGGTTTATAC CCCGAGATGA GACCATTATC 
AGTGTTGATG AGGAAACAAT CATGGATGAA AGTACGGTCA ATAGCAAGAG AAAATCCTAT 
ATGTATGAAA TCAGGAACAT GGTAATCAAT TCGACAAAAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012587.3
CDS	1..1725
Translation	MSIRPLTLNGLDEPETSFEELNTTLPRFQSHETLTLEENVPPLSTSTYIPPPSSVGTSDTGTVFSNSTSAFWSNKQADDDQDMEVDQDDEFLNDFQEFQNKKDDFDDAIKTNFHLRNGCRTGPFKNDIFAEEFDRKLSLEDKPRLKQPRSMMELKPKRKLSNSVTSRNLRSGNSVRFKKSMPNLALVNPAIREEEEDEEREREDQREFNYKIDNDTQDTILAKFSSDDEGDFLTGFEELEGEAIDETISSNDKESADHPRFLKKSSSSLPLKISPAQYDIVKHDELLTPGLHRRQRDWNTQQELDSFKEKRSVRHCSNQNVQLNGPAKIKTIKQQIDHNTPMKKGSMIYNPKTMKWEGNENVLSKFSDVDTANRKALLIKNKLQRDADSKKQKYSDLQHARATSRNQKVIGNMILDEQNLRWVSVSEEEADPFAGIPEINLPPVGKSMKKRSSSPFLRSKSQVNTPFVSNDNDGVYQSTAAQARLRKYHSMRTLNGTTETPEISSTFHLSSRALEKFYHEENRWCKKLASWFIPRDETIISVDEETIMDESTVNSKRKSYMYEIRNMVINSTKD

Target ORF information:

RefSeq Version	NM_001181711.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Bfa1p (BFA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181711.3

ORF Insert Sequence:

ATGTCAATTA GGCCTCTCAC GTTAAACGGT TTAGATGAGC CAGAAACCTC TTTTGAAGAA 
CTGAATACAA CTCTACCTCG CTTTCAATCC CATGAAACAT TAACTTTGGA AGAAAACGTG 
CCACCATTGA GTACATCAAC TTATATACCG CCTCCATCCT CGGTAGGTAC TTCTGACACT 
GGCACGGTAT TTTCCAATAG TACCAGCGCG TTTTGGTCTA ATAAGCAAGC AGACGATGAT 
CAGGACATGG AGGTAGACCA GGATGATGAA TTTCTAAATG ACTTCCAGGA ATTCCAAAAT 
AAAAAGGATG ATTTTGACGA TGCTATTAAA ACAAATTTTC ATTTGAGAAA TGGATGTCGA 
ACTGGCCCTT TCAAAAACGA CATTTTCGCA GAAGAGTTTG ATAGAAAACT GAGTTTGGAA 
GATAAACCAA GGCTAAAGCA ACCTAGATCA ATGATGGAAT TGAAACCAAA AAGAAAACTA 
TCAAATAGCG TCACTTCAAG AAACTTAAGG TCTGGTAATT CTGTAAGATT TAAGAAATCT 
ATGCCTAACT TAGCTTTAGT TAATCCAGCT ATCAGAGAAG AAGAAGAGGA CGAAGAACGA 
GAAAGAGAAG ATCAACGAGA GTTTAATTAC AAGATTGATA ACGATACGCA GGACACAATT 
TTGGCGAAAT TCAGTTCAGA TGATGAAGGT GACTTTCTGA CAGGATTTGA AGAATTAGAG 
GGTGAAGCAA TTGATGAAAC GATTTCTTCG AACGATAAAG AAAGTGCCGA CCATCCGCGT 
TTCTTAAAGA AGTCATCCTC ATCTTTGCCA TTAAAAATAT CACCTGCACA ATATGATATA 
GTAAAGCATG ACGAATTATT AACTCCAGGT CTTCATAGGC GACAACGAGA TTGGAATACC 
CAGCAGGAAC TGGACTCTTT TAAAGAAAAA CGATCAGTAA GACACTGTTC TAATCAAAAT 
GTACAACTCA ATGGTCCAGC TAAAATCAAA ACAATAAAAC AGCAAATTGA TCATAATACT 
CCAATGAAGA AAGGTTCTAT GATATATAAC CCGAAAACTA TGAAGTGGGA AGGAAATGAA 
AACGTCTTAA GCAAATTTAG TGATGTAGAC ACGGCAAACA GGAAAGCTTT ATTGATAAAA 
AACAAGCTAC AACGAGATGC AGATTCTAAG AAGCAAAAAT ATTCTGATCT ACAGCATGCA 
AGAGCCACTT CGAGAAATCA AAAGGTCATC GGGAACATGA TTTTAGATGA ACAAAATTTA 
AGATGGGTTA GTGTTTCAGA GGAAGAAGCA GACCCTTTTG CAGGCATTCC TGAGATAAAC 
TTACCACCAG TTGGCAAAAG TATGAAAAAA CGTAGCTCCT CACCATTTTT GCGTTCCAAA 
AGCCAAGTTA ATACGCCATT TGTTTCAAAT GACAATGATG GCGTTTATCA AAGTACGGCT 
GCTCAAGCAA GACTCCGTAA ATACCATTCA ATGAGGACAC TTAACGGTAC AACAGAAACT 
CCAGAAATAA GCTCTACTTT CCATTTGAGT TCAAGAGCAT TGGAAAAATT TTACCACGAG 
GAGAACAGGT GGTGTAAAAA GTTAGCCTCA TGGTTTATAC CCCGAGATGA GACCATTATC 
AGTGTTGATG AGGAAACAAT CATGGATGAA AGTACGGTCA ATAGCAAGAG AAAATCCTAT 
ATGTATGAAA TCAGGAACAT GGTAATCAAT TCGACAAAAG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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BFA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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