GSC2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GSC2
Entrez Gene ID	852920
Full Name	1,3-beta-glucan synthase GSC2
Synonyms	FKS2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181161.3	NP_011546.3	1,3-beta-glucan synthase GSC2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	GSC2	NP_011546.3
Saccharomyces cerevisiae (baker's yeast)	FKS1	NP_013446.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ste11 regulates the FKS2 gene through its substrates and through Mpk1.
Title: Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene.

The dimeric complex of Mpk1 binds to a Swi4 recognition site in the FKS2 promoter.[Mpk1, Swi4, FKS2]
Title: Transcriptional reporters for genes activated by cell wall stress through a non-catalytic mechanism involving Mpk1 and SBF.

negative regulation of GSC2 by SMK1 is important for spore wall deposition
Title: The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae.

The following GSC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GSC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02263	NM_001181161.3 Latest version!	Saccharomyces cerevisiae S288C 1,3-beta-glucan synthase GSC2 (GSC2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GSC2 ( NM_001181161.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011546.3 Saccharomyces cerevisiae S288C 1,3-beta-glucan synthase GSC2 (GSC2), partial mRNA.

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CloneID	OSi02263
Clone ID Related Accession (Same CDS sequence)	NM_001181161.3
Accession Version	NM_001181161.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	1,3-beta-glucan synthase GSC2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181161.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCTACA ACGATCCAAA CTTGAATGGA CAGTATTACA GTAACGGTGA TGGGACTGGT 
GACGGTAATT ACCCTACGTA CCAAGTGACA CAGGATCAAA GTGCGTACGA TGAGTACGGT 
CAGCCAATCT ATACACAAAA CCAACTGGAT GATGGTTATT ATGATCCAAA CGAACAATAC 
GTTGACGGTA CACAATTTCC TCAGGGACAA GATCCTTCAC AAGACCAAGG TCCTTATAAT 
AACGATGCTA GTTACTATAA CCAACCCCCC AATATGATGA ACCCGTCTTC TCAAGATGGA 
GAGAACTTCT CAGATTTTAG CAGCTATGGT CCCCCATCCG GCACTTATCC TAACGATCAA 
TATACTCCTT CTCAAATGAG TTATCCTGAT CAAGATGGTT CTTCAGGGGC CTCAACCCCC 
TATGGAAATG GTGTCGTTAA TGGTAATGGC CAGTACTACG ACCCTAATGC TATTGAAATG 
GCTTTACCAA ATGATCCATA TCCCGCATGG ACCGCAGATC CCCAGTCTCC CCTGCCCATC 
GAACAAATCG AAGATATCTT CATAGATTTA ACAAATAAAT TCGGTTTTCA GAGAGATTCC 
ATGAGAAATA TGTTTGACCA TTTTATGACC CTTTTGGACT CTAGATCTTC TAGGATGTCT 
CCAGAACAGG CCCTTTTATC ATTACATGCA GACTACATAG GTGGAGATAC GGCCAACTAC 
AAAAAATGGT ACTTTGCCGC TCAACTTGAT ATGGATGATG AAATTGGTTT CAGGAATATG 
AAGTTGGGTA AGCTATCAAG AAAGGCAAGA AAGGCTAAGA AGAAAAATAA AAAAGCCATG 
CAAGAGGCTA GTCCTGAAGA CACTGAGGAG ACTTTAAATC AAATTGAGGG TGATAACTCA 
TTAGAAGCTG CGGATTTTAG ATGGAAGTCA AAGATGAATC AACTTTCTCC ATTTGAAATG 
GTTCGTCAAA TTGCCTTGTT TTTATTATGT TGGGGCGAGG CAAATCAAGT CAGATTTACC 
CCGGAGTGTC TTTGTTTCAT TTATAAATGC GCCTCTGATT ACTTAGATTC TGCACAATGT 
CAACAACGTC CTGATCCCTT GCCTGAAGGT GATTTTTTGA ATAGAGTTAT TACTCCTCTT 
TATCGTTTTA TTAGGAGCCA GGTTTACGAA ATCGTGGATG GTCGATACGT GAAGAGTGAA 
AAAGATCATA ACAAAGTTAT TGGGTATGAT GATGTCAATC AATTATTCTG GTATCCAGAA 
GGTATAGCAA AAATTGTCAT GGAAGATGGA ACCAGGTTGA TTGATTTGCC AGCAGAGGAG 
CGTTATTTGA AATTGGGAGA AATTCCCTGG GATGATGTCT TCTTTAAAAC TTACAAAGAA 
ACACGTTCCT GGTTACATTT AGTTACCAAC TTCAATCGTA TTTGGATCAT GCACATCTCA 
GTATATTGGA TGTATTGTGC TTACAATGCT CCAACTTTTT ATACTCACAA CTATCAACAA 
TTGGTCGACA ATCAGCCTTT GGCAGCTTAT AAATGGGCCA CTGCAGCATT AGGTGGTACT 
GTGGCAAGTT TGATTCAAGT TGCCGCTACT TTGTGCGAGT GGTCATTCGT TCCTAGAAAA 
TGGGCGGGTG CTCAACATTT GTCCCGTAGA TTCTGGTTCT TGTGTGTCAT TATGGGTATT 
AATTTGGGGC CTGTGATATT TGTTTTCGCT TATGATAAGG ACACAGTATA TTCTACTGCC 
GCTCATGTCG TTGGAGCAGT TATGTTTTTT GTTGCTGTGG CAACACTTGT TTTCTTTTCC 
GTAATGCCAT TGGGTGGATT ATTTACATCG TATATGAAAA AGTCCACAAG AAGTTATGTT 
GCCTCACAGA CCTTCACCGC ATCTTTTGCT CCATTGCATG GTTTAGACAG GTGGATGTCT 
TATTTGGTTT GGGTAACCGT TTTTGCTGCT AAATATGCAG AGTCATATTT TTTTCTAATA 
CTGTCACTAA GAGATCCAAT TAGGATTTTA TCTACTACAT CAATGAGATG TACTGGTGAA 
TACTGGTGGG GTAATAAGAT TTGTAAGGTC CAGCCAAAGA TTGTTTTAGG TTTAATGATT 
GCGACTGACT TCATTTTGTT CTTTTTGGAT ACCTACTTGT GGTATATCGT TGTTAACACT 
GTTTTCTCGG TCGGAAAATC GTTCTATTTG GGTATTTCTA TCTTAACTCC ATGGAGAAAT 
ATTTTCACTA GATTGCCAAA AAGAATTTAT TCTAAGATCT TGGCTACTAC TGATATGGAA 
ATAAAATATA AACCGAAAGT ACTAATTTCT CAGATTTGGA ATGCTATCAT TATCTCCATG 
TACAGAGAAC ATTTATTAGC CATAGACCAT GTACAAAAAT TGTTATATCA TCAGGTTCCG 
TCCGAAATTG AAGGTAAGAG GACTTTGAGA GCACCAACTT TCTTTGTTTC CCAAGATGAC 
AATAATTTTG AGACTGAATT TTTCCCTAGA GATTCAGAAG CTGAGCGCCG TATTTCATTT 
TTTGCCCAAT CTCTATCCAC TCCAATTCCA GAACCACTAC CAGTTGACAA CATGCCAACA 
TTTACTGTAT TAACTCCCCA TTACGCCGAG AGGATTCTAT TATCATTGAG AGAAATTATT 
CGTGAAGATG ATCAATTTTC AAGAGTTACT CTTTTGGAAT ACCTGAAGCA ATTACACCCG 
GTAGAATGGG ACTGTTTTGT TAAGGATACG AAAATTCTTG CTGAAGAAAC GGCCGCATAT 
GAAAACAATG AAGATGAACC TGAAAAGGAA GACGCTCTGA AATCTCAAAT TGATGATTTA 
CCTTTCTATT GTATTGGTTT CAAATCTGCT GCACCAGAAT ACACCTTACG TACGAGAATC 
TGGGCCTCTT TAAGGTCGCA AACTTTGTAT CGCACAATCT CGGGGTTTAT GAATTATTCG 
AGGGCCATAA AATTACTTTA TCGTGTGGAA AATCCAGAAA TCGTTCAAAT GTTCGGTGGT 
AATGCTGATG GATTAGAAAG AGAACTGGAA AAAATGGCAA GGCGAAAATT CAAATTCTTG 
GTTTCGATGC AAAGATTGGC CAAGTTTAAA CCACATGAAC TAGAAAATGC TGAGTTCCTG 
TTGAGAGCTT ATCCGGACTT GCAAATTGCC TACCTGGATG AAGAACCTCC CTTAAACGAA 
GGCGAAGAGC CAAGAATTTA CTCGGCCTTA ATTGATGGTC ATTGTGAGAT TTTAGAGAAT 
GGTCGTAGAC GTCCCAAATT TAGAGTTCAA CTATCCGGTA ATCCAATTCT TGGTGATGGT 
AAATCAGATA ATCAAAATCA TGCTTTGATT TTTTACAGAG GTGAGTATAT TCAATTGATT 
GATGCTAATC AAGACAATTA CTTGGAAGAG TGTTTGAAAA TCAGGTCTGT CTTAGCAGAA 
TTTGAAGAAT TGGGAATTGA GCAAATTCAT CCTTATACTC CTGGTTTAAA ATATGAGGAC 
CAATCCACAA ATCATCCTGT TGCAATTGTC GGCGCTAGAG AATATATTTT CTCAGAAAAC 
TCTGGTGTTC TTGGTGATGT AGCGGCTGGT AAAGAACAAA CTTTTGGTAC ATTATTTGCC 
CGTACTTTGG CACAGATTGG TGGTAAATTG CATTATGGTC ATCCAGATTT TATTAATGCG 
ACATTCATGA CTACTAGGGG TGGTGTTTCC AAAGCACAAA AGGGTCTACA TTTAAATGAA 
GATATTTATG CCGGTATGAA TGCCGTACTT CGGGGTGGTC GTATCAAGCA TTGCGAATAT 
TATCAGTGTG GTAAAGGTAG AGATTTAGGT TTTGGTACAA TTTTGAATTT CACTACTAAG 
ATCGGTGCTG GTATGGGTGA ACAAATGTTA TCTCGTGAAT ACTATTATTT GGGTACGCAA 
TTACCTATTG ACCGTTTTTT AACATTTTAT TATGCGCATC CAGGGTTTCA CTTGAATAAC 
TTATTTATTC AATTGTCTCT GCAGATGTTC ATGTTAACTT TAGTGAACTT GCATGCTTTG 
GCTCATGAAT CCATTCTGTG TGTTTACGAT AGGGATAAGC CAATTACTGA TGTTTTGTAT 
CCAATTGGTT GTTACAACTT TCATCCTGCG ATTGATTGGG TGAGACGTTA TACACTCTCT 
ATTTTCATCG TCTTTTGGAT TGCTTTTGTC CCTATTGTCG TTCAGGAATT AATCGAGCGT 
GGTCTGTGGA AGGCGACACA AAGATTTTTC CGTCACATTT TATCTCTATC TCCAATGTTT 
GAAGTCTTTG CTGGCCAAAT CTATTCTTCA GCACTGTTAA GTGATATCGC TGTGGGTGGT 
GCTCGTTATA TTTCAACAGG TCGTGGCTTT GCTACATCTC GTATACCTTT CTCTATTCTT 
TATTCAAGAT TTGCGGGTTC AGCCATTTAT ATGGGATCAA GATCAATGTT GATGTTATTA 
TTTGGTACCG TGGCACATTG GCAAGCTCCA CTATTATGGT TTTGGGCATC ATTATCAGCC 
TTAATCTTTG CACCATTCAT TTTCAATCCA CATCAATTTG CTTGGGAAGA TTTTTTCCTA 
GACTACAGAG ATTATATCAG ATGGCTGTCA AGAGGTAATA ATAAGTACCA CAGGAACTCA 
TGGATTGGTT ATGTAAGAAT GTCGAGGTCT CGTGTTACTG GTTTCAAGCG CAAACTGGTG 
GGTGATGAGT CTGAAAAATC TGCAGGCGAT GCAAGCAGGG CTCATAGAAC CAATTTAATT 
ATGGCTGAAA TTATACCGTG TGCGATTTAC GCAGCAGGTT GTTTTATTGC CTTCACGTTT 
ATTAATGCAC AAACTGGTGT CAAGACTACT GATGAAGATA GAGTAAATTC CACCTTACGT 
ATCATCATTT GCACCTTGGC GCCTATTGTT ATCGATATTG GTGTTTTATT CTTCTGTATG 
GGTTTGTCAT GCTGCTCTGG CCCTTTGTTG GGCATGTGCT GCAAGAAAAC TGGTTCTGTT 
ATGGCAGGGA TCGCTCACGG TATCGCTGTT GTTGTCCATA TTGTCTTTTT CATTGTCATG 
TGGGTTTTAG AGGGTTTTAG TTTTGTTAGG ATGTTGATTG GCGTTGTTAC ATGTATACAA 
TGTCAAAGGT TGATTTTTCA CTGTATGACT GTACTGTTGC TGACCCGTGA GTTCAAGAAT 
GATCACGCTA ATACTGCCTT TTGGACAGGC AAATGGTACA GCACCGGTTT AGGATATATG 
GCATGGACTC AACCGACAAG GGAATTGACT GCAAAAGTCA TTGAGCTTTC CGAGTTTGCA 
GCGGATTTTG TTTTGGGGCA TGTAATTTTG ATCTTCCAAC TACCAGTCAT TTGTATTCCA 
AAGATAGATA AGTTTCACTC CATCATGTTA TTTTGGTTAA AACCATCCCG TCAAATCCGT 
CCTCCTATTT ACTCTTTGAA ACAAGCACGC CTACGTAAAC GTATGGTTAG GAGGTATTGC 
AGCTTGTACT TTTTGGTACT GATCATATTC GCGGGATGCA TCGTTGGCCC TGCCGTTGCT 
TCAGCACATG TTCCAAAAGA CCTTGGATCT GGGTTGACGG GTACTTTCCA TAACTTGGTT 
CAACCAAGGA ACGTATCTAA CAATGATACA GGGTCCCAGA TGTCTACTTA TAAGAGTCAT 
TATTACACTC ATACGCCATC CTTAAAGACC TGGTCTACGA TCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011546.3
CDS	1..5688
Translation	MSYNDPNLNGQYYSNGDGTGDGNYPTYQVTQDQSAYDEYGQPIYTQNQLDDGYYDPNEQYVDGTQFPQGQDPSQDQGPYNNDASYYNQPPNMMNPSSQDGENFSDFSSYGPPSGTYPNDQYTPSQMSYPDQDGSSGASTPYGNGVVNGNGQYYDPNAIEMALPNDPYPAWTADPQSPLPIEQIEDIFIDLTNKFGFQRDSMRNMFDHFMTLLDSRSSRMSPEQALLSLHADYIGGDTANYKKWYFAAQLDMDDEIGFRNMKLGKLSRKARKAKKKNKKAMQEASPEDTEETLNQIEGDNSLEAADFRWKSKMNQLSPFEMVRQIALFLLCWGEANQVRFTPECLCFIYKCASDYLDSAQCQQRPDPLPEGDFLNRVITPLYRFIRSQVYEIVDGRYVKSEKDHNKVIGYDDVNQLFWYPEGIAKIVMEDGTRLIDLPAEERYLKLGEIPWDDVFFKTYKETRSWLHLVTNFNRIWIMHISVYWMYCAYNAPTFYTHNYQQLVDNQPLAAYKWATAALGGTVASLIQVAATLCEWSFVPRKWAGAQHLSRRFWFLCVIMGINLGPVIFVFAYDKDTVYSTAAHVVGAVMFFVAVATLVFFSVMPLGGLFTSYMKKSTRSYVASQTFTASFAPLHGLDRWMSYLVWVTVFAAKYAESYFFLILSLRDPIRILSTTSMRCTGEYWWGNKICKVQPKIVLGLMIATDFILFFLDTYLWYIVVNTVFSVGKSFYLGISILTPWRNIFTRLPKRIYSKILATTDMEIKYKPKVLISQIWNAIIISMYREHLLAIDHVQKLLYHQVPSEIEGKRTLRAPTFFVSQDDNNFETEFFPRDSEAERRISFFAQSLSTPIPEPLPVDNMPTFTVLTPHYAERILLSLREIIREDDQFSRVTLLEYLKQLHPVEWDCFVKDTKILAEETAAYENNEDEPEKEDALKSQIDDLPFYCIGFKSAAPEYTLRTRIWASLRSQTLYRTISGFMNYSRAIKLLYRVENPEIVQMFGGNADGLERELEKMARRKFKFLVSMQRLAKFKPHELENAEFLLRAYPDLQIAYLDEEPPLNEGEEPRIYSALIDGHCEILENGRRRPKFRVQLSGNPILGDGKSDNQNHALIFYRGEYIQLIDANQDNYLEECLKIRSVLAEFEELGIEQIHPYTPGLKYEDQSTNHPVAIVGAREYIFSENSGVLGDVAAGKEQTFGTLFARTLAQIGGKLHYGHPDFINATFMTTRGGVSKAQKGLHLNEDIYAGMNAVLRGGRIKHCEYYQCGKGRDLGFGTILNFTTKIGAGMGEQMLSREYYYLGTQLPIDRFLTFYYAHPGFHLNNLFIQLSLQMFMLTLVNLHALAHESILCVYDRDKPITDVLYPIGCYNFHPAIDWVRRYTLSIFIVFWIAFVPIVVQELIERGLWKATQRFFRHILSLSPMFEVFAGQIYSSALLSDIAVGGARYISTGRGFATSRIPFSILYSRFAGSAIYMGSRSMLMLLFGTVAHWQAPLLWFWASLSALIFAPFIFNPHQFAWEDFFLDYRDYIRWLSRGNNKYHRNSWIGYVRMSRSRVTGFKRKLVGDESEKSAGDASRAHRTNLIMAEIIPCAIYAAGCFIAFTFINAQTGVKTTDEDRVNSTLRIIICTLAPIVIDIGVLFFCMGLSCCSGPLLGMCCKKTGSVMAGIAHGIAVVVHIVFFIVMWVLEGFSFVRMLIGVVTCIQCQRLIFHCMTVLLLTREFKNDHANTAFWTGKWYSTGLGYMAWTQPTRELTAKVIELSEFAADFVLGHVILIFQLPVICIPKIDKFHSIMLFWLKPSRQIRPPIYSLKQARLRKRMVRRYCSLYFLVLIIFAGCIVGPAVASAHVPKDLGSGLTGTFHNLVQPRNVSNNDTGSQMSTYKSHYYTHTPSLKTWSTIK

Target ORF information:

RefSeq Version	NM_001181161.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C 1,3-beta-glucan synthase GSC2 (GSC2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181161.3

ORF Insert Sequence:

ATGTCCTACA ACGATCCAAA CTTGAATGGA CAGTATTACA GTAACGGTGA TGGGACTGGT 
GACGGTAATT ACCCTACGTA CCAAGTGACA CAGGATCAAA GTGCGTACGA TGAGTACGGT 
CAGCCAATCT ATACACAAAA CCAACTGGAT GATGGTTATT ATGATCCAAA CGAACAATAC 
GTTGACGGTA CACAATTTCC TCAGGGACAA GATCCTTCAC AAGACCAAGG TCCTTATAAT 
AACGATGCTA GTTACTATAA CCAACCCCCC AATATGATGA ACCCGTCTTC TCAAGATGGA 
GAGAACTTCT CAGATTTTAG CAGCTATGGT CCCCCATCCG GCACTTATCC TAACGATCAA 
TATACTCCTT CTCAAATGAG TTATCCTGAT CAAGATGGTT CTTCAGGGGC CTCAACCCCC 
TATGGAAATG GTGTCGTTAA TGGTAATGGC CAGTACTACG ACCCTAATGC TATTGAAATG 
GCTTTACCAA ATGATCCATA TCCCGCATGG ACCGCAGATC CCCAGTCTCC CCTGCCCATC 
GAACAAATCG AAGATATCTT CATAGATTTA ACAAATAAAT TCGGTTTTCA GAGAGATTCC 
ATGAGAAATA TGTTTGACCA TTTTATGACC CTTTTGGACT CTAGATCTTC TAGGATGTCT 
CCAGAACAGG CCCTTTTATC ATTACATGCA GACTACATAG GTGGAGATAC GGCCAACTAC 
AAAAAATGGT ACTTTGCCGC TCAACTTGAT ATGGATGATG AAATTGGTTT CAGGAATATG 
AAGTTGGGTA AGCTATCAAG AAAGGCAAGA AAGGCTAAGA AGAAAAATAA AAAAGCCATG 
CAAGAGGCTA GTCCTGAAGA CACTGAGGAG ACTTTAAATC AAATTGAGGG TGATAACTCA 
TTAGAAGCTG CGGATTTTAG ATGGAAGTCA AAGATGAATC AACTTTCTCC ATTTGAAATG 
GTTCGTCAAA TTGCCTTGTT TTTATTATGT TGGGGCGAGG CAAATCAAGT CAGATTTACC 
CCGGAGTGTC TTTGTTTCAT TTATAAATGC GCCTCTGATT ACTTAGATTC TGCACAATGT 
CAACAACGTC CTGATCCCTT GCCTGAAGGT GATTTTTTGA ATAGAGTTAT TACTCCTCTT 
TATCGTTTTA TTAGGAGCCA GGTTTACGAA ATCGTGGATG GTCGATACGT GAAGAGTGAA 
AAAGATCATA ACAAAGTTAT TGGGTATGAT GATGTCAATC AATTATTCTG GTATCCAGAA 
GGTATAGCAA AAATTGTCAT GGAAGATGGA ACCAGGTTGA TTGATTTGCC AGCAGAGGAG 
CGTTATTTGA AATTGGGAGA AATTCCCTGG GATGATGTCT TCTTTAAAAC TTACAAAGAA 
ACACGTTCCT GGTTACATTT AGTTACCAAC TTCAATCGTA TTTGGATCAT GCACATCTCA 
GTATATTGGA TGTATTGTGC TTACAATGCT CCAACTTTTT ATACTCACAA CTATCAACAA 
TTGGTCGACA ATCAGCCTTT GGCAGCTTAT AAATGGGCCA CTGCAGCATT AGGTGGTACT 
GTGGCAAGTT TGATTCAAGT TGCCGCTACT TTGTGCGAGT GGTCATTCGT TCCTAGAAAA 
TGGGCGGGTG CTCAACATTT GTCCCGTAGA TTCTGGTTCT TGTGTGTCAT TATGGGTATT 
AATTTGGGGC CTGTGATATT TGTTTTCGCT TATGATAAGG ACACAGTATA TTCTACTGCC 
GCTCATGTCG TTGGAGCAGT TATGTTTTTT GTTGCTGTGG CAACACTTGT TTTCTTTTCC 
GTAATGCCAT TGGGTGGATT ATTTACATCG TATATGAAAA AGTCCACAAG AAGTTATGTT 
GCCTCACAGA CCTTCACCGC ATCTTTTGCT CCATTGCATG GTTTAGACAG GTGGATGTCT 
TATTTGGTTT GGGTAACCGT TTTTGCTGCT AAATATGCAG AGTCATATTT TTTTCTAATA 
CTGTCACTAA GAGATCCAAT TAGGATTTTA TCTACTACAT CAATGAGATG TACTGGTGAA 
TACTGGTGGG GTAATAAGAT TTGTAAGGTC CAGCCAAAGA TTGTTTTAGG TTTAATGATT 
GCGACTGACT TCATTTTGTT CTTTTTGGAT ACCTACTTGT GGTATATCGT TGTTAACACT 
GTTTTCTCGG TCGGAAAATC GTTCTATTTG GGTATTTCTA TCTTAACTCC ATGGAGAAAT 
ATTTTCACTA GATTGCCAAA AAGAATTTAT TCTAAGATCT TGGCTACTAC TGATATGGAA 
ATAAAATATA AACCGAAAGT ACTAATTTCT CAGATTTGGA ATGCTATCAT TATCTCCATG 
TACAGAGAAC ATTTATTAGC CATAGACCAT GTACAAAAAT TGTTATATCA TCAGGTTCCG 
TCCGAAATTG AAGGTAAGAG GACTTTGAGA GCACCAACTT TCTTTGTTTC CCAAGATGAC 
AATAATTTTG AGACTGAATT TTTCCCTAGA GATTCAGAAG CTGAGCGCCG TATTTCATTT 
TTTGCCCAAT CTCTATCCAC TCCAATTCCA GAACCACTAC CAGTTGACAA CATGCCAACA 
TTTACTGTAT TAACTCCCCA TTACGCCGAG AGGATTCTAT TATCATTGAG AGAAATTATT 
CGTGAAGATG ATCAATTTTC AAGAGTTACT CTTTTGGAAT ACCTGAAGCA ATTACACCCG 
GTAGAATGGG ACTGTTTTGT TAAGGATACG AAAATTCTTG CTGAAGAAAC GGCCGCATAT 
GAAAACAATG AAGATGAACC TGAAAAGGAA GACGCTCTGA AATCTCAAAT TGATGATTTA 
CCTTTCTATT GTATTGGTTT CAAATCTGCT GCACCAGAAT ACACCTTACG TACGAGAATC 
TGGGCCTCTT TAAGGTCGCA AACTTTGTAT CGCACAATCT CGGGGTTTAT GAATTATTCG 
AGGGCCATAA AATTACTTTA TCGTGTGGAA AATCCAGAAA TCGTTCAAAT GTTCGGTGGT 
AATGCTGATG GATTAGAAAG AGAACTGGAA AAAATGGCAA GGCGAAAATT CAAATTCTTG 
GTTTCGATGC AAAGATTGGC CAAGTTTAAA CCACATGAAC TAGAAAATGC TGAGTTCCTG 
TTGAGAGCTT ATCCGGACTT GCAAATTGCC TACCTGGATG AAGAACCTCC CTTAAACGAA 
GGCGAAGAGC CAAGAATTTA CTCGGCCTTA ATTGATGGTC ATTGTGAGAT TTTAGAGAAT 
GGTCGTAGAC GTCCCAAATT TAGAGTTCAA CTATCCGGTA ATCCAATTCT TGGTGATGGT 
AAATCAGATA ATCAAAATCA TGCTTTGATT TTTTACAGAG GTGAGTATAT TCAATTGATT 
GATGCTAATC AAGACAATTA CTTGGAAGAG TGTTTGAAAA TCAGGTCTGT CTTAGCAGAA 
TTTGAAGAAT TGGGAATTGA GCAAATTCAT CCTTATACTC CTGGTTTAAA ATATGAGGAC 
CAATCCACAA ATCATCCTGT TGCAATTGTC GGCGCTAGAG AATATATTTT CTCAGAAAAC 
TCTGGTGTTC TTGGTGATGT AGCGGCTGGT AAAGAACAAA CTTTTGGTAC ATTATTTGCC 
CGTACTTTGG CACAGATTGG TGGTAAATTG CATTATGGTC ATCCAGATTT TATTAATGCG 
ACATTCATGA CTACTAGGGG TGGTGTTTCC AAAGCACAAA AGGGTCTACA TTTAAATGAA 
GATATTTATG CCGGTATGAA TGCCGTACTT CGGGGTGGTC GTATCAAGCA TTGCGAATAT 
TATCAGTGTG GTAAAGGTAG AGATTTAGGT TTTGGTACAA TTTTGAATTT CACTACTAAG 
ATCGGTGCTG GTATGGGTGA ACAAATGTTA TCTCGTGAAT ACTATTATTT GGGTACGCAA 
TTACCTATTG ACCGTTTTTT AACATTTTAT TATGCGCATC CAGGGTTTCA CTTGAATAAC 
TTATTTATTC AATTGTCTCT GCAGATGTTC ATGTTAACTT TAGTGAACTT GCATGCTTTG 
GCTCATGAAT CCATTCTGTG TGTTTACGAT AGGGATAAGC CAATTACTGA TGTTTTGTAT 
CCAATTGGTT GTTACAACTT TCATCCTGCG ATTGATTGGG TGAGACGTTA TACACTCTCT 
ATTTTCATCG TCTTTTGGAT TGCTTTTGTC CCTATTGTCG TTCAGGAATT AATCGAGCGT 
GGTCTGTGGA AGGCGACACA AAGATTTTTC CGTCACATTT TATCTCTATC TCCAATGTTT 
GAAGTCTTTG CTGGCCAAAT CTATTCTTCA GCACTGTTAA GTGATATCGC TGTGGGTGGT 
GCTCGTTATA TTTCAACAGG TCGTGGCTTT GCTACATCTC GTATACCTTT CTCTATTCTT 
TATTCAAGAT TTGCGGGTTC AGCCATTTAT ATGGGATCAA GATCAATGTT GATGTTATTA 
TTTGGTACCG TGGCACATTG GCAAGCTCCA CTATTATGGT TTTGGGCATC ATTATCAGCC 
TTAATCTTTG CACCATTCAT TTTCAATCCA CATCAATTTG CTTGGGAAGA TTTTTTCCTA 
GACTACAGAG ATTATATCAG ATGGCTGTCA AGAGGTAATA ATAAGTACCA CAGGAACTCA 
TGGATTGGTT ATGTAAGAAT GTCGAGGTCT CGTGTTACTG GTTTCAAGCG CAAACTGGTG 
GGTGATGAGT CTGAAAAATC TGCAGGCGAT GCAAGCAGGG CTCATAGAAC CAATTTAATT 
ATGGCTGAAA TTATACCGTG TGCGATTTAC GCAGCAGGTT GTTTTATTGC CTTCACGTTT 
ATTAATGCAC AAACTGGTGT CAAGACTACT GATGAAGATA GAGTAAATTC CACCTTACGT 
ATCATCATTT GCACCTTGGC GCCTATTGTT ATCGATATTG GTGTTTTATT CTTCTGTATG 
GGTTTGTCAT GCTGCTCTGG CCCTTTGTTG GGCATGTGCT GCAAGAAAAC TGGTTCTGTT 
ATGGCAGGGA TCGCTCACGG TATCGCTGTT GTTGTCCATA TTGTCTTTTT CATTGTCATG 
TGGGTTTTAG AGGGTTTTAG TTTTGTTAGG ATGTTGATTG GCGTTGTTAC ATGTATACAA 
TGTCAAAGGT TGATTTTTCA CTGTATGACT GTACTGTTGC TGACCCGTGA GTTCAAGAAT 
GATCACGCTA ATACTGCCTT TTGGACAGGC AAATGGTACA GCACCGGTTT AGGATATATG 
GCATGGACTC AACCGACAAG GGAATTGACT GCAAAAGTCA TTGAGCTTTC CGAGTTTGCA 
GCGGATTTTG TTTTGGGGCA TGTAATTTTG ATCTTCCAAC TACCAGTCAT TTGTATTCCA 
AAGATAGATA AGTTTCACTC CATCATGTTA TTTTGGTTAA AACCATCCCG TCAAATCCGT 
CCTCCTATTT ACTCTTTGAA ACAAGCACGC CTACGTAAAC GTATGGTTAG GAGGTATTGC 
AGCTTGTACT TTTTGGTACT GATCATATTC GCGGGATGCA TCGTTGGCCC TGCCGTTGCT 
TCAGCACATG TTCCAAAAGA CCTTGGATCT GGGTTGACGG GTACTTTCCA TAACTTGGTT 
CAACCAAGGA ACGTATCTAA CAATGATACA GGGTCCCAGA TGTCTACTTA TAAGAGTCAT 
TATTACACTC ATACGCCATC CTTAAAGACC TGGTCTACGA TCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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GSC2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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