KAP114 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	KAP114
Entrez Gene ID	852610
Full Name	Kap114p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181107.1	NP_011273.1	Kap114p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	KAP114	NP_011273.1
Eremothecium gossypii	AGOS_AFR269W	NP_985816.3
Neurospora crassa	NCU06578	XP_961938.1
Schizosaccharomyces pombe (fission yeast)	kap114	NP_593739.2
Magnaporthe oryzae (rice blast fungus)	MGG_11165	XP_003714685.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It is proposed that sumoylation occurs at the site of Kap114 cargo function and that small ubiquitin-related modifier is a cargo release factor involved in intranuclear targeting.
Title: Sumoylation regulates Kap114-mediated nuclear transport.

TFIIB nuclear import is mediated by Kap114p
Title: Nuclear import of TFIIB is mediated by Kap114p, a karyopherin with multiple cargo-binding domains.

Kap114p, responsible for the nuclear import of histones H2A and H2B, modulates the deposition of histones H2A and H2B by the histone chaperone Nap1p.
Title: Modulation of histone deposition by the karyopherin kap114.

The KAP114 protein functions is in a parallel import pathway for H2A and H2B.
Title: The histones H2A/H2B and H3/H4 are imported into the yeast nucleus by different mechanisms.

The following KAP114 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KAP114 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02000	NM_001181107.1 Latest version!	Saccharomyces cerevisiae S288C Kap114p (KAP114), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KAP114 ( NM_001181107.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011273.1 Saccharomyces cerevisiae S288C Kap114p (KAP114), partial mRNA.

Price & Availability↑

CloneID	OSi02000
Clone ID Related Accession (Same CDS sequence)	NM_001181107.1
Accession Version	NM_001181107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Kap114p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATATTA ATGAATTGAT AATAGGTGCT CAATCAGCTG ATAAACACAC TAGAGAAGTT 
GCGGAAACGC AATTGTTACA GTGGTGTGAT TCCGATGCAT CTCAAGTGTT TAAAGCGCTG 
GCAAACGTTG CCTTACAGCA CGAAGCTTCT CTAGAATCGA GACAGTTTGC TTTATTGTCG 
TTAAGAAAAT TGATCACCAT GTATTGGAGT CCAGGATTTG AATCATACCG TTCTACATCG 
AACGTCGAAA TAGATGTTAA AGATTTTATC CGAGAAGTGC TACTGAAATT ATGCTTAAAT 
GACAATGAAA ATACCAAAAT AAAAAATGGT GCATCTTACT GTATTGTTCA GATATCTGCG 
GTTGATTTTC CTGATCAATG GCCGCAGCTT TTAACAGTTA TTTATGATGC TATCTCTCAT 
CAGCATTCAT TAAATGCGAT GTCTTTATTG AATGAAATTT ACGATGATGT CGTATCTGAA 
GAAATGTTCT TTGAAGGCGG AATTGGCTTG GCGACAATGG AGATCGTTTT CAAAGTACTC 
AATACAGAAA CTTCTACTTT GATAGCCAAA ATTGCTGCTT TGAAGCTACT TAAAGCATGT 
CTTCTACAAA TGAGCTCCCA TAATGAGTAT GATGAGGCGT CACGAAAAAG TTTTGTCTCC 
CAATGTTTAG CTACTTCTTT ACAGATATTG GGACAATTGT TGACTTTGAA TTTTGGAAAC 
GTAGATGTTA TTTCTCAGTT GAAATTTAAA TCCATTATAT ACGAGAATTT GGTTTTTATT 
AAAAATGATT TCTCTAGAAA GCATTTCTCA AGTGAATTAC AAAAACAGTT CAAAATAATG 
GCAATCCAAG ATCTGGAAAA CGTTACCCAT ATAAATGCAA ATGTCGAAAC TACTGAAAGT 
GAACCCCTTT TAGAAACAGT TCATGATTGT TCTATTTATA TTGTAGAATT TTTGACTAGT 
GTTTGCACTT TGCAATTTAG TGTTGAAGAG ATGAACAAAA TTATAACATC TTTAACCATT 
CTTTGTCAGC TAAGTTCTGA GACAAGAGAG ATATGGACAA GCGATTTCAA TACATTTGTA 
TCGAAGGAAA CAGGCCTAGC AGCATCATAT AATGTTAGAG ATCAAGCCAA TGAATTTTTT 
ACTTCATTAC CCAATCCTCA ACTTTCCCTA ATTTTCAAAG TTGTTTCCAA CGATATAGAA 
CACAGCACGT GTAATTATTC AACTTTAGAA TCTTTACTAT ATCTCTTACA GTGTATTTTG 
CTTAATGATG ATGAGATAAC TGGTGAAAAT ATCGATCAAT CTTTGCAAAT TTTAATAAAA 
ACGTTAGAAA ATATCTTAGT ATCACAAGAA ATACCAGAAT TAATATTGGC GCGTGCAATT 
TTGACTATTC CCAGAGTACT AGACAAATTT ATAGATGCAT TACCTGATAT CAAACCCTTG 
ACATCTGCAT TCTTGGCAAA AAGTCTTAAT TTGGCGCTAA AATCTGATAA AGAGCTCATT 
AAATCCGCAA CATTAATAGC ATTTACGTAC TATTGTTATT TTGCCGAACT AGATTCAGTC 
CTTGGACCAG AGGTCTGCTC AGAAACCCAG GAAAAAGTAA TTCGAATAAT CAACCAGGTT 
AGTAGTGATG CCGAAGAGGA CACTAATGGG GCTTTAATGG AAGTATTAAG TCAAGTCATT 
AGCTACAATC CGAAAGAACC TCATTCAAGA AAGGAAATTT TACAGGCTGA ATTTCATCTT 
GTGTTCACGA TCTCATCCGA GGATCCAGCA AATGTACAGG TCGTTGTACA AAGTCAAGAA 
TGCTTGGAGA AACTATTGGA TAATATCAAT ATGGATAATT ACAAAAATTA CATTGAATTA 
TGTCTCCCAT CATTTATTAA TGTGCTAGAT TCCAACAATG CAAACAATTA TAGATACTCA 
CCCCTGCTAT CATTAGTCTT GGAATTCATC ACAGTTTTTT TGAAGAAAAA ACCCAACGAT 
GGATTTTTAC CAGATGAAAT AAATCAATAC CTATTTGAAC CATTAGCAAA AGTATTAGCG 
TTCTCTACTG AAGATGAGAC CCTACAGTTA GCCACGGAGG CATTCAGCTA CCTGATTTTC 
AACACAGATA CACGAGCCAT GGAGCCAAGG TTAATGGATA TCATGAAGGT TCTAGAAAGG 
CTGCTGTCTT TGGAGGTATC GGATTCAGCC GCTATGAACG TCGGTCCATT GGTAGTTGCC 
ATATTCACAA GGTTTTCAAA GGAAATCCAA CCGTTAATAG GAAGAATTTT GGAAGCCGTT 
GTGGTAAGGT TAATTAAGAC ACAAAACATT TCTACTGAGC AAAACCTTCT TTCTGTATTA 
TGCTTCTTAA CGTGTAATGA TCCTAAACAA ACGGTGGATT TTTTGTCTAG TTTCCAAATT 
GACAATACTG ATGCTTTGAC ATTGGTGATG CGAAAATGGA TAGAAGCCTT TGAAGTCATT 
AGAGGTGAAA AAAGAATCAA AGAAAATATC GTTGCTTTGA GCAACCTTTT CTTCCTGAAC 
GACAAAAGGC TACAGAAAGT GGTTGTCAAT GGTAACTTGA TACCATACGA AGGGGACCTG 
ATCATTACAA GATCAATGGC TAAGAAAATG CCAGACAGGT ATGTACAAGT TCCACTTTAT 
ACCAAGATCA TTAAATTGTT TGTTTCTGAG TTAAGCTTCC AAAGTAAACA ACCGAATCCA 
GAACAATTGA TCACAAGCGA TATTAAGCAA GAAGTGGTAA ATGCTAATAA AGATGACGAC 
AATGATGATT GGGAAGATGT AGATGATGTA CTCGATTACG ATAAGTTGAA AGAATATATT 
GATGATGATG TCGATGAAGA AGCAGACGAC GATTCAGACG ATATAACGGG ACTCATGGAC 
GTGAAGGAAT CTGTTGTTCA GCTACTGGTA AGATTCTTTA AAGAGGTTGC AAGCAAAGAT 
GTGAGTGGCT TCCATTGTAT TTACGAGACT CTATCCGACA GTGAACGTAA AGTTCTTTCA 
GAAGCCCTTT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011273.1
CDS	1..3015
Translation	MDINELIIGAQSADKHTREVAETQLLQWCDSDASQVFKALANVALQHEASLESRQFALLSLRKLITMYWSPGFESYRSTSNVEIDVKDFIREVLLKLCLNDNENTKIKNGASYCIVQISAVDFPDQWPQLLTVIYDAISHQHSLNAMSLLNEIYDDVVSEEMFFEGGIGLATMEIVFKVLNTETSTLIAKIAALKLLKACLLQMSSHNEYDEASRKSFVSQCLATSLQILGQLLTLNFGNVDVISQLKFKSIIYENLVFIKNDFSRKHFSSELQKQFKIMAIQDLENVTHINANVETTESEPLLETVHDCSIYIVEFLTSVCTLQFSVEEMNKIITSLTILCQLSSETREIWTSDFNTFVSKETGLAASYNVRDQANEFFTSLPNPQLSLIFKVVSNDIEHSTCNYSTLESLLYLLQCILLNDDEITGENIDQSLQILIKTLENILVSQEIPELILARAILTIPRVLDKFIDALPDIKPLTSAFLAKSLNLALKSDKELIKSATLIAFTYYCYFAELDSVLGPEVCSETQEKVIRIINQVSSDAEEDTNGALMEVLSQVISYNPKEPHSRKEILQAEFHLVFTISSEDPANVQVVVQSQECLEKLLDNINMDNYKNYIELCLPSFINVLDSNNANNYRYSPLLSLVLEFITVFLKKKPNDGFLPDEINQYLFEPLAKVLAFSTEDETLQLATEAFSYLIFNTDTRAMEPRLMDIMKVLERLLSLEVSDSAAMNVGPLVVAIFTRFSKEIQPLIGRILEAVVVRLIKTQNISTEQNLLSVLCFLTCNDPKQTVDFLSSFQIDNTDALTLVMRKWIEAFEVIRGEKRIKENIVALSNLFFLNDKRLQKVVVNGNLIPYEGDLIITRSMAKKMPDRYVQVPLYTKIIKLFVSELSFQSKQPNPEQLITSDIKQEVVNANKDDDNDDWEDVDDVLDYDKLKEYIDDDVDEEADDDSDDITGLMDVKESVVQLLVRFFKEVASKDVSGFHCIYETLSDSERKVLSEALL

Target ORF information:

RefSeq Version	NM_001181107.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Kap114p (KAP114), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181107.1

ORF Insert Sequence:

ATGGATATTA ATGAATTGAT AATAGGTGCT CAATCAGCTG ATAAACACAC TAGAGAAGTT 
GCGGAAACGC AATTGTTACA GTGGTGTGAT TCCGATGCAT CTCAAGTGTT TAAAGCGCTG 
GCAAACGTTG CCTTACAGCA CGAAGCTTCT CTAGAATCGA GACAGTTTGC TTTATTGTCG 
TTAAGAAAAT TGATCACCAT GTATTGGAGT CCAGGATTTG AATCATACCG TTCTACATCG 
AACGTCGAAA TAGATGTTAA AGATTTTATC CGAGAAGTGC TACTGAAATT ATGCTTAAAT 
GACAATGAAA ATACCAAAAT AAAAAATGGT GCATCTTACT GTATTGTTCA GATATCTGCG 
GTTGATTTTC CTGATCAATG GCCGCAGCTT TTAACAGTTA TTTATGATGC TATCTCTCAT 
CAGCATTCAT TAAATGCGAT GTCTTTATTG AATGAAATTT ACGATGATGT CGTATCTGAA 
GAAATGTTCT TTGAAGGCGG AATTGGCTTG GCGACAATGG AGATCGTTTT CAAAGTACTC 
AATACAGAAA CTTCTACTTT GATAGCCAAA ATTGCTGCTT TGAAGCTACT TAAAGCATGT 
CTTCTACAAA TGAGCTCCCA TAATGAGTAT GATGAGGCGT CACGAAAAAG TTTTGTCTCC 
CAATGTTTAG CTACTTCTTT ACAGATATTG GGACAATTGT TGACTTTGAA TTTTGGAAAC 
GTAGATGTTA TTTCTCAGTT GAAATTTAAA TCCATTATAT ACGAGAATTT GGTTTTTATT 
AAAAATGATT TCTCTAGAAA GCATTTCTCA AGTGAATTAC AAAAACAGTT CAAAATAATG 
GCAATCCAAG ATCTGGAAAA CGTTACCCAT ATAAATGCAA ATGTCGAAAC TACTGAAAGT 
GAACCCCTTT TAGAAACAGT TCATGATTGT TCTATTTATA TTGTAGAATT TTTGACTAGT 
GTTTGCACTT TGCAATTTAG TGTTGAAGAG ATGAACAAAA TTATAACATC TTTAACCATT 
CTTTGTCAGC TAAGTTCTGA GACAAGAGAG ATATGGACAA GCGATTTCAA TACATTTGTA 
TCGAAGGAAA CAGGCCTAGC AGCATCATAT AATGTTAGAG ATCAAGCCAA TGAATTTTTT 
ACTTCATTAC CCAATCCTCA ACTTTCCCTA ATTTTCAAAG TTGTTTCCAA CGATATAGAA 
CACAGCACGT GTAATTATTC AACTTTAGAA TCTTTACTAT ATCTCTTACA GTGTATTTTG 
CTTAATGATG ATGAGATAAC TGGTGAAAAT ATCGATCAAT CTTTGCAAAT TTTAATAAAA 
ACGTTAGAAA ATATCTTAGT ATCACAAGAA ATACCAGAAT TAATATTGGC GCGTGCAATT 
TTGACTATTC CCAGAGTACT AGACAAATTT ATAGATGCAT TACCTGATAT CAAACCCTTG 
ACATCTGCAT TCTTGGCAAA AAGTCTTAAT TTGGCGCTAA AATCTGATAA AGAGCTCATT 
AAATCCGCAA CATTAATAGC ATTTACGTAC TATTGTTATT TTGCCGAACT AGATTCAGTC 
CTTGGACCAG AGGTCTGCTC AGAAACCCAG GAAAAAGTAA TTCGAATAAT CAACCAGGTT 
AGTAGTGATG CCGAAGAGGA CACTAATGGG GCTTTAATGG AAGTATTAAG TCAAGTCATT 
AGCTACAATC CGAAAGAACC TCATTCAAGA AAGGAAATTT TACAGGCTGA ATTTCATCTT 
GTGTTCACGA TCTCATCCGA GGATCCAGCA AATGTACAGG TCGTTGTACA AAGTCAAGAA 
TGCTTGGAGA AACTATTGGA TAATATCAAT ATGGATAATT ACAAAAATTA CATTGAATTA 
TGTCTCCCAT CATTTATTAA TGTGCTAGAT TCCAACAATG CAAACAATTA TAGATACTCA 
CCCCTGCTAT CATTAGTCTT GGAATTCATC ACAGTTTTTT TGAAGAAAAA ACCCAACGAT 
GGATTTTTAC CAGATGAAAT AAATCAATAC CTATTTGAAC CATTAGCAAA AGTATTAGCG 
TTCTCTACTG AAGATGAGAC CCTACAGTTA GCCACGGAGG CATTCAGCTA CCTGATTTTC 
AACACAGATA CACGAGCCAT GGAGCCAAGG TTAATGGATA TCATGAAGGT TCTAGAAAGG 
CTGCTGTCTT TGGAGGTATC GGATTCAGCC GCTATGAACG TCGGTCCATT GGTAGTTGCC 
ATATTCACAA GGTTTTCAAA GGAAATCCAA CCGTTAATAG GAAGAATTTT GGAAGCCGTT 
GTGGTAAGGT TAATTAAGAC ACAAAACATT TCTACTGAGC AAAACCTTCT TTCTGTATTA 
TGCTTCTTAA CGTGTAATGA TCCTAAACAA ACGGTGGATT TTTTGTCTAG TTTCCAAATT 
GACAATACTG ATGCTTTGAC ATTGGTGATG CGAAAATGGA TAGAAGCCTT TGAAGTCATT 
AGAGGTGAAA AAAGAATCAA AGAAAATATC GTTGCTTTGA GCAACCTTTT CTTCCTGAAC 
GACAAAAGGC TACAGAAAGT GGTTGTCAAT GGTAACTTGA TACCATACGA AGGGGACCTG 
ATCATTACAA GATCAATGGC TAAGAAAATG CCAGACAGGT ATGTACAAGT TCCACTTTAT 
ACCAAGATCA TTAAATTGTT TGTTTCTGAG TTAAGCTTCC AAAGTAAACA ACCGAATCCA 
GAACAATTGA TCACAAGCGA TATTAAGCAA GAAGTGGTAA ATGCTAATAA AGATGACGAC 
AATGATGATT GGGAAGATGT AGATGATGTA CTCGATTACG ATAAGTTGAA AGAATATATT 
GATGATGATG TCGATGAAGA AGCAGACGAC GATTCAGACG ATATAACGGG ACTCATGGAC 
GTGAAGGAAT CTGTTGTTCA GCTACTGGTA AGATTCTTTA AAGAGGTTGC AAGCAAAGAT 
GTGAGTGGCT TCCATTGTAT TTACGAGACT CTATCCGACA GTGAACGTAA AGTTCTTTCA 
GAAGCCCTTT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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KAP114 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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