TOM1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TOM1
Entrez Gene ID	852068
Full Name	E3 ubiquitin-protein ligase TOM1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180765.3	NP_010745.3	E3 ubiquitin-protein ligase TOM1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B01804g	XP_451611.1
Eremothecium gossypii	AGOS_AER304C	NP_985161.2
Schizosaccharomyces pombe (fission yeast)	ptr1	NP_594902.1
Saccharomyces cerevisiae (baker's yeast)	TOM1	NP_010745.3

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Together, these data point to an important role for Tom1, Ubc4 and Ubc5 in normal physiology and prompt the authors to refer to this pathway as ERISQ, for excess ribosomal protein quality control.
Title: A conserved quality-control pathway that mediates degradation of unassembled ribosomal proteins.

Tom1 and the Dia2 control Cdc6 degradation in G1 phase.
Title: The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase.

Data demonstrated that excess histones associate with Rad53, and also identified Ubc4 and Ubc5, as well as Tom1 as enzymes involved in the ubiquitylation of excess histones.
Title: Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis.

The following TOM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01527	NM_001180765.3 Latest version!	Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase TOM1 (TOM1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOM1 ( NM_001180765.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010745.3 Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase TOM1 (TOM1), partial mRNA.

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CloneID	OSi01527
Clone ID Related Accession (Same CDS sequence)	NM_001180765.3
Accession Version	NM_001180765.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	E3 ubiquitin-protein ligase TOM1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180765.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTGCTTT TTACTCGGTG TGAAAAGGCA AGAAAGGAGA AACTCGCCGC TGGTTATAAG 
CCCTTAGTTG ATTATTTGAT TGATTGTGAT ACTCCCACAT TTTTAGAAAG AATTGAGGCA 
ATCCAAGAGT GGGACCGATC CAGAGATGAT CTTTATGTTT GGATTCCCAT TCTAGACAGA 
ATGGACGGGC TGTTGTTAAA AGTGGCAGAG AAATATAAAT ACAAGCAAGA CCCCAAGAAG 
GAGTGTGAAG TCAAATTGGT GGAAATGGAA GCGCATGATG TTGATTATTG TTTGAAAATG 
CTAAAGTTTA CTCGTCGACT ACTTCTAAAT ACAGAAAACA GGTTTGTTTA TTCATCTGGA 
GATGTTTTAA TGTATCTACT GAATTGTCCG AATTTTACAA TAAAACTAGC GGTCATGCGA 
ATTTTAGCCA TTTTAGGGGA AAGGTTTGTG ATTGCGAGAG AAAAAATAGT TGCACATAAC 
ATATTTGGAG ATCACAACCT CAGGAAAAAA ACTCTCAAAC TAGCTTTATC TCTGTCATCT 
TCCGTCATGG ATGAAGACGG AGAGCACTTT TCTCTTGTCG ATCTATATTT TGATAAGAAA 
AAAGTGCCTC AAAAATGGAG AAAGTTGAGA TTTACACATT ATACTTCAAA CGACTTTAAA 
AAGTCAAGCC AGCAAAAAAA TAACATTAAT GAAACCCAAA CTTCCATTAA GAAGGTAACT 
ATGACGACCC AAGAACTGTG TGAACACTCA CTACAGCAAA TATTTGATAA GGGTATGGCC 
CTTTTACCCG CAGAAAGTTG GTTTGATTTC TCAATAAAAG CTTCGGTGGC GAAGGCCTTC 
AGTGATGATT CAGGTGAAAA TATAGATTTG AGAAATATAA TTATTGAAAC AAAACTGAAT 
GCTATTGCAT TTGTTAACAC CATTTTCTCG CCTCCTCAAG TAAGCTCGAA GTTATTTGAA 
TTAGATCCGT ATGCTTTTAA TAGTCTAACT GATTTGATAT CGTTGTCTGA AACCAAAATA 
CCAAAAGAGC TAAGAACGGA TGCTTTATTC ACATTGGAAT GTATATCCTT GAAGCATGTC 
TGGTGCTCAG ACATTATCAG AAATCTCGGT GGTAACATAT CGCATGGTCT GTTGTTTCAG 
ATTCTCCGTT ACATTGCCAA GACATTGAGA GAAGCGACAG ACGAAATCGA TGAAGAATAT 
AATGTGAGGT TCTTTTACTT GATATCAAAT TTAGCTGACG TTAAGCCTTT GCATGAATCT 
CTTTTCGCCG CGGGTTTAAT ACCTACTTTA TTGGAAATTG TATCTATAAG AAATTGCCCA 
TATAAGCGTA CCCTCGCATC TGCTACCCAT CTCCTAGAAA CATTTATAGA TAACAGTGAA 
ACTACCACTG AATTTATCGA AAATGATGGG TTTACTATGC TGATCACTTC AGTCGCCAAT 
GAGATTGATT TCACGCTTGC ACATCCAGAG ACGTGGCAAC CGCCAAAATA TTCAGTGGTT 
TATTATTCAA TTTCTTTTAG GGAGTTGGCA TATATTAGAA GTTTATTGAA ACTCGTTCTC 
AAATTATTAA GCACAGACTC AGGGGATAGA ATTAGAAATT TAATAGATTC GCCGATACTT 
GTTTCATTAA AGAAAATCCT AGAAAATAAA CTTGTATTCG GTTTAACATT AATAACTTAC 
ACTCTGGATG TAGTTCAGAA AGTAATAAAT AGTGAGCCTA CTATTTATCC TGTTCTGGTT 
GAAGCAGGAC TTATTCCATA TGTTATCGAT AATTTTCCAA AGTTAATTGG GCCTTCCGCC 
GAATTATTAA GTTTACTTCC AGATGTCGTT TCTGCCATAT GCTTGAATCC AGAAGGGCTG 
AAGCAAGTTA AAGAAAAGGG TCTCATAAAC AATTTGTTTG ATTTCTTACT CGATGCTGAC 
CACGCGCGTA TATTAACAGG TGGAGATCGT TCTACGGAAT ATGGTACTGA CATTGATGAA 
TTAGCAAGAC ATTATCCAGA CTTAAAGGCA AATATCGTGG AGGCTTTGTG TAACGTCATC 
AGAAAAATGC CAAGTACCTT TAGAAATGAA AGAGAGTTTC TTTTTACTTC ACCAAAAGAT 
CAAAAGTACT TTTTTCACAG AAAAAATGAG GAAATATTGA CAGATAAAGA GGAACATGAA 
CCTGCATACT GGGAGCTATT AGATAAGGGA ACTATGCTGG ACACATTTAC CAGTGTTTTG 
TTTGGTATGT CTTTAGGAAA TGGTTCTTTT TCACAGGTGC CACAACATCT GGAAGCGAGA 
GATTTTCTGG CAATAATATT TATGGAAAAT CCTCCTTATG AATACTTTAC CTCCGTGGCA 
ATCTCAAATG TTACTGAGGT CCTCCAGTAT CTGGATGAAA AATACGAAGA CTACGCATTC 
ATGGATGTTA TGAAAGTATT GAACGATCAA TTGGAGAACC TGAATGATTT TTTGAATAGT 
CCAAATGATA GAAGTTTCTT TTTAGAAAGA GATGGGGAAA ACTCGGTGCG TTCATGCCAT 
TCAAAACTTT GTCGACTTGC CGCTATTTTG AACATTGTTA CGAATGTATA CATTGACCTC 
ACTACCTTAT CTTGTAAGAG AATCATGCAG ATCTATTCTT ATTTTGACAA AAGAGGATTT 
TCTTTAATTA AAAACTTAAA GTTGCTATTC CAGAAATGTG CGTTGGAGGA AATGTACATA 
CGCCAACATA TGCCAGATTC TGTTATTACA GAAACAATGC CGTTGCCTAT AGTGGATGTT 
TCCGGAGATG GACCACCACT TCAAATTTAT ATCGATGACC CAAAAAAGGG TGATCAAAAG 
GGGAAAATTA CCAGCGTAAA AACGAGAAAT ACTCTTCAAA TGCGAACAAT ACTGTATACA 
TTACAATCAA ATACTGCAAT TCTATTTCGC TGCTTTTTAA GGTTAAGCCA CTCTAGGAAT 
ATGGATCTAG AGCATAAAGA TTTGACGACA GAAGTTCATA TATTTGAGAA CGTTGTTGAA 
AATGTTATTG AAATGTTGAA GGCCACCGAA CTCGAAGGTC ATCTACCGTA TATTCTCGTT 
TTATTAAACT TCAACACCTT TGTGTTTACT ATTCCAAAGG CTTCCCCTAA TTCCACTGAA 
ATATTACAAA CAATACCGGC TTATATTTTC TATCAAAAGG GGGGCTATTT ACTTTATTTG 
CACATTATAA GGGATCTCTT TACGAGAATG ACAAAAATAA AAGATTTATC CTCACTTGAT 
AACATAAACT ACATAGATGA GTCCAACGGT ATCCTAACCT TAAGCTGTTT AATTAATGCT 
CTCACCTTTT ACAACAAATC AATGCAAACT GAAACCATGG AGAATGTCCA AAGTATTGGT 
AAGTATTACG TTTCAATAGA TGACGATTAT AATATTATGA AAGCTTTAAC AGTTCCTATT 
AAGGTAATGG CTCTCGCAAT GATTTTAGAT TTGGATAAAT CGGATAGCCT CTTCAAGACA 
CAGTCCCGTA ATGTGCCTTA CTCAGTTTTC AAGCAACTTC TTAGTATGCT CAAAAATATA 
TTCACTAATG TGAACATCTA TACCAAGGAA TTATATGAAT TGCATTGGGA CCTAATATTC 
CCACCAATTA AAAAAATTAG CTTGTTTGAG CAAGTCGGCA TTCCCGGTGA TGTTGCGGCA 
AACTATTTAA CAGATACTGG TGATGATCTT CCTGCAGATA ACAGTATTGG CCTGTTTTCG 
CCTGAACAAT GGGAAAAATA TAAAAAATTA ATCGGTGAAG ATAAATCCAT CTACTATCCA 
CAACCAATGC AAGCTCAGTA TTACAAAGGA TGTTCCTCAA AAGAATTAGA TGAGCTGAGA 
GATACCTTTT TCAATGACGG TTTACCATCT AGGATCTTCA CTGTACTCCC TTTCTATCCT 
AAACTTGTAA ACGCGTTTGC AAAAACTTTG CTACAAATTT TTACGAAATA CGATGAACCC 
ACTGAAGTGT TTGCTGGTAG AATCTTAGAT CGAATTTTAG AGACAGATCT AGATGACCCT 
GCTACTTTAT CTTCCTTAAT CCATTTATTT GGTATATTCC TGAACGAGAA ATATATTTAT 
CAAAAGGCTT CGCATTTAAT GCAAAGGTTC ATTGAATATC TTGAGAAGTC TCTGAAGCCA 
GAACATGTTA ATACGCCTTG GTTTTCGAAA GCTCTTTACG TTTACGAAAT TATACTGGCG 
AAATCTGAGC TTCCACACCT CGAAGAGCTT TCCAAGGATG TTCTTCTGAG ATATCCATTG 
CTTTCCATGG CGAAAGTCTT TCGCATACCA GACCCTATGA AGCAGAAATT ATTTGACATA 
CTGATCAGAG TTTCTGATAT TTCCAATTTT TATTCTGCAC TGGCAACATC ACGCATACTT 
ATTTTCTACT CTAGGGATGA GTTGTATGCT AACAACATAG CTAGATCGGG AATATTATCT 
AGATTGTTGA AAGTAATTGG AAGTTTCCAA AAACTAGACA AAATAAACTT TTTAGAGTCA 
TCTTTCCTTT TATTAACCAG GAGATGTTTT GAAACGACTG AAAATGTTGA TGCTTTAATA 
CGTGCAGAAA TTAATAGAAG CTTTACAGCA AGACCGTTAG GTGGTGGGGA TGATGCTGTA 
CGTGAGTTAA CCACTATTCT AGAAGAAAAG GCGCATGTGG TCATGCGTAG TCCCTCTCAA 
TTTATTGACG TTCTTTGCGA AACAGCTCGG TTCCATGAAT TTGATGATCA AGGTGCGTTG 
GTTGATTACT CATTGAAAAG ATTTCTTGGT GAAAAAGATA AGAATACGCA AGCCTCAAGT 
ACAGAAAAAT CAGATATCTA CGAGAGAACG GGGATCATGC ATTTATTACT GTCACAACTT 
ATGGCTGCTT CTGAGAAAGA CTGGTTGTCT GAACCGGCCA ACTCCTCGGA TTTGCCTGAG 
AACAAAAAAG CCCAATTGGA CCCATCAAGA AATCCTGTAT GTGCTTACAT GATTTTTCTT 
TTGAAGCTTT TGGTCGAGTT AGTTAGCAGT TACAATCAAT GTAAATTTGA GTTCTTAACT 
TTCAGTAGAA GAAACACATA TGCAGAACGC CCCAGACCAA GAACAACTGC TATTAACTTT 
TTCCTTTATA GATTATTAGA CAAACCTGTC GGAACTGACC ACGATAAGCA TGAAGCTAAA 
AGAAGAGAGG TTATTGGAAT GCTAGCACGT TCCGTAATTA TTGGTTTCTT GGCTACTGTT 
CAAGATGATA GGACGACCAA GACTGACGTC AAGCTTGCTG ATCCTCACAT GAACTTTATT 
AGAAAATTTG CCATTGAAGC GATTATAAAA GCCATTCGCA ACGCCACTTC CTCTTCTAAA 
CTTTTGGAAA GTAATCATTT AAAGTTAGAT ATGTGGTTTA GAATCATTAC ATCGATGGTT 
TATGTCCAGG CTCCATATCT TCGCCAACTA CTTGATTCAA ATAAAGTTGA GGCAGATCAG 
TACCAATTGT GCAAACTGGT TATAGACCTC GGTCTTCCGT CAGTTATCAC GGAAGCCATG 
GCCAGTATAG ACTTAAACTA TCCTTTTTCC AAAAAGATTT TCAACGTCGC TGTTGAAGCT 
CTAAACACAA TTAGTTCGAC AAGGAACAAT TTTTCTGAGC ACTTCAAAAT TGAAGATCAT 
GATGAAGTTG AAGACGAGGT TGACGAGTCT GATAAGGAAG AGATCCCGGA TATGTTCAAA 
AACTCAGCGC TGGGAATGTA TGACGTAGAG GACATTGAAG AAGACGACGA TGACGACACA 
TCATTAATTG GAGACGATGA TGCTATGGCA TTTGTTGATA GTGATAACGG ATTTGAAGTA 
GTATTTAGTG ACGAAGATGA TGATATGGGA GAAGAAGATG CAGATGATGC TCGTTCAGAT 
TCGGAAGAAA ATGAATTATC TTCTGAAATG CAATCAAGTA CAGCGGACGG CACGGATGTG 
GACTATGAGG TTGATGATGC AGATGGACTA ATTATCAATA TTGACCAGCC CTCAGGAGAT 
GATGAAGAAA TGGCAGACTA TGATGCTAAC ATTAGCCATT CTTCTCACAG TGAAAATGAA 
GATGACGCTT CAATGGACGT CATAGAAGTT TACGACGACG AGCTAAGTTC TGGATATGAT 
GTTGATTTAA GCGATTATGA CGTAGATGAA TCTGATTGGG ACTCCGGGTT ATCCAGCCTA 
TCTATTTCTG ATGAAGATAG TGAAAGTAGC GAAGATGAGC CTATTAACTC CACCAGAATG 
GGAGATTCAA GAAGGAGATG GCTGATAGCT GAAGGCGTGG AGCTAACTGA CGACTCGCAA 
GGTGAGAGTG AAGAAGATGA TAGAGGTGTT TTCCGTGGTA TTGAACATAT ATTTTCAAAT 
GAGAATGAGC CTTTGTTTAG AGTTCATGAC GAAATGCGTC ATAGAAACCA TCATCGCTCA 
ATCAATCGTA CCCATTTTCA CTCTGCTATG AGCGCCCCCT CTTTAAGCTT ATTAAATCGT 
GGAAGAAGAA ACCAAAGCAA CCTAATAAAT CCACTAGGGC CTACAGGATT GGAACAAGTG 
GAGAACGATA TTTCAGATCA GGTGACTGTT GCTGGTTCAG GCTCTCGCCC AAGATCACAT 
CATCTTCACT TTTCGGAAGT TCTAGTCTCA GGCAGCTTTT TTGATGAACC TGTCTTAGAT 
GGCATAATTT TAAAGTCTAC GGTATCCAGA TGGAAGGATA TATTCGACAT GTTTTACGAC 
TCTAAGACTT ACGCTAACTG TATTATTCCT ACTGTTATCA ACAGATTATA CAAGGTCAGT 
TTAGCTTTGC AAAAGGACTT GGAAAACAAA CGTGAACAAG AGAAATTGAA AAATAAGAAC 
CTACTATTCA ACGAGGCTAA AGTAGAAAGC CATAACAGTT CTGATGCTAT ATCGGTAGAA 
CAAGACGATA TACAAGAAAG CAATGTTACT CATGATGACC ACGAGCCTGT ATATGTTACT 
ATTCAAGGTT CCGAGGTTGA TATAGGTGGT ACAGATATTG ATCCAGAATT CATGAATGCA 
TTGCCTGATG ATATAAGAGC CGATGTCTTT GCACAACATG TTAGAGAAAG AAGAGCAGAA 
GCTAGGCTAA ATTCTGATCA TAACGTACAC TCCAGAGAAA TCGATTCCGA TTTTCTGGAA 
GCAATTCCTG AAGACATCAG AGAAGGAATA TTGGATACTG AGGCAGAGGA ACAGAGAATG 
TTTGGTAGAA TTGGCTCTTC GGCCGATGTG ATTAGAGCAG ACGACGACGT TAGCAACAAT 
GACGAAGAAG TTGAAAACGG TTTGGATCAT GGAAATAGTA ATGATCGCAA TAACGCCGAC 
CCTGAAAAGA AAAAACCGGC TAGAATATAC TTTGCACCAT TAATTGATCG TGCCGGGATT 
GCCTCATTGA TGAAATCTGT GTTTATTTCG AAACCTTATA TTCAGCGTGA AATTTATCAT 
GAGCTTTTCT ACCGTTTATG TTCTAGCAAA CAAAATAGAA ATGATTTGAT GAATACATTT 
TTATTTATAC TAAGTGAAGG TATCATAGAT CAGCACTCTT TGGAAAAGGT TTATAACATT 
ATTTCCAGCA GGGCAATGGG GCACGCAAAA ACCACGACCG TTAGACAGTT ACCATCAGAT 
TGTACCCCGC TAACAGTGGC TAATCAAACT ATCGAAATTT TGCAAAGTCT AATTGACGCG 
GATTCTAGGC TAAAATACTT CCTGATTGCA GAACATGACA ACCTGATAGT GAACAAAGCT 
AATAACAAAT CTAGGAAAGA AGCCTTACCT GACAAAAAAT TAAGATGGCC TTTATGGCAT 
TTATTCTCAC TTTTAGATAG AAAGCTCATT ACAGACGAAA GCGTATTGAT GGATTTGTTA 
ACAAGAATAT TACAAGTTTG TACTAAAACA TTGGCTGTCC TTTCTACAAG TTCAAATGGT 
AAAGAAAATT TAAGCAAAAA GTTTCATTTA CCTAGTTTTG ATGAGGATGA TCTCATGAAA 
ATTTTATCCA TCATTATGCT TGATAGTTGT ACTACAAGAG TATTTCAACA AACACTTAAT 
ATAATTTACA ACCTCTCTAA ATTACAAGGC TGTATGTCCA TTTTCACGAA ACATTTGGTA 
TCTTTAGCTA TCAGCATAAT GAGTAAATTA AAGAGTGCTC TCGATGGGCT ATCACGTGAA 
GTAGGCACTA TCACCACCGG AATGGAGATC AATTCAGAGC TGTTACAGAA ATTTACCCTT 
CCAAGTTCTG ATCAAGCTAA ATTATTGAAA ATTTTAACTA CGGTTGATTT TCTTTACACT 
CACAAAAGAA AAGAGGAAGA GCGCAACGTT AAAGACCTTC AATCTTTATA TGATAAGATG 
AACGGCGGAC CAGTGTGGTC CTCATTGAGT GAGTGCTTAT CTCAATTTGA AAAGAGTCAA 
GCCATCAACA CTTCAGCCAC AATCTTACTT CCGTTGATCG AATCACTTAT GGTTGTATGC 
AGGCGTAGTG ACTTAAGTCA AAATAGAAAC ACTGCAGTCA AATATGAAGA TGCTAAATTA 
TTGGATTTTT CCAAAACACG TGTCGAAAAC TTATTTTTCC CATTTACAGA CGCACACAAG 
AAGTTGTTGA ATCAAATGAT TCGTTCAAAT CCAAAACTGA TGAGTGGTCC TTTCGCATTA 
TTGGTCAAGA ATCCAAAAGT TTTGGATTTT GACAACAAAA GATATTTCTT CAATGCCAAA 
TTGAAGTCTG ATAATCAAGA GCGTCCTAAA TTACCTATTA CCGTTCGCCG TGAGCAGGTT 
TTCTTGGACT CTTATCGAGC CTTATTTTTC AAGACAAACG ATGAAATCAA GAATTCTAAA 
TTAGAGATAA CATTCAAAGG AGAAAGTGGT GTCGATGCCG GTGGGGTAAC CAGAGAATGG 
TACCAAGTAC TATCGAGACA GATGTTCAAC CCTGATTATG CTCTTTTTCT TCCTGTACCA 
TCGGATAAAA CTACTTTCCA TCCTAATCGG ACATCAGGTA TCAACCCTGA GCACCTTTCT 
TTCTTTAAAT TTATCGGGAT GATTATAGGG AAAGCAATTA GAGACCAATG CTTTTTGGAT 
TGCCATTTTA GTCGTGAAGT ATACAAGAAT ATTTTGGGTA GGCCTGTATC ATTGAAGGAT 
ATGGAGTCTC TTGATCCAGA TTATTATAAA TCATTGGTGT GGATTCTTGA AAACGATATC 
ACAGATATTA TAGAGGAAAC TTTTTCTGTT GAAACTGATG ATTATGGTGA ACATAAAGTT 
ATCAATTTGA TTGAGGGGGG CAAGGATATA ATAGTTACAG AGGCCAATAA ACAGGATTAT 
GTGAAAAAGG TTGTCGAATA TAAACTTCAG ACATCTGTTA AGGAACAAAT GGATAACTTT 
TTAGTGGGTT TCTATGCCCT TATATCCAAA GATCTCATTA CAATATTTGA TGAGCAGGAA 
TTGGAGTTAT TAATCAGCGG TTTGCCTGAT ATTGATGTCG ACGACTGGAA AAACAATACT 
ACTTATGTTA ATTACACCGC AACCTGTAAG GAAGTGAGTT ATTTTTGGAG GGCAGTTAGA 
TCCTTCGACG CTGAAGAACG TGCCAAGTTG TTGCAGTTTG TTACCGGTAC TAGTAAAGTT 
CCTCTAAATG GGTTTAAGGA ACTAAGCGGT GTCAACGGTG TTTGTAAATT TTCCATCCAT 
CGTGATTTTG GTTCATCAGA AAGACTACCA TCATCACATA CCTGTTTCAA TCAACTGAAT 
TTACCTCCTT ATGAATCATA TGAGACATTG CGTGGTTCAC TATTATTGGC AATCAATGAA 
GGGCATGAAG GGTTTGGTCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010745.3
CDS	1..9807
Translation	MVLFTRCEKARKEKLAAGYKPLVDYLIDCDTPTFLERIEAIQEWDRSRDDLYVWIPILDRMDGLLLKVAEKYKYKQDPKKECEVKLVEMEAHDVDYCLKMLKFTRRLLLNTENRFVYSSGDVLMYLLNCPNFTIKLAVMRILAILGERFVIAREKIVAHNIFGDHNLRKKTLKLALSLSSSVMDEDGEHFSLVDLYFDKKKVPQKWRKLRFTHYTSNDFKKSSQQKNNINETQTSIKKVTMTTQELCEHSLQQIFDKGMALLPAESWFDFSIKASVAKAFSDDSGENIDLRNIIIETKLNAIAFVNTIFSPPQVSSKLFELDPYAFNSLTDLISLSETKIPKELRTDALFTLECISLKHVWCSDIIRNLGGNISHGLLFQILRYIAKTLREATDEIDEEYNVRFFYLISNLADVKPLHESLFAAGLIPTLLEIVSIRNCPYKRTLASATHLLETFIDNSETTTEFIENDGFTMLITSVANEIDFTLAHPETWQPPKYSVVYYSISFRELAYIRSLLKLVLKLLSTDSGDRIRNLIDSPILVSLKKILENKLVFGLTLITYTLDVVQKVINSEPTIYPVLVEAGLIPYVIDNFPKLIGPSAELLSLLPDVVSAICLNPEGLKQVKEKGLINNLFDFLLDADHARILTGGDRSTEYGTDIDELARHYPDLKANIVEALCNVIRKMPSTFRNEREFLFTSPKDQKYFFHRKNEEILTDKEEHEPAYWELLDKGTMLDTFTSVLFGMSLGNGSFSQVPQHLEARDFLAIIFMENPPYEYFTSVAISNVTEVLQYLDEKYEDYAFMDVMKVLNDQLENLNDFLNSPNDRSFFLERDGENSVRSCHSKLCRLAAILNIVTNVYIDLTTLSCKRIMQIYSYFDKRGFSLIKNLKLLFQKCALEEMYIRQHMPDSVITETMPLPIVDVSGDGPPLQIYIDDPKKGDQKGKITSVKTRNTLQMRTILYTLQSNTAILFRCFLRLSHSRNMDLEHKDLTTEVHIFENVVENVIEMLKATELEGHLPYILVLLNFNTFVFTIPKASPNSTEILQTIPAYIFYQKGGYLLYLHIIRDLFTRMTKIKDLSSLDNINYIDESNGILTLSCLINALTFYNKSMQTETMENVQSIGKYYVSIDDDYNIMKALTVPIKVMALAMILDLDKSDSLFKTQSRNVPYSVFKQLLSMLKNIFTNVNIYTKELYELHWDLIFPPIKKISLFEQVGIPGDVAANYLTDTGDDLPADNSIGLFSPEQWEKYKKLIGEDKSIYYPQPMQAQYYKGCSSKELDELRDTFFNDGLPSRIFTVLPFYPKLVNAFAKTLLQIFTKYDEPTEVFAGRILDRILETDLDDPATLSSLIHLFGIFLNEKYIYQKASHLMQRFIEYLEKSLKPEHVNTPWFSKALYVYEIILAKSELPHLEELSKDVLLRYPLLSMAKVFRIPDPMKQKLFDILIRVSDISNFYSALATSRILIFYSRDELYANNIARSGILSRLLKVIGSFQKLDKINFLESSFLLLTRRCFETTENVDALIRAEINRSFTARPLGGGDDAVRELTTILEEKAHVVMRSPSQFIDVLCETARFHEFDDQGALVDYSLKRFLGEKDKNTQASSTEKSDIYERTGIMHLLLSQLMAASEKDWLSEPANSSDLPENKKAQLDPSRNPVCAYMIFLLKLLVELVSSYNQCKFEFLTFSRRNTYAERPRPRTTAINFFLYRLLDKPVGTDHDKHEAKRREVIGMLARSVIIGFLATVQDDRTTKTDVKLADPHMNFIRKFAIEAIIKAIRNATSSSKLLESNHLKLDMWFRIITSMVYVQAPYLRQLLDSNKVEADQYQLCKLVIDLGLPSVITEAMASIDLNYPFSKKIFNVAVEALNTISSTRNNFSEHFKIEDHDEVEDEVDESDKEEIPDMFKNSALGMYDVEDIEEDDDDDTSLIGDDDAMAFVDSDNGFEVVFSDEDDDMGEEDADDARSDSEENELSSEMQSSTADGTDVDYEVDDADGLIINIDQPSGDDEEMADYDANISHSSHSENEDDASMDVIEVYDDELSSGYDVDLSDYDVDESDWDSGLSSLSISDEDSESSEDEPINSTRMGDSRRRWLIAEGVELTDDSQGESEEDDRGVFRGIEHIFSNENEPLFRVHDEMRHRNHHRSINRTHFHSAMSAPSLSLLNRGRRNQSNLINPLGPTGLEQVENDISDQVTVAGSGSRPRSHHLHFSEVLVSGSFFDEPVLDGIILKSTVSRWKDIFDMFYDSKTYANCIIPTVINRLYKVSLALQKDLENKREQEKLKNKNLLFNEAKVESHNSSDAISVEQDDIQESNVTHDDHEPVYVTIQGSEVDIGGTDIDPEFMNALPDDIRADVFAQHVRERRAEARLNSDHNVHSREIDSDFLEAIPEDIREGILDTEAEEQRMFGRIGSSADVIRADDDVSNNDEEVENGLDHGNSNDRNNADPEKKKPARIYFAPLIDRAGIASLMKSVFISKPYIQREIYHELFYRLCSSKQNRNDLMNTFLFILSEGIIDQHSLEKVYNIISSRAMGHAKTTTVRQLPSDCTPLTVANQTIEILQSLIDADSRLKYFLIAEHDNLIVNKANNKSRKEALPDKKLRWPLWHLFSLLDRKLITDESVLMDLLTRILQVCTKTLAVLSTSSNGKENLSKKFHLPSFDEDDLMKILSIIMLDSCTTRVFQQTLNIIYNLSKLQGCMSIFTKHLVSLAISIMSKLKSALDGLSREVGTITTGMEINSELLQKFTLPSSDQAKLLKILTTVDFLYTHKRKEEERNVKDLQSLYDKMNGGPVWSSLSECLSQFEKSQAINTSATILLPLIESLMVVCRRSDLSQNRNTAVKYEDAKLLDFSKTRVENLFFPFTDAHKKLLNQMIRSNPKLMSGPFALLVKNPKVLDFDNKRYFFNAKLKSDNQERPKLPITVRREQVFLDSYRALFFKTNDEIKNSKLEITFKGESGVDAGGVTREWYQVLSRQMFNPDYALFLPVPSDKTTFHPNRTSGINPEHLSFFKFIGMIIGKAIRDQCFLDCHFSREVYKNILGRPVSLKDMESLDPDYYKSLVWILENDITDIIEETFSVETDDYGEHKVINLIEGGKDIIVTEANKQDYVKKVVEYKLQTSVKEQMDNFLVGFYALISKDLITIFDEQELELLISGLPDIDVDDWKNNTTYVNYTATCKEVSYFWRAVRSFDAEERAKLLQFVTGTSKVPLNGFKELSGVNGVCKFSIHRDFGSSERLPSSHTCFNQLNLPPYESYETLRGSLLLAINEGHEGFGLA

Target ORF information:

RefSeq Version	NM_001180765.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase TOM1 (TOM1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180765.3

ORF Insert Sequence:

ATGGTGCTTT TTACTCGGTG TGAAAAGGCA AGAAAGGAGA AACTCGCCGC TGGTTATAAG 
CCCTTAGTTG ATTATTTGAT TGATTGTGAT ACTCCCACAT TTTTAGAAAG AATTGAGGCA 
ATCCAAGAGT GGGACCGATC CAGAGATGAT CTTTATGTTT GGATTCCCAT TCTAGACAGA 
ATGGACGGGC TGTTGTTAAA AGTGGCAGAG AAATATAAAT ACAAGCAAGA CCCCAAGAAG 
GAGTGTGAAG TCAAATTGGT GGAAATGGAA GCGCATGATG TTGATTATTG TTTGAAAATG 
CTAAAGTTTA CTCGTCGACT ACTTCTAAAT ACAGAAAACA GGTTTGTTTA TTCATCTGGA 
GATGTTTTAA TGTATCTACT GAATTGTCCG AATTTTACAA TAAAACTAGC GGTCATGCGA 
ATTTTAGCCA TTTTAGGGGA AAGGTTTGTG ATTGCGAGAG AAAAAATAGT TGCACATAAC 
ATATTTGGAG ATCACAACCT CAGGAAAAAA ACTCTCAAAC TAGCTTTATC TCTGTCATCT 
TCCGTCATGG ATGAAGACGG AGAGCACTTT TCTCTTGTCG ATCTATATTT TGATAAGAAA 
AAAGTGCCTC AAAAATGGAG AAAGTTGAGA TTTACACATT ATACTTCAAA CGACTTTAAA 
AAGTCAAGCC AGCAAAAAAA TAACATTAAT GAAACCCAAA CTTCCATTAA GAAGGTAACT 
ATGACGACCC AAGAACTGTG TGAACACTCA CTACAGCAAA TATTTGATAA GGGTATGGCC 
CTTTTACCCG CAGAAAGTTG GTTTGATTTC TCAATAAAAG CTTCGGTGGC GAAGGCCTTC 
AGTGATGATT CAGGTGAAAA TATAGATTTG AGAAATATAA TTATTGAAAC AAAACTGAAT 
GCTATTGCAT TTGTTAACAC CATTTTCTCG CCTCCTCAAG TAAGCTCGAA GTTATTTGAA 
TTAGATCCGT ATGCTTTTAA TAGTCTAACT GATTTGATAT CGTTGTCTGA AACCAAAATA 
CCAAAAGAGC TAAGAACGGA TGCTTTATTC ACATTGGAAT GTATATCCTT GAAGCATGTC 
TGGTGCTCAG ACATTATCAG AAATCTCGGT GGTAACATAT CGCATGGTCT GTTGTTTCAG 
ATTCTCCGTT ACATTGCCAA GACATTGAGA GAAGCGACAG ACGAAATCGA TGAAGAATAT 
AATGTGAGGT TCTTTTACTT GATATCAAAT TTAGCTGACG TTAAGCCTTT GCATGAATCT 
CTTTTCGCCG CGGGTTTAAT ACCTACTTTA TTGGAAATTG TATCTATAAG AAATTGCCCA 
TATAAGCGTA CCCTCGCATC TGCTACCCAT CTCCTAGAAA CATTTATAGA TAACAGTGAA 
ACTACCACTG AATTTATCGA AAATGATGGG TTTACTATGC TGATCACTTC AGTCGCCAAT 
GAGATTGATT TCACGCTTGC ACATCCAGAG ACGTGGCAAC CGCCAAAATA TTCAGTGGTT 
TATTATTCAA TTTCTTTTAG GGAGTTGGCA TATATTAGAA GTTTATTGAA ACTCGTTCTC 
AAATTATTAA GCACAGACTC AGGGGATAGA ATTAGAAATT TAATAGATTC GCCGATACTT 
GTTTCATTAA AGAAAATCCT AGAAAATAAA CTTGTATTCG GTTTAACATT AATAACTTAC 
ACTCTGGATG TAGTTCAGAA AGTAATAAAT AGTGAGCCTA CTATTTATCC TGTTCTGGTT 
GAAGCAGGAC TTATTCCATA TGTTATCGAT AATTTTCCAA AGTTAATTGG GCCTTCCGCC 
GAATTATTAA GTTTACTTCC AGATGTCGTT TCTGCCATAT GCTTGAATCC AGAAGGGCTG 
AAGCAAGTTA AAGAAAAGGG TCTCATAAAC AATTTGTTTG ATTTCTTACT CGATGCTGAC 
CACGCGCGTA TATTAACAGG TGGAGATCGT TCTACGGAAT ATGGTACTGA CATTGATGAA 
TTAGCAAGAC ATTATCCAGA CTTAAAGGCA AATATCGTGG AGGCTTTGTG TAACGTCATC 
AGAAAAATGC CAAGTACCTT TAGAAATGAA AGAGAGTTTC TTTTTACTTC ACCAAAAGAT 
CAAAAGTACT TTTTTCACAG AAAAAATGAG GAAATATTGA CAGATAAAGA GGAACATGAA 
CCTGCATACT GGGAGCTATT AGATAAGGGA ACTATGCTGG ACACATTTAC CAGTGTTTTG 
TTTGGTATGT CTTTAGGAAA TGGTTCTTTT TCACAGGTGC CACAACATCT GGAAGCGAGA 
GATTTTCTGG CAATAATATT TATGGAAAAT CCTCCTTATG AATACTTTAC CTCCGTGGCA 
ATCTCAAATG TTACTGAGGT CCTCCAGTAT CTGGATGAAA AATACGAAGA CTACGCATTC 
ATGGATGTTA TGAAAGTATT GAACGATCAA TTGGAGAACC TGAATGATTT TTTGAATAGT 
CCAAATGATA GAAGTTTCTT TTTAGAAAGA GATGGGGAAA ACTCGGTGCG TTCATGCCAT 
TCAAAACTTT GTCGACTTGC CGCTATTTTG AACATTGTTA CGAATGTATA CATTGACCTC 
ACTACCTTAT CTTGTAAGAG AATCATGCAG ATCTATTCTT ATTTTGACAA AAGAGGATTT 
TCTTTAATTA AAAACTTAAA GTTGCTATTC CAGAAATGTG CGTTGGAGGA AATGTACATA 
CGCCAACATA TGCCAGATTC TGTTATTACA GAAACAATGC CGTTGCCTAT AGTGGATGTT 
TCCGGAGATG GACCACCACT TCAAATTTAT ATCGATGACC CAAAAAAGGG TGATCAAAAG 
GGGAAAATTA CCAGCGTAAA AACGAGAAAT ACTCTTCAAA TGCGAACAAT ACTGTATACA 
TTACAATCAA ATACTGCAAT TCTATTTCGC TGCTTTTTAA GGTTAAGCCA CTCTAGGAAT 
ATGGATCTAG AGCATAAAGA TTTGACGACA GAAGTTCATA TATTTGAGAA CGTTGTTGAA 
AATGTTATTG AAATGTTGAA GGCCACCGAA CTCGAAGGTC ATCTACCGTA TATTCTCGTT 
TTATTAAACT TCAACACCTT TGTGTTTACT ATTCCAAAGG CTTCCCCTAA TTCCACTGAA 
ATATTACAAA CAATACCGGC TTATATTTTC TATCAAAAGG GGGGCTATTT ACTTTATTTG 
CACATTATAA GGGATCTCTT TACGAGAATG ACAAAAATAA AAGATTTATC CTCACTTGAT 
AACATAAACT ACATAGATGA GTCCAACGGT ATCCTAACCT TAAGCTGTTT AATTAATGCT 
CTCACCTTTT ACAACAAATC AATGCAAACT GAAACCATGG AGAATGTCCA AAGTATTGGT 
AAGTATTACG TTTCAATAGA TGACGATTAT AATATTATGA AAGCTTTAAC AGTTCCTATT 
AAGGTAATGG CTCTCGCAAT GATTTTAGAT TTGGATAAAT CGGATAGCCT CTTCAAGACA 
CAGTCCCGTA ATGTGCCTTA CTCAGTTTTC AAGCAACTTC TTAGTATGCT CAAAAATATA 
TTCACTAATG TGAACATCTA TACCAAGGAA TTATATGAAT TGCATTGGGA CCTAATATTC 
CCACCAATTA AAAAAATTAG CTTGTTTGAG CAAGTCGGCA TTCCCGGTGA TGTTGCGGCA 
AACTATTTAA CAGATACTGG TGATGATCTT CCTGCAGATA ACAGTATTGG CCTGTTTTCG 
CCTGAACAAT GGGAAAAATA TAAAAAATTA ATCGGTGAAG ATAAATCCAT CTACTATCCA 
CAACCAATGC AAGCTCAGTA TTACAAAGGA TGTTCCTCAA AAGAATTAGA TGAGCTGAGA 
GATACCTTTT TCAATGACGG TTTACCATCT AGGATCTTCA CTGTACTCCC TTTCTATCCT 
AAACTTGTAA ACGCGTTTGC AAAAACTTTG CTACAAATTT TTACGAAATA CGATGAACCC 
ACTGAAGTGT TTGCTGGTAG AATCTTAGAT CGAATTTTAG AGACAGATCT AGATGACCCT 
GCTACTTTAT CTTCCTTAAT CCATTTATTT GGTATATTCC TGAACGAGAA ATATATTTAT 
CAAAAGGCTT CGCATTTAAT GCAAAGGTTC ATTGAATATC TTGAGAAGTC TCTGAAGCCA 
GAACATGTTA ATACGCCTTG GTTTTCGAAA GCTCTTTACG TTTACGAAAT TATACTGGCG 
AAATCTGAGC TTCCACACCT CGAAGAGCTT TCCAAGGATG TTCTTCTGAG ATATCCATTG 
CTTTCCATGG CGAAAGTCTT TCGCATACCA GACCCTATGA AGCAGAAATT ATTTGACATA 
CTGATCAGAG TTTCTGATAT TTCCAATTTT TATTCTGCAC TGGCAACATC ACGCATACTT 
ATTTTCTACT CTAGGGATGA GTTGTATGCT AACAACATAG CTAGATCGGG AATATTATCT 
AGATTGTTGA AAGTAATTGG AAGTTTCCAA AAACTAGACA AAATAAACTT TTTAGAGTCA 
TCTTTCCTTT TATTAACCAG GAGATGTTTT GAAACGACTG AAAATGTTGA TGCTTTAATA 
CGTGCAGAAA TTAATAGAAG CTTTACAGCA AGACCGTTAG GTGGTGGGGA TGATGCTGTA 
CGTGAGTTAA CCACTATTCT AGAAGAAAAG GCGCATGTGG TCATGCGTAG TCCCTCTCAA 
TTTATTGACG TTCTTTGCGA AACAGCTCGG TTCCATGAAT TTGATGATCA AGGTGCGTTG 
GTTGATTACT CATTGAAAAG ATTTCTTGGT GAAAAAGATA AGAATACGCA AGCCTCAAGT 
ACAGAAAAAT CAGATATCTA CGAGAGAACG GGGATCATGC ATTTATTACT GTCACAACTT 
ATGGCTGCTT CTGAGAAAGA CTGGTTGTCT GAACCGGCCA ACTCCTCGGA TTTGCCTGAG 
AACAAAAAAG CCCAATTGGA CCCATCAAGA AATCCTGTAT GTGCTTACAT GATTTTTCTT 
TTGAAGCTTT TGGTCGAGTT AGTTAGCAGT TACAATCAAT GTAAATTTGA GTTCTTAACT 
TTCAGTAGAA GAAACACATA TGCAGAACGC CCCAGACCAA GAACAACTGC TATTAACTTT 
TTCCTTTATA GATTATTAGA CAAACCTGTC GGAACTGACC ACGATAAGCA TGAAGCTAAA 
AGAAGAGAGG TTATTGGAAT GCTAGCACGT TCCGTAATTA TTGGTTTCTT GGCTACTGTT 
CAAGATGATA GGACGACCAA GACTGACGTC AAGCTTGCTG ATCCTCACAT GAACTTTATT 
AGAAAATTTG CCATTGAAGC GATTATAAAA GCCATTCGCA ACGCCACTTC CTCTTCTAAA 
CTTTTGGAAA GTAATCATTT AAAGTTAGAT ATGTGGTTTA GAATCATTAC ATCGATGGTT 
TATGTCCAGG CTCCATATCT TCGCCAACTA CTTGATTCAA ATAAAGTTGA GGCAGATCAG 
TACCAATTGT GCAAACTGGT TATAGACCTC GGTCTTCCGT CAGTTATCAC GGAAGCCATG 
GCCAGTATAG ACTTAAACTA TCCTTTTTCC AAAAAGATTT TCAACGTCGC TGTTGAAGCT 
CTAAACACAA TTAGTTCGAC AAGGAACAAT TTTTCTGAGC ACTTCAAAAT TGAAGATCAT 
GATGAAGTTG AAGACGAGGT TGACGAGTCT GATAAGGAAG AGATCCCGGA TATGTTCAAA 
AACTCAGCGC TGGGAATGTA TGACGTAGAG GACATTGAAG AAGACGACGA TGACGACACA 
TCATTAATTG GAGACGATGA TGCTATGGCA TTTGTTGATA GTGATAACGG ATTTGAAGTA 
GTATTTAGTG ACGAAGATGA TGATATGGGA GAAGAAGATG CAGATGATGC TCGTTCAGAT 
TCGGAAGAAA ATGAATTATC TTCTGAAATG CAATCAAGTA CAGCGGACGG CACGGATGTG 
GACTATGAGG TTGATGATGC AGATGGACTA ATTATCAATA TTGACCAGCC CTCAGGAGAT 
GATGAAGAAA TGGCAGACTA TGATGCTAAC ATTAGCCATT CTTCTCACAG TGAAAATGAA 
GATGACGCTT CAATGGACGT CATAGAAGTT TACGACGACG AGCTAAGTTC TGGATATGAT 
GTTGATTTAA GCGATTATGA CGTAGATGAA TCTGATTGGG ACTCCGGGTT ATCCAGCCTA 
TCTATTTCTG ATGAAGATAG TGAAAGTAGC GAAGATGAGC CTATTAACTC CACCAGAATG 
GGAGATTCAA GAAGGAGATG GCTGATAGCT GAAGGCGTGG AGCTAACTGA CGACTCGCAA 
GGTGAGAGTG AAGAAGATGA TAGAGGTGTT TTCCGTGGTA TTGAACATAT ATTTTCAAAT 
GAGAATGAGC CTTTGTTTAG AGTTCATGAC GAAATGCGTC ATAGAAACCA TCATCGCTCA 
ATCAATCGTA CCCATTTTCA CTCTGCTATG AGCGCCCCCT CTTTAAGCTT ATTAAATCGT 
GGAAGAAGAA ACCAAAGCAA CCTAATAAAT CCACTAGGGC CTACAGGATT GGAACAAGTG 
GAGAACGATA TTTCAGATCA GGTGACTGTT GCTGGTTCAG GCTCTCGCCC AAGATCACAT 
CATCTTCACT TTTCGGAAGT TCTAGTCTCA GGCAGCTTTT TTGATGAACC TGTCTTAGAT 
GGCATAATTT TAAAGTCTAC GGTATCCAGA TGGAAGGATA TATTCGACAT GTTTTACGAC 
TCTAAGACTT ACGCTAACTG TATTATTCCT ACTGTTATCA ACAGATTATA CAAGGTCAGT 
TTAGCTTTGC AAAAGGACTT GGAAAACAAA CGTGAACAAG AGAAATTGAA AAATAAGAAC 
CTACTATTCA ACGAGGCTAA AGTAGAAAGC CATAACAGTT CTGATGCTAT ATCGGTAGAA 
CAAGACGATA TACAAGAAAG CAATGTTACT CATGATGACC ACGAGCCTGT ATATGTTACT 
ATTCAAGGTT CCGAGGTTGA TATAGGTGGT ACAGATATTG ATCCAGAATT CATGAATGCA 
TTGCCTGATG ATATAAGAGC CGATGTCTTT GCACAACATG TTAGAGAAAG AAGAGCAGAA 
GCTAGGCTAA ATTCTGATCA TAACGTACAC TCCAGAGAAA TCGATTCCGA TTTTCTGGAA 
GCAATTCCTG AAGACATCAG AGAAGGAATA TTGGATACTG AGGCAGAGGA ACAGAGAATG 
TTTGGTAGAA TTGGCTCTTC GGCCGATGTG ATTAGAGCAG ACGACGACGT TAGCAACAAT 
GACGAAGAAG TTGAAAACGG TTTGGATCAT GGAAATAGTA ATGATCGCAA TAACGCCGAC 
CCTGAAAAGA AAAAACCGGC TAGAATATAC TTTGCACCAT TAATTGATCG TGCCGGGATT 
GCCTCATTGA TGAAATCTGT GTTTATTTCG AAACCTTATA TTCAGCGTGA AATTTATCAT 
GAGCTTTTCT ACCGTTTATG TTCTAGCAAA CAAAATAGAA ATGATTTGAT GAATACATTT 
TTATTTATAC TAAGTGAAGG TATCATAGAT CAGCACTCTT TGGAAAAGGT TTATAACATT 
ATTTCCAGCA GGGCAATGGG GCACGCAAAA ACCACGACCG TTAGACAGTT ACCATCAGAT 
TGTACCCCGC TAACAGTGGC TAATCAAACT ATCGAAATTT TGCAAAGTCT AATTGACGCG 
GATTCTAGGC TAAAATACTT CCTGATTGCA GAACATGACA ACCTGATAGT GAACAAAGCT 
AATAACAAAT CTAGGAAAGA AGCCTTACCT GACAAAAAAT TAAGATGGCC TTTATGGCAT 
TTATTCTCAC TTTTAGATAG AAAGCTCATT ACAGACGAAA GCGTATTGAT GGATTTGTTA 
ACAAGAATAT TACAAGTTTG TACTAAAACA TTGGCTGTCC TTTCTACAAG TTCAAATGGT 
AAAGAAAATT TAAGCAAAAA GTTTCATTTA CCTAGTTTTG ATGAGGATGA TCTCATGAAA 
ATTTTATCCA TCATTATGCT TGATAGTTGT ACTACAAGAG TATTTCAACA AACACTTAAT 
ATAATTTACA ACCTCTCTAA ATTACAAGGC TGTATGTCCA TTTTCACGAA ACATTTGGTA 
TCTTTAGCTA TCAGCATAAT GAGTAAATTA AAGAGTGCTC TCGATGGGCT ATCACGTGAA 
GTAGGCACTA TCACCACCGG AATGGAGATC AATTCAGAGC TGTTACAGAA ATTTACCCTT 
CCAAGTTCTG ATCAAGCTAA ATTATTGAAA ATTTTAACTA CGGTTGATTT TCTTTACACT 
CACAAAAGAA AAGAGGAAGA GCGCAACGTT AAAGACCTTC AATCTTTATA TGATAAGATG 
AACGGCGGAC CAGTGTGGTC CTCATTGAGT GAGTGCTTAT CTCAATTTGA AAAGAGTCAA 
GCCATCAACA CTTCAGCCAC AATCTTACTT CCGTTGATCG AATCACTTAT GGTTGTATGC 
AGGCGTAGTG ACTTAAGTCA AAATAGAAAC ACTGCAGTCA AATATGAAGA TGCTAAATTA 
TTGGATTTTT CCAAAACACG TGTCGAAAAC TTATTTTTCC CATTTACAGA CGCACACAAG 
AAGTTGTTGA ATCAAATGAT TCGTTCAAAT CCAAAACTGA TGAGTGGTCC TTTCGCATTA 
TTGGTCAAGA ATCCAAAAGT TTTGGATTTT GACAACAAAA GATATTTCTT CAATGCCAAA 
TTGAAGTCTG ATAATCAAGA GCGTCCTAAA TTACCTATTA CCGTTCGCCG TGAGCAGGTT 
TTCTTGGACT CTTATCGAGC CTTATTTTTC AAGACAAACG ATGAAATCAA GAATTCTAAA 
TTAGAGATAA CATTCAAAGG AGAAAGTGGT GTCGATGCCG GTGGGGTAAC CAGAGAATGG 
TACCAAGTAC TATCGAGACA GATGTTCAAC CCTGATTATG CTCTTTTTCT TCCTGTACCA 
TCGGATAAAA CTACTTTCCA TCCTAATCGG ACATCAGGTA TCAACCCTGA GCACCTTTCT 
TTCTTTAAAT TTATCGGGAT GATTATAGGG AAAGCAATTA GAGACCAATG CTTTTTGGAT 
TGCCATTTTA GTCGTGAAGT ATACAAGAAT ATTTTGGGTA GGCCTGTATC ATTGAAGGAT 
ATGGAGTCTC TTGATCCAGA TTATTATAAA TCATTGGTGT GGATTCTTGA AAACGATATC 
ACAGATATTA TAGAGGAAAC TTTTTCTGTT GAAACTGATG ATTATGGTGA ACATAAAGTT 
ATCAATTTGA TTGAGGGGGG CAAGGATATA ATAGTTACAG AGGCCAATAA ACAGGATTAT 
GTGAAAAAGG TTGTCGAATA TAAACTTCAG ACATCTGTTA AGGAACAAAT GGATAACTTT 
TTAGTGGGTT TCTATGCCCT TATATCCAAA GATCTCATTA CAATATTTGA TGAGCAGGAA 
TTGGAGTTAT TAATCAGCGG TTTGCCTGAT ATTGATGTCG ACGACTGGAA AAACAATACT 
ACTTATGTTA ATTACACCGC AACCTGTAAG GAAGTGAGTT ATTTTTGGAG GGCAGTTAGA 
TCCTTCGACG CTGAAGAACG TGCCAAGTTG TTGCAGTTTG TTACCGGTAC TAGTAAAGTT 
CCTCTAAATG GGTTTAAGGA ACTAAGCGGT GTCAACGGTG TTTGTAAATT TTCCATCCAT 
CGTGATTTTG GTTCATCAGA AAGACTACCA TCATCACATA CCTGTTTCAA TCAACTGAAT 
TTACCTCCTT ATGAATCATA TGAGACATTG CGTGGTTCAC TATTATTGGC AATCAATGAA 
GGGCATGAAG GGTTTGGTCT TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TOM1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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