THI3 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following THI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi01025 NM_001180139.1
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Saccharomyces cerevisiae S288C branched-chain-2-oxoacid decarboxylase THI3 (THI3), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi01025
    Clone ID Related Accession (Same CDS sequence) NM_001180139.1
    Accession Version NM_001180139.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product branched-chain-2-oxoacid decarboxylase THI3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTA GCTATACACA GAGATATGCA CTGCCGAAGT GTATAGCAAT ATCAGATTAT 
    CTTTTCCATC GGCTCAACCA GCTGAACATA CATACCATAT TTGGACTCTC CGGAGAATTT
    AGCATGCCGT TGCTGGATAA ACTATACAAC ATTCCGAACT TACGATGGGC CGGTAATTCT
    AATGAGTTAA ATGCTGCCTA CGCAGCAGAT GGATACTCAC GACTAAAAGG CTTGGGATGT
    CTCATAACAA CCTTTGGTGT AGGCGAATTA TCGGCAATCA ATGGCGTGGC CGGATCTTAC
    GCTGAACATG TAGGAATACT TCACATAGTG GGTATGCCGC CAACAAGTGC ACAAACGAAA
    CAACTACTAC TGCATCATAC TCTGGGCAAT GGTGATTTCA CGGTATTTCA TAGAATAGCC
    AGTGATGTAG CATGCTATAC AACATTGATT ATTGACTCTG AATTATGTGC CGACGAAGTC
    GATAAGTGCA TCAAAAAGGC TTGGATAGAA CAGAGGCCAG TATACATGGG CATGCCTGTC
    AACCAGGTAA ATCTCCCGAT TGAATCAGCA AGGCTTAATA CACCTCTGGA TTTACAATTG
    CATAAAAACG ACCCAGACGT AGAGAAAGAA GTTATTTCTC GAATATTGAG TTTTATATAC
    AAAAGCCAGA ATCCGGCAAT CATCGTAGAT GCATGTACTA GTCGACAGAA TTTAATCGAG
    GAGACTAAAG AGCTTTGTAA TAGGCTTAAA TTTCCAGTTT TTGTTACACC TATGGGTAAG
    GGTACAGTAA ACGAAACAGA CCCGCAATTT GGGGGCGTAT TCACGGGCTC GATATCAGCC
    CCAGAAGTAA GAGAAGTAGT TGATTTTGCC GATTTTATCA TCGTCATTGG TTGCATGCTC
    TCCGAATTCA GCACGTCAAC TTTCCACTTC CAATATAAAA CTAAGAATTG TGCGCTACTA
    TATTCTACAT CTGTGAAATT GAAAAATGCC ACATATCCTG ACTTGAGCAT TAAATTACTA
    CTACAGAAAA TATTAGCAAA TCTTGATGAA TCTAAACTGT CTTACCAACC AAGCGAACAA
    CCCAGTATGA TGGTTCCAAG ACCTTACCCA GCAGGAAATG TCCTCTTGAG ACAAGAATGG
    GTCTGGAATG AAATATCCCA TTGGTTCCAA CCAGGTGACA TAATCATAAC AGAAACTGGT
    GCTTCTGCAT TTGGAGTTAA CCAGACCAGA TTTCCGGTAA ATACACTAGG TATTTCGCAA
    GCTCTTTGGG GATCTGTCGG ATATACAATG GGGGCGTGTC TTGGGGCAGA ATTTGCTGTT
    CAAGAGATAA ACAAGGATAA ATTCCCCGCA ACTAAACATA GAGTTATTCT GTTTATGGGT
    GACGGTGCTT TCCAATTGAC AGTTCAAGAA TTATCCACAA TTGTTAAGTG GGGATTGACA
    CCTTATATTT TTGTGATGAA TAACCAAGGT TACTCTGTGG ACAGGTTTTT GCATCACAGG
    TCAGATGCTA GTTATTACGA TATCCAACCT TGGAACTACT TGGGATTATT GCGAGTATTT
    GGTTGCACGA ACTACGAAAC GAAAAAAATT ATTACTGTTG GAGAATTCAG ATCCATGATC
    AGTGACCCAA ACTTTGCGAC CAATGACAAA ATTCGGATGA TAGAGATTAT GCTACCACCA
    AGGGATGTTC CACAGGCTCT GCTTGACAGG TGGGTGGTAG AAAAAGAACA GAGCAAACAA
    GTGCAAGAGG AGAACGAAAA TTCTAGCGCA GTAAATACGC CAACTCCAGA ATTCCAACCA
    CTTCTAAAAA AAAATCAAGT TGGATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_010203.1
    CDS1..1830
    Translation

    Target ORF information:

    RefSeq Version NM_001180139.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C branched-chain-2-oxoacid decarboxylase THI3 (THI3), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001180139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTA GCTATACACA GAGATATGCA CTGCCGAAGT GTATAGCAAT ATCAGATTAT 
    CTTTTCCATC GGCTCAACCA GCTGAACATA CATACCATAT TTGGACTCTC CGGAGAATTT
    AGCATGCCGT TGCTGGATAA ACTATACAAC ATTCCGAACT TACGATGGGC CGGTAATTCT
    AATGAGTTAA ATGCTGCCTA CGCAGCAGAT GGATACTCAC GACTAAAAGG CTTGGGATGT
    CTCATAACAA CCTTTGGTGT AGGCGAATTA TCGGCAATCA ATGGCGTGGC CGGATCTTAC
    GCTGAACATG TAGGAATACT TCACATAGTG GGTATGCCGC CAACAAGTGC ACAAACGAAA
    CAACTACTAC TGCATCATAC TCTGGGCAAT GGTGATTTCA CGGTATTTCA TAGAATAGCC
    AGTGATGTAG CATGCTATAC AACATTGATT ATTGACTCTG AATTATGTGC CGACGAAGTC
    GATAAGTGCA TCAAAAAGGC TTGGATAGAA CAGAGGCCAG TATACATGGG CATGCCTGTC
    AACCAGGTAA ATCTCCCGAT TGAATCAGCA AGGCTTAATA CACCTCTGGA TTTACAATTG
    CATAAAAACG ACCCAGACGT AGAGAAAGAA GTTATTTCTC GAATATTGAG TTTTATATAC
    AAAAGCCAGA ATCCGGCAAT CATCGTAGAT GCATGTACTA GTCGACAGAA TTTAATCGAG
    GAGACTAAAG AGCTTTGTAA TAGGCTTAAA TTTCCAGTTT TTGTTACACC TATGGGTAAG
    GGTACAGTAA ACGAAACAGA CCCGCAATTT GGGGGCGTAT TCACGGGCTC GATATCAGCC
    CCAGAAGTAA GAGAAGTAGT TGATTTTGCC GATTTTATCA TCGTCATTGG TTGCATGCTC
    TCCGAATTCA GCACGTCAAC TTTCCACTTC CAATATAAAA CTAAGAATTG TGCGCTACTA
    TATTCTACAT CTGTGAAATT GAAAAATGCC ACATATCCTG ACTTGAGCAT TAAATTACTA
    CTACAGAAAA TATTAGCAAA TCTTGATGAA TCTAAACTGT CTTACCAACC AAGCGAACAA
    CCCAGTATGA TGGTTCCAAG ACCTTACCCA GCAGGAAATG TCCTCTTGAG ACAAGAATGG
    GTCTGGAATG AAATATCCCA TTGGTTCCAA CCAGGTGACA TAATCATAAC AGAAACTGGT
    GCTTCTGCAT TTGGAGTTAA CCAGACCAGA TTTCCGGTAA ATACACTAGG TATTTCGCAA
    GCTCTTTGGG GATCTGTCGG ATATACAATG GGGGCGTGTC TTGGGGCAGA ATTTGCTGTT
    CAAGAGATAA ACAAGGATAA ATTCCCCGCA ACTAAACATA GAGTTATTCT GTTTATGGGT
    GACGGTGCTT TCCAATTGAC AGTTCAAGAA TTATCCACAA TTGTTAAGTG GGGATTGACA
    CCTTATATTT TTGTGATGAA TAACCAAGGT TACTCTGTGG ACAGGTTTTT GCATCACAGG
    TCAGATGCTA GTTATTACGA TATCCAACCT TGGAACTACT TGGGATTATT GCGAGTATTT
    GGTTGCACGA ACTACGAAAC GAAAAAAATT ATTACTGTTG GAGAATTCAG ATCCATGATC
    AGTGACCCAA ACTTTGCGAC CAATGACAAA ATTCGGATGA TAGAGATTAT GCTACCACCA
    AGGGATGTTC CACAGGCTCT GCTTGACAGG TGGGTGGTAG AAAAAGAACA GAGCAAACAA
    GTGCAAGAGG AGAACGAAAA TTCTAGCGCA GTAAATACGC CAACTCCAGA ATTCCAACCA
    CTTCTAAAAA AAAATCAAGT TGGATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
    Nature387(6632 Suppl)75-8(1997 May)
    Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG