ORF » Species Summary » Schizosaccharomyces pombe » SPAC12G12.01c cDNA ORF clone

SPAC12G12.01c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPAC12G12.01c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC12G12.01c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSj04875 XM_001712987.2
Latest version!		 Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OSc101738 NM_001355976.1
Latest version!		 Schizosaccharomyces pombe ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPAC12G12.01c ( XM_001712987.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> XP_001713039.1 Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA.
    Price & Availability↑
    CloneID OSj04875
    Clone ID Related Accession (Same CDS sequence) XM_001712987.2
    Accession Version XM_001712987.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-30
    Organism Schizosaccharomyces pombe 972h-
    Product ubiquitin-protein ligase E3
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001712987.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTTAAATA GAAGTAAAAA GAGGAATGGC TGTTATTTTT GGGTTAACGG CTCCTCAGAT 
    GAGATTCGAT ATGTCGCCGT CAAAGCTTCC CCTAAAGTAA ATTGGAAAAC TCACATTATT 
    TGGAGATCAT TGAAAAATGT TAAATGTATA GACTCTTTTC ACGGAAACAA TGAAATATTG 
    GGTGCTGGTT CTTCTACCGG AAATATATCA TTGCTTTCTG TTAAGCATCC AGAATTTCAA 
    GCAGTAGTAA CACCTGGATA TGCTCGCCCT TGCAATAGTT TGGCATTTTC TGAAACTGAA 
    CATCTTGTTG CTGGATTTGC AAAATCTCGA AACGAGTCTT CCTTGAAGTT ATGGGACTTG 
    AATTCATTAC TGTCTGACCC GAAGAGTAGT CCCTTAATGC AGTCTTCTAC TCTAGACGGA 
    GTTTCTTCCG TTTGTTATAA GAAAGACACT CCTCTTTTGC TTACCGGATC AACTTCGCGT 
    TCTGTCCATA TAATTGATAC TAGGCAGCAA CTAGACTCAG TTTCTTCCGT CAATACGCAA 
    TATTATAGTA ATATAGTTGT TGATCCATTT TCTCCTAACT ATTTTGCCGC AAATTCTTAT 
    GATGGTGACA TTGCGATTTT TGATACTCGA TACTTCAAAT CCGATAATTA TTTACAAATT 
    ATTTTACGGA ACGAAAATAA AAAGCCCAAA AATCCACAAT TATTTGCACT GAAATATTCT 
    GAATGGAAAC CTGGACAATT GGCTGTTTTG TCAAACAATT CGATTACACT TCGTCAATTA 
    TTGCCTTGTG TCAATGGAAA TGAAGGATCC GCAAATAATT CTGTCTTTGT AAATTATGAG 
    AAGAAATATC CTGTTAAACC AAATTCACAA TGTTCTGGCA TTGATTTTTT TACTCCTTCT 
    ACAGCCTTTC CAACTCATGT TCAAATTTTG GGTGTCATAA ACGAACAACC GAAACTTTTT 
    TCTGTCCATG ATGAGGTCAT TCCCTTTTCA TTTAACCCTT ATAATGATTT GATATTTTCC 
    TTCAAAGAAA AACTGTATCC TTTAAACTCT TCACCATTTA ACACACTTTC TGATGTCCCA 
    CAATTTGATG TCAGCGAATT TGTCGATGAA AACTCTTTCG ATTCCTCGTC TTCTTGTTCC 
    TCCAAAGTCT TTCTAACGAC AAGAAACAAC TCAATCAATT CAGAGGATTC CGCTCATGAA 
    GTATTGCTTT CCTATAACCG CGTCCTTGGA TCTGATATTC AAGGGACAAT ACTAGATCGC 
    GTCAAAAAGG GCTACCAATT TGATTCCCAA AAAAACTCAG AATTAGTTTC TGATTTATAC 
    TTAAAAGATC TATGGTCTTG GATTCACTTA AGCCATAGAC AATCAGAAGA ATCACTGTTT 
    GGAGACACAG GAGATACAGA TTTCAGCTAT CAAGGTGCAC TAGGTATTTG GTTTATGGAT 
    ACTGAATTGA CTAGCATGTC CGATGTTTTT GAAGCAAAAG AAAGCAAATT TTTAGAAAAA 
    AAAATTTTAA GACTTGCTAG AGATGTTATT GAGAGACTTG ATTTAGATAT ATTTACGTCT 
    ATCCAAACTA AAAGGCCTTT ACGCCAACTC GCTTTACTTG CCTGTGGATT AGGAATGTCA 
    AATGATGACT TGTTGTTGGA AATTAGAAGA CTGATTCGCA AGAATGAGCA TGTAAAAGCC 
    GCTGGGTTAG CACTTTTCCA CGGAAAAATT GAAAACGTAG TCCGTATATT ATCAAGTGGG 
    AATGAGCTGG AGAAGACCAT ATCCACAGCA GTTGCCGGAT ACATTACATC GCAAGGCTTA 
    AGTAACTTTG GAAGTGACTC ATTGTGGAAA GAAATGTCTC GTAACTTAAG TACAGAATTG 
    GAAGATCCAT ACTTACGTGC AATATTTGCG TATGTTTCCA ACTCCGATTG GAGAGATGTA 
    TTAGATGAAG TTTCTCTCTC ATTAAAAGAT CGTCTTGGAA TTGCTCTTCG CTTTTTACCA 
    GATGACGATT TGAGTAATTA TTTGTGCGAT TTATGTCACA CTACAGTGCA ATCAGGCGAC 
    CCAGAAGGCC TGTTACTTAC AGGTTTAACT CCTTTGGGAA TGGAACTTCT GCAGAACTAC 
    ATTGATCATA CAAGCGATGT TCAAACTGCT GCATTGATTG CTGCATTTGT GGTACCTAAG 
    AAATTTCTGG ATAAACGTGC AGAGGATTGG ACTGAAAGTT ATAGAGAGCT TCTAAACCGC 
    TGGAAACTTT ACCGTGAGCG TGCAAAGTTT GATATCTTTC GGACAGAACT CTCAAAAAAT 
    CACACAGGAG AAATAACCAG AAAGGCTACA GAGCCATCTA TACGCATTAT CTGCAATTTT 
    TGTAGAAAAC CAATTTTTCC TTTCTCCAAT AGGAATGAAT GTAATAATTT ACCAACGCCA 
    ATTCAACGAG GTGTCAGTAA AGCAGGCCCA GCAAAACACT TGGGAAAAAG TTGTCCTCAT 
    TGCGGACAGC CTCTTCCAAA GTGCTCTGTA TGTGGGTTTT CTTTAGGAGA TGAAGATGTT 
    CCTCAGAAAG ATGACTTTTC TCAAAAACCT CAAAACTATG TCAAAGAAGT TAATCTTCAG 
    AAATCTAGAT TTGGGTTATG GTTTAGCTTT TGTCTAAATT GTGGCCATGG AGCTCACGCA 
    TCTCATGCTT CAGAATGGTT TTCAACTCAT ACAATATGTC CGGTTCCTAA CTGTGATTGT 
    GAATGCAAAC TGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001713039.1
    CDS401..3118
    Translation

    Target ORF information:

    RefSeq Version XM_001712987.2
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001712987.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTTAAATA GAAGTAAAAA GAGGAATGGC TGTTATTTTT GGGTTAACGG CTCCTCAGAT 
    GAGATTCGAT ATGTCGCCGT CAAAGCTTCC CCTAAAGTAA ATTGGAAAAC TCACATTATT 
    TGGAGATCAT TGAAAAATGT TAAATGTATA GACTCTTTTC ACGGAAACAA TGAAATATTG 
    GGTGCTGGTT CTTCTACCGG AAATATATCA TTGCTTTCTG TTAAGCATCC AGAATTTCAA 
    GCAGTAGTAA CACCTGGATA TGCTCGCCCT TGCAATAGTT TGGCATTTTC TGAAACTGAA 
    CATCTTGTTG CTGGATTTGC AAAATCTCGA AACGAGTCTT CCTTGAAGTT ATGGGACTTG 
    AATTCATTAC TGTCTGACCC GAAGAGTAGT CCCTTAATGC AGTCTTCTAC TCTAGACGGA 
    GTTTCTTCCG TTTGTTATAA GAAAGACACT CCTCTTTTGC TTACCGGATC AACTTCGCGT 
    TCTGTCCATA TAATTGATAC TAGGCAGCAA CTAGACTCAG TTTCTTCCGT CAATACGCAA 
    TATTATAGTA ATATAGTTGT TGATCCATTT TCTCCTAACT ATTTTGCCGC AAATTCTTAT 
    GATGGTGACA TTGCGATTTT TGATACTCGA TACTTCAAAT CCGATAATTA TTTACAAATT 
    ATTTTACGGA ACGAAAATAA AAAGCCCAAA AATCCACAAT TATTTGCACT GAAATATTCT 
    GAATGGAAAC CTGGACAATT GGCTGTTTTG TCAAACAATT CGATTACACT TCGTCAATTA 
    TTGCCTTGTG TCAATGGAAA TGAAGGATCC GCAAATAATT CTGTCTTTGT AAATTATGAG 
    AAGAAATATC CTGTTAAACC AAATTCACAA TGTTCTGGCA TTGATTTTTT TACTCCTTCT 
    ACAGCCTTTC CAACTCATGT TCAAATTTTG GGTGTCATAA ACGAACAACC GAAACTTTTT 
    TCTGTCCATG ATGAGGTCAT TCCCTTTTCA TTTAACCCTT ATAATGATTT GATATTTTCC 
    TTCAAAGAAA AACTGTATCC TTTAAACTCT TCACCATTTA ACACACTTTC TGATGTCCCA 
    CAATTTGATG TCAGCGAATT TGTCGATGAA AACTCTTTCG ATTCCTCGTC TTCTTGTTCC 
    TCCAAAGTCT TTCTAACGAC AAGAAACAAC TCAATCAATT CAGAGGATTC CGCTCATGAA 
    GTATTGCTTT CCTATAACCG CGTCCTTGGA TCTGATATTC AAGGGACAAT ACTAGATCGC 
    GTCAAAAAGG GCTACCAATT TGATTCCCAA AAAAACTCAG AATTAGTTTC TGATTTATAC 
    TTAAAAGATC TATGGTCTTG GATTCACTTA AGCCATAGAC AATCAGAAGA ATCACTGTTT 
    GGAGACACAG GAGATACAGA TTTCAGCTAT CAAGGTGCAC TAGGTATTTG GTTTATGGAT 
    ACTGAATTGA CTAGCATGTC CGATGTTTTT GAAGCAAAAG AAAGCAAATT TTTAGAAAAA 
    AAAATTTTAA GACTTGCTAG AGATGTTATT GAGAGACTTG ATTTAGATAT ATTTACGTCT 
    ATCCAAACTA AAAGGCCTTT ACGCCAACTC GCTTTACTTG CCTGTGGATT AGGAATGTCA 
    AATGATGACT TGTTGTTGGA AATTAGAAGA CTGATTCGCA AGAATGAGCA TGTAAAAGCC 
    GCTGGGTTAG CACTTTTCCA CGGAAAAATT GAAAACGTAG TCCGTATATT ATCAAGTGGG 
    AATGAGCTGG AGAAGACCAT ATCCACAGCA GTTGCCGGAT ACATTACATC GCAAGGCTTA 
    AGTAACTTTG GAAGTGACTC ATTGTGGAAA GAAATGTCTC GTAACTTAAG TACAGAATTG 
    GAAGATCCAT ACTTACGTGC AATATTTGCG TATGTTTCCA ACTCCGATTG GAGAGATGTA 
    TTAGATGAAG TTTCTCTCTC ATTAAAAGAT CGTCTTGGAA TTGCTCTTCG CTTTTTACCA 
    GATGACGATT TGAGTAATTA TTTGTGCGAT TTATGTCACA CTACAGTGCA ATCAGGCGAC 
    CCAGAAGGCC TGTTACTTAC AGGTTTAACT CCTTTGGGAA TGGAACTTCT GCAGAACTAC 
    ATTGATCATA CAAGCGATGT TCAAACTGCT GCATTGATTG CTGCATTTGT GGTACCTAAG 
    AAATTTCTGG ATAAACGTGC AGAGGATTGG ACTGAAAGTT ATAGAGAGCT TCTAAACCGC 
    TGGAAACTTT ACCGTGAGCG TGCAAAGTTT GATATCTTTC GGACAGAACT CTCAAAAAAT 
    CACACAGGAG AAATAACCAG AAAGGCTACA GAGCCATCTA TACGCATTAT CTGCAATTTT 
    TGTAGAAAAC CAATTTTTCC TTTCTCCAAT AGGAATGAAT GTAATAATTT ACCAACGCCA 
    ATTCAACGAG GTGTCAGTAA AGCAGGCCCA GCAAAACACT TGGGAAAAAG TTGTCCTCAT 
    TGCGGACAGC CTCTTCCAAA GTGCTCTGTA TGTGGGTTTT CTTTAGGAGA TGAAGATGTT 
    CCTCAGAAAG ATGACTTTTC TCAAAAACCT CAAAACTATG TCAAAGAAGT TAATCTTCAG 
    AAATCTAGAT TTGGGTTATG GTTTAGCTTT TGTCTAAATT GTGGCCATGG AGCTCACGCA 
    TCTCATGCTT CAGAATGGTT TTCAACTCAT ACAATATGTC CGGTTCCTAA CTGTGATTGT 
    GAATGCAAAC TGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    SPAC12G12.01c ( NM_001355976.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342800.1 Schizosaccharomyces pombe ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA.
    Price & Availability↑
    CloneID OSc101738
    Clone ID Related Accession (Same CDS sequence) NM_001355976.1
    Accession Version NM_001355976.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product ubiquitin-protein ligase E3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTTAAATA GAAGTAAAAA GAGGAATGGC TGTTATTTTT GGGTTAACGG CTCCTCAGAT 
    GAGATTCGAT ATGTCGCCGT CAAAGCTTCC CCTAAAGTAA ATTGGAAAAC TCACATTATT 
    TGGAGATCAT TGAAAAATGT TAAATGTATA GACTCTTTTC ACGGAAACAA TGAAATATTG 
    GGTGCTGGTT CTTCTACCGG AAATATATCA TTGCTTTCTG TTAAGCATCC AGAATTTCAA 
    GCAGTAGTAA CACCTGGATA TGCTCGCCCT TGCAATAGTT TGGCATTTTC TGAAACTGAA 
    CATCTTGTTG CTGGATTTGC AAAATCTCGA AACGAGTCTT CCTTGAAGTT ATGGGACTTG 
    AATTCATTAC TGTCTGACCC GAAGAGTAGT CCCTTAATGC AGTCTTCTAC TCTAGACGGA 
    GTTTCTTCCG TTTGTTATAA GAAAGACACT CCTCTTTTGC TTACCGGATC AACTTCGCGT 
    TCTGTCCATA TAATTGATAC TAGGCAGCAA CTAGACTCAG TTTCTTCCGT CAATACGCAA 
    TATTATAGTA ATATAGTTGT TGATCCATTT TCTCCTAACT ATTTTGCCGC AAATTCTTAT 
    GATGGTGACA TTGCGATTTT TGATACTCGA TACTTCAAAT CCGATAATTA TTTACAAATT 
    ATTTTACGGA ACGAAAATAA AAAGCCCAAA AATCCACAAT TATTTGCACT GAAATATTCT 
    GAATGGAAAC CTGGACAATT GGCTGTTTTG TCAAACAATT CGATTACACT TCGTCAATTA 
    TTGCCTTGTG TCAATGGAAA TGAAGGATCC GCAAATAATT CTGTCTTTGT AAATTATGAG 
    AAGAAATATC CTGTTAAACC AAATTCACAA TGTTCTGGCA TTGATTTTTT TACTCCTTCT 
    ACAGCCTTTC CAACTCATGT TCAAATTTTG GGTGTCATAA ACGAACAACC GAAACTTTTT 
    TCTGTCCATG ATGAGGTCAT TCCCTTTTCA TTTAACCCTT ATAATGATTT GATATTTTCC 
    TTCAAAGAAA AACTGTATCC TTTAAACTCT TCACCATTTA ACACACTTTC TGATGTCCCA 
    CAATTTGATG TCAGCGAATT TGTCGATGAA AACTCTTTCG ATTCCTCGTC TTCTTGTTCC 
    TCCAAAGTCT TTCTAACGAC AAGAAACAAC TCAATCAATT CAGAGGATTC CGCTCATGAA 
    GTATTGCTTT CCTATAACCG CGTCCTTGGA TCTGATATTC AAGGGACAAT ACTAGATCGC 
    GTCAAAAAGG GCTACCAATT TGATTCCCAA AAAAACTCAG AATTAGTTTC TGATTTATAC 
    TTAAAAGATC TATGGTCTTG GATTCACTTA AGCCATAGAC AATCAGAAGA ATCACTGTTT 
    GGAGACACAG GAGATACAGA TTTCAGCTAT CAAGGTGCAC TAGGTATTTG GTTTATGGAT 
    ACTGAATTGA CTAGCATGTC CGATGTTTTT GAAGCAAAAG AAAGCAAATT TTTAGAAAAA 
    AAAATTTTAA GACTTGCTAG AGATGTTATT GAGAGACTTG ATTTAGATAT ATTTACGTCT 
    ATCCAAACTA AAAGGCCTTT ACGCCAACTC GCTTTACTTG CCTGTGGATT AGGAATGTCA 
    AATGATGACT TGTTGTTGGA AATTAGAAGA CTGATTCGCA AGAATGAGCA TGTAAAAGCC 
    GCTGGGTTAG CACTTTTCCA CGGAAAAATT GAAAACGTAG TCCGTATATT ATCAAGTGGG 
    AATGAGCTGG AGAAGACCAT ATCCACAGCA GTTGCCGGAT ACATTACATC GCAAGGCTTA 
    AGTAACTTTG GAAGTGACTC ATTGTGGAAA GAAATGTCTC GTAACTTAAG TACAGAATTG 
    GAAGATCCAT ACTTACGTGC AATATTTGCG TATGTTTCCA ACTCCGATTG GAGAGATGTA 
    TTAGATGAAG TTTCTCTCTC ATTAAAAGAT CGTCTTGGAA TTGCTCTTCG CTTTTTACCA 
    GATGACGATT TGAGTAATTA TTTGTGCGAT TTATGTCACA CTACAGTGCA ATCAGGCGAC 
    CCAGAAGGCC TGTTACTTAC AGGTTTAACT CCTTTGGGAA TGGAACTTCT GCAGAACTAC 
    ATTGATCATA CAAGCGATGT TCAAACTGCT GCATTGATTG CTGCATTTGT GGTACCTAAG 
    AAATTTCTGG ATAAACGTGC AGAGGATTGG ACTGAAAGTT ATAGAGAGCT TCTAAACCGC 
    TGGAAACTTT ACCGTGAGCG TGCAAAGTTT GATATCTTTC GGACAGAACT CTCAAAAAAT 
    CACACAGGAG AAATAACCAG AAAGGCTACA GAGCCATCTA TACGCATTAT CTGCAATTTT 
    TGTAGAAAAC CAATTTTTCC TTTCTCCAAT AGGAATGAAT GTAATAATTT ACCAACGCCA 
    ATTCAACGAG GTGTCAGTAA AGCAGGCCCA GCAAAACACT TGGGAAAAAG TTGTCCTCAT 
    TGCGGACAGC CTCTTCCAAA GTGCTCTGTA TGTGGGTTTT CTTTAGGAGA TGAAGATGTT 
    CCTCAGAAAG ATGACTTTTC TCAAAAACCT CAAAACTATG TCAAAGAAGT TAATCTTCAG 
    AAATCTAGAT TTGGGTTATG GTTTAGCTTT TGTCTAAATT GTGGCCATGG AGCTCACGCA 
    TCTCATGCTT CAGAATGGTT TTCAACTCAT ACAATATGTC CGGTTCCTAA CTGTGATTGT 
    GAATGCAAAC TGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342800.1
    CDS401..3118
    Translation

    Target ORF information:

    RefSeq Version NM_001355976.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe ubiquitin-protein ligase E3 (SPAC12G12.01c), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001355976.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTTAAATA GAAGTAAAAA GAGGAATGGC TGTTATTTTT GGGTTAACGG CTCCTCAGAT 
    GAGATTCGAT ATGTCGCCGT CAAAGCTTCC CCTAAAGTAA ATTGGAAAAC TCACATTATT 
    TGGAGATCAT TGAAAAATGT TAAATGTATA GACTCTTTTC ACGGAAACAA TGAAATATTG 
    GGTGCTGGTT CTTCTACCGG AAATATATCA TTGCTTTCTG TTAAGCATCC AGAATTTCAA 
    GCAGTAGTAA CACCTGGATA TGCTCGCCCT TGCAATAGTT TGGCATTTTC TGAAACTGAA 
    CATCTTGTTG CTGGATTTGC AAAATCTCGA AACGAGTCTT CCTTGAAGTT ATGGGACTTG 
    AATTCATTAC TGTCTGACCC GAAGAGTAGT CCCTTAATGC AGTCTTCTAC TCTAGACGGA 
    GTTTCTTCCG TTTGTTATAA GAAAGACACT CCTCTTTTGC TTACCGGATC AACTTCGCGT 
    TCTGTCCATA TAATTGATAC TAGGCAGCAA CTAGACTCAG TTTCTTCCGT CAATACGCAA 
    TATTATAGTA ATATAGTTGT TGATCCATTT TCTCCTAACT ATTTTGCCGC AAATTCTTAT 
    GATGGTGACA TTGCGATTTT TGATACTCGA TACTTCAAAT CCGATAATTA TTTACAAATT 
    ATTTTACGGA ACGAAAATAA AAAGCCCAAA AATCCACAAT TATTTGCACT GAAATATTCT 
    GAATGGAAAC CTGGACAATT GGCTGTTTTG TCAAACAATT CGATTACACT TCGTCAATTA 
    TTGCCTTGTG TCAATGGAAA TGAAGGATCC GCAAATAATT CTGTCTTTGT AAATTATGAG 
    AAGAAATATC CTGTTAAACC AAATTCACAA TGTTCTGGCA TTGATTTTTT TACTCCTTCT 
    ACAGCCTTTC CAACTCATGT TCAAATTTTG GGTGTCATAA ACGAACAACC GAAACTTTTT 
    TCTGTCCATG ATGAGGTCAT TCCCTTTTCA TTTAACCCTT ATAATGATTT GATATTTTCC 
    TTCAAAGAAA AACTGTATCC TTTAAACTCT TCACCATTTA ACACACTTTC TGATGTCCCA 
    CAATTTGATG TCAGCGAATT TGTCGATGAA AACTCTTTCG ATTCCTCGTC TTCTTGTTCC 
    TCCAAAGTCT TTCTAACGAC AAGAAACAAC TCAATCAATT CAGAGGATTC CGCTCATGAA 
    GTATTGCTTT CCTATAACCG CGTCCTTGGA TCTGATATTC AAGGGACAAT ACTAGATCGC 
    GTCAAAAAGG GCTACCAATT TGATTCCCAA AAAAACTCAG AATTAGTTTC TGATTTATAC 
    TTAAAAGATC TATGGTCTTG GATTCACTTA AGCCATAGAC AATCAGAAGA ATCACTGTTT 
    GGAGACACAG GAGATACAGA TTTCAGCTAT CAAGGTGCAC TAGGTATTTG GTTTATGGAT 
    ACTGAATTGA CTAGCATGTC CGATGTTTTT GAAGCAAAAG AAAGCAAATT TTTAGAAAAA 
    AAAATTTTAA GACTTGCTAG AGATGTTATT GAGAGACTTG ATTTAGATAT ATTTACGTCT 
    ATCCAAACTA AAAGGCCTTT ACGCCAACTC GCTTTACTTG CCTGTGGATT AGGAATGTCA 
    AATGATGACT TGTTGTTGGA AATTAGAAGA CTGATTCGCA AGAATGAGCA TGTAAAAGCC 
    GCTGGGTTAG CACTTTTCCA CGGAAAAATT GAAAACGTAG TCCGTATATT ATCAAGTGGG 
    AATGAGCTGG AGAAGACCAT ATCCACAGCA GTTGCCGGAT ACATTACATC GCAAGGCTTA 
    AGTAACTTTG GAAGTGACTC ATTGTGGAAA GAAATGTCTC GTAACTTAAG TACAGAATTG 
    GAAGATCCAT ACTTACGTGC AATATTTGCG TATGTTTCCA ACTCCGATTG GAGAGATGTA 
    TTAGATGAAG TTTCTCTCTC ATTAAAAGAT CGTCTTGGAA TTGCTCTTCG CTTTTTACCA 
    GATGACGATT TGAGTAATTA TTTGTGCGAT TTATGTCACA CTACAGTGCA ATCAGGCGAC 
    CCAGAAGGCC TGTTACTTAC AGGTTTAACT CCTTTGGGAA TGGAACTTCT GCAGAACTAC 
    ATTGATCATA CAAGCGATGT TCAAACTGCT GCATTGATTG CTGCATTTGT GGTACCTAAG 
    AAATTTCTGG ATAAACGTGC AGAGGATTGG ACTGAAAGTT ATAGAGAGCT TCTAAACCGC 
    TGGAAACTTT ACCGTGAGCG TGCAAAGTTT GATATCTTTC GGACAGAACT CTCAAAAAAT 
    CACACAGGAG AAATAACCAG AAAGGCTACA GAGCCATCTA TACGCATTAT CTGCAATTTT 
    TGTAGAAAAC CAATTTTTCC TTTCTCCAAT AGGAATGAAT GTAATAATTT ACCAACGCCA 
    ATTCAACGAG GTGTCAGTAA AGCAGGCCCA GCAAAACACT TGGGAAAAAG TTGTCCTCAT 
    TGCGGACAGC CTCTTCCAAA GTGCTCTGTA TGTGGGTTTT CTTTAGGAGA TGAAGATGTT 
    CCTCAGAAAG ATGACTTTTC TCAAAAACCT CAAAACTATG TCAAAGAAGT TAATCTTCAG 
    AAATCTAGAT TTGGGTTATG GTTTAGCTTT TGTCTAAATT GTGGCCATGG AGCTCACGCA 
    TCTCATGCTT CAGAATGGTT TTCAACTCAT ACAATATGTC CGGTTCCTAA CTGTGATTGT 
    GAATGCAAAC TGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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