glt1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	glt1
Entrez Gene ID	2543509
Full Name	glutamate synthase Glt1 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019557.2	NP_594133.1	putative glutamate synthase Glt1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG9674	NP_001246804.1
Schizosaccharomyces pombe (fission yeast)	glt1	NP_594133.1
Magnaporthe oryzae (rice blast fungus)	MGG_07187	XP_003715392.1
Neurospora crassa	NCU01744	XP_957167.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006360	XP_316385.4
Caenorhabditis elegans (roundworm)	W07E11.1	NP_509693.2
Kluyveromyces lactis	KLLA0E19625g	XP_454839.1
Eremothecium gossypii	AGOS_ADR290W	NP_984386.2
Arabidopsis thaliana (thale cress)	GLT1	NP_200158.2
Oryza sativa (rice)	Os01g0681900	NP_001043884.1
Saccharomyces cerevisiae (baker's yeast)	GLT1	NP_010110.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following glt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the glt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102964	NM_001019557.2 Latest version!	Schizosaccharomyces pombe putative glutamate synthase Glt1 (glt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

glt1 ( NM_001019557.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594133.1 Schizosaccharomyces pombe putative glutamate synthase Glt1 (glt1), mRNA.

Price & Availability↑

CloneID	OSc102964
Clone ID Related Accession (Same CDS sequence)	NM_001019557.2
Accession Version	NM_001019557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative glutamate synthase Glt1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019557.1.

ATGGCTGTCT TATCTTCAGT CCAACCCATC AATCATAACT CTGCCCTCGT TGAGGCTCGT 
GACGAACAAG TGAACACGAC CGCTTGTTCC GATGACCTTC TCAATGCTCC TCCCTATGAG 
TATGATACTG AGGGAAATCC CTCTTGGGCT GGTGCCCTTC CCAAGGCTCA AGCTTTATAC 
GATCCTGCCT ACGAAAAAGA CTCTTGCGGT GTTGGTTTCA CTTGTCACAT CAAGGGTCAA 
GTCTCTCACA AAATCGTCAC CGATGCTCGC TTACTTTTGT GCAACATGAC TCATCGTGGT 
GCCACTGGTG CCGATACTCG TGATGGTGAT GGTGCAGGTG TCATGACCGG TATGCCTTAC 
ACTTTTATGC AAAAGGAGTT TGGTCAAATC GGATGTACCC TTCCCAAAAG CGGCGAGTAT 
GCCATCGGTA ATGTGTTTTT CTCTCCTGAA GCCGACGTTT GTCGTGAAGC CATGACCGCT 
TTCACCCAAG TCGCCGAAAA ACTTGGTCTT GCAATCCTTG CTTGGCGTTC CGTCCCTTGT 
GATAACAGCA TCTTGGGCCC CGCTGCTCTT TCTCGTGAGC CTACCATCTT ACAGCCTTGT 
GTTGTCCTCA AGGCCGCTTA CGATGGTGAA GCCGAGTTTG ATACCGATCT CTTCGAAAGA 
CAGCTTTACG TTCTTCGCAA GCAGTCTTCT CATTTGATCG GTAAGGAAAA ATGGTTCTAC 
ATTTGCTCTT TGCATCGTGA GACCATTGTC TATAAGGGTC AACTTGCTCC TGTTCAAGTC 
TACAATTATT TCCTTGATTT GAACAACGCC GAGTATGTAT CCCACTTTGC CCTCGTTCAC 
TCCCGTTTCT CTACAAACAC TTTCCCGTCC TGGGACCGTG CTCAACCCAT GAGATTGGCC 
GCTCACAACG GTGAAATTAA CACCCTACGT GGTAACAAAA ATTGGATGCA TGCTCGTGAA 
GGCTTGATGA AGTCCAGTCG TTTTGGCGAA GAGTTCGCCT CTTTGCTTCC CATCATCGAG 
CGCGGTGGTT CGGACTCCGC TGCTTTTGAT AACGTTATTG AGTTGTTATG TGCTAGCGGT 
GTTGTCTCCT TACCCGAAGC TGTCATGCTC TTGATTCCTG AAGCTTGGCA AAACGACAAA 
AACATCAGCG ACGAGAAGGC TGCTTTTTAC GAGTGGGCTG CTTGTCAAAT GGAGCCTTGG 
GATGGTCCTG CTTTGTTTAC ATTTGCCGAC GGTCGCTATT GCGGTGCCAA TCTTGATCGT 
AACGGTCTTC GCCCTTGCCG TTTCTATCTT ACTTCCGATG ACATGATGAT TTGCGCTTCT 
GAAGTTGGTA CAGTCGGCAT TGAACCTGAC CGCATCGTTC AGAAAGGTCG TCTATATCCA 
GGCCGTATGT TGCTTGTTGA CACCAAAGAG GGTCGTATTG TCGACGATAA GGAGCTCAAG 
CACAACATTG CCAGTCGCTA CGACTTCCGT AGTTGGCTCG ACCAAGAGCT TATCGACATG 
AACTCGATTG TTGACTCTCT TATAGAATCC ACTAGCGTTG ATCTTACTCC CATTGTCGAT 
GATGTTCCTC TTGCCGATGA CAAAACCATG TTAGCATTTG GTTACACTTT GGAACAGATT 
AACATGATTA TGGCTCCCAT GGCTAATGGC GGCAAGGAAA CTTTGGGAAG TATGGGTAAT 
GATGCTGCAA TCGCATGCCT TTCCGACCAG CCTCGTTTAC TTTACGACTA TTTCCGTCAA 
CTTTTCGCTC AAGTCACTAA TCCTCCTATT GATCCTATTC GTGAGGCTAT CGTAATGAGT 
TTGCAATGCT ACATCGGTCC TAGTGGTAAC TTGCTTGAAA TTAATCAAAG CCAATGTCGT 
CGTCTCCGCA TGCCTACTCC CATTTTGACT GTTGAGGAGT TCAATGCTTT GAAAAATGTT 
GACCGTATTT ACCCTGACTG GAAAGTCGCT TCTATCGACA TCACTTTTTT CAAGAGTGAA 
GGCGTTGCTG GCTATGCTGC CGCCATCGAG CGTATTTGTT CCGAAGCTGA TACTGCCGTC 
AATGAGGGCT ATAAAGCTAT AGTCCTTTCC GACCGTAACG TTAACAGTGA GCGCGTACCT 
TTGGCTTCCA TTGCCGCATG CGGTGCCGTT CATCATTATT TGGTACAAAA CAAATTGCGT 
TCTAGAGTTG CTCTTGTTTG TGAATCTGGT GACGCGCGTG AAGTTCACCA TATGTGCACA 
CTTCTTGGCT ATGGTGCCGA TGCCGTTTGT CCCTATTTAG CCATGGAAGC TTTAACAAAG 
CTAGTCAGAC AGAACGCAAT GAAACCTGGC ATCACAGAAG AAACTGCCAT AAAGAATTTC 
AAGCATGCTA TCAATGGCGG TATTTTGAAG GTTATGTCCA AGATGGGTAT TTCAACTTTG 
CAATCATATA AAGGTGCCCA GATTTTTGAG GCTCTTGGCA TTGACAACGA GGTCATCAAC 
AAATGTTTCC TTGGTACCGC ATCCCGTATC CGTGGTGTAA CTTTTGAGCA TATTGCTCTT 
GATGCTTTTG CTCTTCATGA GCGTGGATAT CCAACCGATC AATCCATTCG CAGTCTTCAA 
ATCCCCGATA TGGGTGACTT TTACTATCGT GATGGTGGTG AGCAGCACGT CAACCATCCA 
AAGGCTATCG CCAGTCTTCA AGATGCCGTC CGCAATAAGA ACGAGGCTGC TTATGCGGAA 
TTCTCTCGTA CACACTACGA ACAAACTAGG AGGTGTACTC TTCGTGGTAT GTTGGATTTC 
GATTTTGATT CTTCTCAAGC TATTCCCATT GAACAGGTTG AGCCTTGGAC TGAGATTGTT 
CGCCGTTTCT GCACTGGTGC TATGTCTTAC GGTTCGATCA GTATGGAATC TCATTCTTCT 
TTGGCCATTG CCATGAATCG TTTGGGCGGT AAGTCTAACA CTGGTGAAGG TGGTGAAGAC 
CCTGCTCGCA GTCAGCGTCT TGCCAATGGC GATACAATGC GTTCTGCCAT CAAACAGATT 
GCATCCGGTC GTTTCGGTGT TACTTCTTGG TACTTGTCTG ATGCTGATGA GCTTCAAATT 
AAAATGGCTC AAGGTGCCAA GCCTGGTGAG GGTGGTGAAT TGCCTGGTAA TAAGGTGAGT 
GAATCAATTG CGAAAACTCG TCATTCCACT GCTGGTGTTG GTTTGATTTC TCCTCCTCCT 
CATCATGATA TCTACTCTAT TGAAGATTTG AAGCAGTTGA TTTATGACAT GAAGAGTGCC 
AACCCTCGTG CTCGTGTATC CGTTAAGCTT GTTTCTGAAG TAGGTGTCGG TATTGTTGCT 
TCTGGTGTTG CAAAAGCCAA AGCAGATCAT ATTTTGGTTT CAGGTCATGA TGGTGGTACC 
GGTGCCTCTC GTTGGACTGG TATTAAATAC GCTGGTTTAC CATGGGAGCT AGGTGTTGCC 
GAAACCCATC AAACTTTGGT ATTGAACGAC CTTCGTGGGC GAGTTGTTAT CCAAACTGAT 
GGTCAAATTC GTACCGGTCG TGATGTTGCT ATCGCTTGCT TGCTTGGTGC TGAAGAATGG 
GGGTTTGCGA CTACTCCTTT GATTGCATTG GGCTGTATCA TGATGCGTAA ATGTCATCTG 
AATACTTGTC CCGTTGGTAT CGCAACTCAA GATCCTGAAT TGAGGAAGAA ATTTGAGGGT 
CAACCGGAGC ACGTTGTAAA CTTTTTCTAC TACGTCGCAG AAGAGCTCCG TGGAATTATG 
GCTAAACTTG GTTTCCGAAC TATTAATGAG ATGGTTGGTC GTTCTGACAA GCTTAAGGTC 
GCCGAGCCAA TTAACAACAA GTCAAAGCTT CTTGACTTGA CTCCCTTGCT GACTCCTGCT 
TTCACTCTTC GACCTGGCGC TGCTACTTAC AACGTTCGTA AGCAAGACCA TAGACTTTAT 
ACCAGACTTG ACAACAAGCT CATTGACGAA GCTGAAGTTA CTTTGGAAGA AGGCATCCCT 
TCTGTCGTTG AATGTGAAAT TATTAACACC GATCGTACTC TTGGTGCTAC TCTTTCCAAT 
AAGATCAGTA AGCGTTATGG TGAAGAGGGT CTTCCTACTG ATTCTATTCG TGTCAATGTT 
TTTGGTTCAG CTGGCCAATC TTTTGGTGCT TTCTTAGCTC CAGGTGTTAC ACTCCAATTA 
GAAGGTGACT GTAACGATTA TGTCGGCAAA GGCTTATCTG GCGGTCGCTT GATTATTTAT 
CCTCCTAGGG TTTCTCCCTT TAAGCCCGAA GAAAATATGA TCATTGGTAA TGTCTGTCTG 
TATGGTGCCA CTTCTGGTCA TGCTTTTATT TCTGGTGTGG CAGCAGAGCG TTTCGCTGTT 
CGTAACTCTG GTGCAATTGC TGTAGTTGAA GGCGTTGGTG ACCACGGTTG TGAGTACATG 
ACTGGTGGTC GTGTTGTAAT TCTTGGTTCA ACTGGCAGAA ACTTCGCTGC TGGTATGTCT 
GGTGGTATTG CATATGTCTA CGATATGCAA ATGGACTTTG CTGGTAAAAT TAACACAGAA 
ATGGTCGATA TTTCTTCTGT CACTGATGCT GCTGAAATTG CTTTCCTCAG AGGACTTATT 
CAAGACCATA GGCATTACAC GGGCTCACAG GTGGCTGACC GTATATTAAG TGATTTCCCT 
CGTCACCTTA GCCGCTTTGT TAAGGTTCTT CCAAGAGAGT ACAAGGCTGT ATTGGAACGT 
GAAGCTGCTA AGAAGGAAGA AGCCAAGAGA CTACAGTATC CCAAAGCTTT CATGCCAGGA 
AATCCTATTC GTCAACAAAT TGAGGAAACT AATGCTCAGA TTGCTGATGT GGAAGATACC 
TTAGGTGCTA CTGTGAAAAA GAGCGCTCCT TTGGACAAGC TTCGTGGATT TATGAAATAC 
CAACGCCGCA GTGAACATTA TCGTAACCCT CTTAAGAGAA CGAATGATTG GAAAGAACTT 
TCCGTTCGTC TTCGTGAAGA TGAATTACGT GTTCAAACTG CCAGATGTAT GGACTGTGGT 
ACTCCTTTCT GTCAATCTGA TTATGGTTGT CCTATTTCCA ACAAAATTTT CACTTGGAAT 
GATTTGGTTT TCAAACAACA GTGGAAAGAG GCCTTAACCC AGCTTTTGTT GACCAATAAT 
TTTCCAGAAT TTACAGGCCG TGTCTGCCCT GCTCCTTGTG AAGGCGCTTG TACCTTAGGT 
ATTATTGAAT CGCCTGTTGG AATCAAGAGT GTTGAGCGTG CTATTATTGA CAAGGCTTGG 
GAGGAAGGCT GGATTGTCCC CAGACCTCCT GCTGAACGTA CTGGTCGTCG TGTTGCCATT 
ATCGGTTCTG GGCCTGCAGG TCTTGCTGCC GCTGACCAAC TCAACCGCGC TGGTCACCAT 
GTAGTAATTT ATGAGCGTGC CGATCGTCCA GGTGGTCTTC TTCAATATGG TATTCCTAAT 
ATGAAACTAG ACAAGAAGGT TGTTGAGCGT CGTATCCAGC TCATGATTGA CGAAGGTATT 
GAAGTACTTA CAAACGTTGA AGTCGGTAAG AACGGTGATG TTAGCCTTGA TGAACTTCAC 
AAGGTTTACG ATGCTGTTGT TCTCGCCTCT GGCTCTACGG TTCCTCGTGA TTTGCCCATT 
CCCAACCGTG ATTCTAAGGG TATTCACTTT GCCATGGAGT TCCTTCACAA AAATACTAAG 
AGTCTTCTTG ATAGTGAACT CAAAGATGGC AATTATATTA GTGCTAAGGG CAAGGATGTA 
ATTGTTATTG GAGGAGGTGA TACTGGTAAT GATTGTTTGG GTACCAGTGT TCGTCATGGT 
GCTAAAAGTG TTAGAAATTT GGAGTTACTT CCCATTCCTC CTCGTGAACG TGCCTTCGAC 
AACCCTTGGC CTCAATACCC TCGTGTCTTC CGCGTTGATT ATGGACATGC TGAAGTACAA 
GCTCATTATG GTCAAGATTT CCGTGAATAC TCTATTTTAA CTAAGAGTTT TGAAAAGGAT 
GAAGATGGCA ATGTTAAGGG TATTAATACT GTTCGTATTG AGTGGACTAA GAACAGTAAG 
GGTCGCTGGA TCATGAAGGA GATTCGTAAC TCTGAGGAGT TTTTCCCTGC AGATTTAGTC 
ATCCTTGCTT TAGGTTTCTT AGGTCCTGAG GAGCAAGCTA CTGCTGGAAT GAACGTTGAT 
AGAGATGCTC GTTCTAACAT CAGCACTCCT ACTAAATCGT ATGAAACTAG TGTTCCTGGC 
ATTTACGCTG CTGGTGATTG CCGCCGTGGT CAAAGTTTGG TCGTATGGGG TATTCAAGAA 
GGTCGTCAAT GTGCTCGTGA AATTGATTTG AAATTCCAGG GTAAGACATT TTTGCCTGGT 
GATGGAGGCC TTGTAAAGCG CACTGTTAAT TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594133.1
CDS	756..7091
Translation	MAVLSSVQPINHNSALVEARDEQVNTTACSDDLLNAPPYEYDTEGNPSWAGALPKAQALYDPAYEKDSCGVGFTCHIKGQVSHKIVTDARLLLCNMTHRGATGADTRDGDGAGVMTGMPYTFMQKEFGQIGCTLPKSGEYAIGNVFFSPEADVCREAMTAFTQVAEKLGLAILAWRSVPCDNSILGPAALSREPTILQPCVVLKAAYDGEAEFDTDLFERQLYVLRKQSSHLIGKEKWFYICSLHRETIVYKGQLAPVQVYNYFLDLNNAEYVSHFALVHSRFSTNTFPSWDRAQPMRLAAHNGEINTLRGNKNWMHAREGLMKSSRFGEEFASLLPIIERGGSDSAAFDNVIELLCASGVVSLPEAVMLLIPEAWQNDKNISDEKAAFYEWAACQMEPWDGPALFTFADGRYCGANLDRNGLRPCRFYLTSDDMMICASEVGTVGIEPDRIVQKGRLYPGRMLLVDTKEGRIVDDKELKHNIASRYDFRSWLDQELIDMNSIVDSLIESTSVDLTPIVDDVPLADDKTMLAFGYTLEQINMIMAPMANGGKETLGSMGNDAAIACLSDQPRLLYDYFRQLFAQVTNPPIDPIREAIVMSLQCYIGPSGNLLEINQSQCRRLRMPTPILTVEEFNALKNVDRIYPDWKVASIDITFFKSEGVAGYAAAIERICSEADTAVNEGYKAIVLSDRNVNSERVPLASIAACGAVHHYLVQNKLRSRVALVCESGDAREVHHMCTLLGYGADAVCPYLAMEALTKLVRQNAMKPGITEETAIKNFKHAINGGILKVMSKMGISTLQSYKGAQIFEALGIDNEVINKCFLGTASRIRGVTFEHIALDAFALHERGYPTDQSIRSLQIPDMGDFYYRDGGEQHVNHPKAIASLQDAVRNKNEAAYAEFSRTHYEQTRRCTLRGMLDFDFDSSQAIPIEQVEPWTEIVRRFCTGAMSYGSISMESHSSLAIAMNRLGGKSNTGEGGEDPARSQRLANGDTMRSAIKQIASGRFGVTSWYLSDADELQIKMAQGAKPGEGGELPGNKVSESIAKTRHSTAGVGLISPPPHHDIYSIEDLKQLIYDMKSANPRARVSVKLVSEVGVGIVASGVAKAKADHILVSGHDGGTGASRWTGIKYAGLPWELGVAETHQTLVLNDLRGRVVIQTDGQIRTGRDVAIACLLGAEEWGFATTPLIALGCIMMRKCHLNTCPVGIATQDPELRKKFEGQPEHVVNFFYYVAEELRGIMAKLGFRTINEMVGRSDKLKVAEPINNKSKLLDLTPLLTPAFTLRPGAATYNVRKQDHRLYTRLDNKLIDEAEVTLEEGIPSVVECEIINTDRTLGATLSNKISKRYGEEGLPTDSIRVNVFGSAGQSFGAFLAPGVTLQLEGDCNDYVGKGLSGGRLIIYPPRVSPFKPEENMIIGNVCLYGATSGHAFISGVAAERFAVRNSGAIAVVEGVGDHGCEYMTGGRVVILGSTGRNFAAGMSGGIAYVYDMQMDFAGKINTEMVDISSVTDAAEIAFLRGLIQDHRHYTGSQVADRILSDFPRHLSRFVKVLPREYKAVLEREAAKKEEAKRLQYPKAFMPGNPIRQQIEETNAQIADVEDTLGATVKKSAPLDKLRGFMKYQRRSEHYRNPLKRTNDWKELSVRLREDELRVQTARCMDCGTPFCQSDYGCPISNKIFTWNDLVFKQQWKEALTQLLLTNNFPEFTGRVCPAPCEGACTLGIIESPVGIKSVERAIIDKAWEEGWIVPRPPAERTGRRVAIIGSGPAGLAAADQLNRAGHHVVIYERADRPGGLLQYGIPNMKLDKKVVERRIQLMIDEGIEVLTNVEVGKNGDVSLDELHKVYDAVVLASGSTVPRDLPIPNRDSKGIHFAMEFLHKNTKSLLDSELKDGNYISAKGKDVIVIGGGDTGNDCLGTSVRHGAKSVRNLELLPIPPRERAFDNPWPQYPRVFRVDYGHAEVQAHYGQDFREYSILTKSFEKDEDGNVKGINTVRIEWTKNSKGRWIMKEIRNSEEFFPADLVILALGFLGPEEQATAGMNVDRDARSNISTPTKSYETSVPGIYAAGDCRRGQSLVVWGIQEGRQCAREIDLKFQGKTFLPGDGGLVKRTVNC

Target ORF information:

RefSeq Version	NM_001019557.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative glutamate synthase Glt1 (glt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019557.2

ORF Insert Sequence:

ATGGCTGTCT TATCTTCAGT CCAACCCATC AATCATAACT CTGCCCTCGT TGAGGCTCGT 
GACGAACAAG TGAACACGAC CGCTTGTTCC GATGACCTTC TCAATGCTCC TCCCTATGAG 
TATGATACTG AGGGAAATCC CTCTTGGGCT GGTGCCCTTC CCAAGGCTCA AGCTTTATAC 
GATCCTGCCT ACGAAAAAGA CTCTTGCGGT GTTGGTTTCA CTTGTCACAT CAAGGGTCAA 
GTCTCTCACA AAATCGTCAC CGATGCTCGC TTACTTTTGT GCAACATGAC TCATCGTGGT 
GCCACTGGTG CCGATACTCG TGATGGTGAT GGTGCAGGTG TCATGACCGG TATGCCTTAC 
ACTTTTATGC AAAAGGAGTT TGGTCAAATC GGATGTACCC TTCCCAAAAG CGGCGAGTAT 
GCCATCGGTA ATGTGTTTTT CTCTCCTGAA GCCGACGTTT GTCGTGAAGC CATGACCGCT 
TTCACCCAAG TCGCCGAAAA ACTTGGTCTT GCAATCCTTG CTTGGCGTTC CGTCCCTTGT 
GATAACAGCA TCTTGGGCCC CGCTGCTCTT TCTCGTGAGC CTACCATCTT ACAGCCTTGT 
GTTGTCCTCA AGGCCGCTTA CGATGGTGAA GCCGAGTTTG ATACCGATCT CTTCGAAAGA 
CAGCTTTACG TTCTTCGCAA GCAGTCTTCT CATTTGATCG GTAAGGAAAA ATGGTTCTAC 
ATTTGCTCTT TGCATCGTGA GACCATTGTC TATAAGGGTC AACTTGCTCC TGTTCAAGTC 
TACAATTATT TCCTTGATTT GAACAACGCC GAGTATGTAT CCCACTTTGC CCTCGTTCAC 
TCCCGTTTCT CTACAAACAC TTTCCCGTCC TGGGACCGTG CTCAACCCAT GAGATTGGCC 
GCTCACAACG GTGAAATTAA CACCCTACGT GGTAACAAAA ATTGGATGCA TGCTCGTGAA 
GGCTTGATGA AGTCCAGTCG TTTTGGCGAA GAGTTCGCCT CTTTGCTTCC CATCATCGAG 
CGCGGTGGTT CGGACTCCGC TGCTTTTGAT AACGTTATTG AGTTGTTATG TGCTAGCGGT 
GTTGTCTCCT TACCCGAAGC TGTCATGCTC TTGATTCCTG AAGCTTGGCA AAACGACAAA 
AACATCAGCG ACGAGAAGGC TGCTTTTTAC GAGTGGGCTG CTTGTCAAAT GGAGCCTTGG 
GATGGTCCTG CTTTGTTTAC ATTTGCCGAC GGTCGCTATT GCGGTGCCAA TCTTGATCGT 
AACGGTCTTC GCCCTTGCCG TTTCTATCTT ACTTCCGATG ACATGATGAT TTGCGCTTCT 
GAAGTTGGTA CAGTCGGCAT TGAACCTGAC CGCATCGTTC AGAAAGGTCG TCTATATCCA 
GGCCGTATGT TGCTTGTTGA CACCAAAGAG GGTCGTATTG TCGACGATAA GGAGCTCAAG 
CACAACATTG CCAGTCGCTA CGACTTCCGT AGTTGGCTCG ACCAAGAGCT TATCGACATG 
AACTCGATTG TTGACTCTCT TATAGAATCC ACTAGCGTTG ATCTTACTCC CATTGTCGAT 
GATGTTCCTC TTGCCGATGA CAAAACCATG TTAGCATTTG GTTACACTTT GGAACAGATT 
AACATGATTA TGGCTCCCAT GGCTAATGGC GGCAAGGAAA CTTTGGGAAG TATGGGTAAT 
GATGCTGCAA TCGCATGCCT TTCCGACCAG CCTCGTTTAC TTTACGACTA TTTCCGTCAA 
CTTTTCGCTC AAGTCACTAA TCCTCCTATT GATCCTATTC GTGAGGCTAT CGTAATGAGT 
TTGCAATGCT ACATCGGTCC TAGTGGTAAC TTGCTTGAAA TTAATCAAAG CCAATGTCGT 
CGTCTCCGCA TGCCTACTCC CATTTTGACT GTTGAGGAGT TCAATGCTTT GAAAAATGTT 
GACCGTATTT ACCCTGACTG GAAAGTCGCT TCTATCGACA TCACTTTTTT CAAGAGTGAA 
GGCGTTGCTG GCTATGCTGC CGCCATCGAG CGTATTTGTT CCGAAGCTGA TACTGCCGTC 
AATGAGGGCT ATAAAGCTAT AGTCCTTTCC GACCGTAACG TTAACAGTGA GCGCGTACCT 
TTGGCTTCCA TTGCCGCATG CGGTGCCGTT CATCATTATT TGGTACAAAA CAAATTGCGT 
TCTAGAGTTG CTCTTGTTTG TGAATCTGGT GACGCGCGTG AAGTTCACCA TATGTGCACA 
CTTCTTGGCT ATGGTGCCGA TGCCGTTTGT CCCTATTTAG CCATGGAAGC TTTAACAAAG 
CTAGTCAGAC AGAACGCAAT GAAACCTGGC ATCACAGAAG AAACTGCCAT AAAGAATTTC 
AAGCATGCTA TCAATGGCGG TATTTTGAAG GTTATGTCCA AGATGGGTAT TTCAACTTTG 
CAATCATATA AAGGTGCCCA GATTTTTGAG GCTCTTGGCA TTGACAACGA GGTCATCAAC 
AAATGTTTCC TTGGTACCGC ATCCCGTATC CGTGGTGTAA CTTTTGAGCA TATTGCTCTT 
GATGCTTTTG CTCTTCATGA GCGTGGATAT CCAACCGATC AATCCATTCG CAGTCTTCAA 
ATCCCCGATA TGGGTGACTT TTACTATCGT GATGGTGGTG AGCAGCACGT CAACCATCCA 
AAGGCTATCG CCAGTCTTCA AGATGCCGTC CGCAATAAGA ACGAGGCTGC TTATGCGGAA 
TTCTCTCGTA CACACTACGA ACAAACTAGG AGGTGTACTC TTCGTGGTAT GTTGGATTTC 
GATTTTGATT CTTCTCAAGC TATTCCCATT GAACAGGTTG AGCCTTGGAC TGAGATTGTT 
CGCCGTTTCT GCACTGGTGC TATGTCTTAC GGTTCGATCA GTATGGAATC TCATTCTTCT 
TTGGCCATTG CCATGAATCG TTTGGGCGGT AAGTCTAACA CTGGTGAAGG TGGTGAAGAC 
CCTGCTCGCA GTCAGCGTCT TGCCAATGGC GATACAATGC GTTCTGCCAT CAAACAGATT 
GCATCCGGTC GTTTCGGTGT TACTTCTTGG TACTTGTCTG ATGCTGATGA GCTTCAAATT 
AAAATGGCTC AAGGTGCCAA GCCTGGTGAG GGTGGTGAAT TGCCTGGTAA TAAGGTGAGT 
GAATCAATTG CGAAAACTCG TCATTCCACT GCTGGTGTTG GTTTGATTTC TCCTCCTCCT 
CATCATGATA TCTACTCTAT TGAAGATTTG AAGCAGTTGA TTTATGACAT GAAGAGTGCC 
AACCCTCGTG CTCGTGTATC CGTTAAGCTT GTTTCTGAAG TAGGTGTCGG TATTGTTGCT 
TCTGGTGTTG CAAAAGCCAA AGCAGATCAT ATTTTGGTTT CAGGTCATGA TGGTGGTACC 
GGTGCCTCTC GTTGGACTGG TATTAAATAC GCTGGTTTAC CATGGGAGCT AGGTGTTGCC 
GAAACCCATC AAACTTTGGT ATTGAACGAC CTTCGTGGGC GAGTTGTTAT CCAAACTGAT 
GGTCAAATTC GTACCGGTCG TGATGTTGCT ATCGCTTGCT TGCTTGGTGC TGAAGAATGG 
GGGTTTGCGA CTACTCCTTT GATTGCATTG GGCTGTATCA TGATGCGTAA ATGTCATCTG 
AATACTTGTC CCGTTGGTAT CGCAACTCAA GATCCTGAAT TGAGGAAGAA ATTTGAGGGT 
CAACCGGAGC ACGTTGTAAA CTTTTTCTAC TACGTCGCAG AAGAGCTCCG TGGAATTATG 
GCTAAACTTG GTTTCCGAAC TATTAATGAG ATGGTTGGTC GTTCTGACAA GCTTAAGGTC 
GCCGAGCCAA TTAACAACAA GTCAAAGCTT CTTGACTTGA CTCCCTTGCT GACTCCTGCT 
TTCACTCTTC GACCTGGCGC TGCTACTTAC AACGTTCGTA AGCAAGACCA TAGACTTTAT 
ACCAGACTTG ACAACAAGCT CATTGACGAA GCTGAAGTTA CTTTGGAAGA AGGCATCCCT 
TCTGTCGTTG AATGTGAAAT TATTAACACC GATCGTACTC TTGGTGCTAC TCTTTCCAAT 
AAGATCAGTA AGCGTTATGG TGAAGAGGGT CTTCCTACTG ATTCTATTCG TGTCAATGTT 
TTTGGTTCAG CTGGCCAATC TTTTGGTGCT TTCTTAGCTC CAGGTGTTAC ACTCCAATTA 
GAAGGTGACT GTAACGATTA TGTCGGCAAA GGCTTATCTG GCGGTCGCTT GATTATTTAT 
CCTCCTAGGG TTTCTCCCTT TAAGCCCGAA GAAAATATGA TCATTGGTAA TGTCTGTCTG 
TATGGTGCCA CTTCTGGTCA TGCTTTTATT TCTGGTGTGG CAGCAGAGCG TTTCGCTGTT 
CGTAACTCTG GTGCAATTGC TGTAGTTGAA GGCGTTGGTG ACCACGGTTG TGAGTACATG 
ACTGGTGGTC GTGTTGTAAT TCTTGGTTCA ACTGGCAGAA ACTTCGCTGC TGGTATGTCT 
GGTGGTATTG CATATGTCTA CGATATGCAA ATGGACTTTG CTGGTAAAAT TAACACAGAA 
ATGGTCGATA TTTCTTCTGT CACTGATGCT GCTGAAATTG CTTTCCTCAG AGGACTTATT 
CAAGACCATA GGCATTACAC GGGCTCACAG GTGGCTGACC GTATATTAAG TGATTTCCCT 
CGTCACCTTA GCCGCTTTGT TAAGGTTCTT CCAAGAGAGT ACAAGGCTGT ATTGGAACGT 
GAAGCTGCTA AGAAGGAAGA AGCCAAGAGA CTACAGTATC CCAAAGCTTT CATGCCAGGA 
AATCCTATTC GTCAACAAAT TGAGGAAACT AATGCTCAGA TTGCTGATGT GGAAGATACC 
TTAGGTGCTA CTGTGAAAAA GAGCGCTCCT TTGGACAAGC TTCGTGGATT TATGAAATAC 
CAACGCCGCA GTGAACATTA TCGTAACCCT CTTAAGAGAA CGAATGATTG GAAAGAACTT 
TCCGTTCGTC TTCGTGAAGA TGAATTACGT GTTCAAACTG CCAGATGTAT GGACTGTGGT 
ACTCCTTTCT GTCAATCTGA TTATGGTTGT CCTATTTCCA ACAAAATTTT CACTTGGAAT 
GATTTGGTTT TCAAACAACA GTGGAAAGAG GCCTTAACCC AGCTTTTGTT GACCAATAAT 
TTTCCAGAAT TTACAGGCCG TGTCTGCCCT GCTCCTTGTG AAGGCGCTTG TACCTTAGGT 
ATTATTGAAT CGCCTGTTGG AATCAAGAGT GTTGAGCGTG CTATTATTGA CAAGGCTTGG 
GAGGAAGGCT GGATTGTCCC CAGACCTCCT GCTGAACGTA CTGGTCGTCG TGTTGCCATT 
ATCGGTTCTG GGCCTGCAGG TCTTGCTGCC GCTGACCAAC TCAACCGCGC TGGTCACCAT 
GTAGTAATTT ATGAGCGTGC CGATCGTCCA GGTGGTCTTC TTCAATATGG TATTCCTAAT 
ATGAAACTAG ACAAGAAGGT TGTTGAGCGT CGTATCCAGC TCATGATTGA CGAAGGTATT 
GAAGTACTTA CAAACGTTGA AGTCGGTAAG AACGGTGATG TTAGCCTTGA TGAACTTCAC 
AAGGTTTACG ATGCTGTTGT TCTCGCCTCT GGCTCTACGG TTCCTCGTGA TTTGCCCATT 
CCCAACCGTG ATTCTAAGGG TATTCACTTT GCCATGGAGT TCCTTCACAA AAATACTAAG 
AGTCTTCTTG ATAGTGAACT CAAAGATGGC AATTATATTA GTGCTAAGGG CAAGGATGTA 
ATTGTTATTG GAGGAGGTGA TACTGGTAAT GATTGTTTGG GTACCAGTGT TCGTCATGGT 
GCTAAAAGTG TTAGAAATTT GGAGTTACTT CCCATTCCTC CTCGTGAACG TGCCTTCGAC 
AACCCTTGGC CTCAATACCC TCGTGTCTTC CGCGTTGATT ATGGACATGC TGAAGTACAA 
GCTCATTATG GTCAAGATTT CCGTGAATAC TCTATTTTAA CTAAGAGTTT TGAAAAGGAT 
GAAGATGGCA ATGTTAAGGG TATTAATACT GTTCGTATTG AGTGGACTAA GAACAGTAAG 
GGTCGCTGGA TCATGAAGGA GATTCGTAAC TCTGAGGAGT TTTTCCCTGC AGATTTAGTC 
ATCCTTGCTT TAGGTTTCTT AGGTCCTGAG GAGCAAGCTA CTGCTGGAAT GAACGTTGAT 
AGAGATGCTC GTTCTAACAT CAGCACTCCT ACTAAATCGT ATGAAACTAG TGTTCCTGGC 
ATTTACGCTG CTGGTGATTG CCGCCGTGGT CAAAGTTTGG TCGTATGGGG TATTCAAGAA 
GGTCGTCAAT GTGCTCGTGA AATTGATTTG AAATTCCAGG GTAAGACATT TTTGCCTGGT 
GATGGAGGCC TTGTAAAGCG CACTGTTAAT TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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glt1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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