SPBC29A10.10c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPBC29A10.10c
Entrez Gene ID	2540523
Full Name	tRNA-splicing endonuclease positive effector (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021966.2	NP_596055.1	putative tRNA-splicing endonuclease positive effector

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	sen1	NP_594119.1
Schizosaccharomyces pombe (fission yeast)	SPBC29A10.10c	NP_596055.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPBC29A10.10c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPBC29A10.10c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101028	NM_001021966.2 Latest version!	Schizosaccharomyces pombe putative tRNA-splicing endonuclease positive effector (SPBC29A10.10c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPBC29A10.10c ( NM_001021966.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596055.1 Schizosaccharomyces pombe putative tRNA-splicing endonuclease positive effector (SPBC29A10.10c), mRNA.

Price & Availability↑

CloneID	OSc101028
Clone ID Related Accession (Same CDS sequence)	NM_001021966.2
Accession Version	NM_001021966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative tRNA-splicing endonuclease positive effector
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021966.1.

ATGGAAAATG ACGAGGCTTT TGATAGATTA ATAAGAAAAA TTCAGGAAAA TCAAAAACAC 
CCATCTTTCG AAGGGTCCAA TAAAGTTTTG CATTTGGCGC TTTCATACCT AAATACTAAC 
AGACAAAACC CTCATTGGGT TTGTGATCCT AAATTACAAG TAGCAGTTCG TGAATGTCTT 
TTTCTATTTT CTTTTCAAAA TGATAACGAG TATCTGGTAT GGTTCAAAAA ACATTTGAAC 
GAACGCCTTC AGTTATGCCC CAAATGTATA GTTAAATATC ACCAATCTTT AGATGATTTC 
AAAAGTCTAT GTTTAAACAT TTTTCACTTT GACCCAAATA CTCTTGAGAT TGTAATGGAG 
AAAATTTATA GTTGGGACAT TTTTCGGTTA CAGGAAGTTC TCAAAACAGT GCCTAAAATC 
GACACGTCCT CGGCCTCTAA ACCTATACTA TGCGCCTTCT ACGAAATCTT ATACTCTCCT 
CGTATTTTAC ATAATAAAGA AATCTTTCCC TTGTTCCGAA ACAGATTTCT TGAGTCCGGT 
TTTCTTCGGC TCTCGAAAAA CCTAGTGCCA GGTGTCATAA GTCTTCTCTT CTCGAGAGAT 
GATGAGCTCC GAAGATGGGC TTGCAGTATT TTAAGTGATG TAAAAACAAT CTCCGATAAT 
GAATTTAAAA TCCTTGAGGG TCCCCTTCTT CAGGAAATTC GTTCTTTAGA CCAGAAGAAA 
GAAAATGAGA ACGAGATGCA AATTTTTCTG AAAGGTTTAT CGTTGCTGTT AAAGCATGTT 
GATCATCTTT ATTTTAGGGG ACTTACAGGA AAAAATTTCA ATGTCGCCGA TTTTATTCTT 
TCGGGATTGC AAAGCGACCA TACTAATTTA TGTCCGTCTT TCCTTGTATG TTTACATTCT 
TTGTTATACT GCTATGGACG TAATTTTTGG ACGCCAGAAA TTTCTCCCAG TGAAGTAATA 
GATAAAATTT TTAATGGTAA TGCTTATAAG AGGTGGACAG AAGAAAATAC ATCCAATTTT 
TCAAACGACC AAAAGGCAGA GAATCCGTTT GAATGGGCTA CCTCTCTTTT GAAGACAATT 
TCAATCATTG ATGAAGTTGA ATTAATTAAC AAAATTTTGA TTAATTACAC CATGCAATAT 
AAAAAGGAAC TGGAAGTCGG TTTCAAAAAT GTTCAGTTAC TTCAATCACT TGCTGACATA 
TTTTCAGTTT TGTTTTCCGA ATATTTTATG ATTTTCCCCC ATAGTGATCA AAGTTTACAA 
AACAAAGTAT TTGAATTACA TTCCACTTTA GCGATTAAGT TTGACGAGCT GTTTCGTTTG 
CTAAACTTGG AAAATGAAGA GTCTGTTGAA TGGAGGCTTA TTAAGAAAGT TTTTGAATAC 
CGATTGAATT TAGATCTTTA TATTTTGAAG CAATTCTATT GCCATTATTT GGACAGAAAT 
AGAAAGCCAC CCTTGAATAT CAAATCGGTA AGTGAGTCTT TTAAAAATTT TTGGGTATAT 
TTGCAAAACG TATTTCGAGA GGAGAAGTTC TTTATGGTTC GTATTGTTTT TCGTGCTTGC 
TCTACAGTTT GCTTGATTGA TAAGTTGCCG CCTAAGAAAG TTGACATTCC GTTCAAAAGT 
GATTTCGAAA ATGCACTAAA CGGATTTGTT GCAACATTTT CTGAAATGCT TATTCAGACT 
CAATGTTGGC ATTTGTCGGC ACAAACCCAA TTGGTTTCAA ATCCTTTAAC TTTTCGTGGA 
ATCTTTTCAC TCGTTTTTTC TCCAATTCTT GAGATCTCAA CAGGAAGTAT CTCAATTATT 
AAATCTATTT CGCTTTCTGA CAGCGTTGTT GATACTATAG GAGAGCTATT GAGAAGCCAG 
TTTTCAAATA CACTAAATTC TTCATGTTAT GTTCTTCTGC AGTGGCTTAA AGTCAGAAAC 
TTTGGAGGAG CCAAGCACAT TGTTTATTTT AATAAATTAA TTATAAACAC TATTTTTGAC 
TCTGTTGATG GCTTAACTTC TAAGGATGCG ACTTTTGCAA AACAAGTGGA AAAGAAAGAA 
TCCCTTAAGA ATTTCTGGGA ATCGTTATGG AAATTCTTTA CTCATTTTTT CATTGTTCTT 
CCTACTTGGC CAGTTCATGA TAAGGACAAT TTAGTTGATT TAATGCGGGA TACCCTTGAT 
TTTTGCGATA TGCTCATTAA TATTTTTGAG GAAGTTAACG GATTCATTTT TGGATTATCG 
GATAACGATA TTAAAATTGT TTCGGCTAAT GATAAGGGCA GTGCTTTAGC TATGTGTATA 
GCTGATTCTT TGGTTACTGT ATCTTATTGG CTTAAGTTAA CTGATTCTTC ACTTCTAACA 
TCCGTTGTGA AAGTGATTTG TAAAATGCTT AAAATTTGTA AAAAGTTGGA GTGTCCAATT 
AGTCAAAATG TGATTGATAT TATCCATAGG GCATCAATTA CCAGTGATGA ACAAACAATT 
CTTACATTCA CTGAGAGAGA GGATCTTTTT ATATCGCTCA CACCATACCT TTCAGAAGAC 
GTTCTTAATC ACTCTCCTTT CAATGATACC AATACGCTTG AAACTAAGTT ACAGAGTGAT 
GATCGGGGTT TGCTTTCTAA AGACCAGACT ATTGGGATTG CGAAAAAATT ACCTGAGTCA 
AACATTAGTA CTTCAAATCA TTTTTTGCTT CCTCCGAAGG CTATTTCTGC TTCTAAAGCC 
ATCAATAGAA ATGCTCAAAA ATCTCAAAAT TTAAATTTTT TGAAATCTAA ACAAGAGACA 
ACACAGCGAA TTCGCGAATC CGCGAAAGTT CCAAGAACCA GTGCGGGGAA CCATCTTTCT 
GAGAAACTTA ATTCGGATAA TCATATACCA AAAGCTTTGC AAAAGCTCGA TTCTGCTGAC 
CCAATTCGCA AGCCTTCACT TTTGCACACT TCTAAAAGTT ATTCTAATCC AGATGACAAA 
AATACTTCAA CTTCCGATGA AGATACCTCG GAGTCCGAAG AAGAGTCTAG TAACGGTCTA 
TTCTCGCTTG CACGTGAAGC TAATTCTCAT GCCTCTAAAT CTCTTCCACA AAGAAGGCAG 
ATTCAGTTCT TGGACTTTGA TTCCCTTAAA ACAAAAAATG TAGTTCATCC TACTCAATTG 
CGCCGTAATA CTCAACAAAG TGCACAACTT GCTCGACTCC GGCTAAACCC TGATGTTCAA 
GAGTTTTACA AAGTAATTTT GGGTTGGAAC CCATTAGCTG ATTCTTTTTC GGCGTCTAAT 
GTTGAGATGC AGTGCGTCCA AGCTAAATTC ACGTATAATG ATTCAAACGC TTATGAGAAA 
GTTTTCAAAC CAATGTTGTT TCATGAATGT TGGGCTCAAG TGAAAAGTGC CGTTGAAGAA 
AAGCAATACC CTCCCATAGA TTTAATCCTC AACACCCGCA GTACAGTTGA TAACTTTGTC 
GATATTTACT TTACGTCCTG TTCACCTACC GAAGTCTCTT TTTTAAGTGA TACAGATATT 
TGCTTACTGT CAAAATCACA AAGTTCGGGC GATACTAATA ACCCAAAATC TTTTCAGTTG 
TGCAAAATTC AATCCATTTC TCGTAAAAAA GAATCACTTG AACTCTGTCT GCGGATGAAT 
ATTGAATCAA TTGATCTACA GGAGTATGCA CCGAACATTA GGTTCACAGC ACAAAAATTA 
TTTAATGCTA CTACTAGCTT ACGTGAATTT GCTGCTTTAA AATCTCTTCG CCATTTACCA 
TTGTCTCAGA GAATTCTGGA TGCCAATGTA ACGCGTCTAC CTTCAAATTT TACAGATGAC 
AAAAAGCAAA AAATAATGAA GTCTTATGGT GTAAATGAAC CCCAGGCATA TGCGATTTAT 
GCATCTTCTG TAAATGATGG TTTTACGCTC ATTCAAGGGC CTCCTGGAAC TGGAAAAACT 
AAAACCATTC TAGGCATGAT TGGAGCTGTA CTTACTTCTT CTAGTCAAGG GTTGCAATTT 
AATGTCCCAG GACAGACGCG AAAAACCTCA AAGAATAAGA TTTTAATATG CGCTCCTAGT 
AATGCAGCCA TAGATGAAAT TCTCCTTCGT ATCAAAGCCG GTGTTTACGA TCATGAGGGT 
ATCAAATTTT TTCCAAAAGT TATTCGTGTT GGATTTGGTG ACTCCATAAG CGTTCACGCT 
AAAGAATTTA CTTTGGAAGA GCAAATGATA AAGCAAATGG AACTTACGAA CCTAAAGAAA 
GATCAAGAAG CAAACAATTC CAGTGATACT CGGAAAAAAT ATGACTCAAT TATTAAAAAA 
AGGGACTCTT TACGCGAAGA TCTAGAAAAG TTTCGTAGTA CTGGAAAAAA TAGTTCAATA 
TTGGAAGCAC AGCTTCGTGA GATTACCAAA CAGAAAAACA TGCTTGAACA GAGCCTTGAT 
GATATGAGAG AACGTCAACG CTCAACTAAT CGTAATCTGG ATGTTTTGAA AAAACAAATA 
CAAAATCAAC TACTTCAGGA AGCTGATATT GTATGTGCTA CCTTATCTGC GAGTGGTCAT 
GAACTACTTT TAAATGCGGG TTTGACTTTT CGAACTGTCA TAATTGATGA AGCTGCACAA 
GCCGTTGAAT TAAGTTCAAT CATACCTCTT AAGTATGGCT GTGAGAGCTG TGTAATGGTT 
GGTGATCCGA ATCAGCTTCC TCCTACTGTG CTATCAAAAA CCTCTGCAAA GTTTGGATAT 
TCACAATCTC TATATGTCAG AATGTTCAAA CAGCATAATG AGAGCGCTTG TCTTCTTAGC 
ATACAGTATA GAATGAATCC TGAAATTAGC CGATTTCCCA GCAAGTTTTT CTATAATTCA 
AAACTACTTG ACGGTCCCAA TATGTCAGCA GTGACATCTC GACCTTGGCA CGAGGATCCG 
CAGCTAGGTA TTTATCGATT TTTTAATGTT CACGGGACGG AAGCTTTTTC CAACTCAAAG 
TCTCTGTACA ATGTGGAAGA GGCTTCGTTT ATTTTATTAT TATATGAGAG GCTAATTCAA 
TGTTATCTAA ACATTGATTT TGAGGGGAAA ATTGGAGTTG TTACCCCTTA TAGAAGTCAA 
GTACAACAGC TTCGATCACA ATTTCAAAGA AAGTATGGTT CCATTATATT CAAGCATCTT 
GATATTCATA CCGTCGATGG GTTTCAGGGA CAGGAAAAGG ACATTATAAT TTTTTCTTGC 
GTTAGGAGTT CGATGAGCGG TGGAATCGGT TTCCTTCAAG ATCTTCGTCG GCTTAATGTA 
GCTTTAACAA GAGCTAAATC ATCTTTGTAT ATCGTAGGCA ATTCTAAACC GCTAATGCAA 
GAGGATATTT TTTATAGCTT GATTGAAGAC GCAAAAACTC GTGGTGTTTG GAGAGACTTG 
AGTGCTAATC AATTTAAGAA CTCAAAAAGT ATATCAAACG TTTCGACACA CCTCGCATCT 
AACAATTTAA ATTTAGCCTC ACGGGATACC CCAATTAAGT CGCCGTCGGT TGGTATCTGT 
GAGGAAAAAC AAGAAGCACA TAAAGTCAAA AAACGGCACA ATATAGATAG TGCTAATTTG 
TCAAGAGGAA CTGAGAGAGA TGAAGATATA CCGAATAAGC GCGCTAAGAA CAAAGTTTCC 
ACTGATCAGA CTGCTGCTGA TAATAAGGTC ACGAAACCAC GCCTTGATGA ATCATCCAGT 
TCCAAGCAAG ATGTGTTAAA CAAAATAGAC GAATCTGAAA TTGAACAAGC GTCTTCCAAA 
AAGCCGGGTT ATGTGGAAAA AAATAAAGAT AAAGGCCATA TGAAAAAATC AAAAAAGCCC 
AAATCGAAAC TTGCTCTGGC GGCGATGGCC CACGGTTTTG CACCTCCAAA AGTTGAGCAT 
TTCAAAAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596055.1
CDS	84..5918
Translation	MENDEAFDRLIRKIQENQKHPSFEGSNKVLHLALSYLNTNRQNPHWVCDPKLQVAVRECLFLFSFQNDNEYLVWFKKHLNERLQLCPKCIVKYHQSLDDFKSLCLNIFHFDPNTLEIVMEKIYSWDIFRLQEVLKTVPKIDTSSASKPILCAFYEILYSPRILHNKEIFPLFRNRFLESGFLRLSKNLVPGVISLLFSRDDELRRWACSILSDVKTISDNEFKILEGPLLQEIRSLDQKKENENEMQIFLKGLSLLLKHVDHLYFRGLTGKNFNVADFILSGLQSDHTNLCPSFLVCLHSLLYCYGRNFWTPEISPSEVIDKIFNGNAYKRWTEENTSNFSNDQKAENPFEWATSLLKTISIIDEVELINKILINYTMQYKKELEVGFKNVQLLQSLADIFSVLFSEYFMIFPHSDQSLQNKVFELHSTLAIKFDELFRLLNLENEESVEWRLIKKVFEYRLNLDLYILKQFYCHYLDRNRKPPLNIKSVSESFKNFWVYLQNVFREEKFFMVRIVFRACSTVCLIDKLPPKKVDIPFKSDFENALNGFVATFSEMLIQTQCWHLSAQTQLVSNPLTFRGIFSLVFSPILEISTGSISIIKSISLSDSVVDTIGELLRSQFSNTLNSSCYVLLQWLKVRNFGGAKHIVYFNKLIINTIFDSVDGLTSKDATFAKQVEKKESLKNFWESLWKFFTHFFIVLPTWPVHDKDNLVDLMRDTLDFCDMLINIFEEVNGFIFGLSDNDIKIVSANDKGSALAMCIADSLVTVSYWLKLTDSSLLTSVVKVICKMLKICKKLECPISQNVIDIIHRASITSDEQTILTFTEREDLFISLTPYLSEDVLNHSPFNDTNTLETKLQSDDRGLLSKDQTIGIAKKLPESNISTSNHFLLPPKAISASKAINRNAQKSQNLNFLKSKQETTQRIRESAKVPRTSAGNHLSEKLNSDNHIPKALQKLDSADPIRKPSLLHTSKSYSNPDDKNTSTSDEDTSESEEESSNGLFSLAREANSHASKSLPQRRQIQFLDFDSLKTKNVVHPTQLRRNTQQSAQLARLRLNPDVQEFYKVILGWNPLADSFSASNVEMQCVQAKFTYNDSNAYEKVFKPMLFHECWAQVKSAVEEKQYPPIDLILNTRSTVDNFVDIYFTSCSPTEVSFLSDTDICLLSKSQSSGDTNNPKSFQLCKIQSISRKKESLELCLRMNIESIDLQEYAPNIRFTAQKLFNATTSLREFAALKSLRHLPLSQRILDANVTRLPSNFTDDKKQKIMKSYGVNEPQAYAIYASSVNDGFTLIQGPPGTGKTKTILGMIGAVLTSSSQGLQFNVPGQTRKTSKNKILICAPSNAAIDEILLRIKAGVYDHEGIKFFPKVIRVGFGDSISVHAKEFTLEEQMIKQMELTNLKKDQEANNSSDTRKKYDSIIKKRDSLREDLEKFRSTGKNSSILEAQLREITKQKNMLEQSLDDMRERQRSTNRNLDVLKKQIQNQLLQEADIVCATLSASGHELLLNAGLTFRTVIIDEAAQAVELSSIIPLKYGCESCVMVGDPNQLPPTVLSKTSAKFGYSQSLYVRMFKQHNESACLLSIQYRMNPEISRFPSKFFYNSKLLDGPNMSAVTSRPWHEDPQLGIYRFFNVHGTEAFSNSKSLYNVEEASFILLLYERLIQCYLNIDFEGKIGVVTPYRSQVQQLRSQFQRKYGSIIFKHLDIHTVDGFQGQEKDIIIFSCVRSSMSGGIGFLQDLRRLNVALTRAKSSLYIVGNSKPLMQEDIFYSLIEDAKTRGVWRDLSANQFKNSKSISNVSTHLASNNLNLASRDTPIKSPSVGICEEKQEAHKVKKRHNIDSANLSRGTERDEDIPNKRAKNKVSTDQTAADNKVTKPRLDESSSSKQDVLNKIDESEIEQASSKKPGYVEKNKDKGHMKKSKKPKSKLALAAMAHGFAPPKVEHFKRK

Target ORF information:

RefSeq Version	NM_001021966.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative tRNA-splicing endonuclease positive effector (SPBC29A10.10c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021966.2

ORF Insert Sequence:

ATGGAAAATG ACGAGGCTTT TGATAGATTA ATAAGAAAAA TTCAGGAAAA TCAAAAACAC 
CCATCTTTCG AAGGGTCCAA TAAAGTTTTG CATTTGGCGC TTTCATACCT AAATACTAAC 
AGACAAAACC CTCATTGGGT TTGTGATCCT AAATTACAAG TAGCAGTTCG TGAATGTCTT 
TTTCTATTTT CTTTTCAAAA TGATAACGAG TATCTGGTAT GGTTCAAAAA ACATTTGAAC 
GAACGCCTTC AGTTATGCCC CAAATGTATA GTTAAATATC ACCAATCTTT AGATGATTTC 
AAAAGTCTAT GTTTAAACAT TTTTCACTTT GACCCAAATA CTCTTGAGAT TGTAATGGAG 
AAAATTTATA GTTGGGACAT TTTTCGGTTA CAGGAAGTTC TCAAAACAGT GCCTAAAATC 
GACACGTCCT CGGCCTCTAA ACCTATACTA TGCGCCTTCT ACGAAATCTT ATACTCTCCT 
CGTATTTTAC ATAATAAAGA AATCTTTCCC TTGTTCCGAA ACAGATTTCT TGAGTCCGGT 
TTTCTTCGGC TCTCGAAAAA CCTAGTGCCA GGTGTCATAA GTCTTCTCTT CTCGAGAGAT 
GATGAGCTCC GAAGATGGGC TTGCAGTATT TTAAGTGATG TAAAAACAAT CTCCGATAAT 
GAATTTAAAA TCCTTGAGGG TCCCCTTCTT CAGGAAATTC GTTCTTTAGA CCAGAAGAAA 
GAAAATGAGA ACGAGATGCA AATTTTTCTG AAAGGTTTAT CGTTGCTGTT AAAGCATGTT 
GATCATCTTT ATTTTAGGGG ACTTACAGGA AAAAATTTCA ATGTCGCCGA TTTTATTCTT 
TCGGGATTGC AAAGCGACCA TACTAATTTA TGTCCGTCTT TCCTTGTATG TTTACATTCT 
TTGTTATACT GCTATGGACG TAATTTTTGG ACGCCAGAAA TTTCTCCCAG TGAAGTAATA 
GATAAAATTT TTAATGGTAA TGCTTATAAG AGGTGGACAG AAGAAAATAC ATCCAATTTT 
TCAAACGACC AAAAGGCAGA GAATCCGTTT GAATGGGCTA CCTCTCTTTT GAAGACAATT 
TCAATCATTG ATGAAGTTGA ATTAATTAAC AAAATTTTGA TTAATTACAC CATGCAATAT 
AAAAAGGAAC TGGAAGTCGG TTTCAAAAAT GTTCAGTTAC TTCAATCACT TGCTGACATA 
TTTTCAGTTT TGTTTTCCGA ATATTTTATG ATTTTCCCCC ATAGTGATCA AAGTTTACAA 
AACAAAGTAT TTGAATTACA TTCCACTTTA GCGATTAAGT TTGACGAGCT GTTTCGTTTG 
CTAAACTTGG AAAATGAAGA GTCTGTTGAA TGGAGGCTTA TTAAGAAAGT TTTTGAATAC 
CGATTGAATT TAGATCTTTA TATTTTGAAG CAATTCTATT GCCATTATTT GGACAGAAAT 
AGAAAGCCAC CCTTGAATAT CAAATCGGTA AGTGAGTCTT TTAAAAATTT TTGGGTATAT 
TTGCAAAACG TATTTCGAGA GGAGAAGTTC TTTATGGTTC GTATTGTTTT TCGTGCTTGC 
TCTACAGTTT GCTTGATTGA TAAGTTGCCG CCTAAGAAAG TTGACATTCC GTTCAAAAGT 
GATTTCGAAA ATGCACTAAA CGGATTTGTT GCAACATTTT CTGAAATGCT TATTCAGACT 
CAATGTTGGC ATTTGTCGGC ACAAACCCAA TTGGTTTCAA ATCCTTTAAC TTTTCGTGGA 
ATCTTTTCAC TCGTTTTTTC TCCAATTCTT GAGATCTCAA CAGGAAGTAT CTCAATTATT 
AAATCTATTT CGCTTTCTGA CAGCGTTGTT GATACTATAG GAGAGCTATT GAGAAGCCAG 
TTTTCAAATA CACTAAATTC TTCATGTTAT GTTCTTCTGC AGTGGCTTAA AGTCAGAAAC 
TTTGGAGGAG CCAAGCACAT TGTTTATTTT AATAAATTAA TTATAAACAC TATTTTTGAC 
TCTGTTGATG GCTTAACTTC TAAGGATGCG ACTTTTGCAA AACAAGTGGA AAAGAAAGAA 
TCCCTTAAGA ATTTCTGGGA ATCGTTATGG AAATTCTTTA CTCATTTTTT CATTGTTCTT 
CCTACTTGGC CAGTTCATGA TAAGGACAAT TTAGTTGATT TAATGCGGGA TACCCTTGAT 
TTTTGCGATA TGCTCATTAA TATTTTTGAG GAAGTTAACG GATTCATTTT TGGATTATCG 
GATAACGATA TTAAAATTGT TTCGGCTAAT GATAAGGGCA GTGCTTTAGC TATGTGTATA 
GCTGATTCTT TGGTTACTGT ATCTTATTGG CTTAAGTTAA CTGATTCTTC ACTTCTAACA 
TCCGTTGTGA AAGTGATTTG TAAAATGCTT AAAATTTGTA AAAAGTTGGA GTGTCCAATT 
AGTCAAAATG TGATTGATAT TATCCATAGG GCATCAATTA CCAGTGATGA ACAAACAATT 
CTTACATTCA CTGAGAGAGA GGATCTTTTT ATATCGCTCA CACCATACCT TTCAGAAGAC 
GTTCTTAATC ACTCTCCTTT CAATGATACC AATACGCTTG AAACTAAGTT ACAGAGTGAT 
GATCGGGGTT TGCTTTCTAA AGACCAGACT ATTGGGATTG CGAAAAAATT ACCTGAGTCA 
AACATTAGTA CTTCAAATCA TTTTTTGCTT CCTCCGAAGG CTATTTCTGC TTCTAAAGCC 
ATCAATAGAA ATGCTCAAAA ATCTCAAAAT TTAAATTTTT TGAAATCTAA ACAAGAGACA 
ACACAGCGAA TTCGCGAATC CGCGAAAGTT CCAAGAACCA GTGCGGGGAA CCATCTTTCT 
GAGAAACTTA ATTCGGATAA TCATATACCA AAAGCTTTGC AAAAGCTCGA TTCTGCTGAC 
CCAATTCGCA AGCCTTCACT TTTGCACACT TCTAAAAGTT ATTCTAATCC AGATGACAAA 
AATACTTCAA CTTCCGATGA AGATACCTCG GAGTCCGAAG AAGAGTCTAG TAACGGTCTA 
TTCTCGCTTG CACGTGAAGC TAATTCTCAT GCCTCTAAAT CTCTTCCACA AAGAAGGCAG 
ATTCAGTTCT TGGACTTTGA TTCCCTTAAA ACAAAAAATG TAGTTCATCC TACTCAATTG 
CGCCGTAATA CTCAACAAAG TGCACAACTT GCTCGACTCC GGCTAAACCC TGATGTTCAA 
GAGTTTTACA AAGTAATTTT GGGTTGGAAC CCATTAGCTG ATTCTTTTTC GGCGTCTAAT 
GTTGAGATGC AGTGCGTCCA AGCTAAATTC ACGTATAATG ATTCAAACGC TTATGAGAAA 
GTTTTCAAAC CAATGTTGTT TCATGAATGT TGGGCTCAAG TGAAAAGTGC CGTTGAAGAA 
AAGCAATACC CTCCCATAGA TTTAATCCTC AACACCCGCA GTACAGTTGA TAACTTTGTC 
GATATTTACT TTACGTCCTG TTCACCTACC GAAGTCTCTT TTTTAAGTGA TACAGATATT 
TGCTTACTGT CAAAATCACA AAGTTCGGGC GATACTAATA ACCCAAAATC TTTTCAGTTG 
TGCAAAATTC AATCCATTTC TCGTAAAAAA GAATCACTTG AACTCTGTCT GCGGATGAAT 
ATTGAATCAA TTGATCTACA GGAGTATGCA CCGAACATTA GGTTCACAGC ACAAAAATTA 
TTTAATGCTA CTACTAGCTT ACGTGAATTT GCTGCTTTAA AATCTCTTCG CCATTTACCA 
TTGTCTCAGA GAATTCTGGA TGCCAATGTA ACGCGTCTAC CTTCAAATTT TACAGATGAC 
AAAAAGCAAA AAATAATGAA GTCTTATGGT GTAAATGAAC CCCAGGCATA TGCGATTTAT 
GCATCTTCTG TAAATGATGG TTTTACGCTC ATTCAAGGGC CTCCTGGAAC TGGAAAAACT 
AAAACCATTC TAGGCATGAT TGGAGCTGTA CTTACTTCTT CTAGTCAAGG GTTGCAATTT 
AATGTCCCAG GACAGACGCG AAAAACCTCA AAGAATAAGA TTTTAATATG CGCTCCTAGT 
AATGCAGCCA TAGATGAAAT TCTCCTTCGT ATCAAAGCCG GTGTTTACGA TCATGAGGGT 
ATCAAATTTT TTCCAAAAGT TATTCGTGTT GGATTTGGTG ACTCCATAAG CGTTCACGCT 
AAAGAATTTA CTTTGGAAGA GCAAATGATA AAGCAAATGG AACTTACGAA CCTAAAGAAA 
GATCAAGAAG CAAACAATTC CAGTGATACT CGGAAAAAAT ATGACTCAAT TATTAAAAAA 
AGGGACTCTT TACGCGAAGA TCTAGAAAAG TTTCGTAGTA CTGGAAAAAA TAGTTCAATA 
TTGGAAGCAC AGCTTCGTGA GATTACCAAA CAGAAAAACA TGCTTGAACA GAGCCTTGAT 
GATATGAGAG AACGTCAACG CTCAACTAAT CGTAATCTGG ATGTTTTGAA AAAACAAATA 
CAAAATCAAC TACTTCAGGA AGCTGATATT GTATGTGCTA CCTTATCTGC GAGTGGTCAT 
GAACTACTTT TAAATGCGGG TTTGACTTTT CGAACTGTCA TAATTGATGA AGCTGCACAA 
GCCGTTGAAT TAAGTTCAAT CATACCTCTT AAGTATGGCT GTGAGAGCTG TGTAATGGTT 
GGTGATCCGA ATCAGCTTCC TCCTACTGTG CTATCAAAAA CCTCTGCAAA GTTTGGATAT 
TCACAATCTC TATATGTCAG AATGTTCAAA CAGCATAATG AGAGCGCTTG TCTTCTTAGC 
ATACAGTATA GAATGAATCC TGAAATTAGC CGATTTCCCA GCAAGTTTTT CTATAATTCA 
AAACTACTTG ACGGTCCCAA TATGTCAGCA GTGACATCTC GACCTTGGCA CGAGGATCCG 
CAGCTAGGTA TTTATCGATT TTTTAATGTT CACGGGACGG AAGCTTTTTC CAACTCAAAG 
TCTCTGTACA ATGTGGAAGA GGCTTCGTTT ATTTTATTAT TATATGAGAG GCTAATTCAA 
TGTTATCTAA ACATTGATTT TGAGGGGAAA ATTGGAGTTG TTACCCCTTA TAGAAGTCAA 
GTACAACAGC TTCGATCACA ATTTCAAAGA AAGTATGGTT CCATTATATT CAAGCATCTT 
GATATTCATA CCGTCGATGG GTTTCAGGGA CAGGAAAAGG ACATTATAAT TTTTTCTTGC 
GTTAGGAGTT CGATGAGCGG TGGAATCGGT TTCCTTCAAG ATCTTCGTCG GCTTAATGTA 
GCTTTAACAA GAGCTAAATC ATCTTTGTAT ATCGTAGGCA ATTCTAAACC GCTAATGCAA 
GAGGATATTT TTTATAGCTT GATTGAAGAC GCAAAAACTC GTGGTGTTTG GAGAGACTTG 
AGTGCTAATC AATTTAAGAA CTCAAAAAGT ATATCAAACG TTTCGACACA CCTCGCATCT 
AACAATTTAA ATTTAGCCTC ACGGGATACC CCAATTAAGT CGCCGTCGGT TGGTATCTGT 
GAGGAAAAAC AAGAAGCACA TAAAGTCAAA AAACGGCACA ATATAGATAG TGCTAATTTG 
TCAAGAGGAA CTGAGAGAGA TGAAGATATA CCGAATAAGC GCGCTAAGAA CAAAGTTTCC 
ACTGATCAGA CTGCTGCTGA TAATAAGGTC ACGAAACCAC GCCTTGATGA ATCATCCAGT 
TCCAAGCAAG ATGTGTTAAA CAAAATAGAC GAATCTGAAA TTGAACAAGC GTCTTCCAAA 
AAGCCGGGTT ATGTGGAAAA AAATAAAGAT AAAGGCCATA TGAAAAAATC AAAAAAGCCC 
AAATCGAAAC TTGCTCTGGC GGCGATGGCC CACGGTTTTG CACCTCCAAA AGTTGAGCAT 
TTCAAAAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SPBC29A10.10c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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