sen1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	sen1
Entrez Gene ID	2541556
Full Name	ATP-dependent 5' to 3' DNA/RNA helicase Sen1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019543.2	NP_594119.1	helicase Sen1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	sen1	NP_594119.1
Schizosaccharomyces pombe (fission yeast)	SPBC29A10.10c	NP_596055.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sen1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sen1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102159	NM_001019543.2 Latest version!	Schizosaccharomyces pombe helicase Sen1 (sen1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sen1 ( NM_001019543.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594119.1 Schizosaccharomyces pombe helicase Sen1 (sen1), mRNA.

Price & Availability↑

CloneID	OSc102159
Clone ID Related Accession (Same CDS sequence)	NM_001019543.2
Accession Version	NM_001019543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	helicase Sen1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019543.1.

ATGGCGGAGA ATCTTAGCGA TCAAGATTGT TTTGAAAAAT TATCGTCTAG TAAAGAGGGT 
CAGCATTGGT TTTGCTCAGG ACTACTGACT CAGTATATAC AACCTACCTT CTTTTGGTTT 
GCACATGATG AAACTCCGAG CTTTAAATGG GTATTAAATG CCTATCATGA GCGTTTACGC 
TCTTGTACTT CTTGTATACA AGCTTATTAT GAACTTCGAA ATGAATCCCT TGCGAAAGGT 
TCATATTCTT TTACTGGGTT TTCAATTACC GATTTACAGG AAAAGTGGAA TAAATGGGAT 
ATTATCCGTG TCTTAGAGGA CTTCAAGGCC CTGGAGGATG AAACAATTGA CTTCACGTCT 
TTGCCTTGCT TAATTTTCGA AACCCTCTTG AATCCTAAAT TGTTTACCTG TAAAAATATT 
TATAAAAATG CAATAGATGC ATTTAATGGT CTACTTTCAG ATTGGTGTTC TAATATATTT 
CTTCCTGGTT ATTTACTATT TTTCTATGAA GCTTCCCATC CCGATGTTTT GGAATGGGTT 
CATAGCTTTT TTAAGGAAAA TACTGAAATA AGAATTTCTT CTGCTACAGT TGACGCCGTT 
TTTAATACTG TAGTGTTTGA AAGTCAAAAT TCCTCTGACA GTGATTGTAA TTTCGTGTCT 
ATAAGTAGTC CTGAATTTTG GGAACGTACT TACATGTTTT TAGAACTTCT TCCTTTACAA 
TCCATTACTG CTGCATGTGA GTCCATTTTA AAAGATTATC TTTTAAATTT GGTAAAGACT 
TCTGAATCTC TATCCTCTCA ACAAATGTCC TGTCTTCGAA TTTGCTGCAA TTCCTCATCC 
TTTTGGTCTG CCGCTGATTC ATTTAAGGAA GTGTCTTCTT TCTTAAAAAA CTTGCTAAAA 
AATGTTACAG CTACACCTTT TGAAGTATCT GACATGAACT GGGCTTACAT TATTGCTTCA 
TTTTTTCGCA CATGTTTAAA TGACTTTGTT AGTGTTTTTC CGGAATGGCT TAAAGGATTT 
GTAGAAGAGA AGCGTACTAT AGGTTTCATC ATTTATTCGG TTTTGGATGC GTTATTTGAT 
CTATTATCGC TAGATTACAG CTCTCCTTCA TTTCTTAACA ATACACTATC GTTGATGAAT 
GCTAATAGCA TCACTATTTT ACAAGATTAT CCGGAGTACA TGCTGAAACT CAGATTGCAC 
TCTTTACTTT ATGACATTGC ATTTATAAGT TGGTCTCATA AGGCAATCAC TAAAAATCCC 
TCATTTGTTT TCGATCCTTC ATATGAACTC TCTCCTTTTT GGAAGCTTGA CAATCTACAG 
GAAGAAAAAA TATCTGACGT CCTTTTCTCT AAAATTTCAA GTTGTTGCTT TGCCCACGAT 
ATTGAAATAT CCGAAAATTC TACACCGTCT GGAACCATGG TGCAGTTTGC TGAACTATGG 
CAAGTAATGT CGGAATATAT CAGTGGATTT TTAAAAGGAT TTTCTGAAAA GTCGTCTACT 
GAAATTTCAA ATATGCTGGG AGATTCTTCA AAATTTGACA CAGTTGTATC ATTTCTGTTA 
TCTCCTACTC AGCCGCTCTA TGTTAGTGCT TTTCATATTG TCCAAATAAT TACCAATTGC 
ACTAAAAACA GGAATGAGGC TCTTAAAAAA CTGGTTGCAA TGGATTTTCG AGGAATTGTG 
CATGGTCTAG CTGATGCTGT TCTTAACTGG CAATCTATTT TAAGCTTCTT TCCTGCTCTT 
CGAATTATGA GATTTTTGAG CATTACTAAT AAATCCTTAT CATCTGACAA TTCTGCTTTC 
ACAGAAAATG ACATACCTAC CTTGGGTGCT TATTGGCAGT GCATCTGGAA CATATTGGAT 
TTAGTTTTTT CCAACGTTGC CAGATGGTCG CTTAACAATC CTGCTGATAC GGTTAAAGCA 
TTGATGAAAC TAACACTTAA GTTTGTTGAT GATCTTTTCC AAAATGACGG AATCTTCATA 
AAGCTCCTTG CTAAGTTTGA TAGCTTGATT CTGTTAGGCG AAACTTCTGA ATCATTGTTC 
TCTTTTATTA TGTGGCTTAA AATAAACGAT TTAGAATTAA GGGGAATTGT CATCAATAGT 
TTATGCAAGC TTTTCACTAA ATTTTCGAAT TTTGACTACC TTTTCGAGGA TCGCACCGTT 
ACCTTCCTTA CAGATTTTAT CATTAGAAAG CAAAAAGCAC ATTTGTCTGC CGATCAATGT 
AAACAGCTTG CTAATGTTTT GACTCAGGCT AGCCCTGAAG CAAAGACTGT ACTTGAGCAA 
CACCGACTTT CGGAAATGCG AAAAACAAAG AAACAAACAG AACTAACTAA TAGTGCACAT 
GTCATAAAGC CAAGTCCTAC ACCTCAAATT ACTGTGAAAC AGAATACTAC TAAATCTTCA 
AGTGCCCCTC GCATGGGAAT GCTGGAGCAA CTAAAGCAGG AGTATTTGAC TAAACGAAAC 
TTTGAATCTA AACTCAAATC ATCTGCTGTA TCTTCAAGAA AGCCAACATT TAATGAAGTC 
AAGCCAGCTA ACCTTTTAGC TGAGGACCTC TCAGATAATG AGGATGATAT TGATAGAAAG 
CAAGGATTAT TCTCTCTTGC CAAAGCAAAC AAAATTCCAG AGATACGCCA ACAAGAGCGA 
CGACAAGTGC AGCTATTATC TAATTCCACT ATTAAGATGC ATCCTTCACA AATTCGGATG 
ATGACTAATA GGAACGTTGC AAATGTTAAA GCACGCTTAT TTCCAAGTAT GACTGACTTT 
TACAAAGAAA TTTTGAGCTG GGAACCAGCT AATCAGTCAC CTAATCCTGT ACTGAAATTT 
CATAAGCTTG ATGGGAAAAT TATTGATAGT TTTAAAACGG TTGAACATTA TATGGAGGTT 
TTGCAGCCGA TGATATTTAT GGAATGCTGG AGTCAAATTC AGTCAACAAA ATTGGATTTA 
AAATTCTCTC CTGTTGAAGG AATCATGGTT GAAAGAACGG CCGTCAATAA TTTTGTTGAT 
ATAGGAGTCT CAGTGGCTCC CAAGGATCTA TACGGTTATC CCTTATATGA TACGGAAGTA 
GTTTCGTTGG CTTTTAACAA GGAGGATGCT TCATCAATGA AAGGGCTGTG TTGCTTTGCG 
AAAGTTGAAC GCATAGTTAG GCAAACGAAT GGAGTTTTAG TTGTCTTACG TACGTTACCA 
TCTATGGAAA TTTTGAACAA ATTACAAGGT AATTGTGCTT TATGGTTTCT AAAATTAACA 
AATTTGGCTA CATTCACTCG CCAATATGCC GGCATAAGAG GTTTACCATA CTTTCATCTA 
GCAGACGATA TTATTCGTGC GAGGCCTTGT TCTCAACCAG TTAAGCATTC TTCAAGCGAG 
ATAAAAGCAG CTATGAAGCG ATATCAGGTT AACGAACCTC AAGCTAAGGC CATTATGTGC 
GCTCTTGATA ATAACGGATT TACTTTGATT CAAGGTCCTC CCGGTACTGG TAAAACAAAA 
ACAATTATTG GGATTATTTC GGCTTTGCTC GTGGACCTTA GTCGATACCA CATAACACGA 
CCGAATCAAC AATCGAAGAG CACAGAATCA AAGCAACAAA TTTTACTTTG TGCACCTAGT 
AATGCAGCGG TTGACGAAGT TTTATTGCGT CTAAAACGTG GTTTCCTTTT AGAAAATGGT 
GAAAAATATA TACCAAGAGT TGTCCGTATA GGTAATCCGG AAACCATTAA TGTTTCAGTT 
CGCGATTTGT CTTTGGAATA TCAAACTGAA AAACAACTTT TAGAAGTCAA CCAAGGTGCT 
ATTGACCTTG GGTCGCTCCA GGAACTAACT CGCTGGCGTG ATACGTTTTA CGATTGTATT 
CAAAAAATCG AAGAGCTTGA AAAACAAATC GACGTTGCCC GTGATGTTGC TGAAGATACT 
AAATCTTTGG GAAAAGAACT CCAAAATAAA ATAAATGAAA AAAATTTGGC AGAACAGAAG 
GTTGAGGAAC TTCAAAGCCA GAGTTTCACT AAAAACAAAG AAGTTGATCT TTTGAGAAAG 
AAAGCTCAGA AAGCGATCTT AAAGCAAGCA GATGTTGTAT GCGCCACGCT TTCCGGAAGT 
GGTCACGATT TGGTAGCACA TAGTTCTTTG AACTTTTCTA CCGTAATAAT CGATGAGGCT 
GCACAAGCTG TTGAGCTTGA TACCATCATT CCCCTTCGTT ATGGTGCAAA GAAATGTATA 
TTAGTAGGTG ATCCAAATCA ATTACCCCCT ACTGTATTAA GTAAGAAGGC GGCCAGTCTT 
AATTATTCGC AATCACTATT CGTGCGTATT CAAAAGAACT TTTCCAATCA AATGTGTCTA 
TTGAGTATTC AATATCGTAT GCACCCGGAT ATTAGCCACT TCCCGAGTAA GAAGTTCTAC 
GATTCACGAT TAGAAGATGG TGATAATATG GCTGAAAAAA CACAACAGGT TTGGCATGTT 
AATCCCAAAT TTACACAATA TCGTCTTTTT GACGTTAGAG GTAAAGAGAG AACGTCTAAT 
ACTATGTCCA CGTATAACCT GGAAGAAGTC GAGTATCTCG TCAATATGGT CGATGAATTA 
CTAAATAAAT TTCCCGACGT TAATTTCACC GGGCGAATAG GTGTTATTAC CCCATATCGA 
TCCCAACTTC ATGAACTTCG TCGAGCATTT AAAGTTAAAT ACGGAAAATC GTTTATGTCG 
ACTATTGACA TTCAGACTGT TGATGGTTTT CAGGGTCAGG AAAAAGACAT TATCTTCTTT 
TCATGTGTTA AAAGTTATTC TAAACATGGC ATTGGTTTCT TAAGAGACTT TAGGCGATTG 
AATGTTGCAT TAACACGTGC AAGAAGCTCT TTGTTAATCA TAGGAAATAT GGAAACATTG 
AAAACAGATG ACTTGTGGGG AAGCTTGGTA GATGATGCAC TTTCTCGAAA ACTGGTTGAA 
AGTCCTCACA TTGATTCTGA AGGTCGCTTG ATAACTATTT CCAGGACATC CGAAAAACGT 
ATGAAGAATG AAGAATTTGT TGAGCCACCT TCCAAAAAGC TAGCGAATTC CGAGCCTTCT 
AAAGAAATCA GACAACGATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594119.1
CDS	542..5605
Translation	MAENLSDQDCFEKLSSSKEGQHWFCSGLLTQYIQPTFFWFAHDETPSFKWVLNAYHERLRSCTSCIQAYYELRNESLAKGSYSFTGFSITDLQEKWNKWDIIRVLEDFKALEDETIDFTSLPCLIFETLLNPKLFTCKNIYKNAIDAFNGLLSDWCSNIFLPGYLLFFYEASHPDVLEWVHSFFKENTEIRISSATVDAVFNTVVFESQNSSDSDCNFVSISSPEFWERTYMFLELLPLQSITAACESILKDYLLNLVKTSESLSSQQMSCLRICCNSSSFWSAADSFKEVSSFLKNLLKNVTATPFEVSDMNWAYIIASFFRTCLNDFVSVFPEWLKGFVEEKRTIGFIIYSVLDALFDLLSLDYSSPSFLNNTLSLMNANSITILQDYPEYMLKLRLHSLLYDIAFISWSHKAITKNPSFVFDPSYELSPFWKLDNLQEEKISDVLFSKISSCCFAHDIEISENSTPSGTMVQFAELWQVMSEYISGFLKGFSEKSSTEISNMLGDSSKFDTVVSFLLSPTQPLYVSAFHIVQIITNCTKNRNEALKKLVAMDFRGIVHGLADAVLNWQSILSFFPALRIMRFLSITNKSLSSDNSAFTENDIPTLGAYWQCIWNILDLVFSNVARWSLNNPADTVKALMKLTLKFVDDLFQNDGIFIKLLAKFDSLILLGETSESLFSFIMWLKINDLELRGIVINSLCKLFTKFSNFDYLFEDRTVTFLTDFIIRKQKAHLSADQCKQLANVLTQASPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSPTPQITVKQNTTKSSSAPRMGMLEQLKQEYLTKRNFESKLKSSAVSSRKPTFNEVKPANLLAEDLSDNEDDIDRKQGLFSLAKANKIPEIRQQERRQVQLLSNSTIKMHPSQIRMMTNRNVANVKARLFPSMTDFYKEILSWEPANQSPNPVLKFHKLDGKIIDSFKTVEHYMEVLQPMIFMECWSQIQSTKLDLKFSPVEGIMVERTAVNNFVDIGVSVAPKDLYGYPLYDTEVVSLAFNKEDASSMKGLCCFAKVERIVRQTNGVLVVLRTLPSMEILNKLQGNCALWFLKLTNLATFTRQYAGIRGLPYFHLADDIIRARPCSQPVKHSSSEIKAAMKRYQVNEPQAKAIMCALDNNGFTLIQGPPGTGKTKTIIGIISALLVDLSRYHITRPNQQSKSTESKQQILLCAPSNAAVDEVLLRLKRGFLLENGEKYIPRVVRIGNPETINVSVRDLSLEYQTEKQLLEVNQGAIDLGSLQELTRWRDTFYDCIQKIEELEKQIDVARDVAEDTKSLGKELQNKINEKNLAEQKVEELQSQSFTKNKEVDLLRKKAQKAILKQADVVCATLSGSGHDLVAHSSLNFSTVIIDEAAQAVELDTIIPLRYGAKKCILVGDPNQLPPTVLSKKAASLNYSQSLFVRIQKNFSNQMCLLSIQYRMHPDISHFPSKKFYDSRLEDGDNMAEKTQQVWHVNPKFTQYRLFDVRGKERTSNTMSTYNLEEVEYLVNMVDELLNKFPDVNFTGRIGVITPYRSQLHELRRAFKVKYGKSFMSTIDIQTVDGFQGQEKDIIFFSCVKSYSKHGIGFLRDFRRLNVALTRARSSLLIIGNMETLKTDDLWGSLVDDALSRKLVESPHIDSEGRLITISRTSEKRMKNEEFVEPPSKKLANSEPSKEIRQRS

Target ORF information:

RefSeq Version	NM_001019543.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe helicase Sen1 (sen1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019543.2

ORF Insert Sequence:

ATGGCGGAGA ATCTTAGCGA TCAAGATTGT TTTGAAAAAT TATCGTCTAG TAAAGAGGGT 
CAGCATTGGT TTTGCTCAGG ACTACTGACT CAGTATATAC AACCTACCTT CTTTTGGTTT 
GCACATGATG AAACTCCGAG CTTTAAATGG GTATTAAATG CCTATCATGA GCGTTTACGC 
TCTTGTACTT CTTGTATACA AGCTTATTAT GAACTTCGAA ATGAATCCCT TGCGAAAGGT 
TCATATTCTT TTACTGGGTT TTCAATTACC GATTTACAGG AAAAGTGGAA TAAATGGGAT 
ATTATCCGTG TCTTAGAGGA CTTCAAGGCC CTGGAGGATG AAACAATTGA CTTCACGTCT 
TTGCCTTGCT TAATTTTCGA AACCCTCTTG AATCCTAAAT TGTTTACCTG TAAAAATATT 
TATAAAAATG CAATAGATGC ATTTAATGGT CTACTTTCAG ATTGGTGTTC TAATATATTT 
CTTCCTGGTT ATTTACTATT TTTCTATGAA GCTTCCCATC CCGATGTTTT GGAATGGGTT 
CATAGCTTTT TTAAGGAAAA TACTGAAATA AGAATTTCTT CTGCTACAGT TGACGCCGTT 
TTTAATACTG TAGTGTTTGA AAGTCAAAAT TCCTCTGACA GTGATTGTAA TTTCGTGTCT 
ATAAGTAGTC CTGAATTTTG GGAACGTACT TACATGTTTT TAGAACTTCT TCCTTTACAA 
TCCATTACTG CTGCATGTGA GTCCATTTTA AAAGATTATC TTTTAAATTT GGTAAAGACT 
TCTGAATCTC TATCCTCTCA ACAAATGTCC TGTCTTCGAA TTTGCTGCAA TTCCTCATCC 
TTTTGGTCTG CCGCTGATTC ATTTAAGGAA GTGTCTTCTT TCTTAAAAAA CTTGCTAAAA 
AATGTTACAG CTACACCTTT TGAAGTATCT GACATGAACT GGGCTTACAT TATTGCTTCA 
TTTTTTCGCA CATGTTTAAA TGACTTTGTT AGTGTTTTTC CGGAATGGCT TAAAGGATTT 
GTAGAAGAGA AGCGTACTAT AGGTTTCATC ATTTATTCGG TTTTGGATGC GTTATTTGAT 
CTATTATCGC TAGATTACAG CTCTCCTTCA TTTCTTAACA ATACACTATC GTTGATGAAT 
GCTAATAGCA TCACTATTTT ACAAGATTAT CCGGAGTACA TGCTGAAACT CAGATTGCAC 
TCTTTACTTT ATGACATTGC ATTTATAAGT TGGTCTCATA AGGCAATCAC TAAAAATCCC 
TCATTTGTTT TCGATCCTTC ATATGAACTC TCTCCTTTTT GGAAGCTTGA CAATCTACAG 
GAAGAAAAAA TATCTGACGT CCTTTTCTCT AAAATTTCAA GTTGTTGCTT TGCCCACGAT 
ATTGAAATAT CCGAAAATTC TACACCGTCT GGAACCATGG TGCAGTTTGC TGAACTATGG 
CAAGTAATGT CGGAATATAT CAGTGGATTT TTAAAAGGAT TTTCTGAAAA GTCGTCTACT 
GAAATTTCAA ATATGCTGGG AGATTCTTCA AAATTTGACA CAGTTGTATC ATTTCTGTTA 
TCTCCTACTC AGCCGCTCTA TGTTAGTGCT TTTCATATTG TCCAAATAAT TACCAATTGC 
ACTAAAAACA GGAATGAGGC TCTTAAAAAA CTGGTTGCAA TGGATTTTCG AGGAATTGTG 
CATGGTCTAG CTGATGCTGT TCTTAACTGG CAATCTATTT TAAGCTTCTT TCCTGCTCTT 
CGAATTATGA GATTTTTGAG CATTACTAAT AAATCCTTAT CATCTGACAA TTCTGCTTTC 
ACAGAAAATG ACATACCTAC CTTGGGTGCT TATTGGCAGT GCATCTGGAA CATATTGGAT 
TTAGTTTTTT CCAACGTTGC CAGATGGTCG CTTAACAATC CTGCTGATAC GGTTAAAGCA 
TTGATGAAAC TAACACTTAA GTTTGTTGAT GATCTTTTCC AAAATGACGG AATCTTCATA 
AAGCTCCTTG CTAAGTTTGA TAGCTTGATT CTGTTAGGCG AAACTTCTGA ATCATTGTTC 
TCTTTTATTA TGTGGCTTAA AATAAACGAT TTAGAATTAA GGGGAATTGT CATCAATAGT 
TTATGCAAGC TTTTCACTAA ATTTTCGAAT TTTGACTACC TTTTCGAGGA TCGCACCGTT 
ACCTTCCTTA CAGATTTTAT CATTAGAAAG CAAAAAGCAC ATTTGTCTGC CGATCAATGT 
AAACAGCTTG CTAATGTTTT GACTCAGGCT AGCCCTGAAG CAAAGACTGT ACTTGAGCAA 
CACCGACTTT CGGAAATGCG AAAAACAAAG AAACAAACAG AACTAACTAA TAGTGCACAT 
GTCATAAAGC CAAGTCCTAC ACCTCAAATT ACTGTGAAAC AGAATACTAC TAAATCTTCA 
AGTGCCCCTC GCATGGGAAT GCTGGAGCAA CTAAAGCAGG AGTATTTGAC TAAACGAAAC 
TTTGAATCTA AACTCAAATC ATCTGCTGTA TCTTCAAGAA AGCCAACATT TAATGAAGTC 
AAGCCAGCTA ACCTTTTAGC TGAGGACCTC TCAGATAATG AGGATGATAT TGATAGAAAG 
CAAGGATTAT TCTCTCTTGC CAAAGCAAAC AAAATTCCAG AGATACGCCA ACAAGAGCGA 
CGACAAGTGC AGCTATTATC TAATTCCACT ATTAAGATGC ATCCTTCACA AATTCGGATG 
ATGACTAATA GGAACGTTGC AAATGTTAAA GCACGCTTAT TTCCAAGTAT GACTGACTTT 
TACAAAGAAA TTTTGAGCTG GGAACCAGCT AATCAGTCAC CTAATCCTGT ACTGAAATTT 
CATAAGCTTG ATGGGAAAAT TATTGATAGT TTTAAAACGG TTGAACATTA TATGGAGGTT 
TTGCAGCCGA TGATATTTAT GGAATGCTGG AGTCAAATTC AGTCAACAAA ATTGGATTTA 
AAATTCTCTC CTGTTGAAGG AATCATGGTT GAAAGAACGG CCGTCAATAA TTTTGTTGAT 
ATAGGAGTCT CAGTGGCTCC CAAGGATCTA TACGGTTATC CCTTATATGA TACGGAAGTA 
GTTTCGTTGG CTTTTAACAA GGAGGATGCT TCATCAATGA AAGGGCTGTG TTGCTTTGCG 
AAAGTTGAAC GCATAGTTAG GCAAACGAAT GGAGTTTTAG TTGTCTTACG TACGTTACCA 
TCTATGGAAA TTTTGAACAA ATTACAAGGT AATTGTGCTT TATGGTTTCT AAAATTAACA 
AATTTGGCTA CATTCACTCG CCAATATGCC GGCATAAGAG GTTTACCATA CTTTCATCTA 
GCAGACGATA TTATTCGTGC GAGGCCTTGT TCTCAACCAG TTAAGCATTC TTCAAGCGAG 
ATAAAAGCAG CTATGAAGCG ATATCAGGTT AACGAACCTC AAGCTAAGGC CATTATGTGC 
GCTCTTGATA ATAACGGATT TACTTTGATT CAAGGTCCTC CCGGTACTGG TAAAACAAAA 
ACAATTATTG GGATTATTTC GGCTTTGCTC GTGGACCTTA GTCGATACCA CATAACACGA 
CCGAATCAAC AATCGAAGAG CACAGAATCA AAGCAACAAA TTTTACTTTG TGCACCTAGT 
AATGCAGCGG TTGACGAAGT TTTATTGCGT CTAAAACGTG GTTTCCTTTT AGAAAATGGT 
GAAAAATATA TACCAAGAGT TGTCCGTATA GGTAATCCGG AAACCATTAA TGTTTCAGTT 
CGCGATTTGT CTTTGGAATA TCAAACTGAA AAACAACTTT TAGAAGTCAA CCAAGGTGCT 
ATTGACCTTG GGTCGCTCCA GGAACTAACT CGCTGGCGTG ATACGTTTTA CGATTGTATT 
CAAAAAATCG AAGAGCTTGA AAAACAAATC GACGTTGCCC GTGATGTTGC TGAAGATACT 
AAATCTTTGG GAAAAGAACT CCAAAATAAA ATAAATGAAA AAAATTTGGC AGAACAGAAG 
GTTGAGGAAC TTCAAAGCCA GAGTTTCACT AAAAACAAAG AAGTTGATCT TTTGAGAAAG 
AAAGCTCAGA AAGCGATCTT AAAGCAAGCA GATGTTGTAT GCGCCACGCT TTCCGGAAGT 
GGTCACGATT TGGTAGCACA TAGTTCTTTG AACTTTTCTA CCGTAATAAT CGATGAGGCT 
GCACAAGCTG TTGAGCTTGA TACCATCATT CCCCTTCGTT ATGGTGCAAA GAAATGTATA 
TTAGTAGGTG ATCCAAATCA ATTACCCCCT ACTGTATTAA GTAAGAAGGC GGCCAGTCTT 
AATTATTCGC AATCACTATT CGTGCGTATT CAAAAGAACT TTTCCAATCA AATGTGTCTA 
TTGAGTATTC AATATCGTAT GCACCCGGAT ATTAGCCACT TCCCGAGTAA GAAGTTCTAC 
GATTCACGAT TAGAAGATGG TGATAATATG GCTGAAAAAA CACAACAGGT TTGGCATGTT 
AATCCCAAAT TTACACAATA TCGTCTTTTT GACGTTAGAG GTAAAGAGAG AACGTCTAAT 
ACTATGTCCA CGTATAACCT GGAAGAAGTC GAGTATCTCG TCAATATGGT CGATGAATTA 
CTAAATAAAT TTCCCGACGT TAATTTCACC GGGCGAATAG GTGTTATTAC CCCATATCGA 
TCCCAACTTC ATGAACTTCG TCGAGCATTT AAAGTTAAAT ACGGAAAATC GTTTATGTCG 
ACTATTGACA TTCAGACTGT TGATGGTTTT CAGGGTCAGG AAAAAGACAT TATCTTCTTT 
TCATGTGTTA AAAGTTATTC TAAACATGGC ATTGGTTTCT TAAGAGACTT TAGGCGATTG 
AATGTTGCAT TAACACGTGC AAGAAGCTCT TTGTTAATCA TAGGAAATAT GGAAACATTG 
AAAACAGATG ACTTGTGGGG AAGCTTGGTA GATGATGCAC TTTCTCGAAA ACTGGTTGAA 
AGTCCTCACA TTGATTCTGA AGGTCGCTTG ATAACTATTT CCAGGACATC CGAAAAACGT 
ATGAAGAATG AAGAATTTGT TGAGCCACCT TCCAAAAAGC TAGCGAATTC CGAGCCTTCT 
AAAGAAATCA GACAACGATC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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sen1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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