mok13 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	mok13
Entrez Gene ID	2540007
Full Name	alpha-1,3-glucan synthase Mok13
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022421.2	NP_596500.1	alpha-1,3-glucan synthase Mok13

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	mok11	NP_001018282.1
Schizosaccharomyces pombe (fission yeast)	ags1	NP_588165.3
Schizosaccharomyces pombe (fission yeast)	mok13	NP_596500.1
Neurospora crassa	NCU02478	XP_965072.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mok13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mok13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101247	NM_001022421.2 Latest version!	Schizosaccharomyces pombe alpha-1,3-glucan synthase Mok13 (mok13), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mok13 ( NM_001022421.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596500.1 Schizosaccharomyces pombe alpha-1,3-glucan synthase Mok13 (mok13), partial mRNA.

Price & Availability↑

CloneID	OSc101247
Clone ID Related Accession (Same CDS sequence)	NM_001022421.2
Accession Version	NM_001022421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	alpha-1,3-glucan synthase Mok13
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022421.1. COMPLETENESS: incomplete on the 5' end.

ATGCGAAATA AGAATATTCT CGTCTTGAAT TTGATATTAA GTATACCAAG GTTGGTCTTT 
ACTGCTAAAT ATGATGAAAG AGAATCTTTA TGGAATTTAA ACCAAAATCA ATCAGCTACC 
GATCCTCTAG ATTATTGGGG AAAATGGGAA AACCATCAAT ACCATCCCAG TCCAGATGAT 
TGGCAAGTGC CATTTTACAC AGTTATTTTG GATAAATGGA AAGATGGTGA TCCTAGAAAT 
AATGAAGCTA ACAATACTAT TTATGAGTAT GATATTTATG AAACTGGTTT TCGAAACGGA 
GGGGATATAA TAGGCCTGAA AGACTCACTA GATTATTTGG AGATTATGGG AATAAAGGTT 
ATCTACATCG CCGGTACACC ATTTTTAAAT CAGCCATGGG GTGCTGATCA GTACTCACCA 
CTGGACTACA CCATCCTCGA TCACCATTCT GGAACAATTG CACAATGGCG TGACACGATA 
GAAGAAATCC ATCGTAGAGG GTTTTATTTA GTTCTGGACC TTACAATTTC TACGTTGGGA 
GATCTTATAG GGTTTCGGAA GTATCTGAAC AGTACTACTC CATTTTCTTT ATTTGAACAT 
GAGGCGGTGT GGAAAAGCAA CGTCATTTAT CCAGACTGGA ATTTCACAAA TAAATATGAC 
CCAAAGTGTG AGCTTCCCAG ATTTTGGGGT GAAGATGGCG CGCCTGTTGT GATTGATTAT 
GTTGGTTGTT ATGATTCAGA TTTCGATCAG TATGGCGATA CAGAAGCCTT TGGTACTCAT 
CCTGATTGGG AACGTCAGCT TTCAAAGTTT GCTTCTGTCC AAGATAGACT TAGAGAATGG 
AGGCCATCGG TTTCAGAAAA GTTAAAACAC TTCGCATGTA TGATCATTGC CATGTTAGAT 
GTGGATGGTT TTCGTATTGA TAAAGCTACT CAAATTACAG TTGATTTTCT TGCTTCATGG 
GCTCATTCTG TGAGGGGGTG TGCAGCAACG TTTAATAAAA AGAATTTTTT TATACCTGGT 
GAAGTAACTG GTTCTTCAAG TTATGGCTCA ATTTATTACG GTCGTGGTCG TCAACCAGAT 
CAACGTCCTC CGTCGATACT TACTTCACTT AATTCATCAT CCCTTAAAGA AAACTACTTT 
CTGAGGGAAC CTAAAGCGAA TGCCCTAGAT GCTTCTGCTT TTCACTATTC ACTCTACCGT 
GCCATGACCA GATTTCTGCA AATGGACGGA GATCTGCAAG TTGGTCATGA TCTTCCCGTA 
GATTTTACAG ATTTGTGGAA TGCTATGGCA GTTAATGAAG ACTTTTATAA TCCTAACACT 
CATAAAGTTG ACCCGCGTCA CATGCTAGGG ATTACTAACC ATGATGTTTT TAGATGGTCA 
GCAATTGAAT TTGGATTAGA ACGTTTGCTA TTAGGAACCA TGATAACGTA CTTTCTTTTT 
CCAGGAGCAC CCTCCATTTA TTATGGTGAT GAACAAGGAT TCTATGTACT GGATAATACA 
GCAAACAATT ATCTTTACGG TCGCCAAGCG ATGCCTTCAT CTATAGCATG GAAAGTTCAT 
GGTTGTTATG CACTGGCATC TGATCAGTAT CCAGAACTTC CTGTCATAAA GGCATATCAA 
GGGTGTAATG ATGATTGGAA TATAATGGAC CACTTTGATT TTGCTAAACC CGAATTAAAA 
ATGTTCAAGA TATTCAACTT TATTAGGGAA CAATACCCGG CCTTAAAATC TGGATGGAAA 
TCAGTTAAAC TAAGAAATTG GACTGAATAT GTACATTTTC CTAATAGCGG AAAGACGCCT 
ACGGAAGTTG GGGTTTGGAG TATAGTCCGC GGTGCTTTAG AGACGTTGCA GAATTTTGAT 
GCCCGTAATA ATACTGCTTG GAATGGAGAC ATTTGGATAC TTTATACTAA TCAAAATAGA 
ACGACTACAC TCGATTATCA ATGCTCTTCC TCTAATTCCG TTGTGTCTCC ATATGCTTCA 
GGTTTGACTC TCAAAAATTT AATCTATCCT TTCGAAGAAT ATATACTTCA GGAATCAAAC 
AAATATTCTT CTAATCTAAA GTCCTACTAT GGGTGTATTC CTAATATAGA ATTCCCACCC 
TGGGGATTTA AAATACTTAT TCCTAAAAAA TATTACGTCC GATACCCACC ACAAATTACT 
TCTTTTAATC CCCAACATGA CTCGAGAATA TACAATCATA ATGGAAAACA AAAGCTTGTT 
ATATCATTTA CTGAAACGAT GGATTGTAAT GAAATAACAT CCAAATTGCA ATTCAGTTCG 
AAAACAGAAA GTGGTAAAGT CATGAAAGTT GATAAAGAAA CTGTGAAATG CTCAGTTTCA 
AATAATTCTG CAGATAGTTA CTATTTTGGA CTTGCGCCAG CTCGATTTCA CTGGTCAGGC 
GACTTAATTA ACATTGCTGA TGGGATTCAT GAAATAAAGC TCCAACGGGT TCATTCACAA 
GATCATCAAA GTATGAGTGA TTCCATGTAT AAACTCTTAT TAAGATTTGG TAAATTGGAT 
AATCCAATGG TATTCTCAAC TGCCAATCGA TCTTCTAGTA TATTATCACA AGAAAATGAG 
AAGTTATATA TAAACCACAA AGCCCCAGGT GCTGATCTAT TCCGATTTTC GTTCGATTAT 
GGACTTCATT GGAGTGAATG GATCGATTAC TTGTCAAATA AAACTGAGTG TACTGAGTTT 
GCTAATAACA TTAGCCTGAA AACCTGGAAA GGTCATCATG TTATTGTTCA ATACTGGAGT 
CGTTTGACGG CATCTGCGAA TTACATTCAA GAAGGTGGGC TAGGTAGCTT GAGTTCATTT 
CCTCATTTAT ATATGAATGG ACCTTATAAT CAATGGGGAT TTGATTCTGG TATACCCAAT 
CGCTTAATAT ACAAAAATTG CTCATGGCAC AAGACCTTTA TAAGTGATGT TTTTCCTACG 
AAGTTTCAGT TTAATGTGTA CAACTTTGAT GAATCTGGGA TGCCTGACCA AAAGAAAGTT 
TATGGTACTA TTGGCAACAG TACTGTACTT GTACGTCTAC CTCCGTCAGA ATTAAAAGAA 
AGTGTGACAT GGATAAAAGA AGCACCACCA TCTAACTTTT TAACTTGGGA AATTATCATA 
AGCGACTTGA CAAGAACTTA TCACCTTATA CCTAGAGGCT CATCAACTGT ATCCATTATT 
TTGTTTTCTC TTTTTTTAGT ATCACCTTTG ATATGTGCTT TAGCAACTAT GCTCGCTTTT 
CAAAAATTTT TTTATCAAGT AAGACTTAAT AAAGGGATAG AAAAAAAGCA AGAATGGAAA 
GAAAAATTAT TGGGCCCATT CAGCAGAATA AGTCAATCAA ATATCAATCA AGGATTTTCT 
CATCAAGTCG CTTTAAATAA TTCTGTCAAA AGTGTTCACC CAAAAATCTC CCGCAAATTA 
ATTCTAGTTG CTACTTTAGA GTATGATATA CCTGACTGGG ATATAAAAAT AAAAATTGGG 
GGCCTGGGAG TAATGGCAGA ACTCATGGGT AAACACTTAA CACACCATGA TCTTATTTGG 
GTGGTTCCAC GTGTTGGAGA TGTAAACTAT CCTGACGGAC AAGAATTAGC ACCTTTGGAG 
GTTGTAGTCC TTGATCAGGT TTATGAAGTG AGAGTTTATT CTCATAACTT ACGCAACATT 
ACCTATATTC TGTTAGAGGC TCCAGTCTTT AGAAAGCAAA CGTCTGCTGA ACCGTACCCT 
GCTAGAATGG ACGATTTGAG TTCTGCCATT TTCTATTCAG CTTGGAATCA GTGTATAGCC 
GGAATAATTA GACGATATCC GATAGACGTT TACCATATAA ACGATTATCA TGGTGCGTTA 
GCTCCATGTT ATCTTCTTCC AAATGTCATT CCATGCGTGT TGTCGCTTCA TAATGCTGAA 
TTCCAAGGTT TATGGCCGCT TCGCACTCAA GCCGAGAAAA ACGAAGTTTG TGCGGTATAC 
AACATTTCCA CGAAAATATG TACTAAATAC ATTCAATTCG GAAATGTTTT CAATTTACTT 
CATGCAGGAG TTTCATATAT TCGTATTCAT CAAAAAGGAT ATGGAGTAGT TGGGGTTTCT 
AATAAGTACG GGAAACGCTC AAAAGCTCGG TATCCCATAT TCTGGGGATT AAAAAAGGTA 
GGAAAACTCC CTAATCCAGA TCCACTAGAT ACTGCTCAGT TGGATGATCC CACGAATATT 
ACTGAAGAAA TCACTATTGA TTTAACGGCC GAAGCAGAGA AAAGAGCGTT TAAAAGAGAC 
GCACAAAAAT GGACGAATTT GGAACTTGAT GACTCCGCTG ATCTGCTGGT ATTTGTAGGT 
AGGTGGTCCA TGCAGAAGGG TATAGACTTA ATTGCTGACA TTGCACCTAC TCTGTTGCAA 
GATTTTAATG CTCAACTCAT TACAATAGGT CCAATAATTG ACCTGTATGG CAAATTTGCT 
GCTGAGAAAT TGAATGCTCT TATGAAAAAG TATCCGAAGC GGGTTTATTG TAGACCAGAA 
TTTACGCATT TACCACCTTG CATATTTAGT GGAGCTGACT TTGTCTTGAT CCCTTCTCGA 
GATGAACCCT TTGGGTTAGT TGCTGTTGAG TTTGGTAGAA AAGGCGCACT AGGAATAGGA 
GCTCGTGTTG GTGGTTTGGG TCAAATGCCT GGATGGTGGT ATTCAGTTGA ATCGAATGCG 
ACGTCTCATG TTTTGCAACA GTTTGAAGAA GCTTGTAGGA AGGCCCTTAG TTCTTCTGCT 
GAAAAACGGG CATTGCTTCG TGCGAAGTCA GCCAAGCAAC GGTTTCCTGT TTTAGAATGG 
ATTTCTAAAC TAGATCACCT TATGGACAAT TGTATACGTC TAAATGTCGG TCAAAGACAA 
CAAGGTTCCT CTTCACATTC TATGAAGTTT AGAAGTAAAA ATGACCTTTC CAGTATTAAA 
CTATCTACGA AGGAAGGTTT GGAAAACGAA GAAAACGAAT TGAAAGATAA AGCACCTCCA 
AATGAACCGA ATGTTGGAAG CCTCTTTTTG TTTAACAAAT CTTCTATGGG TTCTGTTGGA 
GGACCTGGTC ATTACAAAGC AACTGATTTG TCTCAAGAAT TGGAAACAAA TGATCAAGAT 
ATAGAATATA ACGAGTTTTA TTCTCAATTA GATACTAGCA CTTCTGACAT ATTTCAAGAT 
ACCTCCGTTG ATGGGTTCCC AGATCTTCAA GTGTCATCCG ATATAAACGT GCGCAACGAT 
CGTTTAAGTT CTTTTGTCAT GTCAAGTGAG GACCTGCGGT CTAGCGATGG CCATCCGGAA 
AACAGTGATT CTGTTTTAGA AACAATCAGC TCTGTTCACC ATAGAAGCCC GATCAACCAG 
GTTGTAAGGA ACCTAAATGA GTCCCAATTA AGTTTAGACT CTGTTATAAG TATGAATTTG 
AATAAGGAGT TTGCGCTGAC AAAAACTGAA AACGATTTTA CGGACGATAA TGGTCGAGCT 
CTTAATTATT TTTCCCAAAA GTTAGAAGAA CTAGATCCAA AAAATTCTGT CAATGAACTG 
TGTATTGAAA CCTTTATATT GAAAATGAAG AAAGAGTGGT ATGATGGACT TCGAAATATT 
CGTTTCGGAA TTCAACGTCC TAACCTTTTG ATTTACGACG AGGACAAAAA ATTCATAAAT 
ACAGAGCATT TTCTAGGATC CAAGGTGAAC CTTAACTCTG TGACGTCTCT TGGAAATTTT 
AATGGTTCAA GTCCTAATTC TTTTTTGTTT TTATTAAAAA ATAGAACGAT GCGGATTAAA 
TGTTTTATGC AAATGCGAAT TGGTGATTGG CCTGTTTACT CTATTTTTCT TTCAGTTGGC 
CAAATTTTGG CTGCAACATC TTATCAACTT GTTTTACTCT CAGGATCCTC TGCTCAGTTT 
TCTACACAGC TTTATATTGT AGGTAGTATT TATACTGTTT CATCTGTGTT TTGGTGGTAT 
TTATACCGCA TGCTACCTAG TGTAGCATCC CTTAGTCTTC CCTTTCTTCT TTACTGTGCC 
TCATTTCTGT TAATCGGTAT TTCGTCGTTT ATCAATGAAA ATATGTACTT GCGTTTATGG 
ATTAGTCACA TTGCCAGTTG GATATATGCT GTTGCTTCAG CCAGTGGATC ACTTTATTTT 
TCGTTAAATT TTGGTGATGA GGCTGGTGCC GGTGTCGTAT CTTGGATCGT AAGAGCATGC 
ATTGTTCAAG GCTTTCAACA AATTTGGGCT TGTTGTCTTT GGTACTGGGG ATCCTATATT 
GATCGGTCGA TGCAAGAATG TCATTCGTTT CCACATGAAG TATATCCCTT GGGTTTAATA 
GCTGTTTTTT CTTGGCCACT CGCTTTAGTG ATGTTACTAT TCGCCATTTT GTTGATTTTC 
GGTCTGCCAG ATTATTATTG GGAAAGTCCT GGAAATATTC CTGCATTTTA TACTGCCCTA 
TTAAGGAGAA AACTTGTTCT CTGGTTCTTC GTTGCAACAA TACTTCAAAA TTACTGGCTA 
TCTACCTTAT ATGGTAGATC GTGGAAATAT CTCTGGGGTG GTTCATTGTT AGCTCCATGG 
AAAATGCTGA CTATAGCATT TTTTTTGTTC TTGAGCATGT GGATCATTAT GTTGATGTTT 
CTCGGACGAA AGTCGCTTAC CCATTCTTGG CTTCTGCCGG TTTTTGGGGT AGGACTGGGA 
TCTCCCCGAT GGTTGCAAAT GATGTGGGGC ACTAGCAATA TAGGGGTTTA TCTTCCATGG 
GCTGGAGTAG CTGGTCCAAT TGTTGGTCGA ATCTTATGGA TTTGGCTGGG TGTCTTAGAT 
TCGGTACAAG GTGTTGGGGT TGGAATGATT TTATTGCAGA CACTTACTAG AAGGCACATT 
GCTACAACTC TAATTGCTGG ACAAATTATC GGAACGTTGA CTTCAATGTT AGCAAGGGCA 
ACCGCACCAA ATCGACTTGG TCCTGGACTC GTCTTTTTAG ATTTGACATC ATGGAGGTTC 
GAAGATGGTG CAAAAATATT CAGAAGTGCA CCGTTTTGGA TTTGTTTGAT TTCACAAATT 
GCTGTTAGTG CTGGTTACTT GTTGTTTTTT CGCCGAGAAA ATTTAAGTCG GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596500.1
CDS	1..7077
Translation	MRNKNILVLNLILSIPRLVFTAKYDERESLWNLNQNQSATDPLDYWGKWENHQYHPSPDDWQVPFYTVILDKWKDGDPRNNEANNTIYEYDIYETGFRNGGDIIGLKDSLDYLEIMGIKVIYIAGTPFLNQPWGADQYSPLDYTILDHHSGTIAQWRDTIEEIHRRGFYLVLDLTISTLGDLIGFRKYLNSTTPFSLFEHEAVWKSNVIYPDWNFTNKYDPKCELPRFWGEDGAPVVIDYVGCYDSDFDQYGDTEAFGTHPDWERQLSKFASVQDRLREWRPSVSEKLKHFACMIIAMLDVDGFRIDKATQITVDFLASWAHSVRGCAATFNKKNFFIPGEVTGSSSYGSIYYGRGRQPDQRPPSILTSLNSSSLKENYFLREPKANALDASAFHYSLYRAMTRFLQMDGDLQVGHDLPVDFTDLWNAMAVNEDFYNPNTHKVDPRHMLGITNHDVFRWSAIEFGLERLLLGTMITYFLFPGAPSIYYGDEQGFYVLDNTANNYLYGRQAMPSSIAWKVHGCYALASDQYPELPVIKAYQGCNDDWNIMDHFDFAKPELKMFKIFNFIREQYPALKSGWKSVKLRNWTEYVHFPNSGKTPTEVGVWSIVRGALETLQNFDARNNTAWNGDIWILYTNQNRTTTLDYQCSSSNSVVSPYASGLTLKNLIYPFEEYILQESNKYSSNLKSYYGCIPNIEFPPWGFKILIPKKYYVRYPPQITSFNPQHDSRIYNHNGKQKLVISFTETMDCNEITSKLQFSSKTESGKVMKVDKETVKCSVSNNSADSYYFGLAPARFHWSGDLINIADGIHEIKLQRVHSQDHQSMSDSMYKLLLRFGKLDNPMVFSTANRSSSILSQENEKLYINHKAPGADLFRFSFDYGLHWSEWIDYLSNKTECTEFANNISLKTWKGHHVIVQYWSRLTASANYIQEGGLGSLSSFPHLYMNGPYNQWGFDSGIPNRLIYKNCSWHKTFISDVFPTKFQFNVYNFDESGMPDQKKVYGTIGNSTVLVRLPPSELKESVTWIKEAPPSNFLTWEIIISDLTRTYHLIPRGSSTVSIILFSLFLVSPLICALATMLAFQKFFYQVRLNKGIEKKQEWKEKLLGPFSRISQSNINQGFSHQVALNNSVKSVHPKISRKLILVATLEYDIPDWDIKIKIGGLGVMAELMGKHLTHHDLIWVVPRVGDVNYPDGQELAPLEVVVLDQVYEVRVYSHNLRNITYILLEAPVFRKQTSAEPYPARMDDLSSAIFYSAWNQCIAGIIRRYPIDVYHINDYHGALAPCYLLPNVIPCVLSLHNAEFQGLWPLRTQAEKNEVCAVYNISTKICTKYIQFGNVFNLLHAGVSYIRIHQKGYGVVGVSNKYGKRSKARYPIFWGLKKVGKLPNPDPLDTAQLDDPTNITEEITIDLTAEAEKRAFKRDAQKWTNLELDDSADLLVFVGRWSMQKGIDLIADIAPTLLQDFNAQLITIGPIIDLYGKFAAEKLNALMKKYPKRVYCRPEFTHLPPCIFSGADFVLIPSRDEPFGLVAVEFGRKGALGIGARVGGLGQMPGWWYSVESNATSHVLQQFEEACRKALSSSAEKRALLRAKSAKQRFPVLEWISKLDHLMDNCIRLNVGQRQQGSSSHSMKFRSKNDLSSIKLSTKEGLENEENELKDKAPPNEPNVGSLFLFNKSSMGSVGGPGHYKATDLSQELETNDQDIEYNEFYSQLDTSTSDIFQDTSVDGFPDLQVSSDINVRNDRLSSFVMSSEDLRSSDGHPENSDSVLETISSVHHRSPINQVVRNLNESQLSLDSVISMNLNKEFALTKTENDFTDDNGRALNYFSQKLEELDPKNSVNELCIETFILKMKKEWYDGLRNIRFGIQRPNLLIYDEDKKFINTEHFLGSKVNLNSVTSLGNFNGSSPNSFLFLLKNRTMRIKCFMQMRIGDWPVYSIFLSVGQILAATSYQLVLLSGSSAQFSTQLYIVGSIYTVSSVFWWYLYRMLPSVASLSLPFLLYCASFLLIGISSFINENMYLRLWISHIASWIYAVASASGSLYFSLNFGDEAGAGVVSWIVRACIVQGFQQIWACCLWYWGSYIDRSMQECHSFPHEVYPLGLIAVFSWPLALVMLLFAILLIFGLPDYYWESPGNIPAFYTALLRRKLVLWFFVATILQNYWLSTLYGRSWKYLWGGSLLAPWKMLTIAFFLFLSMWIIMLMFLGRKSLTHSWLLPVFGVGLGSPRWLQMMWGTSNIGVYLPWAGVAGPIVGRILWIWLGVLDSVQGVGVGMILLQTLTRRHIATTLIAGQIIGTLTSMLARATAPNRLGPGLVFLDLTSWRFEDGAKIFRSAPFWICLISQIAVSAGYLLFFRRENLSRP

Target ORF information:

RefSeq Version	NM_001022421.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe alpha-1,3-glucan synthase Mok13 (mok13), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022421.2

ORF Insert Sequence:

ATGCGAAATA AGAATATTCT CGTCTTGAAT TTGATATTAA GTATACCAAG GTTGGTCTTT 
ACTGCTAAAT ATGATGAAAG AGAATCTTTA TGGAATTTAA ACCAAAATCA ATCAGCTACC 
GATCCTCTAG ATTATTGGGG AAAATGGGAA AACCATCAAT ACCATCCCAG TCCAGATGAT 
TGGCAAGTGC CATTTTACAC AGTTATTTTG GATAAATGGA AAGATGGTGA TCCTAGAAAT 
AATGAAGCTA ACAATACTAT TTATGAGTAT GATATTTATG AAACTGGTTT TCGAAACGGA 
GGGGATATAA TAGGCCTGAA AGACTCACTA GATTATTTGG AGATTATGGG AATAAAGGTT 
ATCTACATCG CCGGTACACC ATTTTTAAAT CAGCCATGGG GTGCTGATCA GTACTCACCA 
CTGGACTACA CCATCCTCGA TCACCATTCT GGAACAATTG CACAATGGCG TGACACGATA 
GAAGAAATCC ATCGTAGAGG GTTTTATTTA GTTCTGGACC TTACAATTTC TACGTTGGGA 
GATCTTATAG GGTTTCGGAA GTATCTGAAC AGTACTACTC CATTTTCTTT ATTTGAACAT 
GAGGCGGTGT GGAAAAGCAA CGTCATTTAT CCAGACTGGA ATTTCACAAA TAAATATGAC 
CCAAAGTGTG AGCTTCCCAG ATTTTGGGGT GAAGATGGCG CGCCTGTTGT GATTGATTAT 
GTTGGTTGTT ATGATTCAGA TTTCGATCAG TATGGCGATA CAGAAGCCTT TGGTACTCAT 
CCTGATTGGG AACGTCAGCT TTCAAAGTTT GCTTCTGTCC AAGATAGACT TAGAGAATGG 
AGGCCATCGG TTTCAGAAAA GTTAAAACAC TTCGCATGTA TGATCATTGC CATGTTAGAT 
GTGGATGGTT TTCGTATTGA TAAAGCTACT CAAATTACAG TTGATTTTCT TGCTTCATGG 
GCTCATTCTG TGAGGGGGTG TGCAGCAACG TTTAATAAAA AGAATTTTTT TATACCTGGT 
GAAGTAACTG GTTCTTCAAG TTATGGCTCA ATTTATTACG GTCGTGGTCG TCAACCAGAT 
CAACGTCCTC CGTCGATACT TACTTCACTT AATTCATCAT CCCTTAAAGA AAACTACTTT 
CTGAGGGAAC CTAAAGCGAA TGCCCTAGAT GCTTCTGCTT TTCACTATTC ACTCTACCGT 
GCCATGACCA GATTTCTGCA AATGGACGGA GATCTGCAAG TTGGTCATGA TCTTCCCGTA 
GATTTTACAG ATTTGTGGAA TGCTATGGCA GTTAATGAAG ACTTTTATAA TCCTAACACT 
CATAAAGTTG ACCCGCGTCA CATGCTAGGG ATTACTAACC ATGATGTTTT TAGATGGTCA 
GCAATTGAAT TTGGATTAGA ACGTTTGCTA TTAGGAACCA TGATAACGTA CTTTCTTTTT 
CCAGGAGCAC CCTCCATTTA TTATGGTGAT GAACAAGGAT TCTATGTACT GGATAATACA 
GCAAACAATT ATCTTTACGG TCGCCAAGCG ATGCCTTCAT CTATAGCATG GAAAGTTCAT 
GGTTGTTATG CACTGGCATC TGATCAGTAT CCAGAACTTC CTGTCATAAA GGCATATCAA 
GGGTGTAATG ATGATTGGAA TATAATGGAC CACTTTGATT TTGCTAAACC CGAATTAAAA 
ATGTTCAAGA TATTCAACTT TATTAGGGAA CAATACCCGG CCTTAAAATC TGGATGGAAA 
TCAGTTAAAC TAAGAAATTG GACTGAATAT GTACATTTTC CTAATAGCGG AAAGACGCCT 
ACGGAAGTTG GGGTTTGGAG TATAGTCCGC GGTGCTTTAG AGACGTTGCA GAATTTTGAT 
GCCCGTAATA ATACTGCTTG GAATGGAGAC ATTTGGATAC TTTATACTAA TCAAAATAGA 
ACGACTACAC TCGATTATCA ATGCTCTTCC TCTAATTCCG TTGTGTCTCC ATATGCTTCA 
GGTTTGACTC TCAAAAATTT AATCTATCCT TTCGAAGAAT ATATACTTCA GGAATCAAAC 
AAATATTCTT CTAATCTAAA GTCCTACTAT GGGTGTATTC CTAATATAGA ATTCCCACCC 
TGGGGATTTA AAATACTTAT TCCTAAAAAA TATTACGTCC GATACCCACC ACAAATTACT 
TCTTTTAATC CCCAACATGA CTCGAGAATA TACAATCATA ATGGAAAACA AAAGCTTGTT 
ATATCATTTA CTGAAACGAT GGATTGTAAT GAAATAACAT CCAAATTGCA ATTCAGTTCG 
AAAACAGAAA GTGGTAAAGT CATGAAAGTT GATAAAGAAA CTGTGAAATG CTCAGTTTCA 
AATAATTCTG CAGATAGTTA CTATTTTGGA CTTGCGCCAG CTCGATTTCA CTGGTCAGGC 
GACTTAATTA ACATTGCTGA TGGGATTCAT GAAATAAAGC TCCAACGGGT TCATTCACAA 
GATCATCAAA GTATGAGTGA TTCCATGTAT AAACTCTTAT TAAGATTTGG TAAATTGGAT 
AATCCAATGG TATTCTCAAC TGCCAATCGA TCTTCTAGTA TATTATCACA AGAAAATGAG 
AAGTTATATA TAAACCACAA AGCCCCAGGT GCTGATCTAT TCCGATTTTC GTTCGATTAT 
GGACTTCATT GGAGTGAATG GATCGATTAC TTGTCAAATA AAACTGAGTG TACTGAGTTT 
GCTAATAACA TTAGCCTGAA AACCTGGAAA GGTCATCATG TTATTGTTCA ATACTGGAGT 
CGTTTGACGG CATCTGCGAA TTACATTCAA GAAGGTGGGC TAGGTAGCTT GAGTTCATTT 
CCTCATTTAT ATATGAATGG ACCTTATAAT CAATGGGGAT TTGATTCTGG TATACCCAAT 
CGCTTAATAT ACAAAAATTG CTCATGGCAC AAGACCTTTA TAAGTGATGT TTTTCCTACG 
AAGTTTCAGT TTAATGTGTA CAACTTTGAT GAATCTGGGA TGCCTGACCA AAAGAAAGTT 
TATGGTACTA TTGGCAACAG TACTGTACTT GTACGTCTAC CTCCGTCAGA ATTAAAAGAA 
AGTGTGACAT GGATAAAAGA AGCACCACCA TCTAACTTTT TAACTTGGGA AATTATCATA 
AGCGACTTGA CAAGAACTTA TCACCTTATA CCTAGAGGCT CATCAACTGT ATCCATTATT 
TTGTTTTCTC TTTTTTTAGT ATCACCTTTG ATATGTGCTT TAGCAACTAT GCTCGCTTTT 
CAAAAATTTT TTTATCAAGT AAGACTTAAT AAAGGGATAG AAAAAAAGCA AGAATGGAAA 
GAAAAATTAT TGGGCCCATT CAGCAGAATA AGTCAATCAA ATATCAATCA AGGATTTTCT 
CATCAAGTCG CTTTAAATAA TTCTGTCAAA AGTGTTCACC CAAAAATCTC CCGCAAATTA 
ATTCTAGTTG CTACTTTAGA GTATGATATA CCTGACTGGG ATATAAAAAT AAAAATTGGG 
GGCCTGGGAG TAATGGCAGA ACTCATGGGT AAACACTTAA CACACCATGA TCTTATTTGG 
GTGGTTCCAC GTGTTGGAGA TGTAAACTAT CCTGACGGAC AAGAATTAGC ACCTTTGGAG 
GTTGTAGTCC TTGATCAGGT TTATGAAGTG AGAGTTTATT CTCATAACTT ACGCAACATT 
ACCTATATTC TGTTAGAGGC TCCAGTCTTT AGAAAGCAAA CGTCTGCTGA ACCGTACCCT 
GCTAGAATGG ACGATTTGAG TTCTGCCATT TTCTATTCAG CTTGGAATCA GTGTATAGCC 
GGAATAATTA GACGATATCC GATAGACGTT TACCATATAA ACGATTATCA TGGTGCGTTA 
GCTCCATGTT ATCTTCTTCC AAATGTCATT CCATGCGTGT TGTCGCTTCA TAATGCTGAA 
TTCCAAGGTT TATGGCCGCT TCGCACTCAA GCCGAGAAAA ACGAAGTTTG TGCGGTATAC 
AACATTTCCA CGAAAATATG TACTAAATAC ATTCAATTCG GAAATGTTTT CAATTTACTT 
CATGCAGGAG TTTCATATAT TCGTATTCAT CAAAAAGGAT ATGGAGTAGT TGGGGTTTCT 
AATAAGTACG GGAAACGCTC AAAAGCTCGG TATCCCATAT TCTGGGGATT AAAAAAGGTA 
GGAAAACTCC CTAATCCAGA TCCACTAGAT ACTGCTCAGT TGGATGATCC CACGAATATT 
ACTGAAGAAA TCACTATTGA TTTAACGGCC GAAGCAGAGA AAAGAGCGTT TAAAAGAGAC 
GCACAAAAAT GGACGAATTT GGAACTTGAT GACTCCGCTG ATCTGCTGGT ATTTGTAGGT 
AGGTGGTCCA TGCAGAAGGG TATAGACTTA ATTGCTGACA TTGCACCTAC TCTGTTGCAA 
GATTTTAATG CTCAACTCAT TACAATAGGT CCAATAATTG ACCTGTATGG CAAATTTGCT 
GCTGAGAAAT TGAATGCTCT TATGAAAAAG TATCCGAAGC GGGTTTATTG TAGACCAGAA 
TTTACGCATT TACCACCTTG CATATTTAGT GGAGCTGACT TTGTCTTGAT CCCTTCTCGA 
GATGAACCCT TTGGGTTAGT TGCTGTTGAG TTTGGTAGAA AAGGCGCACT AGGAATAGGA 
GCTCGTGTTG GTGGTTTGGG TCAAATGCCT GGATGGTGGT ATTCAGTTGA ATCGAATGCG 
ACGTCTCATG TTTTGCAACA GTTTGAAGAA GCTTGTAGGA AGGCCCTTAG TTCTTCTGCT 
GAAAAACGGG CATTGCTTCG TGCGAAGTCA GCCAAGCAAC GGTTTCCTGT TTTAGAATGG 
ATTTCTAAAC TAGATCACCT TATGGACAAT TGTATACGTC TAAATGTCGG TCAAAGACAA 
CAAGGTTCCT CTTCACATTC TATGAAGTTT AGAAGTAAAA ATGACCTTTC CAGTATTAAA 
CTATCTACGA AGGAAGGTTT GGAAAACGAA GAAAACGAAT TGAAAGATAA AGCACCTCCA 
AATGAACCGA ATGTTGGAAG CCTCTTTTTG TTTAACAAAT CTTCTATGGG TTCTGTTGGA 
GGACCTGGTC ATTACAAAGC AACTGATTTG TCTCAAGAAT TGGAAACAAA TGATCAAGAT 
ATAGAATATA ACGAGTTTTA TTCTCAATTA GATACTAGCA CTTCTGACAT ATTTCAAGAT 
ACCTCCGTTG ATGGGTTCCC AGATCTTCAA GTGTCATCCG ATATAAACGT GCGCAACGAT 
CGTTTAAGTT CTTTTGTCAT GTCAAGTGAG GACCTGCGGT CTAGCGATGG CCATCCGGAA 
AACAGTGATT CTGTTTTAGA AACAATCAGC TCTGTTCACC ATAGAAGCCC GATCAACCAG 
GTTGTAAGGA ACCTAAATGA GTCCCAATTA AGTTTAGACT CTGTTATAAG TATGAATTTG 
AATAAGGAGT TTGCGCTGAC AAAAACTGAA AACGATTTTA CGGACGATAA TGGTCGAGCT 
CTTAATTATT TTTCCCAAAA GTTAGAAGAA CTAGATCCAA AAAATTCTGT CAATGAACTG 
TGTATTGAAA CCTTTATATT GAAAATGAAG AAAGAGTGGT ATGATGGACT TCGAAATATT 
CGTTTCGGAA TTCAACGTCC TAACCTTTTG ATTTACGACG AGGACAAAAA ATTCATAAAT 
ACAGAGCATT TTCTAGGATC CAAGGTGAAC CTTAACTCTG TGACGTCTCT TGGAAATTTT 
AATGGTTCAA GTCCTAATTC TTTTTTGTTT TTATTAAAAA ATAGAACGAT GCGGATTAAA 
TGTTTTATGC AAATGCGAAT TGGTGATTGG CCTGTTTACT CTATTTTTCT TTCAGTTGGC 
CAAATTTTGG CTGCAACATC TTATCAACTT GTTTTACTCT CAGGATCCTC TGCTCAGTTT 
TCTACACAGC TTTATATTGT AGGTAGTATT TATACTGTTT CATCTGTGTT TTGGTGGTAT 
TTATACCGCA TGCTACCTAG TGTAGCATCC CTTAGTCTTC CCTTTCTTCT TTACTGTGCC 
TCATTTCTGT TAATCGGTAT TTCGTCGTTT ATCAATGAAA ATATGTACTT GCGTTTATGG 
ATTAGTCACA TTGCCAGTTG GATATATGCT GTTGCTTCAG CCAGTGGATC ACTTTATTTT 
TCGTTAAATT TTGGTGATGA GGCTGGTGCC GGTGTCGTAT CTTGGATCGT AAGAGCATGC 
ATTGTTCAAG GCTTTCAACA AATTTGGGCT TGTTGTCTTT GGTACTGGGG ATCCTATATT 
GATCGGTCGA TGCAAGAATG TCATTCGTTT CCACATGAAG TATATCCCTT GGGTTTAATA 
GCTGTTTTTT CTTGGCCACT CGCTTTAGTG ATGTTACTAT TCGCCATTTT GTTGATTTTC 
GGTCTGCCAG ATTATTATTG GGAAAGTCCT GGAAATATTC CTGCATTTTA TACTGCCCTA 
TTAAGGAGAA AACTTGTTCT CTGGTTCTTC GTTGCAACAA TACTTCAAAA TTACTGGCTA 
TCTACCTTAT ATGGTAGATC GTGGAAATAT CTCTGGGGTG GTTCATTGTT AGCTCCATGG 
AAAATGCTGA CTATAGCATT TTTTTTGTTC TTGAGCATGT GGATCATTAT GTTGATGTTT 
CTCGGACGAA AGTCGCTTAC CCATTCTTGG CTTCTGCCGG TTTTTGGGGT AGGACTGGGA 
TCTCCCCGAT GGTTGCAAAT GATGTGGGGC ACTAGCAATA TAGGGGTTTA TCTTCCATGG 
GCTGGAGTAG CTGGTCCAAT TGTTGGTCGA ATCTTATGGA TTTGGCTGGG TGTCTTAGAT 
TCGGTACAAG GTGTTGGGGT TGGAATGATT TTATTGCAGA CACTTACTAG AAGGCACATT 
GCTACAACTC TAATTGCTGG ACAAATTATC GGAACGTTGA CTTCAATGTT AGCAAGGGCA 
ACCGCACCAA ATCGACTTGG TCCTGGACTC GTCTTTTTAG ATTTGACATC ATGGAGGTTC 
GAAGATGGTG CAAAAATATT CAGAAGTGCA CCGTTTTGGA TTTGTTTGAT TTCACAAATT 
GCTGTTAGTG CTGGTTACTT GTTGTTTTTT CGCCGAGAAA ATTTAAGTCG GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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mok13 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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