mok11 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	mok11
Entrez Gene ID	3361410
Full Name	alpha-1,3-glucan synthase Mok11
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019981.2	NP_001018282.1	alpha-1,3-glucan synthase Mok11

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	mok11	NP_001018282.1
Schizosaccharomyces pombe (fission yeast)	ags1	NP_588165.3
Schizosaccharomyces pombe (fission yeast)	mok13	NP_596500.1
Neurospora crassa	NCU02478	XP_965072.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mok11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mok11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102055	NM_001019981.2 Latest version!	Schizosaccharomyces pombe alpha-1,3-glucan synthase Mok11 (mok11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mok11 ( NM_001019981.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001018282.1 Schizosaccharomyces pombe alpha-1,3-glucan synthase Mok11 (mok11), mRNA.

Price & Availability↑

CloneID	OSc102055
Clone ID Related Accession (Same CDS sequence)	NM_001019981.2
Accession Version	NM_001019981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	alpha-1,3-glucan synthase Mok11
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019981.1.

ATGGCGTACC CTCTTGTTGT TTCTTTCTTT TTGTATATTG TTATACTTGA CAAACATGCT 
TGGTGCGCTC CTTTCAATAA TCTCCTTACT GATTGGAATT TGAACACTAA CGTTTCTGCT 
CTTGATCCAT CGGATTATTG GGGTGAATGG GAGAATCATG AATTTTTTCC TAGCCCAGAA 
CATTGGAGAT TTCCCATTTA TACTATTGCT ATTGACAAAT GGGTCGATGG AGATCCAACC 
AATAATGATT TTAGTGGAAC AAGATTTGAA TACGACATTT ATGAGACAGA ATTTAGAAAC 
GGTGGAGATA TAATTGGTGT GCGTCAATCT TTAGACTATT TGCAAGGTAT GGGTGTTAAA 
GCTGTTTACT TTGCTGGGAC ACCTTTTGTT AATATGCCTT GGGGAGCTGA TCAGTATTCT 
CCTCTTGATT TCACGTTACT TGATCCTCAT TTAGGGACGA TTAACGACTG GAGAGGAACA 
ATCGAAGAAA TGCATAGTAA AGGCATGTAT GTAATTGTGG ATCTCACCGT TGCTACATTA 
GCCGATCTGA TAGGTTTCGA GGGTTTCACA AACACTACTA CTCCGTTTAC TTTTATTGAG 
CATAACGCTC TTTGGAAAGG AGAAGATCGG TATGCTGATT GGAATTTCAC AAATTCGTGG 
GACCCTGACT GTGAATTGCC TAGATTTTGG GGCGAATCTG GTGAACCTGT GGTAGTAGAA 
TGGACGGGTT GTTATAATTC GGACTTTGAC CAGTATGGTG ATACTGAAGC GTTTGGTAGT 
CATCCTGATT GGCAACGTCA ACTCTCAAAA TTTGCATCTG TTCAGGACCG GCTTCGTGAA 
TGGAAACCTT CGGTAGCATC GAAACTCAAG AGGCTTTCAT GCTTGGTGAT TTCTATGCTT 
GATGTAGACG GTTTTCGCAT TGATAAGGCT ACCCAGATGA CAGTAGATTT CCTTGTAGAT 
TGGGCCAAAA GCGTTCGTCT CTGCGCAAAT CGATTTAACA AAAGTAATTT TTTTATACCT 
GGAGAAGTCA CTGGTCCCTC AAGCTTTGGA GCTATATACT ATAACCGGGG TAGACAGCCA 
AATCAACGTC CCGCAAATTT AATCGATGCG CTGAATGCTA CTTCTTCTGA TAATGTTTAC 
TTCCTAAGAG AAGAAGGTGA AAATGCTTTG GATGCATCCG CTTTTCATTA TTCAGTTTAC 
AGGATTATTT TACGATTCTT GAGAATGGAT GGTTTAATGG AGATCCCTTA CGATCTCCCT 
GTAGATTTGG CTGAAGCATG GCACCAAATT GTGATAAATG AAGATTCTTT CAATCCCAAA 
ACTGAAAAAT ATGACCCTCG CCATTTATAC GGTGTTTCAA ATTACGATGT TTTCCGCTGG 
GCATCGGTTG CTGATGGCTC TCGCCGATTA ATCTTAGGAA CAATGATGAC ATTTTTTTTG 
TTTCCTGGTG CGCCTCTAAT ATATTATGGT GATGAACAAG GACTGTACGT TTTAGATAAC 
AGTGCAAACA ATTATTTATA TGGCAGACAA TCCATGGCTG CGGCTCCGGC TTGGTATATT 
CATGGCTGTT ATTCTGGATC ATCTTCTACT TACCCTGCTA TTGACTTAAG TCCCGCGAAA 
ATTGGTTGTT TAGATATTTG GAATTCTTTA GATCATTTTG ACCCTTCAAG AATTGAGCGA 
CAATTATTTA TAGAGTTTCA AGATATCAGG TCTCGATATA GTGCGCTCAC TCACGGCTGG 
AAATCGGAAC TTTTAGGAAA CTGGACCAAT ATTGAATATC TGCCAAATAG CGGCATAAAT 
CCCTCCAACG TTGGTACGTT TAGTATGGTG AGGGGTGCCA TTAATTCATT GCAAAATATA 
AGCTCAGAAT ACAATTTTCC TGGAGTTAAA ACATCTTCCT CAGACGTTTG GATATTGTTT 
ACAAATTCAA ACGTGAGCGT GAATTTGAAA TCAAATTGCT TTTCAAAAGA AGCCATTGTA 
TCCCCGTTTA TTAGTGGTAC AAAAATTAAG AACTTGGTTT ACCCCTATGA TGAGTATCAA 
CTTGAGGCGT CTTCTCATTT TTCACAAATT TCGAATACAG AGCCTATGGG GTGCTTGCCA 
GAGCTAGGAT TGGATGGGTA TGGTTATAAA CTGTTTGTTC CGATTAATGA ATATATTCCT 
CGTCGACCGT TTATTACGAA ATTTTCTCCA AGCCATGATT CAAGATTAGT AATCCCATTA 
GAAAAATTGA GGATAATTGT AGAGTTTTCT GAAGAAATGG ATTGCAAATC AATTTCAAAG 
TCGTTGTTAA TTACATCCAA AACTCTGAAT GGGGATTCGC CTGTACTTGA TGAGAGTTCA 
GTAACTTGCC AAAAGATAAA TGACAAAGAC CGAGTAAGGT TTTCAGGGCA ATCAAGCAGT 
TTGTTTCGCT GGTCGGCAAC CTTCTCCAAT ATTGCCGATG GAATCCATCG GATTTCCTTT 
AATAATGCTA GTACAGCTGA CGGCAAAGAT TTCACTCATT CGGTGGACCA TTTTCTACTT 
CGCGTGGGAA GTTTAAATAA TCCTATAGTT TTTTCTTCTG CTAATTATTC ATATGATTTG 
CTACAAAAGG AGAATAACTC AGTATATATT AAACATGCGG CTATAGGTGC TGATATGTTC 
CGTTATTCAT TAGATTTTGG CTCTACGTGG ACGGAATGGC AAACTTACGA TGGAAACAAT 
ACTTATTGCA ATTTATCTGG TTGGAGTCAA AGCAGTCGCT ATGGTTGGAA AGGCCATCAT 
ATAATGGTTC AGTATTGGAG TGAGTTGACA GGATCTTCCA ATTATATGCA AGAGTCGGAC 
CTTGAATATC CCTATAAACG ATGGTTTCCT CATGTGTTTA TGGACAGTGA TTATACGCAG 
TGGACTCATG ATTCAGACAT ACGCAATAGA ATGCTGCCTT TGGAAAATGG AAGTTTCTTG 
GGATATCACA TAGCAGACGT CTATCCATCA GCTCTCCAGT TCAATGTGTG GGGATTGAAT 
AAGGATGGTA AAGCCGATAA ATCATTCATT TATGGTAGTC TTCAAAACAA CAGTTTTCTA 
TCGAGAGTTA GTCCCTCATC TCTTGAAGAA AATGTTTTCT ATATTCAGCA TCCTCCACCA 
AAGTCATATT TATCTTGGTC TGTTACATTC GATCCTCAAA GGAGGCGTTA TTACATTAAT 
CCTTCGGGAT GTGTGTTTGT TTCACTTGGA ATATATCTTT CTTCTTTGAT TGTTCCATTA 
CTAACTGGAG TTCTGGCTGT TTATATATTT AAGAAGAAAT TTTATCATGT GAAGTTTAAT 
GCATTTGGAA CAAGTAAGCA AAATTTGCAT TTTCGCCAAC ATGATGTTTT AGGAATGAAA 
AATTTATTCA ACTTTTCCTG CTCAAACAAA ATCAAAGGAG AAGAAGTTTT GAACGATGGC 
GACAAAGGCA AAAGAAGGAC TGTTCTTCTT GCAACACTTG AATACGATAT CCCTTCTTTG 
AATATTTGCA TCAAAATTGG CGGATTGGGC GTCATGGCGC AACTAATGGC CCGACATTTA 
GAACATGAAG ACATCATTTG GGTGATACCT TGTGTCGGGG ATGTGTCTTA CTCAAACGTT 
GAAGAAGATG ATCCTATCGA AGTAGTAATC ATCGACCAAA CGTATTTCAT TAACGTTTAT 
AAATATGTGA TCGGAAACAT AATATACATA CTCCTTGACG CTCCAATTTT CCGAAGACAA 
ACAAGCGGAA AACCGTATCC ATCTCGTGCT GACGATCTAA GCTCGGCTAT TTTCTATTCT 
GCTTGGAATC AATGCATTGC TAGTGTAATA TCAAGAAACA ATATCGATTT ATATCACATG 
AATGATTATC ACGGTTCTCT TGCTCCGCTA TATTTGCTTC CAAAAATAAT CCCAGTGGCA 
CTGTCTTTGC ACAATGCTGA ATTCCAAGGA CTTTGGCCCC TTAGGAACTC TTCTGAGAAA 
GAGGAGGTTT GTTCGGTTTT CAATATATCT AAATCTGTTT GTTCTAAATA TGTTCAATTT 
GGTAATGTTT TTAATCTGTT GCATGCTGGC GCCTCATATA TTCGAATTCA TCAAAAAGGC 
TACGGAGTGG TGGGTGTTTC CAGTAAATAC GGAAAACGAT CTTGGGCTAG ATATCCCATA 
TTTTGGGGAT TGAGGAAAAT CGGTAAACTC CCAAATCCCG ACCCAGCAGA TAATGGTACC 
AATTTTAAAG ACCTTGACGC GAATTCCATG AATGAGTTTG AAAACATCAA AGCAAAACAT 
AAGCGTTCAG CTCAAGAATG GGCAAATCTC AATATTGATC CTGAGGCAGA TCTTTTGATA 
TTTGTGGGTC GATGGACGTT GCAAAAGGGC ATTGATTTAA TTGCAGACAT CACTCCTACC 
CTATTAGAAA ATTTCAATTC CCAAATTGTT GTGGTTGGCC CTGTCATTGA TCTTTATGGA 
AAATTTGCTG CTGAAAAGTT CACGGCTTTA ATGAAGAAGT ATCCTGGTCG AATATACTCT 
AGGCCTCTCT TTACTCAACT TCCTTCTTAC ATATTCAGTG GCGCAGATTT TGCTTTGATA 
CCCTCAAGAG ATGAACCTTT TGGTTTGGTG GCTGTTGAAT TTGGTAGGAA AGGAACATTG 
GGTATTGGTG CCAAAGTTGG AGGCCTTGGA CAGATGCCGG GATGGTGGTA TACCATCGAA 
TCTAACACAA CTGCACATTT GCTCTGCCAG TTTGAAGAAG CCTGTCGTCA AGCCTTAACA 
TCTTCAAAGT CTGTGCGCAC CAAACTTCGA GCTATTTCTA CTATTCAGCG GTTTCCTGTT 
TCTGAATGGG TCTCAAAGCT TGATACTCAT GTGAGAAATT GTATCAAGTT CAGCCATAAA 
CAAAACTTAG AAGAAGACTT TATTCATGAA CCTGTAATAG ATGTTGATGA ATTTGCGATA 
TCGTCATCAA AAGATATTGA TGCAGATGAA GATTTAGAAA TTATCGGAAG CTCAGATAAC 
AAAGCGATTG ATTCAAACGG TGAAGGATTT TTAATTGAAA AAGATAATAT TGGAACGGGA 
AGCTACTCAA ATCAACAATC ATTTGACTTT AAAAGTAGCG AAAGTGATTC ATTTCCCCAG 
AAAAGCCCTT CGGTCGAAAG TTTTTCTATA ATTGATAACG ATAATCCTTT CCACGAAGGA 
CAAAATTCTT CTACTGGTTA CAAGGACATT GTGCAAGGGC TTTTGGCTGA AAATGGTGTT 
TCAGAAGCTG GAGTTGACGT TATGACTTCT ATAGTTTCAT CCACTATTCC AATTGTATCA 
AACCATCAAA CTGAAGGTTC TCAAATGTTT AATGAAATCT CATCTGTGTC GTCTATTCAT 
GTATACCATG ATGAATCACA ACCGCCAGTT GAAATGCCAG CTGAAAGTGA CACTCCCTTA 
CAAAATAAAT TATACCATCC AGGGATGTGG TCGTCTTCTG ACATTAGAAT ACCAAATAAT 
AGCTCTCAAT TGAGTATTGA TTCAGTTCGA TCCGGTATGC GACCATTTTC ACTTTCCAAG 
GTACCTCATC AATTTGATGA TGAGGAGGGA AAAGCGCTTC AAATATTTCG AGAGAAATTA 
AAAGATCTTA ATTGCAAAAA TTCTATGAAC GAAATGTGTA TTGAAAATTT TCTCATGAAA 
TGTACTAGAA AATATTTTGA TGAAGTCCGA AAATTACGAC TTGGTACTTT AAAACCTGAA 
AATCTTCAAT TTGTTAAGGA CCCATCTAGT TTGGCTTTGG AAACTAACCT TTTACCAGCA 
TCCGACACAA TTGAGGAAAA GAACGACGTA GGTAATAAAC AGGTGAATTC GCATATATTA 
GATCCAGGGT TTCTCAAAGA AGAAGAATGT GTATATGAAT TTGAGCAACT TCATGGACTT 
CGAAAGGCTT TACAAGTTGA AATTTATGGA TGGCCATTAT ATACAATTTT GCTAGCTATT 
GGACAAGTGT TAGCTGCTAC CTCTTTTCAA CTGAATTTAT TTTCAGATAC GCCTGATCAG 
CCAGAATACC AATCCTATAT CGTGTGTAGT GTTTATATCA GTGCATCTCT ATTCTGGTAT 
ATTTTGCATT CTCTCGTACC CAATATTTAC GCACTTTCAA CCCCATTCAT AATTTACGCT 
ACTGCGTTTG CTTTAGCTGG CCTTACAACG TTTTCAAGCC TCGGTGATTC ACGTCTTTGG 
ATTTCACGAG TTGCAACCTG GATTTATTCA ATTGCCTCTG GGAGTCAAGC TTTGTTTTTT 
TCTTTAAATT TTGGTAATGA AGGAAGATAT GATATCTTGT ATTGGATTGT GAGAGCTTGT 
TTTATTCAAG GATCGCAACA AGTATGGTCG GCAGCACTTT GGTATTGGGG TTCTTACTCA 
ACGGATAAAC CAAATAGAAT GGGAGTTTCG GGAAAATTAA ACCCACAGCC ATGGATGGCA 
GCCATTACAT GGCCAATAGC TTTGGTTTTA CTCGCGATCG CTTTTGTTGT GTATCGAGGT 
TTGCCTAATT TTTACAGACA ATGTCCTTCA AAAATACCAG CTTTCTATCG TTCCTTGTTT 
CGCCGTCGGT TGATAATGTG GTTTTTTATT AGCGTTTTTC TTCAAAATTA CTGGATGTCA 
TCGGTGTATG GTCGATCGTG GGCCTTTATG TGGTCTGCAC ATAATGTACA TAAATGGGCT 
ATGTTTTTAC TAGTCATTTT GTTTTATGTT GCTATATGGA TTGCTTTGGT GGCATTACTT 
GCTTCTTTGT CCAGATACCA TTCTTGGATC CTACCTATTT TGGGATTAGG ATTTGGAGCT 
CCTCGCTGGT TACAAACATT ATGGGGCACG TCAAATATTG GCATATACGT ACCGTTTTTA 
GGAAAAGGTG CACCCTATTT ATCAAGAATG CTGTGGCTCT ATCTTGGCTT ATTGGATACA 
GTTCAAACTG TCGGTATTGG AATTATATTA TTGCAGACAC TAACAAGAGA GCACATTACT 
GTTGTTTTAA TAACCGGGCA AATTGTCGGT GCTATTGCAA GTATGGTAGG TAAAGCCAGC 
TCCCCATCAA AATTTGGACC AGGGGATGTC TTTATAGATT TCACACATTG GAGCGTTAAG 
GATGGTCCCC AGATCCTTGC AAGCATTCCA TTTTGGGTAT GCTTGATTTG TCAATTATCT 
GTTATTATCG GTTACTTCCT GTTCTTTCGC CGTGAAAATT TAAGTCGACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001018282.1
CDS	199..7392
Translation	MAYPLVVSFFLYIVILDKHAWCAPFNNLLTDWNLNTNVSALDPSDYWGEWENHEFFPSPEHWRFPIYTIAIDKWVDGDPTNNDFSGTRFEYDIYETEFRNGGDIIGVRQSLDYLQGMGVKAVYFAGTPFVNMPWGADQYSPLDFTLLDPHLGTINDWRGTIEEMHSKGMYVIVDLTVATLADLIGFEGFTNTTTPFTFIEHNALWKGEDRYADWNFTNSWDPDCELPRFWGESGEPVVVEWTGCYNSDFDQYGDTEAFGSHPDWQRQLSKFASVQDRLREWKPSVASKLKRLSCLVISMLDVDGFRIDKATQMTVDFLVDWAKSVRLCANRFNKSNFFIPGEVTGPSSFGAIYYNRGRQPNQRPANLIDALNATSSDNVYFLREEGENALDASAFHYSVYRIILRFLRMDGLMEIPYDLPVDLAEAWHQIVINEDSFNPKTEKYDPRHLYGVSNYDVFRWASVADGSRRLILGTMMTFFLFPGAPLIYYGDEQGLYVLDNSANNYLYGRQSMAAAPAWYIHGCYSGSSSTYPAIDLSPAKIGCLDIWNSLDHFDPSRIERQLFIEFQDIRSRYSALTHGWKSELLGNWTNIEYLPNSGINPSNVGTFSMVRGAINSLQNISSEYNFPGVKTSSSDVWILFTNSNVSVNLKSNCFSKEAIVSPFISGTKIKNLVYPYDEYQLEASSHFSQISNTEPMGCLPELGLDGYGYKLFVPINEYIPRRPFITKFSPSHDSRLVIPLEKLRIIVEFSEEMDCKSISKSLLITSKTLNGDSPVLDESSVTCQKINDKDRVRFSGQSSSLFRWSATFSNIADGIHRISFNNASTADGKDFTHSVDHFLLRVGSLNNPIVFSSANYSYDLLQKENNSVYIKHAAIGADMFRYSLDFGSTWTEWQTYDGNNTYCNLSGWSQSSRYGWKGHHIMVQYWSELTGSSNYMQESDLEYPYKRWFPHVFMDSDYTQWTHDSDIRNRMLPLENGSFLGYHIADVYPSALQFNVWGLNKDGKADKSFIYGSLQNNSFLSRVSPSSLEENVFYIQHPPPKSYLSWSVTFDPQRRRYYINPSGCVFVSLGIYLSSLIVPLLTGVLAVYIFKKKFYHVKFNAFGTSKQNLHFRQHDVLGMKNLFNFSCSNKIKGEEVLNDGDKGKRRTVLLATLEYDIPSLNICIKIGGLGVMAQLMARHLEHEDIIWVIPCVGDVSYSNVEEDDPIEVVIIDQTYFINVYKYVIGNIIYILLDAPIFRRQTSGKPYPSRADDLSSAIFYSAWNQCIASVISRNNIDLYHMNDYHGSLAPLYLLPKIIPVALSLHNAEFQGLWPLRNSSEKEEVCSVFNISKSVCSKYVQFGNVFNLLHAGASYIRIHQKGYGVVGVSSKYGKRSWARYPIFWGLRKIGKLPNPDPADNGTNFKDLDANSMNEFENIKAKHKRSAQEWANLNIDPEADLLIFVGRWTLQKGIDLIADITPTLLENFNSQIVVVGPVIDLYGKFAAEKFTALMKKYPGRIYSRPLFTQLPSYIFSGADFALIPSRDEPFGLVAVEFGRKGTLGIGAKVGGLGQMPGWWYTIESNTTAHLLCQFEEACRQALTSSKSVRTKLRAISTIQRFPVSEWVSKLDTHVRNCIKFSHKQNLEEDFIHEPVIDVDEFAISSSKDIDADEDLEIIGSSDNKAIDSNGEGFLIEKDNIGTGSYSNQQSFDFKSSESDSFPQKSPSVESFSIIDNDNPFHEGQNSSTGYKDIVQGLLAENGVSEAGVDVMTSIVSSTIPIVSNHQTEGSQMFNEISSVSSIHVYHDESQPPVEMPAESDTPLQNKLYHPGMWSSSDIRIPNNSSQLSIDSVRSGMRPFSLSKVPHQFDDEEGKALQIFREKLKDLNCKNSMNEMCIENFLMKCTRKYFDEVRKLRLGTLKPENLQFVKDPSSLALETNLLPASDTIEEKNDVGNKQVNSHILDPGFLKEEECVYEFEQLHGLRKALQVEIYGWPLYTILLAIGQVLAATSFQLNLFSDTPDQPEYQSYIVCSVYISASLFWYILHSLVPNIYALSTPFIIYATAFALAGLTTFSSLGDSRLWISRVATWIYSIASGSQALFFSLNFGNEGRYDILYWIVRACFIQGSQQVWSAALWYWGSYSTDKPNRMGVSGKLNPQPWMAAITWPIALVLLAIAFVVYRGLPNFYRQCPSKIPAFYRSLFRRRLIMWFFISVFLQNYWMSSVYGRSWAFMWSAHNVHKWAMFLLVILFYVAIWIALVALLASLSRYHSWILPILGLGFGAPRWLQTLWGTSNIGIYVPFLGKGAPYLSRMLWLYLGLLDTVQTVGIGIILLQTLTREHITVVLITGQIVGAIASMVGKASSPSKFGPGDVFIDFTHWSVKDGPQILASIPFWVCLICQLSVIIGYFLFFRRENLSRP

Target ORF information:

RefSeq Version	NM_001019981.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe alpha-1,3-glucan synthase Mok11 (mok11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019981.2

ORF Insert Sequence:

ATGGCGTACC CTCTTGTTGT TTCTTTCTTT TTGTATATTG TTATACTTGA CAAACATGCT 
TGGTGCGCTC CTTTCAATAA TCTCCTTACT GATTGGAATT TGAACACTAA CGTTTCTGCT 
CTTGATCCAT CGGATTATTG GGGTGAATGG GAGAATCATG AATTTTTTCC TAGCCCAGAA 
CATTGGAGAT TTCCCATTTA TACTATTGCT ATTGACAAAT GGGTCGATGG AGATCCAACC 
AATAATGATT TTAGTGGAAC AAGATTTGAA TACGACATTT ATGAGACAGA ATTTAGAAAC 
GGTGGAGATA TAATTGGTGT GCGTCAATCT TTAGACTATT TGCAAGGTAT GGGTGTTAAA 
GCTGTTTACT TTGCTGGGAC ACCTTTTGTT AATATGCCTT GGGGAGCTGA TCAGTATTCT 
CCTCTTGATT TCACGTTACT TGATCCTCAT TTAGGGACGA TTAACGACTG GAGAGGAACA 
ATCGAAGAAA TGCATAGTAA AGGCATGTAT GTAATTGTGG ATCTCACCGT TGCTACATTA 
GCCGATCTGA TAGGTTTCGA GGGTTTCACA AACACTACTA CTCCGTTTAC TTTTATTGAG 
CATAACGCTC TTTGGAAAGG AGAAGATCGG TATGCTGATT GGAATTTCAC AAATTCGTGG 
GACCCTGACT GTGAATTGCC TAGATTTTGG GGCGAATCTG GTGAACCTGT GGTAGTAGAA 
TGGACGGGTT GTTATAATTC GGACTTTGAC CAGTATGGTG ATACTGAAGC GTTTGGTAGT 
CATCCTGATT GGCAACGTCA ACTCTCAAAA TTTGCATCTG TTCAGGACCG GCTTCGTGAA 
TGGAAACCTT CGGTAGCATC GAAACTCAAG AGGCTTTCAT GCTTGGTGAT TTCTATGCTT 
GATGTAGACG GTTTTCGCAT TGATAAGGCT ACCCAGATGA CAGTAGATTT CCTTGTAGAT 
TGGGCCAAAA GCGTTCGTCT CTGCGCAAAT CGATTTAACA AAAGTAATTT TTTTATACCT 
GGAGAAGTCA CTGGTCCCTC AAGCTTTGGA GCTATATACT ATAACCGGGG TAGACAGCCA 
AATCAACGTC CCGCAAATTT AATCGATGCG CTGAATGCTA CTTCTTCTGA TAATGTTTAC 
TTCCTAAGAG AAGAAGGTGA AAATGCTTTG GATGCATCCG CTTTTCATTA TTCAGTTTAC 
AGGATTATTT TACGATTCTT GAGAATGGAT GGTTTAATGG AGATCCCTTA CGATCTCCCT 
GTAGATTTGG CTGAAGCATG GCACCAAATT GTGATAAATG AAGATTCTTT CAATCCCAAA 
ACTGAAAAAT ATGACCCTCG CCATTTATAC GGTGTTTCAA ATTACGATGT TTTCCGCTGG 
GCATCGGTTG CTGATGGCTC TCGCCGATTA ATCTTAGGAA CAATGATGAC ATTTTTTTTG 
TTTCCTGGTG CGCCTCTAAT ATATTATGGT GATGAACAAG GACTGTACGT TTTAGATAAC 
AGTGCAAACA ATTATTTATA TGGCAGACAA TCCATGGCTG CGGCTCCGGC TTGGTATATT 
CATGGCTGTT ATTCTGGATC ATCTTCTACT TACCCTGCTA TTGACTTAAG TCCCGCGAAA 
ATTGGTTGTT TAGATATTTG GAATTCTTTA GATCATTTTG ACCCTTCAAG AATTGAGCGA 
CAATTATTTA TAGAGTTTCA AGATATCAGG TCTCGATATA GTGCGCTCAC TCACGGCTGG 
AAATCGGAAC TTTTAGGAAA CTGGACCAAT ATTGAATATC TGCCAAATAG CGGCATAAAT 
CCCTCCAACG TTGGTACGTT TAGTATGGTG AGGGGTGCCA TTAATTCATT GCAAAATATA 
AGCTCAGAAT ACAATTTTCC TGGAGTTAAA ACATCTTCCT CAGACGTTTG GATATTGTTT 
ACAAATTCAA ACGTGAGCGT GAATTTGAAA TCAAATTGCT TTTCAAAAGA AGCCATTGTA 
TCCCCGTTTA TTAGTGGTAC AAAAATTAAG AACTTGGTTT ACCCCTATGA TGAGTATCAA 
CTTGAGGCGT CTTCTCATTT TTCACAAATT TCGAATACAG AGCCTATGGG GTGCTTGCCA 
GAGCTAGGAT TGGATGGGTA TGGTTATAAA CTGTTTGTTC CGATTAATGA ATATATTCCT 
CGTCGACCGT TTATTACGAA ATTTTCTCCA AGCCATGATT CAAGATTAGT AATCCCATTA 
GAAAAATTGA GGATAATTGT AGAGTTTTCT GAAGAAATGG ATTGCAAATC AATTTCAAAG 
TCGTTGTTAA TTACATCCAA AACTCTGAAT GGGGATTCGC CTGTACTTGA TGAGAGTTCA 
GTAACTTGCC AAAAGATAAA TGACAAAGAC CGAGTAAGGT TTTCAGGGCA ATCAAGCAGT 
TTGTTTCGCT GGTCGGCAAC CTTCTCCAAT ATTGCCGATG GAATCCATCG GATTTCCTTT 
AATAATGCTA GTACAGCTGA CGGCAAAGAT TTCACTCATT CGGTGGACCA TTTTCTACTT 
CGCGTGGGAA GTTTAAATAA TCCTATAGTT TTTTCTTCTG CTAATTATTC ATATGATTTG 
CTACAAAAGG AGAATAACTC AGTATATATT AAACATGCGG CTATAGGTGC TGATATGTTC 
CGTTATTCAT TAGATTTTGG CTCTACGTGG ACGGAATGGC AAACTTACGA TGGAAACAAT 
ACTTATTGCA ATTTATCTGG TTGGAGTCAA AGCAGTCGCT ATGGTTGGAA AGGCCATCAT 
ATAATGGTTC AGTATTGGAG TGAGTTGACA GGATCTTCCA ATTATATGCA AGAGTCGGAC 
CTTGAATATC CCTATAAACG ATGGTTTCCT CATGTGTTTA TGGACAGTGA TTATACGCAG 
TGGACTCATG ATTCAGACAT ACGCAATAGA ATGCTGCCTT TGGAAAATGG AAGTTTCTTG 
GGATATCACA TAGCAGACGT CTATCCATCA GCTCTCCAGT TCAATGTGTG GGGATTGAAT 
AAGGATGGTA AAGCCGATAA ATCATTCATT TATGGTAGTC TTCAAAACAA CAGTTTTCTA 
TCGAGAGTTA GTCCCTCATC TCTTGAAGAA AATGTTTTCT ATATTCAGCA TCCTCCACCA 
AAGTCATATT TATCTTGGTC TGTTACATTC GATCCTCAAA GGAGGCGTTA TTACATTAAT 
CCTTCGGGAT GTGTGTTTGT TTCACTTGGA ATATATCTTT CTTCTTTGAT TGTTCCATTA 
CTAACTGGAG TTCTGGCTGT TTATATATTT AAGAAGAAAT TTTATCATGT GAAGTTTAAT 
GCATTTGGAA CAAGTAAGCA AAATTTGCAT TTTCGCCAAC ATGATGTTTT AGGAATGAAA 
AATTTATTCA ACTTTTCCTG CTCAAACAAA ATCAAAGGAG AAGAAGTTTT GAACGATGGC 
GACAAAGGCA AAAGAAGGAC TGTTCTTCTT GCAACACTTG AATACGATAT CCCTTCTTTG 
AATATTTGCA TCAAAATTGG CGGATTGGGC GTCATGGCGC AACTAATGGC CCGACATTTA 
GAACATGAAG ACATCATTTG GGTGATACCT TGTGTCGGGG ATGTGTCTTA CTCAAACGTT 
GAAGAAGATG ATCCTATCGA AGTAGTAATC ATCGACCAAA CGTATTTCAT TAACGTTTAT 
AAATATGTGA TCGGAAACAT AATATACATA CTCCTTGACG CTCCAATTTT CCGAAGACAA 
ACAAGCGGAA AACCGTATCC ATCTCGTGCT GACGATCTAA GCTCGGCTAT TTTCTATTCT 
GCTTGGAATC AATGCATTGC TAGTGTAATA TCAAGAAACA ATATCGATTT ATATCACATG 
AATGATTATC ACGGTTCTCT TGCTCCGCTA TATTTGCTTC CAAAAATAAT CCCAGTGGCA 
CTGTCTTTGC ACAATGCTGA ATTCCAAGGA CTTTGGCCCC TTAGGAACTC TTCTGAGAAA 
GAGGAGGTTT GTTCGGTTTT CAATATATCT AAATCTGTTT GTTCTAAATA TGTTCAATTT 
GGTAATGTTT TTAATCTGTT GCATGCTGGC GCCTCATATA TTCGAATTCA TCAAAAAGGC 
TACGGAGTGG TGGGTGTTTC CAGTAAATAC GGAAAACGAT CTTGGGCTAG ATATCCCATA 
TTTTGGGGAT TGAGGAAAAT CGGTAAACTC CCAAATCCCG ACCCAGCAGA TAATGGTACC 
AATTTTAAAG ACCTTGACGC GAATTCCATG AATGAGTTTG AAAACATCAA AGCAAAACAT 
AAGCGTTCAG CTCAAGAATG GGCAAATCTC AATATTGATC CTGAGGCAGA TCTTTTGATA 
TTTGTGGGTC GATGGACGTT GCAAAAGGGC ATTGATTTAA TTGCAGACAT CACTCCTACC 
CTATTAGAAA ATTTCAATTC CCAAATTGTT GTGGTTGGCC CTGTCATTGA TCTTTATGGA 
AAATTTGCTG CTGAAAAGTT CACGGCTTTA ATGAAGAAGT ATCCTGGTCG AATATACTCT 
AGGCCTCTCT TTACTCAACT TCCTTCTTAC ATATTCAGTG GCGCAGATTT TGCTTTGATA 
CCCTCAAGAG ATGAACCTTT TGGTTTGGTG GCTGTTGAAT TTGGTAGGAA AGGAACATTG 
GGTATTGGTG CCAAAGTTGG AGGCCTTGGA CAGATGCCGG GATGGTGGTA TACCATCGAA 
TCTAACACAA CTGCACATTT GCTCTGCCAG TTTGAAGAAG CCTGTCGTCA AGCCTTAACA 
TCTTCAAAGT CTGTGCGCAC CAAACTTCGA GCTATTTCTA CTATTCAGCG GTTTCCTGTT 
TCTGAATGGG TCTCAAAGCT TGATACTCAT GTGAGAAATT GTATCAAGTT CAGCCATAAA 
CAAAACTTAG AAGAAGACTT TATTCATGAA CCTGTAATAG ATGTTGATGA ATTTGCGATA 
TCGTCATCAA AAGATATTGA TGCAGATGAA GATTTAGAAA TTATCGGAAG CTCAGATAAC 
AAAGCGATTG ATTCAAACGG TGAAGGATTT TTAATTGAAA AAGATAATAT TGGAACGGGA 
AGCTACTCAA ATCAACAATC ATTTGACTTT AAAAGTAGCG AAAGTGATTC ATTTCCCCAG 
AAAAGCCCTT CGGTCGAAAG TTTTTCTATA ATTGATAACG ATAATCCTTT CCACGAAGGA 
CAAAATTCTT CTACTGGTTA CAAGGACATT GTGCAAGGGC TTTTGGCTGA AAATGGTGTT 
TCAGAAGCTG GAGTTGACGT TATGACTTCT ATAGTTTCAT CCACTATTCC AATTGTATCA 
AACCATCAAA CTGAAGGTTC TCAAATGTTT AATGAAATCT CATCTGTGTC GTCTATTCAT 
GTATACCATG ATGAATCACA ACCGCCAGTT GAAATGCCAG CTGAAAGTGA CACTCCCTTA 
CAAAATAAAT TATACCATCC AGGGATGTGG TCGTCTTCTG ACATTAGAAT ACCAAATAAT 
AGCTCTCAAT TGAGTATTGA TTCAGTTCGA TCCGGTATGC GACCATTTTC ACTTTCCAAG 
GTACCTCATC AATTTGATGA TGAGGAGGGA AAAGCGCTTC AAATATTTCG AGAGAAATTA 
AAAGATCTTA ATTGCAAAAA TTCTATGAAC GAAATGTGTA TTGAAAATTT TCTCATGAAA 
TGTACTAGAA AATATTTTGA TGAAGTCCGA AAATTACGAC TTGGTACTTT AAAACCTGAA 
AATCTTCAAT TTGTTAAGGA CCCATCTAGT TTGGCTTTGG AAACTAACCT TTTACCAGCA 
TCCGACACAA TTGAGGAAAA GAACGACGTA GGTAATAAAC AGGTGAATTC GCATATATTA 
GATCCAGGGT TTCTCAAAGA AGAAGAATGT GTATATGAAT TTGAGCAACT TCATGGACTT 
CGAAAGGCTT TACAAGTTGA AATTTATGGA TGGCCATTAT ATACAATTTT GCTAGCTATT 
GGACAAGTGT TAGCTGCTAC CTCTTTTCAA CTGAATTTAT TTTCAGATAC GCCTGATCAG 
CCAGAATACC AATCCTATAT CGTGTGTAGT GTTTATATCA GTGCATCTCT ATTCTGGTAT 
ATTTTGCATT CTCTCGTACC CAATATTTAC GCACTTTCAA CCCCATTCAT AATTTACGCT 
ACTGCGTTTG CTTTAGCTGG CCTTACAACG TTTTCAAGCC TCGGTGATTC ACGTCTTTGG 
ATTTCACGAG TTGCAACCTG GATTTATTCA ATTGCCTCTG GGAGTCAAGC TTTGTTTTTT 
TCTTTAAATT TTGGTAATGA AGGAAGATAT GATATCTTGT ATTGGATTGT GAGAGCTTGT 
TTTATTCAAG GATCGCAACA AGTATGGTCG GCAGCACTTT GGTATTGGGG TTCTTACTCA 
ACGGATAAAC CAAATAGAAT GGGAGTTTCG GGAAAATTAA ACCCACAGCC ATGGATGGCA 
GCCATTACAT GGCCAATAGC TTTGGTTTTA CTCGCGATCG CTTTTGTTGT GTATCGAGGT 
TTGCCTAATT TTTACAGACA ATGTCCTTCA AAAATACCAG CTTTCTATCG TTCCTTGTTT 
CGCCGTCGGT TGATAATGTG GTTTTTTATT AGCGTTTTTC TTCAAAATTA CTGGATGTCA 
TCGGTGTATG GTCGATCGTG GGCCTTTATG TGGTCTGCAC ATAATGTACA TAAATGGGCT 
ATGTTTTTAC TAGTCATTTT GTTTTATGTT GCTATATGGA TTGCTTTGGT GGCATTACTT 
GCTTCTTTGT CCAGATACCA TTCTTGGATC CTACCTATTT TGGGATTAGG ATTTGGAGCT 
CCTCGCTGGT TACAAACATT ATGGGGCACG TCAAATATTG GCATATACGT ACCGTTTTTA 
GGAAAAGGTG CACCCTATTT ATCAAGAATG CTGTGGCTCT ATCTTGGCTT ATTGGATACA 
GTTCAAACTG TCGGTATTGG AATTATATTA TTGCAGACAC TAACAAGAGA GCACATTACT 
GTTGTTTTAA TAACCGGGCA AATTGTCGGT GCTATTGCAA GTATGGTAGG TAAAGCCAGC 
TCCCCATCAA AATTTGGACC AGGGGATGTC TTTATAGATT TCACACATTG GAGCGTTAAG 
GATGGTCCCC AGATCCTTGC AAGCATTCCA TTTTGGGTAT GCTTGATTTG TCAATTATCT 
GTTATTATCG GTTACTTCCT GTTCTTTCGC CGTGAAAATT TAAGTCGACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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mok11 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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