did2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following did2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the did2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100143 NM_001022483.2
Latest version!
Schizosaccharomyces pombe putative ESCRT III complex subunit Did2 (did2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100143
    Clone ID Related Accession (Same CDS sequence) NM_001022483.2
    Accession Version NM_001022483.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative ESCRT III complex subunit Did2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022483.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAGCAATT TAGAGGCATC TCTGTTTCAA TTAAAGTTTG CTGCAAAGTC GCTGAATAAA 
    CAATCACTTA AAGCTGCGAA AGAGGAGCGT GCTGAACGGG AAAAAGTGAA AAAGGCTATC
    ACAAAAGGCA ACTCAGAAAT TGCCCGCATA TATGCATCGA ATGCAATTCG GAAGCAGCAA
    GAATCCCTTA ATTTGCTCAA ACTTTCTTCA AGAATAGATG CGGTTTCAAG TCGTTTACAG
    ACTGCTGTAA CTATGCGTGC TGTGTCGGGA AATATGGCAG GTGTCGTTCG GGGAATGGAC
    AGAGCTATGA AAACAATGAA TTTGGAGATG ATTTCACAAG TCATGGATAA ATTTGAAGCG
    CAATTCGATG ATGTTAATGT TCAAACGGGT TATATGAATA AGGCTATGGG ATCAGTGACT
    GCTGTCGATA CACCCCAAGA AGATGTAGAT TTATTAATGC AAACGGTAGC TGATGAAGCT
    GGTTTGGAAT TTAACCAGAA TATGAACAAT AATCTTTCGG TTCCTGCTGC CAGTGTTCCT
    ACTCCCGCCG CTCCTGTAGA AGATGACAAC TTACAAGAAC GTTTACGTGC TTTAAGGTCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596562.2
    CDS45..647
    Translation

    Target ORF information:

    RefSeq Version NM_001022483.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative ESCRT III complex subunit Did2 (did2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001022483.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAGCAATT TAGAGGCATC TCTGTTTCAA TTAAAGTTTG CTGCAAAGTC GCTGAATAAA 
    CAATCACTTA AAGCTGCGAA AGAGGAGCGT GCTGAACGGG AAAAAGTGAA AAAGGCTATC
    ACAAAAGGCA ACTCAGAAAT TGCCCGCATA TATGCATCGA ATGCAATTCG GAAGCAGCAA
    GAATCCCTTA ATTTGCTCAA ACTTTCTTCA AGAATAGATG CGGTTTCAAG TCGTTTACAG
    ACTGCTGTAA CTATGCGTGC TGTGTCGGGA AATATGGCAG GTGTCGTTCG GGGAATGGAC
    AGAGCTATGA AAACAATGAA TTTGGAGATG ATTTCACAAG TCATGGATAA ATTTGAAGCG
    CAATTCGATG ATGTTAATGT TCAAACGGGT TATATGAATA AGGCTATGGG ATCAGTGACT
    GCTGTCGATA CACCCCAAGA AGATGTAGAT TTATTAATGC AAACGGTAGC TGATGAAGCT
    GGTTTGGAAT TTAACCAGAA TATGAACAAT AATCTTTCGG TTCCTGCTGC CAGTGTTCCT
    ACTCCCGCCG CTCCTGTAGA AGATGACAAC TTACAAGAAC GTTTACGTGC TTTAAGGTCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L