nup186 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	nup186
Entrez Gene ID	2538754
Full Name	nucleoporin Nup186
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001023390.2	NP_588399.1	nucleoporin Nup186

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nup186 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nup186 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99650	NM_001023390.2 Latest version!	Schizosaccharomyces pombe nucleoporin Nup186 (nup186), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nup186 ( NM_001023390.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588399.1 Schizosaccharomyces pombe nucleoporin Nup186 (nup186), mRNA.

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CloneID	OSc99650
Clone ID Related Accession (Same CDS sequence)	NM_001023390.2
Accession Version	NM_001023390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	nucleoporin Nup186
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023390.1.

ATGGATGTCT GGGAATCTCA GCACTTTTCC GCTCTTTTAT ACTTATTGCA GTGTATCGAA 
AGTAATCCCA GCAATCCAAA TGTTTCTTCT CGTTTGTTAA TACAATGTCT TGAATCCTAC 
TCAAAGGATT TTCTAAAATT TTTGGCTTTA GATCCAGCTA ATGCTAATTC TCGCAAAAAA 
TTGGAATCCG GTGAAGTTGA ATTAGGCGGT GTAATCAACA AAGTCAATGA GCAGTTCATC 
CAGTTGTCTC TTACTCTCTC TACTCAGTTA AATTTGGATG AAATTCAATG CGCAAGTTTA 
CTACAACGTG GCATTGAAGC GTCTCAAAAT CTTGATCGAA CTCCCGTTCA AGCTGCGTTG 
TATTTCTTTT TTCTAGCTCG AGAACAACTC TTGGAATGTT TAGAATCATT AACAAGGGTT 
GTAGGCTTAA AAGACTTGGA GTCGGACATT TCTACAGCCC TGAAGTCTTA TCTTCAGAGC 
TTATGCGAAA ATGGCAATAA TTTGGTGAAG ACTTGCATTG ACACAATACC TATTTTAGAT 
AATAAAGTCT CAGAGATATT GAAAAGTGAG GCGGGCGGCC AAATACTTGG AGTATCTGAA 
GTTGTTGATT TTCAAGAATT TATTAGACTC TCTCATGAGG CACATGTCGC GGAGTTGGAA 
ACTATTTCTG TTATACTTTA CCAACTCGCA AAAGTAGACT TGTTTCAAAA TTCTCATTTT 
GAAAGCCTTT TGGTCATGTT AAGGAAGTAC GACTCCCCAA ATAAAAATGC AGTTTTGATT 
TTACCCACTT TATATGCTTT CATAGACAAG GTATTAGAAG TTGAATATCT TCCTGATCAG 
AAAGTACAGT TGAGATCTAA TTCTGTCGAG ATTCTTCAGA AAATCCACCA GGCAATTATT 
CAATCACCTT CCCAAGATTG GCGTTCATCT CAATTCAAAA ATATTCTCGG CATATGGTGG 
GTTACCCGTC TTAATGCGAC ATGCAAACAG ATTGAAAAAG TTCCTTCTTT TATAGATTAC 
GAGACCACGA TAAAAAACGC TGCAAATGAA ATCATTCAAA ATGGTGTTTT TTCTGATATG 
ATTACACTGC TTGTATATCC CTTTCGTCAA TCAGAAACTG AAGGTATGGA ATGGGCGTTT 
GCGTTCAAAT CCCGTTCTAG AATCACCGTT AACTGGTCTC TTATTCGACC GTTTATTGCA 
TCCATTATTT TTTCGGAGCT TCGTTCTTTT GCACAAGCAT TTGTCAGTTA TATGCCCGAT 
ATACTAAAAA CCCTTCGCTT GTTGGAAGAA GACCGCTACT TAACAAACAC TACTTACCCT 
ACTTCTATCC CTGGTGAACA AATTGAAGAA TACTTTCCAT TTGAAGAATT CTATTATTTA 
CTATCATCTA TTTACACCTA TAATGTTTCC TGGATATCAG ATTTTTGGGA CGATATTGAA 
AGTGATATGT ATGGATTTCT CACTTGGTCA ATGGGATCTC AGATTCCTGG AATCATTACT 
GCATTTACAC TTTTACTTGC CTCATTATGC AAGAATACCA CTAGTGCATC TAAAATATAC 
GAATTATTCT CAGAGCCCAT ACCCGAAGTT GGACATTTAG AAAGTTTAAT GATAACTAGT 
CCAAGTTGGT CTTATATCTT TAATGTTTTT CGTTACTACA TATCGCATTT AAAGCCAGTA 
CAAACGGTTG TCACTTCATC TGGTCTCGCA CGAGTACATA CAGATCCAAG TGAAATTGAC 
ACTGACTCTG CATTGATTTT ACAAGCCTAT ATCTTGTTAT TCTCTTCTGT GGTGAGGCAA 
GATGCTCAAA TTGCAAGCAC ATTTTGTGAA AATCAAGATT TAAATCCTAT TGCTACTCTG 
TTTGAACTTT TAGAATGTCG GCTACCTGAT TCAGTTAGAA TTTGTATCGT TCGAGCTTTG 
GAATCCTTAG CCCATTTGTC GACTGGTTCA TTCAACAACG CTTTATGGAC AGCTCTTGAT 
AATTGGTTTG TAAGCTCGGT ATTGTTTGAT GTTGATGGTG GGTTAGCACC TATGTCAATT 
CCTGCCATTT CTAAGCGATC ATTAACCAAG CCAGTTACTT CCTGTGGCCC ATTATTAAAT 
AATATACGAA GACTTACTGT GAATTTGGAA ATGAAGATTT CATTTGTTAA CCTCCTTACG 
TCGTTGACTC GTAATAAGAG TGAACTCAAT GTTAATTTAA CGTTTCCTGA GAATTTGGGT 
GCTTCCTATC GTACACCAGG AGTTCAACCT TATGTTGATT ATGTCGTGGA AACTTTTGTG 
GCTTCTTCTA CTCAGTGGAG GTTAATGCGT GATGTTGGAT CCATCAGGTT ACAGTATGCA 
TGTTTACAAT ATATGCTCGC CGTCCTTGAT GGTCTCAACA TTGATTTGTT ATTATACTCT 
CGAATTCTTA GCAGTAAGGT CAGGGATAAT CTTCAAAATA ACAATTTGCA TGTTTATTTG 
ACTAGACATC CTGCAATATC TCTTCTCGAA GCTCTTTACA CCGAAAGTGT ATATTCCGGT 
TTGTTTGACT TGGTTGAATA CGGCTTTGAC CAATTGGAAG ATGTTTCTGT TCCCAAGACG 
ATCGTTATAA CGGTTTCGGC GTCTCTTTGT ATATTACGCA ATGTTCTATC TCTCCAGCGT 
GTTTTGTTCA AGAACGTGGT TCCTTATATA GCTGAGCTAG GAATTAGCAA GTATATTTTG 
GATTTGACTA TATCTAGAAG AGCTTATAAA GAGGTGTTTA TGACTCGAAT TTCTTCTATC 
GTACATCTTG CGTTATTAGT TGGTTCTCGC CATAAATGTT TTCTTCGCTC AGCCATAGAG 
ATATTGAGTT ATCTGGTAGA TGCAGAAGGT TTCATGAACA AAAGAAATCC TGATGAAAAT 
AAGCTGTTGT GCTCAATCAT TAGAACAGCT AACGAAAGCA AACGTATAAT TTTTGGCTTT 
ATTCGCACGT TTGAAAGCCA ATTTTTAACT TTGTTATCTA CCAATGATGA ATCTTCCTTG 
ATTCTAAAAT TGCTTTTGAA TAATCTTAAA CAGAGTGGTG GAGTTTATTC ACTTGCTCTA 
TTGATCTTAG GATTTGATAT ATCCTCGACA AATGTGATTA CATTGCGAGA TCAGCCTGGC 
TACGTTGGCT CTCGGGTTTC TCTCATGAAT TCCCTGCTAG ACTTTATTGA AGGAAGGACA 
ATTGTTAATG GTATTTGGGA AACTCCTGTT ATTATGGTTC AAGCTTTAGA GATTGTAGCC 
TTTTTATGTT CTTGTCCTCT GACTTCTGAA GTTACGTTAT CTGTTATTCG AGCTAGACCT 
GGGTTGCTTG TTAAAATGGT AGATGGTGAG CCTATACTTA CCCAGCAAGT CATTCAGAAT 
CTTGGCGGAT TTTCTTCGGA AGAAGTAAGT ATGGTATCCA GATGCATCCG TTCTCGTACA 
CAGATAATGA ACATGCTGGC GACCGAAATA CATTATGCTG CCTCTGTAGG TCAAAACAAA 
TACTTAAATG AATATGTGGC ATCTCTTATA AGAACAAATG AAAAAACACA TAGCACAGAG 
CTTTCTCAAA AAGAGTCTGG TTTCAAAATC TTAGAATTTA TGGATATATT ACGAATTGAC 
CCTCAAAGCA TAACATTTGA GCTACCCAAT ATCCCTGGTT TTAATTTGAA TATGTTTATT 
ACACGATTCG ATCGTGGTCA CAGTGATTTT CAATTTGACA CTGAACGTGT ACTCAAGATT 
TACCGTTTGT TTTTTGAAGC AGAAATGACT AGTCTGGGGG GTTCGGCTGA GGAAAAATAC 
TCTTGGTTAG AGCAACAGAA TTCAAAGTTG AAAGAATTGG CTCAGAGACT AAACACCTTT 
AATGCACACG TTGTACTTTT GCAAGATATC CATGGATGTC TAACTGCATG GGCAAGACTA 
ACTGGTCTTT TGGTTGACGA TTGTAATGAG GTGATATCGG ATGTACATTT TGATGATTTC 
ATCTGGGAGG TGCTTCGACT TGTGCTGCCT GGTGTTACTG TGCATAATCT CGGTACTCAA 
GGTACAGTAT CTGTGACGAG TTCAGTCATT GAGACAATTT TACCACATGC TCTAAGGAGA 
ATCGGAGCTT TAAAACCTGA AGAGCTTGGC AAATCTACTT ATTTTATGGA GGGTATACAT 
GATGTTATTG TAGGCTTATT GAAAGGTATT CAGTGCCAAC AATCCGATGA AAGCATCCGT 
GAAAATCTTT ATGGATCATT GTTAAGTATT TTGACCGTAT TTCAAAAACA TACATCTGCT 
AATGGAGGCG ATAAGGTTGA TCCAGTTTTC AAGGATGCAT TCCAAAAACT AATTACACCT 
AACTTACTTA CCAGTCAATT TTTTGATGTT TTGACTAAAG ACGCCTTATA TACCAATGGC 
TCCTGCTGGG AACTTTCAGT CATTATATTA AATTTCTTGC ATCATGTGTC CCCTGATATA 
TCTACTCATC TATACAAGTA TTATCTACGT CGGAACTTTG TAAGTTCCTT TATCGATGCT 
TTTTCACGAG CCTTTTCAGA GTTGTTGTCC TCTAATAAAG ATGTATTGGA AGTTCTGAGT 
GGTCTTGAAG CCGGCCAATG TCTTCTAATT ACATTTGCAC AAAACAAACT TACGGTCCAC 
TCTGTTCTTA CTTTTGACTA CATTAAATTG ATGGTTCAGA TGTTATTACA ATTGTGCAAA 
CAAGGTGGAA TTCAGTACCT TAAGCCTCCT GTCCAGCGTT TAATGATTCA GTTACTGCAA 
ATTTTTATCT TGGTTTTAAT GAGAGTTACG CTGAAAGAGA TTTCGAACAA GGATAAGCTC 
CTTCTCCAGT CTATTTTCTT ACTTTCGCGT AAATTGCAAG ATTCTGTTCA GACTGGTGCT 
GATCAAGCTC TCAGTCCGGA AATGAATACG GTCAAAAAAT ATGTTGAAAC CATAAGTCGG 
CTCTTGGATC TTTATAGAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_588399.1
CDS	396..5339
Translation	MDVWESQHFSALLYLLQCIESNPSNPNVSSRLLIQCLESYSKDFLKFLALDPANANSRKKLESGEVELGGVINKVNEQFIQLSLTLSTQLNLDEIQCASLLQRGIEASQNLDRTPVQAALYFFFLAREQLLECLESLTRVVGLKDLESDISTALKSYLQSLCENGNNLVKTCIDTIPILDNKVSEILKSEAGGQILGVSEVVDFQEFIRLSHEAHVAELETISVILYQLAKVDLFQNSHFESLLVMLRKYDSPNKNAVLILPTLYAFIDKVLEVEYLPDQKVQLRSNSVEILQKIHQAIIQSPSQDWRSSQFKNILGIWWVTRLNATCKQIEKVPSFIDYETTIKNAANEIIQNGVFSDMITLLVYPFRQSETEGMEWAFAFKSRSRITVNWSLIRPFIASIIFSELRSFAQAFVSYMPDILKTLRLLEEDRYLTNTTYPTSIPGEQIEEYFPFEEFYYLLSSIYTYNVSWISDFWDDIESDMYGFLTWSMGSQIPGIITAFTLLLASLCKNTTSASKIYELFSEPIPEVGHLESLMITSPSWSYIFNVFRYYISHLKPVQTVVTSSGLARVHTDPSEIDTDSALILQAYILLFSSVVRQDAQIASTFCENQDLNPIATLFELLECRLPDSVRICIVRALESLAHLSTGSFNNALWTALDNWFVSSVLFDVDGGLAPMSIPAISKRSLTKPVTSCGPLLNNIRRLTVNLEMKISFVNLLTSLTRNKSELNVNLTFPENLGASYRTPGVQPYVDYVVETFVASSTQWRLMRDVGSIRLQYACLQYMLAVLDGLNIDLLLYSRILSSKVRDNLQNNNLHVYLTRHPAISLLEALYTESVYSGLFDLVEYGFDQLEDVSVPKTIVITVSASLCILRNVLSLQRVLFKNVVPYIAELGISKYILDLTISRRAYKEVFMTRISSIVHLALLVGSRHKCFLRSAIEILSYLVDAEGFMNKRNPDENKLLCSIIRTANESKRIIFGFIRTFESQFLTLLSTNDESSLILKLLLNNLKQSGGVYSLALLILGFDISSTNVITLRDQPGYVGSRVSLMNSLLDFIEGRTIVNGIWETPVIMVQALEIVAFLCSCPLTSEVTLSVIRARPGLLVKMVDGEPILTQQVIQNLGGFSSEEVSMVSRCIRSRTQIMNMLATEIHYAASVGQNKYLNEYVASLIRTNEKTHSTELSQKESGFKILEFMDILRIDPQSITFELPNIPGFNLNMFITRFDRGHSDFQFDTERVLKIYRLFFEAEMTSLGGSAEEKYSWLEQQNSKLKELAQRLNTFNAHVVLLQDIHGCLTAWARLTGLLVDDCNEVISDVHFDDFIWEVLRLVLPGVTVHNLGTQGTVSVTSSVIETILPHALRRIGALKPEELGKSTYFMEGIHDVIVGLLKGIQCQQSDESIRENLYGSLLSILTVFQKHTSANGGDKVDPVFKDAFQKLITPNLLTSQFFDVLTKDALYTNGSCWELSVIILNFLHHVSPDISTHLYKYYLRRNFVSSFIDAFSRAFSELLSSNKDVLEVLSGLEAGQCLLITFAQNKLTVHSVLTFDYIKLMVQMLLQLCKQGGIQYLKPPVQRLMIQLLQIFILVLMRVTLKEISNKDKLLLQSIFLLSRKLQDSVQTGADQALSPEMNTVKKYVETISRLLDLYRD

Target ORF information:

RefSeq Version	NM_001023390.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe nucleoporin Nup186 (nup186), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023390.2

ORF Insert Sequence:

ATGGATGTCT GGGAATCTCA GCACTTTTCC GCTCTTTTAT ACTTATTGCA GTGTATCGAA 
AGTAATCCCA GCAATCCAAA TGTTTCTTCT CGTTTGTTAA TACAATGTCT TGAATCCTAC 
TCAAAGGATT TTCTAAAATT TTTGGCTTTA GATCCAGCTA ATGCTAATTC TCGCAAAAAA 
TTGGAATCCG GTGAAGTTGA ATTAGGCGGT GTAATCAACA AAGTCAATGA GCAGTTCATC 
CAGTTGTCTC TTACTCTCTC TACTCAGTTA AATTTGGATG AAATTCAATG CGCAAGTTTA 
CTACAACGTG GCATTGAAGC GTCTCAAAAT CTTGATCGAA CTCCCGTTCA AGCTGCGTTG 
TATTTCTTTT TTCTAGCTCG AGAACAACTC TTGGAATGTT TAGAATCATT AACAAGGGTT 
GTAGGCTTAA AAGACTTGGA GTCGGACATT TCTACAGCCC TGAAGTCTTA TCTTCAGAGC 
TTATGCGAAA ATGGCAATAA TTTGGTGAAG ACTTGCATTG ACACAATACC TATTTTAGAT 
AATAAAGTCT CAGAGATATT GAAAAGTGAG GCGGGCGGCC AAATACTTGG AGTATCTGAA 
GTTGTTGATT TTCAAGAATT TATTAGACTC TCTCATGAGG CACATGTCGC GGAGTTGGAA 
ACTATTTCTG TTATACTTTA CCAACTCGCA AAAGTAGACT TGTTTCAAAA TTCTCATTTT 
GAAAGCCTTT TGGTCATGTT AAGGAAGTAC GACTCCCCAA ATAAAAATGC AGTTTTGATT 
TTACCCACTT TATATGCTTT CATAGACAAG GTATTAGAAG TTGAATATCT TCCTGATCAG 
AAAGTACAGT TGAGATCTAA TTCTGTCGAG ATTCTTCAGA AAATCCACCA GGCAATTATT 
CAATCACCTT CCCAAGATTG GCGTTCATCT CAATTCAAAA ATATTCTCGG CATATGGTGG 
GTTACCCGTC TTAATGCGAC ATGCAAACAG ATTGAAAAAG TTCCTTCTTT TATAGATTAC 
GAGACCACGA TAAAAAACGC TGCAAATGAA ATCATTCAAA ATGGTGTTTT TTCTGATATG 
ATTACACTGC TTGTATATCC CTTTCGTCAA TCAGAAACTG AAGGTATGGA ATGGGCGTTT 
GCGTTCAAAT CCCGTTCTAG AATCACCGTT AACTGGTCTC TTATTCGACC GTTTATTGCA 
TCCATTATTT TTTCGGAGCT TCGTTCTTTT GCACAAGCAT TTGTCAGTTA TATGCCCGAT 
ATACTAAAAA CCCTTCGCTT GTTGGAAGAA GACCGCTACT TAACAAACAC TACTTACCCT 
ACTTCTATCC CTGGTGAACA AATTGAAGAA TACTTTCCAT TTGAAGAATT CTATTATTTA 
CTATCATCTA TTTACACCTA TAATGTTTCC TGGATATCAG ATTTTTGGGA CGATATTGAA 
AGTGATATGT ATGGATTTCT CACTTGGTCA ATGGGATCTC AGATTCCTGG AATCATTACT 
GCATTTACAC TTTTACTTGC CTCATTATGC AAGAATACCA CTAGTGCATC TAAAATATAC 
GAATTATTCT CAGAGCCCAT ACCCGAAGTT GGACATTTAG AAAGTTTAAT GATAACTAGT 
CCAAGTTGGT CTTATATCTT TAATGTTTTT CGTTACTACA TATCGCATTT AAAGCCAGTA 
CAAACGGTTG TCACTTCATC TGGTCTCGCA CGAGTACATA CAGATCCAAG TGAAATTGAC 
ACTGACTCTG CATTGATTTT ACAAGCCTAT ATCTTGTTAT TCTCTTCTGT GGTGAGGCAA 
GATGCTCAAA TTGCAAGCAC ATTTTGTGAA AATCAAGATT TAAATCCTAT TGCTACTCTG 
TTTGAACTTT TAGAATGTCG GCTACCTGAT TCAGTTAGAA TTTGTATCGT TCGAGCTTTG 
GAATCCTTAG CCCATTTGTC GACTGGTTCA TTCAACAACG CTTTATGGAC AGCTCTTGAT 
AATTGGTTTG TAAGCTCGGT ATTGTTTGAT GTTGATGGTG GGTTAGCACC TATGTCAATT 
CCTGCCATTT CTAAGCGATC ATTAACCAAG CCAGTTACTT CCTGTGGCCC ATTATTAAAT 
AATATACGAA GACTTACTGT GAATTTGGAA ATGAAGATTT CATTTGTTAA CCTCCTTACG 
TCGTTGACTC GTAATAAGAG TGAACTCAAT GTTAATTTAA CGTTTCCTGA GAATTTGGGT 
GCTTCCTATC GTACACCAGG AGTTCAACCT TATGTTGATT ATGTCGTGGA AACTTTTGTG 
GCTTCTTCTA CTCAGTGGAG GTTAATGCGT GATGTTGGAT CCATCAGGTT ACAGTATGCA 
TGTTTACAAT ATATGCTCGC CGTCCTTGAT GGTCTCAACA TTGATTTGTT ATTATACTCT 
CGAATTCTTA GCAGTAAGGT CAGGGATAAT CTTCAAAATA ACAATTTGCA TGTTTATTTG 
ACTAGACATC CTGCAATATC TCTTCTCGAA GCTCTTTACA CCGAAAGTGT ATATTCCGGT 
TTGTTTGACT TGGTTGAATA CGGCTTTGAC CAATTGGAAG ATGTTTCTGT TCCCAAGACG 
ATCGTTATAA CGGTTTCGGC GTCTCTTTGT ATATTACGCA ATGTTCTATC TCTCCAGCGT 
GTTTTGTTCA AGAACGTGGT TCCTTATATA GCTGAGCTAG GAATTAGCAA GTATATTTTG 
GATTTGACTA TATCTAGAAG AGCTTATAAA GAGGTGTTTA TGACTCGAAT TTCTTCTATC 
GTACATCTTG CGTTATTAGT TGGTTCTCGC CATAAATGTT TTCTTCGCTC AGCCATAGAG 
ATATTGAGTT ATCTGGTAGA TGCAGAAGGT TTCATGAACA AAAGAAATCC TGATGAAAAT 
AAGCTGTTGT GCTCAATCAT TAGAACAGCT AACGAAAGCA AACGTATAAT TTTTGGCTTT 
ATTCGCACGT TTGAAAGCCA ATTTTTAACT TTGTTATCTA CCAATGATGA ATCTTCCTTG 
ATTCTAAAAT TGCTTTTGAA TAATCTTAAA CAGAGTGGTG GAGTTTATTC ACTTGCTCTA 
TTGATCTTAG GATTTGATAT ATCCTCGACA AATGTGATTA CATTGCGAGA TCAGCCTGGC 
TACGTTGGCT CTCGGGTTTC TCTCATGAAT TCCCTGCTAG ACTTTATTGA AGGAAGGACA 
ATTGTTAATG GTATTTGGGA AACTCCTGTT ATTATGGTTC AAGCTTTAGA GATTGTAGCC 
TTTTTATGTT CTTGTCCTCT GACTTCTGAA GTTACGTTAT CTGTTATTCG AGCTAGACCT 
GGGTTGCTTG TTAAAATGGT AGATGGTGAG CCTATACTTA CCCAGCAAGT CATTCAGAAT 
CTTGGCGGAT TTTCTTCGGA AGAAGTAAGT ATGGTATCCA GATGCATCCG TTCTCGTACA 
CAGATAATGA ACATGCTGGC GACCGAAATA CATTATGCTG CCTCTGTAGG TCAAAACAAA 
TACTTAAATG AATATGTGGC ATCTCTTATA AGAACAAATG AAAAAACACA TAGCACAGAG 
CTTTCTCAAA AAGAGTCTGG TTTCAAAATC TTAGAATTTA TGGATATATT ACGAATTGAC 
CCTCAAAGCA TAACATTTGA GCTACCCAAT ATCCCTGGTT TTAATTTGAA TATGTTTATT 
ACACGATTCG ATCGTGGTCA CAGTGATTTT CAATTTGACA CTGAACGTGT ACTCAAGATT 
TACCGTTTGT TTTTTGAAGC AGAAATGACT AGTCTGGGGG GTTCGGCTGA GGAAAAATAC 
TCTTGGTTAG AGCAACAGAA TTCAAAGTTG AAAGAATTGG CTCAGAGACT AAACACCTTT 
AATGCACACG TTGTACTTTT GCAAGATATC CATGGATGTC TAACTGCATG GGCAAGACTA 
ACTGGTCTTT TGGTTGACGA TTGTAATGAG GTGATATCGG ATGTACATTT TGATGATTTC 
ATCTGGGAGG TGCTTCGACT TGTGCTGCCT GGTGTTACTG TGCATAATCT CGGTACTCAA 
GGTACAGTAT CTGTGACGAG TTCAGTCATT GAGACAATTT TACCACATGC TCTAAGGAGA 
ATCGGAGCTT TAAAACCTGA AGAGCTTGGC AAATCTACTT ATTTTATGGA GGGTATACAT 
GATGTTATTG TAGGCTTATT GAAAGGTATT CAGTGCCAAC AATCCGATGA AAGCATCCGT 
GAAAATCTTT ATGGATCATT GTTAAGTATT TTGACCGTAT TTCAAAAACA TACATCTGCT 
AATGGAGGCG ATAAGGTTGA TCCAGTTTTC AAGGATGCAT TCCAAAAACT AATTACACCT 
AACTTACTTA CCAGTCAATT TTTTGATGTT TTGACTAAAG ACGCCTTATA TACCAATGGC 
TCCTGCTGGG AACTTTCAGT CATTATATTA AATTTCTTGC ATCATGTGTC CCCTGATATA 
TCTACTCATC TATACAAGTA TTATCTACGT CGGAACTTTG TAAGTTCCTT TATCGATGCT 
TTTTCACGAG CCTTTTCAGA GTTGTTGTCC TCTAATAAAG ATGTATTGGA AGTTCTGAGT 
GGTCTTGAAG CCGGCCAATG TCTTCTAATT ACATTTGCAC AAAACAAACT TACGGTCCAC 
TCTGTTCTTA CTTTTGACTA CATTAAATTG ATGGTTCAGA TGTTATTACA ATTGTGCAAA 
CAAGGTGGAA TTCAGTACCT TAAGCCTCCT GTCCAGCGTT TAATGATTCA GTTACTGCAA 
ATTTTTATCT TGGTTTTAAT GAGAGTTACG CTGAAAGAGA TTTCGAACAA GGATAAGCTC 
CTTCTCCAGT CTATTTTCTT ACTTTCGCGT AAATTGCAAG ATTCTGTTCA GACTGGTGCT 
GATCAAGCTC TCAGTCCGGA AATGAATACG GTCAAAAAAT ATGTTGAAAC CATAAGTCGG 
CTCTTGGATC TTTATAGAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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nup186 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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