lemd3 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	lemd3
Entrez Gene ID	111225014
Full Name	LEM domain containing 3
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022749752.1	XP_022605473.1	inner nuclear membrane protein Man1

Pathways from BioSystems
Gene Ontology

The following lemd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lemd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe219035	XM_022749752.1 Latest version!	Seriola dumerili LEM domain containing 3 (lemd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lemd3 ( XM_022749752.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022605473.1 Seriola dumerili LEM domain containing 3 (lemd3), mRNA.

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CloneID	OSe219035
Clone ID Related Accession (Same CDS sequence)	XM_022749752.1
Accession Version	XM_022749752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	inner nuclear membrane protein Man1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174393.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCAA TGCAGTTAAC GGATGAGGAG CTTTTCTCCG AATTAAAGCG TTTCGGTTTC 
ACTCCAGGCC CGGTTACCGA GAATACCCGT CCGGTGTATT TAAAGAAATT GAAGAAGCTT 
CGCGAAGAGC AACAGCAGCG GGGCTCCAGA TCGGGTAAAA CCCGTAGCAG CGGGGCCATC 
CACAGCACCA CTGGCGGCGG CAACACGGCG GGATCCAGGC CAGCCAGCCA TGACGTCACG 
CACCTGAGCT CTAACAGGAG ACCGGGCCGG AAGTCCTCCG TCCTCGGCTT CAGCTCCGAC 
GAGTCTGACG CTGAAACTCC ACTGAAAAGA AAGGGCCTCA ATCACAGCGG CAGGGCAGAC 
CGAAGCTCCG GTTTTCAACA ACAACCGAAG ATAAGGCCTG TTACAACACC GACTGTGGCT 
ATCAAGAGTC AGTATGGCGC TGGGAGTACG CTGAATAGCA GCAGTTCTTC CCCGGGTCCG 
GACGGACAGA GAGGTGTCTC GCTGGGCTGG GGAGTTCGTG CCAGATCCAG CCCTGAGGCG 
AAAGGGAGGG ATTACGACGA ATCGGGAGAA GAGGACGATT ATGAGGGGGA AACGGAGAAG 
AACTCTCGCT CTGTAAATGG CTGCGGGGCG TCTCACTTGA TCACTAGCAA GTTAGCCGGG 
GATTACTCAG ACTCCGACGA GGAGGAGGTT GGGGGCCTTG GCGGCGTAGA CCGACAGCGG 
GATAGGTTAG AGCCCAGACG GAGCCACCCG AAAGCGGCGT TCCCTTCCCA CGGAGTCGGC 
CGAGGGTCTC AGACGGGAGG GGCAGCCAGA GAGGTTAACT CGCCTGGGAG TCTAAACATG 
GTGGGAAGAC GGAGGGAGGA GGGGGAGGAA GACGAGGTGA AGAGAAGGGA CTCGGACCCA 
TCGGGAGGCT TGCGGAGCCA CAACTTTCCC AGGAAGTCCA TCTACGTCTC CCTCTCCGAG 
AACCATGGAA GAGAGGCCGG CAAAAACAAC CACGTCGACA GCGAGGACGG ATCCTTCAGA 
AGCAGCAGCT CTAATAGGTT CAGCATCGGG CTGAGACCGC GCTTCTCCAA CTACAGCAGC 
CTGTCCCAGC CTTACAGGGG CAACCACTCC AACCACACCG CTCCCAACCA CTCATACAGC 
CAGACAGCGC TGAAGCAAAA GCTGTCTGTC CCGGAGGATG AGCTGCTCCA GCAATTCAAA 
AGGGAAGAAG TGGCCTCCTC TGGTAGCTTC AGCGCTCACT ACCTGTCCAT GTTCCTGCTC 
ACGGCAGCCT GCCTCTTCTT TCTGCTGCTA GGCCTCATGT ACCTTAGGAT GAGGGGCTCT 
GGATCCTCAG AGGTGGATGG AGTCATTAAG AGCCACCCTT TTGGCGGCGA GTTTGACTCT 
TCTTATGACA AGGCAGAGAA GGACCTGATC CTGAAGCTGC TGCTCAATCT ACACGACCAC 
CTGGCCCACA TCGCTGGCCA GCACGACTGT GGAGACCAGA AGTGTCCAAA CAGGAGTCTG 
TCCATGGACG AGGCCTCTGC GTATTTACGG GCCCAGAATC AGGAGTTTGA AGGCTTCATT 
CATTTGTCTC TGGAGTGGAT CATCCGGGCA GGACAGGACG TGGGCATAAG GCTGACTGGG 
CAGGTAGCTG ATAACCCGGT GACCGATGTG TCTGAGATCT CTCGGCTGGA GTCCACGCAC 
CCCAAGATGC CCTTCACATG CCGCTTCCGT CGAGCCTTCG TCACCGTCAT CAGCAGAGTC 
TTCGTCATCG CAGTCGTGGT GGGCTGTGTG TGGAGCGTGG TCTGGTACAT GAAGTATCGC 
TGGAGGAGGG AGGAGGAGGA GACCAGGCAG ATGTATGACA TGGTGGAGAG GATCATCGAC 
GTGTTGAGGA GCCACACTGA GGCTTGCCAA GAGAACCAGG ACCTACAGCC CTACATGCCC 
ATCCCCCATG TCAGAGACTC CCTGGTTCTG CCTCAGGATC GGAAGAAGAT GAAGAAGATC 
TGGGACCGGG CTGTGAGCTT CCTCTCAGCC GACGAGTCCA GGATTCGAAC AGAGAATCAG 
AGGATCGGCG GAGCGGACTT CCTGGTCTGG AGGTGGATCC AGCCTTCTTT CAGTTGCGAC 
AAGACCTCCT TAATGCCCTC CAAAGTTTGG CAGGGAAAAG CGTTCCCTCT GGACCGGAGG 
AACTCGCCTC CCAACAGCCT GACTCCATGT CTGAAGATCA GGAACATGTT TGACCCGGTC 
ATGGAGGTTG GGGAGAACTG GGATCTAGCC ATCCAGCAGG CGATCCTGGA GAAGTGCAGT 
GACAACGACG GCATCGTCCA CATCACTGTT GACAAGAACT CACGAGAGGG CTGCGTCTAT 
GTCAAGTGTC TCTCTGCAGA GCACTCAGGG AAAGCCTTCA AGGCACTTCA TGGCTCCTGG 
TTTGATGGTA AGCTGGTGAC AGTAAAGTAT CTGCGTTTGG ACCGATACCA CCAGCGCTTC 
CCGCAGGCAC AGGGCTGCAA CACGCCCCTG AAGGCCTCCA GCCTCCTCCA CCCGAACACC 
ACAAGCACCG CGGACACTAC GCCCAGCCTG CAGCACCACG GCTCAGCCAA CTCTTCAGGC 
TTCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022605473.1
CDS	459..3047
Translation	MASMQLTDEELFSELKRFGFTPGPVTENTRPVYLKKLKKLREEQQQRGSRSGKTRSSGAIHSTTGGGNTAGSRPASHDVTHLSSNRRPGRKSSVLGFSSDESDAETPLKRKGLNHSGRADRSSGFQQQPKIRPVTTPTVAIKSQYGAGSTLNSSSSSPGPDGQRGVSLGWGVRARSSPEAKGRDYDESGEEDDYEGETEKNSRSVNGCGASHLITSKLAGDYSDSDEEEVGGLGGVDRQRDRLEPRRSHPKAAFPSHGVGRGSQTGGAAREVNSPGSLNMVGRRREEGEEDEVKRRDSDPSGGLRSHNFPRKSIYVSLSENHGREAGKNNHVDSEDGSFRSSSSNRFSIGLRPRFSNYSSLSQPYRGNHSNHTAPNHSYSQTALKQKLSVPEDELLQQFKREEVASSGSFSAHYLSMFLLTAACLFFLLLGLMYLRMRGSGSSEVDGVIKSHPFGGEFDSSYDKAEKDLILKLLLNLHDHLAHIAGQHDCGDQKCPNRSLSMDEASAYLRAQNQEFEGFIHLSLEWIIRAGQDVGIRLTGQVADNPVTDVSEISRLESTHPKMPFTCRFRRAFVTVISRVFVIAVVVGCVWSVVWYMKYRWRREEEETRQMYDMVERIIDVLRSHTEACQENQDLQPYMPIPHVRDSLVLPQDRKKMKKIWDRAVSFLSADESRIRTENQRIGGADFLVWRWIQPSFSCDKTSLMPSKVWQGKAFPLDRRNSPPNSLTPCLKIRNMFDPVMEVGENWDLAIQQAILEKCSDNDGIVHITVDKNSREGCVYVKCLSAEHSGKAFKALHGSWFDGKLVTVKYLRLDRYHQRFPQAQGCNTPLKASSLLHPNTTSTADTTPSLQHHGSANSSGFS

Target ORF information:

RefSeq Version	XM_022749752.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili LEM domain containing 3 (lemd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022749752.1

ORF Insert Sequence:

ATGGCGTCAA TGCAGTTAAC GGATGAGGAG CTTTTCTCCG AATTAAAGCG TTTCGGTTTC 
ACTCCAGGCC CGGTTACCGA GAATACCCGT CCGGTGTATT TAAAGAAATT GAAGAAGCTT 
CGCGAAGAGC AACAGCAGCG GGGCTCCAGA TCGGGTAAAA CCCGTAGCAG CGGGGCCATC 
CACAGCACCA CTGGCGGCGG CAACACGGCG GGATCCAGGC CAGCCAGCCA TGACGTCACG 
CACCTGAGCT CTAACAGGAG ACCGGGCCGG AAGTCCTCCG TCCTCGGCTT CAGCTCCGAC 
GAGTCTGACG CTGAAACTCC ACTGAAAAGA AAGGGCCTCA ATCACAGCGG CAGGGCAGAC 
CGAAGCTCCG GTTTTCAACA ACAACCGAAG ATAAGGCCTG TTACAACACC GACTGTGGCT 
ATCAAGAGTC AGTATGGCGC TGGGAGTACG CTGAATAGCA GCAGTTCTTC CCCGGGTCCG 
GACGGACAGA GAGGTGTCTC GCTGGGCTGG GGAGTTCGTG CCAGATCCAG CCCTGAGGCG 
AAAGGGAGGG ATTACGACGA ATCGGGAGAA GAGGACGATT ATGAGGGGGA AACGGAGAAG 
AACTCTCGCT CTGTAAATGG CTGCGGGGCG TCTCACTTGA TCACTAGCAA GTTAGCCGGG 
GATTACTCAG ACTCCGACGA GGAGGAGGTT GGGGGCCTTG GCGGCGTAGA CCGACAGCGG 
GATAGGTTAG AGCCCAGACG GAGCCACCCG AAAGCGGCGT TCCCTTCCCA CGGAGTCGGC 
CGAGGGTCTC AGACGGGAGG GGCAGCCAGA GAGGTTAACT CGCCTGGGAG TCTAAACATG 
GTGGGAAGAC GGAGGGAGGA GGGGGAGGAA GACGAGGTGA AGAGAAGGGA CTCGGACCCA 
TCGGGAGGCT TGCGGAGCCA CAACTTTCCC AGGAAGTCCA TCTACGTCTC CCTCTCCGAG 
AACCATGGAA GAGAGGCCGG CAAAAACAAC CACGTCGACA GCGAGGACGG ATCCTTCAGA 
AGCAGCAGCT CTAATAGGTT CAGCATCGGG CTGAGACCGC GCTTCTCCAA CTACAGCAGC 
CTGTCCCAGC CTTACAGGGG CAACCACTCC AACCACACCG CTCCCAACCA CTCATACAGC 
CAGACAGCGC TGAAGCAAAA GCTGTCTGTC CCGGAGGATG AGCTGCTCCA GCAATTCAAA 
AGGGAAGAAG TGGCCTCCTC TGGTAGCTTC AGCGCTCACT ACCTGTCCAT GTTCCTGCTC 
ACGGCAGCCT GCCTCTTCTT TCTGCTGCTA GGCCTCATGT ACCTTAGGAT GAGGGGCTCT 
GGATCCTCAG AGGTGGATGG AGTCATTAAG AGCCACCCTT TTGGCGGCGA GTTTGACTCT 
TCTTATGACA AGGCAGAGAA GGACCTGATC CTGAAGCTGC TGCTCAATCT ACACGACCAC 
CTGGCCCACA TCGCTGGCCA GCACGACTGT GGAGACCAGA AGTGTCCAAA CAGGAGTCTG 
TCCATGGACG AGGCCTCTGC GTATTTACGG GCCCAGAATC AGGAGTTTGA AGGCTTCATT 
CATTTGTCTC TGGAGTGGAT CATCCGGGCA GGACAGGACG TGGGCATAAG GCTGACTGGG 
CAGGTAGCTG ATAACCCGGT GACCGATGTG TCTGAGATCT CTCGGCTGGA GTCCACGCAC 
CCCAAGATGC CCTTCACATG CCGCTTCCGT CGAGCCTTCG TCACCGTCAT CAGCAGAGTC 
TTCGTCATCG CAGTCGTGGT GGGCTGTGTG TGGAGCGTGG TCTGGTACAT GAAGTATCGC 
TGGAGGAGGG AGGAGGAGGA GACCAGGCAG ATGTATGACA TGGTGGAGAG GATCATCGAC 
GTGTTGAGGA GCCACACTGA GGCTTGCCAA GAGAACCAGG ACCTACAGCC CTACATGCCC 
ATCCCCCATG TCAGAGACTC CCTGGTTCTG CCTCAGGATC GGAAGAAGAT GAAGAAGATC 
TGGGACCGGG CTGTGAGCTT CCTCTCAGCC GACGAGTCCA GGATTCGAAC AGAGAATCAG 
AGGATCGGCG GAGCGGACTT CCTGGTCTGG AGGTGGATCC AGCCTTCTTT CAGTTGCGAC 
AAGACCTCCT TAATGCCCTC CAAAGTTTGG CAGGGAAAAG CGTTCCCTCT GGACCGGAGG 
AACTCGCCTC CCAACAGCCT GACTCCATGT CTGAAGATCA GGAACATGTT TGACCCGGTC 
ATGGAGGTTG GGGAGAACTG GGATCTAGCC ATCCAGCAGG CGATCCTGGA GAAGTGCAGT 
GACAACGACG GCATCGTCCA CATCACTGTT GACAAGAACT CACGAGAGGG CTGCGTCTAT 
GTCAAGTGTC TCTCTGCAGA GCACTCAGGG AAAGCCTTCA AGGCACTTCA TGGCTCCTGG 
TTTGATGGTA AGCTGGTGAC AGTAAAGTAT CTGCGTTTGG ACCGATACCA CCAGCGCTTC 
CCGCAGGCAC AGGGCTGCAA CACGCCCCTG AAGGCCTCCA GCCTCCTCCA CCCGAACACC 
ACAAGCACCG CGGACACTAC GCCCAGCCTG CAGCACCACG GCTCAGCCAA CTCTTCAGGC 
TTCTCATGA