LOC103853626 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103853626
Entrez Gene ID	103853626
Full Name	beta-amylase 8
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009130533.2	XP_009128781.1	beta-amylase 8

Pathways from BioSystems
Gene Ontology

The following LOC103853626 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103853626 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr133980	XM_009130533.2 Latest version!	Brassica rapa beta-amylase 8 (LOC103853626), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103853626 ( XM_009130533.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009128781.1 Brassica rapa beta-amylase 8 (LOC103853626), mRNA.

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CloneID	OBr133980
Clone ID Related Accession (Same CDS sequence)	XM_009130533.2
Accession Version	XM_009130533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	beta-amylase 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024796.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009130533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTCGC TAAACACCAC CACCACCGGT TCACAAGATC CGATCGACCC AAACCGAAAC 
CCCGACCCGG ATCAGTACCC CCACCGAAAC CCCAGCCATC CCCAGTCTCG ACGCCCCCGC 
GGGTTCGCGG CAACTGCTGC GGCGGCGTCG ATGGGTCAAG CCGACAGCGA TGTGAACAAC 
GGAAACATAA CCGGAGAGAC GAGCAGCGGC GGCGGAGGAG GGAAGGGGAA GAGAGAGAGG 
GAGAAGGAGA AAGAGAGAAC GAAGCTTAGG GAAAGACACA GACGAGCCAT CACTAGTAGA 
ATGCTCGCCG GGTTGAGACA GTACGGTAAC TTCCCGTTAC CGGCGCGTGC GGATATGAAC 
GATGTAATCG CTGCTTTGGC GCGTGAAGCT GGATGGAGCG TTGACGCCGA CGGTACTACT 
TACCGCCAGT CACATCAAAC CAACCATGTG GTTCAGTTTC CGGCGAGATC TATTGATAAT 
CCTCTTTCTT CAAGTACTTT GAAGAACTGT GCTAAGGCTG CACTTGAATG TCAGCAGCAG 
CATCCTTCTC TTAGAATCGA CGACAACCTT TCTCCTGTCT CTCTTGATTC CGTTGTTATC 
GCCGAGAGTG ATCATCCCGG GAATGGAAGG TATACAGGTG CTAGCCCTAT CCCTTCAGTG 
GGATGCGTCG ATGCCAATCA GCTTATACAA GATGTTCATT CCACAGAGCG CCGTAATGAC 
TACACAGAGA ACTTTTATGT CCCAGTGTAC GCCATGCTTC CTGTTGGCGT TATAGACAAC 
TTCGGCCAGT TGGTTGATCC TCAAGGTGTA AGGCAAGAAC TAAGCTACAT GAAGTCATTA 
AACGTCGATG GAGTTGTAAT AGATTGTTGG TGGGGGATAG TTGAAGGCTG GAACCCTCAG 
AAATATGTGT GGTCTGGCTA CAGAGAGCTC TTTAACCTTA TCAGAGACTT CAAACTCAAA 
CTACAGGTGG TGATGGCATT TCATGAATAT GGAGGGAATG CATCTGGAAA CGTTATGATC 
TCGTTGCCAC AGTGGGTTTT GGAGATTGGG AAAGACAATC CAGACATATT CTTCACTGAT 
CGTGAAGGAA GAAGGAGCTT CGAGTGTTTG AATTGGAGCA TTGACAAGGA ACGGGTATTG 
CATGGACGAA CTGGTATAGA GGTATATTTT GATTTTATGA GAAGCTTCCG GTCAGAGTTC 
GATGACCTGT TTGTGGAGGG TCTAATTGCT GGAATAGAGA TTGGTCTTGG AGCTTCTGGT 
GAACTAAAGT ATCCATCTTT CCCTGAAAGG ATGGGTTGGA TCTATCCTGG TATCGGTGAG 
TTTCAGTGTT ATGACAAGTA TTCACAAACG AGTCTGCTAA AGGAAGCAAA GTCGAGGGGG 
TTCGCGTTCT GGGGTAAAGG ACCAGAGAAT GCTGGTCAAT ACAATTCACA ACCTCATGAA 
ACTGGTTTCT TCCAGGAGAG AGGTGAATAT GACAGCTACT ACGGTCGCTT CTTCTTGAAC 
TGGTACTCTC AACTACTGAT AGGACATGCA GAGAATGTTC TTTCTCTTGC CAATCTCGCC 
TTTGAGGAGA CAAAGATTAT TGTCAAGATA CCGGCGATTT ACTGGTCATA CAAGATAGCT 
AGTCACGCTG CTGAGCTAAC TGCAGGATAC TATAACCCTT CAAACCGTGA CGGGTACTCT 
CCTGTTTTCG AAACTCTGAA GAAATATTCA GTGACGGTTA AGTTTGTGTG CCCTGGGCCA 
CAAATGTCTT CTAATGAGCA TGAAGAAGCT CTAGCTGATC CAGAAGGTTT GAGTTGGCAG 
GTGGTCAATG CAGCTTGGGA TAAGGGACTT CTTATTGGGG GAGAGAATGC GATTACTTGT 
TTTGATAGAG GAGGTTGTAT GAGATTAATC GAAATGGCTA AACCAAGAAA CCATCCAGAT 
AGCAATCACT TCTCCTTCTT CACTTACCGC CAACCTTCTC CTCTAGTTCA AGGATCCACT 
TGCTTTGCAG ATTTAGACTA CTTCATAAAA CGCATGCACG GTGATTTACA GAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009128781.1
CDS	72..2108
Translation	MHSLNTTTTGSQDPIDPNRNPDPDQYPHRNPSHPQSRRPRGFAATAAAASMGQADSDVNNGNITGETSSGGGGGKGKREREKEKERTKLRERHRRAITSRMLAGLRQYGNFPLPARADMNDVIAALAREAGWSVDADGTTYRQSHQTNHVVQFPARSIDNPLSSSTLKNCAKAALECQQQHPSLRIDDNLSPVSLDSVVIAESDHPGNGRYTGASPIPSVGCVDANQLIQDVHSTERRNDYTENFYVPVYAMLPVGVIDNFGQLVDPQGVRQELSYMKSLNVDGVVIDCWWGIVEGWNPQKYVWSGYRELFNLIRDFKLKLQVVMAFHEYGGNASGNVMISLPQWVLEIGKDNPDIFFTDREGRRSFECLNWSIDKERVLHGRTGIEVYFDFMRSFRSEFDDLFVEGLIAGIEIGLGASGELKYPSFPERMGWIYPGIGEFQCYDKYSQTSLLKEAKSRGFAFWGKGPENAGQYNSQPHETGFFQERGEYDSYYGRFFLNWYSQLLIGHAENVLSLANLAFEETKIIVKIPAIYWSYKIASHAAELTAGYYNPSNRDGYSPVFETLKKYSVTVKFVCPGPQMSSNEHEEALADPEGLSWQVVNAAWDKGLLIGGENAITCFDRGGCMRLIEMAKPRNHPDSNHFSFFTYRQPSPLVQGSTCFADLDYFIKRMHGDLQR

Target ORF information:

RefSeq Version	XM_009130533.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa beta-amylase 8 (LOC103853626), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009130533.2

ORF Insert Sequence:

ATGCACTCGC TAAACACCAC CACCACCGGT TCACAAGATC CGATCGACCC AAACCGAAAC 
CCCGACCCGG ATCAGTACCC CCACCGAAAC CCCAGCCATC CCCAGTCTCG ACGCCCCCGC 
GGGTTCGCGG CAACTGCTGC GGCGGCGTCG ATGGGTCAAG CCGACAGCGA TGTGAACAAC 
GGAAACATAA CCGGAGAGAC GAGCAGCGGC GGCGGAGGAG GGAAGGGGAA GAGAGAGAGG 
GAGAAGGAGA AAGAGAGAAC GAAGCTTAGG GAAAGACACA GACGAGCCAT CACTAGTAGA 
ATGCTCGCCG GGTTGAGACA GTACGGTAAC TTCCCGTTAC CGGCGCGTGC GGATATGAAC 
GATGTAATCG CTGCTTTGGC GCGTGAAGCT GGATGGAGCG TTGACGCCGA CGGTACTACT 
TACCGCCAGT CACATCAAAC CAACCATGTG GTTCAGTTTC CGGCGAGATC TATTGATAAT 
CCTCTTTCTT CAAGTACTTT GAAGAACTGT GCTAAGGCTG CACTTGAATG TCAGCAGCAG 
CATCCTTCTC TTAGAATCGA CGACAACCTT TCTCCTGTCT CTCTTGATTC CGTTGTTATC 
GCCGAGAGTG ATCATCCCGG GAATGGAAGG TATACAGGTG CTAGCCCTAT CCCTTCAGTG 
GGATGCGTCG ATGCCAATCA GCTTATACAA GATGTTCATT CCACAGAGCG CCGTAATGAC 
TACACAGAGA ACTTTTATGT CCCAGTGTAC GCCATGCTTC CTGTTGGCGT TATAGACAAC 
TTCGGCCAGT TGGTTGATCC TCAAGGTGTA AGGCAAGAAC TAAGCTACAT GAAGTCATTA 
AACGTCGATG GAGTTGTAAT AGATTGTTGG TGGGGGATAG TTGAAGGCTG GAACCCTCAG 
AAATATGTGT GGTCTGGCTA CAGAGAGCTC TTTAACCTTA TCAGAGACTT CAAACTCAAA 
CTACAGGTGG TGATGGCATT TCATGAATAT GGAGGGAATG CATCTGGAAA CGTTATGATC 
TCGTTGCCAC AGTGGGTTTT GGAGATTGGG AAAGACAATC CAGACATATT CTTCACTGAT 
CGTGAAGGAA GAAGGAGCTT CGAGTGTTTG AATTGGAGCA TTGACAAGGA ACGGGTATTG 
CATGGACGAA CTGGTATAGA GGTATATTTT GATTTTATGA GAAGCTTCCG GTCAGAGTTC 
GATGACCTGT TTGTGGAGGG TCTAATTGCT GGAATAGAGA TTGGTCTTGG AGCTTCTGGT 
GAACTAAAGT ATCCATCTTT CCCTGAAAGG ATGGGTTGGA TCTATCCTGG TATCGGTGAG 
TTTCAGTGTT ATGACAAGTA TTCACAAACG AGTCTGCTAA AGGAAGCAAA GTCGAGGGGG 
TTCGCGTTCT GGGGTAAAGG ACCAGAGAAT GCTGGTCAAT ACAATTCACA ACCTCATGAA 
ACTGGTTTCT TCCAGGAGAG AGGTGAATAT GACAGCTACT ACGGTCGCTT CTTCTTGAAC 
TGGTACTCTC AACTACTGAT AGGACATGCA GAGAATGTTC TTTCTCTTGC CAATCTCGCC 
TTTGAGGAGA CAAAGATTAT TGTCAAGATA CCGGCGATTT ACTGGTCATA CAAGATAGCT 
AGTCACGCTG CTGAGCTAAC TGCAGGATAC TATAACCCTT CAAACCGTGA CGGGTACTCT 
CCTGTTTTCG AAACTCTGAA GAAATATTCA GTGACGGTTA AGTTTGTGTG CCCTGGGCCA 
CAAATGTCTT CTAATGAGCA TGAAGAAGCT CTAGCTGATC CAGAAGGTTT GAGTTGGCAG 
GTGGTCAATG CAGCTTGGGA TAAGGGACTT CTTATTGGGG GAGAGAATGC GATTACTTGT 
TTTGATAGAG GAGGTTGTAT GAGATTAATC GAAATGGCTA AACCAAGAAA CCATCCAGAT 
AGCAATCACT TCTCCTTCTT CACTTACCGC CAACCTTCTC CTCTAGTTCA AGGATCCACT 
TGCTTTGCAG ATTTAGACTA CTTCATAAAA CGCATGCACG GTGATTTACA GAGATAA