GCT cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCT
Entrez Gene ID	841978
Full Name	RNA polymerase II transcription mediator
Synonyms	CCT,CENTER CITY,GRAND CENTRAL,MAB2,MACCHI-BOU 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_179478.2	NP_849809.2	RNA polymerase II transcription mediator
NM_001198308.1	NP_001185237.1	RNA polymerase II transcription mediator
NM_001333703.1	NP_001322789.1	RNA polymerase II transcription mediator
NM_001333704.1	NP_001322788.1	RNA polymerase II transcription mediator

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that HSI2 recruits MED13 and HDA6 to suppress directly a subset of seed maturation genes post-germination.
Title: HSI2 Repressor Recruits MED13 and HDA6 to Down-Regulate Seed Maturation Gene Expression Directly During Arabidopsis Early Seedling Growth.

GCT and CCT coordinate vegetative and floral transitions by repressing the repressors miR156 and FLC.
Title: The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions.

MAB2 plays a crucial role in embryo patterning and cotyledon organogenesis. [MAB2] [MACCHI-BOU 2]
Title: MACCHI-BOU 2 is required for early embryo patterning and cotyledon organogenesis in Arabidopsis.

MED13 and MED12 regulate pattern formation during Arabidopsis embryogenesis by transiently repressing a transcriptional program that interferes with this process [MED13]
Title: The MED12-MED13 module of Mediator regulates the timing of embryo patterning in Arabidopsis.

The following GCT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb34475	NM_001333703.1 Latest version!	Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAb34475	NM_001333704.1 Latest version!	Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAb26459	NM_179478.2 Latest version!	Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OAb30062	NM_001198308.1 Latest version!	Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCT ( NM_001333703.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322789.1 Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.

Price & Availability↑

CloneID	OAb34475
Clone ID Related Accession (Same CDS sequence)	NM_001333703.1 , NM_001333704.1
Accession Version	NM_001333703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNA polymerase II transcription mediator
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGTGGACTA ATGTTTTCAG AATTGGTGGT CTACATAATG TTTCTTGGTT TCAGTTTCTT 
CCAAGTGAAA CTGAGCTGAA TCCTGGCTTT GATAGAAGTT CAAGAGCTGA GCAGAATGAA 
GTTGCAACAT ATCTTGTACT TTCCTCTCAT CTACGGTTGC AGAAGGAAGG GTTTCTTACC 
ACTTGGACCA ATTCTTTTGT TGGACCTTGG GATCCATCTC AAGGTTTATA CAACCCTGAT 
GAAAAGATTA AGCTTTGGCT TTTTCTACCT GGGCGTCATT CATCAATATC TGATAAAGCT 
CAGGCTGCTG TGTCAAAGCT TAGAGTTGTT GCGTCAGGAA TTTGGGTAGC ACCTGGGGAC 
TCTGAAGAGA TATCAGTTGC CTTTTCACAG TCTTTACGTA ATTGCATCGA AAGAGCATTA 
TCTGGAATTT CCTACATGAG ATTTGGAGAT GTGTTTTCAA AATTCAGCCC TCAAAGCGAA 
GAATATTTGA GGAGGGGACA GCCTACTGTT GAATTCATCT TTGCTGCTAC CGAAGAGGCA 
GTTTTTGTCC ATGTCATTAT ATCTGCCAAG AATGTCCGCA CACTTTCAAG TGGCGATGCT 
GAAAGAATGT TAAGGAGTTC TTTGAAAAAC TCCAGTTATA GGCTTCCAGT TATTGTTTCT 
CCTCATGGAA TGCGGGGCAG CCTTACTGGA TTCTGTCCCA ATGACCTTGT CAAGCAAGTC 
TACTTCAGTT CTGGAAACTT AAAAACTTCG ACTGGATATG TTGGTCTCCC TTCTCATATT 
GGTCGTGGGT CCCGTCTGAT AAATGGCAAT CATTGCTACG TGGAAGTTAC ACTTGGTTGC 
TGCCAAAATA GAAATGACAA CACAAGTCAA GCTAATTCGA CCTTTGCAGT TAACTTGCCC 
CATAACCAGT GTCCTGAACC TTCAGTTGGG AGTAAAGATC ACCGTAAAGG ACAATCAGAT 
CTTTCATCAG TTTGTGAAAA AAAGTTCATC TATCCAGCTG AGGCAGTGCT TGTACCAATC 
TTACAGTCGG CATTTGCGAA ATTTTCTTTG AAAAGATTTT GGCTTCAAAA CTGGATAGGC 
CCGTCACTAG CAGGCTCATC TTTGTTTATG CACTGGGCTG GTGATTTTGA CTGCCTTGGT 
GCGTCTGAAA ATAAGAGTGA TGGGTTTTAT GAAAAAAATG GTTACAATAG CAGTGGTAGT 
AGCCGTAATA GCAGCATTAG TTCAACAAGT AGCGCTTCCA GCGGGAGCGG CTGGAGGATG 
ACGTCAAGAA CTGGTGATCT TGACGCTGAT GCAGATTCTT TGACGTGTAG GCAATCTGGT 
CTTACTTGTA ATGATGATCG CCTAAAAATG GGTTCCAAGC GGCCGCGAAC AGGGATGGCA 
GAGTCATTCG GTCAAGTAGG CATTGAAAAT GATCAAATTG GTTGGGATTG GGATGCTGAT 
GATGATGATG ATGACAGAGA AGTTGGCATG GATATCAAGG CACTTCTTTC AGAGTTTGGA 
GACTTTGGTG ACTTCTTTGA GAACGATGCT TTACCTTTCG GGGAGCCACC GGGAACTGCA 
GAGTCGCATG TGCTCGTTTT TCCTCCAGAT TCTGCTGATG TAGGTTCCAG TCCCGTGGAT 
ATGATGGATG TATCAGACCA AATCGTTTTG CCTGTTGGGT TTTCCTCTTT TGAGAGCTTT 
AATCCTGTTC CCCCAATCAT TGATGAGTGC CTTATTAAAA GTCAAGAAGT TCTCCACAGC 
AGTATCACTT CAGTTCCTTC AAATCAAATG TCGATCTCTT CGACTGGTGA GTTTGATCAT 
TTACTAAAAG CAGAAGCAAT GATGACCTTT GCTCCTGAGT ATGGAGCTGT AGAAGCACCT 
ATGAGCGAGA TTTCCTCGAC GTTTTTCAAA AGCCCATATC TTCCCAAATC TCATAAAGTG 
GAGAGTTCAA ATTCAAGAAC GAGTAATTAT GTCTATGGAG CCACACCACC GACCACTGAT 
TCTGATGGAG CAGGCGATAT GATTTTGTTC GGATCAAAAT CATGCATTGG AAACAATGCT 
GGCAGAACTC TGTATCATTC GAGGGAGCAT TACACTCAAG TAGAAGGTAG AAAGGGCCGA 
CATGATAAGC TACCTACAGT TATCAGTGAC AACAGTAGTA CAAAGGAGGG TGTCTCTCAA 
TCAATACACT CAAAACATAG TGCTGCGAAT GCTGTTAAGG TCGTACAGGG AAAAAAAACT 
GATGGTATAT CTGCTGTTGT TAGTACCTTA TTATCTTCAA AGACCCTGCT GGCAACAGAC 
GTGGGAAGTG TTATGTTCCA AGCTTTCATG TGTAGGATGC GGCACATAAT CACTTCTTCA 
AAGCACAGTT CACCTGTTAG TCTGACTAGG CTTAGCGGAA ACTTTTTCCT TAACCAGCTG 
TCAAATGAGC CTAGTACTCT GACAGACAAC ATATCTGCAA GGAACGAGAT ATATAAGAAA 
GATATACCTA CCAGAATAGC TGGAGATTTT GACGGAGGAA TGTTGGATAG CCACATGAGT 
GCACCAGTTG GCGTATGGCG GACTGTTTCA GTCCCAAAAA CAGCAAAACC TGCTAGTTCG 
CCAAATATTG AAGCAGGGTC ATCCTTGCCC CATAGTTCAT TTAGCGAAGA CAGCTTACTT 
TCTTATGGTC AAAGGCAACC ACTGCAGGAT CTCCTAGATG GAATAGCGCT ACTTGTACAG 
CAAGCTACCT CTTTTGTTGA TTTAGCTCTG GATTCAGATT GTGGAGATGG ACCTTATGGC 
TGGCTTGCAT TGGAAGAGTT ATGGAGACGG GAATTGTCGT GTGGTCCCTC TGCTGGCCAT 
GCAGGTTGTG GGGGAACTTT GGCTTCCTGC CATTCTCTGG ACATTGCTGG TGTCAAGCTT 
GTTGATCCAC TCTCTGCTGA GGTTTTTCCT TCGTCTGTCA TTACTCTTCT GCAATCTGAC 
ATCAAAACAG CTCTGAAATC TGCATTTGGT CAGTCAGATG GTCCATTATC TGTTACAGAT 
TGGTGCAAGG GCCGAAATCA ATCGGGGGAT GGAGGATCTA TTTCTGAGGG ATTTACTGCT 
GAGTCAGCCC TGAGTGAAGT CTCAAATGCA ATAGATGGTG GGAAAGGAGA TGAGACGGCT 
CAGAGCCAAG ACATATACAG TTCAGAATTA CTCCGACCGA CACTTTTTGT TCTTCCATCA 
CCTGCTATAC TTGTCGGGTA CCAAGATGAC TGGCTTAAGA TATCAACAAA CTCCTTGACA 
CATTGGGAAA AGGCCCCCTT TGAGCCATAT GCTCTGCCAA AAAGTATTAA TTATGCTGTT 
GTATGCCCGG ATATTGATCC TTTAACTTGT GCTGCTACTG ATTTCTTCCA GCAGCTTGGA 
ACTGTTTATG AAACGTGCAG GCTGGGAACA CATTTGCCAC AGAGCCTGGG AAATCAAATG 
GAAAAGGACG TTGGGAGATT ATCCTCATCT GGATTTGTTC TGCTTGATTG CCCTCAATCA 
ATGAAGATTG AAAGCAATAA CACATCGCTT CTGGGATCTC TCAGCGATTA TTTTCTATCT 
TTATCAAATG GGTGGAATGT GAATAGCTAT CTGAAGTCTT TATCAAAAGC GCTGAAAGGT 
CTAAAGCTTG GATCAGGCCT TTACACAAAC CAAAAAGAAG GATCAGGAAG TCCTTGTGTG 
GTAGTGTACA TAGTGTGTCC ATTTCCTGAC CCTTCGGCAG TTTTAAGAAC GATCGTCGAA 
TCGTCTATAG CACTTGGATC AGTAATACAG TCAGATAGAG ACAGGAGATC AATACTCAAC 
AGTCAAGTTG CAAGGGCGTT TAGTAGTTCT ACTGCTGTTG ACGAAGCATC AATATCTCAC 
ATTCCAGTGC TCTCAGGGTT TAGTGTCCCC AAATTAGTTC TACAAGTGGT GTCTGTTGAT 
TCAATTTTCC GGATAACAAG TCCAAGCTTT AATGAGCTTG TCATTCTCAA GGATACTGCT 
TTTTCTGTAT ACAATAAAGC TAGGAGAATT TCACGAGGAA TGCCTAATGA TGCATTTTTC 
TCATCATCTT TACCAAGCAG ATCGTCTTCA GCGTTGACAC CAATGAACTC TATTTCAGGA 
ATCTGGAAAG ACTGCGGTGG TTCTCGAATG ACAGGTTCTA CACATCCAAG AGATGGTGAA 
ATTGATGTTA GTTTGAGAAC GAGTGGTTGG GATACCTCTA CTTCCTGGCA GATACCAAGA 
TCTGGAGGAT TAAGCTGCGA CCCAAACAGA AATGGAGATT TTTACCTTAA TGATGAAATT 
TTCTATTTAT TTGAACCACT TTTCATTCTT TCCGAGCCTG GTTCTGTGGA GCGTGGAGTT 
TCACCTACTT TCACCAGCTT GGGTTCTGAG TCTTCCAAGC CAATACCCGA GGATGGTGGC 
AGAGGTTCTG GACCTGGCAT GAATTCAATG GAAGGTATAA CATCGGGATC AAGCTCCCAG 
GGGGATGTAT CCCAGCTTGA AGGAAAGGCT ATTCCAAGTT TACATTGCTG CTACGGTTGG 
ACAGAGGACT GGAGATGGCT TGTAAGCATC TGGACGGATG CCAGGGGTGA GTTGCTTGAC 
ACACATATAT TTCCCTTTGG TGGAATTAGC AGTAGACAGG ATACAAAAGG GCTGCAGTGT 
CTTTTCGTTC AAGTTCTGCA GCAGGGATGT CAAATACTAC AGGCGTGCTC CTCCCCTGAC 
AATGGATCTT TCAAACCCAG GGATTTTGTC ATTACACGCA TTGGGAATTT CTTCGAGCTT 
GAATACCAAG AGTGGCAAAA GGCAATTTAC TCAGCTGGAG GTCCTGAGAT TAAAAAGTGG 
CCTATTCAAC TGCGACGTTC TGCGCCTTCT GGCATAGCCA CCAACAGCAA CGGATCTTCC 
TTGCAACAGC AGGATCTGAG TCTGATTCAA GAGAGAGCCT CATCAACTAG CACACTCTAC 
AGCTCTCATT CAAAACAATC CACCTTTGTG AAAGGCAGTA TGGGGCAATC CGCTGGAAGA 
AAGCAGATAA TGGGTGGGCA GACCATTTCT GGTACTCCAA GGGGATTGTT TCAGTGGGTT 
CACAGCATCA GTTTTGCTTC TATTTCACTT GACCATTCCC TGCATTTTGT TCTTCCAGCT 
GAGTTGGTAT CCGCTGGTGG TGGCCAGAGC AGTACTGGTA TGAGTTCCGT TAATTACATT 
GAAGGTTTCA CTCCTGTCAA GTCTCTTGGT TCTACCGCTT TCTCTTACAT GATGATACCA 
TCACCCAACA TGCGCTTTCT TCACCCAAGT CCTCTTCAGC TTCCGACATG TTTAACTGCC 
GAGTCACCTC CACTTGCTCA CCTCCTTCAC AGCAAAGGCT ATGCAATCCC CTTGTCTACT 
GGATTTGTTG TTTCAAAAGC TGTGCCTTCC ATGAGAAAAG ACTCAAGGAT AAACGTGAAA 
GAAGAATGGC CATCAGTTCT TTCTGTAAGT CTCATAGACT ATTATGGTGG TTATGACAAC 
GCTCATGACA AAATTCTTCA GGGAATCGTG AAGCAGGGAG GAGGGACCAA AGAAACTAGA 
GATTTTGAGG TTGAAAGCCA TCTTATCCTT GAGTCAATTG CGGCAGAACT CCATGCTCTA 
TCATGGATGA CTGTTAGTCC AGCATATCTG GACAGGCGGA CGGCATTGCC GTTTCACTGT 
GATATGGTTC TGAGATTGAG ACGTCTTCTT CACTTTGCGG ACAAAGAAGT CTCCAGGATA 
CCTGATAAGA CAGGAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322789.1
CDS	262..5982
Translation	MWTNVFRIGGLHNVSWFQFLPSETELNPGFDRSSRAEQNEVATYLVLSSHLRLQKEGFLTTWTNSFVGPWDPSQGLYNPDEKIKLWLFLPGRHSSISDKAQAAVSKLRVVASGIWVAPGDSEEISVAFSQSLRNCIERALSGISYMRFGDVFSKFSPQSEEYLRRGQPTVEFIFAATEEAVFVHVIISAKNVRTLSSGDAERMLRSSLKNSSYRLPVIVSPHGMRGSLTGFCPNDLVKQVYFSSGNLKTSTGYVGLPSHIGRGSRLINGNHCYVEVTLGCCQNRNDNTSQANSTFAVNLPHNQCPEPSVGSKDHRKGQSDLSSVCEKKFIYPAEAVLVPILQSAFAKFSLKRFWLQNWIGPSLAGSSLFMHWAGDFDCLGASENKSDGFYEKNGYNSSGSSRNSSISSTSSASSGSGWRMTSRTGDLDADADSLTCRQSGLTCNDDRLKMGSKRPRTGMAESFGQVGIENDQIGWDWDADDDDDDREVGMDIKALLSEFGDFGDFFENDALPFGEPPGTAESHVLVFPPDSADVGSSPVDMMDVSDQIVLPVGFSSFESFNPVPPIIDECLIKSQEVLHSSITSVPSNQMSISSTGEFDHLLKAEAMMTFAPEYGAVEAPMSEISSTFFKSPYLPKSHKVESSNSRTSNYVYGATPPTTDSDGAGDMILFGSKSCIGNNAGRTLYHSREHYTQVEGRKGRHDKLPTVISDNSSTKEGVSQSIHSKHSAANAVKVVQGKKTDGISAVVSTLLSSKTLLATDVGSVMFQAFMCRMRHIITSSKHSSPVSLTRLSGNFFLNQLSNEPSTLTDNISARNEIYKKDIPTRIAGDFDGGMLDSHMSAPVGVWRTVSVPKTAKPASSPNIEAGSSLPHSSFSEDSLLSYGQRQPLQDLLDGIALLVQQATSFVDLALDSDCGDGPYGWLALEELWRRELSCGPSAGHAGCGGTLASCHSLDIAGVKLVDPLSAEVFPSSVITLLQSDIKTALKSAFGQSDGPLSVTDWCKGRNQSGDGGSISEGFTAESALSEVSNAIDGGKGDETAQSQDIYSSELLRPTLFVLPSPAILVGYQDDWLKISTNSLTHWEKAPFEPYALPKSINYAVVCPDIDPLTCAATDFFQQLGTVYETCRLGTHLPQSLGNQMEKDVGRLSSSGFVLLDCPQSMKIESNNTSLLGSLSDYFLSLSNGWNVNSYLKSLSKALKGLKLGSGLYTNQKEGSGSPCVVVYIVCPFPDPSAVLRTIVESSIALGSVIQSDRDRRSILNSQVARAFSSSTAVDEASISHIPVLSGFSVPKLVLQVVSVDSIFRITSPSFNELVILKDTAFSVYNKARRISRGMPNDAFFSSSLPSRSSSALTPMNSISGIWKDCGGSRMTGSTHPRDGEIDVSLRTSGWDTSTSWQIPRSGGLSCDPNRNGDFYLNDEIFYLFEPLFILSEPGSVERGVSPTFTSLGSESSKPIPEDGGRGSGPGMNSMEGITSGSSSQGDVSQLEGKAIPSLHCCYGWTEDWRWLVSIWTDARGELLDTHIFPFGGISSRQDTKGLQCLFVQVLQQGCQILQACSSPDNGSFKPRDFVITRIGNFFELEYQEWQKAIYSAGGPEIKKWPIQLRRSAPSGIATNSNGSSLQQQDLSLIQERASSTSTLYSSHSKQSTFVKGSMGQSAGRKQIMGGQTISGTPRGLFQWVHSISFASISLDHSLHFVLPAELVSAGGGQSSTGMSSVNYIEGFTPVKSLGSTAFSYMMIPSPNMRFLHPSPLQLPTCLTAESPPLAHLLHSKGYAIPLSTGFVVSKAVPSMRKDSRINVKEEWPSVLSVSLIDYYGGYDNAHDKILQGIVKQGGGTKETRDFEVESHLILESIAAELHALSWMTVSPAYLDRRTALPFHCDMVLRLRRLLHFADKEVSRIPDKTGV

Target ORF information:

RefSeq Version	NM_001333703.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333703.1

ORF Insert Sequence:

ATGTGGACTA ATGTTTTCAG AATTGGTGGT CTACATAATG TTTCTTGGTT TCAGTTTCTT 
CCAAGTGAAA CTGAGCTGAA TCCTGGCTTT GATAGAAGTT CAAGAGCTGA GCAGAATGAA 
GTTGCAACAT ATCTTGTACT TTCCTCTCAT CTACGGTTGC AGAAGGAAGG GTTTCTTACC 
ACTTGGACCA ATTCTTTTGT TGGACCTTGG GATCCATCTC AAGGTTTATA CAACCCTGAT 
GAAAAGATTA AGCTTTGGCT TTTTCTACCT GGGCGTCATT CATCAATATC TGATAAAGCT 
CAGGCTGCTG TGTCAAAGCT TAGAGTTGTT GCGTCAGGAA TTTGGGTAGC ACCTGGGGAC 
TCTGAAGAGA TATCAGTTGC CTTTTCACAG TCTTTACGTA ATTGCATCGA AAGAGCATTA 
TCTGGAATTT CCTACATGAG ATTTGGAGAT GTGTTTTCAA AATTCAGCCC TCAAAGCGAA 
GAATATTTGA GGAGGGGACA GCCTACTGTT GAATTCATCT TTGCTGCTAC CGAAGAGGCA 
GTTTTTGTCC ATGTCATTAT ATCTGCCAAG AATGTCCGCA CACTTTCAAG TGGCGATGCT 
GAAAGAATGT TAAGGAGTTC TTTGAAAAAC TCCAGTTATA GGCTTCCAGT TATTGTTTCT 
CCTCATGGAA TGCGGGGCAG CCTTACTGGA TTCTGTCCCA ATGACCTTGT CAAGCAAGTC 
TACTTCAGTT CTGGAAACTT AAAAACTTCG ACTGGATATG TTGGTCTCCC TTCTCATATT 
GGTCGTGGGT CCCGTCTGAT AAATGGCAAT CATTGCTACG TGGAAGTTAC ACTTGGTTGC 
TGCCAAAATA GAAATGACAA CACAAGTCAA GCTAATTCGA CCTTTGCAGT TAACTTGCCC 
CATAACCAGT GTCCTGAACC TTCAGTTGGG AGTAAAGATC ACCGTAAAGG ACAATCAGAT 
CTTTCATCAG TTTGTGAAAA AAAGTTCATC TATCCAGCTG AGGCAGTGCT TGTACCAATC 
TTACAGTCGG CATTTGCGAA ATTTTCTTTG AAAAGATTTT GGCTTCAAAA CTGGATAGGC 
CCGTCACTAG CAGGCTCATC TTTGTTTATG CACTGGGCTG GTGATTTTGA CTGCCTTGGT 
GCGTCTGAAA ATAAGAGTGA TGGGTTTTAT GAAAAAAATG GTTACAATAG CAGTGGTAGT 
AGCCGTAATA GCAGCATTAG TTCAACAAGT AGCGCTTCCA GCGGGAGCGG CTGGAGGATG 
ACGTCAAGAA CTGGTGATCT TGACGCTGAT GCAGATTCTT TGACGTGTAG GCAATCTGGT 
CTTACTTGTA ATGATGATCG CCTAAAAATG GGTTCCAAGC GGCCGCGAAC AGGGATGGCA 
GAGTCATTCG GTCAAGTAGG CATTGAAAAT GATCAAATTG GTTGGGATTG GGATGCTGAT 
GATGATGATG ATGACAGAGA AGTTGGCATG GATATCAAGG CACTTCTTTC AGAGTTTGGA 
GACTTTGGTG ACTTCTTTGA GAACGATGCT TTACCTTTCG GGGAGCCACC GGGAACTGCA 
GAGTCGCATG TGCTCGTTTT TCCTCCAGAT TCTGCTGATG TAGGTTCCAG TCCCGTGGAT 
ATGATGGATG TATCAGACCA AATCGTTTTG CCTGTTGGGT TTTCCTCTTT TGAGAGCTTT 
AATCCTGTTC CCCCAATCAT TGATGAGTGC CTTATTAAAA GTCAAGAAGT TCTCCACAGC 
AGTATCACTT CAGTTCCTTC AAATCAAATG TCGATCTCTT CGACTGGTGA GTTTGATCAT 
TTACTAAAAG CAGAAGCAAT GATGACCTTT GCTCCTGAGT ATGGAGCTGT AGAAGCACCT 
ATGAGCGAGA TTTCCTCGAC GTTTTTCAAA AGCCCATATC TTCCCAAATC TCATAAAGTG 
GAGAGTTCAA ATTCAAGAAC GAGTAATTAT GTCTATGGAG CCACACCACC GACCACTGAT 
TCTGATGGAG CAGGCGATAT GATTTTGTTC GGATCAAAAT CATGCATTGG AAACAATGCT 
GGCAGAACTC TGTATCATTC GAGGGAGCAT TACACTCAAG TAGAAGGTAG AAAGGGCCGA 
CATGATAAGC TACCTACAGT TATCAGTGAC AACAGTAGTA CAAAGGAGGG TGTCTCTCAA 
TCAATACACT CAAAACATAG TGCTGCGAAT GCTGTTAAGG TCGTACAGGG AAAAAAAACT 
GATGGTATAT CTGCTGTTGT TAGTACCTTA TTATCTTCAA AGACCCTGCT GGCAACAGAC 
GTGGGAAGTG TTATGTTCCA AGCTTTCATG TGTAGGATGC GGCACATAAT CACTTCTTCA 
AAGCACAGTT CACCTGTTAG TCTGACTAGG CTTAGCGGAA ACTTTTTCCT TAACCAGCTG 
TCAAATGAGC CTAGTACTCT GACAGACAAC ATATCTGCAA GGAACGAGAT ATATAAGAAA 
GATATACCTA CCAGAATAGC TGGAGATTTT GACGGAGGAA TGTTGGATAG CCACATGAGT 
GCACCAGTTG GCGTATGGCG GACTGTTTCA GTCCCAAAAA CAGCAAAACC TGCTAGTTCG 
CCAAATATTG AAGCAGGGTC ATCCTTGCCC CATAGTTCAT TTAGCGAAGA CAGCTTACTT 
TCTTATGGTC AAAGGCAACC ACTGCAGGAT CTCCTAGATG GAATAGCGCT ACTTGTACAG 
CAAGCTACCT CTTTTGTTGA TTTAGCTCTG GATTCAGATT GTGGAGATGG ACCTTATGGC 
TGGCTTGCAT TGGAAGAGTT ATGGAGACGG GAATTGTCGT GTGGTCCCTC TGCTGGCCAT 
GCAGGTTGTG GGGGAACTTT GGCTTCCTGC CATTCTCTGG ACATTGCTGG TGTCAAGCTT 
GTTGATCCAC TCTCTGCTGA GGTTTTTCCT TCGTCTGTCA TTACTCTTCT GCAATCTGAC 
ATCAAAACAG CTCTGAAATC TGCATTTGGT CAGTCAGATG GTCCATTATC TGTTACAGAT 
TGGTGCAAGG GCCGAAATCA ATCGGGGGAT GGAGGATCTA TTTCTGAGGG ATTTACTGCT 
GAGTCAGCCC TGAGTGAAGT CTCAAATGCA ATAGATGGTG GGAAAGGAGA TGAGACGGCT 
CAGAGCCAAG ACATATACAG TTCAGAATTA CTCCGACCGA CACTTTTTGT TCTTCCATCA 
CCTGCTATAC TTGTCGGGTA CCAAGATGAC TGGCTTAAGA TATCAACAAA CTCCTTGACA 
CATTGGGAAA AGGCCCCCTT TGAGCCATAT GCTCTGCCAA AAAGTATTAA TTATGCTGTT 
GTATGCCCGG ATATTGATCC TTTAACTTGT GCTGCTACTG ATTTCTTCCA GCAGCTTGGA 
ACTGTTTATG AAACGTGCAG GCTGGGAACA CATTTGCCAC AGAGCCTGGG AAATCAAATG 
GAAAAGGACG TTGGGAGATT ATCCTCATCT GGATTTGTTC TGCTTGATTG CCCTCAATCA 
ATGAAGATTG AAAGCAATAA CACATCGCTT CTGGGATCTC TCAGCGATTA TTTTCTATCT 
TTATCAAATG GGTGGAATGT GAATAGCTAT CTGAAGTCTT TATCAAAAGC GCTGAAAGGT 
CTAAAGCTTG GATCAGGCCT TTACACAAAC CAAAAAGAAG GATCAGGAAG TCCTTGTGTG 
GTAGTGTACA TAGTGTGTCC ATTTCCTGAC CCTTCGGCAG TTTTAAGAAC GATCGTCGAA 
TCGTCTATAG CACTTGGATC AGTAATACAG TCAGATAGAG ACAGGAGATC AATACTCAAC 
AGTCAAGTTG CAAGGGCGTT TAGTAGTTCT ACTGCTGTTG ACGAAGCATC AATATCTCAC 
ATTCCAGTGC TCTCAGGGTT TAGTGTCCCC AAATTAGTTC TACAAGTGGT GTCTGTTGAT 
TCAATTTTCC GGATAACAAG TCCAAGCTTT AATGAGCTTG TCATTCTCAA GGATACTGCT 
TTTTCTGTAT ACAATAAAGC TAGGAGAATT TCACGAGGAA TGCCTAATGA TGCATTTTTC 
TCATCATCTT TACCAAGCAG ATCGTCTTCA GCGTTGACAC CAATGAACTC TATTTCAGGA 
ATCTGGAAAG ACTGCGGTGG TTCTCGAATG ACAGGTTCTA CACATCCAAG AGATGGTGAA 
ATTGATGTTA GTTTGAGAAC GAGTGGTTGG GATACCTCTA CTTCCTGGCA GATACCAAGA 
TCTGGAGGAT TAAGCTGCGA CCCAAACAGA AATGGAGATT TTTACCTTAA TGATGAAATT 
TTCTATTTAT TTGAACCACT TTTCATTCTT TCCGAGCCTG GTTCTGTGGA GCGTGGAGTT 
TCACCTACTT TCACCAGCTT GGGTTCTGAG TCTTCCAAGC CAATACCCGA GGATGGTGGC 
AGAGGTTCTG GACCTGGCAT GAATTCAATG GAAGGTATAA CATCGGGATC AAGCTCCCAG 
GGGGATGTAT CCCAGCTTGA AGGAAAGGCT ATTCCAAGTT TACATTGCTG CTACGGTTGG 
ACAGAGGACT GGAGATGGCT TGTAAGCATC TGGACGGATG CCAGGGGTGA GTTGCTTGAC 
ACACATATAT TTCCCTTTGG TGGAATTAGC AGTAGACAGG ATACAAAAGG GCTGCAGTGT 
CTTTTCGTTC AAGTTCTGCA GCAGGGATGT CAAATACTAC AGGCGTGCTC CTCCCCTGAC 
AATGGATCTT TCAAACCCAG GGATTTTGTC ATTACACGCA TTGGGAATTT CTTCGAGCTT 
GAATACCAAG AGTGGCAAAA GGCAATTTAC TCAGCTGGAG GTCCTGAGAT TAAAAAGTGG 
CCTATTCAAC TGCGACGTTC TGCGCCTTCT GGCATAGCCA CCAACAGCAA CGGATCTTCC 
TTGCAACAGC AGGATCTGAG TCTGATTCAA GAGAGAGCCT CATCAACTAG CACACTCTAC 
AGCTCTCATT CAAAACAATC CACCTTTGTG AAAGGCAGTA TGGGGCAATC CGCTGGAAGA 
AAGCAGATAA TGGGTGGGCA GACCATTTCT GGTACTCCAA GGGGATTGTT TCAGTGGGTT 
CACAGCATCA GTTTTGCTTC TATTTCACTT GACCATTCCC TGCATTTTGT TCTTCCAGCT 
GAGTTGGTAT CCGCTGGTGG TGGCCAGAGC AGTACTGGTA TGAGTTCCGT TAATTACATT 
GAAGGTTTCA CTCCTGTCAA GTCTCTTGGT TCTACCGCTT TCTCTTACAT GATGATACCA 
TCACCCAACA TGCGCTTTCT TCACCCAAGT CCTCTTCAGC TTCCGACATG TTTAACTGCC 
GAGTCACCTC CACTTGCTCA CCTCCTTCAC AGCAAAGGCT ATGCAATCCC CTTGTCTACT 
GGATTTGTTG TTTCAAAAGC TGTGCCTTCC ATGAGAAAAG ACTCAAGGAT AAACGTGAAA 
GAAGAATGGC CATCAGTTCT TTCTGTAAGT CTCATAGACT ATTATGGTGG TTATGACAAC 
GCTCATGACA AAATTCTTCA GGGAATCGTG AAGCAGGGAG GAGGGACCAA AGAAACTAGA 
GATTTTGAGG TTGAAAGCCA TCTTATCCTT GAGTCAATTG CGGCAGAACT CCATGCTCTA 
TCATGGATGA CTGTTAGTCC AGCATATCTG GACAGGCGGA CGGCATTGCC GTTTCACTGT 
GATATGGTTC TGAGATTGAG ACGTCTTCTT CACTTTGCGG ACAAAGAAGT CTCCAGGATA 
CCTGATAAGA CAGGAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCT ( NM_001333704.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322788.1 Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.

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CloneID	OAb34475
Clone ID Related Accession (Same CDS sequence)	NM_001333703.1 , NM_001333704.1
Accession Version	NM_001333704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNA polymerase II transcription mediator
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGTGGACTA ATGTTTTCAG AATTGGTGGT CTACATAATG TTTCTTGGTT TCAGTTTCTT 
CCAAGTGAAA CTGAGCTGAA TCCTGGCTTT GATAGAAGTT CAAGAGCTGA GCAGAATGAA 
GTTGCAACAT ATCTTGTACT TTCCTCTCAT CTACGGTTGC AGAAGGAAGG GTTTCTTACC 
ACTTGGACCA ATTCTTTTGT TGGACCTTGG GATCCATCTC AAGGTTTATA CAACCCTGAT 
GAAAAGATTA AGCTTTGGCT TTTTCTACCT GGGCGTCATT CATCAATATC TGATAAAGCT 
CAGGCTGCTG TGTCAAAGCT TAGAGTTGTT GCGTCAGGAA TTTGGGTAGC ACCTGGGGAC 
TCTGAAGAGA TATCAGTTGC CTTTTCACAG TCTTTACGTA ATTGCATCGA AAGAGCATTA 
TCTGGAATTT CCTACATGAG ATTTGGAGAT GTGTTTTCAA AATTCAGCCC TCAAAGCGAA 
GAATATTTGA GGAGGGGACA GCCTACTGTT GAATTCATCT TTGCTGCTAC CGAAGAGGCA 
GTTTTTGTCC ATGTCATTAT ATCTGCCAAG AATGTCCGCA CACTTTCAAG TGGCGATGCT 
GAAAGAATGT TAAGGAGTTC TTTGAAAAAC TCCAGTTATA GGCTTCCAGT TATTGTTTCT 
CCTCATGGAA TGCGGGGCAG CCTTACTGGA TTCTGTCCCA ATGACCTTGT CAAGCAAGTC 
TACTTCAGTT CTGGAAACTT AAAAACTTCG ACTGGATATG TTGGTCTCCC TTCTCATATT 
GGTCGTGGGT CCCGTCTGAT AAATGGCAAT CATTGCTACG TGGAAGTTAC ACTTGGTTGC 
TGCCAAAATA GAAATGACAA CACAAGTCAA GCTAATTCGA CCTTTGCAGT TAACTTGCCC 
CATAACCAGT GTCCTGAACC TTCAGTTGGG AGTAAAGATC ACCGTAAAGG ACAATCAGAT 
CTTTCATCAG TTTGTGAAAA AAAGTTCATC TATCCAGCTG AGGCAGTGCT TGTACCAATC 
TTACAGTCGG CATTTGCGAA ATTTTCTTTG AAAAGATTTT GGCTTCAAAA CTGGATAGGC 
CCGTCACTAG CAGGCTCATC TTTGTTTATG CACTGGGCTG GTGATTTTGA CTGCCTTGGT 
GCGTCTGAAA ATAAGAGTGA TGGGTTTTAT GAAAAAAATG GTTACAATAG CAGTGGTAGT 
AGCCGTAATA GCAGCATTAG TTCAACAAGT AGCGCTTCCA GCGGGAGCGG CTGGAGGATG 
ACGTCAAGAA CTGGTGATCT TGACGCTGAT GCAGATTCTT TGACGTGTAG GCAATCTGGT 
CTTACTTGTA ATGATGATCG CCTAAAAATG GGTTCCAAGC GGCCGCGAAC AGGGATGGCA 
GAGTCATTCG GTCAAGTAGG CATTGAAAAT GATCAAATTG GTTGGGATTG GGATGCTGAT 
GATGATGATG ATGACAGAGA AGTTGGCATG GATATCAAGG CACTTCTTTC AGAGTTTGGA 
GACTTTGGTG ACTTCTTTGA GAACGATGCT TTACCTTTCG GGGAGCCACC GGGAACTGCA 
GAGTCGCATG TGCTCGTTTT TCCTCCAGAT TCTGCTGATG TAGGTTCCAG TCCCGTGGAT 
ATGATGGATG TATCAGACCA AATCGTTTTG CCTGTTGGGT TTTCCTCTTT TGAGAGCTTT 
AATCCTGTTC CCCCAATCAT TGATGAGTGC CTTATTAAAA GTCAAGAAGT TCTCCACAGC 
AGTATCACTT CAGTTCCTTC AAATCAAATG TCGATCTCTT CGACTGGTGA GTTTGATCAT 
TTACTAAAAG CAGAAGCAAT GATGACCTTT GCTCCTGAGT ATGGAGCTGT AGAAGCACCT 
ATGAGCGAGA TTTCCTCGAC GTTTTTCAAA AGCCCATATC TTCCCAAATC TCATAAAGTG 
GAGAGTTCAA ATTCAAGAAC GAGTAATTAT GTCTATGGAG CCACACCACC GACCACTGAT 
TCTGATGGAG CAGGCGATAT GATTTTGTTC GGATCAAAAT CATGCATTGG AAACAATGCT 
GGCAGAACTC TGTATCATTC GAGGGAGCAT TACACTCAAG TAGAAGGTAG AAAGGGCCGA 
CATGATAAGC TACCTACAGT TATCAGTGAC AACAGTAGTA CAAAGGAGGG TGTCTCTCAA 
TCAATACACT CAAAACATAG TGCTGCGAAT GCTGTTAAGG TCGTACAGGG AAAAAAAACT 
GATGGTATAT CTGCTGTTGT TAGTACCTTA TTATCTTCAA AGACCCTGCT GGCAACAGAC 
GTGGGAAGTG TTATGTTCCA AGCTTTCATG TGTAGGATGC GGCACATAAT CACTTCTTCA 
AAGCACAGTT CACCTGTTAG TCTGACTAGG CTTAGCGGAA ACTTTTTCCT TAACCAGCTG 
TCAAATGAGC CTAGTACTCT GACAGACAAC ATATCTGCAA GGAACGAGAT ATATAAGAAA 
GATATACCTA CCAGAATAGC TGGAGATTTT GACGGAGGAA TGTTGGATAG CCACATGAGT 
GCACCAGTTG GCGTATGGCG GACTGTTTCA GTCCCAAAAA CAGCAAAACC TGCTAGTTCG 
CCAAATATTG AAGCAGGGTC ATCCTTGCCC CATAGTTCAT TTAGCGAAGA CAGCTTACTT 
TCTTATGGTC AAAGGCAACC ACTGCAGGAT CTCCTAGATG GAATAGCGCT ACTTGTACAG 
CAAGCTACCT CTTTTGTTGA TTTAGCTCTG GATTCAGATT GTGGAGATGG ACCTTATGGC 
TGGCTTGCAT TGGAAGAGTT ATGGAGACGG GAATTGTCGT GTGGTCCCTC TGCTGGCCAT 
GCAGGTTGTG GGGGAACTTT GGCTTCCTGC CATTCTCTGG ACATTGCTGG TGTCAAGCTT 
GTTGATCCAC TCTCTGCTGA GGTTTTTCCT TCGTCTGTCA TTACTCTTCT GCAATCTGAC 
ATCAAAACAG CTCTGAAATC TGCATTTGGT CAGTCAGATG GTCCATTATC TGTTACAGAT 
TGGTGCAAGG GCCGAAATCA ATCGGGGGAT GGAGGATCTA TTTCTGAGGG ATTTACTGCT 
GAGTCAGCCC TGAGTGAAGT CTCAAATGCA ATAGATGGTG GGAAAGGAGA TGAGACGGCT 
CAGAGCCAAG ACATATACAG TTCAGAATTA CTCCGACCGA CACTTTTTGT TCTTCCATCA 
CCTGCTATAC TTGTCGGGTA CCAAGATGAC TGGCTTAAGA TATCAACAAA CTCCTTGACA 
CATTGGGAAA AGGCCCCCTT TGAGCCATAT GCTCTGCCAA AAAGTATTAA TTATGCTGTT 
GTATGCCCGG ATATTGATCC TTTAACTTGT GCTGCTACTG ATTTCTTCCA GCAGCTTGGA 
ACTGTTTATG AAACGTGCAG GCTGGGAACA CATTTGCCAC AGAGCCTGGG AAATCAAATG 
GAAAAGGACG TTGGGAGATT ATCCTCATCT GGATTTGTTC TGCTTGATTG CCCTCAATCA 
ATGAAGATTG AAAGCAATAA CACATCGCTT CTGGGATCTC TCAGCGATTA TTTTCTATCT 
TTATCAAATG GGTGGAATGT GAATAGCTAT CTGAAGTCTT TATCAAAAGC GCTGAAAGGT 
CTAAAGCTTG GATCAGGCCT TTACACAAAC CAAAAAGAAG GATCAGGAAG TCCTTGTGTG 
GTAGTGTACA TAGTGTGTCC ATTTCCTGAC CCTTCGGCAG TTTTAAGAAC GATCGTCGAA 
TCGTCTATAG CACTTGGATC AGTAATACAG TCAGATAGAG ACAGGAGATC AATACTCAAC 
AGTCAAGTTG CAAGGGCGTT TAGTAGTTCT ACTGCTGTTG ACGAAGCATC AATATCTCAC 
ATTCCAGTGC TCTCAGGGTT TAGTGTCCCC AAATTAGTTC TACAAGTGGT GTCTGTTGAT 
TCAATTTTCC GGATAACAAG TCCAAGCTTT AATGAGCTTG TCATTCTCAA GGATACTGCT 
TTTTCTGTAT ACAATAAAGC TAGGAGAATT TCACGAGGAA TGCCTAATGA TGCATTTTTC 
TCATCATCTT TACCAAGCAG ATCGTCTTCA GCGTTGACAC CAATGAACTC TATTTCAGGA 
ATCTGGAAAG ACTGCGGTGG TTCTCGAATG ACAGGTTCTA CACATCCAAG AGATGGTGAA 
ATTGATGTTA GTTTGAGAAC GAGTGGTTGG GATACCTCTA CTTCCTGGCA GATACCAAGA 
TCTGGAGGAT TAAGCTGCGA CCCAAACAGA AATGGAGATT TTTACCTTAA TGATGAAATT 
TTCTATTTAT TTGAACCACT TTTCATTCTT TCCGAGCCTG GTTCTGTGGA GCGTGGAGTT 
TCACCTACTT TCACCAGCTT GGGTTCTGAG TCTTCCAAGC CAATACCCGA GGATGGTGGC 
AGAGGTTCTG GACCTGGCAT GAATTCAATG GAAGGTATAA CATCGGGATC AAGCTCCCAG 
GGGGATGTAT CCCAGCTTGA AGGAAAGGCT ATTCCAAGTT TACATTGCTG CTACGGTTGG 
ACAGAGGACT GGAGATGGCT TGTAAGCATC TGGACGGATG CCAGGGGTGA GTTGCTTGAC 
ACACATATAT TTCCCTTTGG TGGAATTAGC AGTAGACAGG ATACAAAAGG GCTGCAGTGT 
CTTTTCGTTC AAGTTCTGCA GCAGGGATGT CAAATACTAC AGGCGTGCTC CTCCCCTGAC 
AATGGATCTT TCAAACCCAG GGATTTTGTC ATTACACGCA TTGGGAATTT CTTCGAGCTT 
GAATACCAAG AGTGGCAAAA GGCAATTTAC TCAGCTGGAG GTCCTGAGAT TAAAAAGTGG 
CCTATTCAAC TGCGACGTTC TGCGCCTTCT GGCATAGCCA CCAACAGCAA CGGATCTTCC 
TTGCAACAGC AGGATCTGAG TCTGATTCAA GAGAGAGCCT CATCAACTAG CACACTCTAC 
AGCTCTCATT CAAAACAATC CACCTTTGTG AAAGGCAGTA TGGGGCAATC CGCTGGAAGA 
AAGCAGATAA TGGGTGGGCA GACCATTTCT GGTACTCCAA GGGGATTGTT TCAGTGGGTT 
CACAGCATCA GTTTTGCTTC TATTTCACTT GACCATTCCC TGCATTTTGT TCTTCCAGCT 
GAGTTGGTAT CCGCTGGTGG TGGCCAGAGC AGTACTGGTA TGAGTTCCGT TAATTACATT 
GAAGGTTTCA CTCCTGTCAA GTCTCTTGGT TCTACCGCTT TCTCTTACAT GATGATACCA 
TCACCCAACA TGCGCTTTCT TCACCCAAGT CCTCTTCAGC TTCCGACATG TTTAACTGCC 
GAGTCACCTC CACTTGCTCA CCTCCTTCAC AGCAAAGGCT ATGCAATCCC CTTGTCTACT 
GGATTTGTTG TTTCAAAAGC TGTGCCTTCC ATGAGAAAAG ACTCAAGGAT AAACGTGAAA 
GAAGAATGGC CATCAGTTCT TTCTGTAAGT CTCATAGACT ATTATGGTGG TTATGACAAC 
GCTCATGACA AAATTCTTCA GGGAATCGTG AAGCAGGGAG GAGGGACCAA AGAAACTAGA 
GATTTTGAGG TTGAAAGCCA TCTTATCCTT GAGTCAATTG CGGCAGAACT CCATGCTCTA 
TCATGGATGA CTGTTAGTCC AGCATATCTG GACAGGCGGA CGGCATTGCC GTTTCACTGT 
GATATGGTTC TGAGATTGAG ACGTCTTCTT CACTTTGCGG ACAAAGAAGT CTCCAGGATA 
CCTGATAAGA CAGGAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322788.1
CDS	255..5975
Translation	MWTNVFRIGGLHNVSWFQFLPSETELNPGFDRSSRAEQNEVATYLVLSSHLRLQKEGFLTTWTNSFVGPWDPSQGLYNPDEKIKLWLFLPGRHSSISDKAQAAVSKLRVVASGIWVAPGDSEEISVAFSQSLRNCIERALSGISYMRFGDVFSKFSPQSEEYLRRGQPTVEFIFAATEEAVFVHVIISAKNVRTLSSGDAERMLRSSLKNSSYRLPVIVSPHGMRGSLTGFCPNDLVKQVYFSSGNLKTSTGYVGLPSHIGRGSRLINGNHCYVEVTLGCCQNRNDNTSQANSTFAVNLPHNQCPEPSVGSKDHRKGQSDLSSVCEKKFIYPAEAVLVPILQSAFAKFSLKRFWLQNWIGPSLAGSSLFMHWAGDFDCLGASENKSDGFYEKNGYNSSGSSRNSSISSTSSASSGSGWRMTSRTGDLDADADSLTCRQSGLTCNDDRLKMGSKRPRTGMAESFGQVGIENDQIGWDWDADDDDDDREVGMDIKALLSEFGDFGDFFENDALPFGEPPGTAESHVLVFPPDSADVGSSPVDMMDVSDQIVLPVGFSSFESFNPVPPIIDECLIKSQEVLHSSITSVPSNQMSISSTGEFDHLLKAEAMMTFAPEYGAVEAPMSEISSTFFKSPYLPKSHKVESSNSRTSNYVYGATPPTTDSDGAGDMILFGSKSCIGNNAGRTLYHSREHYTQVEGRKGRHDKLPTVISDNSSTKEGVSQSIHSKHSAANAVKVVQGKKTDGISAVVSTLLSSKTLLATDVGSVMFQAFMCRMRHIITSSKHSSPVSLTRLSGNFFLNQLSNEPSTLTDNISARNEIYKKDIPTRIAGDFDGGMLDSHMSAPVGVWRTVSVPKTAKPASSPNIEAGSSLPHSSFSEDSLLSYGQRQPLQDLLDGIALLVQQATSFVDLALDSDCGDGPYGWLALEELWRRELSCGPSAGHAGCGGTLASCHSLDIAGVKLVDPLSAEVFPSSVITLLQSDIKTALKSAFGQSDGPLSVTDWCKGRNQSGDGGSISEGFTAESALSEVSNAIDGGKGDETAQSQDIYSSELLRPTLFVLPSPAILVGYQDDWLKISTNSLTHWEKAPFEPYALPKSINYAVVCPDIDPLTCAATDFFQQLGTVYETCRLGTHLPQSLGNQMEKDVGRLSSSGFVLLDCPQSMKIESNNTSLLGSLSDYFLSLSNGWNVNSYLKSLSKALKGLKLGSGLYTNQKEGSGSPCVVVYIVCPFPDPSAVLRTIVESSIALGSVIQSDRDRRSILNSQVARAFSSSTAVDEASISHIPVLSGFSVPKLVLQVVSVDSIFRITSPSFNELVILKDTAFSVYNKARRISRGMPNDAFFSSSLPSRSSSALTPMNSISGIWKDCGGSRMTGSTHPRDGEIDVSLRTSGWDTSTSWQIPRSGGLSCDPNRNGDFYLNDEIFYLFEPLFILSEPGSVERGVSPTFTSLGSESSKPIPEDGGRGSGPGMNSMEGITSGSSSQGDVSQLEGKAIPSLHCCYGWTEDWRWLVSIWTDARGELLDTHIFPFGGISSRQDTKGLQCLFVQVLQQGCQILQACSSPDNGSFKPRDFVITRIGNFFELEYQEWQKAIYSAGGPEIKKWPIQLRRSAPSGIATNSNGSSLQQQDLSLIQERASSTSTLYSSHSKQSTFVKGSMGQSAGRKQIMGGQTISGTPRGLFQWVHSISFASISLDHSLHFVLPAELVSAGGGQSSTGMSSVNYIEGFTPVKSLGSTAFSYMMIPSPNMRFLHPSPLQLPTCLTAESPPLAHLLHSKGYAIPLSTGFVVSKAVPSMRKDSRINVKEEWPSVLSVSLIDYYGGYDNAHDKILQGIVKQGGGTKETRDFEVESHLILESIAAELHALSWMTVSPAYLDRRTALPFHCDMVLRLRRLLHFADKEVSRIPDKTGV

Target ORF information:

RefSeq Version	NM_001333704.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333704.1

ORF Insert Sequence:

ATGTGGACTA ATGTTTTCAG AATTGGTGGT CTACATAATG TTTCTTGGTT TCAGTTTCTT 
CCAAGTGAAA CTGAGCTGAA TCCTGGCTTT GATAGAAGTT CAAGAGCTGA GCAGAATGAA 
GTTGCAACAT ATCTTGTACT TTCCTCTCAT CTACGGTTGC AGAAGGAAGG GTTTCTTACC 
ACTTGGACCA ATTCTTTTGT TGGACCTTGG GATCCATCTC AAGGTTTATA CAACCCTGAT 
GAAAAGATTA AGCTTTGGCT TTTTCTACCT GGGCGTCATT CATCAATATC TGATAAAGCT 
CAGGCTGCTG TGTCAAAGCT TAGAGTTGTT GCGTCAGGAA TTTGGGTAGC ACCTGGGGAC 
TCTGAAGAGA TATCAGTTGC CTTTTCACAG TCTTTACGTA ATTGCATCGA AAGAGCATTA 
TCTGGAATTT CCTACATGAG ATTTGGAGAT GTGTTTTCAA AATTCAGCCC TCAAAGCGAA 
GAATATTTGA GGAGGGGACA GCCTACTGTT GAATTCATCT TTGCTGCTAC CGAAGAGGCA 
GTTTTTGTCC ATGTCATTAT ATCTGCCAAG AATGTCCGCA CACTTTCAAG TGGCGATGCT 
GAAAGAATGT TAAGGAGTTC TTTGAAAAAC TCCAGTTATA GGCTTCCAGT TATTGTTTCT 
CCTCATGGAA TGCGGGGCAG CCTTACTGGA TTCTGTCCCA ATGACCTTGT CAAGCAAGTC 
TACTTCAGTT CTGGAAACTT AAAAACTTCG ACTGGATATG TTGGTCTCCC TTCTCATATT 
GGTCGTGGGT CCCGTCTGAT AAATGGCAAT CATTGCTACG TGGAAGTTAC ACTTGGTTGC 
TGCCAAAATA GAAATGACAA CACAAGTCAA GCTAATTCGA CCTTTGCAGT TAACTTGCCC 
CATAACCAGT GTCCTGAACC TTCAGTTGGG AGTAAAGATC ACCGTAAAGG ACAATCAGAT 
CTTTCATCAG TTTGTGAAAA AAAGTTCATC TATCCAGCTG AGGCAGTGCT TGTACCAATC 
TTACAGTCGG CATTTGCGAA ATTTTCTTTG AAAAGATTTT GGCTTCAAAA CTGGATAGGC 
CCGTCACTAG CAGGCTCATC TTTGTTTATG CACTGGGCTG GTGATTTTGA CTGCCTTGGT 
GCGTCTGAAA ATAAGAGTGA TGGGTTTTAT GAAAAAAATG GTTACAATAG CAGTGGTAGT 
AGCCGTAATA GCAGCATTAG TTCAACAAGT AGCGCTTCCA GCGGGAGCGG CTGGAGGATG 
ACGTCAAGAA CTGGTGATCT TGACGCTGAT GCAGATTCTT TGACGTGTAG GCAATCTGGT 
CTTACTTGTA ATGATGATCG CCTAAAAATG GGTTCCAAGC GGCCGCGAAC AGGGATGGCA 
GAGTCATTCG GTCAAGTAGG CATTGAAAAT GATCAAATTG GTTGGGATTG GGATGCTGAT 
GATGATGATG ATGACAGAGA AGTTGGCATG GATATCAAGG CACTTCTTTC AGAGTTTGGA 
GACTTTGGTG ACTTCTTTGA GAACGATGCT TTACCTTTCG GGGAGCCACC GGGAACTGCA 
GAGTCGCATG TGCTCGTTTT TCCTCCAGAT TCTGCTGATG TAGGTTCCAG TCCCGTGGAT 
ATGATGGATG TATCAGACCA AATCGTTTTG CCTGTTGGGT TTTCCTCTTT TGAGAGCTTT 
AATCCTGTTC CCCCAATCAT TGATGAGTGC CTTATTAAAA GTCAAGAAGT TCTCCACAGC 
AGTATCACTT CAGTTCCTTC AAATCAAATG TCGATCTCTT CGACTGGTGA GTTTGATCAT 
TTACTAAAAG CAGAAGCAAT GATGACCTTT GCTCCTGAGT ATGGAGCTGT AGAAGCACCT 
ATGAGCGAGA TTTCCTCGAC GTTTTTCAAA AGCCCATATC TTCCCAAATC TCATAAAGTG 
GAGAGTTCAA ATTCAAGAAC GAGTAATTAT GTCTATGGAG CCACACCACC GACCACTGAT 
TCTGATGGAG CAGGCGATAT GATTTTGTTC GGATCAAAAT CATGCATTGG AAACAATGCT 
GGCAGAACTC TGTATCATTC GAGGGAGCAT TACACTCAAG TAGAAGGTAG AAAGGGCCGA 
CATGATAAGC TACCTACAGT TATCAGTGAC AACAGTAGTA CAAAGGAGGG TGTCTCTCAA 
TCAATACACT CAAAACATAG TGCTGCGAAT GCTGTTAAGG TCGTACAGGG AAAAAAAACT 
GATGGTATAT CTGCTGTTGT TAGTACCTTA TTATCTTCAA AGACCCTGCT GGCAACAGAC 
GTGGGAAGTG TTATGTTCCA AGCTTTCATG TGTAGGATGC GGCACATAAT CACTTCTTCA 
AAGCACAGTT CACCTGTTAG TCTGACTAGG CTTAGCGGAA ACTTTTTCCT TAACCAGCTG 
TCAAATGAGC CTAGTACTCT GACAGACAAC ATATCTGCAA GGAACGAGAT ATATAAGAAA 
GATATACCTA CCAGAATAGC TGGAGATTTT GACGGAGGAA TGTTGGATAG CCACATGAGT 
GCACCAGTTG GCGTATGGCG GACTGTTTCA GTCCCAAAAA CAGCAAAACC TGCTAGTTCG 
CCAAATATTG AAGCAGGGTC ATCCTTGCCC CATAGTTCAT TTAGCGAAGA CAGCTTACTT 
TCTTATGGTC AAAGGCAACC ACTGCAGGAT CTCCTAGATG GAATAGCGCT ACTTGTACAG 
CAAGCTACCT CTTTTGTTGA TTTAGCTCTG GATTCAGATT GTGGAGATGG ACCTTATGGC 
TGGCTTGCAT TGGAAGAGTT ATGGAGACGG GAATTGTCGT GTGGTCCCTC TGCTGGCCAT 
GCAGGTTGTG GGGGAACTTT GGCTTCCTGC CATTCTCTGG ACATTGCTGG TGTCAAGCTT 
GTTGATCCAC TCTCTGCTGA GGTTTTTCCT TCGTCTGTCA TTACTCTTCT GCAATCTGAC 
ATCAAAACAG CTCTGAAATC TGCATTTGGT CAGTCAGATG GTCCATTATC TGTTACAGAT 
TGGTGCAAGG GCCGAAATCA ATCGGGGGAT GGAGGATCTA TTTCTGAGGG ATTTACTGCT 
GAGTCAGCCC TGAGTGAAGT CTCAAATGCA ATAGATGGTG GGAAAGGAGA TGAGACGGCT 
CAGAGCCAAG ACATATACAG TTCAGAATTA CTCCGACCGA CACTTTTTGT TCTTCCATCA 
CCTGCTATAC TTGTCGGGTA CCAAGATGAC TGGCTTAAGA TATCAACAAA CTCCTTGACA 
CATTGGGAAA AGGCCCCCTT TGAGCCATAT GCTCTGCCAA AAAGTATTAA TTATGCTGTT 
GTATGCCCGG ATATTGATCC TTTAACTTGT GCTGCTACTG ATTTCTTCCA GCAGCTTGGA 
ACTGTTTATG AAACGTGCAG GCTGGGAACA CATTTGCCAC AGAGCCTGGG AAATCAAATG 
GAAAAGGACG TTGGGAGATT ATCCTCATCT GGATTTGTTC TGCTTGATTG CCCTCAATCA 
ATGAAGATTG AAAGCAATAA CACATCGCTT CTGGGATCTC TCAGCGATTA TTTTCTATCT 
TTATCAAATG GGTGGAATGT GAATAGCTAT CTGAAGTCTT TATCAAAAGC GCTGAAAGGT 
CTAAAGCTTG GATCAGGCCT TTACACAAAC CAAAAAGAAG GATCAGGAAG TCCTTGTGTG 
GTAGTGTACA TAGTGTGTCC ATTTCCTGAC CCTTCGGCAG TTTTAAGAAC GATCGTCGAA 
TCGTCTATAG CACTTGGATC AGTAATACAG TCAGATAGAG ACAGGAGATC AATACTCAAC 
AGTCAAGTTG CAAGGGCGTT TAGTAGTTCT ACTGCTGTTG ACGAAGCATC AATATCTCAC 
ATTCCAGTGC TCTCAGGGTT TAGTGTCCCC AAATTAGTTC TACAAGTGGT GTCTGTTGAT 
TCAATTTTCC GGATAACAAG TCCAAGCTTT AATGAGCTTG TCATTCTCAA GGATACTGCT 
TTTTCTGTAT ACAATAAAGC TAGGAGAATT TCACGAGGAA TGCCTAATGA TGCATTTTTC 
TCATCATCTT TACCAAGCAG ATCGTCTTCA GCGTTGACAC CAATGAACTC TATTTCAGGA 
ATCTGGAAAG ACTGCGGTGG TTCTCGAATG ACAGGTTCTA CACATCCAAG AGATGGTGAA 
ATTGATGTTA GTTTGAGAAC GAGTGGTTGG GATACCTCTA CTTCCTGGCA GATACCAAGA 
TCTGGAGGAT TAAGCTGCGA CCCAAACAGA AATGGAGATT TTTACCTTAA TGATGAAATT 
TTCTATTTAT TTGAACCACT TTTCATTCTT TCCGAGCCTG GTTCTGTGGA GCGTGGAGTT 
TCACCTACTT TCACCAGCTT GGGTTCTGAG TCTTCCAAGC CAATACCCGA GGATGGTGGC 
AGAGGTTCTG GACCTGGCAT GAATTCAATG GAAGGTATAA CATCGGGATC AAGCTCCCAG 
GGGGATGTAT CCCAGCTTGA AGGAAAGGCT ATTCCAAGTT TACATTGCTG CTACGGTTGG 
ACAGAGGACT GGAGATGGCT TGTAAGCATC TGGACGGATG CCAGGGGTGA GTTGCTTGAC 
ACACATATAT TTCCCTTTGG TGGAATTAGC AGTAGACAGG ATACAAAAGG GCTGCAGTGT 
CTTTTCGTTC AAGTTCTGCA GCAGGGATGT CAAATACTAC AGGCGTGCTC CTCCCCTGAC 
AATGGATCTT TCAAACCCAG GGATTTTGTC ATTACACGCA TTGGGAATTT CTTCGAGCTT 
GAATACCAAG AGTGGCAAAA GGCAATTTAC TCAGCTGGAG GTCCTGAGAT TAAAAAGTGG 
CCTATTCAAC TGCGACGTTC TGCGCCTTCT GGCATAGCCA CCAACAGCAA CGGATCTTCC 
TTGCAACAGC AGGATCTGAG TCTGATTCAA GAGAGAGCCT CATCAACTAG CACACTCTAC 
AGCTCTCATT CAAAACAATC CACCTTTGTG AAAGGCAGTA TGGGGCAATC CGCTGGAAGA 
AAGCAGATAA TGGGTGGGCA GACCATTTCT GGTACTCCAA GGGGATTGTT TCAGTGGGTT 
CACAGCATCA GTTTTGCTTC TATTTCACTT GACCATTCCC TGCATTTTGT TCTTCCAGCT 
GAGTTGGTAT CCGCTGGTGG TGGCCAGAGC AGTACTGGTA TGAGTTCCGT TAATTACATT 
GAAGGTTTCA CTCCTGTCAA GTCTCTTGGT TCTACCGCTT TCTCTTACAT GATGATACCA 
TCACCCAACA TGCGCTTTCT TCACCCAAGT CCTCTTCAGC TTCCGACATG TTTAACTGCC 
GAGTCACCTC CACTTGCTCA CCTCCTTCAC AGCAAAGGCT ATGCAATCCC CTTGTCTACT 
GGATTTGTTG TTTCAAAAGC TGTGCCTTCC ATGAGAAAAG ACTCAAGGAT AAACGTGAAA 
GAAGAATGGC CATCAGTTCT TTCTGTAAGT CTCATAGACT ATTATGGTGG TTATGACAAC 
GCTCATGACA AAATTCTTCA GGGAATCGTG AAGCAGGGAG GAGGGACCAA AGAAACTAGA 
GATTTTGAGG TTGAAAGCCA TCTTATCCTT GAGTCAATTG CGGCAGAACT CCATGCTCTA 
TCATGGATGA CTGTTAGTCC AGCATATCTG GACAGGCGGA CGGCATTGCC GTTTCACTGT 
GATATGGTTC TGAGATTGAG ACGTCTTCTT CACTTTGCGG ACAAAGAAGT CTCCAGGATA 
CCTGATAAGA CAGGAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCT ( NM_179478.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_849809.2 Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.

Price & Availability↑

CloneID	OAb26459
Clone ID Related Accession (Same CDS sequence)	NM_179478.2
Accession Version	NM_179478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNA polymerase II transcription mediator
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Feb 17, 2004 this sequence version replaced NM_179478.1.

ATGTGGACTA ATGTTTTCAG AATTGGTGGT CTACATAATG TTTCTTGGTT TCAGTTTCTT 
CCAAGTGAAA CTGAGCTGAA TCCTGGCTTT GATAGAAGTT CAAGAGCTGA GCAGAATGAA 
GTTGCAACAT ATCTTGTACT TTCCTCTCAT CTACGGTTGC AGAAGGAAGG GTTTCTTACC 
ACTTGGACCA ATTCTTTTGT TGGACCTTGG GATCCATCTC AAGGTTTATA CAACCCTGAT 
GAAAAGATTA AGCTTTGGCT TTTTCTACCT GGGCGTCATT CATCAATATC TGATAAAGCT 
CAGGCTGCTG TGTCAAAGCT TAGAGTTGTT GCGTCAGGAA TTTGGGTAGC ACCTGGGGAC 
TCTGAAGAGA TATCAGTTGC CTTTTCACAG TCTTTACGTA ATTGCATCGA AAGAGCATTA 
TCTGGAATTT CCTACATGAG ATTTGGAGAT GTGTTTTCAA AATTCAGCCC TCAAAGCGAA 
GAATATTTGA GGAGGGGACA GCCTACTGTT GAATTCATCT TTGCTGCTAC CGAAGAGGCA 
GTTTTTGTCC ATGTCATTAT ATCTGCCAAG AATGTCCGCA CACTTTCAAG TGGCGATGCT 
GAAAGAATGT TAAGGAGTTC TTTGAAAAAC TCCAGTTATA GGCTTCCAGC TTTTCGTAAA 
TGTTTGGGAT TGGCCAAGAG TGAGGATAAT AGATTATGCT ACATAAACAC TTCTCACAGG 
CCTATGTTAT TCCCACCAGT TATTGTTTCT CCTCATGGAA TGCGGGGCAG CCTTACTGGA 
TTCTGTCCCA ATGACCTTGT CAAGCAAGTC TACTTCAGTT CTGGAAACTT AAAAACTTCG 
ACTGGATATG TTGGTCTCCC TTCTCATATT GGTCGTGGGT CCCGTCTGAT AAATGGCAAT 
CATTGCTACG TGGAAGTTAC ACTTGGTTGC TGCCAAAATA GAAATGACAA CACAAGTCAA 
GCTAATTCGA CCTTTGCAGT TAACTTGCCC CATAACCAGT GTCCTGAACC TTCAGTTGGG 
AGTAAAGATC ACCGTAAAGG ACAATCAGAT CTTTCATCAG TTTGTGAAAA AAAGTTCATC 
TATCCAGCTG AGGCAGTGCT TGTACCAATC TTACAGTCGG CATTTGCGAA ATTTTCTTTG 
AAAAGGGCTG GTGATTTTGA CTGCCTTGGT GCGTCTGAAA ATAAGAGTGA TGGGTTTTAT 
GAAAAAAATG GTTACAATAG CAGTGGTAGT AGCCGTAATA GCAGCATTAG TTCAACAAGT 
AGCGCTTCCA GCGGGAGCGG CTGGAGGATG ACGTCAAGAA CTGGTGATCT TGACGCTGAT 
GCAGATTCTT TGACGTGTAG GCAATCTGGT CTTACTTGTA ATGATGATCG CCTAAAAATG 
GGTTCCAAGC GGCCGCGAAC AGGGATGGCA GAGTCATTCG GTCAAGTAGG CATTGAAAAT 
GATCAAATTG GTTGGGATTG GGATGCTGAT GATGATGATG ATGACAGAGA AGTTGGCATG 
GATATCAAGG CACTTCTTTC AGAGTTTGGA GACTTTGGTG ACTTCTTTGA GAACGATGCT 
TTACCTTTCG GGGAGCCACC GGGAACTGCA GAGTCGCATG TGCTCGTTTT TCCTCCAGAT 
TCTGCTGATG TAGGTTCCAG TCCCGTGGAT ATGATGGATG TATCAGACCA AATCGTTTTG 
CCTGTTGGGT TTTCCTCTTT TGAGAGCTTT AATCCTGTTC CCCCAATCAT TGATGAGTGC 
CTTATTAAAA GTCAAGAAGT TCTCCACAGC AGTATCACTT CAGTTCCTTC AAATCAAATG 
TCGATCTCTT CGACTGGTGA GTTTGATCAT TTACTAAAAG CAGAAGCAAT GATGACCTTT 
GCTCCTGAGT ATGGAGCTGT AGAAGCACCT ATGAGCGAGA TTTCCTCGAC GTTTTTCAAA 
AGCCCATATC TTCCCAAATC TCATAAAGTG GAGAGTTCAA ATTCAAGAAC GAGTAATTAT 
GTCTATGGAG CCACACCACC GACCACTGAT TCTGATGGAG CAGGCGATAT GATTTTGTTC 
GGATCAAAAT CATGCATTGG AAACAATGCT GGCAGAACTC TGTATCATTC GAGGGAGCAT 
TACACTCAAG TAGAAGGTAG AAAGGGCCGA CATGATAAGC TACCTACAGT TATCAGTGAC 
AACAGTAGTA CAAAGGAGGG TGTCTCTCAA TCAATACACT CAAAACATAG TGCTGCGAAT 
GCTGTTAAGG TCGTACAGGG AAAAAAAACT GATGGTATAT CTGCTGTTGT TAGTACCTTA 
TTATCTTCAA AGACCCTGCT GGCAACAGAC GTGGGAAGTG TTATGTTCCA AGCTTTCATG 
TGTAGGATGC GGCACATAAT CACTTCTTCA AAGCACAGTT CACCTGTTAG TCTGACTAGG 
CTTAGCGGAA ACTTTTTCCT TAACCAGCTG TCAAATGAGC CTAGTACTCT GACAGACAAC 
ATATCTGCAA GGAACGAGAT ATATAAGAAA GATATACCTA CCAGAATAGC TGGAGATTTT 
GACGGAGGAA TGTTGGATAG CCACATGAGT GCACCAGTTG GCGTATGGCG GACTGTTTCA 
GTCCCAAAAA CAGCAAAACC TGCTAGTTCG CCAAATATTG AAGCAGGGTC ATCCTTGCCC 
CATAGTTCAT TTAGCGAAGA CAGCTTACTT TCTTATGGTC AAAGGCAACC ACTGCAGGAT 
CTCCTAGATG GAATAGCGCT ACTTGTACAG CAAGCTACCT CTTTTGTTGA TTTAGCTCTG 
GATTCAGATT GTGGAGATGG ACCTTATGGC TGGCTTGCAT TGGAAGAGTT ATGGAGACGG 
GAATTGTCGT GTGGTCCCTC TGCTGGCCAT GCAGGTTGTG GGGGAACTTT GGCTTCCTGC 
CATTCTCTGG ACATTGCTGG TGTCAAGCTT GTTGATCCAC TCTCTGCTGA GGTTTTTCCT 
TCGTCTGTCA TTACTCTTCT GCAATCTGAC ATCAAAACAG CTCTGAAATC TGCATTTGGT 
CAGTCAGATG GTCCATTATC TGTTACAGAT TGGTGCAAGG GCCGAAATCA ATCGGGGGAT 
GGAGGATCTA TTTCTGAGGG ATTTACTGCT GAGTCAGCCC TGAGTGAAGT GAATGGTGTC 
AATATCTCTG ATGATTTTAT TATTGATAAA TATTTTGGCA AACAAGCAGT CTCAAATGCA 
ATAGATGGTG GGAAAGGAGA TGAGACGGCT CAGAGCCAAG ACATATACAG TTCAGAATTA 
CTCCGACCGA CACTTTTTGT TCTTCCATCA CCTGCTATAC TTGTCGGGTA CCAAGATGAC 
TGGCTTAAGA TATCAACAAA CTCCTTGACA CATTGGGAAA AGGCCCCCTT TGAGCCATAT 
GCTCTGCCAA AAAGTATTAA TTATGCTGTT GTATGCCCGG ATATTGATCC TTTAACTTGT 
GCTGCTACTG ATTTCTTCCA GCAGCTTGGA ACTGGTGAGT CAACGAGTTT ATCTTTTGTG 
AAAGAGGTTA TGCTCAAACA TGATAGGAAC TGGCTGGGAA CACATTTGCC ACAGAGCCTG 
GGAAATCAAA TGGAAAAGGA CGTTGGGAGA TTATCCTCAT CTGGATTTGT TCTGCTTGAT 
TGCCCTCAAT CAATGAAGAT TGAAAGCAAT AACACATCGC TTCTGGGATC TCTCAGCGAT 
TATTTTCTAT CTTTATCAAA TGGGTGGAAT GTGAATAGCT ATCTGAAGTC TTTATCAAAA 
GCGCTGAAAG GTCTAAAGCT TGGATCAGGC CTTTACACAA ACCAAAAAGA AGGATCAGGA 
AGTCCTTGTG TGGTAGTGTA CATAGTGTGT CCATTTCCTG ACCCTTCGGC AGTTTTAAGA 
ACGATCGTCG AATCGTCTAT AGCACTTGGA TCAGTAATAC AGTCAGATAG AGACAGGAGA 
TCAATACTCA ACAGTCAAGT TGCAAGGGCG TTTAGTAGTT CTACTGCTGT TGACGAAGCA 
TCAATATCTC ACATTCCAGT GCTCTCAGGG TTTAGTGTCC CCAAATTAGT TCTACAAGTG 
GTGTCTGTTG ATTCAATTTT CCGGATAACA AGTCCAAGCT TTAATGAGCT TGTCATTCTC 
AAGGATACTG CTTTTTCTGT ATACAATAAA GCTAGGAGAA TTTCACGAGG AATGCCTAAT 
GATGCATTTT TCTCATCATC TTTACCAAGC AGATCGTCTT CAGCGTTGAC ACCAATGAAC 
TCTATTTCAG GAATCTGGAA AGACTGCGGT GGTTCTCGAA TGACAGGTTC TACACATCCA 
AGAGATGGTG AAATTGATGT TAGTTTGAGA ACGAGTGGTT GGGATACCTC TACTTCCTGG 
CAGATACCAA GATCTGGAGG ATTAAGCTGC GACCCAAACA GAAATGGAGA TTTTTACCTT 
AATGATGAAA TTTTCTATTT ATTTGAACCA CTTTTCATTC TTTCCGAGCC TGGTTCTGTG 
GAGCGTGGAG TTTCACCTAC TTTCACCAGC TTGGGTTCTG AGTCTTCCAA GCCAATACCC 
GAGGATGGTG GCAGAGGTTC TGGACCTGGC ATGAATTCAA TGGAAGGTAT AACATCGGGA 
TCAAGCTCCC AGGGGGATGT ATCCCAGCTT GAAGGAAAGG CTATTCCAAG TTTACATTGC 
TGCTACGGTT GGACAGAGGA CTGGAGATGG CTTGTAAGCA TCTGGACGGA TGCCAGGGTT 
CTGCAGCAGG GATGTCAAAT ACTACAGGCG TGCTCCTCCC CTGACAATGG ATCTTTCAAA 
CCCAGGGATT TTGTCATTAC ACGCATTGGG AATTTCTTCG AGCTTGAATA CCAAGAGTGG 
CAAAAGGCAA TTTACTCAGC TGGAGGTCCT GAGATTAAAA AGTGGCCTAT TCAACTGCGA 
CGTTCTGCGC CTTCTGGCAT AGCCACCAAC AGCAACGGAT CTTCCTTGCA ACAGCAGGAT 
CTGAGTCTGA TTCAAGAGAG AGCCTCATCA ACTAGCACAC TCTACAGCTC TCATTCAAAA 
CAATCCACCT TTGTGAAAGG CAGTATGGGG CAATCCGCTG GAAGAAAGCA GATAATGGGT 
GGGCAGACCA TTTCTGGTAC TCCAAGGGGA TTGTTTCAGT GGGTTCACAG CATCAGTTTT 
GCTTCTATTT CACTTGACCA TTCCCTGCAT TTTGTTCTTC CAGCTGAGTT GGTATCCGCT 
GGTGGTGGCC AGAGCAGTAC TGGTATGAGT TCCGTTAATT ACATTGAAGG TTTCACTCCT 
GTCAAGTCTC TTGGTTCTAC CGCTTTCTCT TACATGATGA TACCATCACC CAACATGCGC 
TTTCTTCACC CAAGTCCTCT TCAGCTTCCG ACATGTTTAA CTGCCGAGTC ACCTCCACTT 
GCTCACCTCC TTCACAGCAA AGGCTATGCA ATCCCCTTGT CTACTGGATT TGTTGTTTCA 
AAAGCTGTGC CTTCCATGAG AAAAGACTCA AGGATAAACG TGAAAGAAGA ATGGCCATCA 
GTTCTTTCTG TAAGTCTCAT AGACTATTAT GGTGGTTATG ACAACGCTCA TGACAAAATT 
CTTCAGGGAA TCGTGAAGCA GGGAGGAGGG ACCAAAGAAA CTAGAGATTT TGAGGTTGAA 
AGCCATCTTA TCCTTGAGTC AATTGCGGCA GAACTCCATG CTCTATCATG GATGACTGTT 
AGTCCAGCAT ATCTGGACAG GCGGACGGCA TTGCCGTTTC ACTGTGATAT GGTTCTGAGA 
TTGAGACGTC TTCTTCACTT TGCGGACAAA GAAGTCTCCA GGATACCTGA TAAGACAGGA 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849809.2
CDS	158..5923
Translation	MWTNVFRIGGLHNVSWFQFLPSETELNPGFDRSSRAEQNEVATYLVLSSHLRLQKEGFLTTWTNSFVGPWDPSQGLYNPDEKIKLWLFLPGRHSSISDKAQAAVSKLRVVASGIWVAPGDSEEISVAFSQSLRNCIERALSGISYMRFGDVFSKFSPQSEEYLRRGQPTVEFIFAATEEAVFVHVIISAKNVRTLSSGDAERMLRSSLKNSSYRLPAFRKCLGLAKSEDNRLCYINTSHRPMLFPPVIVSPHGMRGSLTGFCPNDLVKQVYFSSGNLKTSTGYVGLPSHIGRGSRLINGNHCYVEVTLGCCQNRNDNTSQANSTFAVNLPHNQCPEPSVGSKDHRKGQSDLSSVCEKKFIYPAEAVLVPILQSAFAKFSLKRAGDFDCLGASENKSDGFYEKNGYNSSGSSRNSSISSTSSASSGSGWRMTSRTGDLDADADSLTCRQSGLTCNDDRLKMGSKRPRTGMAESFGQVGIENDQIGWDWDADDDDDDREVGMDIKALLSEFGDFGDFFENDALPFGEPPGTAESHVLVFPPDSADVGSSPVDMMDVSDQIVLPVGFSSFESFNPVPPIIDECLIKSQEVLHSSITSVPSNQMSISSTGEFDHLLKAEAMMTFAPEYGAVEAPMSEISSTFFKSPYLPKSHKVESSNSRTSNYVYGATPPTTDSDGAGDMILFGSKSCIGNNAGRTLYHSREHYTQVEGRKGRHDKLPTVISDNSSTKEGVSQSIHSKHSAANAVKVVQGKKTDGISAVVSTLLSSKTLLATDVGSVMFQAFMCRMRHIITSSKHSSPVSLTRLSGNFFLNQLSNEPSTLTDNISARNEIYKKDIPTRIAGDFDGGMLDSHMSAPVGVWRTVSVPKTAKPASSPNIEAGSSLPHSSFSEDSLLSYGQRQPLQDLLDGIALLVQQATSFVDLALDSDCGDGPYGWLALEELWRRELSCGPSAGHAGCGGTLASCHSLDIAGVKLVDPLSAEVFPSSVITLLQSDIKTALKSAFGQSDGPLSVTDWCKGRNQSGDGGSISEGFTAESALSEVNGVNISDDFIIDKYFGKQAVSNAIDGGKGDETAQSQDIYSSELLRPTLFVLPSPAILVGYQDDWLKISTNSLTHWEKAPFEPYALPKSINYAVVCPDIDPLTCAATDFFQQLGTGESTSLSFVKEVMLKHDRNWLGTHLPQSLGNQMEKDVGRLSSSGFVLLDCPQSMKIESNNTSLLGSLSDYFLSLSNGWNVNSYLKSLSKALKGLKLGSGLYTNQKEGSGSPCVVVYIVCPFPDPSAVLRTIVESSIALGSVIQSDRDRRSILNSQVARAFSSSTAVDEASISHIPVLSGFSVPKLVLQVVSVDSIFRITSPSFNELVILKDTAFSVYNKARRISRGMPNDAFFSSSLPSRSSSALTPMNSISGIWKDCGGSRMTGSTHPRDGEIDVSLRTSGWDTSTSWQIPRSGGLSCDPNRNGDFYLNDEIFYLFEPLFILSEPGSVERGVSPTFTSLGSESSKPIPEDGGRGSGPGMNSMEGITSGSSSQGDVSQLEGKAIPSLHCCYGWTEDWRWLVSIWTDARVLQQGCQILQACSSPDNGSFKPRDFVITRIGNFFELEYQEWQKAIYSAGGPEIKKWPIQLRRSAPSGIATNSNGSSLQQQDLSLIQERASSTSTLYSSHSKQSTFVKGSMGQSAGRKQIMGGQTISGTPRGLFQWVHSISFASISLDHSLHFVLPAELVSAGGGQSSTGMSSVNYIEGFTPVKSLGSTAFSYMMIPSPNMRFLHPSPLQLPTCLTAESPPLAHLLHSKGYAIPLSTGFVVSKAVPSMRKDSRINVKEEWPSVLSVSLIDYYGGYDNAHDKILQGIVKQGGGTKETRDFEVESHLILESIAAELHALSWMTVSPAYLDRRTALPFHCDMVLRLRRLLHFADKEVSRIPDKTGV

Target ORF information:

RefSeq Version	NM_179478.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179478.2

ORF Insert Sequence:

ATGTGGACTA ATGTTTTCAG AATTGGTGGT CTACATAATG TTTCTTGGTT TCAGTTTCTT 
CCAAGTGAAA CTGAGCTGAA TCCTGGCTTT GATAGAAGTT CAAGAGCTGA GCAGAATGAA 
GTTGCAACAT ATCTTGTACT TTCCTCTCAT CTACGGTTGC AGAAGGAAGG GTTTCTTACC 
ACTTGGACCA ATTCTTTTGT TGGACCTTGG GATCCATCTC AAGGTTTATA CAACCCTGAT 
GAAAAGATTA AGCTTTGGCT TTTTCTACCT GGGCGTCATT CATCAATATC TGATAAAGCT 
CAGGCTGCTG TGTCAAAGCT TAGAGTTGTT GCGTCAGGAA TTTGGGTAGC ACCTGGGGAC 
TCTGAAGAGA TATCAGTTGC CTTTTCACAG TCTTTACGTA ATTGCATCGA AAGAGCATTA 
TCTGGAATTT CCTACATGAG ATTTGGAGAT GTGTTTTCAA AATTCAGCCC TCAAAGCGAA 
GAATATTTGA GGAGGGGACA GCCTACTGTT GAATTCATCT TTGCTGCTAC CGAAGAGGCA 
GTTTTTGTCC ATGTCATTAT ATCTGCCAAG AATGTCCGCA CACTTTCAAG TGGCGATGCT 
GAAAGAATGT TAAGGAGTTC TTTGAAAAAC TCCAGTTATA GGCTTCCAGC TTTTCGTAAA 
TGTTTGGGAT TGGCCAAGAG TGAGGATAAT AGATTATGCT ACATAAACAC TTCTCACAGG 
CCTATGTTAT TCCCACCAGT TATTGTTTCT CCTCATGGAA TGCGGGGCAG CCTTACTGGA 
TTCTGTCCCA ATGACCTTGT CAAGCAAGTC TACTTCAGTT CTGGAAACTT AAAAACTTCG 
ACTGGATATG TTGGTCTCCC TTCTCATATT GGTCGTGGGT CCCGTCTGAT AAATGGCAAT 
CATTGCTACG TGGAAGTTAC ACTTGGTTGC TGCCAAAATA GAAATGACAA CACAAGTCAA 
GCTAATTCGA CCTTTGCAGT TAACTTGCCC CATAACCAGT GTCCTGAACC TTCAGTTGGG 
AGTAAAGATC ACCGTAAAGG ACAATCAGAT CTTTCATCAG TTTGTGAAAA AAAGTTCATC 
TATCCAGCTG AGGCAGTGCT TGTACCAATC TTACAGTCGG CATTTGCGAA ATTTTCTTTG 
AAAAGGGCTG GTGATTTTGA CTGCCTTGGT GCGTCTGAAA ATAAGAGTGA TGGGTTTTAT 
GAAAAAAATG GTTACAATAG CAGTGGTAGT AGCCGTAATA GCAGCATTAG TTCAACAAGT 
AGCGCTTCCA GCGGGAGCGG CTGGAGGATG ACGTCAAGAA CTGGTGATCT TGACGCTGAT 
GCAGATTCTT TGACGTGTAG GCAATCTGGT CTTACTTGTA ATGATGATCG CCTAAAAATG 
GGTTCCAAGC GGCCGCGAAC AGGGATGGCA GAGTCATTCG GTCAAGTAGG CATTGAAAAT 
GATCAAATTG GTTGGGATTG GGATGCTGAT GATGATGATG ATGACAGAGA AGTTGGCATG 
GATATCAAGG CACTTCTTTC AGAGTTTGGA GACTTTGGTG ACTTCTTTGA GAACGATGCT 
TTACCTTTCG GGGAGCCACC GGGAACTGCA GAGTCGCATG TGCTCGTTTT TCCTCCAGAT 
TCTGCTGATG TAGGTTCCAG TCCCGTGGAT ATGATGGATG TATCAGACCA AATCGTTTTG 
CCTGTTGGGT TTTCCTCTTT TGAGAGCTTT AATCCTGTTC CCCCAATCAT TGATGAGTGC 
CTTATTAAAA GTCAAGAAGT TCTCCACAGC AGTATCACTT CAGTTCCTTC AAATCAAATG 
TCGATCTCTT CGACTGGTGA GTTTGATCAT TTACTAAAAG CAGAAGCAAT GATGACCTTT 
GCTCCTGAGT ATGGAGCTGT AGAAGCACCT ATGAGCGAGA TTTCCTCGAC GTTTTTCAAA 
AGCCCATATC TTCCCAAATC TCATAAAGTG GAGAGTTCAA ATTCAAGAAC GAGTAATTAT 
GTCTATGGAG CCACACCACC GACCACTGAT TCTGATGGAG CAGGCGATAT GATTTTGTTC 
GGATCAAAAT CATGCATTGG AAACAATGCT GGCAGAACTC TGTATCATTC GAGGGAGCAT 
TACACTCAAG TAGAAGGTAG AAAGGGCCGA CATGATAAGC TACCTACAGT TATCAGTGAC 
AACAGTAGTA CAAAGGAGGG TGTCTCTCAA TCAATACACT CAAAACATAG TGCTGCGAAT 
GCTGTTAAGG TCGTACAGGG AAAAAAAACT GATGGTATAT CTGCTGTTGT TAGTACCTTA 
TTATCTTCAA AGACCCTGCT GGCAACAGAC GTGGGAAGTG TTATGTTCCA AGCTTTCATG 
TGTAGGATGC GGCACATAAT CACTTCTTCA AAGCACAGTT CACCTGTTAG TCTGACTAGG 
CTTAGCGGAA ACTTTTTCCT TAACCAGCTG TCAAATGAGC CTAGTACTCT GACAGACAAC 
ATATCTGCAA GGAACGAGAT ATATAAGAAA GATATACCTA CCAGAATAGC TGGAGATTTT 
GACGGAGGAA TGTTGGATAG CCACATGAGT GCACCAGTTG GCGTATGGCG GACTGTTTCA 
GTCCCAAAAA CAGCAAAACC TGCTAGTTCG CCAAATATTG AAGCAGGGTC ATCCTTGCCC 
CATAGTTCAT TTAGCGAAGA CAGCTTACTT TCTTATGGTC AAAGGCAACC ACTGCAGGAT 
CTCCTAGATG GAATAGCGCT ACTTGTACAG CAAGCTACCT CTTTTGTTGA TTTAGCTCTG 
GATTCAGATT GTGGAGATGG ACCTTATGGC TGGCTTGCAT TGGAAGAGTT ATGGAGACGG 
GAATTGTCGT GTGGTCCCTC TGCTGGCCAT GCAGGTTGTG GGGGAACTTT GGCTTCCTGC 
CATTCTCTGG ACATTGCTGG TGTCAAGCTT GTTGATCCAC TCTCTGCTGA GGTTTTTCCT 
TCGTCTGTCA TTACTCTTCT GCAATCTGAC ATCAAAACAG CTCTGAAATC TGCATTTGGT 
CAGTCAGATG GTCCATTATC TGTTACAGAT TGGTGCAAGG GCCGAAATCA ATCGGGGGAT 
GGAGGATCTA TTTCTGAGGG ATTTACTGCT GAGTCAGCCC TGAGTGAAGT GAATGGTGTC 
AATATCTCTG ATGATTTTAT TATTGATAAA TATTTTGGCA AACAAGCAGT CTCAAATGCA 
ATAGATGGTG GGAAAGGAGA TGAGACGGCT CAGAGCCAAG ACATATACAG TTCAGAATTA 
CTCCGACCGA CACTTTTTGT TCTTCCATCA CCTGCTATAC TTGTCGGGTA CCAAGATGAC 
TGGCTTAAGA TATCAACAAA CTCCTTGACA CATTGGGAAA AGGCCCCCTT TGAGCCATAT 
GCTCTGCCAA AAAGTATTAA TTATGCTGTT GTATGCCCGG ATATTGATCC TTTAACTTGT 
GCTGCTACTG ATTTCTTCCA GCAGCTTGGA ACTGGTGAGT CAACGAGTTT ATCTTTTGTG 
AAAGAGGTTA TGCTCAAACA TGATAGGAAC TGGCTGGGAA CACATTTGCC ACAGAGCCTG 
GGAAATCAAA TGGAAAAGGA CGTTGGGAGA TTATCCTCAT CTGGATTTGT TCTGCTTGAT 
TGCCCTCAAT CAATGAAGAT TGAAAGCAAT AACACATCGC TTCTGGGATC TCTCAGCGAT 
TATTTTCTAT CTTTATCAAA TGGGTGGAAT GTGAATAGCT ATCTGAAGTC TTTATCAAAA 
GCGCTGAAAG GTCTAAAGCT TGGATCAGGC CTTTACACAA ACCAAAAAGA AGGATCAGGA 
AGTCCTTGTG TGGTAGTGTA CATAGTGTGT CCATTTCCTG ACCCTTCGGC AGTTTTAAGA 
ACGATCGTCG AATCGTCTAT AGCACTTGGA TCAGTAATAC AGTCAGATAG AGACAGGAGA 
TCAATACTCA ACAGTCAAGT TGCAAGGGCG TTTAGTAGTT CTACTGCTGT TGACGAAGCA 
TCAATATCTC ACATTCCAGT GCTCTCAGGG TTTAGTGTCC CCAAATTAGT TCTACAAGTG 
GTGTCTGTTG ATTCAATTTT CCGGATAACA AGTCCAAGCT TTAATGAGCT TGTCATTCTC 
AAGGATACTG CTTTTTCTGT ATACAATAAA GCTAGGAGAA TTTCACGAGG AATGCCTAAT 
GATGCATTTT TCTCATCATC TTTACCAAGC AGATCGTCTT CAGCGTTGAC ACCAATGAAC 
TCTATTTCAG GAATCTGGAA AGACTGCGGT GGTTCTCGAA TGACAGGTTC TACACATCCA 
AGAGATGGTG AAATTGATGT TAGTTTGAGA ACGAGTGGTT GGGATACCTC TACTTCCTGG 
CAGATACCAA GATCTGGAGG ATTAAGCTGC GACCCAAACA GAAATGGAGA TTTTTACCTT 
AATGATGAAA TTTTCTATTT ATTTGAACCA CTTTTCATTC TTTCCGAGCC TGGTTCTGTG 
GAGCGTGGAG TTTCACCTAC TTTCACCAGC TTGGGTTCTG AGTCTTCCAA GCCAATACCC 
GAGGATGGTG GCAGAGGTTC TGGACCTGGC ATGAATTCAA TGGAAGGTAT AACATCGGGA 
TCAAGCTCCC AGGGGGATGT ATCCCAGCTT GAAGGAAAGG CTATTCCAAG TTTACATTGC 
TGCTACGGTT GGACAGAGGA CTGGAGATGG CTTGTAAGCA TCTGGACGGA TGCCAGGGTT 
CTGCAGCAGG GATGTCAAAT ACTACAGGCG TGCTCCTCCC CTGACAATGG ATCTTTCAAA 
CCCAGGGATT TTGTCATTAC ACGCATTGGG AATTTCTTCG AGCTTGAATA CCAAGAGTGG 
CAAAAGGCAA TTTACTCAGC TGGAGGTCCT GAGATTAAAA AGTGGCCTAT TCAACTGCGA 
CGTTCTGCGC CTTCTGGCAT AGCCACCAAC AGCAACGGAT CTTCCTTGCA ACAGCAGGAT 
CTGAGTCTGA TTCAAGAGAG AGCCTCATCA ACTAGCACAC TCTACAGCTC TCATTCAAAA 
CAATCCACCT TTGTGAAAGG CAGTATGGGG CAATCCGCTG GAAGAAAGCA GATAATGGGT 
GGGCAGACCA TTTCTGGTAC TCCAAGGGGA TTGTTTCAGT GGGTTCACAG CATCAGTTTT 
GCTTCTATTT CACTTGACCA TTCCCTGCAT TTTGTTCTTC CAGCTGAGTT GGTATCCGCT 
GGTGGTGGCC AGAGCAGTAC TGGTATGAGT TCCGTTAATT ACATTGAAGG TTTCACTCCT 
GTCAAGTCTC TTGGTTCTAC CGCTTTCTCT TACATGATGA TACCATCACC CAACATGCGC 
TTTCTTCACC CAAGTCCTCT TCAGCTTCCG ACATGTTTAA CTGCCGAGTC ACCTCCACTT 
GCTCACCTCC TTCACAGCAA AGGCTATGCA ATCCCCTTGT CTACTGGATT TGTTGTTTCA 
AAAGCTGTGC CTTCCATGAG AAAAGACTCA AGGATAAACG TGAAAGAAGA ATGGCCATCA 
GTTCTTTCTG TAAGTCTCAT AGACTATTAT GGTGGTTATG ACAACGCTCA TGACAAAATT 
CTTCAGGGAA TCGTGAAGCA GGGAGGAGGG ACCAAAGAAA CTAGAGATTT TGAGGTTGAA 
AGCCATCTTA TCCTTGAGTC AATTGCGGCA GAACTCCATG CTCTATCATG GATGACTGTT 
AGTCCAGCAT ATCTGGACAG GCGGACGGCA TTGCCGTTTC ACTGTGATAT GGTTCTGAGA 
TTGAGACGTC TTCTTCACTT TGCGGACAAA GAAGTCTCCA GGATACCTGA TAAGACAGGA 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCT ( NM_001198308.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185237.1 Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.

Price & Availability↑

CloneID	OAb30062
Clone ID Related Accession (Same CDS sequence)	NM_001198308.1
Accession Version	NM_001198308.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNA polymerase II transcription mediator
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGTGGACTA ATGTTTTCAG AATTGGTGGT CTACATAATG TTTCTTGGTT TCAGTTTCTT 
CCAAGTGAAA CTGAGCTGAA TCCTGGCTTT GATAGAAGTT CAAGAGCTGA GCAGAATGAA 
GTTGCAACAT ATCTTGTACT TTCCTCTCAT CTACGGTTGC AGAAGGAAGG GTTTCTTACC 
ACTTGGACCA ATTCTTTTGT TGGACCTTGG GATCCATCTC AAGGTTTATA CAACCCTGAT 
GAAAAGATTA AGCTTTGGCT TTTTCTACCT GGGCGTCATT CATCAATATC TGATAAAGCT 
CAGGCTGCTG TGTCAAAGCT TAGAGTTGTT GCGTCAGGAA TTTGGGTAGC ACCTGGGGAC 
TCTGAAGAGA TATCAGTTGC CTTTTCACAG TCTTTACGTA ATTGCATCGA AAGAGCATTA 
TCTGGAATTT CCTACATGAG ATTTGGAGAT GTGTTTTCAA AATTCAGCCC TCAAAGCGAA 
GAATATTTGA GGAGGGGACA GCCTACTGTT GAATTCATCT TTGCTGCTAC CGAAGAGGCA 
GTTTTTGTCC ATGTCATTAT ATCTGCCAAG AATGTCCGCA CACTTTCAAG TGGCGATGCT 
GAAAGAATGT TAAGGAGTTC TTTGAAAAAC TCCAGTTATA GGCTTCCAGT TATTGTTTCT 
CCTCATGGAA TGCGGGGCAG CCTTACTGGA TTCTGTCCCA ATGACCTTGT CAAGCAAGTC 
TACTTCAGTT CTGGAAACTT AAAAACTTCG ACTGGATATG TTGGTCTCCC TTCTCATATT 
GGTCGTGGGT CCCGTCTGAT AAATGGCAAT CATTGCTACG TGGAAGTTAC ACTTGGTTGC 
TGCCAAAATA GAAATGACAA CACAAGTCAA GCTAATTCGA CCTTTGCAGT TAACTTGCCC 
CATAACCAGT GTCCTGAACC TTCAGTTGGG AGTAAAGATC ACCGTAAAGG ACAATCAGAT 
CTTTCATCAG TTTGTGAAAA AAAGTTCATC TATCCAGCTG AGGCAGTGCT TGTACCAATC 
TTACAGTCGG CATTTGCGAA ATTTTCTTTG AAAAGATTTT GGCTTCAAAA CTGGATAGGC 
CCGTCACTAG CAGGCTCATC TTTGTTTATG CACTGGGCTG GTGATTTTGA CTGCCTTGGT 
GCGTCTGAAA ATAAGAGTGA TGGGTTTTAT GAAAAAAATG GTTACAATAG CAGTGGTAGT 
AGCCGTAATA GCAGCATTAG TTCAACAAGT AGCGCTTCCA GCGGGAGCGG CTGGAGGATG 
ACGTCAAGAA CTGGTGATCT TGACGCTGAT GCAGATTCTT TGACGTGTAG GCAATCTGGT 
CTTACTTGTA ATGATGATCG CCTAAAAATG GGTTCCAAGC GGCCGCGAAC AGGGATGGCA 
GAGTCATTCG GTCAAGTAGG CATTGAAAAT GATCAAATTG GTTGGGATTG GGATGCTGAT 
GATGATGATG ATGACAGAGA AGTTGGCATG GATATCAAGG CACTTCTTTC AGAGTTTGGA 
GACTTTGGTG ACTTCTTTGA GAACGATGCT TTACCTTTCG GGGAGCCACC GGGAACTGCA 
GAGTCGCATG TGCTCGTTTT TCCTCCAGAT TCTGCTGATG TAGGTTCCAG TCCCGTGGAT 
ATGATGGATG TATCAGACCA AATCGTTTTG CCTGTTGGGT TTTCCTCTTT TGAGAGCTTT 
AATCCTGTTC CCCCAATCAT TGATGAGTGC CTTATTAAAA GTCAAGAAGT TCTCCACAGC 
AGTATCACTT CAGTTCCTTC AAATCAAATG TCGATCTCTT CGACTGGTGA GTTTGATCAT 
TTACTAAAAG CAGAAGCAAT GATGACCTTT GCTCCTGAGT ATGGAGCTGT AGAAGCACCT 
ATGAGCGAGA TTTCCTCGAC GTTTTTCAAA AGCCCATATC TTCCCAAATC TCATAAAGTG 
GAGAGTTCAA ATTCAAGAAC GAGTAATTAT GTCTATGGAG CCACACCACC GACCACTGAT 
TCTGATGGAG CAGGCGATAT GATTTTGTTC GGATCAAAAT CATGCATTGG AAACAATGCT 
GGCAGAACTC TGTATCATTC GAGGGAGCAT TACACTCAAG TAGAAGGTAG AAAGGGCCGA 
CATGATAAGC TACCTACAGT TATCAGTGAC AACAGTAGTA CAAAGGAGGG TGTCTCTCAA 
TCAATACACT CAAAACATAG TGCTGCGAAT GCTGTTAAGG TCGTACAGGG AAAAAAAACT 
GATGGTATAT CTGCTGTTGT TAGTACCTTA TTATCTTCAA AGACCCTGCT GGCAACAGAC 
GTGGGAAGTG TTATGTTCCA AGCTTTCATG TGTAGGATGC GGCACATAAT CACTTCTTCA 
AAGCACAGTT CACCTGTTAG TCTGACTAGG CTTAGCGGAA ACTTTTTCCT TAACCAGCTG 
TCAAATGAGC CTAGTACTCT GACAGACAAC ATATCTGCAA GGAACGAGAT ATATAAGAAA 
GATATACCTA CCAGAATAGC TGGAGATTTT GACGGAGGAA TGTTGGATAG CCACATGAGT 
GCACCAGTTG GCGTATGGCG GACTGTTTCA GTCCCAAAAA CAGCAAAACC TGCTAGTTCG 
CCAAATATTG AAGCAGGGTC ATCCTTGCCC CATAGTTCAT TTAGCGAAGA CAGCTTACTT 
TCTTATGGTC AAAGGCAACC ACTGCAGGAT CTCCTAGATG GAATAGCGCT ACTTGTACAG 
CAAGCTACCT CTTTTGTTGA TTTAGCTCTG GATTCAGATT GTGGAGATGG ACCTTATGGC 
TGGCTTGCAT TGGAAGAGTT ATGGAGACGG GAATTGTCGT GTGGTCCCTC TGCTGGCCAT 
GCAGGTTGTG GGGGAACTTT GGCTTCCTGC CATTCTCTGG ACATTGCTGG TGTCAAGCTT 
GTTGATCCAC TCTCTGCTGA GGTTTTTCCT TCGTCTGTCA TTACTCTTCT GCAATCTGAC 
ATCAAAACAG CTCTGAAATC TGCATTTGGT CAGTCAGATG GTCCATTATC TGTTACAGAT 
TGGTGCAAGG GCCGAAATCA ATCGGGGGAT GGAGGATCTA TTTCTGAGGG ATTTACTGCT 
GAGTCAGCCC TGAGTGAAGT CTCAAATGCA ATAGATGGTG GGAAAGGAGA TGAGACGGCT 
CAGAGCCAAG ACATATACAG TTCAGAATTA CTCCGACCGA CACTTTTTGT TCTTCCATCA 
CCTGCTATAC TTGTCGGGTA CCAAGATGAC TGGCTTAAGA TATCAACAAA CTCCTTGACA 
CATTGGGAAA AGGCCCCCTT TGAGCCATAT GCTCTGCCAA AAAGTATTAA TTATGCTGTT 
GTATGCCCGG ATATTGATCC TTTAACTTGT GCTGCTACTG ATTTCTTCCA GCAGCTTGGA 
ACTGTTTATG AAACGTGCAG GCTGGGAACA CATTTGCCAC AGAGCCTGGG AAATCAAATG 
GAAAAGGACG TTGGGAGATT ATCCTCATCT GGATTTGTTC TGCTTGATTG CCCTCAATCA 
ATGAAGATTG AAAGCAATAA CACATCGCTT CTGGGATCTC TCAGCGATTA TTTTCTATCT 
TTATCAAATG GGTGGAATGT GAATAGCTAT CTGAAGTCTT TATCAAAAGC GCTGAAAGGT 
CTAAAGCTTG GATCAGGCCT TTACACAAAC CAAAAAGAAG GATCAGGAAG TCCTTGTGTG 
GTAGTGTACA TAGTGTGTCC ATTTCCTGAC CCTTCGGCAG TTTTAAGAAC GATCGTCGAA 
TCGTCTATAG CACTTGGATC AGTAATACAG TCAGATAGAG ACAGGAGATC AATACTCAAC 
AGTCAAGTTG CAAGGGCGTT TAGTAGTTCT ACTGCTGTTG ACGAAGCATC AATATCTCAC 
ATTCCAGTGC TCTCAGGGTT TAGTGTCCCC AAATTAGTTC TACAAGTGGT GTCTGTTGAT 
TCAATTTTCC GGATAACAAG TCCAAGCTTT AATGAGCTTG TCATTCTCAA GGATACTGCT 
TTTTCTGTAT ACAATAAAGC TAGGAGAATT TCACGAGGAA TGCCTAATGA TGCATTTTTC 
TCATCATCTT TACCAAGCAG ATCGTCTTCA GCGTTGACAC CAATGAACTC TATTTCAGGA 
ATCTGGAAAG ACTGCGGTGG TTCTCGAATG ACAGGTTCTA CACATCCAAG AGATGGTGAA 
ATTGATGTTA GTTTGAGAAC GAGTGGTTGG GATACCTCTA CTTCCTGGCA GATACCAAGA 
TCTGGAGGAT TAAGCTGCGA CCCAAACAGA AATGGAGATT TTTACCTTAA TGATGAAATT 
TTCTATTTAT TTGAACCACT TTTCATTCTT TCCGAGCCTG GTTCTGTGGA GCGTGGAGTT 
TCACCTACTT TCACCAGCTT GGGTTCTGAG TCTTCCAAGC CAATACCCGA GGATGGTGGC 
AGAGGTTCTG GACCTGGCAT GAATTCAATG GAAGGTATAA CATCGGGATC AAGCTCCCAG 
GGGGATGTAT CCCAGCTTGA AGGAAAGGCT ATTCCAAGTT TACATTGCTG CTACGGTTGG 
ACAGAGGACT GGAGATGGCT TGTAAGCATC TGGACGGATG CCAGGGGTGA GTTGCTTGAC 
ACACATATAT TTCCCTTTGG TGGAATTAGC AGTAGACAGG ATACAAAAGG GCTGCAGTGT 
CTTTTCGTTC AAGTTCTGCA GCAGGGATGT CAAATACTAC AGGCGTGCTC CTCCCCTGAC 
AATGGATCTT TCAAACCCAG GGATTTTGTC ATTACACGCA TTGGGAATTT CTTCGAGCTT 
GAATACCAAG AGTGGCAAAA GGCAATTTAC TCAGCTGGAG GTCCTGAGAT TAAAAAGTGG 
CCTATTCAAC TGCGACGTTC TGCGCCTTCT GGCATAGCCA CCAACAGCAA CGGATCTTCC 
TTGCAACAGC AGGATCTGAG TCTGATTCAA GAGAGAGCCT CATCAACTAG CACACTCTAC 
AGCTCTCATT CAAAACAATC CACCTTTGTG AAAGGCAGTA TGGGGCAATC CGCTGGAAGA 
AAGCAGATAA TGGGTGGGCA GACCATTTCT GGTACTCCAA GGGGATTGTT TCAGTGGGTT 
CACAGCATCA GTTTTGCTTC TATTTCACTT GACCATTCCC TGCATTTTGT TCTTCCAGCT 
GAGTTGGTAT CCGCTGGTGG TGGCCAGAGC AGTACTGGTA TGAGTTCCGT TAATTACATT 
GAAGGTTTCA CTCCTGTCAA GTCTCTTGGT TCTACCGCTT TCTCTTACAT GATGATACCA 
TCACCCAACA TGCGCTTTCT TCACCCAAGT CCTCTTCAGC TTCCGACATG TTTAACTGCC 
GAGTCACCTC CACTTGCTCA CCTCCTTCAC AGCAAAGGCT ATGCAATCCC CTTGTCTACT 
GGATTTGTTG TTTCAAAAGC TGTGCCTTCC ATGAGAAAAG ACTCAAGGAT AAACGTGAAA 
GAAGAATGGC CATCAGTTCT TTCTGTAAGT CTCATAGACT ATTATGGTGG TTATGACAAC 
GCTCATGACA AAATTCTTCA GGGAATCGTG AAGCAGGGAG GAGGGACCAA AGAAACTAGA 
GATTTTGAGG TTGAAAGCCA TCTTATCCTT GAGTCAATTG CGGCAGAACT CCATGCTCTA 
TCATGGATGA CTGTTAGTCC AGCATATCTG GACAGGCGGA CGGCATTGCC GTTTCACTGT 
GATATGGTTC TGAGATTGAG ACGTCTTCTT CACTTTGCGG ACAAAGAAGT CTCCAGGATA 
CCTGATAAGA CAGGACTGAA AGTTCTGACA ACAGACTCGG GTTCTCAGAG TCTCTCTATG 
TCTCTGCCTC GGGACCATCT TGATGGTATC TGTTTTCAGC ATACAGACTG CAACTTCCTC 
AGGTGTTGCT CCCCTTTCTT ATCTGGACAC ATAAGAAGAG ATGGAGAGCA AGTAAATGGA 
CAGATAAAAA CGGCGGCCAT TTACTACGGC GTTTCTCTCT TTCCAGGCAA AATAGACAAC 
TTAAATGCTA TGGTATGGTC CCATTCTTGT TGCCGGCCAC TTTCTTCTAT GCATCACATG 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185237.1
CDS	215..6220
Translation	MWTNVFRIGGLHNVSWFQFLPSETELNPGFDRSSRAEQNEVATYLVLSSHLRLQKEGFLTTWTNSFVGPWDPSQGLYNPDEKIKLWLFLPGRHSSISDKAQAAVSKLRVVASGIWVAPGDSEEISVAFSQSLRNCIERALSGISYMRFGDVFSKFSPQSEEYLRRGQPTVEFIFAATEEAVFVHVIISAKNVRTLSSGDAERMLRSSLKNSSYRLPVIVSPHGMRGSLTGFCPNDLVKQVYFSSGNLKTSTGYVGLPSHIGRGSRLINGNHCYVEVTLGCCQNRNDNTSQANSTFAVNLPHNQCPEPSVGSKDHRKGQSDLSSVCEKKFIYPAEAVLVPILQSAFAKFSLKRFWLQNWIGPSLAGSSLFMHWAGDFDCLGASENKSDGFYEKNGYNSSGSSRNSSISSTSSASSGSGWRMTSRTGDLDADADSLTCRQSGLTCNDDRLKMGSKRPRTGMAESFGQVGIENDQIGWDWDADDDDDDREVGMDIKALLSEFGDFGDFFENDALPFGEPPGTAESHVLVFPPDSADVGSSPVDMMDVSDQIVLPVGFSSFESFNPVPPIIDECLIKSQEVLHSSITSVPSNQMSISSTGEFDHLLKAEAMMTFAPEYGAVEAPMSEISSTFFKSPYLPKSHKVESSNSRTSNYVYGATPPTTDSDGAGDMILFGSKSCIGNNAGRTLYHSREHYTQVEGRKGRHDKLPTVISDNSSTKEGVSQSIHSKHSAANAVKVVQGKKTDGISAVVSTLLSSKTLLATDVGSVMFQAFMCRMRHIITSSKHSSPVSLTRLSGNFFLNQLSNEPSTLTDNISARNEIYKKDIPTRIAGDFDGGMLDSHMSAPVGVWRTVSVPKTAKPASSPNIEAGSSLPHSSFSEDSLLSYGQRQPLQDLLDGIALLVQQATSFVDLALDSDCGDGPYGWLALEELWRRELSCGPSAGHAGCGGTLASCHSLDIAGVKLVDPLSAEVFPSSVITLLQSDIKTALKSAFGQSDGPLSVTDWCKGRNQSGDGGSISEGFTAESALSEVSNAIDGGKGDETAQSQDIYSSELLRPTLFVLPSPAILVGYQDDWLKISTNSLTHWEKAPFEPYALPKSINYAVVCPDIDPLTCAATDFFQQLGTVYETCRLGTHLPQSLGNQMEKDVGRLSSSGFVLLDCPQSMKIESNNTSLLGSLSDYFLSLSNGWNVNSYLKSLSKALKGLKLGSGLYTNQKEGSGSPCVVVYIVCPFPDPSAVLRTIVESSIALGSVIQSDRDRRSILNSQVARAFSSSTAVDEASISHIPVLSGFSVPKLVLQVVSVDSIFRITSPSFNELVILKDTAFSVYNKARRISRGMPNDAFFSSSLPSRSSSALTPMNSISGIWKDCGGSRMTGSTHPRDGEIDVSLRTSGWDTSTSWQIPRSGGLSCDPNRNGDFYLNDEIFYLFEPLFILSEPGSVERGVSPTFTSLGSESSKPIPEDGGRGSGPGMNSMEGITSGSSSQGDVSQLEGKAIPSLHCCYGWTEDWRWLVSIWTDARGELLDTHIFPFGGISSRQDTKGLQCLFVQVLQQGCQILQACSSPDNGSFKPRDFVITRIGNFFELEYQEWQKAIYSAGGPEIKKWPIQLRRSAPSGIATNSNGSSLQQQDLSLIQERASSTSTLYSSHSKQSTFVKGSMGQSAGRKQIMGGQTISGTPRGLFQWVHSISFASISLDHSLHFVLPAELVSAGGGQSSTGMSSVNYIEGFTPVKSLGSTAFSYMMIPSPNMRFLHPSPLQLPTCLTAESPPLAHLLHSKGYAIPLSTGFVVSKAVPSMRKDSRINVKEEWPSVLSVSLIDYYGGYDNAHDKILQGIVKQGGGTKETRDFEVESHLILESIAAELHALSWMTVSPAYLDRRTALPFHCDMVLRLRRLLHFADKEVSRIPDKTGLKVLTTDSGSQSLSMSLPRDHLDGICFQHTDCNFLRCCSPFLSGHIRRDGEQVNGQIKTAAIYYGVSLFPGKIDNLNAMVWSHSCCRPLSSMHHMA

Target ORF information:

RefSeq Version	NM_001198308.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA polymerase II transcription mediator (GCT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198308.1

ORF Insert Sequence:

ATGTGGACTA ATGTTTTCAG AATTGGTGGT CTACATAATG TTTCTTGGTT TCAGTTTCTT 
CCAAGTGAAA CTGAGCTGAA TCCTGGCTTT GATAGAAGTT CAAGAGCTGA GCAGAATGAA 
GTTGCAACAT ATCTTGTACT TTCCTCTCAT CTACGGTTGC AGAAGGAAGG GTTTCTTACC 
ACTTGGACCA ATTCTTTTGT TGGACCTTGG GATCCATCTC AAGGTTTATA CAACCCTGAT 
GAAAAGATTA AGCTTTGGCT TTTTCTACCT GGGCGTCATT CATCAATATC TGATAAAGCT 
CAGGCTGCTG TGTCAAAGCT TAGAGTTGTT GCGTCAGGAA TTTGGGTAGC ACCTGGGGAC 
TCTGAAGAGA TATCAGTTGC CTTTTCACAG TCTTTACGTA ATTGCATCGA AAGAGCATTA 
TCTGGAATTT CCTACATGAG ATTTGGAGAT GTGTTTTCAA AATTCAGCCC TCAAAGCGAA 
GAATATTTGA GGAGGGGACA GCCTACTGTT GAATTCATCT TTGCTGCTAC CGAAGAGGCA 
GTTTTTGTCC ATGTCATTAT ATCTGCCAAG AATGTCCGCA CACTTTCAAG TGGCGATGCT 
GAAAGAATGT TAAGGAGTTC TTTGAAAAAC TCCAGTTATA GGCTTCCAGT TATTGTTTCT 
CCTCATGGAA TGCGGGGCAG CCTTACTGGA TTCTGTCCCA ATGACCTTGT CAAGCAAGTC 
TACTTCAGTT CTGGAAACTT AAAAACTTCG ACTGGATATG TTGGTCTCCC TTCTCATATT 
GGTCGTGGGT CCCGTCTGAT AAATGGCAAT CATTGCTACG TGGAAGTTAC ACTTGGTTGC 
TGCCAAAATA GAAATGACAA CACAAGTCAA GCTAATTCGA CCTTTGCAGT TAACTTGCCC 
CATAACCAGT GTCCTGAACC TTCAGTTGGG AGTAAAGATC ACCGTAAAGG ACAATCAGAT 
CTTTCATCAG TTTGTGAAAA AAAGTTCATC TATCCAGCTG AGGCAGTGCT TGTACCAATC 
TTACAGTCGG CATTTGCGAA ATTTTCTTTG AAAAGATTTT GGCTTCAAAA CTGGATAGGC 
CCGTCACTAG CAGGCTCATC TTTGTTTATG CACTGGGCTG GTGATTTTGA CTGCCTTGGT 
GCGTCTGAAA ATAAGAGTGA TGGGTTTTAT GAAAAAAATG GTTACAATAG CAGTGGTAGT 
AGCCGTAATA GCAGCATTAG TTCAACAAGT AGCGCTTCCA GCGGGAGCGG CTGGAGGATG 
ACGTCAAGAA CTGGTGATCT TGACGCTGAT GCAGATTCTT TGACGTGTAG GCAATCTGGT 
CTTACTTGTA ATGATGATCG CCTAAAAATG GGTTCCAAGC GGCCGCGAAC AGGGATGGCA 
GAGTCATTCG GTCAAGTAGG CATTGAAAAT GATCAAATTG GTTGGGATTG GGATGCTGAT 
GATGATGATG ATGACAGAGA AGTTGGCATG GATATCAAGG CACTTCTTTC AGAGTTTGGA 
GACTTTGGTG ACTTCTTTGA GAACGATGCT TTACCTTTCG GGGAGCCACC GGGAACTGCA 
GAGTCGCATG TGCTCGTTTT TCCTCCAGAT TCTGCTGATG TAGGTTCCAG TCCCGTGGAT 
ATGATGGATG TATCAGACCA AATCGTTTTG CCTGTTGGGT TTTCCTCTTT TGAGAGCTTT 
AATCCTGTTC CCCCAATCAT TGATGAGTGC CTTATTAAAA GTCAAGAAGT TCTCCACAGC 
AGTATCACTT CAGTTCCTTC AAATCAAATG TCGATCTCTT CGACTGGTGA GTTTGATCAT 
TTACTAAAAG CAGAAGCAAT GATGACCTTT GCTCCTGAGT ATGGAGCTGT AGAAGCACCT 
ATGAGCGAGA TTTCCTCGAC GTTTTTCAAA AGCCCATATC TTCCCAAATC TCATAAAGTG 
GAGAGTTCAA ATTCAAGAAC GAGTAATTAT GTCTATGGAG CCACACCACC GACCACTGAT 
TCTGATGGAG CAGGCGATAT GATTTTGTTC GGATCAAAAT CATGCATTGG AAACAATGCT 
GGCAGAACTC TGTATCATTC GAGGGAGCAT TACACTCAAG TAGAAGGTAG AAAGGGCCGA 
CATGATAAGC TACCTACAGT TATCAGTGAC AACAGTAGTA CAAAGGAGGG TGTCTCTCAA 
TCAATACACT CAAAACATAG TGCTGCGAAT GCTGTTAAGG TCGTACAGGG AAAAAAAACT 
GATGGTATAT CTGCTGTTGT TAGTACCTTA TTATCTTCAA AGACCCTGCT GGCAACAGAC 
GTGGGAAGTG TTATGTTCCA AGCTTTCATG TGTAGGATGC GGCACATAAT CACTTCTTCA 
AAGCACAGTT CACCTGTTAG TCTGACTAGG CTTAGCGGAA ACTTTTTCCT TAACCAGCTG 
TCAAATGAGC CTAGTACTCT GACAGACAAC ATATCTGCAA GGAACGAGAT ATATAAGAAA 
GATATACCTA CCAGAATAGC TGGAGATTTT GACGGAGGAA TGTTGGATAG CCACATGAGT 
GCACCAGTTG GCGTATGGCG GACTGTTTCA GTCCCAAAAA CAGCAAAACC TGCTAGTTCG 
CCAAATATTG AAGCAGGGTC ATCCTTGCCC CATAGTTCAT TTAGCGAAGA CAGCTTACTT 
TCTTATGGTC AAAGGCAACC ACTGCAGGAT CTCCTAGATG GAATAGCGCT ACTTGTACAG 
CAAGCTACCT CTTTTGTTGA TTTAGCTCTG GATTCAGATT GTGGAGATGG ACCTTATGGC 
TGGCTTGCAT TGGAAGAGTT ATGGAGACGG GAATTGTCGT GTGGTCCCTC TGCTGGCCAT 
GCAGGTTGTG GGGGAACTTT GGCTTCCTGC CATTCTCTGG ACATTGCTGG TGTCAAGCTT 
GTTGATCCAC TCTCTGCTGA GGTTTTTCCT TCGTCTGTCA TTACTCTTCT GCAATCTGAC 
ATCAAAACAG CTCTGAAATC TGCATTTGGT CAGTCAGATG GTCCATTATC TGTTACAGAT 
TGGTGCAAGG GCCGAAATCA ATCGGGGGAT GGAGGATCTA TTTCTGAGGG ATTTACTGCT 
GAGTCAGCCC TGAGTGAAGT CTCAAATGCA ATAGATGGTG GGAAAGGAGA TGAGACGGCT 
CAGAGCCAAG ACATATACAG TTCAGAATTA CTCCGACCGA CACTTTTTGT TCTTCCATCA 
CCTGCTATAC TTGTCGGGTA CCAAGATGAC TGGCTTAAGA TATCAACAAA CTCCTTGACA 
CATTGGGAAA AGGCCCCCTT TGAGCCATAT GCTCTGCCAA AAAGTATTAA TTATGCTGTT 
GTATGCCCGG ATATTGATCC TTTAACTTGT GCTGCTACTG ATTTCTTCCA GCAGCTTGGA 
ACTGTTTATG AAACGTGCAG GCTGGGAACA CATTTGCCAC AGAGCCTGGG AAATCAAATG 
GAAAAGGACG TTGGGAGATT ATCCTCATCT GGATTTGTTC TGCTTGATTG CCCTCAATCA 
ATGAAGATTG AAAGCAATAA CACATCGCTT CTGGGATCTC TCAGCGATTA TTTTCTATCT 
TTATCAAATG GGTGGAATGT GAATAGCTAT CTGAAGTCTT TATCAAAAGC GCTGAAAGGT 
CTAAAGCTTG GATCAGGCCT TTACACAAAC CAAAAAGAAG GATCAGGAAG TCCTTGTGTG 
GTAGTGTACA TAGTGTGTCC ATTTCCTGAC CCTTCGGCAG TTTTAAGAAC GATCGTCGAA 
TCGTCTATAG CACTTGGATC AGTAATACAG TCAGATAGAG ACAGGAGATC AATACTCAAC 
AGTCAAGTTG CAAGGGCGTT TAGTAGTTCT ACTGCTGTTG ACGAAGCATC AATATCTCAC 
ATTCCAGTGC TCTCAGGGTT TAGTGTCCCC AAATTAGTTC TACAAGTGGT GTCTGTTGAT 
TCAATTTTCC GGATAACAAG TCCAAGCTTT AATGAGCTTG TCATTCTCAA GGATACTGCT 
TTTTCTGTAT ACAATAAAGC TAGGAGAATT TCACGAGGAA TGCCTAATGA TGCATTTTTC 
TCATCATCTT TACCAAGCAG ATCGTCTTCA GCGTTGACAC CAATGAACTC TATTTCAGGA 
ATCTGGAAAG ACTGCGGTGG TTCTCGAATG ACAGGTTCTA CACATCCAAG AGATGGTGAA 
ATTGATGTTA GTTTGAGAAC GAGTGGTTGG GATACCTCTA CTTCCTGGCA GATACCAAGA 
TCTGGAGGAT TAAGCTGCGA CCCAAACAGA AATGGAGATT TTTACCTTAA TGATGAAATT 
TTCTATTTAT TTGAACCACT TTTCATTCTT TCCGAGCCTG GTTCTGTGGA GCGTGGAGTT 
TCACCTACTT TCACCAGCTT GGGTTCTGAG TCTTCCAAGC CAATACCCGA GGATGGTGGC 
AGAGGTTCTG GACCTGGCAT GAATTCAATG GAAGGTATAA CATCGGGATC AAGCTCCCAG 
GGGGATGTAT CCCAGCTTGA AGGAAAGGCT ATTCCAAGTT TACATTGCTG CTACGGTTGG 
ACAGAGGACT GGAGATGGCT TGTAAGCATC TGGACGGATG CCAGGGGTGA GTTGCTTGAC 
ACACATATAT TTCCCTTTGG TGGAATTAGC AGTAGACAGG ATACAAAAGG GCTGCAGTGT 
CTTTTCGTTC AAGTTCTGCA GCAGGGATGT CAAATACTAC AGGCGTGCTC CTCCCCTGAC 
AATGGATCTT TCAAACCCAG GGATTTTGTC ATTACACGCA TTGGGAATTT CTTCGAGCTT 
GAATACCAAG AGTGGCAAAA GGCAATTTAC TCAGCTGGAG GTCCTGAGAT TAAAAAGTGG 
CCTATTCAAC TGCGACGTTC TGCGCCTTCT GGCATAGCCA CCAACAGCAA CGGATCTTCC 
TTGCAACAGC AGGATCTGAG TCTGATTCAA GAGAGAGCCT CATCAACTAG CACACTCTAC 
AGCTCTCATT CAAAACAATC CACCTTTGTG AAAGGCAGTA TGGGGCAATC CGCTGGAAGA 
AAGCAGATAA TGGGTGGGCA GACCATTTCT GGTACTCCAA GGGGATTGTT TCAGTGGGTT 
CACAGCATCA GTTTTGCTTC TATTTCACTT GACCATTCCC TGCATTTTGT TCTTCCAGCT 
GAGTTGGTAT CCGCTGGTGG TGGCCAGAGC AGTACTGGTA TGAGTTCCGT TAATTACATT 
GAAGGTTTCA CTCCTGTCAA GTCTCTTGGT TCTACCGCTT TCTCTTACAT GATGATACCA 
TCACCCAACA TGCGCTTTCT TCACCCAAGT CCTCTTCAGC TTCCGACATG TTTAACTGCC 
GAGTCACCTC CACTTGCTCA CCTCCTTCAC AGCAAAGGCT ATGCAATCCC CTTGTCTACT 
GGATTTGTTG TTTCAAAAGC TGTGCCTTCC ATGAGAAAAG ACTCAAGGAT AAACGTGAAA 
GAAGAATGGC CATCAGTTCT TTCTGTAAGT CTCATAGACT ATTATGGTGG TTATGACAAC 
GCTCATGACA AAATTCTTCA GGGAATCGTG AAGCAGGGAG GAGGGACCAA AGAAACTAGA 
GATTTTGAGG TTGAAAGCCA TCTTATCCTT GAGTCAATTG CGGCAGAACT CCATGCTCTA 
TCATGGATGA CTGTTAGTCC AGCATATCTG GACAGGCGGA CGGCATTGCC GTTTCACTGT 
GATATGGTTC TGAGATTGAG ACGTCTTCTT CACTTTGCGG ACAAAGAAGT CTCCAGGATA 
CCTGATAAGA CAGGACTGAA AGTTCTGACA ACAGACTCGG GTTCTCAGAG TCTCTCTATG 
TCTCTGCCTC GGGACCATCT TGATGGTATC TGTTTTCAGC ATACAGACTG CAACTTCCTC 
AGGTGTTGCT CCCCTTTCTT ATCTGGACAC ATAAGAAGAG ATGGAGAGCA AGTAAATGGA 
CAGATAAAAA CGGCGGCCAT TTACTACGGC GTTTCTCTCT TTCCAGGCAA AATAGACAAC 
TTAAATGCTA TGGTATGGTC CCATTCTTGT TGCCGGCCAC TTTCTTCTAT GCATCACATG 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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GCT cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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