MNS3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following MNS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MNS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb30409 NM_102740.3
Latest version!
Arabidopsis thaliana alpha-mannosidase 3 (MNS3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OAb33968 NM_001332879.1
Latest version!
Arabidopsis thaliana alpha-mannosidase 3 (MNS3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb30409
    Clone ID Related Accession (Same CDS sequence) NM_102740.3
    Accession Version NM_102740.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product alpha-mannosidase 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_102740.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGAAAT CTCTACCATA TTCAGTTAAA GATATTCATT ACGACAATGC CAAGTTCAGA 
    CATCGATCTC CTTTAAAGGT TTTTTCTCAA AGCTTGCTTA CTCTTAGTAC AAAACGCAAC
    TACGCTAGCT GTAGCACGGG GAAGTTCCTT ATATTAATAC TGTTCTTTGG AGTAGCATGT
    CTAATGCTGA TGAGTAAGAG TCCAAATGAA TCTGGTCTTA ATGAGAAAGG AAAGGTCACT
    TTTGTTGGGG GACTTAGGCT AGGGGGACTT TTGAGAAAGC CACCTAGGCT TCCACCTCGT
    TTATCACCTG ACGAAGGACA ATTAAGAGGA AGTTCCACTA ATGGCTCAAC TATTTCAAAT
    TCTGACCCAA AATGGGCAGC TAGGCAGCAA AGTGTAAAAG AGGCTTTTGA CCATGCATGG
    TCTGGCTATA GAAAGTACGC CATGGGTTAT GACGAGCTAA TGCCTATTAG CCAGAAAGGT
    GTGGATGGTT TAGGAGGATT AGGTGCCACT GTTGTGGATG CTCTTGATAC AGCCATGATA
    ATGGGTCTTG ATAATATCGT CTCTGAAGCA GGATCATGGG TTGAGACTCA TCTCTTGGAG
    AGAATCAGTC AAAAAGGTCA AGTTAATTTG TTTGAAACTA CAATACGTGT CTTAGGTGGG
    CTTCTAAGTG CATACCATTT GAGTGGAGGA GAGCAGGGTA CGGTGAACAT GACCCATGTT
    GGACCGAAAC CAGTCATCTA TCTAAATATT GCTAAGGATT TGGCTGACCG TCTACTTTCG
    GCTTTTACCT CCAGCCCTAC TCCAGTTCCC TTTTGTGATG TCATATTGCA TGAATCGACT
    GCTCATCCAG CTCCAGGAGG AGCGAGCAGT ACAGCAGAAG TTGCCTCTGT ACAGCTTGAA
    TTTAACTACC TCAGTTCTAT TTCTGGTGAT CCAAAGTATA GTACAGAGGC CATGAAGGTC
    TTAGCTCATA TAAAAACACT TCCAAAGACA GAAGGTCTTG TTCCAATCTA TATCAGCCCT
    CAAACAGGTG ACTTTGTTGG TGAGAATATC AGGTTGGGAT CTCGTGGTGA TAGCTACTAT
    GAGTATTTAA TTAAGGTGTG GCTTCAGCAA GGAGCTAAGT TGAATAGTAA TTTCACATAT
    TTGCATGATA TGTACATTGA GGCAATGAAA GGAGTCAGGC ATTTGCTTGT GCAAAATTCA
    ATTCCAAAAG GTCTGGTCTT TGTAGGGGAG TTACCTTACG GGTCTAAGGG AGAATTCAGT
    CCAAAAATGG ATCATTTGGT ATGCTTCTTG CCTGGTACTC TTGCTCTCGG TGCTACCAAG
    GGCCTCACAA AGGAACAAGC CCTGAAGGAA AATCTTTTGT CCTTTGAGGA CCTAGAAAAT
    CTGAAACTTG CAGAAGATTT AGCGAAAACA TGCTTTGAGA TGTATGAAGT AACTGCCACT
    GGCCTTGCTC CAGAAATTGC TTACTTTCAC ACAAAGGATT ACACTGAGGA TGGTCTTGAT
    GGTGGGAATA AGAGTTCTAT GTATGCAAAT GACATAATCA TTAAGCCCGC TGATCGACAT
    AATCTACTGC GCCCTGAAAC TGTTGAATCA CTCTTTGTCC TCTATCGTAT CACAAAAGAT
    ACAAAATACC GAGATCAAGG TTGGCAAATT TTTGAAGCTT TTGAAAAGTA CACAAAGGTG
    AAATCAGGTG GATACACATC ATTGGATGAT GTAACAGAAG TGCCTCCTCA TAGAAGAGAT
    AAGATGGAGA CATTTTTCCT TGGTGAAACA TTGAAGTACT TATACTTGCT CTTTGGAGAT
    GACTCAGTGA TACCATTGGA TAAGTTTGTT TTCAACACTG AAGCTCATCC ATTACCAATA
    AGAAGAAACA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_564345.1
    CDS239..2113
    Translation

    Target ORF information:

    RefSeq Version NM_102740.3
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana alpha-mannosidase 3 (MNS3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_102740.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGAAAT CTCTACCATA TTCAGTTAAA GATATTCATT ACGACAATGC CAAGTTCAGA 
    CATCGATCTC CTTTAAAGGT TTTTTCTCAA AGCTTGCTTA CTCTTAGTAC AAAACGCAAC
    TACGCTAGCT GTAGCACGGG GAAGTTCCTT ATATTAATAC TGTTCTTTGG AGTAGCATGT
    CTAATGCTGA TGAGTAAGAG TCCAAATGAA TCTGGTCTTA ATGAGAAAGG AAAGGTCACT
    TTTGTTGGGG GACTTAGGCT AGGGGGACTT TTGAGAAAGC CACCTAGGCT TCCACCTCGT
    TTATCACCTG ACGAAGGACA ATTAAGAGGA AGTTCCACTA ATGGCTCAAC TATTTCAAAT
    TCTGACCCAA AATGGGCAGC TAGGCAGCAA AGTGTAAAAG AGGCTTTTGA CCATGCATGG
    TCTGGCTATA GAAAGTACGC CATGGGTTAT GACGAGCTAA TGCCTATTAG CCAGAAAGGT
    GTGGATGGTT TAGGAGGATT AGGTGCCACT GTTGTGGATG CTCTTGATAC AGCCATGATA
    ATGGGTCTTG ATAATATCGT CTCTGAAGCA GGATCATGGG TTGAGACTCA TCTCTTGGAG
    AGAATCAGTC AAAAAGGTCA AGTTAATTTG TTTGAAACTA CAATACGTGT CTTAGGTGGG
    CTTCTAAGTG CATACCATTT GAGTGGAGGA GAGCAGGGTA CGGTGAACAT GACCCATGTT
    GGACCGAAAC CAGTCATCTA TCTAAATATT GCTAAGGATT TGGCTGACCG TCTACTTTCG
    GCTTTTACCT CCAGCCCTAC TCCAGTTCCC TTTTGTGATG TCATATTGCA TGAATCGACT
    GCTCATCCAG CTCCAGGAGG AGCGAGCAGT ACAGCAGAAG TTGCCTCTGT ACAGCTTGAA
    TTTAACTACC TCAGTTCTAT TTCTGGTGAT CCAAAGTATA GTACAGAGGC CATGAAGGTC
    TTAGCTCATA TAAAAACACT TCCAAAGACA GAAGGTCTTG TTCCAATCTA TATCAGCCCT
    CAAACAGGTG ACTTTGTTGG TGAGAATATC AGGTTGGGAT CTCGTGGTGA TAGCTACTAT
    GAGTATTTAA TTAAGGTGTG GCTTCAGCAA GGAGCTAAGT TGAATAGTAA TTTCACATAT
    TTGCATGATA TGTACATTGA GGCAATGAAA GGAGTCAGGC ATTTGCTTGT GCAAAATTCA
    ATTCCAAAAG GTCTGGTCTT TGTAGGGGAG TTACCTTACG GGTCTAAGGG AGAATTCAGT
    CCAAAAATGG ATCATTTGGT ATGCTTCTTG CCTGGTACTC TTGCTCTCGG TGCTACCAAG
    GGCCTCACAA AGGAACAAGC CCTGAAGGAA AATCTTTTGT CCTTTGAGGA CCTAGAAAAT
    CTGAAACTTG CAGAAGATTT AGCGAAAACA TGCTTTGAGA TGTATGAAGT AACTGCCACT
    GGCCTTGCTC CAGAAATTGC TTACTTTCAC ACAAAGGATT ACACTGAGGA TGGTCTTGAT
    GGTGGGAATA AGAGTTCTAT GTATGCAAAT GACATAATCA TTAAGCCCGC TGATCGACAT
    AATCTACTGC GCCCTGAAAC TGTTGAATCA CTCTTTGTCC TCTATCGTAT CACAAAAGAT
    ACAAAATACC GAGATCAAGG TTGGCAAATT TTTGAAGCTT TTGAAAAGTA CACAAAGGTG
    AAATCAGGTG GATACACATC ATTGGATGAT GTAACAGAAG TGCCTCCTCA TAGAAGAGAT
    AAGATGGAGA CATTTTTCCT TGGTGAAACA TTGAAGTACT TATACTTGCT CTTTGGAGAT
    GACTCAGTGA TACCATTGGA TAAGTTTGTT TTCAACACTG AAGCTCATCC ATTACCAATA
    AGAAGAAACA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb33968
    Clone ID Related Accession (Same CDS sequence) NM_001332879.1
    Accession Version NM_001332879.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product alpha-mannosidase 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGTTATG CCCAAGTTTT CTCCTTTATT ATGGATTTGA TGTCACTGGT TCTTGTATCT 
    GGTTCATCTT CTTTGGTTTT AGATGAATTG CTTTGTGTCC AAATTGACCT TGGATTCACG
    AAATTACGTT TTTGTGAGGT TTTTTCTCAA AGCTTGCTTA CTCTTAGTAC AAAACGCAAC
    TACGCTAGCT GTAGCACGGG GAAGTTCCTT ATATTAATAC TGTTCTTTGG AGTAGCATGT
    CTAATGCTGA TGAGTAAGAG TCCAAATGAA TCTGGTCTTA ATGAGAAAGG AAAGGTCACT
    TTTGTTGGGG GACTTAGGCT AGGGGGACTT TTGAGAAAGC CACCTAGGCT TCCACCTCGT
    TTATCACCTG ACGAAGGACA ATTAAGAGGA AGTTCCACTA ATGGCTCAAC TATTTCAAAT
    TCTGACCCAA AATGGGCAGC TAGGCAGCAA AGTGTAAAAG AGGCTTTTGA CCATGCATGG
    TCTGGCTATA GAAAGTACGC CATGGGTTAT GACGAGCTAA TGCCTATTAG CCAGAAAGGT
    GTGGATGGTT TAGGAGGATT AGGTGCCACT GTTGTGGATG CTCTTGATAC AGCCATGATA
    ATGGGTCTTG ATAATATCGT CTCTGAAGCA GGATCATGGG TTGAGACTCA TCTCTTGGAG
    AGAATCAGTC AAAAAGGTCA AGTTAATTTG TTTGAAACTA CAATACGTGT CTTAGGTGGG
    CTTCTAAGTG CATACCATTT GAGTGGAGGA GAGCAGGGTA CGGTGAACAT GACCCATGTT
    GGACCGAAAC CAGTCATCTA TCTAAATATT GCTAAGGATT TGGCTGACCG TCTACTTTCG
    GCTTTTACCT CCAGCCCTAC TCCAGTTCCC TTTTGTGATG TCATATTGCA TGAATCGACT
    GCTCATCCAG CTCCAGGAGG AGCGAGCAGT ACAGCAGAAG TTGCCTCTGT ACAGCTTGAA
    TTTAACTACC TCAGTTCTAT TTCTGGTGAT CCAAAGTATA GTACAGAGGC CATGAAGGTC
    TTAGCTCATA TAAAAACACT TCCAAAGACA GAAGGTCTTG TTCCAATCTA TATCAGCCCT
    CAAACAGGTG ACTTTGTTGG TGAGAATATC AGGTTGGGAT CTCGTGGTGA TAGCTACTAT
    GAGTATTTAA TTAAGGTGTG GCTTCAGCAA GGAGCTAAGT TGAATAGTAA TTTCACATAT
    TTGCATGATA TGTACATTGA GGCAATGAAA GGAGTCAGGC ATTTGCTTGT GCAAAATTCA
    ATTCCAAAAG GTCTGGTCTT TGTAGGGGAG TTACCTTACG GGTCTAAGGG AGAATTCAGT
    CCAAAAATGG ATCATTTGGT ATGCTTCTTG CCTGGTACTC TTGCTCTCGG TGCTACCAAG
    GGCCTCACAA AGGAACAAGC CCTGAAGGAA AATCTTTTGT CCTTTGAGGA CCTAGAAAAT
    CTGAAACTTG CAGAAGATTT AGCGAAAACA TGCTTTGAGA TGTATGAAGT AACTGCCACT
    GGCCTTGCTC CAGAAATTGC TTACTTTCAC ACAAAGGATT ACACTGAGGA TGGTCTTGAT
    GGTGGGAATA AGAGTTCTAT GTATGCAAAT GACATAATCA TTAAGCCCGC TGATCGACAT
    AATCTACTGC GCCCTGAAAC TGTTGAATCA CTCTTTGTCC TCTATCGTAT CACAAAAGAT
    ACAAAATACC GAGATCAAGG TTGGCAAATT TTTGAAGCTT TTGAAAAGTA CACAAAGGTG
    AAATCAGGTG GATACACATC ATTGGATGAT GTAACAGAAG TGCCTCCTCA TAGAAGAGAT
    AAGATGGAGA CATTTTTCCT TGGTGAAACA TTGAAGTACT TATACTTGCT CTTTGGAGAT
    GACTCAGTGA TACCATTGGA TAAGTTTGTT TTCAACACTG AAGCTCATCC ATTACCAATA
    AGAAGAAACA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001320239.1
    CDS337..2271
    Translation

    Target ORF information:

    RefSeq Version NM_001332879.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana alpha-mannosidase 3 (MNS3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001332879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGTTATG CCCAAGTTTT CTCCTTTATT ATGGATTTGA TGTCACTGGT TCTTGTATCT 
    GGTTCATCTT CTTTGGTTTT AGATGAATTG CTTTGTGTCC AAATTGACCT TGGATTCACG
    AAATTACGTT TTTGTGAGGT TTTTTCTCAA AGCTTGCTTA CTCTTAGTAC AAAACGCAAC
    TACGCTAGCT GTAGCACGGG GAAGTTCCTT ATATTAATAC TGTTCTTTGG AGTAGCATGT
    CTAATGCTGA TGAGTAAGAG TCCAAATGAA TCTGGTCTTA ATGAGAAAGG AAAGGTCACT
    TTTGTTGGGG GACTTAGGCT AGGGGGACTT TTGAGAAAGC CACCTAGGCT TCCACCTCGT
    TTATCACCTG ACGAAGGACA ATTAAGAGGA AGTTCCACTA ATGGCTCAAC TATTTCAAAT
    TCTGACCCAA AATGGGCAGC TAGGCAGCAA AGTGTAAAAG AGGCTTTTGA CCATGCATGG
    TCTGGCTATA GAAAGTACGC CATGGGTTAT GACGAGCTAA TGCCTATTAG CCAGAAAGGT
    GTGGATGGTT TAGGAGGATT AGGTGCCACT GTTGTGGATG CTCTTGATAC AGCCATGATA
    ATGGGTCTTG ATAATATCGT CTCTGAAGCA GGATCATGGG TTGAGACTCA TCTCTTGGAG
    AGAATCAGTC AAAAAGGTCA AGTTAATTTG TTTGAAACTA CAATACGTGT CTTAGGTGGG
    CTTCTAAGTG CATACCATTT GAGTGGAGGA GAGCAGGGTA CGGTGAACAT GACCCATGTT
    GGACCGAAAC CAGTCATCTA TCTAAATATT GCTAAGGATT TGGCTGACCG TCTACTTTCG
    GCTTTTACCT CCAGCCCTAC TCCAGTTCCC TTTTGTGATG TCATATTGCA TGAATCGACT
    GCTCATCCAG CTCCAGGAGG AGCGAGCAGT ACAGCAGAAG TTGCCTCTGT ACAGCTTGAA
    TTTAACTACC TCAGTTCTAT TTCTGGTGAT CCAAAGTATA GTACAGAGGC CATGAAGGTC
    TTAGCTCATA TAAAAACACT TCCAAAGACA GAAGGTCTTG TTCCAATCTA TATCAGCCCT
    CAAACAGGTG ACTTTGTTGG TGAGAATATC AGGTTGGGAT CTCGTGGTGA TAGCTACTAT
    GAGTATTTAA TTAAGGTGTG GCTTCAGCAA GGAGCTAAGT TGAATAGTAA TTTCACATAT
    TTGCATGATA TGTACATTGA GGCAATGAAA GGAGTCAGGC ATTTGCTTGT GCAAAATTCA
    ATTCCAAAAG GTCTGGTCTT TGTAGGGGAG TTACCTTACG GGTCTAAGGG AGAATTCAGT
    CCAAAAATGG ATCATTTGGT ATGCTTCTTG CCTGGTACTC TTGCTCTCGG TGCTACCAAG
    GGCCTCACAA AGGAACAAGC CCTGAAGGAA AATCTTTTGT CCTTTGAGGA CCTAGAAAAT
    CTGAAACTTG CAGAAGATTT AGCGAAAACA TGCTTTGAGA TGTATGAAGT AACTGCCACT
    GGCCTTGCTC CAGAAATTGC TTACTTTCAC ACAAAGGATT ACACTGAGGA TGGTCTTGAT
    GGTGGGAATA AGAGTTCTAT GTATGCAAAT GACATAATCA TTAAGCCCGC TGATCGACAT
    AATCTACTGC GCCCTGAAAC TGTTGAATCA CTCTTTGTCC TCTATCGTAT CACAAAAGAT
    ACAAAATACC GAGATCAAGG TTGGCAAATT TTTGAAGCTT TTGAAAAGTA CACAAAGGTG
    AAATCAGGTG GATACACATC ATTGGATGAT GTAACAGAAG TGCCTCCTCA TAGAAGAGAT
    AAGATGGAGA CATTTTTCCT TGGTGAAACA TTGAAGTACT TATACTTGCT CTTTGGAGAT
    GACTCAGTGA TACCATTGGA TAAGTTTGTT TTCAACACTG AAGCTCATCC ATTACCAATA
    AGAAGAAACA CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
    Nature408(6814)816-20(2000 Dec)
    Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW