POLH cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLH
Entrez Gene ID	834503
Full Name	Y-family DNA polymerase H
Synonyms	K23L20.8,K23L20_8,Y-family DNA polymerase H
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001344572.1	NP_001332153.1	Y-family DNA polymerase H
NM_180799.2	NP_851130.1	Y-family DNA polymerase H
NM_123841.5	NP_568638.2	Y-family DNA polymerase H

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	POLH	NP_568638.2
Oryza sativa (rice)	Os01g0757800	NP_001044297.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cooperation among DNA translesion synthesis (TLS) polymerases (Poleta, Polzeta) and DNA-damage-activated protein kinases (ATR, ATM)
Title: Cooperative responses of DNA-damage-activated protein kinases ATR and ATM and DNA translesion polymerases to replication-blocking DNA damage in a stem-cell niche.

The mutation frequency in AtPolzeta-, AtRev1- or AtPoleta-knockout plants rev3-1, rev1-1 and polh-1, respectively, were analyzed.
Title: Role of AtPol?, AtRev1 and AtPol? in ? ray-induced mutagenesis.

Arabidopsis thaliana disruptants of AtREV3, AtREV1, and/or AtPOLH genes that encode Translesion synthesis-type polymerases.
Title: Role of AtPol?, AtRev1, and AtPol? in UV light-induced mutagenesis in Arabidopsis.

Results showed that crossing two lines homozygous for insertions in AtREV3 and AtPOLH and selfing F1 plants yielded non-Mendelian F2 genotype distributions: frequencies of +/++/+ and 1/1 2/2 progeny were only 0.42 and 0.25%.
Title: Reciprocal chromosome translocation associated with TDNA-insertion mutation in Arabidopsis: genetic and cytological analyses of consequences for gametophyte development and for construction of doubly mutant lines.

AtPolH confers UV resistance by interacting with PNCA2 and catalyzing translesion synthesis. PCNA1 does not interact with AtPolH to restore UV resistance in deficient mutants.
Title: Arabidopsis thaliana Y-family DNA polymerase eta catalyses translesion synthesis and interacts functionally with PCNA2.

The following POLH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb20555	NM_180799.2 Latest version!	Arabidopsis thaliana Y-family DNA polymerase H (POLH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb20556	NM_123841.5 Latest version!	Arabidopsis thaliana Y-family DNA polymerase H (POLH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb41595	NM_001344572.1 Latest version!	Arabidopsis thaliana Y-family DNA polymerase H (POLH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLH ( NM_180799.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_851130.1 Arabidopsis thaliana Y-family DNA polymerase H (POLH), mRNA.

Price & Availability↑

CloneID	OAb20555
Clone ID Related Accession (Same CDS sequence)	NM_180799.2
Accession Version	NM_180799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Y-family DNA polymerase H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_180799.1.

ATGAGGCTAA AGCTGCTTGT CCTCAGATTC AATTGGTTCA AGTTCCTGTG GCTCGTGGTG 
GTTTCGATTC TAGCAAAAAG CGGAAAATGT GAGAGGGCTT CGATTGATGA AGTGTATCTT 
GACCTCACTG ATGCAGCAGA AAGCATGCTT GCTGATGCAC CTCCAGAGAG TTTGGAATTG 
ATAGATGAGG AAGTTCTTAA ATCCCATATT CTTGGAATGA ACAGAGAGGA TGGAGATGAT 
TTCAAGGAGA GTGTCCGCAA TTGGATTTGT CGAGAAGATG CTGATCGACG TGATAAGTTA 
TTAAGTTGTG GAATTATCAT TGTTGCAGAA CTCCGGAAAC AGGTGCTTAA GGAGACTGAG 
TTTACTTGTT CTGCTGGCAT AGCCCATAAC AAGATGCTAG CCAAACTTGC TAGTGGAATG 
AACAAACCTG CGCAGCAGAC AGTTGTACCA TATGCAGCTG TACAGGAATT ACTAAGTTCC 
TTGCCAATAA AGAAAATGAA ACAACTGGGA GGAAAGCTGG GCACTAGCTT GCAAACTGAC 
TTGGGAGTTG ACACAGTAGG GGACTTGCTG CAATTTTCTG AAACGAAGCT ACAAGAACAT 
TACGGAGTAA ATACTGGTAC TTGGTTATGG AATATAGCAA GAGGGATCAG TGGAGAAGAA 
GTGCAAGGTC GCCTTCTCCC CAAAAGTCAT GGTTCTGGAA AGACATTTCC CGGACCTCGT 
GCTTTGAAGT CACTCTCAAC TGTTCAGCAT TGGCTGAATC AGCTTTCCGA GGAACTAAGC 
GAGCGCCTCG GTTCTGACTT GGAGCAGAAT AAGCGGATTG CAAGCACCCT TACCCTTCAT 
GCTAGCGCTT TCAGATCAAA GGATTCAGAT TCTCATAAGA AATTTCCTTC AAAATCATGT 
CCTATGAGAT ATGGAGTAAC CAAGATCCAA GAAGATGCCT TTAACTTGTT CCAAGCTGCC 
TTACGTGAGT ACATGGGATC CTTCGGGATC AAACCTCAAG GAAATAAGCT AGAGACATGG 
AGGATAACAG GTCTTTCTGT TTCAGCGAGT AAAATTGTTG ATATACCATC TGGAACAAGC 
TCGATCATGA GATATTTTCA AAGTCAACCT ACTGTTCCTT CTCGTTCAGC AGATGGTTGT 
GTTCAAGGAA ATGTAGCGAT GACTGCTTCA GCTAGCGAAG GATGTTCAGA ACAGAGGTCA 
ACAGAAACTC AAGCAGCAAT GCCTGAAGTA GACACCGGTG TAACTTATAC TTTGCCTAAT 
TTTGAAAACC AAGATAAAGA CATTGATCTA GTCTCCGAAA AGGATGTTGT CTCTTGTCCA 
TCAAACGAAG CAACGGATGT TTCTACCCAA TCAGAATCAA ACAAAGGCAC ACAGACAAAG 
AAAATTGGGC GGAAGATGAA CAACTCGAAA GAAAAGAATC GGGGAATGCC TTCAATTGTC 
GATATCTTTA AGAATTACAA TGCAACACCT CCATCAAAAC AAGAGACTCA AGAAGATTCC 
ACAGTGTCAT CAGCCAGTAA AAGAGCAAAG TTAAGTAGCA GCAGCCATAA TAGTCAAGTG 
AATCAAGAGG TAGAAGAGAG TAGAGAAACA GATTGGGGAT ATAAGACTGA TGAAATCGAT 
CAGTCAGTTT TCGATGAATT ACCGGTTGAG ATTCAACGAG AACTCAGGAG TTTCCTACGC 
ACCAATAAGC AGTTTAACAC CGGTAAAAGC AAAGGTGATG GTTCTACTTC AAGCATTGCT 
CATTATTTTC CACCATTGAA TAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851130.1
CDS	315..2081
Translation	MRLKLLVLRFNWFKFLWLVVVSILAKSGKCERASIDEVYLDLTDAAESMLADAPPESLELIDEEVLKSHILGMNREDGDDFKESVRNWICREDADRRDKLLSCGIIIVAELRKQVLKETEFTCSAGIAHNKMLAKLASGMNKPAQQTVVPYAAVQELLSSLPIKKMKQLGGKLGTSLQTDLGVDTVGDLLQFSETKLQEHYGVNTGTWLWNIARGISGEEVQGRLLPKSHGSGKTFPGPRALKSLSTVQHWLNQLSEELSERLGSDLEQNKRIASTLTLHASAFRSKDSDSHKKFPSKSCPMRYGVTKIQEDAFNLFQAALREYMGSFGIKPQGNKLETWRITGLSVSASKIVDIPSGTSSIMRYFQSQPTVPSRSADGCVQGNVAMTASASEGCSEQRSTETQAAMPEVDTGVTYTLPNFENQDKDIDLVSEKDVVSCPSNEATDVSTQSESNKGTQTKKIGRKMNNSKEKNRGMPSIVDIFKNYNATPPSKQETQEDSTVSSASKRAKLSSSSHNSQVNQEVEESRETDWGYKTDEIDQSVFDELPVEIQRELRSFLRTNKQFNTGKSKGDGSTSSIAHYFPPLNR

Target ORF information:

RefSeq Version	NM_180799.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Y-family DNA polymerase H (POLH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180799.2

ORF Insert Sequence:

ATGAGGCTAA AGCTGCTTGT CCTCAGATTC AATTGGTTCA AGTTCCTGTG GCTCGTGGTG 
GTTTCGATTC TAGCAAAAAG CGGAAAATGT GAGAGGGCTT CGATTGATGA AGTGTATCTT 
GACCTCACTG ATGCAGCAGA AAGCATGCTT GCTGATGCAC CTCCAGAGAG TTTGGAATTG 
ATAGATGAGG AAGTTCTTAA ATCCCATATT CTTGGAATGA ACAGAGAGGA TGGAGATGAT 
TTCAAGGAGA GTGTCCGCAA TTGGATTTGT CGAGAAGATG CTGATCGACG TGATAAGTTA 
TTAAGTTGTG GAATTATCAT TGTTGCAGAA CTCCGGAAAC AGGTGCTTAA GGAGACTGAG 
TTTACTTGTT CTGCTGGCAT AGCCCATAAC AAGATGCTAG CCAAACTTGC TAGTGGAATG 
AACAAACCTG CGCAGCAGAC AGTTGTACCA TATGCAGCTG TACAGGAATT ACTAAGTTCC 
TTGCCAATAA AGAAAATGAA ACAACTGGGA GGAAAGCTGG GCACTAGCTT GCAAACTGAC 
TTGGGAGTTG ACACAGTAGG GGACTTGCTG CAATTTTCTG AAACGAAGCT ACAAGAACAT 
TACGGAGTAA ATACTGGTAC TTGGTTATGG AATATAGCAA GAGGGATCAG TGGAGAAGAA 
GTGCAAGGTC GCCTTCTCCC CAAAAGTCAT GGTTCTGGAA AGACATTTCC CGGACCTCGT 
GCTTTGAAGT CACTCTCAAC TGTTCAGCAT TGGCTGAATC AGCTTTCCGA GGAACTAAGC 
GAGCGCCTCG GTTCTGACTT GGAGCAGAAT AAGCGGATTG CAAGCACCCT TACCCTTCAT 
GCTAGCGCTT TCAGATCAAA GGATTCAGAT TCTCATAAGA AATTTCCTTC AAAATCATGT 
CCTATGAGAT ATGGAGTAAC CAAGATCCAA GAAGATGCCT TTAACTTGTT CCAAGCTGCC 
TTACGTGAGT ACATGGGATC CTTCGGGATC AAACCTCAAG GAAATAAGCT AGAGACATGG 
AGGATAACAG GTCTTTCTGT TTCAGCGAGT AAAATTGTTG ATATACCATC TGGAACAAGC 
TCGATCATGA GATATTTTCA AAGTCAACCT ACTGTTCCTT CTCGTTCAGC AGATGGTTGT 
GTTCAAGGAA ATGTAGCGAT GACTGCTTCA GCTAGCGAAG GATGTTCAGA ACAGAGGTCA 
ACAGAAACTC AAGCAGCAAT GCCTGAAGTA GACACCGGTG TAACTTATAC TTTGCCTAAT 
TTTGAAAACC AAGATAAAGA CATTGATCTA GTCTCCGAAA AGGATGTTGT CTCTTGTCCA 
TCAAACGAAG CAACGGATGT TTCTACCCAA TCAGAATCAA ACAAAGGCAC ACAGACAAAG 
AAAATTGGGC GGAAGATGAA CAACTCGAAA GAAAAGAATC GGGGAATGCC TTCAATTGTC 
GATATCTTTA AGAATTACAA TGCAACACCT CCATCAAAAC AAGAGACTCA AGAAGATTCC 
ACAGTGTCAT CAGCCAGTAA AAGAGCAAAG TTAAGTAGCA GCAGCCATAA TAGTCAAGTG 
AATCAAGAGG TAGAAGAGAG TAGAGAAACA GATTGGGGAT ATAAGACTGA TGAAATCGAT 
CAGTCAGTTT TCGATGAATT ACCGGTTGAG ATTCAACGAG AACTCAGGAG TTTCCTACGC 
ACCAATAAGC AGTTTAACAC CGGTAAAAGC AAAGGTGATG GTTCTACTTC AAGCATTGCT 
CATTATTTTC CACCATTGAA TAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLH ( NM_123841.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_568638.2 Arabidopsis thaliana Y-family DNA polymerase H (POLH), mRNA.

Price & Availability↑

CloneID	OAb20556
Clone ID Related Accession (Same CDS sequence)	NM_123841.5
Accession Version	NM_123841.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Y-family DNA polymerase H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_123841.4.

ATGCCGGTAG CGAGACCGGA AGCATCGGAT GCTAGAGTTA TCGCTCATGT CGACATGGAT 
TGCTTCTACG TTCAAGTGGA GCAGAGGAAG CAACCGGAGT TGCGAGGACT GCCTAGTGCA 
GTTGTACAGT ACAATGAATG GCAAGGTGGT GGTTTGATTG CTGTTAGTTA CGAAGCACGT 
AAATGTGGCG TAAAGCGGTC GATGAGAGGT GATGAGGCTA AAGCTGCTTG TCCTCAGATT 
CAATTGGTTC AAGTTCCTGT GGCTCGTGGT AAAGCTGACC TTAATTTATA TCGCAGTGCC 
GGTTCTGAGG TGGTTTCGAT TCTAGCAAAA AGCGGAAAAT GTGAGAGGGC TTCGATTGAT 
GAAGTGTATC TTGACCTCAC TGATGCAGCA GAAAGCATGC TTGCTGATGC ACCTCCAGAG 
AGTTTGGAAT TGATAGATGA GGAAGTTCTT AAATCCCATA TTCTTGGAAT GAACAGAGAG 
GATGGAGATG ATTTCAAGGA GAGTGTCCGC AATTGGATTT GTCGAGAAGA TGCTGATCGA 
CGTGATAAGT TATTAAGTTG TGGAATTATC ATTGTTGCAG AACTCCGGAA ACAGGTGCTT 
AAGGAGACTG AGTTTACTTG TTCTGCTGGC ATAGCCCATA ACAAGATGCT AGCCAAACTT 
GCTAGTGGAA TGAACAAACC TGCGCAGCAG ACAGTTGTAC CATATGCAGC TGTACAGGAA 
TTACTAAGTT CCTTGCCAAT AAAGAAAATG AAACAACTGG GAGGAAAGCT GGGCACTAGC 
TTGCAAACTG ACTTGGGAGT TGACACAGTA GGGGACTTGC TGCAATTTTC TGAAACGAAG 
CTACAAGAAC ATTACGGAGT AAATACTGGT ACTTGGTTAT GGAATATAGC AAGAGGGATC 
AGTGGAGAAG AAGTGCAAGG TCGCCTTCTC CCCAAAAGTC ATGGTTCTGG AAAGACATTT 
CCCGGACCTC GTGCTTTGAA GTCACTCTCA ACTGTTCAGC ATTGGCTGAA TCAGCTTTCC 
GAGGAACTAA GCGAGCGCCT CGGTTCTGAC TTGGAGCAGA ATAAGCGGAT TGCAAGCACC 
CTTACCCTTC ATGCTAGCGC TTTCAGATCA AAGGATTCAG ATTCTCATAA GAAATTTCCT 
TCAAAATCAT GTCCTATGAG ATATGGAGTA ACCAAGATCC AAGAAGATGC CTTTAACTTG 
TTCCAAGCTG CCTTACGTGA GTACATGGGA TCCTTCGGGA TCAAACCTCA AGGAAATAAG 
CTAGAGACAT GGAGGATAAC AGGTCTTTCT GTTTCAGCGA GTAAAATTGT TGATATACCA 
TCTGGAACAA GCTCGATCAT GAGATATTTT CAAAGTCAAC CTACTGTTCC TTCTCGTTCA 
GCAGATGGTT GTGTTCAAGG AAATGTAGCG ATGACTGCTT CAGCTAGCGA AGGATGTTCA 
GAACAGAGGT CAACAGAAAC TCAAGCAGCA ATGCCTGAAG TAGACACCGG TGTAACTTAT 
ACTTTGCCTA ATTTTGAAAA CCAAGATAAA GACATTGATC TAGTCTCCGA AAAGGATGTT 
GTCTCTTGTC CATCAAACGA AGCAACGGAT GTTTCTACCC AATCAGAATC AAACAAAGGC 
ACACAGACAA AGAAAATTGG GCGGAAGATG AACAACTCGA AAGAAAAGAA TCGGGGAATG 
CCTTCAATTG TCGATATCTT TAAGAATTAC AATGCAACAC CTCCATCAAA ACAAGAGACT 
CAAGAAGATT CCACAGTGTC ATCAGCCAGT AAAAGAGCAA AGTTAAGTAG CAGCAGCCAT 
AATAGTCAAG TGAATCAAGA GGTAGAAGAG AGTAGAGAAA CAGATTGGGG ATATAAGACT 
GATGAAATCG ATCAGTCAGT TTTCGATGAA TTACCGGTTG AGATTCAACG AGAACTCAGG 
AGTTTCCTAC GCACCAATAA GCAGTTTAAC ACCGGTAAAA GCAAAGGTGA TGGTTCTACT 
TCAAGCATTG CTCATTATTT TCCACCATTG AATAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_568638.2
CDS	118..2136
Translation	MPVARPEASDARVIAHVDMDCFYVQVEQRKQPELRGLPSAVVQYNEWQGGGLIAVSYEARKCGVKRSMRGDEAKAACPQIQLVQVPVARGKADLNLYRSAGSEVVSILAKSGKCERASIDEVYLDLTDAAESMLADAPPESLELIDEEVLKSHILGMNREDGDDFKESVRNWICREDADRRDKLLSCGIIIVAELRKQVLKETEFTCSAGIAHNKMLAKLASGMNKPAQQTVVPYAAVQELLSSLPIKKMKQLGGKLGTSLQTDLGVDTVGDLLQFSETKLQEHYGVNTGTWLWNIARGISGEEVQGRLLPKSHGSGKTFPGPRALKSLSTVQHWLNQLSEELSERLGSDLEQNKRIASTLTLHASAFRSKDSDSHKKFPSKSCPMRYGVTKIQEDAFNLFQAALREYMGSFGIKPQGNKLETWRITGLSVSASKIVDIPSGTSSIMRYFQSQPTVPSRSADGCVQGNVAMTASASEGCSEQRSTETQAAMPEVDTGVTYTLPNFENQDKDIDLVSEKDVVSCPSNEATDVSTQSESNKGTQTKKIGRKMNNSKEKNRGMPSIVDIFKNYNATPPSKQETQEDSTVSSASKRAKLSSSSHNSQVNQEVEESRETDWGYKTDEIDQSVFDELPVEIQRELRSFLRTNKQFNTGKSKGDGSTSSIAHYFPPLNR

Target ORF information:

RefSeq Version	NM_123841.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Y-family DNA polymerase H (POLH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_123841.5

ORF Insert Sequence:

ATGCCGGTAG CGAGACCGGA AGCATCGGAT GCTAGAGTTA TCGCTCATGT CGACATGGAT 
TGCTTCTACG TTCAAGTGGA GCAGAGGAAG CAACCGGAGT TGCGAGGACT GCCTAGTGCA 
GTTGTACAGT ACAATGAATG GCAAGGTGGT GGTTTGATTG CTGTTAGTTA CGAAGCACGT 
AAATGTGGCG TAAAGCGGTC GATGAGAGGT GATGAGGCTA AAGCTGCTTG TCCTCAGATT 
CAATTGGTTC AAGTTCCTGT GGCTCGTGGT AAAGCTGACC TTAATTTATA TCGCAGTGCC 
GGTTCTGAGG TGGTTTCGAT TCTAGCAAAA AGCGGAAAAT GTGAGAGGGC TTCGATTGAT 
GAAGTGTATC TTGACCTCAC TGATGCAGCA GAAAGCATGC TTGCTGATGC ACCTCCAGAG 
AGTTTGGAAT TGATAGATGA GGAAGTTCTT AAATCCCATA TTCTTGGAAT GAACAGAGAG 
GATGGAGATG ATTTCAAGGA GAGTGTCCGC AATTGGATTT GTCGAGAAGA TGCTGATCGA 
CGTGATAAGT TATTAAGTTG TGGAATTATC ATTGTTGCAG AACTCCGGAA ACAGGTGCTT 
AAGGAGACTG AGTTTACTTG TTCTGCTGGC ATAGCCCATA ACAAGATGCT AGCCAAACTT 
GCTAGTGGAA TGAACAAACC TGCGCAGCAG ACAGTTGTAC CATATGCAGC TGTACAGGAA 
TTACTAAGTT CCTTGCCAAT AAAGAAAATG AAACAACTGG GAGGAAAGCT GGGCACTAGC 
TTGCAAACTG ACTTGGGAGT TGACACAGTA GGGGACTTGC TGCAATTTTC TGAAACGAAG 
CTACAAGAAC ATTACGGAGT AAATACTGGT ACTTGGTTAT GGAATATAGC AAGAGGGATC 
AGTGGAGAAG AAGTGCAAGG TCGCCTTCTC CCCAAAAGTC ATGGTTCTGG AAAGACATTT 
CCCGGACCTC GTGCTTTGAA GTCACTCTCA ACTGTTCAGC ATTGGCTGAA TCAGCTTTCC 
GAGGAACTAA GCGAGCGCCT CGGTTCTGAC TTGGAGCAGA ATAAGCGGAT TGCAAGCACC 
CTTACCCTTC ATGCTAGCGC TTTCAGATCA AAGGATTCAG ATTCTCATAA GAAATTTCCT 
TCAAAATCAT GTCCTATGAG ATATGGAGTA ACCAAGATCC AAGAAGATGC CTTTAACTTG 
TTCCAAGCTG CCTTACGTGA GTACATGGGA TCCTTCGGGA TCAAACCTCA AGGAAATAAG 
CTAGAGACAT GGAGGATAAC AGGTCTTTCT GTTTCAGCGA GTAAAATTGT TGATATACCA 
TCTGGAACAA GCTCGATCAT GAGATATTTT CAAAGTCAAC CTACTGTTCC TTCTCGTTCA 
GCAGATGGTT GTGTTCAAGG AAATGTAGCG ATGACTGCTT CAGCTAGCGA AGGATGTTCA 
GAACAGAGGT CAACAGAAAC TCAAGCAGCA ATGCCTGAAG TAGACACCGG TGTAACTTAT 
ACTTTGCCTA ATTTTGAAAA CCAAGATAAA GACATTGATC TAGTCTCCGA AAAGGATGTT 
GTCTCTTGTC CATCAAACGA AGCAACGGAT GTTTCTACCC AATCAGAATC AAACAAAGGC 
ACACAGACAA AGAAAATTGG GCGGAAGATG AACAACTCGA AAGAAAAGAA TCGGGGAATG 
CCTTCAATTG TCGATATCTT TAAGAATTAC AATGCAACAC CTCCATCAAA ACAAGAGACT 
CAAGAAGATT CCACAGTGTC ATCAGCCAGT AAAAGAGCAA AGTTAAGTAG CAGCAGCCAT 
AATAGTCAAG TGAATCAAGA GGTAGAAGAG AGTAGAGAAA CAGATTGGGG ATATAAGACT 
GATGAAATCG ATCAGTCAGT TTTCGATGAA TTACCGGTTG AGATTCAACG AGAACTCAGG 
AGTTTCCTAC GCACCAATAA GCAGTTTAAC ACCGGTAAAA GCAAAGGTGA TGGTTCTACT 
TCAAGCATTG CTCATTATTT TCCACCATTG AATAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLH ( NM_001344572.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001332153.1 Arabidopsis thaliana Y-family DNA polymerase H (POLH), mRNA.

Price & Availability↑

CloneID	OAb41595
Clone ID Related Accession (Same CDS sequence)	NM_001344572.1
Accession Version	NM_001344572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Y-family DNA polymerase H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGAGAGGTG ATGAGGCTAA AGCTGCTTGT CCTCAGATTC AATTGGTTCA AGTTCCTGTG 
GCTCGTGGTA AAGCTGACCT TAATTTATAT CGCAGTGCCG GTTCTGAGGT GGTTTCGATT 
CTAGCAAAAA GCGGAAAATG TGAGAGGGCT TCGATTGATG AAGTGTATCT TGACCTCACT 
GATGCAGCAG AAAGCATGCT TGCTGATGCA CCTCCAGAGA GTTTGGAATT GATAGATGAG 
GAAGTTCTTA AATCCCATAT TCTTGGAATG AACAGAGAGG ATGGAGATGA TTTCAAGGAG 
AGTGTCCGCA ATTGGATTTG TCGAGAAGAT GCTGATCGAC GTGATAAGTT ATTAAGTTGT 
GGAATTATCA TTGTTGCAGA ACTCCGGAAA CAGGTGCTTA AGGAGACTGA GTTTACTTGT 
TCTGCTGGCA TAGCCCATAA CAAGATGCTA GCCAAACTTG CTAGTGGAAT GAACAAACCT 
GCGCAGCAGA CAGTTGTACC ATATGCAGCT GTACAGGAAT TACTAAGTTC CTTGCCAATA 
AAGAAAATGA AACAACTGGG AGGAAAGCTG GGCACTAGCT TGCAAACTGA CTTGGGAGTT 
GACACAGTAG GGGACTTGCT GCAATTTTCT GAAACGAAGC TACAAGAACA TTACGGAGTA 
AATACTGGTA CTTGGTTATG GAATATAGCA AGAGGGATCA GTGGAGAAGA AGTGCAAGGT 
CGCCTTCTCC CCAAAAGTCA TGGTTCTGGA AAGACATTTC CCGGACCTCG TGCTTTGAAG 
TCACTCTCAA CTGTTCAGCA TTGGCTGAAT CAGCTTTCCG AGGAACTAAG CGAGCGCCTC 
GGTTCTGACT TGGAGCAGAA TAAGCGGATT GCAAGCACCC TTACCCTTCA TGCTAGCGCT 
TTCAGATCAA AGGATTCAGA TTCTCATAAG AAATTTCCTT CAAAATCATG TCCTATGAGA 
TATGGAGTAA CCAAGATCCA AGAAGATGCC TTTAACTTGT TCCAAGCTGC CTTACGTGAG 
TACATGGGAT CCTTCGGGAT CAAACCTCAA GGAAATAAGC TAGAGACATG GAGGATAACA 
GGTCTTTCTG TTTCAGCGAG TAAAATTGTT GATATACCAT CTGGAACAAG CTCGATCATG 
AGATATTTTC AAAGTCAACC TACTGTTCCT TCTCGTTCAG CAGATGGTTG TGTTCAAGGA 
AATGTAGCGA TGACTGCTTC AGCTAGCGAA GGATGTTCAG AACAGAGGTC AACAGAAACT 
CAAGCAGCAA TGCCTGAAGT AGACACCGGT GTAACTTATA CTTTGCCTAA TTTTGAAAAC 
CAAGATAAAG ACATTGATCT AGTCTCCGAA AAGGATGTTG TCTCTTGTCC ATCAAACGAA 
GCAACGGATG TTTCTACCCA ATCAGAATCA AACAAAGGCA CACAGACAAA GAAAATTGGG 
CGGAAGATGA ACAACTCGAA AGAAAAGAAT CGGGGAATGC CTTCAATTGT CGATATCTTT 
AAGAATTACA ATGCAACACC TCCATCAAAA CAAGAGACTC AAGAAGATTC CACAGTGTCA 
TCAGCCAGTA AAAGAGCAAA GTTAAGTAGC AGCAGCCATA ATAGTCAAGT GAATCAAGAG 
GTAGAAGAGA GTAGAGAAAC AGATTGGGGA TATAAGACTG ATGAAATCGA TCAGTCAGTT 
TTCGATGAAT TACCGGTTGA GATTCAACGA GAACTCAGGA GTTTCCTACG CACCAATAAG 
CAGTTTAACA CCGGTAAAAG CAAAGGTGAT GGTTCTACTT CAAGCATTGC TCATTATTTT 
CCACCATTGA ATAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001332153.1
CDS	583..2400
Translation	MRGDEAKAACPQIQLVQVPVARGKADLNLYRSAGSEVVSILAKSGKCERASIDEVYLDLTDAAESMLADAPPESLELIDEEVLKSHILGMNREDGDDFKESVRNWICREDADRRDKLLSCGIIIVAELRKQVLKETEFTCSAGIAHNKMLAKLASGMNKPAQQTVVPYAAVQELLSSLPIKKMKQLGGKLGTSLQTDLGVDTVGDLLQFSETKLQEHYGVNTGTWLWNIARGISGEEVQGRLLPKSHGSGKTFPGPRALKSLSTVQHWLNQLSEELSERLGSDLEQNKRIASTLTLHASAFRSKDSDSHKKFPSKSCPMRYGVTKIQEDAFNLFQAALREYMGSFGIKPQGNKLETWRITGLSVSASKIVDIPSGTSSIMRYFQSQPTVPSRSADGCVQGNVAMTASASEGCSEQRSTETQAAMPEVDTGVTYTLPNFENQDKDIDLVSEKDVVSCPSNEATDVSTQSESNKGTQTKKIGRKMNNSKEKNRGMPSIVDIFKNYNATPPSKQETQEDSTVSSASKRAKLSSSSHNSQVNQEVEESRETDWGYKTDEIDQSVFDELPVEIQRELRSFLRTNKQFNTGKSKGDGSTSSIAHYFPPLNR

Target ORF information:

RefSeq Version	NM_001344572.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Y-family DNA polymerase H (POLH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001344572.1

ORF Insert Sequence:

ATGAGAGGTG ATGAGGCTAA AGCTGCTTGT CCTCAGATTC AATTGGTTCA AGTTCCTGTG 
GCTCGTGGTA AAGCTGACCT TAATTTATAT CGCAGTGCCG GTTCTGAGGT GGTTTCGATT 
CTAGCAAAAA GCGGAAAATG TGAGAGGGCT TCGATTGATG AAGTGTATCT TGACCTCACT 
GATGCAGCAG AAAGCATGCT TGCTGATGCA CCTCCAGAGA GTTTGGAATT GATAGATGAG 
GAAGTTCTTA AATCCCATAT TCTTGGAATG AACAGAGAGG ATGGAGATGA TTTCAAGGAG 
AGTGTCCGCA ATTGGATTTG TCGAGAAGAT GCTGATCGAC GTGATAAGTT ATTAAGTTGT 
GGAATTATCA TTGTTGCAGA ACTCCGGAAA CAGGTGCTTA AGGAGACTGA GTTTACTTGT 
TCTGCTGGCA TAGCCCATAA CAAGATGCTA GCCAAACTTG CTAGTGGAAT GAACAAACCT 
GCGCAGCAGA CAGTTGTACC ATATGCAGCT GTACAGGAAT TACTAAGTTC CTTGCCAATA 
AAGAAAATGA AACAACTGGG AGGAAAGCTG GGCACTAGCT TGCAAACTGA CTTGGGAGTT 
GACACAGTAG GGGACTTGCT GCAATTTTCT GAAACGAAGC TACAAGAACA TTACGGAGTA 
AATACTGGTA CTTGGTTATG GAATATAGCA AGAGGGATCA GTGGAGAAGA AGTGCAAGGT 
CGCCTTCTCC CCAAAAGTCA TGGTTCTGGA AAGACATTTC CCGGACCTCG TGCTTTGAAG 
TCACTCTCAA CTGTTCAGCA TTGGCTGAAT CAGCTTTCCG AGGAACTAAG CGAGCGCCTC 
GGTTCTGACT TGGAGCAGAA TAAGCGGATT GCAAGCACCC TTACCCTTCA TGCTAGCGCT 
TTCAGATCAA AGGATTCAGA TTCTCATAAG AAATTTCCTT CAAAATCATG TCCTATGAGA 
TATGGAGTAA CCAAGATCCA AGAAGATGCC TTTAACTTGT TCCAAGCTGC CTTACGTGAG 
TACATGGGAT CCTTCGGGAT CAAACCTCAA GGAAATAAGC TAGAGACATG GAGGATAACA 
GGTCTTTCTG TTTCAGCGAG TAAAATTGTT GATATACCAT CTGGAACAAG CTCGATCATG 
AGATATTTTC AAAGTCAACC TACTGTTCCT TCTCGTTCAG CAGATGGTTG TGTTCAAGGA 
AATGTAGCGA TGACTGCTTC AGCTAGCGAA GGATGTTCAG AACAGAGGTC AACAGAAACT 
CAAGCAGCAA TGCCTGAAGT AGACACCGGT GTAACTTATA CTTTGCCTAA TTTTGAAAAC 
CAAGATAAAG ACATTGATCT AGTCTCCGAA AAGGATGTTG TCTCTTGTCC ATCAAACGAA 
GCAACGGATG TTTCTACCCA ATCAGAATCA AACAAAGGCA CACAGACAAA GAAAATTGGG 
CGGAAGATGA ACAACTCGAA AGAAAAGAAT CGGGGAATGC CTTCAATTGT CGATATCTTT 
AAGAATTACA ATGCAACACC TCCATCAAAA CAAGAGACTC AAGAAGATTC CACAGTGTCA 
TCAGCCAGTA AAAGAGCAAA GTTAAGTAGC AGCAGCCATA ATAGTCAAGT GAATCAAGAG 
GTAGAAGAGA GTAGAGAAAC AGATTGGGGA TATAAGACTG ATGAAATCGA TCAGTCAGTT 
TTCGATGAAT TACCGGTTGA GATTCAACGA GAACTCAGGA GTTTCCTACG CACCAATAAG 
CAGTTTAACA CCGGTAAAAG CAAAGGTGAT GGTTCTACTT CAAGCATTGC TCATTATTTT 
CCACCATTGA ATAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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POLH cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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