KEA3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	KEA3
Entrez Gene ID	825822
Full Name	K+ efflux antiporter 3
Synonyms	ATKEA3,K+ efflux antiporter 3,T4B21.3,T4B21_3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_116723.5	NP_567272.4	K+ efflux antiporter 3
NM_001203746.1	NP_001190675.1	K+ efflux antiporter 3

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os12g0617800	NP_001067289.1
Arabidopsis thaliana (thale cress)	KEA3	NP_001190675.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings suggest that fine regulation of K+ efflux antiporter KEA3 activity is necessary for optimizing photosynthesis.
Title: Fine-tuned regulation of the K

KEA3 activity is regulated by a mechanism involving its lumen-localized C-terminus, which lowers KEA3 activity in high light.
Title: Regulation and Levels of the Thylakoid K+/H+ Antiporter KEA3 Shape the Dynamic Response of Photosynthesis in Fluctuating Light.

KEA3 is critical for high photosynthetic efficiency under fluctuating light.
Title: Ion antiport accelerates photosynthetic acclimation in fluctuating light environments.

role of inner envelope KEA1 and KEA2 and thylakoid KEA3 transporters in chloroplast osmoregulation, integrity, and ion and pH homeostasis
Title: Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis.

The following KEA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KEA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb14313	NM_116723.5 Latest version!	Arabidopsis thaliana K+ efflux antiporter 3 (KEA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb29955	NM_001203746.1 Latest version!	Arabidopsis thaliana K+ efflux antiporter 3 (KEA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KEA3 ( NM_116723.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567272.4 Arabidopsis thaliana K+ efflux antiporter 3 (KEA3), mRNA.

Price & Availability↑

CloneID	OAb14313
Clone ID Related Accession (Same CDS sequence)	NM_116723.5
Accession Version	NM_116723.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	K+ efflux antiporter 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Jun 19, 2009 this sequence version replaced NM_116723.4.

ATGGCAATTA GTACTATGTT AGGGTCCATC TCTTGTTGCC CCTCTCCCAA GGGGTATGAG 
ATGGTGAAAC AACATAGTGT TCGATTAAAG CATTGCGTTT TCACGGTGAA ATCGAGTGTG 
CCTGTGTATT CGGAAGGCGT AAACGATGGG ATTAAGTTGC ATTCTTTTGG GAATTTAGTT 
AAAAAGAAGG TTTTTCTGGA TACCTCTAAG AGATTCTATT TCCAAGGGAG ATGGTCTGAA 
TCGTCAGGGA GGAGAGTGGA GACTTATGCT GGAGTAGATG TAGCGAGTGC TGTTGATGTA 
ATCAACGATC TTGGATTCGA TACTTTGACC TTCTTGATGG TTACTGTTAT TATTGTCCCT 
GCATTCAGAA TTCTCAAAGC TAGTCCGATA CTTGGTTTCT TCTTTGCTGG TGTTGTACTC 
AACCAATTTG GTTTGATTAG AAATCTCACA GATGTTAAAG TTCTTTCAGA ATGGGGGATT 
CTTTTCCTGC TCTTTGAGAT GGGTCTCGAG CTTTCACTTG CTCGTCTGAA AGCTCTTGCA 
AAATTTGCTT TTGGCATGGG ACTAACTCAG GTTTTGTTAT GCACGGCTGC ATTCACTGCT 
TTTGAACTCC CACCAAATGG AGCCATCGGA ACAAAAATTC TGGAATTCCT CTTTCATTCA 
AGGCCTGACT TGGTCAACAT TAGAAGCATT GATGAGGCCG TTGTCATTGG CGCTGCTCTA 
TCATTGTCAT CTTCAGCTTT TGTTCTACAG CTTCTTGCAG AGAAAGGTGA GCTCCCTACA 
AGATTTGGCT CAGCAACCTT GGGAATTCTT CTTCTACAGG ATATAGCTGT TGTGCCGTTA 
CTAGTCATTC TTCCAGTGTT GGAAAGCCAG GATATTGGTG GGGAAAGCAT ATGGCCAATG 
CTTGCAAAAG AAAGTGCAAA GGCTCTTGGT GGGTTGGGCA TCCTCTCTCT TGGAGGAAAG 
TTCTTTCTCC GTAGAATTTT CGAGGTTGTT GCAGAAACAA GAAGCTCAGA AGCTTTTGTG 
GCCCTTTGCC TTCTCACAGT TGCTGGGACT TCACTTGTGA CTCAATGGCT TGGTTTCAGC 
GATACGCTTG GAGCTTTTCT TGCTGGAGCA CTTCTAGCAG AAACAAATTT CCGAACACAA 
ATTGAAGCTG ATATTAGGCC ATTTAGAGGT TTACTTCTCG GTTTGTTCTT TGTAACCACA 
GGAACTTCCA TTGACATGGA GGTTCTGTTT CGAGAATGGC CTAATGTCCT CTCACTCTTG 
GGTGGTCTGA TTGTGATCAA AACACTGATT ATAACCGCAA TCGGTCCCCG AGTTGGCCTC 
ACAATACAAG AAAGCGTAAG AGTTGGCTTT CTTCTTTCCC AGGGAGGAGA ATTTGCATTT 
GTTGTGTTCT CTCTAGCCAA CAGGCTAGGA GTGCTTCCAA ATGAGCTGAA CAAACTGCTC 
ATTATCGTAG TTGTTTTGTC TATGGCATTA ACACCTTATC TTAACCAACT TGGAAGAAAA 
GCCGCCGATT TTCTTGATGA GAGACTTGAT CCCGGAGAGA AAATAGGTGA AGATGTGAAC 
TTCGATGTTA GTGAATCAAT TGTCATCATT GGATTTGGAC AAATGGGTCA GGTTCTGGCC 
AATTTTTTGT CAACGCCATT GGTCTCAGAT AGTGATCTTG TGGGATGGCC TTATATTGGT 
TTTGATCTGA ATCCTGCTGT AGTGAAGGAA TCAAGGAAAC TCGGTTTCCC GATATTGTAT 
GGAGACGGAT CTCGACCATC GGTTTTGCAA TCTGCAGGGG TTTCATCTCC TAAAGCTATC 
ATGATAATGT ATAAAGGCAA GAAGAGAACC ACTGAGGCTG TTCAAAGACT CCGTCTTGCC 
TTCCCCGGGG TTATTTCTCT CTTCCTTCTT CCTTTTGTCA ATAAAATCGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567272.4
CDS	292..2205
Translation	MAISTMLGSISCCPSPKGYEMVKQHSVRLKHCVFTVKSSVPVYSEGVNDGIKLHSFGNLVKKKVFLDTSKRFYFQGRWSESSGRRVETYAGVDVASAVDVINDLGFDTLTFLMVTVIIVPAFRILKASPILGFFFAGVVLNQFGLIRNLTDVKVLSEWGILFLLFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHSRPDLVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVILPVLESQDIGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAFVALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFAFVVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDVNFDVSESIVIIGFGQMGQVLANFLSTPLVSDSDLVGWPYIGFDLNPAVVKESRKLGFPILYGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGVISLFLLPFVNKIV

Target ORF information:

RefSeq Version	NM_116723.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana K+ efflux antiporter 3 (KEA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_116723.5

ORF Insert Sequence:

ATGGCAATTA GTACTATGTT AGGGTCCATC TCTTGTTGCC CCTCTCCCAA GGGGTATGAG 
ATGGTGAAAC AACATAGTGT TCGATTAAAG CATTGCGTTT TCACGGTGAA ATCGAGTGTG 
CCTGTGTATT CGGAAGGCGT AAACGATGGG ATTAAGTTGC ATTCTTTTGG GAATTTAGTT 
AAAAAGAAGG TTTTTCTGGA TACCTCTAAG AGATTCTATT TCCAAGGGAG ATGGTCTGAA 
TCGTCAGGGA GGAGAGTGGA GACTTATGCT GGAGTAGATG TAGCGAGTGC TGTTGATGTA 
ATCAACGATC TTGGATTCGA TACTTTGACC TTCTTGATGG TTACTGTTAT TATTGTCCCT 
GCATTCAGAA TTCTCAAAGC TAGTCCGATA CTTGGTTTCT TCTTTGCTGG TGTTGTACTC 
AACCAATTTG GTTTGATTAG AAATCTCACA GATGTTAAAG TTCTTTCAGA ATGGGGGATT 
CTTTTCCTGC TCTTTGAGAT GGGTCTCGAG CTTTCACTTG CTCGTCTGAA AGCTCTTGCA 
AAATTTGCTT TTGGCATGGG ACTAACTCAG GTTTTGTTAT GCACGGCTGC ATTCACTGCT 
TTTGAACTCC CACCAAATGG AGCCATCGGA ACAAAAATTC TGGAATTCCT CTTTCATTCA 
AGGCCTGACT TGGTCAACAT TAGAAGCATT GATGAGGCCG TTGTCATTGG CGCTGCTCTA 
TCATTGTCAT CTTCAGCTTT TGTTCTACAG CTTCTTGCAG AGAAAGGTGA GCTCCCTACA 
AGATTTGGCT CAGCAACCTT GGGAATTCTT CTTCTACAGG ATATAGCTGT TGTGCCGTTA 
CTAGTCATTC TTCCAGTGTT GGAAAGCCAG GATATTGGTG GGGAAAGCAT ATGGCCAATG 
CTTGCAAAAG AAAGTGCAAA GGCTCTTGGT GGGTTGGGCA TCCTCTCTCT TGGAGGAAAG 
TTCTTTCTCC GTAGAATTTT CGAGGTTGTT GCAGAAACAA GAAGCTCAGA AGCTTTTGTG 
GCCCTTTGCC TTCTCACAGT TGCTGGGACT TCACTTGTGA CTCAATGGCT TGGTTTCAGC 
GATACGCTTG GAGCTTTTCT TGCTGGAGCA CTTCTAGCAG AAACAAATTT CCGAACACAA 
ATTGAAGCTG ATATTAGGCC ATTTAGAGGT TTACTTCTCG GTTTGTTCTT TGTAACCACA 
GGAACTTCCA TTGACATGGA GGTTCTGTTT CGAGAATGGC CTAATGTCCT CTCACTCTTG 
GGTGGTCTGA TTGTGATCAA AACACTGATT ATAACCGCAA TCGGTCCCCG AGTTGGCCTC 
ACAATACAAG AAAGCGTAAG AGTTGGCTTT CTTCTTTCCC AGGGAGGAGA ATTTGCATTT 
GTTGTGTTCT CTCTAGCCAA CAGGCTAGGA GTGCTTCCAA ATGAGCTGAA CAAACTGCTC 
ATTATCGTAG TTGTTTTGTC TATGGCATTA ACACCTTATC TTAACCAACT TGGAAGAAAA 
GCCGCCGATT TTCTTGATGA GAGACTTGAT CCCGGAGAGA AAATAGGTGA AGATGTGAAC 
TTCGATGTTA GTGAATCAAT TGTCATCATT GGATTTGGAC AAATGGGTCA GGTTCTGGCC 
AATTTTTTGT CAACGCCATT GGTCTCAGAT AGTGATCTTG TGGGATGGCC TTATATTGGT 
TTTGATCTGA ATCCTGCTGT AGTGAAGGAA TCAAGGAAAC TCGGTTTCCC GATATTGTAT 
GGAGACGGAT CTCGACCATC GGTTTTGCAA TCTGCAGGGG TTTCATCTCC TAAAGCTATC 
ATGATAATGT ATAAAGGCAA GAAGAGAACC ACTGAGGCTG TTCAAAGACT CCGTCTTGCC 
TTCCCCGGGG TTATTTCTCT CTTCCTTCTT CCTTTTGTCA ATAAAATCGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KEA3 ( NM_001203746.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190675.1 Arabidopsis thaliana K+ efflux antiporter 3 (KEA3), mRNA.

Price & Availability↑

CloneID	OAb29955
Clone ID Related Accession (Same CDS sequence)	NM_001203746.1
Accession Version	NM_001203746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	K+ efflux antiporter 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGCAATTA GTACTATGTT AGGGTCCATC TCTTGTTGCC CCTCTCCCAA GGGGTATGAG 
ATGGTGAAAC AACATAGTGT TCGATTAAAG CATTGCGTTT TCACGGTGAA ATCGAGTGTG 
CCTGTGTATT CGGAAGGCGT AAACGATGGG ATTAAGTTGC ATTCTTTTGG GAATTTAGTT 
AAAAAGAAGG TTTTTCTGGA TACCTCTAAG AGATTCTATT TCCAAGGGAG ATGGTCTGAA 
TCGTCAGGGA GGAGAGTGGA GACTTATGCT GGAGTAGATG TAGCGAGTGC TGTTGATGTA 
ATCAACGATC TTGGATTCGA TACTTTGACC TTCTTGATGG TTACTGTTAT TATTGTCCCT 
GCATTCAGAA TTCTCAAAGC TAGTCCGATA CTTGGTTTCT TCTTTGCTGG TGTTGTACTC 
AACCAATTTG GTTTGATTAG AAATCTCACA GATGTTAAAG TTCTTTCAGA ATGGGGGATT 
CTTTTCCTGC TCTTTGAGAT GGGTCTCGAG CTTTCACTTG CTCGTCTGAA AGCTCTTGCA 
AAATTTGCTT TTGGCATGGG ACTAACTCAG GTTTTGTTAT GCACGGCTGC ATTCACTGCT 
TTTGAACTCC CACCAAATGG AGCCATCGGA ACAAAAATTC TGGAATTCCT CTTTCATTCA 
AGGCCTGACT TGGTCAACAT TAGAAGCATT GATGAGGCCG TTGTCATTGG CGCTGCTCTA 
TCATTGTCAT CTTCAGCTTT TGTTCTACAG CTTCTTGCAG AGAAAGGTGA GCTCCCTACA 
AGATTTGGCT CAGCAACCTT GGGAATTCTT CTTCTACAGG ATATAGCTGT TGTGCCGTTA 
CTAGTCATTC TTCCAGTGTT GGAAAGCCAG GATATTGGTG GGGAAAGCAT ATGGCCAATG 
CTTGCAAAAG AAAGTGCAAA GGCTCTTGGT GGGTTGGGCA TCCTCTCTCT TGGAGGAAAG 
TTCTTTCTCC GTAGAATTTT CGAGGTTGTT GCAGAAACAA GAAGCTCAGA AGCTTTTGTG 
GCCCTTTGCC TTCTCACAGT TGCTGGGACT TCACTTGTGA CTCAATGGCT TGGTTTCAGC 
GATACGCTTG GAGCTTTTCT TGCTGGAGCA CTTCTAGCAG AAACAAATTT CCGAACACAA 
ATTGAAGCTG ATATTAGGCC ATTTAGAGGT TTACTTCTCG GTTTGTTCTT TGTAACCACA 
GGAACTTCCA TTGACATGGA GGTTCTGTTT CGAGAATGGC CTAATGTCCT CTCACTCTTG 
GGTGGTCTGA TTGTGATCAA AACACTGATT ATAACCGCAA TCGGTCCCCG AGTTGGCCTC 
ACAATACAAG AAAGCGTAAG AGTTGGCTTT CTTCTTTCCC AGGGAGGAGA ATTTGCATTT 
GTTGTGTTCT CTCTAGCCAA CAGGCTAGGA GTGCTTCCAA ATGAGCTGAA CAAACTGCTC 
ATTATCGTAG TTGTTTTGTC TATGGCATTA ACACCTTATC TTAACCAACT TGGAAGAAAA 
GCCGCCGATT TTCTTGATGA GAGACTTGAT CCCGGAGAGA AAATAGGTGA AGATGTGAAC 
TTCGATGTTA GTGAATCAAT TGTCATCATT GGATTTGGAC AAATGGGTCA GGTTCTGGCC 
AATTTTTTGT CAACGCCATT GGTCTCAGAT AGTGATCTTG TGGGATGGCC TTATATTGGT 
TTTGATCTGA ATCCTGCTGT AGTGAAGGAA TCAAGGAAAC TCGGTTTCCC GATATTGTAT 
GGAGACGGAT CTCGACCATC GGTTTTGCAA TCTGCAGGGG TTTCATCTCC TAAAGCTATC 
ATGATAATGT ATAAAGGCAA GAAGAGAACC ACTGAGGCTG TTCAAAGACT CCGTCTTGCC 
TTCCCCGGGA GTCCTATTTA CGCAAGAGCT CAAGATTTAC CGCATCTCCT TGAACTAAAG 
AAAGCAGGAG CCACAGATGC AATTCTCGAA AATGCAGAGA CAAGTTTACA GCTCGGTTCG 
AAGCTGTTGA CAGGATTTGG AGTAATGTCT GACGACGTAA GCTTTCTAAG TAAAGTTTTC 
AGAGATTCAA TGGAGATACA AGCTCAAGAA GAAATCACAG CTAGTGAAAC AAATGCTGGT 
TTGAAGCCAA TGCAAATGAA AGCATCTGAT ATAAACGTAG TGTCTGCTGC AACACAAAAA 
CAAGTCCAGC TCATGAAGCC AATGCAAATG AAAGCTTCTG ATTCAAACTC AGATTCTGCA 
GCAGAGATTC TTCAAGAAAC AGCTGGTTTG AGTCAGCCTC CTGAGATTGA TGATTCTTCT 
GTGAATATAG ATAATGGATT TGTTGGTAAA GCTGATAAAG CTCAAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190675.1
CDS	511..2841
Translation	MAISTMLGSISCCPSPKGYEMVKQHSVRLKHCVFTVKSSVPVYSEGVNDGIKLHSFGNLVKKKVFLDTSKRFYFQGRWSESSGRRVETYAGVDVASAVDVINDLGFDTLTFLMVTVIIVPAFRILKASPILGFFFAGVVLNQFGLIRNLTDVKVLSEWGILFLLFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHSRPDLVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPLLVILPVLESQDIGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAFVALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFAFVVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDVNFDVSESIVIIGFGQMGQVLANFLSTPLVSDSDLVGWPYIGFDLNPAVVKESRKLGFPILYGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGSPIYARAQDLPHLLELKKAGATDAILENAETSLQLGSKLLTGFGVMSDDVSFLSKVFRDSMEIQAQEEITASETNAGLKPMQMKASDINVVSAATQKQVQLMKPMQMKASDSNSDSAAEILQETAGLSQPPEIDDSSVNIDNGFVGKADKAQD

Target ORF information:

RefSeq Version	NM_001203746.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana K+ efflux antiporter 3 (KEA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203746.1

ORF Insert Sequence:

ATGGCAATTA GTACTATGTT AGGGTCCATC TCTTGTTGCC CCTCTCCCAA GGGGTATGAG 
ATGGTGAAAC AACATAGTGT TCGATTAAAG CATTGCGTTT TCACGGTGAA ATCGAGTGTG 
CCTGTGTATT CGGAAGGCGT AAACGATGGG ATTAAGTTGC ATTCTTTTGG GAATTTAGTT 
AAAAAGAAGG TTTTTCTGGA TACCTCTAAG AGATTCTATT TCCAAGGGAG ATGGTCTGAA 
TCGTCAGGGA GGAGAGTGGA GACTTATGCT GGAGTAGATG TAGCGAGTGC TGTTGATGTA 
ATCAACGATC TTGGATTCGA TACTTTGACC TTCTTGATGG TTACTGTTAT TATTGTCCCT 
GCATTCAGAA TTCTCAAAGC TAGTCCGATA CTTGGTTTCT TCTTTGCTGG TGTTGTACTC 
AACCAATTTG GTTTGATTAG AAATCTCACA GATGTTAAAG TTCTTTCAGA ATGGGGGATT 
CTTTTCCTGC TCTTTGAGAT GGGTCTCGAG CTTTCACTTG CTCGTCTGAA AGCTCTTGCA 
AAATTTGCTT TTGGCATGGG ACTAACTCAG GTTTTGTTAT GCACGGCTGC ATTCACTGCT 
TTTGAACTCC CACCAAATGG AGCCATCGGA ACAAAAATTC TGGAATTCCT CTTTCATTCA 
AGGCCTGACT TGGTCAACAT TAGAAGCATT GATGAGGCCG TTGTCATTGG CGCTGCTCTA 
TCATTGTCAT CTTCAGCTTT TGTTCTACAG CTTCTTGCAG AGAAAGGTGA GCTCCCTACA 
AGATTTGGCT CAGCAACCTT GGGAATTCTT CTTCTACAGG ATATAGCTGT TGTGCCGTTA 
CTAGTCATTC TTCCAGTGTT GGAAAGCCAG GATATTGGTG GGGAAAGCAT ATGGCCAATG 
CTTGCAAAAG AAAGTGCAAA GGCTCTTGGT GGGTTGGGCA TCCTCTCTCT TGGAGGAAAG 
TTCTTTCTCC GTAGAATTTT CGAGGTTGTT GCAGAAACAA GAAGCTCAGA AGCTTTTGTG 
GCCCTTTGCC TTCTCACAGT TGCTGGGACT TCACTTGTGA CTCAATGGCT TGGTTTCAGC 
GATACGCTTG GAGCTTTTCT TGCTGGAGCA CTTCTAGCAG AAACAAATTT CCGAACACAA 
ATTGAAGCTG ATATTAGGCC ATTTAGAGGT TTACTTCTCG GTTTGTTCTT TGTAACCACA 
GGAACTTCCA TTGACATGGA GGTTCTGTTT CGAGAATGGC CTAATGTCCT CTCACTCTTG 
GGTGGTCTGA TTGTGATCAA AACACTGATT ATAACCGCAA TCGGTCCCCG AGTTGGCCTC 
ACAATACAAG AAAGCGTAAG AGTTGGCTTT CTTCTTTCCC AGGGAGGAGA ATTTGCATTT 
GTTGTGTTCT CTCTAGCCAA CAGGCTAGGA GTGCTTCCAA ATGAGCTGAA CAAACTGCTC 
ATTATCGTAG TTGTTTTGTC TATGGCATTA ACACCTTATC TTAACCAACT TGGAAGAAAA 
GCCGCCGATT TTCTTGATGA GAGACTTGAT CCCGGAGAGA AAATAGGTGA AGATGTGAAC 
TTCGATGTTA GTGAATCAAT TGTCATCATT GGATTTGGAC AAATGGGTCA GGTTCTGGCC 
AATTTTTTGT CAACGCCATT GGTCTCAGAT AGTGATCTTG TGGGATGGCC TTATATTGGT 
TTTGATCTGA ATCCTGCTGT AGTGAAGGAA TCAAGGAAAC TCGGTTTCCC GATATTGTAT 
GGAGACGGAT CTCGACCATC GGTTTTGCAA TCTGCAGGGG TTTCATCTCC TAAAGCTATC 
ATGATAATGT ATAAAGGCAA GAAGAGAACC ACTGAGGCTG TTCAAAGACT CCGTCTTGCC 
TTCCCCGGGA GTCCTATTTA CGCAAGAGCT CAAGATTTAC CGCATCTCCT TGAACTAAAG 
AAAGCAGGAG CCACAGATGC AATTCTCGAA AATGCAGAGA CAAGTTTACA GCTCGGTTCG 
AAGCTGTTGA CAGGATTTGG AGTAATGTCT GACGACGTAA GCTTTCTAAG TAAAGTTTTC 
AGAGATTCAA TGGAGATACA AGCTCAAGAA GAAATCACAG CTAGTGAAAC AAATGCTGGT 
TTGAAGCCAA TGCAAATGAA AGCATCTGAT ATAAACGTAG TGTCTGCTGC AACACAAAAA 
CAAGTCCAGC TCATGAAGCC AATGCAAATG AAAGCTTCTG ATTCAAACTC AGATTCTGCA 
GCAGAGATTC TTCAAGAAAC AGCTGGTTTG AGTCAGCCTC CTGAGATTGA TGATTCTTCT 
GTGAATATAG ATAATGGATT TGTTGGTAAA GCTGATAAAG CTCAAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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KEA3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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