EMB2016 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMB2016
Entrez Gene ID	819736
Full Name	embryo defective 2016
Synonyms	F18C1.5,F18C1_5,embryo defective 2016
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001337599.1	NP_001319481.1	embryo defective 2016
NM_111442.7	NP_187219.5	embryo defective 2016

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EMB2016 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMB2016 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb10310	NM_001337599.1 Latest version!	Arabidopsis thaliana embryo defective 2016 (EMB2016), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb10310	NM_111442.7 Latest version!	Arabidopsis thaliana embryo defective 2016 (EMB2016), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMB2016 ( NM_001337599.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001319481.1 Arabidopsis thaliana embryo defective 2016 (EMB2016), mRNA.

Price & Availability↑

CloneID	OAb10310
Clone ID Related Accession (Same CDS sequence)	NM_001337599.1 , NM_111442.7
Accession Version	NM_001337599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	embryo defective 2016
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGTACGAT CTGAGCCTTG TGTTCTGTTC GCTCAGACCT TTGTTCATCC TCAATTGGAC 
GAATATGTTG ACGAGGTGAT ATTTGCTGAG CCAGTAATCA TCACAGCTTG CGAATTTTTG 
GAGCAGAATG CTTCCTCATC TTCTCAAGCT GTTTCTCTTG TCGGGGCAAC TTCACCTCCT 
TCATTTGCGT TGGAAGTGTT CGTTCGATGT GAAGGAGAAT CAAAGTTTAA GCGACTTTGT 
AATCCTTTTC TTTACACTCC TTCAGCGCCC TACCCTTTGG AAGTGGAGGC TGTTGTAACA 
AACCATCTGG TGGTCAGAGG TAGCTATCGA AGTTTGAGCT TGATCGTCTA TGGGAACATC 
GTCAAAGATT TGGGGCAGTA CAATATCATT CTTGAAGGTC GTTCTGTAAC TGATATTGTG 
AGTTCCACTG AGGGTAATCT TGAGGACTTG CCATTGGTAT TGCACTCAGT TAACAGAACA 
ATAGAGGAAT GTCTATCCTC TCTAGACATA GTATCTCTCC CCCTGGCTGC TGTAGATTTG 
CCGGTGGAAG TAAAGCGATT GTTGCAGCTG CTGCTAAAAA TCTTTGATAA GCTAGCTACT 
AATGATGTAG TAAATAAATT TGTGGATACG GTAGTGTCTG GGGTCTCCTC GTATGTCACA 
GACAATGTAG ATTTCTTTTT AAAGAATAAG AATTGTTCGG CTGTGACCAG TTCCCTAGAT 
TCTGGGTTAT TCCATGATAT AGTTGACAGG GTTAAGGAAG ATATTCTTGA TCTCAATGAA 
ATTCAAGAAT CAGATGTGGC ACTTGGATTG TTCAGCTTTC TTGAATCAGA GACTTATTTG 
GCTACTTCCC AACAATTGGT TGTTATGTTG AGCCCATATA TTCAGTTTGA AAGAGATTCC 
TTATGTACTG TCCTTCCAAA ACTTTCAAAG GGGAAAGCCA CTCTTTTAGG TTTAAGTTTG 
GCCTTCTTAT TATGCTCTGG CCGAGAAGGC TGCCTCCAAT TTGTCAATTC GGGTGGGATG 
GATCAACTTG TATATTTGTT TGGTCATGAT GGTCAGAATT CTACCACCAT TACTTTGCTG 
TTGCTTGGAG TTGTTGAGCA GGCTACACGC CATTCAGTTG GGTGTGAAGG ATTTTTAGGT 
TGGTGGCCTC GTGAGGATGG AAGTATCCCA TCTGGTAAGA GTGAGGGCTA CTGTCTTCTG 
TTGAAATTAC TTATGCAGAA ACCATGCCAT GAGATTGCCT CTCTTGCAAT TTATATTCTT 
CGTCGTTTGA GAATTTACGA GGTTATATCA AGATATGAGT TTGCAGTGTT GTCAGCACTG 
GAAGGTCTCT CTAATTCTCA TGGGGCTGCA ACTCATAATC TGAATATGCT ATCTGATGCT 
AAGTCACAAC TTCAAAAGCT CCAGAACCTG ATGAAGTCAC TTGGATCAGT TGAAGATCCC 
TCACCATCAG CCTACGCAGA AAGAAGTTTG GTTTCTGATC ATTCTGAAGG GTGGTTGTCA 
TACAAAGCCA CAAGTAAACT AACTTCTTCA TGGACATGTC CCTTTTATAG CTCTGGAATT 
GATTCGCACA TATTGGCACT TCTGAAGGAG CGGGGTTTTC TTCCTTTGTC AGCAGCGCTT 
CTCTCAATGC CTGAATTGCA TTCTAAAGTT GGAGATATTA TGGATGTCTT TACTGATATC 
GCAATGTTTA TTGGGAACAT AATTCTTTCA TTTATGTTTT CACGCACAGG ATTGAGCTTT 
TTGCTACATC ATCCTGAACT GACTGCTACA ATAATACAAT CTCTGAAGGG ATCCGTCGAT 
CTGAACAAGG AGGAGTGTGT CCCCCTCCAC TATGCTTCAA TTTTAATATC CAAGGGTTTT 
ACTTGCAGCT TACTGGAAAT TGGAATAAAT CTTGAGATGC ATTTGAGGGT GGTGAGTGCA 
GTAGATCGTT TGCTCAAGTC GATACAGCAA ACAGAAGAAT TTCTATGGAT TTTATGGGAG 
TTGCGTGATG TTTCTAGGTC TGATTGTGGG CGTGAAGCTT TATTGACGTT AGGTGTTTTT 
CCAGAGGCTT TAGCAGTCTT GATTGAAGCT TTGCATTCAG CTAAGGATAT GGAGCCGGCA 
GTTGAAAATA GTGGAATTTC ACCGCTTAAT CTAGCAATAT GTCACTCAGC TGCTGAGATT 
TTTGAGGTCA TAGTCTCTGA CTCAACTGCA TCATGCTTGC ATGCTTGGAT TGAACATGCC 
CCAGTCCTTC ATAAGGCCTT GCATACTTTG TCACCGGGTG GTTCTAATCG AAAAGATGCT 
CCTTCACGAT TACTTAAATG GATTGATGCG GGTGTGGTTT ATCACAAACA TGGCGTAGGT 
GGCCTTTTAC GTTATGCTGC TGTCTTGGCT TCTGGAGGGG ACGCCCAATT ATCATCATCA 
AGCATCCTTG CACTCGATTT AACACCGGCT GAGAATGGTG CTGGAGAATC AACAAATGTC 
TCTGAGATGA ACGTCCTAGA TAATCTTGGA AAAGTTATCT TTGAGAAATC TTTTGAAGGT 
GTTAACCTTT CTGATTCATC AATTTCACAG TTGACAACAG CACTTCGAAT TTTAGCGCTG 
ATTTCAGATA ATTCAACTGT TGCTGCTGCT TTATATGACG AGGGTGCGGT TACAGTTGTG 
TATGCTATCT TGGTCAACTG CAGCTTTATG TTTGAGAGGT CGTCAAATAT TTATGATTAT 
CTGGTGGATG ACGATCATGG ATGCAGTTCT ATTTCTGACT TTTTGTCTGA ACGTAATCGT 
GAGCAAAGCT TGGTGGACCT GTTAATTCCT TCCCTTGTCC TCTTGATATC AGTCCTACAA 
AGACTACAGG GAACTAAGGA GCAGTACAGA AACACCAAAT TGATGAAGGC ACTCCTAAGG 
CTACATCGGG AAGTCAGCCC GAAGCTAGCT GCTTGTGCAG CTGACTTGTC CTCCCATTAT 
CCAGACTCTG CTCTTGGCTT TGGAGCAGTG TGCCACCTCA TTGTGTCCGC CCTTGTCTGC 
TGGCCAGTTT ATGGATGGAT TCCTGGGCTA TTCCACACTC TTCTTTCGGG TGTCCAGACG 
TCCTCTGTTC CTGCCTTGGG TCCAAAGGAG ACCTGTAGCT TCCTCTGCAT TCTAAGCGAT 
ATTCTCCCCG AAGAAGGAGT CTGGTTTTGG AAGAGTGGCA TGCCCTTATT AAGTGGTCTA 
AGAAAGTTGG CGGTTGGGAC ATTGATGGGT CCACAGAAGG AGAAACAGAT CAACTGGTAT 
CTGGAGCCTG GTCCACTTGA AAAGCTGATA AACCATTTGA CACCTAATCT TGACAAGATT 
GCAAAGATTA TCCAACATCA TGCAGTATCT GCATTGGTGG TCATTCAGGA TATGCTTCGG 
GTTTTCATTG TTCGTATTGC ATGTCAAAGG GTTGAACATG CTTCTATCCT TCTGAGGCCC 
ATATTTTCAT CTATCAGAGA TGGAATTTTG GATCAGTCAT CAACAAGAGA CACTGAAGCT 
TATATGGTCT ACCGATATCT TAATTTTCTG GCTAGTTTAT TAGAGCATCC GCATGCTAAG 
GGATTACTGT TAGAGGAGGG TATTGTTCAA CTGCTTGTGG AAGTTCTGGA AAGATGTTAT 
GATGCCACTT ATCCAAGTGA AAACAGGGTT CTAGAGTATG GAATTGTGTC CGCGTCTAGT 
GTGATTCAGT GGTGTATTCC AGCTTTCAGA TCGATCTCAC TGCTATGCGA TTCACAAGTG 
CCTCTCTTGT GCTTTCAGAA AAAAGAGCTA TTGGCCAGTC TGAGTGCAAA AGATTGTGCA 
TTGATATTTC CCTTTGTGCT GAAGTTCTGC CAGGTGTTGC CTGTTGGCAA TGAGCTATTA 
TCTTGTCTTG GCGCTTTCAA AGACCTGAGC TCATGTGGTG AAGGTCAAGA TGGTCTGGTT 
TCACTTTTGT TTCATCTGTT TTCTGGTACC GAAGAATCTG TATCTGAAAG GTGGTGTGAC 
ACTAATAGTC TCAGTCTCGA TCAACTAGAT ATGAAGAAAA ATCCCCCTTT TCTGTCTTGC 
TGGATCAAGT TATTGAACTC AATTAATTCA AAAGATGGTT TGTCTAGTCT TGCAATGAAG 
GCAGTAAACG TTCTGAGCGT GGGCTCCATC AGATTATGCC TTGATGGTAA AAGTTTGGAC 
TCGAAGAAGG TTGCAGCACT AAAATCTCTT TTTGGCCTTC CAAGTGAGTT TAGTGGTACA 
GACACTTTTA GAGAGGAAAA CATTGGTTTG ATTGAACAGA TGGTGACACT GTTGAGTTCA 
ATGACTTCGG GGTCTGATAG TTCCGCAACA GCTGAGATGA AACCCTATTT GCATGAGGCA 
TCTCAGTCGT TGCTGTCGTT GCTAAAAGAC GGGAATATTG ATGATATTAT CTCCTGCAAA 
GGTGTTTTTG TTTCTCCTGG GAATCTTGAT ATGGATGATT TGGTTTCTAG AAACATTGAA 
GATGATCTCT ATCAAAGAGG ACTTGAAGAC AAGTTCTGGT GGGAATGTCC AGAAACATTA 
CCTGAGCGTC TGCCCCAGTC ATCTCTCCCT GCGAAAAGGA AACTACCAAC TTTGGAGAGC 
TCTAGCAGGC GTGCTAAAGG CGAAAACTCT TCTGTTGACA TACCTACCCA AAATTCAATT 
CAACGGGGAA TGGGTTCTGT ATCCCTTCCT CCTGCTCCTA CACGTAGAGA TGCCTTTAGG 
CAGCGCAAGC CGAATACCAG CAGGCCTCCC TCTATGCATG TAGATGATTA TGTTGCTAGG 
GAAAGAAGTG TTGATACAGC TGGTAATTCT AATGCAATAA CCATTTCACG AGCAGGATCT 
AGCAGTGGTA GACCTCCATC TATTCATGTT GATGAGTTTA TGGCCAGACA GAGAGAACGA 
GGCCAAAATC CCTCCACCAT TGTGGTTGGA GAGGCTGTTG TGCAAGTGAA AAATCCTACT 
CCTGCTAGAG ATACTGAAAA AGTTGCAGGT AAACCTAAAC AATTCAAAGC CGATCCTGAT 
GATGATCTAC AAGGAATAGA TATAGTTTTT GATGGTGAGG AATGTGAAGG ACCTGATGAC 
AAGTTGCCCT TTCTTCAGCC CGATGAGAAC CTTATGCAGC CTGCTCCTGT CATGGTTGAA 
CAAAACTCTC CTCACTCTAT TGTTGAAGAA ACTGAGAGTG ATGCAAATGG AAGTAGTCAA 
TTCTCTCACA TGGGCACACC AGTGGCATCT AATGTTGACG AAAACGCTCA GAGTGAATTT 
TCATCAAGGA TTTCAGTTTC ACGCCCTGAG ATGTCGTTGA TTCGGGAACC AAGCATTTCT 
TCAGACAGAA AATTTGTTGA GCAAGCAGAC GAAGCAAAGA AAATGGCCCC TCTAAAGAGT 
GCCGGCATAT CTGAATCTGG GTTTATACCT GCTTATCACA TGCCAGGATC TTCAGGACAA 
AATTCAATTG ATCCTAGAGT GGGTCCTCAG GGTTTCTATT CAAAGAGTGG TCAGCAACAT 
ACGGGCCATA TTCATGGTGG ATTCAGTGGT CGGGGGGTTT ATGAACAGAA AGTGATGCCA 
AACCAACCTC CTTTACCCTT AGTGCCACCT CCATCAGTCT CACCTGTGAT ACCACATTCT 
TCTGATTCTC TTTCTAATCA ATCCTCCCCG TTTATTAGTC ATGGAACACA ATCTTCAGGA 
GGACCCACCC GGCTTATGCC ACCGCTCCCT TCAGCAATTC CTCAGTACTC ATCTAACCCA 
TATGCATCTT TACCACCAAG GACATCTACA GTTCAATCTT TTGGATACAA CCACGCAGGC 
GTAGGTACCA CTGAACAACA ACAGAGTGGC CCAACCATTG ATCATCAGTC TGGCAATCTA 
TCTGTTACCG GAATGACGTC TTACCCTCCC CCAAATTTAA TGCCATCCCA TAATTTTAGC 
AGGCCATCAT CTCTCCCTGT GCCATTCTAT GGAAATCCTT CGCATCAAGG AGGTGATAAG 
CCGCAAACTA TGTTGTTGGT TCCTTCCATT CCTCAATCAC TCAATACACA GTCCATCCCT 
CAGCTGCCAT CGATGCAGCT TTCACAATTA CAACGCCCTA TGCAGCCTCC TCAGCACGTT 
AGACCACCCA TACAGATATC TCAACCATCA GAGCAAGGAG TATCAATGCA AAACCCATTT 
CAGATTCCAA TGCATCAGAT GCAGCTGATG CAACAGACGC AAGTCCAGCC TTACTATCAC 
CCTCCTCAAC AACAAGAGAT CTCCCAGGTG CAGCAACAGC AACAGCATCA TGCTGTTCAA 
GGTCAGCAAG GAGCTGGGAC TTCCCAGCAA CAAGAATCTG GAATGTCATT ACATGATTAC 
TTCAAGTCCC CTGAAGCCAT TCAGGCTCTA TTAAGTGACC GTGACAAGCT GTGTCAGCTA 
CTGGAACAAC ATCCAAAGTT AATGCAGATG CTTCAGGAAA AACTTGGGCA GCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001319481.1
CDS	346..6762
Translation	MVRSEPCVLFAQTFVHPQLDEYVDEVIFAEPVIITACEFLEQNASSSSQAVSLVGATSPPSFALEVFVRCEGESKFKRLCNPFLYTPSAPYPLEVEAVVTNHLVVRGSYRSLSLIVYGNIVKDLGQYNIILEGRSVTDIVSSTEGNLEDLPLVLHSVNRTIEECLSSLDIVSLPLAAVDLPVEVKRLLQLLLKIFDKLATNDVVNKFVDTVVSGVSSYVTDNVDFFLKNKNCSAVTSSLDSGLFHDIVDRVKEDILDLNEIQESDVALGLFSFLESETYLATSQQLVVMLSPYIQFERDSLCTVLPKLSKGKATLLGLSLAFLLCSGREGCLQFVNSGGMDQLVYLFGHDGQNSTTITLLLLGVVEQATRHSVGCEGFLGWWPREDGSIPSGKSEGYCLLLKLLMQKPCHEIASLAIYILRRLRIYEVISRYEFAVLSALEGLSNSHGAATHNLNMLSDAKSQLQKLQNLMKSLGSVEDPSPSAYAERSLVSDHSEGWLSYKATSKLTSSWTCPFYSSGIDSHILALLKERGFLPLSAALLSMPELHSKVGDIMDVFTDIAMFIGNIILSFMFSRTGLSFLLHHPELTATIIQSLKGSVDLNKEECVPLHYASILISKGFTCSLLEIGINLEMHLRVVSAVDRLLKSIQQTEEFLWILWELRDVSRSDCGREALLTLGVFPEALAVLIEALHSAKDMEPAVENSGISPLNLAICHSAAEIFEVIVSDSTASCLHAWIEHAPVLHKALHTLSPGGSNRKDAPSRLLKWIDAGVVYHKHGVGGLLRYAAVLASGGDAQLSSSSILALDLTPAENGAGESTNVSEMNVLDNLGKVIFEKSFEGVNLSDSSISQLTTALRILALISDNSTVAAALYDEGAVTVVYAILVNCSFMFERSSNIYDYLVDDDHGCSSISDFLSERNREQSLVDLLIPSLVLLISVLQRLQGTKEQYRNTKLMKALLRLHREVSPKLAACAADLSSHYPDSALGFGAVCHLIVSALVCWPVYGWIPGLFHTLLSGVQTSSVPALGPKETCSFLCILSDILPEEGVWFWKSGMPLLSGLRKLAVGTLMGPQKEKQINWYLEPGPLEKLINHLTPNLDKIAKIIQHHAVSALVVIQDMLRVFIVRIACQRVEHASILLRPIFSSIRDGILDQSSTRDTEAYMVYRYLNFLASLLEHPHAKGLLLEEGIVQLLVEVLERCYDATYPSENRVLEYGIVSASSVIQWCIPAFRSISLLCDSQVPLLCFQKKELLASLSAKDCALIFPFVLKFCQVLPVGNELLSCLGAFKDLSSCGEGQDGLVSLLFHLFSGTEESVSERWCDTNSLSLDQLDMKKNPPFLSCWIKLLNSINSKDGLSSLAMKAVNVLSVGSIRLCLDGKSLDSKKVAALKSLFGLPSEFSGTDTFREENIGLIEQMVTLLSSMTSGSDSSATAEMKPYLHEASQSLLSLLKDGNIDDIISCKGVFVSPGNLDMDDLVSRNIEDDLYQRGLEDKFWWECPETLPERLPQSSLPAKRKLPTLESSSRRAKGENSSVDIPTQNSIQRGMGSVSLPPAPTRRDAFRQRKPNTSRPPSMHVDDYVARERSVDTAGNSNAITISRAGSSSGRPPSIHVDEFMARQRERGQNPSTIVVGEAVVQVKNPTPARDTEKVAGKPKQFKADPDDDLQGIDIVFDGEECEGPDDKLPFLQPDENLMQPAPVMVEQNSPHSIVEETESDANGSSQFSHMGTPVASNVDENAQSEFSSRISVSRPEMSLIREPSISSDRKFVEQADEAKKMAPLKSAGISESGFIPAYHMPGSSGQNSIDPRVGPQGFYSKSGQQHTGHIHGGFSGRGVYEQKVMPNQPPLPLVPPPSVSPVIPHSSDSLSNQSSPFISHGTQSSGGPTRLMPPLPSAIPQYSSNPYASLPPRTSTVQSFGYNHAGVGTTEQQQSGPTIDHQSGNLSVTGMTSYPPPNLMPSHNFSRPSSLPVPFYGNPSHQGGDKPQTMLLVPSIPQSLNTQSIPQLPSMQLSQLQRPMQPPQHVRPPIQISQPSEQGVSMQNPFQIPMHQMQLMQQTQVQPYYHPPQQQEISQVQQQQQHHAVQGQQGAGTSQQQESGMSLHDYFKSPEAIQALLSDRDKLCQLLEQHPKLMQMLQEKLGQL

Target ORF information:

RefSeq Version	NM_001337599.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana embryo defective 2016 (EMB2016), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001337599.1

ORF Insert Sequence:

ATGGTACGAT CTGAGCCTTG TGTTCTGTTC GCTCAGACCT TTGTTCATCC TCAATTGGAC 
GAATATGTTG ACGAGGTGAT ATTTGCTGAG CCAGTAATCA TCACAGCTTG CGAATTTTTG 
GAGCAGAATG CTTCCTCATC TTCTCAAGCT GTTTCTCTTG TCGGGGCAAC TTCACCTCCT 
TCATTTGCGT TGGAAGTGTT CGTTCGATGT GAAGGAGAAT CAAAGTTTAA GCGACTTTGT 
AATCCTTTTC TTTACACTCC TTCAGCGCCC TACCCTTTGG AAGTGGAGGC TGTTGTAACA 
AACCATCTGG TGGTCAGAGG TAGCTATCGA AGTTTGAGCT TGATCGTCTA TGGGAACATC 
GTCAAAGATT TGGGGCAGTA CAATATCATT CTTGAAGGTC GTTCTGTAAC TGATATTGTG 
AGTTCCACTG AGGGTAATCT TGAGGACTTG CCATTGGTAT TGCACTCAGT TAACAGAACA 
ATAGAGGAAT GTCTATCCTC TCTAGACATA GTATCTCTCC CCCTGGCTGC TGTAGATTTG 
CCGGTGGAAG TAAAGCGATT GTTGCAGCTG CTGCTAAAAA TCTTTGATAA GCTAGCTACT 
AATGATGTAG TAAATAAATT TGTGGATACG GTAGTGTCTG GGGTCTCCTC GTATGTCACA 
GACAATGTAG ATTTCTTTTT AAAGAATAAG AATTGTTCGG CTGTGACCAG TTCCCTAGAT 
TCTGGGTTAT TCCATGATAT AGTTGACAGG GTTAAGGAAG ATATTCTTGA TCTCAATGAA 
ATTCAAGAAT CAGATGTGGC ACTTGGATTG TTCAGCTTTC TTGAATCAGA GACTTATTTG 
GCTACTTCCC AACAATTGGT TGTTATGTTG AGCCCATATA TTCAGTTTGA AAGAGATTCC 
TTATGTACTG TCCTTCCAAA ACTTTCAAAG GGGAAAGCCA CTCTTTTAGG TTTAAGTTTG 
GCCTTCTTAT TATGCTCTGG CCGAGAAGGC TGCCTCCAAT TTGTCAATTC GGGTGGGATG 
GATCAACTTG TATATTTGTT TGGTCATGAT GGTCAGAATT CTACCACCAT TACTTTGCTG 
TTGCTTGGAG TTGTTGAGCA GGCTACACGC CATTCAGTTG GGTGTGAAGG ATTTTTAGGT 
TGGTGGCCTC GTGAGGATGG AAGTATCCCA TCTGGTAAGA GTGAGGGCTA CTGTCTTCTG 
TTGAAATTAC TTATGCAGAA ACCATGCCAT GAGATTGCCT CTCTTGCAAT TTATATTCTT 
CGTCGTTTGA GAATTTACGA GGTTATATCA AGATATGAGT TTGCAGTGTT GTCAGCACTG 
GAAGGTCTCT CTAATTCTCA TGGGGCTGCA ACTCATAATC TGAATATGCT ATCTGATGCT 
AAGTCACAAC TTCAAAAGCT CCAGAACCTG ATGAAGTCAC TTGGATCAGT TGAAGATCCC 
TCACCATCAG CCTACGCAGA AAGAAGTTTG GTTTCTGATC ATTCTGAAGG GTGGTTGTCA 
TACAAAGCCA CAAGTAAACT AACTTCTTCA TGGACATGTC CCTTTTATAG CTCTGGAATT 
GATTCGCACA TATTGGCACT TCTGAAGGAG CGGGGTTTTC TTCCTTTGTC AGCAGCGCTT 
CTCTCAATGC CTGAATTGCA TTCTAAAGTT GGAGATATTA TGGATGTCTT TACTGATATC 
GCAATGTTTA TTGGGAACAT AATTCTTTCA TTTATGTTTT CACGCACAGG ATTGAGCTTT 
TTGCTACATC ATCCTGAACT GACTGCTACA ATAATACAAT CTCTGAAGGG ATCCGTCGAT 
CTGAACAAGG AGGAGTGTGT CCCCCTCCAC TATGCTTCAA TTTTAATATC CAAGGGTTTT 
ACTTGCAGCT TACTGGAAAT TGGAATAAAT CTTGAGATGC ATTTGAGGGT GGTGAGTGCA 
GTAGATCGTT TGCTCAAGTC GATACAGCAA ACAGAAGAAT TTCTATGGAT TTTATGGGAG 
TTGCGTGATG TTTCTAGGTC TGATTGTGGG CGTGAAGCTT TATTGACGTT AGGTGTTTTT 
CCAGAGGCTT TAGCAGTCTT GATTGAAGCT TTGCATTCAG CTAAGGATAT GGAGCCGGCA 
GTTGAAAATA GTGGAATTTC ACCGCTTAAT CTAGCAATAT GTCACTCAGC TGCTGAGATT 
TTTGAGGTCA TAGTCTCTGA CTCAACTGCA TCATGCTTGC ATGCTTGGAT TGAACATGCC 
CCAGTCCTTC ATAAGGCCTT GCATACTTTG TCACCGGGTG GTTCTAATCG AAAAGATGCT 
CCTTCACGAT TACTTAAATG GATTGATGCG GGTGTGGTTT ATCACAAACA TGGCGTAGGT 
GGCCTTTTAC GTTATGCTGC TGTCTTGGCT TCTGGAGGGG ACGCCCAATT ATCATCATCA 
AGCATCCTTG CACTCGATTT AACACCGGCT GAGAATGGTG CTGGAGAATC AACAAATGTC 
TCTGAGATGA ACGTCCTAGA TAATCTTGGA AAAGTTATCT TTGAGAAATC TTTTGAAGGT 
GTTAACCTTT CTGATTCATC AATTTCACAG TTGACAACAG CACTTCGAAT TTTAGCGCTG 
ATTTCAGATA ATTCAACTGT TGCTGCTGCT TTATATGACG AGGGTGCGGT TACAGTTGTG 
TATGCTATCT TGGTCAACTG CAGCTTTATG TTTGAGAGGT CGTCAAATAT TTATGATTAT 
CTGGTGGATG ACGATCATGG ATGCAGTTCT ATTTCTGACT TTTTGTCTGA ACGTAATCGT 
GAGCAAAGCT TGGTGGACCT GTTAATTCCT TCCCTTGTCC TCTTGATATC AGTCCTACAA 
AGACTACAGG GAACTAAGGA GCAGTACAGA AACACCAAAT TGATGAAGGC ACTCCTAAGG 
CTACATCGGG AAGTCAGCCC GAAGCTAGCT GCTTGTGCAG CTGACTTGTC CTCCCATTAT 
CCAGACTCTG CTCTTGGCTT TGGAGCAGTG TGCCACCTCA TTGTGTCCGC CCTTGTCTGC 
TGGCCAGTTT ATGGATGGAT TCCTGGGCTA TTCCACACTC TTCTTTCGGG TGTCCAGACG 
TCCTCTGTTC CTGCCTTGGG TCCAAAGGAG ACCTGTAGCT TCCTCTGCAT TCTAAGCGAT 
ATTCTCCCCG AAGAAGGAGT CTGGTTTTGG AAGAGTGGCA TGCCCTTATT AAGTGGTCTA 
AGAAAGTTGG CGGTTGGGAC ATTGATGGGT CCACAGAAGG AGAAACAGAT CAACTGGTAT 
CTGGAGCCTG GTCCACTTGA AAAGCTGATA AACCATTTGA CACCTAATCT TGACAAGATT 
GCAAAGATTA TCCAACATCA TGCAGTATCT GCATTGGTGG TCATTCAGGA TATGCTTCGG 
GTTTTCATTG TTCGTATTGC ATGTCAAAGG GTTGAACATG CTTCTATCCT TCTGAGGCCC 
ATATTTTCAT CTATCAGAGA TGGAATTTTG GATCAGTCAT CAACAAGAGA CACTGAAGCT 
TATATGGTCT ACCGATATCT TAATTTTCTG GCTAGTTTAT TAGAGCATCC GCATGCTAAG 
GGATTACTGT TAGAGGAGGG TATTGTTCAA CTGCTTGTGG AAGTTCTGGA AAGATGTTAT 
GATGCCACTT ATCCAAGTGA AAACAGGGTT CTAGAGTATG GAATTGTGTC CGCGTCTAGT 
GTGATTCAGT GGTGTATTCC AGCTTTCAGA TCGATCTCAC TGCTATGCGA TTCACAAGTG 
CCTCTCTTGT GCTTTCAGAA AAAAGAGCTA TTGGCCAGTC TGAGTGCAAA AGATTGTGCA 
TTGATATTTC CCTTTGTGCT GAAGTTCTGC CAGGTGTTGC CTGTTGGCAA TGAGCTATTA 
TCTTGTCTTG GCGCTTTCAA AGACCTGAGC TCATGTGGTG AAGGTCAAGA TGGTCTGGTT 
TCACTTTTGT TTCATCTGTT TTCTGGTACC GAAGAATCTG TATCTGAAAG GTGGTGTGAC 
ACTAATAGTC TCAGTCTCGA TCAACTAGAT ATGAAGAAAA ATCCCCCTTT TCTGTCTTGC 
TGGATCAAGT TATTGAACTC AATTAATTCA AAAGATGGTT TGTCTAGTCT TGCAATGAAG 
GCAGTAAACG TTCTGAGCGT GGGCTCCATC AGATTATGCC TTGATGGTAA AAGTTTGGAC 
TCGAAGAAGG TTGCAGCACT AAAATCTCTT TTTGGCCTTC CAAGTGAGTT TAGTGGTACA 
GACACTTTTA GAGAGGAAAA CATTGGTTTG ATTGAACAGA TGGTGACACT GTTGAGTTCA 
ATGACTTCGG GGTCTGATAG TTCCGCAACA GCTGAGATGA AACCCTATTT GCATGAGGCA 
TCTCAGTCGT TGCTGTCGTT GCTAAAAGAC GGGAATATTG ATGATATTAT CTCCTGCAAA 
GGTGTTTTTG TTTCTCCTGG GAATCTTGAT ATGGATGATT TGGTTTCTAG AAACATTGAA 
GATGATCTCT ATCAAAGAGG ACTTGAAGAC AAGTTCTGGT GGGAATGTCC AGAAACATTA 
CCTGAGCGTC TGCCCCAGTC ATCTCTCCCT GCGAAAAGGA AACTACCAAC TTTGGAGAGC 
TCTAGCAGGC GTGCTAAAGG CGAAAACTCT TCTGTTGACA TACCTACCCA AAATTCAATT 
CAACGGGGAA TGGGTTCTGT ATCCCTTCCT CCTGCTCCTA CACGTAGAGA TGCCTTTAGG 
CAGCGCAAGC CGAATACCAG CAGGCCTCCC TCTATGCATG TAGATGATTA TGTTGCTAGG 
GAAAGAAGTG TTGATACAGC TGGTAATTCT AATGCAATAA CCATTTCACG AGCAGGATCT 
AGCAGTGGTA GACCTCCATC TATTCATGTT GATGAGTTTA TGGCCAGACA GAGAGAACGA 
GGCCAAAATC CCTCCACCAT TGTGGTTGGA GAGGCTGTTG TGCAAGTGAA AAATCCTACT 
CCTGCTAGAG ATACTGAAAA AGTTGCAGGT AAACCTAAAC AATTCAAAGC CGATCCTGAT 
GATGATCTAC AAGGAATAGA TATAGTTTTT GATGGTGAGG AATGTGAAGG ACCTGATGAC 
AAGTTGCCCT TTCTTCAGCC CGATGAGAAC CTTATGCAGC CTGCTCCTGT CATGGTTGAA 
CAAAACTCTC CTCACTCTAT TGTTGAAGAA ACTGAGAGTG ATGCAAATGG AAGTAGTCAA 
TTCTCTCACA TGGGCACACC AGTGGCATCT AATGTTGACG AAAACGCTCA GAGTGAATTT 
TCATCAAGGA TTTCAGTTTC ACGCCCTGAG ATGTCGTTGA TTCGGGAACC AAGCATTTCT 
TCAGACAGAA AATTTGTTGA GCAAGCAGAC GAAGCAAAGA AAATGGCCCC TCTAAAGAGT 
GCCGGCATAT CTGAATCTGG GTTTATACCT GCTTATCACA TGCCAGGATC TTCAGGACAA 
AATTCAATTG ATCCTAGAGT GGGTCCTCAG GGTTTCTATT CAAAGAGTGG TCAGCAACAT 
ACGGGCCATA TTCATGGTGG ATTCAGTGGT CGGGGGGTTT ATGAACAGAA AGTGATGCCA 
AACCAACCTC CTTTACCCTT AGTGCCACCT CCATCAGTCT CACCTGTGAT ACCACATTCT 
TCTGATTCTC TTTCTAATCA ATCCTCCCCG TTTATTAGTC ATGGAACACA ATCTTCAGGA 
GGACCCACCC GGCTTATGCC ACCGCTCCCT TCAGCAATTC CTCAGTACTC ATCTAACCCA 
TATGCATCTT TACCACCAAG GACATCTACA GTTCAATCTT TTGGATACAA CCACGCAGGC 
GTAGGTACCA CTGAACAACA ACAGAGTGGC CCAACCATTG ATCATCAGTC TGGCAATCTA 
TCTGTTACCG GAATGACGTC TTACCCTCCC CCAAATTTAA TGCCATCCCA TAATTTTAGC 
AGGCCATCAT CTCTCCCTGT GCCATTCTAT GGAAATCCTT CGCATCAAGG AGGTGATAAG 
CCGCAAACTA TGTTGTTGGT TCCTTCCATT CCTCAATCAC TCAATACACA GTCCATCCCT 
CAGCTGCCAT CGATGCAGCT TTCACAATTA CAACGCCCTA TGCAGCCTCC TCAGCACGTT 
AGACCACCCA TACAGATATC TCAACCATCA GAGCAAGGAG TATCAATGCA AAACCCATTT 
CAGATTCCAA TGCATCAGAT GCAGCTGATG CAACAGACGC AAGTCCAGCC TTACTATCAC 
CCTCCTCAAC AACAAGAGAT CTCCCAGGTG CAGCAACAGC AACAGCATCA TGCTGTTCAA 
GGTCAGCAAG GAGCTGGGAC TTCCCAGCAA CAAGAATCTG GAATGTCATT ACATGATTAC 
TTCAAGTCCC CTGAAGCCAT TCAGGCTCTA TTAAGTGACC GTGACAAGCT GTGTCAGCTA 
CTGGAACAAC ATCCAAAGTT AATGCAGATG CTTCAGGAAA AACTTGGGCA GCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMB2016 ( NM_111442.7 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_187219.5 Arabidopsis thaliana embryo defective 2016 (EMB2016), mRNA.

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CloneID	OAb10310
Clone ID Related Accession (Same CDS sequence)	NM_001337599.1 , NM_111442.7
Accession Version	NM_111442.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	embryo defective 2016
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_111442.6.

ATGGTACGAT CTGAGCCTTG TGTTCTGTTC GCTCAGACCT TTGTTCATCC TCAATTGGAC 
GAATATGTTG ACGAGGTGAT ATTTGCTGAG CCAGTAATCA TCACAGCTTG CGAATTTTTG 
GAGCAGAATG CTTCCTCATC TTCTCAAGCT GTTTCTCTTG TCGGGGCAAC TTCACCTCCT 
TCATTTGCGT TGGAAGTGTT CGTTCGATGT GAAGGAGAAT CAAAGTTTAA GCGACTTTGT 
AATCCTTTTC TTTACACTCC TTCAGCGCCC TACCCTTTGG AAGTGGAGGC TGTTGTAACA 
AACCATCTGG TGGTCAGAGG TAGCTATCGA AGTTTGAGCT TGATCGTCTA TGGGAACATC 
GTCAAAGATT TGGGGCAGTA CAATATCATT CTTGAAGGTC GTTCTGTAAC TGATATTGTG 
AGTTCCACTG AGGGTAATCT TGAGGACTTG CCATTGGTAT TGCACTCAGT TAACAGAACA 
ATAGAGGAAT GTCTATCCTC TCTAGACATA GTATCTCTCC CCCTGGCTGC TGTAGATTTG 
CCGGTGGAAG TAAAGCGATT GTTGCAGCTG CTGCTAAAAA TCTTTGATAA GCTAGCTACT 
AATGATGTAG TAAATAAATT TGTGGATACG GTAGTGTCTG GGGTCTCCTC GTATGTCACA 
GACAATGTAG ATTTCTTTTT AAAGAATAAG AATTGTTCGG CTGTGACCAG TTCCCTAGAT 
TCTGGGTTAT TCCATGATAT AGTTGACAGG GTTAAGGAAG ATATTCTTGA TCTCAATGAA 
ATTCAAGAAT CAGATGTGGC ACTTGGATTG TTCAGCTTTC TTGAATCAGA GACTTATTTG 
GCTACTTCCC AACAATTGGT TGTTATGTTG AGCCCATATA TTCAGTTTGA AAGAGATTCC 
TTATGTACTG TCCTTCCAAA ACTTTCAAAG GGGAAAGCCA CTCTTTTAGG TTTAAGTTTG 
GCCTTCTTAT TATGCTCTGG CCGAGAAGGC TGCCTCCAAT TTGTCAATTC GGGTGGGATG 
GATCAACTTG TATATTTGTT TGGTCATGAT GGTCAGAATT CTACCACCAT TACTTTGCTG 
TTGCTTGGAG TTGTTGAGCA GGCTACACGC CATTCAGTTG GGTGTGAAGG ATTTTTAGGT 
TGGTGGCCTC GTGAGGATGG AAGTATCCCA TCTGGTAAGA GTGAGGGCTA CTGTCTTCTG 
TTGAAATTAC TTATGCAGAA ACCATGCCAT GAGATTGCCT CTCTTGCAAT TTATATTCTT 
CGTCGTTTGA GAATTTACGA GGTTATATCA AGATATGAGT TTGCAGTGTT GTCAGCACTG 
GAAGGTCTCT CTAATTCTCA TGGGGCTGCA ACTCATAATC TGAATATGCT ATCTGATGCT 
AAGTCACAAC TTCAAAAGCT CCAGAACCTG ATGAAGTCAC TTGGATCAGT TGAAGATCCC 
TCACCATCAG CCTACGCAGA AAGAAGTTTG GTTTCTGATC ATTCTGAAGG GTGGTTGTCA 
TACAAAGCCA CAAGTAAACT AACTTCTTCA TGGACATGTC CCTTTTATAG CTCTGGAATT 
GATTCGCACA TATTGGCACT TCTGAAGGAG CGGGGTTTTC TTCCTTTGTC AGCAGCGCTT 
CTCTCAATGC CTGAATTGCA TTCTAAAGTT GGAGATATTA TGGATGTCTT TACTGATATC 
GCAATGTTTA TTGGGAACAT AATTCTTTCA TTTATGTTTT CACGCACAGG ATTGAGCTTT 
TTGCTACATC ATCCTGAACT GACTGCTACA ATAATACAAT CTCTGAAGGG ATCCGTCGAT 
CTGAACAAGG AGGAGTGTGT CCCCCTCCAC TATGCTTCAA TTTTAATATC CAAGGGTTTT 
ACTTGCAGCT TACTGGAAAT TGGAATAAAT CTTGAGATGC ATTTGAGGGT GGTGAGTGCA 
GTAGATCGTT TGCTCAAGTC GATACAGCAA ACAGAAGAAT TTCTATGGAT TTTATGGGAG 
TTGCGTGATG TTTCTAGGTC TGATTGTGGG CGTGAAGCTT TATTGACGTT AGGTGTTTTT 
CCAGAGGCTT TAGCAGTCTT GATTGAAGCT TTGCATTCAG CTAAGGATAT GGAGCCGGCA 
GTTGAAAATA GTGGAATTTC ACCGCTTAAT CTAGCAATAT GTCACTCAGC TGCTGAGATT 
TTTGAGGTCA TAGTCTCTGA CTCAACTGCA TCATGCTTGC ATGCTTGGAT TGAACATGCC 
CCAGTCCTTC ATAAGGCCTT GCATACTTTG TCACCGGGTG GTTCTAATCG AAAAGATGCT 
CCTTCACGAT TACTTAAATG GATTGATGCG GGTGTGGTTT ATCACAAACA TGGCGTAGGT 
GGCCTTTTAC GTTATGCTGC TGTCTTGGCT TCTGGAGGGG ACGCCCAATT ATCATCATCA 
AGCATCCTTG CACTCGATTT AACACCGGCT GAGAATGGTG CTGGAGAATC AACAAATGTC 
TCTGAGATGA ACGTCCTAGA TAATCTTGGA AAAGTTATCT TTGAGAAATC TTTTGAAGGT 
GTTAACCTTT CTGATTCATC AATTTCACAG TTGACAACAG CACTTCGAAT TTTAGCGCTG 
ATTTCAGATA ATTCAACTGT TGCTGCTGCT TTATATGACG AGGGTGCGGT TACAGTTGTG 
TATGCTATCT TGGTCAACTG CAGCTTTATG TTTGAGAGGT CGTCAAATAT TTATGATTAT 
CTGGTGGATG ACGATCATGG ATGCAGTTCT ATTTCTGACT TTTTGTCTGA ACGTAATCGT 
GAGCAAAGCT TGGTGGACCT GTTAATTCCT TCCCTTGTCC TCTTGATATC AGTCCTACAA 
AGACTACAGG GAACTAAGGA GCAGTACAGA AACACCAAAT TGATGAAGGC ACTCCTAAGG 
CTACATCGGG AAGTCAGCCC GAAGCTAGCT GCTTGTGCAG CTGACTTGTC CTCCCATTAT 
CCAGACTCTG CTCTTGGCTT TGGAGCAGTG TGCCACCTCA TTGTGTCCGC CCTTGTCTGC 
TGGCCAGTTT ATGGATGGAT TCCTGGGCTA TTCCACACTC TTCTTTCGGG TGTCCAGACG 
TCCTCTGTTC CTGCCTTGGG TCCAAAGGAG ACCTGTAGCT TCCTCTGCAT TCTAAGCGAT 
ATTCTCCCCG AAGAAGGAGT CTGGTTTTGG AAGAGTGGCA TGCCCTTATT AAGTGGTCTA 
AGAAAGTTGG CGGTTGGGAC ATTGATGGGT CCACAGAAGG AGAAACAGAT CAACTGGTAT 
CTGGAGCCTG GTCCACTTGA AAAGCTGATA AACCATTTGA CACCTAATCT TGACAAGATT 
GCAAAGATTA TCCAACATCA TGCAGTATCT GCATTGGTGG TCATTCAGGA TATGCTTCGG 
GTTTTCATTG TTCGTATTGC ATGTCAAAGG GTTGAACATG CTTCTATCCT TCTGAGGCCC 
ATATTTTCAT CTATCAGAGA TGGAATTTTG GATCAGTCAT CAACAAGAGA CACTGAAGCT 
TATATGGTCT ACCGATATCT TAATTTTCTG GCTAGTTTAT TAGAGCATCC GCATGCTAAG 
GGATTACTGT TAGAGGAGGG TATTGTTCAA CTGCTTGTGG AAGTTCTGGA AAGATGTTAT 
GATGCCACTT ATCCAAGTGA AAACAGGGTT CTAGAGTATG GAATTGTGTC CGCGTCTAGT 
GTGATTCAGT GGTGTATTCC AGCTTTCAGA TCGATCTCAC TGCTATGCGA TTCACAAGTG 
CCTCTCTTGT GCTTTCAGAA AAAAGAGCTA TTGGCCAGTC TGAGTGCAAA AGATTGTGCA 
TTGATATTTC CCTTTGTGCT GAAGTTCTGC CAGGTGTTGC CTGTTGGCAA TGAGCTATTA 
TCTTGTCTTG GCGCTTTCAA AGACCTGAGC TCATGTGGTG AAGGTCAAGA TGGTCTGGTT 
TCACTTTTGT TTCATCTGTT TTCTGGTACC GAAGAATCTG TATCTGAAAG GTGGTGTGAC 
ACTAATAGTC TCAGTCTCGA TCAACTAGAT ATGAAGAAAA ATCCCCCTTT TCTGTCTTGC 
TGGATCAAGT TATTGAACTC AATTAATTCA AAAGATGGTT TGTCTAGTCT TGCAATGAAG 
GCAGTAAACG TTCTGAGCGT GGGCTCCATC AGATTATGCC TTGATGGTAA AAGTTTGGAC 
TCGAAGAAGG TTGCAGCACT AAAATCTCTT TTTGGCCTTC CAAGTGAGTT TAGTGGTACA 
GACACTTTTA GAGAGGAAAA CATTGGTTTG ATTGAACAGA TGGTGACACT GTTGAGTTCA 
ATGACTTCGG GGTCTGATAG TTCCGCAACA GCTGAGATGA AACCCTATTT GCATGAGGCA 
TCTCAGTCGT TGCTGTCGTT GCTAAAAGAC GGGAATATTG ATGATATTAT CTCCTGCAAA 
GGTGTTTTTG TTTCTCCTGG GAATCTTGAT ATGGATGATT TGGTTTCTAG AAACATTGAA 
GATGATCTCT ATCAAAGAGG ACTTGAAGAC AAGTTCTGGT GGGAATGTCC AGAAACATTA 
CCTGAGCGTC TGCCCCAGTC ATCTCTCCCT GCGAAAAGGA AACTACCAAC TTTGGAGAGC 
TCTAGCAGGC GTGCTAAAGG CGAAAACTCT TCTGTTGACA TACCTACCCA AAATTCAATT 
CAACGGGGAA TGGGTTCTGT ATCCCTTCCT CCTGCTCCTA CACGTAGAGA TGCCTTTAGG 
CAGCGCAAGC CGAATACCAG CAGGCCTCCC TCTATGCATG TAGATGATTA TGTTGCTAGG 
GAAAGAAGTG TTGATACAGC TGGTAATTCT AATGCAATAA CCATTTCACG AGCAGGATCT 
AGCAGTGGTA GACCTCCATC TATTCATGTT GATGAGTTTA TGGCCAGACA GAGAGAACGA 
GGCCAAAATC CCTCCACCAT TGTGGTTGGA GAGGCTGTTG TGCAAGTGAA AAATCCTACT 
CCTGCTAGAG ATACTGAAAA AGTTGCAGGT AAACCTAAAC AATTCAAAGC CGATCCTGAT 
GATGATCTAC AAGGAATAGA TATAGTTTTT GATGGTGAGG AATGTGAAGG ACCTGATGAC 
AAGTTGCCCT TTCTTCAGCC CGATGAGAAC CTTATGCAGC CTGCTCCTGT CATGGTTGAA 
CAAAACTCTC CTCACTCTAT TGTTGAAGAA ACTGAGAGTG ATGCAAATGG AAGTAGTCAA 
TTCTCTCACA TGGGCACACC AGTGGCATCT AATGTTGACG AAAACGCTCA GAGTGAATTT 
TCATCAAGGA TTTCAGTTTC ACGCCCTGAG ATGTCGTTGA TTCGGGAACC AAGCATTTCT 
TCAGACAGAA AATTTGTTGA GCAAGCAGAC GAAGCAAAGA AAATGGCCCC TCTAAAGAGT 
GCCGGCATAT CTGAATCTGG GTTTATACCT GCTTATCACA TGCCAGGATC TTCAGGACAA 
AATTCAATTG ATCCTAGAGT GGGTCCTCAG GGTTTCTATT CAAAGAGTGG TCAGCAACAT 
ACGGGCCATA TTCATGGTGG ATTCAGTGGT CGGGGGGTTT ATGAACAGAA AGTGATGCCA 
AACCAACCTC CTTTACCCTT AGTGCCACCT CCATCAGTCT CACCTGTGAT ACCACATTCT 
TCTGATTCTC TTTCTAATCA ATCCTCCCCG TTTATTAGTC ATGGAACACA ATCTTCAGGA 
GGACCCACCC GGCTTATGCC ACCGCTCCCT TCAGCAATTC CTCAGTACTC ATCTAACCCA 
TATGCATCTT TACCACCAAG GACATCTACA GTTCAATCTT TTGGATACAA CCACGCAGGC 
GTAGGTACCA CTGAACAACA ACAGAGTGGC CCAACCATTG ATCATCAGTC TGGCAATCTA 
TCTGTTACCG GAATGACGTC TTACCCTCCC CCAAATTTAA TGCCATCCCA TAATTTTAGC 
AGGCCATCAT CTCTCCCTGT GCCATTCTAT GGAAATCCTT CGCATCAAGG AGGTGATAAG 
CCGCAAACTA TGTTGTTGGT TCCTTCCATT CCTCAATCAC TCAATACACA GTCCATCCCT 
CAGCTGCCAT CGATGCAGCT TTCACAATTA CAACGCCCTA TGCAGCCTCC TCAGCACGTT 
AGACCACCCA TACAGATATC TCAACCATCA GAGCAAGGAG TATCAATGCA AAACCCATTT 
CAGATTCCAA TGCATCAGAT GCAGCTGATG CAACAGACGC AAGTCCAGCC TTACTATCAC 
CCTCCTCAAC AACAAGAGAT CTCCCAGGTG CAGCAACAGC AACAGCATCA TGCTGTTCAA 
GGTCAGCAAG GAGCTGGGAC TTCCCAGCAA CAAGAATCTG GAATGTCATT ACATGATTAC 
TTCAAGTCCC CTGAAGCCAT TCAGGCTCTA TTAAGTGACC GTGACAAGCT GTGTCAGCTA 
CTGGAACAAC ATCCAAAGTT AATGCAGATG CTTCAGGAAA AACTTGGGCA GCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_187219.5
CDS	253..6669
Translation	MVRSEPCVLFAQTFVHPQLDEYVDEVIFAEPVIITACEFLEQNASSSSQAVSLVGATSPPSFALEVFVRCEGESKFKRLCNPFLYTPSAPYPLEVEAVVTNHLVVRGSYRSLSLIVYGNIVKDLGQYNIILEGRSVTDIVSSTEGNLEDLPLVLHSVNRTIEECLSSLDIVSLPLAAVDLPVEVKRLLQLLLKIFDKLATNDVVNKFVDTVVSGVSSYVTDNVDFFLKNKNCSAVTSSLDSGLFHDIVDRVKEDILDLNEIQESDVALGLFSFLESETYLATSQQLVVMLSPYIQFERDSLCTVLPKLSKGKATLLGLSLAFLLCSGREGCLQFVNSGGMDQLVYLFGHDGQNSTTITLLLLGVVEQATRHSVGCEGFLGWWPREDGSIPSGKSEGYCLLLKLLMQKPCHEIASLAIYILRRLRIYEVISRYEFAVLSALEGLSNSHGAATHNLNMLSDAKSQLQKLQNLMKSLGSVEDPSPSAYAERSLVSDHSEGWLSYKATSKLTSSWTCPFYSSGIDSHILALLKERGFLPLSAALLSMPELHSKVGDIMDVFTDIAMFIGNIILSFMFSRTGLSFLLHHPELTATIIQSLKGSVDLNKEECVPLHYASILISKGFTCSLLEIGINLEMHLRVVSAVDRLLKSIQQTEEFLWILWELRDVSRSDCGREALLTLGVFPEALAVLIEALHSAKDMEPAVENSGISPLNLAICHSAAEIFEVIVSDSTASCLHAWIEHAPVLHKALHTLSPGGSNRKDAPSRLLKWIDAGVVYHKHGVGGLLRYAAVLASGGDAQLSSSSILALDLTPAENGAGESTNVSEMNVLDNLGKVIFEKSFEGVNLSDSSISQLTTALRILALISDNSTVAAALYDEGAVTVVYAILVNCSFMFERSSNIYDYLVDDDHGCSSISDFLSERNREQSLVDLLIPSLVLLISVLQRLQGTKEQYRNTKLMKALLRLHREVSPKLAACAADLSSHYPDSALGFGAVCHLIVSALVCWPVYGWIPGLFHTLLSGVQTSSVPALGPKETCSFLCILSDILPEEGVWFWKSGMPLLSGLRKLAVGTLMGPQKEKQINWYLEPGPLEKLINHLTPNLDKIAKIIQHHAVSALVVIQDMLRVFIVRIACQRVEHASILLRPIFSSIRDGILDQSSTRDTEAYMVYRYLNFLASLLEHPHAKGLLLEEGIVQLLVEVLERCYDATYPSENRVLEYGIVSASSVIQWCIPAFRSISLLCDSQVPLLCFQKKELLASLSAKDCALIFPFVLKFCQVLPVGNELLSCLGAFKDLSSCGEGQDGLVSLLFHLFSGTEESVSERWCDTNSLSLDQLDMKKNPPFLSCWIKLLNSINSKDGLSSLAMKAVNVLSVGSIRLCLDGKSLDSKKVAALKSLFGLPSEFSGTDTFREENIGLIEQMVTLLSSMTSGSDSSATAEMKPYLHEASQSLLSLLKDGNIDDIISCKGVFVSPGNLDMDDLVSRNIEDDLYQRGLEDKFWWECPETLPERLPQSSLPAKRKLPTLESSSRRAKGENSSVDIPTQNSIQRGMGSVSLPPAPTRRDAFRQRKPNTSRPPSMHVDDYVARERSVDTAGNSNAITISRAGSSSGRPPSIHVDEFMARQRERGQNPSTIVVGEAVVQVKNPTPARDTEKVAGKPKQFKADPDDDLQGIDIVFDGEECEGPDDKLPFLQPDENLMQPAPVMVEQNSPHSIVEETESDANGSSQFSHMGTPVASNVDENAQSEFSSRISVSRPEMSLIREPSISSDRKFVEQADEAKKMAPLKSAGISESGFIPAYHMPGSSGQNSIDPRVGPQGFYSKSGQQHTGHIHGGFSGRGVYEQKVMPNQPPLPLVPPPSVSPVIPHSSDSLSNQSSPFISHGTQSSGGPTRLMPPLPSAIPQYSSNPYASLPPRTSTVQSFGYNHAGVGTTEQQQSGPTIDHQSGNLSVTGMTSYPPPNLMPSHNFSRPSSLPVPFYGNPSHQGGDKPQTMLLVPSIPQSLNTQSIPQLPSMQLSQLQRPMQPPQHVRPPIQISQPSEQGVSMQNPFQIPMHQMQLMQQTQVQPYYHPPQQQEISQVQQQQQHHAVQGQQGAGTSQQQESGMSLHDYFKSPEAIQALLSDRDKLCQLLEQHPKLMQMLQEKLGQL

Target ORF information:

RefSeq Version	NM_111442.7
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana embryo defective 2016 (EMB2016), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_111442.7

ORF Insert Sequence:

ATGGTACGAT CTGAGCCTTG TGTTCTGTTC GCTCAGACCT TTGTTCATCC TCAATTGGAC 
GAATATGTTG ACGAGGTGAT ATTTGCTGAG CCAGTAATCA TCACAGCTTG CGAATTTTTG 
GAGCAGAATG CTTCCTCATC TTCTCAAGCT GTTTCTCTTG TCGGGGCAAC TTCACCTCCT 
TCATTTGCGT TGGAAGTGTT CGTTCGATGT GAAGGAGAAT CAAAGTTTAA GCGACTTTGT 
AATCCTTTTC TTTACACTCC TTCAGCGCCC TACCCTTTGG AAGTGGAGGC TGTTGTAACA 
AACCATCTGG TGGTCAGAGG TAGCTATCGA AGTTTGAGCT TGATCGTCTA TGGGAACATC 
GTCAAAGATT TGGGGCAGTA CAATATCATT CTTGAAGGTC GTTCTGTAAC TGATATTGTG 
AGTTCCACTG AGGGTAATCT TGAGGACTTG CCATTGGTAT TGCACTCAGT TAACAGAACA 
ATAGAGGAAT GTCTATCCTC TCTAGACATA GTATCTCTCC CCCTGGCTGC TGTAGATTTG 
CCGGTGGAAG TAAAGCGATT GTTGCAGCTG CTGCTAAAAA TCTTTGATAA GCTAGCTACT 
AATGATGTAG TAAATAAATT TGTGGATACG GTAGTGTCTG GGGTCTCCTC GTATGTCACA 
GACAATGTAG ATTTCTTTTT AAAGAATAAG AATTGTTCGG CTGTGACCAG TTCCCTAGAT 
TCTGGGTTAT TCCATGATAT AGTTGACAGG GTTAAGGAAG ATATTCTTGA TCTCAATGAA 
ATTCAAGAAT CAGATGTGGC ACTTGGATTG TTCAGCTTTC TTGAATCAGA GACTTATTTG 
GCTACTTCCC AACAATTGGT TGTTATGTTG AGCCCATATA TTCAGTTTGA AAGAGATTCC 
TTATGTACTG TCCTTCCAAA ACTTTCAAAG GGGAAAGCCA CTCTTTTAGG TTTAAGTTTG 
GCCTTCTTAT TATGCTCTGG CCGAGAAGGC TGCCTCCAAT TTGTCAATTC GGGTGGGATG 
GATCAACTTG TATATTTGTT TGGTCATGAT GGTCAGAATT CTACCACCAT TACTTTGCTG 
TTGCTTGGAG TTGTTGAGCA GGCTACACGC CATTCAGTTG GGTGTGAAGG ATTTTTAGGT 
TGGTGGCCTC GTGAGGATGG AAGTATCCCA TCTGGTAAGA GTGAGGGCTA CTGTCTTCTG 
TTGAAATTAC TTATGCAGAA ACCATGCCAT GAGATTGCCT CTCTTGCAAT TTATATTCTT 
CGTCGTTTGA GAATTTACGA GGTTATATCA AGATATGAGT TTGCAGTGTT GTCAGCACTG 
GAAGGTCTCT CTAATTCTCA TGGGGCTGCA ACTCATAATC TGAATATGCT ATCTGATGCT 
AAGTCACAAC TTCAAAAGCT CCAGAACCTG ATGAAGTCAC TTGGATCAGT TGAAGATCCC 
TCACCATCAG CCTACGCAGA AAGAAGTTTG GTTTCTGATC ATTCTGAAGG GTGGTTGTCA 
TACAAAGCCA CAAGTAAACT AACTTCTTCA TGGACATGTC CCTTTTATAG CTCTGGAATT 
GATTCGCACA TATTGGCACT TCTGAAGGAG CGGGGTTTTC TTCCTTTGTC AGCAGCGCTT 
CTCTCAATGC CTGAATTGCA TTCTAAAGTT GGAGATATTA TGGATGTCTT TACTGATATC 
GCAATGTTTA TTGGGAACAT AATTCTTTCA TTTATGTTTT CACGCACAGG ATTGAGCTTT 
TTGCTACATC ATCCTGAACT GACTGCTACA ATAATACAAT CTCTGAAGGG ATCCGTCGAT 
CTGAACAAGG AGGAGTGTGT CCCCCTCCAC TATGCTTCAA TTTTAATATC CAAGGGTTTT 
ACTTGCAGCT TACTGGAAAT TGGAATAAAT CTTGAGATGC ATTTGAGGGT GGTGAGTGCA 
GTAGATCGTT TGCTCAAGTC GATACAGCAA ACAGAAGAAT TTCTATGGAT TTTATGGGAG 
TTGCGTGATG TTTCTAGGTC TGATTGTGGG CGTGAAGCTT TATTGACGTT AGGTGTTTTT 
CCAGAGGCTT TAGCAGTCTT GATTGAAGCT TTGCATTCAG CTAAGGATAT GGAGCCGGCA 
GTTGAAAATA GTGGAATTTC ACCGCTTAAT CTAGCAATAT GTCACTCAGC TGCTGAGATT 
TTTGAGGTCA TAGTCTCTGA CTCAACTGCA TCATGCTTGC ATGCTTGGAT TGAACATGCC 
CCAGTCCTTC ATAAGGCCTT GCATACTTTG TCACCGGGTG GTTCTAATCG AAAAGATGCT 
CCTTCACGAT TACTTAAATG GATTGATGCG GGTGTGGTTT ATCACAAACA TGGCGTAGGT 
GGCCTTTTAC GTTATGCTGC TGTCTTGGCT TCTGGAGGGG ACGCCCAATT ATCATCATCA 
AGCATCCTTG CACTCGATTT AACACCGGCT GAGAATGGTG CTGGAGAATC AACAAATGTC 
TCTGAGATGA ACGTCCTAGA TAATCTTGGA AAAGTTATCT TTGAGAAATC TTTTGAAGGT 
GTTAACCTTT CTGATTCATC AATTTCACAG TTGACAACAG CACTTCGAAT TTTAGCGCTG 
ATTTCAGATA ATTCAACTGT TGCTGCTGCT TTATATGACG AGGGTGCGGT TACAGTTGTG 
TATGCTATCT TGGTCAACTG CAGCTTTATG TTTGAGAGGT CGTCAAATAT TTATGATTAT 
CTGGTGGATG ACGATCATGG ATGCAGTTCT ATTTCTGACT TTTTGTCTGA ACGTAATCGT 
GAGCAAAGCT TGGTGGACCT GTTAATTCCT TCCCTTGTCC TCTTGATATC AGTCCTACAA 
AGACTACAGG GAACTAAGGA GCAGTACAGA AACACCAAAT TGATGAAGGC ACTCCTAAGG 
CTACATCGGG AAGTCAGCCC GAAGCTAGCT GCTTGTGCAG CTGACTTGTC CTCCCATTAT 
CCAGACTCTG CTCTTGGCTT TGGAGCAGTG TGCCACCTCA TTGTGTCCGC CCTTGTCTGC 
TGGCCAGTTT ATGGATGGAT TCCTGGGCTA TTCCACACTC TTCTTTCGGG TGTCCAGACG 
TCCTCTGTTC CTGCCTTGGG TCCAAAGGAG ACCTGTAGCT TCCTCTGCAT TCTAAGCGAT 
ATTCTCCCCG AAGAAGGAGT CTGGTTTTGG AAGAGTGGCA TGCCCTTATT AAGTGGTCTA 
AGAAAGTTGG CGGTTGGGAC ATTGATGGGT CCACAGAAGG AGAAACAGAT CAACTGGTAT 
CTGGAGCCTG GTCCACTTGA AAAGCTGATA AACCATTTGA CACCTAATCT TGACAAGATT 
GCAAAGATTA TCCAACATCA TGCAGTATCT GCATTGGTGG TCATTCAGGA TATGCTTCGG 
GTTTTCATTG TTCGTATTGC ATGTCAAAGG GTTGAACATG CTTCTATCCT TCTGAGGCCC 
ATATTTTCAT CTATCAGAGA TGGAATTTTG GATCAGTCAT CAACAAGAGA CACTGAAGCT 
TATATGGTCT ACCGATATCT TAATTTTCTG GCTAGTTTAT TAGAGCATCC GCATGCTAAG 
GGATTACTGT TAGAGGAGGG TATTGTTCAA CTGCTTGTGG AAGTTCTGGA AAGATGTTAT 
GATGCCACTT ATCCAAGTGA AAACAGGGTT CTAGAGTATG GAATTGTGTC CGCGTCTAGT 
GTGATTCAGT GGTGTATTCC AGCTTTCAGA TCGATCTCAC TGCTATGCGA TTCACAAGTG 
CCTCTCTTGT GCTTTCAGAA AAAAGAGCTA TTGGCCAGTC TGAGTGCAAA AGATTGTGCA 
TTGATATTTC CCTTTGTGCT GAAGTTCTGC CAGGTGTTGC CTGTTGGCAA TGAGCTATTA 
TCTTGTCTTG GCGCTTTCAA AGACCTGAGC TCATGTGGTG AAGGTCAAGA TGGTCTGGTT 
TCACTTTTGT TTCATCTGTT TTCTGGTACC GAAGAATCTG TATCTGAAAG GTGGTGTGAC 
ACTAATAGTC TCAGTCTCGA TCAACTAGAT ATGAAGAAAA ATCCCCCTTT TCTGTCTTGC 
TGGATCAAGT TATTGAACTC AATTAATTCA AAAGATGGTT TGTCTAGTCT TGCAATGAAG 
GCAGTAAACG TTCTGAGCGT GGGCTCCATC AGATTATGCC TTGATGGTAA AAGTTTGGAC 
TCGAAGAAGG TTGCAGCACT AAAATCTCTT TTTGGCCTTC CAAGTGAGTT TAGTGGTACA 
GACACTTTTA GAGAGGAAAA CATTGGTTTG ATTGAACAGA TGGTGACACT GTTGAGTTCA 
ATGACTTCGG GGTCTGATAG TTCCGCAACA GCTGAGATGA AACCCTATTT GCATGAGGCA 
TCTCAGTCGT TGCTGTCGTT GCTAAAAGAC GGGAATATTG ATGATATTAT CTCCTGCAAA 
GGTGTTTTTG TTTCTCCTGG GAATCTTGAT ATGGATGATT TGGTTTCTAG AAACATTGAA 
GATGATCTCT ATCAAAGAGG ACTTGAAGAC AAGTTCTGGT GGGAATGTCC AGAAACATTA 
CCTGAGCGTC TGCCCCAGTC ATCTCTCCCT GCGAAAAGGA AACTACCAAC TTTGGAGAGC 
TCTAGCAGGC GTGCTAAAGG CGAAAACTCT TCTGTTGACA TACCTACCCA AAATTCAATT 
CAACGGGGAA TGGGTTCTGT ATCCCTTCCT CCTGCTCCTA CACGTAGAGA TGCCTTTAGG 
CAGCGCAAGC CGAATACCAG CAGGCCTCCC TCTATGCATG TAGATGATTA TGTTGCTAGG 
GAAAGAAGTG TTGATACAGC TGGTAATTCT AATGCAATAA CCATTTCACG AGCAGGATCT 
AGCAGTGGTA GACCTCCATC TATTCATGTT GATGAGTTTA TGGCCAGACA GAGAGAACGA 
GGCCAAAATC CCTCCACCAT TGTGGTTGGA GAGGCTGTTG TGCAAGTGAA AAATCCTACT 
CCTGCTAGAG ATACTGAAAA AGTTGCAGGT AAACCTAAAC AATTCAAAGC CGATCCTGAT 
GATGATCTAC AAGGAATAGA TATAGTTTTT GATGGTGAGG AATGTGAAGG ACCTGATGAC 
AAGTTGCCCT TTCTTCAGCC CGATGAGAAC CTTATGCAGC CTGCTCCTGT CATGGTTGAA 
CAAAACTCTC CTCACTCTAT TGTTGAAGAA ACTGAGAGTG ATGCAAATGG AAGTAGTCAA 
TTCTCTCACA TGGGCACACC AGTGGCATCT AATGTTGACG AAAACGCTCA GAGTGAATTT 
TCATCAAGGA TTTCAGTTTC ACGCCCTGAG ATGTCGTTGA TTCGGGAACC AAGCATTTCT 
TCAGACAGAA AATTTGTTGA GCAAGCAGAC GAAGCAAAGA AAATGGCCCC TCTAAAGAGT 
GCCGGCATAT CTGAATCTGG GTTTATACCT GCTTATCACA TGCCAGGATC TTCAGGACAA 
AATTCAATTG ATCCTAGAGT GGGTCCTCAG GGTTTCTATT CAAAGAGTGG TCAGCAACAT 
ACGGGCCATA TTCATGGTGG ATTCAGTGGT CGGGGGGTTT ATGAACAGAA AGTGATGCCA 
AACCAACCTC CTTTACCCTT AGTGCCACCT CCATCAGTCT CACCTGTGAT ACCACATTCT 
TCTGATTCTC TTTCTAATCA ATCCTCCCCG TTTATTAGTC ATGGAACACA ATCTTCAGGA 
GGACCCACCC GGCTTATGCC ACCGCTCCCT TCAGCAATTC CTCAGTACTC ATCTAACCCA 
TATGCATCTT TACCACCAAG GACATCTACA GTTCAATCTT TTGGATACAA CCACGCAGGC 
GTAGGTACCA CTGAACAACA ACAGAGTGGC CCAACCATTG ATCATCAGTC TGGCAATCTA 
TCTGTTACCG GAATGACGTC TTACCCTCCC CCAAATTTAA TGCCATCCCA TAATTTTAGC 
AGGCCATCAT CTCTCCCTGT GCCATTCTAT GGAAATCCTT CGCATCAAGG AGGTGATAAG 
CCGCAAACTA TGTTGTTGGT TCCTTCCATT CCTCAATCAC TCAATACACA GTCCATCCCT 
CAGCTGCCAT CGATGCAGCT TTCACAATTA CAACGCCCTA TGCAGCCTCC TCAGCACGTT 
AGACCACCCA TACAGATATC TCAACCATCA GAGCAAGGAG TATCAATGCA AAACCCATTT 
CAGATTCCAA TGCATCAGAT GCAGCTGATG CAACAGACGC AAGTCCAGCC TTACTATCAC 
CCTCCTCAAC AACAAGAGAT CTCCCAGGTG CAGCAACAGC AACAGCATCA TGCTGTTCAA 
GGTCAGCAAG GAGCTGGGAC TTCCCAGCAA CAAGAATCTG GAATGTCATT ACATGATTAC 
TTCAAGTCCC CTGAAGCCAT TCAGGCTCTA TTAAGTGACC GTGACAAGCT GTGTCAGCTA 
CTGGAACAAC ATCCAAAGTT AATGCAGATG CTTCAGGAAA AACTTGGGCA GCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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EMB2016 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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