emb2458 cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following emb2458 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the emb2458 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb10165 NM_111305.7
Latest version!
Arabidopsis thaliana FtsH extracellular protease family (emb2458), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb10165
    Clone ID Related Accession (Same CDS sequence) NM_111305.7
    Accession Version NM_111305.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product FtsH extracellular protease family
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_111305.6.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATTTCA TTTCCGCTTC TTCCTTATCT TCACCCTTTT CCACACAGTT GTCTCCGATT 
    TATTTGAGCT CAGGTATAGT ATCGTTAAAG CCTAGACATC GAGTCAAAAA TCGAAACTTT
    GGGTCTAGAG AATCCAATAA TAAATCTAGG AAAATTGTGC CCATTAGAGG CTGCTTTGGT
    TTTTCCGGGA GTTTTTTGAG GTCGAAACAG TCTGATTACG GTAGTGAAGC TGTTTCGGAA
    TCATTGAGAT TATGTGGAGA GGGAAATGAG CTTGTGCTAT CTTCCGAGTA TAATAGCGCA
    AAGACGAGAG AGAGCGTAAT ACAGTTTGTT ACCAAGCCTC TAGTTTACGC CTTGTTTTGT
    ATTGCAATTG GACTTTCTCC GATTCGCTCT TTTCAAGCTC CAGCTTTAGC TGTGCCTTTC
    GTCAGTGATG TTATTTGGAA GAAAAAGAAA GAGAGAGTGA GAGAGAAAGA GGTGGTTTTG
    AAAGCAGTTG ATCATGAGTT CTCGGATTAT ACTCGGAGAT TGTTGGAGAC AGTCTCGGTT
    TTACTGAAGA CTATTGAGAT AGTTAGAAAG GAAAATGGAG AGGTTGCTGA AGTGGGAGCG
    GCTTTGGATG CAGTTAAGGT GGAGAAGGAG AAACTGCAGA AGGAGATAAT GAGTGGGTTA
    TATCGTGATA TGAGGCGGTT GAGGAAGGAG AGAGATTTGT TAATGAAACG AGCTGATAAA
    ATTGTAGATG AGGCCCTAAG TTTGAAGAAA CAGAGTGAAA AACTGTTGAG AAAAGGTGCT
    AGGGAGAAAA TGGAGAAACT GGAAGAGAGT GTGGATATTA TGGAGAGCGA GTACAATAAG
    ATATGGGAAA GAATAGATGA GATTGATGAT ATCATTTTAA AGAAAGAGAC AACAACATTG
    AGTTTTGGGG TTAGGGAGCT TATTTTCATT GAAAGAGAGT GTGTAGAGTT GGTTAAGAGC
    TTCAACCGAG AATTGAATCA AAAAAGTTTT GAAAGTGTTC CTGAAAGCTC CATTACAAAA
    CTCTCGAGGT CTGAGATTAA GCAGGAATTA GTGAACGCTC AAAGAAAGCA CTTGGAACAA
    ATGATACTGC CCAATGTTTT GGAACTAGAG GAGGTTGATC CCTTTTTCGA TCGTGACTCA
    GTGGATTTTT CTCTACGCAT CAAAAAACGC CTTGAGGAAT CAAAAAAGCT GCAGAGAGAT
    CTTCAGAATC GTATCAGAAA ACGTATGAAA AAGTTTGGTG AAGAAAAGCT TTTTGTTCAA
    AAGACTCCAG AAGGTGAAGC TGTCAAGGGA TTTCCTGAAG CAGAGGTGAA ATGGATGTTT
    GGAGAGAAAG AGGTGGTTGT TCCGAAAGCT ATCCAGCTCC ATCTACGCCA TGGTTGGAAA
    AAGTGGCAGG AAGAAGCAAA AGCCGATTTA AAACAGAAAC TTTTGGAAGA TGTTGATTTT
    GGCAAACAAT ATATTGCGCA GAGACAGGAA CAAGTTCTCC TGGATCGAGA TAGAGTTGTT
    TCGAAGACTT GGTATAACGA AGACAAAAGT AGGTGGGAGA TGGACCCTAT GGCTGTTCCC
    TACGCAGTTT CCAGGAAGCT GATTGACAGT GCCAGGATTA GGCATGATTA TGCTGTAATG
    TATGTTGCAT TAAAAGGAGA TGACAAGGAG TTTTACGTTG ATATCAAGGA ATATGAGATG
    CTATTTGAGA AATTTGGAGG GTTTGATGCT CTGTACCTCA AAATGCTTGC TTGTGGAATC
    CCAACTTCTG TTCATCTGAT GTGGATACCC ATGTCAGAGC TAAGTCTTCA ACAACAGTTT
    CTGTTGGTTA CAAGGGTGGT TTCTCGAGTC TTTAACGCAT TGAGGAAGAC TCAAGTTGTA
    TCAAATGCGA AGGATACAGT ATTAGAGAAA ATAAGGAATA TAAACGATGA CATTATGATG
    GCGGTTGTGT TTCCAGTTAT CGAATTTATC ATTCCTTACC AGTTAAGGCT GCGTCTGGGA
    ATGGCTTGGC CAGAAGAAAT AGAACAGACT GTTGGCTCAA CATGGTACTT ACAATGGCAG
    TCTGAAGCAG AAATGAATTT CAAGTCCCGC AATACAGAAG ATTTTCAGTG GTTCCTCTGG
    TTTCTAATTC GAAGTTCTAT ATACGGGTTT GTTTTGTATC ACGTTTTCCG CTTTCTAAAA
    AGAAAGGTTC CAAGACTTCT TGGCTATGGA CCCTTCCGCC GAGATCCCAA CGTACGGAAA
    TTCTGGAGAG TGAAATCATA TTTCACTTAT AGAAAAAGGA GAATCAAACA AAAGAGAAAG
    GCTGGAATCG ACCCCATAAA AACTGCATTC GACAGAATGA AGAGAGTGAA AAATCCACCT
    ATCCCATTAA AGAACTTTGC TAGTATTGAG TCTATGAGAG AAGAAATCAA CGAGGTTGTG
    GCATTTTTAC AGAATCCAAA GGCATTTCAG GAGATGGGAG CCCGCGCACC TCGGGGCGTT
    CTTATTGTGG GTGAGAGAGG AACGGGAAAG ACGTCACTGG CGCTCGCTAT AGCAGCCGAA
    GCAAGAGTGC CTGTTGTCAA TGTTGAAGCT CAAGAGTTGG AAGCTGGGCT ATGGGTTGGT
    CAAAGTGCAG CGAATGTCAG GGAACTCTTT CAAACTGCAA GAGACTTGGC ACCTGTAATC
    ATTTTTGTGG AGGATTTTGA CCTCTTTGCT GGTGTACGCG GGAAGTTTGT ACATACAAAA
    CAGCAAGATC ATGAATCGTT CATTAATCAG CTTCTTGTTG AACTTGATGG CTTTGAGAAA
    CAGGATGGCG TAGTTTTGAT GGCGACGACA CGAAATCATA AGCAAATTGA TGAGGCGCTA
    AGAAGGCCAG GTCGTATGGA TAGAGTATTC CATCTTCAAA GTCCAACTGA AATGGAAAGG
    GAGAGGATTT TACACAACGC TGCAGAAGAA ACCATGGACC GAGAACTCGT TGATCTAGTG
    GATTGGCGTA AGGTTTCAGA GAAGACAACT TTATTAAGAC CGATAGAACT GAAACTTGTT
    CCAATGGCTC TCGAAAGTAG TGCCTTCAGA AGCAAATTTC TGGACACTGA CGAACTTCTG
    AGTTACGTTA GCTGGTTTGC GACGTTTAGC CATATAGTGC CCCCATGGCT GCGAAAAACT
    AAAGTTGCAA AGACAATGGG CAAAATGCTT GTGAATCATC TTGGACTTAA CTTAACCAAA
    GATGATTTGG AGAATGTGGT TGATCTGATG GAACCATATG GACAAATAAG TAATGGAATA
    GAACTTTTGA ATCCTACTGT TGATTGGACG AGGGAGACAA AGTTCCCACA TGCTGTCTGG
    GCGGCTGGTC GTGCTCTCAT CACACTTCTA ATACCAAACT TTGATGTTGT GGAAAATCTT
    TGGCTTGAGC CTAGTTCCTG GGAAGGAATT GGGTGTACAA AAATCACCAA GGTCACAAGT
    GGAGGCTCCG CGATTGGGAA CACGGAATCA AGATCATATC TTGAAAAGAA GCTCGTTTTC
    TGCTTTGGAT CTCACATTGC ATCCCAAATG CTTCTTCCTC CCGGAGATGA AAATTTTCTT
    TCTTCTTCAG AAATAACTAA AGCGCAGGAG ATTGCAACAC GTATGGTGCT TCAATATGGC
    TGGGGACCCG ATGACAGTCC TGCAGTATAC TATGCTACTA ACGCGGTTTC AGCTTTGAGT
    ATGGGGAACA ATCACGAATA TGAAATGGCG GGTAAAGTTG AAAAGATTTA TGACTTAGCG
    TACGAAAAGG CAAAGGGAAT GCTCCTTAAA AATCGACGTG TCCTTGAGAA AATCACAGAG
    GAGCTACTCG AATTTGAGAT TTTAACTCAT AAGGATTTGG AAAGAATTGT TCATGAGAAT
    GGTGGGATTC GGGAGAAGGA ACCTTTCTTT CTATCAGGAA CCAATTACAA TGAGGCATTG
    TCAAGGAGTT TTCTTGATGT TGGAGATCCG CCAGAAACTG CACTTCTTAG TGCACCAACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_187084.6
    CDS310..4272
    Translation

    Target ORF information:

    RefSeq Version NM_111305.7
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana FtsH extracellular protease family (emb2458), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_111305.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATTTCA TTTCCGCTTC TTCCTTATCT TCACCCTTTT CCACACAGTT GTCTCCGATT 
    TATTTGAGCT CAGGTATAGT ATCGTTAAAG CCTAGACATC GAGTCAAAAA TCGAAACTTT
    GGGTCTAGAG AATCCAATAA TAAATCTAGG AAAATTGTGC CCATTAGAGG CTGCTTTGGT
    TTTTCCGGGA GTTTTTTGAG GTCGAAACAG TCTGATTACG GTAGTGAAGC TGTTTCGGAA
    TCATTGAGAT TATGTGGAGA GGGAAATGAG CTTGTGCTAT CTTCCGAGTA TAATAGCGCA
    AAGACGAGAG AGAGCGTAAT ACAGTTTGTT ACCAAGCCTC TAGTTTACGC CTTGTTTTGT
    ATTGCAATTG GACTTTCTCC GATTCGCTCT TTTCAAGCTC CAGCTTTAGC TGTGCCTTTC
    GTCAGTGATG TTATTTGGAA GAAAAAGAAA GAGAGAGTGA GAGAGAAAGA GGTGGTTTTG
    AAAGCAGTTG ATCATGAGTT CTCGGATTAT ACTCGGAGAT TGTTGGAGAC AGTCTCGGTT
    TTACTGAAGA CTATTGAGAT AGTTAGAAAG GAAAATGGAG AGGTTGCTGA AGTGGGAGCG
    GCTTTGGATG CAGTTAAGGT GGAGAAGGAG AAACTGCAGA AGGAGATAAT GAGTGGGTTA
    TATCGTGATA TGAGGCGGTT GAGGAAGGAG AGAGATTTGT TAATGAAACG AGCTGATAAA
    ATTGTAGATG AGGCCCTAAG TTTGAAGAAA CAGAGTGAAA AACTGTTGAG AAAAGGTGCT
    AGGGAGAAAA TGGAGAAACT GGAAGAGAGT GTGGATATTA TGGAGAGCGA GTACAATAAG
    ATATGGGAAA GAATAGATGA GATTGATGAT ATCATTTTAA AGAAAGAGAC AACAACATTG
    AGTTTTGGGG TTAGGGAGCT TATTTTCATT GAAAGAGAGT GTGTAGAGTT GGTTAAGAGC
    TTCAACCGAG AATTGAATCA AAAAAGTTTT GAAAGTGTTC CTGAAAGCTC CATTACAAAA
    CTCTCGAGGT CTGAGATTAA GCAGGAATTA GTGAACGCTC AAAGAAAGCA CTTGGAACAA
    ATGATACTGC CCAATGTTTT GGAACTAGAG GAGGTTGATC CCTTTTTCGA TCGTGACTCA
    GTGGATTTTT CTCTACGCAT CAAAAAACGC CTTGAGGAAT CAAAAAAGCT GCAGAGAGAT
    CTTCAGAATC GTATCAGAAA ACGTATGAAA AAGTTTGGTG AAGAAAAGCT TTTTGTTCAA
    AAGACTCCAG AAGGTGAAGC TGTCAAGGGA TTTCCTGAAG CAGAGGTGAA ATGGATGTTT
    GGAGAGAAAG AGGTGGTTGT TCCGAAAGCT ATCCAGCTCC ATCTACGCCA TGGTTGGAAA
    AAGTGGCAGG AAGAAGCAAA AGCCGATTTA AAACAGAAAC TTTTGGAAGA TGTTGATTTT
    GGCAAACAAT ATATTGCGCA GAGACAGGAA CAAGTTCTCC TGGATCGAGA TAGAGTTGTT
    TCGAAGACTT GGTATAACGA AGACAAAAGT AGGTGGGAGA TGGACCCTAT GGCTGTTCCC
    TACGCAGTTT CCAGGAAGCT GATTGACAGT GCCAGGATTA GGCATGATTA TGCTGTAATG
    TATGTTGCAT TAAAAGGAGA TGACAAGGAG TTTTACGTTG ATATCAAGGA ATATGAGATG
    CTATTTGAGA AATTTGGAGG GTTTGATGCT CTGTACCTCA AAATGCTTGC TTGTGGAATC
    CCAACTTCTG TTCATCTGAT GTGGATACCC ATGTCAGAGC TAAGTCTTCA ACAACAGTTT
    CTGTTGGTTA CAAGGGTGGT TTCTCGAGTC TTTAACGCAT TGAGGAAGAC TCAAGTTGTA
    TCAAATGCGA AGGATACAGT ATTAGAGAAA ATAAGGAATA TAAACGATGA CATTATGATG
    GCGGTTGTGT TTCCAGTTAT CGAATTTATC ATTCCTTACC AGTTAAGGCT GCGTCTGGGA
    ATGGCTTGGC CAGAAGAAAT AGAACAGACT GTTGGCTCAA CATGGTACTT ACAATGGCAG
    TCTGAAGCAG AAATGAATTT CAAGTCCCGC AATACAGAAG ATTTTCAGTG GTTCCTCTGG
    TTTCTAATTC GAAGTTCTAT ATACGGGTTT GTTTTGTATC ACGTTTTCCG CTTTCTAAAA
    AGAAAGGTTC CAAGACTTCT TGGCTATGGA CCCTTCCGCC GAGATCCCAA CGTACGGAAA
    TTCTGGAGAG TGAAATCATA TTTCACTTAT AGAAAAAGGA GAATCAAACA AAAGAGAAAG
    GCTGGAATCG ACCCCATAAA AACTGCATTC GACAGAATGA AGAGAGTGAA AAATCCACCT
    ATCCCATTAA AGAACTTTGC TAGTATTGAG TCTATGAGAG AAGAAATCAA CGAGGTTGTG
    GCATTTTTAC AGAATCCAAA GGCATTTCAG GAGATGGGAG CCCGCGCACC TCGGGGCGTT
    CTTATTGTGG GTGAGAGAGG AACGGGAAAG ACGTCACTGG CGCTCGCTAT AGCAGCCGAA
    GCAAGAGTGC CTGTTGTCAA TGTTGAAGCT CAAGAGTTGG AAGCTGGGCT ATGGGTTGGT
    CAAAGTGCAG CGAATGTCAG GGAACTCTTT CAAACTGCAA GAGACTTGGC ACCTGTAATC
    ATTTTTGTGG AGGATTTTGA CCTCTTTGCT GGTGTACGCG GGAAGTTTGT ACATACAAAA
    CAGCAAGATC ATGAATCGTT CATTAATCAG CTTCTTGTTG AACTTGATGG CTTTGAGAAA
    CAGGATGGCG TAGTTTTGAT GGCGACGACA CGAAATCATA AGCAAATTGA TGAGGCGCTA
    AGAAGGCCAG GTCGTATGGA TAGAGTATTC CATCTTCAAA GTCCAACTGA AATGGAAAGG
    GAGAGGATTT TACACAACGC TGCAGAAGAA ACCATGGACC GAGAACTCGT TGATCTAGTG
    GATTGGCGTA AGGTTTCAGA GAAGACAACT TTATTAAGAC CGATAGAACT GAAACTTGTT
    CCAATGGCTC TCGAAAGTAG TGCCTTCAGA AGCAAATTTC TGGACACTGA CGAACTTCTG
    AGTTACGTTA GCTGGTTTGC GACGTTTAGC CATATAGTGC CCCCATGGCT GCGAAAAACT
    AAAGTTGCAA AGACAATGGG CAAAATGCTT GTGAATCATC TTGGACTTAA CTTAACCAAA
    GATGATTTGG AGAATGTGGT TGATCTGATG GAACCATATG GACAAATAAG TAATGGAATA
    GAACTTTTGA ATCCTACTGT TGATTGGACG AGGGAGACAA AGTTCCCACA TGCTGTCTGG
    GCGGCTGGTC GTGCTCTCAT CACACTTCTA ATACCAAACT TTGATGTTGT GGAAAATCTT
    TGGCTTGAGC CTAGTTCCTG GGAAGGAATT GGGTGTACAA AAATCACCAA GGTCACAAGT
    GGAGGCTCCG CGATTGGGAA CACGGAATCA AGATCATATC TTGAAAAGAA GCTCGTTTTC
    TGCTTTGGAT CTCACATTGC ATCCCAAATG CTTCTTCCTC CCGGAGATGA AAATTTTCTT
    TCTTCTTCAG AAATAACTAA AGCGCAGGAG ATTGCAACAC GTATGGTGCT TCAATATGGC
    TGGGGACCCG ATGACAGTCC TGCAGTATAC TATGCTACTA ACGCGGTTTC AGCTTTGAGT
    ATGGGGAACA ATCACGAATA TGAAATGGCG GGTAAAGTTG AAAAGATTTA TGACTTAGCG
    TACGAAAAGG CAAAGGGAAT GCTCCTTAAA AATCGACGTG TCCTTGAGAA AATCACAGAG
    GAGCTACTCG AATTTGAGAT TTTAACTCAT AAGGATTTGG AAAGAATTGT TCATGAGAAT
    GGTGGGATTC GGGAGAAGGA ACCTTTCTTT CTATCAGGAA CCAATTACAA TGAGGCATTG
    TCAAGGAGTT TTCTTGATGT TGGAGATCCG CCAGAAACTG CACTTCTTAG TGCACCAACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
    Nature408(6814)820-2(2000 Dec)
    Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,