SPA1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPA1
Entrez Gene ID	819242
Full Name	SPA (suppressor of phyA-105) protein family
Synonyms	AT2G46350,F11C10.3,SUPPRESSOR OF PHYA-105 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001337186.1	NP_001325255.1	SPA (suppressor of phyA-105) protein family
NM_130197.5	NP_182157.2	SPA (suppressor of phyA-105) protein family

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	SPA1	NP_182157.2
Oryza sativa (rice)	Os05g0571000	NP_001056370.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPA proteins have a dual role: (1) they are required for light-responsiveness of COP1 subcellular localization, and (2) they promote COP1 activity in darkness in a fashion that is independent of the nuclear import/nuclear retention of COP1.
Title: SPA Proteins Affect the Subcellular Localization of COP1 in the COP1/SPA Ubiquitin Ligase Complex during Photomorphogenesis.

The N-terminal kinase-like domain is primarily responsible for the respective light-responsiveness of SPA1 and SPA2.
Title: The functional divergence between SPA1 and SPA2 in Arabidopsis photomorphogenesis maps primarily to the respective N-terminal kinase-like domain.

CUL4(COP1-SPA) E3 ubiquitin ligase is necessary for the light-induced degradation of PIF1 in Arabidopsis.
Title: CUL4 forms an E3 ligase with COP1 and SPA to promote light-induced degradation of PIF1.

Interaction of phyB with SPA1 promotes COP1-SPA1 dissociation and photomorphogenic development.
Title: Red-light-dependent interaction of phyB with SPA1 promotes COP1-SPA1 dissociation and photomorphogenic development in Arabidopsis.

It was shown show that mutations in the key repressor of light signaling, the COP1/SPA complex, cause a strong hyperaccumulation of anthocyanins not only under normal light but also under high light conditions.
Title: COP1/SPA ubiquitin ligase complexes repress anthocyanin accumulation under low light and high light conditions.

The following SPA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb09936	NM_001337186.1 Latest version!	Arabidopsis thaliana SPA (suppressor of phyA-105) protein family (SPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb09936	NM_130197.5 Latest version!	Arabidopsis thaliana SPA (suppressor of phyA-105) protein family (SPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPA1 ( NM_001337186.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001325255.1 Arabidopsis thaliana SPA (suppressor of phyA-105) protein family (SPA1), mRNA.

Price & Availability↑

CloneID	OAb09936
Clone ID Related Accession (Same CDS sequence)	NM_001337186.1 , NM_130197.5
Accession Version	NM_001337186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	SPA (suppressor of phyA-105) protein family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGCCTGTTA TGGAAAGAGT AGCTGAAGAA ACTGTGGCGA CTAACAACAT ACAATTGAAA 
GCACGAGTGG ATGATGTTCC TTGTAATAAG CTTGATGCTC GTCATAACGA TATGGTGATT 
CAATCAGAAA CTGCAAACAG TGATTGTCCT GGAAGCTCAG CTCACAGGAA TGTTGATTTG 
ACTAAGCCGC CGCCTCCTGA GGAAGCCGCA GGAGCTAAGC TTTCTGTTGA AGAGTTGACA 
TTGGGGAACT ACAGGATTGT TCAAGGGAGT AATAATACTA ATGTGGATAG TCCTAGGGCT 
GGCAAGTTTG AGCATCTTTA TCGTTTGGCT AGAGGGTCTG CTTTTAGGGC AGGAGATGGG 
GATCTTGATT CTCAACCTCG TGATATGGAT CAAATGTTGT CCAGAATCAG ACAACAGCTT 
GCTGGAGCTC CTTCTGAGAG GCAAAACTTG AAACCTTTTA TGTCTAGGAG GAGCGATCAG 
AATCTTGAAG CTTTTTCTGA ACGTCTAAGA GCAGCTGGTG AGAATAGTAT TATGAATGCT 
CCTGCATTGA TCAGTGAAGG TGTCCAAATG AAAACGCCGG TGAGTTCTTC GAATTTTTCT 
CAGTTGTTAC TTAAAAGAGC TATGAAAGGG AAAGGGGTAG TAGGAAAGAA CCAGGAAACT 
CCTCCTGAAT TTGTCTCTGA TCAAGATTTA GGAAGCAAGG AAAAAAAGTT GGATATTAGC 
AAGTCGCCTA CTCCTCACGA TGTGTTACCT CTAAAATCAA GTCCCAAAGG AAATGGTATG 
GTATCACATG GTGATGGGAA TCATAGTAAA TCTTCTATTG GAATCAGTTT GAGGGAGTTT 
CTTAGATCAA GTTATGCTAA AAGAGAAAAA AGACATGGCC TTTGTCTATT TAGGCAGCTG 
GTGGAACTGG TTGACTCAGC CCATTCAAAA AGATTATTCT TGCTAGACTT GAGACCATCC 
CTTTTCACTT TAGTTCCATC AAAGAAACTT AGATATATTG GTAATTTTGG AAAGAACGAT 
CTAGAATCAG ATGTTGACGA AGATTTGAAT AGGAGGAGGC CTGTTGTTGA GGAATCATCT 
AGTGGAGGTA GGGATTCGAA GAAAAGAAAA ATGGACTTGC ATTTGAATTC TCCAGGGAAT 
CAACTTCAGG CCACTTCAAC CGGGCGACCT TTCAAAAGGA AAAGCCCTGT GATTGATTTA 
AACATGGTTG ATGCACGTAA TCCAGACAGT TGCGAGCTTC AACAGCAAGA CTACATAAAA 
AATTTAAGCG TGTCTTCAGT GTCGAGAAAA CAGTCTATGT CCACATGGCT AGAAGAGCAA 
TGGTATACTT GTCCAGAGGA GATAAATGGA GAAGATATTG GAGAAAAATC AAATATTTAT 
GCCCTTGGTG TTCTTCTGTT TGAGTTGCTA TGCCATTGCG AGTCAGGCGA GATGCATGCT 
GCAATGATGG CAGATTTACG CCATCGGATT CTCCCACCAA CATTTCTCTC GAAATACCCG 
AAGGAAGCTG GATTTTGTCT TTGGCTTCTA CATCCTGAAC CCTCCTCCCG ACCATCAGCT 
AGAGATATAC TAAAGTCAGA GTTGATATGC GAGGATGATT CAGTTAAATC GACTGCTGCT 
GCTGAGGAGA TATCTGAGCT GTTACTTCAT TTTTTGTCTT CATTAGAAGT GCAGAAGAAG 
AAAAAAGCAT CTAAACTTTT GCAAGATATC CAGACCTTAG AGGATGATAT TAAGGAGGCC 
GAGCGAAGAT ATTCTTCAAA TGTATCTCTG GTGAGATCTC ATGGAGCTAT TGAAAAAAGG 
GTGCAATCAT CTCCCCTAGA CGAGCACTGT ACAACTTCCA GTGCCTTGTT TGTACCAACT 
GCTAATACAG ACAGGCTGAT GAGTAATATC CGTCAACTTG AAGATGCATA TTTTTTCATG 
CGATCACAAA TCAACTTATC GAGTTCTGCT GCTACTGCCC GTTCTGACAA AACACTAAAG 
GACAGGGACA GATGCTCTGA AAACCAAAAT GAAAATCAGG ATATGAGTAC CAAAGGAAAA 
TCTTCAGATC AACTCGAAGT GTTTTTCGAG GGGTTGTGCA AATTTGCTCG GTATAGCAAG 
TTCGAAACCT GTGGGACAAT AAGAAGTGGG GACCTTTTAA ACTCTGCAAG TGTGGTCTGC 
TCGTTGAGTT TTGACCCTGA TGAAGAACAC ATAGCAGCAG CTGGGATATC AAAGAAGATC 
AAGATTTTTG ACTTCAATGC ATTTATGAAT GAATCTGTCG GTGTTCATTA TCCACTAGTA 
GAGATGGTCA ACAAATCTAA ATTAAGCTGT GTTTGCTGGA ATAGCTACAT CAAAAACTAC 
TTGGCCTCGA CTGACTACGA TGGTGTAGTT CAGATATGGG ATGCCGGCAC TGGACAAGGA 
TTTTCGCAGT ACACAGAACA CCAGAAGAGA GCCTGGTCAG TTGATTTTTC TCCATCTGAC 
CCGACAAAAT TTGTTAGTGG AAGTGATGAC TGCTCGGTGA AGCTTTGGAG CATCAATGAG 
AAACGTTCAT TAGGCACAAT CTGGAGTCCA GCAAATGTGT GTTGCGTGCA ATTCTCTTCT 
TATTCCAATC ACTTATTGGC ATTTGGGTCA GCTGATTACA AGGTCTATTG CTATGACCTT 
CGGTATGTCA AAACACCTTG GTGCACATTG GCTGGTCATG AGAAAGCGGT GAGTTATGTT 
AAATTTATGG ATTCAGAAAC CATTGTTTCT GCTTCCACTG ATAACTCTCT CAAGCTTTGG 
AATCTTAACA AGACAAATTC CTCTGGTTTA TCTCCTGGTG CTTGTTCATT GACATATAAA 
GGACATACAA ATCAGAAGAA TTTCGTTGGA TTGTCGGTTT TGGACGGATA CATAGCTTGT 
GGTTCAGAGA CCAATGAGGT ATATAGTTAC TATAAATCTT TACCGATGCC AATGACTTCA 
TACAAGTTTG GATCCGTCGA TCCCATTTCT GGAAACGAAT ACTTTGATGA CAATGGACAG 
TTTGTGTCGA GCGTCTGTTG GAGGAAAAAA TCGAATATGC TTGTAGCTGC AAACTCTACT 
GGAAACATGA AGCTACTAAA ACTTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001325255.1
CDS	605..3694
Translation	MPVMERVAEETVATNNIQLKARVDDVPCNKLDARHNDMVIQSETANSDCPGSSAHRNVDLTKPPPPEEAAGAKLSVEELTLGNYRIVQGSNNTNVDSPRAGKFEHLYRLARGSAFRAGDGDLDSQPRDMDQMLSRIRQQLAGAPSERQNLKPFMSRRSDQNLEAFSERLRAAGENSIMNAPALISEGVQMKTPVSSSNFSQLLLKRAMKGKGVVGKNQETPPEFVSDQDLGSKEKKLDISKSPTPHDVLPLKSSPKGNGMVSHGDGNHSKSSIGISLREFLRSSYAKREKRHGLCLFRQLVELVDSAHSKRLFLLDLRPSLFTLVPSKKLRYIGNFGKNDLESDVDEDLNRRRPVVEESSSGGRDSKKRKMDLHLNSPGNQLQATSTGRPFKRKSPVIDLNMVDARNPDSCELQQQDYIKNLSVSSVSRKQSMSTWLEEQWYTCPEEINGEDIGEKSNIYALGVLLFELLCHCESGEMHAAMMADLRHRILPPTFLSKYPKEAGFCLWLLHPEPSSRPSARDILKSELICEDDSVKSTAAAEEISELLLHFLSSLEVQKKKKASKLLQDIQTLEDDIKEAERRYSSNVSLVRSHGAIEKRVQSSPLDEHCTTSSALFVPTANTDRLMSNIRQLEDAYFFMRSQINLSSSAATARSDKTLKDRDRCSENQNENQDMSTKGKSSDQLEVFFEGLCKFARYSKFETCGTIRSGDLLNSASVVCSLSFDPDEEHIAAAGISKKIKIFDFNAFMNESVGVHYPLVEMVNKSKLSCVCWNSYIKNYLASTDYDGVVQIWDAGTGQGFSQYTEHQKRAWSVDFSPSDPTKFVSGSDDCSVKLWSINEKRSLGTIWSPANVCCVQFSSYSNHLLAFGSADYKVYCYDLRYVKTPWCTLAGHEKAVSYVKFMDSETIVSASTDNSLKLWNLNKTNSSGLSPGACSLTYKGHTNQKNFVGLSVLDGYIACGSETNEVYSYYKSLPMPMTSYKFGSVDPISGNEYFDDNGQFVSSVCWRKKSNMLVAANSTGNMKLLKLV

Target ORF information:

RefSeq Version	NM_001337186.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana SPA (suppressor of phyA-105) protein family (SPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001337186.1

ORF Insert Sequence:

ATGCCTGTTA TGGAAAGAGT AGCTGAAGAA ACTGTGGCGA CTAACAACAT ACAATTGAAA 
GCACGAGTGG ATGATGTTCC TTGTAATAAG CTTGATGCTC GTCATAACGA TATGGTGATT 
CAATCAGAAA CTGCAAACAG TGATTGTCCT GGAAGCTCAG CTCACAGGAA TGTTGATTTG 
ACTAAGCCGC CGCCTCCTGA GGAAGCCGCA GGAGCTAAGC TTTCTGTTGA AGAGTTGACA 
TTGGGGAACT ACAGGATTGT TCAAGGGAGT AATAATACTA ATGTGGATAG TCCTAGGGCT 
GGCAAGTTTG AGCATCTTTA TCGTTTGGCT AGAGGGTCTG CTTTTAGGGC AGGAGATGGG 
GATCTTGATT CTCAACCTCG TGATATGGAT CAAATGTTGT CCAGAATCAG ACAACAGCTT 
GCTGGAGCTC CTTCTGAGAG GCAAAACTTG AAACCTTTTA TGTCTAGGAG GAGCGATCAG 
AATCTTGAAG CTTTTTCTGA ACGTCTAAGA GCAGCTGGTG AGAATAGTAT TATGAATGCT 
CCTGCATTGA TCAGTGAAGG TGTCCAAATG AAAACGCCGG TGAGTTCTTC GAATTTTTCT 
CAGTTGTTAC TTAAAAGAGC TATGAAAGGG AAAGGGGTAG TAGGAAAGAA CCAGGAAACT 
CCTCCTGAAT TTGTCTCTGA TCAAGATTTA GGAAGCAAGG AAAAAAAGTT GGATATTAGC 
AAGTCGCCTA CTCCTCACGA TGTGTTACCT CTAAAATCAA GTCCCAAAGG AAATGGTATG 
GTATCACATG GTGATGGGAA TCATAGTAAA TCTTCTATTG GAATCAGTTT GAGGGAGTTT 
CTTAGATCAA GTTATGCTAA AAGAGAAAAA AGACATGGCC TTTGTCTATT TAGGCAGCTG 
GTGGAACTGG TTGACTCAGC CCATTCAAAA AGATTATTCT TGCTAGACTT GAGACCATCC 
CTTTTCACTT TAGTTCCATC AAAGAAACTT AGATATATTG GTAATTTTGG AAAGAACGAT 
CTAGAATCAG ATGTTGACGA AGATTTGAAT AGGAGGAGGC CTGTTGTTGA GGAATCATCT 
AGTGGAGGTA GGGATTCGAA GAAAAGAAAA ATGGACTTGC ATTTGAATTC TCCAGGGAAT 
CAACTTCAGG CCACTTCAAC CGGGCGACCT TTCAAAAGGA AAAGCCCTGT GATTGATTTA 
AACATGGTTG ATGCACGTAA TCCAGACAGT TGCGAGCTTC AACAGCAAGA CTACATAAAA 
AATTTAAGCG TGTCTTCAGT GTCGAGAAAA CAGTCTATGT CCACATGGCT AGAAGAGCAA 
TGGTATACTT GTCCAGAGGA GATAAATGGA GAAGATATTG GAGAAAAATC AAATATTTAT 
GCCCTTGGTG TTCTTCTGTT TGAGTTGCTA TGCCATTGCG AGTCAGGCGA GATGCATGCT 
GCAATGATGG CAGATTTACG CCATCGGATT CTCCCACCAA CATTTCTCTC GAAATACCCG 
AAGGAAGCTG GATTTTGTCT TTGGCTTCTA CATCCTGAAC CCTCCTCCCG ACCATCAGCT 
AGAGATATAC TAAAGTCAGA GTTGATATGC GAGGATGATT CAGTTAAATC GACTGCTGCT 
GCTGAGGAGA TATCTGAGCT GTTACTTCAT TTTTTGTCTT CATTAGAAGT GCAGAAGAAG 
AAAAAAGCAT CTAAACTTTT GCAAGATATC CAGACCTTAG AGGATGATAT TAAGGAGGCC 
GAGCGAAGAT ATTCTTCAAA TGTATCTCTG GTGAGATCTC ATGGAGCTAT TGAAAAAAGG 
GTGCAATCAT CTCCCCTAGA CGAGCACTGT ACAACTTCCA GTGCCTTGTT TGTACCAACT 
GCTAATACAG ACAGGCTGAT GAGTAATATC CGTCAACTTG AAGATGCATA TTTTTTCATG 
CGATCACAAA TCAACTTATC GAGTTCTGCT GCTACTGCCC GTTCTGACAA AACACTAAAG 
GACAGGGACA GATGCTCTGA AAACCAAAAT GAAAATCAGG ATATGAGTAC CAAAGGAAAA 
TCTTCAGATC AACTCGAAGT GTTTTTCGAG GGGTTGTGCA AATTTGCTCG GTATAGCAAG 
TTCGAAACCT GTGGGACAAT AAGAAGTGGG GACCTTTTAA ACTCTGCAAG TGTGGTCTGC 
TCGTTGAGTT TTGACCCTGA TGAAGAACAC ATAGCAGCAG CTGGGATATC AAAGAAGATC 
AAGATTTTTG ACTTCAATGC ATTTATGAAT GAATCTGTCG GTGTTCATTA TCCACTAGTA 
GAGATGGTCA ACAAATCTAA ATTAAGCTGT GTTTGCTGGA ATAGCTACAT CAAAAACTAC 
TTGGCCTCGA CTGACTACGA TGGTGTAGTT CAGATATGGG ATGCCGGCAC TGGACAAGGA 
TTTTCGCAGT ACACAGAACA CCAGAAGAGA GCCTGGTCAG TTGATTTTTC TCCATCTGAC 
CCGACAAAAT TTGTTAGTGG AAGTGATGAC TGCTCGGTGA AGCTTTGGAG CATCAATGAG 
AAACGTTCAT TAGGCACAAT CTGGAGTCCA GCAAATGTGT GTTGCGTGCA ATTCTCTTCT 
TATTCCAATC ACTTATTGGC ATTTGGGTCA GCTGATTACA AGGTCTATTG CTATGACCTT 
CGGTATGTCA AAACACCTTG GTGCACATTG GCTGGTCATG AGAAAGCGGT GAGTTATGTT 
AAATTTATGG ATTCAGAAAC CATTGTTTCT GCTTCCACTG ATAACTCTCT CAAGCTTTGG 
AATCTTAACA AGACAAATTC CTCTGGTTTA TCTCCTGGTG CTTGTTCATT GACATATAAA 
GGACATACAA ATCAGAAGAA TTTCGTTGGA TTGTCGGTTT TGGACGGATA CATAGCTTGT 
GGTTCAGAGA CCAATGAGGT ATATAGTTAC TATAAATCTT TACCGATGCC AATGACTTCA 
TACAAGTTTG GATCCGTCGA TCCCATTTCT GGAAACGAAT ACTTTGATGA CAATGGACAG 
TTTGTGTCGA GCGTCTGTTG GAGGAAAAAA TCGAATATGC TTGTAGCTGC AAACTCTACT 
GGAAACATGA AGCTACTAAA ACTTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPA1 ( NM_130197.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_182157.2 Arabidopsis thaliana SPA (suppressor of phyA-105) protein family (SPA1), mRNA.

Price & Availability↑

CloneID	OAb09936
Clone ID Related Accession (Same CDS sequence)	NM_001337186.1 , NM_130197.5
Accession Version	NM_130197.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	SPA (suppressor of phyA-105) protein family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_130197.4.

ATGCCTGTTA TGGAAAGAGT AGCTGAAGAA ACTGTGGCGA CTAACAACAT ACAATTGAAA 
GCACGAGTGG ATGATGTTCC TTGTAATAAG CTTGATGCTC GTCATAACGA TATGGTGATT 
CAATCAGAAA CTGCAAACAG TGATTGTCCT GGAAGCTCAG CTCACAGGAA TGTTGATTTG 
ACTAAGCCGC CGCCTCCTGA GGAAGCCGCA GGAGCTAAGC TTTCTGTTGA AGAGTTGACA 
TTGGGGAACT ACAGGATTGT TCAAGGGAGT AATAATACTA ATGTGGATAG TCCTAGGGCT 
GGCAAGTTTG AGCATCTTTA TCGTTTGGCT AGAGGGTCTG CTTTTAGGGC AGGAGATGGG 
GATCTTGATT CTCAACCTCG TGATATGGAT CAAATGTTGT CCAGAATCAG ACAACAGCTT 
GCTGGAGCTC CTTCTGAGAG GCAAAACTTG AAACCTTTTA TGTCTAGGAG GAGCGATCAG 
AATCTTGAAG CTTTTTCTGA ACGTCTAAGA GCAGCTGGTG AGAATAGTAT TATGAATGCT 
CCTGCATTGA TCAGTGAAGG TGTCCAAATG AAAACGCCGG TGAGTTCTTC GAATTTTTCT 
CAGTTGTTAC TTAAAAGAGC TATGAAAGGG AAAGGGGTAG TAGGAAAGAA CCAGGAAACT 
CCTCCTGAAT TTGTCTCTGA TCAAGATTTA GGAAGCAAGG AAAAAAAGTT GGATATTAGC 
AAGTCGCCTA CTCCTCACGA TGTGTTACCT CTAAAATCAA GTCCCAAAGG AAATGGTATG 
GTATCACATG GTGATGGGAA TCATAGTAAA TCTTCTATTG GAATCAGTTT GAGGGAGTTT 
CTTAGATCAA GTTATGCTAA AAGAGAAAAA AGACATGGCC TTTGTCTATT TAGGCAGCTG 
GTGGAACTGG TTGACTCAGC CCATTCAAAA AGATTATTCT TGCTAGACTT GAGACCATCC 
CTTTTCACTT TAGTTCCATC AAAGAAACTT AGATATATTG GTAATTTTGG AAAGAACGAT 
CTAGAATCAG ATGTTGACGA AGATTTGAAT AGGAGGAGGC CTGTTGTTGA GGAATCATCT 
AGTGGAGGTA GGGATTCGAA GAAAAGAAAA ATGGACTTGC ATTTGAATTC TCCAGGGAAT 
CAACTTCAGG CCACTTCAAC CGGGCGACCT TTCAAAAGGA AAAGCCCTGT GATTGATTTA 
AACATGGTTG ATGCACGTAA TCCAGACAGT TGCGAGCTTC AACAGCAAGA CTACATAAAA 
AATTTAAGCG TGTCTTCAGT GTCGAGAAAA CAGTCTATGT CCACATGGCT AGAAGAGCAA 
TGGTATACTT GTCCAGAGGA GATAAATGGA GAAGATATTG GAGAAAAATC AAATATTTAT 
GCCCTTGGTG TTCTTCTGTT TGAGTTGCTA TGCCATTGCG AGTCAGGCGA GATGCATGCT 
GCAATGATGG CAGATTTACG CCATCGGATT CTCCCACCAA CATTTCTCTC GAAATACCCG 
AAGGAAGCTG GATTTTGTCT TTGGCTTCTA CATCCTGAAC CCTCCTCCCG ACCATCAGCT 
AGAGATATAC TAAAGTCAGA GTTGATATGC GAGGATGATT CAGTTAAATC GACTGCTGCT 
GCTGAGGAGA TATCTGAGCT GTTACTTCAT TTTTTGTCTT CATTAGAAGT GCAGAAGAAG 
AAAAAAGCAT CTAAACTTTT GCAAGATATC CAGACCTTAG AGGATGATAT TAAGGAGGCC 
GAGCGAAGAT ATTCTTCAAA TGTATCTCTG GTGAGATCTC ATGGAGCTAT TGAAAAAAGG 
GTGCAATCAT CTCCCCTAGA CGAGCACTGT ACAACTTCCA GTGCCTTGTT TGTACCAACT 
GCTAATACAG ACAGGCTGAT GAGTAATATC CGTCAACTTG AAGATGCATA TTTTTTCATG 
CGATCACAAA TCAACTTATC GAGTTCTGCT GCTACTGCCC GTTCTGACAA AACACTAAAG 
GACAGGGACA GATGCTCTGA AAACCAAAAT GAAAATCAGG ATATGAGTAC CAAAGGAAAA 
TCTTCAGATC AACTCGAAGT GTTTTTCGAG GGGTTGTGCA AATTTGCTCG GTATAGCAAG 
TTCGAAACCT GTGGGACAAT AAGAAGTGGG GACCTTTTAA ACTCTGCAAG TGTGGTCTGC 
TCGTTGAGTT TTGACCCTGA TGAAGAACAC ATAGCAGCAG CTGGGATATC AAAGAAGATC 
AAGATTTTTG ACTTCAATGC ATTTATGAAT GAATCTGTCG GTGTTCATTA TCCACTAGTA 
GAGATGGTCA ACAAATCTAA ATTAAGCTGT GTTTGCTGGA ATAGCTACAT CAAAAACTAC 
TTGGCCTCGA CTGACTACGA TGGTGTAGTT CAGATATGGG ATGCCGGCAC TGGACAAGGA 
TTTTCGCAGT ACACAGAACA CCAGAAGAGA GCCTGGTCAG TTGATTTTTC TCCATCTGAC 
CCGACAAAAT TTGTTAGTGG AAGTGATGAC TGCTCGGTGA AGCTTTGGAG CATCAATGAG 
AAACGTTCAT TAGGCACAAT CTGGAGTCCA GCAAATGTGT GTTGCGTGCA ATTCTCTTCT 
TATTCCAATC ACTTATTGGC ATTTGGGTCA GCTGATTACA AGGTCTATTG CTATGACCTT 
CGGTATGTCA AAACACCTTG GTGCACATTG GCTGGTCATG AGAAAGCGGT GAGTTATGTT 
AAATTTATGG ATTCAGAAAC CATTGTTTCT GCTTCCACTG ATAACTCTCT CAAGCTTTGG 
AATCTTAACA AGACAAATTC CTCTGGTTTA TCTCCTGGTG CTTGTTCATT GACATATAAA 
GGACATACAA ATCAGAAGAA TTTCGTTGGA TTGTCGGTTT TGGACGGATA CATAGCTTGT 
GGTTCAGAGA CCAATGAGGT ATATAGTTAC TATAAATCTT TACCGATGCC AATGACTTCA 
TACAAGTTTG GATCCGTCGA TCCCATTTCT GGAAACGAAT ACTTTGATGA CAATGGACAG 
TTTGTGTCGA GCGTCTGTTG GAGGAAAAAA TCGAATATGC TTGTAGCTGC AAACTCTACT 
GGAAACATGA AGCTACTAAA ACTTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_182157.2
CDS	426..3515
Translation	MPVMERVAEETVATNNIQLKARVDDVPCNKLDARHNDMVIQSETANSDCPGSSAHRNVDLTKPPPPEEAAGAKLSVEELTLGNYRIVQGSNNTNVDSPRAGKFEHLYRLARGSAFRAGDGDLDSQPRDMDQMLSRIRQQLAGAPSERQNLKPFMSRRSDQNLEAFSERLRAAGENSIMNAPALISEGVQMKTPVSSSNFSQLLLKRAMKGKGVVGKNQETPPEFVSDQDLGSKEKKLDISKSPTPHDVLPLKSSPKGNGMVSHGDGNHSKSSIGISLREFLRSSYAKREKRHGLCLFRQLVELVDSAHSKRLFLLDLRPSLFTLVPSKKLRYIGNFGKNDLESDVDEDLNRRRPVVEESSSGGRDSKKRKMDLHLNSPGNQLQATSTGRPFKRKSPVIDLNMVDARNPDSCELQQQDYIKNLSVSSVSRKQSMSTWLEEQWYTCPEEINGEDIGEKSNIYALGVLLFELLCHCESGEMHAAMMADLRHRILPPTFLSKYPKEAGFCLWLLHPEPSSRPSARDILKSELICEDDSVKSTAAAEEISELLLHFLSSLEVQKKKKASKLLQDIQTLEDDIKEAERRYSSNVSLVRSHGAIEKRVQSSPLDEHCTTSSALFVPTANTDRLMSNIRQLEDAYFFMRSQINLSSSAATARSDKTLKDRDRCSENQNENQDMSTKGKSSDQLEVFFEGLCKFARYSKFETCGTIRSGDLLNSASVVCSLSFDPDEEHIAAAGISKKIKIFDFNAFMNESVGVHYPLVEMVNKSKLSCVCWNSYIKNYLASTDYDGVVQIWDAGTGQGFSQYTEHQKRAWSVDFSPSDPTKFVSGSDDCSVKLWSINEKRSLGTIWSPANVCCVQFSSYSNHLLAFGSADYKVYCYDLRYVKTPWCTLAGHEKAVSYVKFMDSETIVSASTDNSLKLWNLNKTNSSGLSPGACSLTYKGHTNQKNFVGLSVLDGYIACGSETNEVYSYYKSLPMPMTSYKFGSVDPISGNEYFDDNGQFVSSVCWRKKSNMLVAANSTGNMKLLKLV

Target ORF information:

RefSeq Version	NM_130197.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana SPA (suppressor of phyA-105) protein family (SPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130197.5

ORF Insert Sequence:

ATGCCTGTTA TGGAAAGAGT AGCTGAAGAA ACTGTGGCGA CTAACAACAT ACAATTGAAA 
GCACGAGTGG ATGATGTTCC TTGTAATAAG CTTGATGCTC GTCATAACGA TATGGTGATT 
CAATCAGAAA CTGCAAACAG TGATTGTCCT GGAAGCTCAG CTCACAGGAA TGTTGATTTG 
ACTAAGCCGC CGCCTCCTGA GGAAGCCGCA GGAGCTAAGC TTTCTGTTGA AGAGTTGACA 
TTGGGGAACT ACAGGATTGT TCAAGGGAGT AATAATACTA ATGTGGATAG TCCTAGGGCT 
GGCAAGTTTG AGCATCTTTA TCGTTTGGCT AGAGGGTCTG CTTTTAGGGC AGGAGATGGG 
GATCTTGATT CTCAACCTCG TGATATGGAT CAAATGTTGT CCAGAATCAG ACAACAGCTT 
GCTGGAGCTC CTTCTGAGAG GCAAAACTTG AAACCTTTTA TGTCTAGGAG GAGCGATCAG 
AATCTTGAAG CTTTTTCTGA ACGTCTAAGA GCAGCTGGTG AGAATAGTAT TATGAATGCT 
CCTGCATTGA TCAGTGAAGG TGTCCAAATG AAAACGCCGG TGAGTTCTTC GAATTTTTCT 
CAGTTGTTAC TTAAAAGAGC TATGAAAGGG AAAGGGGTAG TAGGAAAGAA CCAGGAAACT 
CCTCCTGAAT TTGTCTCTGA TCAAGATTTA GGAAGCAAGG AAAAAAAGTT GGATATTAGC 
AAGTCGCCTA CTCCTCACGA TGTGTTACCT CTAAAATCAA GTCCCAAAGG AAATGGTATG 
GTATCACATG GTGATGGGAA TCATAGTAAA TCTTCTATTG GAATCAGTTT GAGGGAGTTT 
CTTAGATCAA GTTATGCTAA AAGAGAAAAA AGACATGGCC TTTGTCTATT TAGGCAGCTG 
GTGGAACTGG TTGACTCAGC CCATTCAAAA AGATTATTCT TGCTAGACTT GAGACCATCC 
CTTTTCACTT TAGTTCCATC AAAGAAACTT AGATATATTG GTAATTTTGG AAAGAACGAT 
CTAGAATCAG ATGTTGACGA AGATTTGAAT AGGAGGAGGC CTGTTGTTGA GGAATCATCT 
AGTGGAGGTA GGGATTCGAA GAAAAGAAAA ATGGACTTGC ATTTGAATTC TCCAGGGAAT 
CAACTTCAGG CCACTTCAAC CGGGCGACCT TTCAAAAGGA AAAGCCCTGT GATTGATTTA 
AACATGGTTG ATGCACGTAA TCCAGACAGT TGCGAGCTTC AACAGCAAGA CTACATAAAA 
AATTTAAGCG TGTCTTCAGT GTCGAGAAAA CAGTCTATGT CCACATGGCT AGAAGAGCAA 
TGGTATACTT GTCCAGAGGA GATAAATGGA GAAGATATTG GAGAAAAATC AAATATTTAT 
GCCCTTGGTG TTCTTCTGTT TGAGTTGCTA TGCCATTGCG AGTCAGGCGA GATGCATGCT 
GCAATGATGG CAGATTTACG CCATCGGATT CTCCCACCAA CATTTCTCTC GAAATACCCG 
AAGGAAGCTG GATTTTGTCT TTGGCTTCTA CATCCTGAAC CCTCCTCCCG ACCATCAGCT 
AGAGATATAC TAAAGTCAGA GTTGATATGC GAGGATGATT CAGTTAAATC GACTGCTGCT 
GCTGAGGAGA TATCTGAGCT GTTACTTCAT TTTTTGTCTT CATTAGAAGT GCAGAAGAAG 
AAAAAAGCAT CTAAACTTTT GCAAGATATC CAGACCTTAG AGGATGATAT TAAGGAGGCC 
GAGCGAAGAT ATTCTTCAAA TGTATCTCTG GTGAGATCTC ATGGAGCTAT TGAAAAAAGG 
GTGCAATCAT CTCCCCTAGA CGAGCACTGT ACAACTTCCA GTGCCTTGTT TGTACCAACT 
GCTAATACAG ACAGGCTGAT GAGTAATATC CGTCAACTTG AAGATGCATA TTTTTTCATG 
CGATCACAAA TCAACTTATC GAGTTCTGCT GCTACTGCCC GTTCTGACAA AACACTAAAG 
GACAGGGACA GATGCTCTGA AAACCAAAAT GAAAATCAGG ATATGAGTAC CAAAGGAAAA 
TCTTCAGATC AACTCGAAGT GTTTTTCGAG GGGTTGTGCA AATTTGCTCG GTATAGCAAG 
TTCGAAACCT GTGGGACAAT AAGAAGTGGG GACCTTTTAA ACTCTGCAAG TGTGGTCTGC 
TCGTTGAGTT TTGACCCTGA TGAAGAACAC ATAGCAGCAG CTGGGATATC AAAGAAGATC 
AAGATTTTTG ACTTCAATGC ATTTATGAAT GAATCTGTCG GTGTTCATTA TCCACTAGTA 
GAGATGGTCA ACAAATCTAA ATTAAGCTGT GTTTGCTGGA ATAGCTACAT CAAAAACTAC 
TTGGCCTCGA CTGACTACGA TGGTGTAGTT CAGATATGGG ATGCCGGCAC TGGACAAGGA 
TTTTCGCAGT ACACAGAACA CCAGAAGAGA GCCTGGTCAG TTGATTTTTC TCCATCTGAC 
CCGACAAAAT TTGTTAGTGG AAGTGATGAC TGCTCGGTGA AGCTTTGGAG CATCAATGAG 
AAACGTTCAT TAGGCACAAT CTGGAGTCCA GCAAATGTGT GTTGCGTGCA ATTCTCTTCT 
TATTCCAATC ACTTATTGGC ATTTGGGTCA GCTGATTACA AGGTCTATTG CTATGACCTT 
CGGTATGTCA AAACACCTTG GTGCACATTG GCTGGTCATG AGAAAGCGGT GAGTTATGTT 
AAATTTATGG ATTCAGAAAC CATTGTTTCT GCTTCCACTG ATAACTCTCT CAAGCTTTGG 
AATCTTAACA AGACAAATTC CTCTGGTTTA TCTCCTGGTG CTTGTTCATT GACATATAAA 
GGACATACAA ATCAGAAGAA TTTCGTTGGA TTGTCGGTTT TGGACGGATA CATAGCTTGT 
GGTTCAGAGA CCAATGAGGT ATATAGTTAC TATAAATCTT TACCGATGCC AATGACTTCA 
TACAAGTTTG GATCCGTCGA TCCCATTTCT GGAAACGAAT ACTTTGATGA CAATGGACAG 
TTTGTGTCGA GCGTCTGTTG GAGGAAAAAA TCGAATATGC TTGTAGCTGC AAACTCTACT 
GGAAACATGA AGCTACTAAA ACTTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

SPA1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報