ISA1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ISA1
Entrez Gene ID	818580
Full Name	isoamylase 1
Synonyms	ARABIDOPSIS THALIANA ISOAMYLASE 1,ATISA1,T28M21.9,T28M21_9,isoamylase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001336807.1	NP_001324597.1	isoamylase 1
NM_129551.4	NP_181522.1	isoamylase 1
NM_001336808.1	NP_001324596.1	isoamylase 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os08g0520900	NP_001062271.1
Arabidopsis thaliana (thale cress)	ISA1	NP_181522.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endogenous starch debranching enzymes ISA1 and ISA2 of Arabidopsis are conserved among diverse groups of plants.
Title: Replacement of the endogenous starch debranching enzymes ISA1 and ISA2 of Arabidopsis with the rice orthologs reveals a degree of functional conservation during starch synthesis.

Data indicate that analysis of recombinant enzymes showed that Data iindicate that Arabidopsis ISA1 requires ISA2 as a partner for enzymatic function, whereas maize ISA1 was active by itself.
Title: Distinct functional properties of isoamylase-type starch debranching enzymes in monocot and dicot leaves.

The heteromultimeric debranching enzyme involved in starch synthesis in Arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity.
Title: The heteromultimeric debranching enzyme involved in starch synthesis in Arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity.

Data propose that ISA1 function in normal amylopectin synthesis by promoting amylopectin crystallization but conclude that they are not mandatory for starch granule synthesis.
Title: Starch granule biosynthesis in Arabidopsis is abolished by removal of all debranching enzymes but restored by the subsequent removal of an endoamylase.

The following ISA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb09280	NM_129551.4 Latest version!	Arabidopsis thaliana isoamylase 1 (ISA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb36463	NM_001336807.1 Latest version!	Arabidopsis thaliana isoamylase 1 (ISA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAb36464	NM_001336808.1 Latest version!	Arabidopsis thaliana isoamylase 1 (ISA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ISA1 ( NM_129551.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_181522.1 Arabidopsis thaliana isoamylase 1 (ISA1), mRNA.

Price & Availability↑

CloneID	OAb09280
Clone ID Related Accession (Same CDS sequence)	NM_129551.4
Accession Version	NM_129551.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	isoamylase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_129551.3.

ATGGATGCAA TCAAATGCAG TTCCAGTTTC CTCCATCACA CCAAGCTCAA CACTCTCTTC 
TCTAATCACA CCTTTCCTAA AATCTCCGCT CCCAATTTCA AGCCCCTCTT CCGTCCGATT 
TCGATTTCCG CAAAGGACAG AAGAAGCAAC GAAGCTGAAA ATATCGCCGT AGTAGAGAAA 
CCTCTTAAAT CAGATAGATT TTTTATCTCC GATGGACTTC CTTCTCCATT CGGACCCACC 
GTCAGAGACG ACGGCGTCAA TTTCTCTGTT TACTCTACAA ACTCCGTTTC CGCTACCATC 
TGCTTGATTT CTCTCTCCGA TCTCCGTCAG AACAAAGTGA CGGAGGAGAT TCAGCTTGAT 
CCATCAAGGA ATAGAACTGG CCATGTTTGG CATGTGTTCT TGAGAGGAGA TTTCAAAGAT 
ATGTTGTATG GTTATAGATT TGATGGCAAG TTTTCTCCTG AAGAAGGTCA TTATTATGAT 
TCCTCCAACA TTTTATTGGA TCCTTACGCA AAGGCAATTA TAAGCAGAGA TGAGTTTGGA 
GTTTTGGGAC CTGATGATAA TTGTTGGCCT CAAATGGCGT GTATGGTACC CACTCGTGAG 
GAAGAGTTTG ATTGGGAAGG GGATATGCAT CTGAAGCTTC CACAGAAAGA TCTTGTTATA 
TATGAAATGC ATGTGCGAGG TTTTACAAGG CATGAGTCTA GTAAAATTGA ATTCCCTGGC 
ACATACCAGG GTGTTGCAGA GAAGCTTGAC CATTTGAAGG AGCTTGGGAT AAATTGTATA 
GAATTAATGC CATGTCACGA GTTTAATGAG CTGGAGTATT ACAGCTACAA TACGATTTTG 
GGAGACCACA GGGTAAATTT TTGGGGTTAC TCTACCATTG GGTTCTTCTC GCCCATGATC 
AGATACGCAT CAGCAAGCTC TAACAATTTT GCTGGACGAG CCATAAATGA ATTCAAAATT 
CTTGTTAAGG AGGCACATAA ACGAGGAATT GAGGTAATCA TGGATGTCGT CTTGAACCAC 
ACAGCCGAAG GGAATGAAAA AGGGCCCATT TTCTCATTTA GAGGAGTTGA TAACAGTGTC 
TATTACATGC TTGCTCCAAA GGGCGAGTTC TATAATTATT CAGGCTGTGG TAATACATTC 
AACTGCAATC ATCCTGTGGT GCGTCAATTC ATATTGGATT GCCTGAGATA TTGGGTTACA 
GAAATGCATG TTGACGGCTT CCGCTTTGAT CTTGGTTCAA TCATGTCAAG GAGCAGCAGC 
CTTTGGGATG CAGCCAATGT TTACGGGGCT GATGTAGAAG GTGACTTGCT CACAACTGGT 
ACTCCTATTA GCTGCCCTCC AGTAATTGAC ATGATAAGTA ATGATCCAAT ACTCCGCGGT 
GTTAAGCTAA TAGCTGAAGC ATGGGATGCG GGTGGGCTGT ACCAAGTTGG CATGTTTCCA 
CACTGGGGTA TTTGGTCTGA GTGGAATGGA AAGTTTCGGG ATGTTGTGAG ACAGTTCATA 
AAAGGCACCG ATGGCTTTTC TGGTGCTTTT GCTGAATGTC TCTGTGGAAG CCCAAATCTG 
TACCAGGGAG GTAGGAAACC TTGGCACAGC ATCAATTTTA TATGTGCGCA TGATGGTTTT 
ACGTTGGCAG ATTTAGTAAC TTACAACAAT AAGAATAACT TGGCAAATGG AGAAGAGAAT 
AATGATGGAG AGAATCACAA TTACAGCTGG AACTGTGGAG AGGAGGGAGA CTTTGCGAGT 
ATCTCGGTGA AGAGACTAAG GAAACGACAG ATGCGGAATT TCTTTGTTTC CCTCATGGTT 
TCCCAAGGTG TCCCAATGAT TTACATGGGA GATGAATATG GCCATACTAA AGGGGGAAAC 
AACAACACGT ATTGCCATGA CAACTATATG AACTATTTTC GGTGGGATAA AAAGGAAGAA 
GCACATTCTG ACTTCTTCAG ATTCTGCCGT ATTCTTATCA AGTTTCGTGA TGAATGTGAA 
TCACTTGGCT TGAATGATTT CCCAACAGCA AAGCGTCTGC AGTGGCATGG TCTTGCTCCT 
GAGATCCCAA ATTGGTCTGA AACAAGTCGA TTTGTTGCAT TTTCACTGGT CGATTCTGTG 
AAGAAAGAAA TCTATGTGGC CTTCAACACC AGTCATTTAG CCACACTTGT TTCGCTACCG 
AATAGGCCAG GATACCGATG GGAGCCATTT GTAGACACGA GCAAACCGAG CCCTTACGAC 
TGCATAACAC CGGATCTCCC AGAGAGAGAA ACGGCGATGA AGCAGTATAG GCACTTCTTA 
GATGCAAATG TGTACCCAAT GCTCAGTTAC TCATCCATCA TTCTCCTTCT TTCACCAATT 
AAAGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_181522.1
CDS	228..2579
Translation	MDAIKCSSSFLHHTKLNTLFSNHTFPKISAPNFKPLFRPISISAKDRRSNEAENIAVVEKPLKSDRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSVKKEIYVAFNTSHLATLVSLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFLDANVYPMLSYSSIILLLSPIKDP

Target ORF information:

RefSeq Version	NM_129551.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana isoamylase 1 (ISA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_129551.4

ORF Insert Sequence:

ATGGATGCAA TCAAATGCAG TTCCAGTTTC CTCCATCACA CCAAGCTCAA CACTCTCTTC 
TCTAATCACA CCTTTCCTAA AATCTCCGCT CCCAATTTCA AGCCCCTCTT CCGTCCGATT 
TCGATTTCCG CAAAGGACAG AAGAAGCAAC GAAGCTGAAA ATATCGCCGT AGTAGAGAAA 
CCTCTTAAAT CAGATAGATT TTTTATCTCC GATGGACTTC CTTCTCCATT CGGACCCACC 
GTCAGAGACG ACGGCGTCAA TTTCTCTGTT TACTCTACAA ACTCCGTTTC CGCTACCATC 
TGCTTGATTT CTCTCTCCGA TCTCCGTCAG AACAAAGTGA CGGAGGAGAT TCAGCTTGAT 
CCATCAAGGA ATAGAACTGG CCATGTTTGG CATGTGTTCT TGAGAGGAGA TTTCAAAGAT 
ATGTTGTATG GTTATAGATT TGATGGCAAG TTTTCTCCTG AAGAAGGTCA TTATTATGAT 
TCCTCCAACA TTTTATTGGA TCCTTACGCA AAGGCAATTA TAAGCAGAGA TGAGTTTGGA 
GTTTTGGGAC CTGATGATAA TTGTTGGCCT CAAATGGCGT GTATGGTACC CACTCGTGAG 
GAAGAGTTTG ATTGGGAAGG GGATATGCAT CTGAAGCTTC CACAGAAAGA TCTTGTTATA 
TATGAAATGC ATGTGCGAGG TTTTACAAGG CATGAGTCTA GTAAAATTGA ATTCCCTGGC 
ACATACCAGG GTGTTGCAGA GAAGCTTGAC CATTTGAAGG AGCTTGGGAT AAATTGTATA 
GAATTAATGC CATGTCACGA GTTTAATGAG CTGGAGTATT ACAGCTACAA TACGATTTTG 
GGAGACCACA GGGTAAATTT TTGGGGTTAC TCTACCATTG GGTTCTTCTC GCCCATGATC 
AGATACGCAT CAGCAAGCTC TAACAATTTT GCTGGACGAG CCATAAATGA ATTCAAAATT 
CTTGTTAAGG AGGCACATAA ACGAGGAATT GAGGTAATCA TGGATGTCGT CTTGAACCAC 
ACAGCCGAAG GGAATGAAAA AGGGCCCATT TTCTCATTTA GAGGAGTTGA TAACAGTGTC 
TATTACATGC TTGCTCCAAA GGGCGAGTTC TATAATTATT CAGGCTGTGG TAATACATTC 
AACTGCAATC ATCCTGTGGT GCGTCAATTC ATATTGGATT GCCTGAGATA TTGGGTTACA 
GAAATGCATG TTGACGGCTT CCGCTTTGAT CTTGGTTCAA TCATGTCAAG GAGCAGCAGC 
CTTTGGGATG CAGCCAATGT TTACGGGGCT GATGTAGAAG GTGACTTGCT CACAACTGGT 
ACTCCTATTA GCTGCCCTCC AGTAATTGAC ATGATAAGTA ATGATCCAAT ACTCCGCGGT 
GTTAAGCTAA TAGCTGAAGC ATGGGATGCG GGTGGGCTGT ACCAAGTTGG CATGTTTCCA 
CACTGGGGTA TTTGGTCTGA GTGGAATGGA AAGTTTCGGG ATGTTGTGAG ACAGTTCATA 
AAAGGCACCG ATGGCTTTTC TGGTGCTTTT GCTGAATGTC TCTGTGGAAG CCCAAATCTG 
TACCAGGGAG GTAGGAAACC TTGGCACAGC ATCAATTTTA TATGTGCGCA TGATGGTTTT 
ACGTTGGCAG ATTTAGTAAC TTACAACAAT AAGAATAACT TGGCAAATGG AGAAGAGAAT 
AATGATGGAG AGAATCACAA TTACAGCTGG AACTGTGGAG AGGAGGGAGA CTTTGCGAGT 
ATCTCGGTGA AGAGACTAAG GAAACGACAG ATGCGGAATT TCTTTGTTTC CCTCATGGTT 
TCCCAAGGTG TCCCAATGAT TTACATGGGA GATGAATATG GCCATACTAA AGGGGGAAAC 
AACAACACGT ATTGCCATGA CAACTATATG AACTATTTTC GGTGGGATAA AAAGGAAGAA 
GCACATTCTG ACTTCTTCAG ATTCTGCCGT ATTCTTATCA AGTTTCGTGA TGAATGTGAA 
TCACTTGGCT TGAATGATTT CCCAACAGCA AAGCGTCTGC AGTGGCATGG TCTTGCTCCT 
GAGATCCCAA ATTGGTCTGA AACAAGTCGA TTTGTTGCAT TTTCACTGGT CGATTCTGTG 
AAGAAAGAAA TCTATGTGGC CTTCAACACC AGTCATTTAG CCACACTTGT TTCGCTACCG 
AATAGGCCAG GATACCGATG GGAGCCATTT GTAGACACGA GCAAACCGAG CCCTTACGAC 
TGCATAACAC CGGATCTCCC AGAGAGAGAA ACGGCGATGA AGCAGTATAG GCACTTCTTA 
GATGCAAATG TGTACCCAAT GCTCAGTTAC TCATCCATCA TTCTCCTTCT TTCACCAATT 
AAAGACCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISA1 ( NM_001336807.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001324597.1 Arabidopsis thaliana isoamylase 1 (ISA1), partial mRNA.

Price & Availability↑

CloneID	OAb36463
Clone ID Related Accession (Same CDS sequence)	NM_001336807.1
Accession Version	NM_001336807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	isoamylase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). COMPLETENESS: incomplete on the 3' end.

ATGGATGCAA TCAAATGCAG TTCCAGTTTC CTCCATCACA CCAAGCTCAA CACTCTCTTC 
TCTAATCACA CCTTTCCTAA AATCTCCGCT CCCAATTTCA AGCCCCTCTT CCGTCCGATT 
TCGATTTCCG CAAAGGACAG AAGAAGCAAC GAAGCTGAAA ATATCGCCGT AGTAGAGAAA 
CCTCTTAAAT CAGATAGATT TTTTATCTCC GATGGACTTC CTTCTCCATT CGGACCCACC 
GTCAGAGACG ACGGCGTCAA TTTCTCTGTT TACTCTACAA ACTCCGTTTC CGCTACCATC 
TGCTTGATTT CTCTCTCCGA TCTCCGTCAG AACAAAGTGA CGGAGGAGAT TCAGCTTGAT 
CCATCAAGGA ATAGAACTGG CCATGTTTGG CATGTGTTCT TGAGAGGAGA TTTCAAAGAT 
ATGTTGTATG GTTATAGATT TGATGGCAAG TTTTCTCCTG AAGAAGGTCA TTATTATGAT 
TCCTCCAACA TTTTATTGGA TCCTTACGCA AAGGCAATTA TAAGCAGAGA TGAGTTTGGA 
GTTTTGGGAC CTGATGATAA TTGTTGGCCT CAAATGGCGT GTATGGTACC CACTCGTGAG 
GAAGAGTTTG ATTGGGAAGG GGATATGCAT CTGAAGCTTC CACAGAAAGA TCTTGTTATA 
TATGAAATGC ATGTGCGAGG TTTTACAAGG CATGAGTCTA GTAAAATTGA ATTCCCTGGC 
ACATACCAGG GTGTTGCAGA GAAGCTTGAC CATTTGAAGG AGCTTGGGAT AAATTGTATA 
GAATTAATGC CATGTCACGA GTTTAATGAG CTGGAGTATT ACAGCTACAA TACGATTTTG 
GGAGACCACA GGGTAAATTT TTGGGGTTAC TCTACCATTG GGTTCTTCTC GCCCATGATC 
AGATACGCAT CAGCAAGCTC TAACAATTTT GCTGGACGAG CCATAAATGA ATTCAAAATT 
CTTGTTAAGG AGGCACATAA ACGAGGAATT GAGGTAATCA TGGATGTCGT CTTGAACCAC 
ACAGCCGAAG GGAATGAAAA AGGGCCCATT TTCTCATTTA GAGGAGTTGA TAACAGTGTC 
TATTACATGC TTGCTCCAAA GGGCGAGTTC TATAATTATT CAGGCTGTGG TAATACATTC 
AACTGCAATC ATCCTGTGGT GCGTCAATTC ATATTGGATT GCCTGAGATA TTGGGTTACA 
GAAATGCATG TTGACGGCTT CCGCTTTGAT CTTGGTTCAA TCATGTCAAG GAGCAGCAGC 
CTTTGGGATG CAGCCAATGT TTACGGGGCT GATGTAGAAG GTGACTTGCT CACAACTGGT 
ACTCCTATTA GCTGCCCTCC AGTAATTGAC ATGATAAGTA ATGATCCAAT ACTCCGCGGT 
GTTAAGCTAA TAGCTGAAGC ATGGGATGCG GGTGGGCTGT ACCAAGTTGG CATGTTTCCA 
CACTGGGGTA TTTGGTCTGA GTGGAATGGA AAGTTTCGGG ATGTTGTGAG ACAGTTCATA 
AAAGGCACCG ATGGCTTTTC TGGTGCTTTT GCTGAATGTC TCTGTGGAAG CCCAAATCTG 
TACCAGGGAG GTAGGAAACC TTGGCACAGC ATCAATTTTA TATGTGCGCA TGATGGTTTT 
ACGTTGGCAG ATTTAGTAAC TTACAACAAT AAGAATAACT TGGCAAATGG AGAAGAGAAT 
AATGATGGAG AGAATCACAA TTACAGCTGG AACTGTGGAG AGGAGGGAGA CTTTGCGAGT 
ATCTCGGTGA AGAGACTAAG GAAACGACAG ATGCGGAATT TCTTTGTTTC CCTCATGGTT 
TCCCAAGGTG TCCCAATGAT TTACATGGGA GATGAATATG GCCATACTAA AGGGGGAAAC 
AACAACACGT ATTGCCATGA CAACTATATG AACTATTTTC GGTGGGATAA AAAGGAAGAA 
GCACATTCTG ACTTCTTCAG ATTCTGCCGT ATTCTTATCA AGTTTCGTGA GTATGTAATC 
TTCTTTCATG AGTCTATCGA TCTTAATTGC TTAGTTGATC GCTTCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001324597.1
CDS	56..2086
Translation	MDAIKCSSSFLHHTKLNTLFSNHTFPKISAPNFKPLFRPISISAKDRRSNEAENIAVVEKPLKSDRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFREYVIFFHESIDLNCLVDRFT

Target ORF information:

RefSeq Version	NM_001336807.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana isoamylase 1 (ISA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336807.1

ORF Insert Sequence:

ATGGATGCAA TCAAATGCAG TTCCAGTTTC CTCCATCACA CCAAGCTCAA CACTCTCTTC 
TCTAATCACA CCTTTCCTAA AATCTCCGCT CCCAATTTCA AGCCCCTCTT CCGTCCGATT 
TCGATTTCCG CAAAGGACAG AAGAAGCAAC GAAGCTGAAA ATATCGCCGT AGTAGAGAAA 
CCTCTTAAAT CAGATAGATT TTTTATCTCC GATGGACTTC CTTCTCCATT CGGACCCACC 
GTCAGAGACG ACGGCGTCAA TTTCTCTGTT TACTCTACAA ACTCCGTTTC CGCTACCATC 
TGCTTGATTT CTCTCTCCGA TCTCCGTCAG AACAAAGTGA CGGAGGAGAT TCAGCTTGAT 
CCATCAAGGA ATAGAACTGG CCATGTTTGG CATGTGTTCT TGAGAGGAGA TTTCAAAGAT 
ATGTTGTATG GTTATAGATT TGATGGCAAG TTTTCTCCTG AAGAAGGTCA TTATTATGAT 
TCCTCCAACA TTTTATTGGA TCCTTACGCA AAGGCAATTA TAAGCAGAGA TGAGTTTGGA 
GTTTTGGGAC CTGATGATAA TTGTTGGCCT CAAATGGCGT GTATGGTACC CACTCGTGAG 
GAAGAGTTTG ATTGGGAAGG GGATATGCAT CTGAAGCTTC CACAGAAAGA TCTTGTTATA 
TATGAAATGC ATGTGCGAGG TTTTACAAGG CATGAGTCTA GTAAAATTGA ATTCCCTGGC 
ACATACCAGG GTGTTGCAGA GAAGCTTGAC CATTTGAAGG AGCTTGGGAT AAATTGTATA 
GAATTAATGC CATGTCACGA GTTTAATGAG CTGGAGTATT ACAGCTACAA TACGATTTTG 
GGAGACCACA GGGTAAATTT TTGGGGTTAC TCTACCATTG GGTTCTTCTC GCCCATGATC 
AGATACGCAT CAGCAAGCTC TAACAATTTT GCTGGACGAG CCATAAATGA ATTCAAAATT 
CTTGTTAAGG AGGCACATAA ACGAGGAATT GAGGTAATCA TGGATGTCGT CTTGAACCAC 
ACAGCCGAAG GGAATGAAAA AGGGCCCATT TTCTCATTTA GAGGAGTTGA TAACAGTGTC 
TATTACATGC TTGCTCCAAA GGGCGAGTTC TATAATTATT CAGGCTGTGG TAATACATTC 
AACTGCAATC ATCCTGTGGT GCGTCAATTC ATATTGGATT GCCTGAGATA TTGGGTTACA 
GAAATGCATG TTGACGGCTT CCGCTTTGAT CTTGGTTCAA TCATGTCAAG GAGCAGCAGC 
CTTTGGGATG CAGCCAATGT TTACGGGGCT GATGTAGAAG GTGACTTGCT CACAACTGGT 
ACTCCTATTA GCTGCCCTCC AGTAATTGAC ATGATAAGTA ATGATCCAAT ACTCCGCGGT 
GTTAAGCTAA TAGCTGAAGC ATGGGATGCG GGTGGGCTGT ACCAAGTTGG CATGTTTCCA 
CACTGGGGTA TTTGGTCTGA GTGGAATGGA AAGTTTCGGG ATGTTGTGAG ACAGTTCATA 
AAAGGCACCG ATGGCTTTTC TGGTGCTTTT GCTGAATGTC TCTGTGGAAG CCCAAATCTG 
TACCAGGGAG GTAGGAAACC TTGGCACAGC ATCAATTTTA TATGTGCGCA TGATGGTTTT 
ACGTTGGCAG ATTTAGTAAC TTACAACAAT AAGAATAACT TGGCAAATGG AGAAGAGAAT 
AATGATGGAG AGAATCACAA TTACAGCTGG AACTGTGGAG AGGAGGGAGA CTTTGCGAGT 
ATCTCGGTGA AGAGACTAAG GAAACGACAG ATGCGGAATT TCTTTGTTTC CCTCATGGTT 
TCCCAAGGTG TCCCAATGAT TTACATGGGA GATGAATATG GCCATACTAA AGGGGGAAAC 
AACAACACGT ATTGCCATGA CAACTATATG AACTATTTTC GGTGGGATAA AAAGGAAGAA 
GCACATTCTG ACTTCTTCAG ATTCTGCCGT ATTCTTATCA AGTTTCGTGA GTATGTAATC 
TTCTTTCATG AGTCTATCGA TCTTAATTGC TTAGTTGATC GCTTCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISA1 ( NM_001336808.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001324596.1 Arabidopsis thaliana isoamylase 1 (ISA1), partial mRNA.

Price & Availability↑

CloneID	OAb36464
Clone ID Related Accession (Same CDS sequence)	NM_001336808.1
Accession Version	NM_001336808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	isoamylase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). COMPLETENESS: incomplete on the 3' end.

ATGGATGCAA TCAAATGCAG TTCCAGTTTC CTCCATCACA CCAAGCTCAA CACTCTCTTC 
TCTAATCACA CCTTTCCTAA AATCTCCGCT CCCAATTTCA AGCCCCTCTT CCGTCCGATT 
TCGATTTCCG CAAAGGACAG AAGAAGCAAC GAAGCTGAAA ATATCGCCGT AGTAGAGAAA 
CCTCTTAAAT CAGATAGATT TTTTATCTCC GATGGACTTC CTTCTCCATT CGGACCCACC 
GTCAGAGACG ACGGCGTCAA TTTCTCTGTT TACTCTACAA ACTCCGTTTC CGCTACCATC 
TGCTTGATTT CTCTCTCCGA TCTCCGTCAG AACAAAGTGA CGGAGGAGAT TCAGCTTGAT 
CCATCAAGGA ATAGAACTGG CCATGTTTGG CATGTGTTCT TGAGAGGAGA TTTCAAAGAT 
ATGTTGTATG GTTATAGATT TGATGGCAAG TTTTCTCCTG AAGAAGGTCA TTATTATGAT 
TCCTCCAACA TTTTATTGGA TCCTTACGCA AAGGCAATTA TAAGCAGAGA TGAGTTTGGA 
GTTTTGGGAC CTGATGATAA TTGTTGGCCT CAAATGGCGT GTATGGTACC CACTCGTGAG 
GAAGAGTTTG ATTGGGAAGG GGATATGCAT CTGAAGCTTC CACAGAAAGA TCTTGTTATA 
TATGAAATGC ATGTGCGAGG TTTTACAAGG CATGAGTCTA GTAAAATTGA ATTCCCTGGC 
ACATACCAGG GTGTTGCAGA GAAGCTTGAC CATTTGAAGG AGCTTGGGAT AAATTGTATA 
GAATTAATGC CATGTCACGA GTTTAATGAG CTGGAGTATT ACAGCTACAA TACGATTTTG 
GGAGACCACA GGGTAAATTT TTGGGGTTAC TCTACCATTG GGTTCTTCTC GCCCATGATC 
AGATACGCAT CAGCAAGCTC TAACAATTTT GCTGGACGAG CCATAAATGA ATTCAAAATT 
CTTGTTAAGG AGGCACATAA ACGAGGAATT GAGGTAATCA TGGATGTCGT CTTGAACCAC 
ACAGCCGAAG GGAATGAAAA AGGGCCCATT TTCTCATTTA GAGGAGTTGA TAACAGTGTC 
TATTACATGC TTGCTCCAAA GGGCGAGTTC TATAATTATT CAGGCTGTGG TAATACATTC 
AACTGCAATC ATCCTGTGGT GCGTCAATTC ATATTGGATT GCCTGAGATA TTGGGTTACA 
GAAATGCATG TTGACGGCTT CCGCTTTGAT CTTGGTTCAA TCATGTCAAG GAGCAGCAGC 
CTTTGGGATG CAGCCAATGT TTACGGGGCT GATGTAGAAG GTGACTTGCT CACAACTGGT 
ACTCCTATTA GCTGCCCTCC AGTAATTGAC ATGATAAGTA ATGATCCAAT ACTCCGCGGT 
GTTAAGCTAA TAGCTGAAGC ATGGGATGCG GGTGGGCTGT ACCAAGTTGG CATGTTTCCA 
CACTGGGGTA TTTGGTCTGA GTGGAATGGA AAGTTTCGGG ATGTTGTGAG ACAGTTCATA 
AAAGGCACCG ATGGCTTTTC TGGTGCTTTT GCTGAATGTC TCTGTGGAAG CCCAAATCTG 
TACCAGGGAG GTAGGAAACC TTGGCACAGC ATCAATTTTA TATGTGCGCA TGATGGTTTT 
ACGTTGGCAG ATTTAGTAAC TTACAACAAT AAGAATAACT TGGCAAATGG AGAAGAGAAT 
AATGATGGAG AGAATCACAA TTACAGCTGG AACTGTGGAG AGGAGGGAGA CTTTGCGAGT 
ATCTCGGTGA AGAGACTAAG GAAACGACAG ATGCGGAATT TCTTTGTTTC CCTCATGGTT 
TCCCAAGTAA AGCTAACCAT TTGTTGCTTC TTTAATTTTA ATTCCTGCTC AAAGCTCGTT 
TCACTGATGT TATGCACATT GAACTATGAT ACTGGAGTAG AGGGCATGAG TTCAAAATAT 
AAATTAATAA GCCACAAGAG AATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001324596.1
CDS	56..2002
Translation	MDAIKCSSSFLHHTKLNTLFSNHTFPKISAPNFKPLFRPISISAKDRRSNEAENIAVVEKPLKSDRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQVKLTICCFFNFNSCSKLVSLMLCTLNYDTGVEGMSSKYKLISHKRI

Target ORF information:

RefSeq Version	NM_001336808.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana isoamylase 1 (ISA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336808.1

ORF Insert Sequence:

ATGGATGCAA TCAAATGCAG TTCCAGTTTC CTCCATCACA CCAAGCTCAA CACTCTCTTC 
TCTAATCACA CCTTTCCTAA AATCTCCGCT CCCAATTTCA AGCCCCTCTT CCGTCCGATT 
TCGATTTCCG CAAAGGACAG AAGAAGCAAC GAAGCTGAAA ATATCGCCGT AGTAGAGAAA 
CCTCTTAAAT CAGATAGATT TTTTATCTCC GATGGACTTC CTTCTCCATT CGGACCCACC 
GTCAGAGACG ACGGCGTCAA TTTCTCTGTT TACTCTACAA ACTCCGTTTC CGCTACCATC 
TGCTTGATTT CTCTCTCCGA TCTCCGTCAG AACAAAGTGA CGGAGGAGAT TCAGCTTGAT 
CCATCAAGGA ATAGAACTGG CCATGTTTGG CATGTGTTCT TGAGAGGAGA TTTCAAAGAT 
ATGTTGTATG GTTATAGATT TGATGGCAAG TTTTCTCCTG AAGAAGGTCA TTATTATGAT 
TCCTCCAACA TTTTATTGGA TCCTTACGCA AAGGCAATTA TAAGCAGAGA TGAGTTTGGA 
GTTTTGGGAC CTGATGATAA TTGTTGGCCT CAAATGGCGT GTATGGTACC CACTCGTGAG 
GAAGAGTTTG ATTGGGAAGG GGATATGCAT CTGAAGCTTC CACAGAAAGA TCTTGTTATA 
TATGAAATGC ATGTGCGAGG TTTTACAAGG CATGAGTCTA GTAAAATTGA ATTCCCTGGC 
ACATACCAGG GTGTTGCAGA GAAGCTTGAC CATTTGAAGG AGCTTGGGAT AAATTGTATA 
GAATTAATGC CATGTCACGA GTTTAATGAG CTGGAGTATT ACAGCTACAA TACGATTTTG 
GGAGACCACA GGGTAAATTT TTGGGGTTAC TCTACCATTG GGTTCTTCTC GCCCATGATC 
AGATACGCAT CAGCAAGCTC TAACAATTTT GCTGGACGAG CCATAAATGA ATTCAAAATT 
CTTGTTAAGG AGGCACATAA ACGAGGAATT GAGGTAATCA TGGATGTCGT CTTGAACCAC 
ACAGCCGAAG GGAATGAAAA AGGGCCCATT TTCTCATTTA GAGGAGTTGA TAACAGTGTC 
TATTACATGC TTGCTCCAAA GGGCGAGTTC TATAATTATT CAGGCTGTGG TAATACATTC 
AACTGCAATC ATCCTGTGGT GCGTCAATTC ATATTGGATT GCCTGAGATA TTGGGTTACA 
GAAATGCATG TTGACGGCTT CCGCTTTGAT CTTGGTTCAA TCATGTCAAG GAGCAGCAGC 
CTTTGGGATG CAGCCAATGT TTACGGGGCT GATGTAGAAG GTGACTTGCT CACAACTGGT 
ACTCCTATTA GCTGCCCTCC AGTAATTGAC ATGATAAGTA ATGATCCAAT ACTCCGCGGT 
GTTAAGCTAA TAGCTGAAGC ATGGGATGCG GGTGGGCTGT ACCAAGTTGG CATGTTTCCA 
CACTGGGGTA TTTGGTCTGA GTGGAATGGA AAGTTTCGGG ATGTTGTGAG ACAGTTCATA 
AAAGGCACCG ATGGCTTTTC TGGTGCTTTT GCTGAATGTC TCTGTGGAAG CCCAAATCTG 
TACCAGGGAG GTAGGAAACC TTGGCACAGC ATCAATTTTA TATGTGCGCA TGATGGTTTT 
ACGTTGGCAG ATTTAGTAAC TTACAACAAT AAGAATAACT TGGCAAATGG AGAAGAGAAT 
AATGATGGAG AGAATCACAA TTACAGCTGG AACTGTGGAG AGGAGGGAGA CTTTGCGAGT 
ATCTCGGTGA AGAGACTAAG GAAACGACAG ATGCGGAATT TCTTTGTTTC CCTCATGGTT 
TCCCAAGTAA AGCTAACCAT TTGTTGCTTC TTTAATTTTA ATTCCTGCTC AAAGCTCGTT 
TCACTGATGT TATGCACATT GAACTATGAT ACTGGAGTAG AGGGCATGAG TTCAAAATAT 
AAATTAATAA GCCACAAGAG AATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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ISA1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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