NCU04766 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU04766
Entrez Gene ID	3876446
Full Name	Ccr4-Not transcription complex subunit
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_955206.2	XP_960299.1	Ccr4-Not transcription complex subunit

Pathways from BioSystems

Homologies

Neurospora crassa	NCU04766	XP_960299.1
Schizosaccharomyces pombe (fission yeast)	not1	NP_593323.1
Magnaporthe oryzae (rice blast fungus)	MGG_02476	XP_003709270.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU04766 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU04766 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf06038	XM_955206.2 Latest version!	Neurospora crassa OR74A Ccr4-Not transcription complex subunit (NCU04766), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU04766 ( XM_955206.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_960299.1 Neurospora crassa OR74A Ccr4-Not transcription complex subunit (NCU04766), mRNA.

Price & Availability↑

CloneID	ONf06038
Clone ID Related Accession (Same CDS sequence)	XM_955206.2
Accession Version	XM_955206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	Ccr4-Not transcription complex subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506). On Feb 23, 2015 this sequence version replaced XM_955206.1.

ATGGAAGGGT TTCGGTCTTA CTTTCAGCGG TTAGTGGTCG GCAATGCCGG CCAAATCTTT 
CCGAACCAAA AATGGCAGGG CGTCAACTCG ACCACCTACC ACCAGCTCGT CGCAGAGGTG 
AAGAAGCTAT CACAAGACTT TGAGCAGTCC AGGAGAATTG CCGAGGCGAT TGAGACAGGC 
ACAGAAGACC TCTTCCGCGA CTTTGATCTT TCCACCTTCA TGGAACATTT CAAGCTCGAT 
GCTTTTGAAA AGACTGTTCT AGCGCTTGCC TTCAAGCTTG GGAGCAAGCC CGATCTCAAG 
ACCAAGGCCG ATGCCATCTT ATCAACAAAC TTCCCCATGT TTGGCAACAT TCTCAGCAAA 
TCGTCCGGCA CACACACGAT GCTTGGACCC GATTTTATCG CTGGGTTGCT CGATCGCTTC 
ATTCAAGTCC ACCCGCCCAA TTTCAACGCG GCAGCAAAGA AGGAGTTGGA GCATAATGTG 
CAGTCTCGAT ACATCCAAGG AAATCCCGAT TCGAACCCGC TGCCTCCCTC TCAAGTGCTG 
TCCGCACTTG ATCTAATGCG ACTACTGGCA GACCGACCCC CGAATGCCTT GACTCTCTAC 
ATCCACAAGA CCGGGCAAGA CTTCACCGAG AATGAAGAGA CTTGCTATAA CTACCTCCGG 
AATCGTCCGG CCAACTGCCA GTTGAGTGAG GAGCAGGTAT CCCACGCGCT CATGTATACG 
ACCATCAGCC AAACCCCTCC GCATAACCCT TCGGTTCTTG TCACGGCTCT CAGGCGTGTT 
CTGCCAGGTA CCTTCAGATG GCAGGATGTG GTCTCGTACT TTGATCACAG CGGTGCCCGA 
ATCACTGCGC CCCAGTTTCT TCGTCTCTAC AATGCTCTGC TGCCGATAGC GCAAGACGAC 
AGCCAGGGTT TTGATATTCA GCGGCTATGG GGTGGCGAGT GGGAAAACCC AGAGACTCAA 
ATGTCCTTTG TCTGCGCATA CACCTCACTG CTGCCTGAAC AGCTGGACGC CACCACCATC 
CCGGGCCTGA AGCCGACCTT CACCTTGGAC GACTATGCAC AAGCACCCCA GATGATCCGG 
GAAACGGCCG CATATGCAGT CAGGCATCCC TTGGTATCCG AAGCGGCCAT GTCTGCCATC 
TTCAACGTCG CCCTCCACTC TACACACGCC TCCAAGTGTA CCGAAGCCAA GCGTCTTTTC 
CAGGATGTCG TCGTTCCCCA AGTGGCCATC TTTGTGGTCT CGGCCTTCAG GGTGCCCAAG 
CCTTGGCCCA AGCTAGCTGA GGACGCTCTG GTTGACTTGT TCATCGAGTC GTTATGGGCA 
GCCAAATCAC CCGAGACCGA TTTCGTGATG AATAGCCTGT GGAGGTGGGA CAAAGACTGG 
GTCAAAATGA GGTTGATAGA TGCCCACGCG CACAGGCCGA TATGCCTGCC ACAAATATTC 
GAGCATTCCA TACGGCAAGG CTGGCTCGAG GAGCTTGTCT ATCTAATCAA TGGCTTTGGG 
TTCGATCTCA CTGCTCTAGC CCACGCCGAG GGCTACCTCG ACCTGCAGGA GTGGGCACAG 
AAGAACGCAG AACGGAGTAC CGAGATGGCC TCGGCCCTTC TTCAGTTCCT GATGATCAAG 
GCGAATCTCG AACTGCAGTA TCAGCGTTCT GGGCCCGGTG CCCCTAAGCA GAGCACCACC 
CTGCAGGTTC GGACAGTGTC TGCCATGCTT CAGATCCTGG AGGAGTTTCT GCCCAAGTCG 
CCGATCCCGG AGCTTATTGT GGTCCAAAGG CAATGTATCA CCGCGTACCC GAGGCTTATC 
AATTACGGCG AAGGGTTCGA TGACATTATT GACGCCAATG GTCGTGATGG GAATTCCTTA 
CCCCAGGCTG CTAATGTTAG AATGGAGGAG CACTACAAGA AAATGTACAG CGACGAAGTC 
GCGGTACGAA CCATCGTTGA GGTTCTCGAC CAGTACAAGC ACTCGCGAGA TCCTCTCGAA 
CAAGACACCT TTGCTTGCAT GATTCACGGC CTGTTCGATG AATACGCGCA TTATGAGAAT 
TATCCTCTGG AGGCGCTCGC CACCACGGCT GTGCTCTTCG GCGGCATCAT CTCTCACAAG 
CTCGTTTCGG AGTTGCCCCT CAAGATCGGA CTTGGCATGA TCCTCGAGGC TGTCCGGGAC 
CATCAACCCC AGGAGCCCAT GTACAAGTTT GGCTTGCAAG CTCTTCTTCA ACTCCTACCT 
CGCTTACGCG AATGGCCAGG GTTTTGCACG CAGCTATTGC TCATCCCGGG TCTTCAGAAT 
ACCGAGGCCC GCAAGAAGGC CGAAGATGTT CTTCGTGAGC ACGAGGAGGA GACGATGCGC 
GCCCGCGGAG GCGCCTTACC GCTTGGCGCG ACCATGGGTA ACGACAACAT GACAAACGGT 
AACCTTGACG AGGCTCACGG TTCGGAGCAG CACCCGCCGC CATTCGCGTC GGTCAACGTT 
GATCCGCCTC CACCGGGGGT GATTTATGAG GACCCAACCG CCGAGGCCCA GGGCAAGATT 
CAGTTCTTCC TCAACAACCT TACTGAAACT ACACTGCAGA CCATGTACAA GGACCTTCGC 
GACATGTTGG AGACCAAGCA TCAGCAGTGG TTCGCTAGCC ACCTGGTTGA AGAACGTGCC 
AAGATGCAGC CGAACTACCA TCAAGTTTAC CTTGACCTGG TAAAGATGTT TGAGGATAAG 
TCACTGTGGG CCGAGGTTTT GAGGGAGACC TATGTCAGCG TCCAGAGAAT GCTCAATTCC 
GACGTTACGC TACAGAACTC AACTGAGAGG ACGCACTTGA AGAATTTGGG CGGCTGGCTC 
GGTCTTTTGA CGCTCGCGAG AAACAAGCCC ATTAAGCATA GAAATATCGC TTTCAAACAG 
TTGCTTATGG AAGCGCATGA TACTAAGCGT CTCCTGATTG TTATACCTTT CGTCTGCAAG 
GTCCTCGCTC AAGGAGCTTC ATCAGAGGTC TTCCAGCCAC CCAATCCCTG GCTGATGGAC 
ATTATCCATC TCCTGATCGA GCTGTACCAC AACGCCGAGC TGAAGCTGAA CTTGAAGTTC 
GAGATTGAGG TCTTGTGCAA GGGCCTCAAG CTGGACCACA AGAGCATTGA GCCGTCTGGC 
GAGATTCTCA ACCGGGTTCC TGTCGAGGAA GTTCCTGATC TGAATGGGCA AGAGCTTGAG 
CCCTACGACA ACACCTCTAT TAACGGTATG GGTAGTGTCG CGGGTGGCCT CCCCGCTCAT 
GCCTCTCTTC CGATCCCCGA CCTTTCCGCC AGCCTCTCGA TCCCGCCAAC CGAAGTCATT 
GATCAAGCCA AACTGCACAG CATTACCTCT CAAGCAGTTA CGCGAGCCCT GCAGGAGATC 
ATCCAGCCAG TGGTTGACAG GTCCGTGACC ATTGCGGCGA TAGCCACACA GCAAATGATC 
CACAAGGACT TTGCCACCGA GCCTGATGAG AACCGGGTGC GGGACGCGGC GATCAACATG 
GTCAAGTCAA CTGCGGGCAG TCTTGCCCTG GTCACGTCCA AGGAACCCCT TCGCTCCAAC 
ATGGCCAACT ACCTTCGTGC TCTCTCGGCT GAGATCGGTC TAGTGCTGCC TGAGGGTATC 
ATCATGCTCT GCATCAACTC GAATTTGGAT CTCGCTTCTA GCGTAATCGA GAAGTCGGCT 
GAAGACCGTG CGGTTCCGGA GATCGAGGAG ATGATTCAAC CTGAAATTGA AGCCAGACGC 
CGGCATCGTG CGCAGCGTCC GAATGAGCCA TACATGGATC CTGGACTCAC TCGCTGGGCC 
TGGACCATTC CTCATCCCTT CAAGTTGAAC CCAAGCATGA CTGGTCTTAA CGCGGAGCAA 
ATGGCTATTT ACGATGACTT TGCCCGACAG CCGAGGGCAG TCGCTTCGAC GACAGGACCG 
TCCCACGCAG CCTCCACATC GGATGCCAGG TCTCTCGCAA ATGAAGTCCT CGGAGATCAG 
TTCAACACCA TTTCGGCCCT TCCCACACCT GCGGAGACAC CTTCGCTTCC TCACCTCGGT 
GGTCAGCTTC AGCACTACCC CCAGGGAAGT GGCGGATTGG CAAACGGACG TCAAGCCGGG 
CTTAACCAGG TGGATGCCAG GACCATTGCT GACAGAGTCA ACAAGCTTTT GGAGTCACTG 
ACCGCGGCCG CCACTGGTGC CACCGAGGAA CACTTCAATG AACTGCCCCG CGCTCACCAA 
GTCCTGGACA TCATTGACGC TCTTGTGCAA CTCATTATCA AGACTCAGCA GACGTCTGAA 
GAGTTTGCAG CATATGCCGC AACCCAGATC GCCCAGTTGA TCTTCAGACA GCCAGGCGAT 
ACCCTCTTGC TTGAAAGTCT CGTCCATGTC CTCGAAACCC TCCGTAAGAT TGCCGGTCCG 
GCCACGAGCC AGCAGATCAG GAGTCTCTTC CATCAACAAC CAGCGGAATA CTTCCTCAAC 
CTTTCGCTCA TTGCAGCACT GCTCGGCACG GACCTGCTCG ACTGGAGAAG CATTGATGCA 
GCCATGTCTA TGGCCCTCCA GCAACGCAAA GAGGGATCCG TCGAGTTCCT CGAGCAGCTT 
GTGGATCTTA CGCTGCTGAG TGAGAGCCCT CTTGCGCTCT ATGCTGACTT TGTCCGCAGT 
TTGGAGGAAG CATGGGCCTG GATCAGTGAG AATCCGGATG TTCCAGGAGG CCAGAGTCTC 
AAGTCCAAGA TCCTTGCCCC TCCTCCGGAC TTGCCTGAAG GCCTCACTCC GGCAGAGATC 
CTCGCCATCC GCCAGGACCA GATGGAGTAT GTCTTTGACG AATGGGTTCA CCTTTGCAAC 
AACCCTGCCG CCTCCTCCTC CAAGTCATCG ACGATCTTTG TACAGCAGAT GCGCTCCAGA 
GGAGTCATTG CTAGCACTGA TGATTTCCTC ATGTTTGCAC GCCTAGCCAT TGACAAGTCT 
GTGGACCGCT TCGAGCATGT TGTTCACCTT AATGGAACGA TAACGGAGGC GTACCAGGCG 
ATTGATGCTT TAGGCAAAAT GATCCTCATC TTCTTCCAGT CCCACGAAGA TAATGCCGAC 
GGACAAACGG CTCGTGCTGC CTTCATTGAT TCCATCCTCG CCCTTGGGGT GCTGGTATTG 
AATAGCCACC ACGTCAAGCG TGGCGACGCT TTCAACTCAC GGGTGTTTGT TCGGTTCTTC 
TCCTACCTCC TTCACGGCAT TCACTCTCTG GAGGAGCTTC CAGAAAATGA GCGTGATCGC 
TTCATGCTTA GCTTTGCCGA GCGTCTTTAC GACCTGCGGC CGAGCCTCTA CCCAGGCTTC 
GTCCTTGGCT GGCTCACACT TGTCACCCAC CGTGTCTTCC AGCCAGCCAT GCTTCAAATG 
GCTAACAACG CGGGCTGGGA GGCGTACACC AAGATCCTTC GGGATTTGCT CTCTTACCAG 
GGCGAGCTGC TGAAGCCGCT CGAGCTCTCG ATCTTCGCCA AGGAAATGTA CCAGGCCGTC 
CTAAAGCTCC TCATCATCCT GGGGCATGAT TTCCCCGATT ACGTGTCTGC CAACCATGTG 
CTGCTGTGCC AGAGCTTGCC GCCTCACGCA ACGCAATTAA GGAACATGAT CTTACTGGCT 
ACTCCTGCTT CGGCCGCGAA GCCGACCGAT GCCTTCCAGC CCGGCTTGAA ATTGGATCAG 
ATCGCAGATA TCAAAGAAGC AGCGATCAGT GTCTACGATC CTGCCGAGCA GCTACGCCAA 
CTGGGGCTGC TTGACATTCT GGATCAGGCT TTGCAGAACG GCCCGTCCGA AGATGCCATT 
GCGCAGATTA CACACGCTAT CACTAGGACT GGTAGCGGAA CGACGGACTA TGGCTTTGTT 
CCCATTGACG TCAACCGTCA AGTCATCGAC GCCGTAATCA CGCATATTGG CAACTTTGCC 
GTCAACCGCG CCGCCACCAG GAACGACGCC TCTGTGTTCG TCCCCGGCGC ACCAGACCTC 
AAGACACTGC AAATGCTCAT CACGGAACTC TCCCCCGATA CGAGGTACTT CGTTCTGAAC 
AGCATGGCGA ACTGGCTCCG CTTCACCAGC GCCCACACGC ACTATTTCAT TCAAGTCTTC 
CTTGACTTCT TTGGCCACGA CGTCGCCTCC ACGGATCCCG AGGAGATGGA CATTCGGCAG 
CAGATTGTTC GGATCCTCCT TGAACGGTTA ATAGGCTACT GGCCGTACCC GTGGGGTCTG 
ATCATCACCA CCATGGAGCT AGTCAAGAAC GAGAAGTACA ACTTCTTTGA GCTGCCTTTC 
ATCAAGGCCA CCCCTGAGGT GCGGAGAGTG TTTGAGGATA AGGTTCGCCA GCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_960299.1
CDS	631..6927
Translation	MEGFRSYFQRLVVGNAGQIFPNQKWQGVNSTTYHQLVAEVKKLSQDFEQSRRIAEAIETGTEDLFRDFDLSTFMEHFKLDAFEKTVLALAFKLGSKPDLKTKADAILSTNFPMFGNILSKSSGTHTMLGPDFIAGLLDRFIQVHPPNFNAAAKKELEHNVQSRYIQGNPDSNPLPPSQVLSALDLMRLLADRPPNALTLYIHKTGQDFTENEETCYNYLRNRPANCQLSEEQVSHALMYTTISQTPPHNPSVLVTALRRVLPGTFRWQDVVSYFDHSGARITAPQFLRLYNALLPIAQDDSQGFDIQRLWGGEWENPETQMSFVCAYTSLLPEQLDATTIPGLKPTFTLDDYAQAPQMIRETAAYAVRHPLVSEAAMSAIFNVALHSTHASKCTEAKRLFQDVVVPQVAIFVVSAFRVPKPWPKLAEDALVDLFIESLWAAKSPETDFVMNSLWRWDKDWVKMRLIDAHAHRPICLPQIFEHSIRQGWLEELVYLINGFGFDLTALAHAEGYLDLQEWAQKNAERSTEMASALLQFLMIKANLELQYQRSGPGAPKQSTTLQVRTVSAMLQILEEFLPKSPIPELIVVQRQCITAYPRLINYGEGFDDIIDANGRDGNSLPQAANVRMEEHYKKMYSDEVAVRTIVEVLDQYKHSRDPLEQDTFACMIHGLFDEYAHYENYPLEALATTAVLFGGIISHKLVSELPLKIGLGMILEAVRDHQPQEPMYKFGLQALLQLLPRLREWPGFCTQLLLIPGLQNTEARKKAEDVLREHEEETMRARGGALPLGATMGNDNMTNGNLDEAHGSEQHPPPFASVNVDPPPPGVIYEDPTAEAQGKIQFFLNNLTETTLQTMYKDLRDMLETKHQQWFASHLVEERAKMQPNYHQVYLDLVKMFEDKSLWAEVLRETYVSVQRMLNSDVTLQNSTERTHLKNLGGWLGLLTLARNKPIKHRNIAFKQLLMEAHDTKRLLIVIPFVCKVLAQGASSEVFQPPNPWLMDIIHLLIELYHNAELKLNLKFEIEVLCKGLKLDHKSIEPSGEILNRVPVEEVPDLNGQELEPYDNTSINGMGSVAGGLPAHASLPIPDLSASLSIPPTEVIDQAKLHSITSQAVTRALQEIIQPVVDRSVTIAAIATQQMIHKDFATEPDENRVRDAAINMVKSTAGSLALVTSKEPLRSNMANYLRALSAEIGLVLPEGIIMLCINSNLDLASSVIEKSAEDRAVPEIEEMIQPEIEARRRHRAQRPNEPYMDPGLTRWAWTIPHPFKLNPSMTGLNAEQMAIYDDFARQPRAVASTTGPSHAASTSDARSLANEVLGDQFNTISALPTPAETPSLPHLGGQLQHYPQGSGGLANGRQAGLNQVDARTIADRVNKLLESLTAAATGATEEHFNELPRAHQVLDIIDALVQLIIKTQQTSEEFAAYAATQIAQLIFRQPGDTLLLESLVHVLETLRKIAGPATSQQIRSLFHQQPAEYFLNLSLIAALLGTDLLDWRSIDAAMSMALQQRKEGSVEFLEQLVDLTLLSESPLALYADFVRSLEEAWAWISENPDVPGGQSLKSKILAPPPDLPEGLTPAEILAIRQDQMEYVFDEWVHLCNNPAASSSKSSTIFVQQMRSRGVIASTDDFLMFARLAIDKSVDRFEHVVHLNGTITEAYQAIDALGKMILIFFQSHEDNADGQTARAAFIDSILALGVLVLNSHHVKRGDAFNSRVFVRFFSYLLHGIHSLEELPENERDRFMLSFAERLYDLRPSLYPGFVLGWLTLVTHRVFQPAMLQMANNAGWEAYTKILRDLLSYQGELLKPLELSIFAKEMYQAVLKLLIILGHDFPDYVSANHVLLCQSLPPHATQLRNMILLATPASAAKPTDAFQPGLKLDQIADIKEAAISVYDPAEQLRQLGLLDILDQALQNGPSEDAIAQITHAITRTGSGTTDYGFVPIDVNRQVIDAVITHIGNFAVNRAATRNDASVFVPGAPDLKTLQMLITELSPDTRYFVLNSMANWLRFTSAHTHYFIQVFLDFFGHDVASTDPEEMDIRQQIVRILLERLIGYWPYPWGLIITTMELVKNEKYNFFELPFIKATPEVRRVFEDKVRQH

Target ORF information:

RefSeq Version	XM_955206.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A Ccr4-Not transcription complex subunit (NCU04766), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_955206.2

ORF Insert Sequence:

ATGGAAGGGT TTCGGTCTTA CTTTCAGCGG TTAGTGGTCG GCAATGCCGG CCAAATCTTT 
CCGAACCAAA AATGGCAGGG CGTCAACTCG ACCACCTACC ACCAGCTCGT CGCAGAGGTG 
AAGAAGCTAT CACAAGACTT TGAGCAGTCC AGGAGAATTG CCGAGGCGAT TGAGACAGGC 
ACAGAAGACC TCTTCCGCGA CTTTGATCTT TCCACCTTCA TGGAACATTT CAAGCTCGAT 
GCTTTTGAAA AGACTGTTCT AGCGCTTGCC TTCAAGCTTG GGAGCAAGCC CGATCTCAAG 
ACCAAGGCCG ATGCCATCTT ATCAACAAAC TTCCCCATGT TTGGCAACAT TCTCAGCAAA 
TCGTCCGGCA CACACACGAT GCTTGGACCC GATTTTATCG CTGGGTTGCT CGATCGCTTC 
ATTCAAGTCC ACCCGCCCAA TTTCAACGCG GCAGCAAAGA AGGAGTTGGA GCATAATGTG 
CAGTCTCGAT ACATCCAAGG AAATCCCGAT TCGAACCCGC TGCCTCCCTC TCAAGTGCTG 
TCCGCACTTG ATCTAATGCG ACTACTGGCA GACCGACCCC CGAATGCCTT GACTCTCTAC 
ATCCACAAGA CCGGGCAAGA CTTCACCGAG AATGAAGAGA CTTGCTATAA CTACCTCCGG 
AATCGTCCGG CCAACTGCCA GTTGAGTGAG GAGCAGGTAT CCCACGCGCT CATGTATACG 
ACCATCAGCC AAACCCCTCC GCATAACCCT TCGGTTCTTG TCACGGCTCT CAGGCGTGTT 
CTGCCAGGTA CCTTCAGATG GCAGGATGTG GTCTCGTACT TTGATCACAG CGGTGCCCGA 
ATCACTGCGC CCCAGTTTCT TCGTCTCTAC AATGCTCTGC TGCCGATAGC GCAAGACGAC 
AGCCAGGGTT TTGATATTCA GCGGCTATGG GGTGGCGAGT GGGAAAACCC AGAGACTCAA 
ATGTCCTTTG TCTGCGCATA CACCTCACTG CTGCCTGAAC AGCTGGACGC CACCACCATC 
CCGGGCCTGA AGCCGACCTT CACCTTGGAC GACTATGCAC AAGCACCCCA GATGATCCGG 
GAAACGGCCG CATATGCAGT CAGGCATCCC TTGGTATCCG AAGCGGCCAT GTCTGCCATC 
TTCAACGTCG CCCTCCACTC TACACACGCC TCCAAGTGTA CCGAAGCCAA GCGTCTTTTC 
CAGGATGTCG TCGTTCCCCA AGTGGCCATC TTTGTGGTCT CGGCCTTCAG GGTGCCCAAG 
CCTTGGCCCA AGCTAGCTGA GGACGCTCTG GTTGACTTGT TCATCGAGTC GTTATGGGCA 
GCCAAATCAC CCGAGACCGA TTTCGTGATG AATAGCCTGT GGAGGTGGGA CAAAGACTGG 
GTCAAAATGA GGTTGATAGA TGCCCACGCG CACAGGCCGA TATGCCTGCC ACAAATATTC 
GAGCATTCCA TACGGCAAGG CTGGCTCGAG GAGCTTGTCT ATCTAATCAA TGGCTTTGGG 
TTCGATCTCA CTGCTCTAGC CCACGCCGAG GGCTACCTCG ACCTGCAGGA GTGGGCACAG 
AAGAACGCAG AACGGAGTAC CGAGATGGCC TCGGCCCTTC TTCAGTTCCT GATGATCAAG 
GCGAATCTCG AACTGCAGTA TCAGCGTTCT GGGCCCGGTG CCCCTAAGCA GAGCACCACC 
CTGCAGGTTC GGACAGTGTC TGCCATGCTT CAGATCCTGG AGGAGTTTCT GCCCAAGTCG 
CCGATCCCGG AGCTTATTGT GGTCCAAAGG CAATGTATCA CCGCGTACCC GAGGCTTATC 
AATTACGGCG AAGGGTTCGA TGACATTATT GACGCCAATG GTCGTGATGG GAATTCCTTA 
CCCCAGGCTG CTAATGTTAG AATGGAGGAG CACTACAAGA AAATGTACAG CGACGAAGTC 
GCGGTACGAA CCATCGTTGA GGTTCTCGAC CAGTACAAGC ACTCGCGAGA TCCTCTCGAA 
CAAGACACCT TTGCTTGCAT GATTCACGGC CTGTTCGATG AATACGCGCA TTATGAGAAT 
TATCCTCTGG AGGCGCTCGC CACCACGGCT GTGCTCTTCG GCGGCATCAT CTCTCACAAG 
CTCGTTTCGG AGTTGCCCCT CAAGATCGGA CTTGGCATGA TCCTCGAGGC TGTCCGGGAC 
CATCAACCCC AGGAGCCCAT GTACAAGTTT GGCTTGCAAG CTCTTCTTCA ACTCCTACCT 
CGCTTACGCG AATGGCCAGG GTTTTGCACG CAGCTATTGC TCATCCCGGG TCTTCAGAAT 
ACCGAGGCCC GCAAGAAGGC CGAAGATGTT CTTCGTGAGC ACGAGGAGGA GACGATGCGC 
GCCCGCGGAG GCGCCTTACC GCTTGGCGCG ACCATGGGTA ACGACAACAT GACAAACGGT 
AACCTTGACG AGGCTCACGG TTCGGAGCAG CACCCGCCGC CATTCGCGTC GGTCAACGTT 
GATCCGCCTC CACCGGGGGT GATTTATGAG GACCCAACCG CCGAGGCCCA GGGCAAGATT 
CAGTTCTTCC TCAACAACCT TACTGAAACT ACACTGCAGA CCATGTACAA GGACCTTCGC 
GACATGTTGG AGACCAAGCA TCAGCAGTGG TTCGCTAGCC ACCTGGTTGA AGAACGTGCC 
AAGATGCAGC CGAACTACCA TCAAGTTTAC CTTGACCTGG TAAAGATGTT TGAGGATAAG 
TCACTGTGGG CCGAGGTTTT GAGGGAGACC TATGTCAGCG TCCAGAGAAT GCTCAATTCC 
GACGTTACGC TACAGAACTC AACTGAGAGG ACGCACTTGA AGAATTTGGG CGGCTGGCTC 
GGTCTTTTGA CGCTCGCGAG AAACAAGCCC ATTAAGCATA GAAATATCGC TTTCAAACAG 
TTGCTTATGG AAGCGCATGA TACTAAGCGT CTCCTGATTG TTATACCTTT CGTCTGCAAG 
GTCCTCGCTC AAGGAGCTTC ATCAGAGGTC TTCCAGCCAC CCAATCCCTG GCTGATGGAC 
ATTATCCATC TCCTGATCGA GCTGTACCAC AACGCCGAGC TGAAGCTGAA CTTGAAGTTC 
GAGATTGAGG TCTTGTGCAA GGGCCTCAAG CTGGACCACA AGAGCATTGA GCCGTCTGGC 
GAGATTCTCA ACCGGGTTCC TGTCGAGGAA GTTCCTGATC TGAATGGGCA AGAGCTTGAG 
CCCTACGACA ACACCTCTAT TAACGGTATG GGTAGTGTCG CGGGTGGCCT CCCCGCTCAT 
GCCTCTCTTC CGATCCCCGA CCTTTCCGCC AGCCTCTCGA TCCCGCCAAC CGAAGTCATT 
GATCAAGCCA AACTGCACAG CATTACCTCT CAAGCAGTTA CGCGAGCCCT GCAGGAGATC 
ATCCAGCCAG TGGTTGACAG GTCCGTGACC ATTGCGGCGA TAGCCACACA GCAAATGATC 
CACAAGGACT TTGCCACCGA GCCTGATGAG AACCGGGTGC GGGACGCGGC GATCAACATG 
GTCAAGTCAA CTGCGGGCAG TCTTGCCCTG GTCACGTCCA AGGAACCCCT TCGCTCCAAC 
ATGGCCAACT ACCTTCGTGC TCTCTCGGCT GAGATCGGTC TAGTGCTGCC TGAGGGTATC 
ATCATGCTCT GCATCAACTC GAATTTGGAT CTCGCTTCTA GCGTAATCGA GAAGTCGGCT 
GAAGACCGTG CGGTTCCGGA GATCGAGGAG ATGATTCAAC CTGAAATTGA AGCCAGACGC 
CGGCATCGTG CGCAGCGTCC GAATGAGCCA TACATGGATC CTGGACTCAC TCGCTGGGCC 
TGGACCATTC CTCATCCCTT CAAGTTGAAC CCAAGCATGA CTGGTCTTAA CGCGGAGCAA 
ATGGCTATTT ACGATGACTT TGCCCGACAG CCGAGGGCAG TCGCTTCGAC GACAGGACCG 
TCCCACGCAG CCTCCACATC GGATGCCAGG TCTCTCGCAA ATGAAGTCCT CGGAGATCAG 
TTCAACACCA TTTCGGCCCT TCCCACACCT GCGGAGACAC CTTCGCTTCC TCACCTCGGT 
GGTCAGCTTC AGCACTACCC CCAGGGAAGT GGCGGATTGG CAAACGGACG TCAAGCCGGG 
CTTAACCAGG TGGATGCCAG GACCATTGCT GACAGAGTCA ACAAGCTTTT GGAGTCACTG 
ACCGCGGCCG CCACTGGTGC CACCGAGGAA CACTTCAATG AACTGCCCCG CGCTCACCAA 
GTCCTGGACA TCATTGACGC TCTTGTGCAA CTCATTATCA AGACTCAGCA GACGTCTGAA 
GAGTTTGCAG CATATGCCGC AACCCAGATC GCCCAGTTGA TCTTCAGACA GCCAGGCGAT 
ACCCTCTTGC TTGAAAGTCT CGTCCATGTC CTCGAAACCC TCCGTAAGAT TGCCGGTCCG 
GCCACGAGCC AGCAGATCAG GAGTCTCTTC CATCAACAAC CAGCGGAATA CTTCCTCAAC 
CTTTCGCTCA TTGCAGCACT GCTCGGCACG GACCTGCTCG ACTGGAGAAG CATTGATGCA 
GCCATGTCTA TGGCCCTCCA GCAACGCAAA GAGGGATCCG TCGAGTTCCT CGAGCAGCTT 
GTGGATCTTA CGCTGCTGAG TGAGAGCCCT CTTGCGCTCT ATGCTGACTT TGTCCGCAGT 
TTGGAGGAAG CATGGGCCTG GATCAGTGAG AATCCGGATG TTCCAGGAGG CCAGAGTCTC 
AAGTCCAAGA TCCTTGCCCC TCCTCCGGAC TTGCCTGAAG GCCTCACTCC GGCAGAGATC 
CTCGCCATCC GCCAGGACCA GATGGAGTAT GTCTTTGACG AATGGGTTCA CCTTTGCAAC 
AACCCTGCCG CCTCCTCCTC CAAGTCATCG ACGATCTTTG TACAGCAGAT GCGCTCCAGA 
GGAGTCATTG CTAGCACTGA TGATTTCCTC ATGTTTGCAC GCCTAGCCAT TGACAAGTCT 
GTGGACCGCT TCGAGCATGT TGTTCACCTT AATGGAACGA TAACGGAGGC GTACCAGGCG 
ATTGATGCTT TAGGCAAAAT GATCCTCATC TTCTTCCAGT CCCACGAAGA TAATGCCGAC 
GGACAAACGG CTCGTGCTGC CTTCATTGAT TCCATCCTCG CCCTTGGGGT GCTGGTATTG 
AATAGCCACC ACGTCAAGCG TGGCGACGCT TTCAACTCAC GGGTGTTTGT TCGGTTCTTC 
TCCTACCTCC TTCACGGCAT TCACTCTCTG GAGGAGCTTC CAGAAAATGA GCGTGATCGC 
TTCATGCTTA GCTTTGCCGA GCGTCTTTAC GACCTGCGGC CGAGCCTCTA CCCAGGCTTC 
GTCCTTGGCT GGCTCACACT TGTCACCCAC CGTGTCTTCC AGCCAGCCAT GCTTCAAATG 
GCTAACAACG CGGGCTGGGA GGCGTACACC AAGATCCTTC GGGATTTGCT CTCTTACCAG 
GGCGAGCTGC TGAAGCCGCT CGAGCTCTCG ATCTTCGCCA AGGAAATGTA CCAGGCCGTC 
CTAAAGCTCC TCATCATCCT GGGGCATGAT TTCCCCGATT ACGTGTCTGC CAACCATGTG 
CTGCTGTGCC AGAGCTTGCC GCCTCACGCA ACGCAATTAA GGAACATGAT CTTACTGGCT 
ACTCCTGCTT CGGCCGCGAA GCCGACCGAT GCCTTCCAGC CCGGCTTGAA ATTGGATCAG 
ATCGCAGATA TCAAAGAAGC AGCGATCAGT GTCTACGATC CTGCCGAGCA GCTACGCCAA 
CTGGGGCTGC TTGACATTCT GGATCAGGCT TTGCAGAACG GCCCGTCCGA AGATGCCATT 
GCGCAGATTA CACACGCTAT CACTAGGACT GGTAGCGGAA CGACGGACTA TGGCTTTGTT 
CCCATTGACG TCAACCGTCA AGTCATCGAC GCCGTAATCA CGCATATTGG CAACTTTGCC 
GTCAACCGCG CCGCCACCAG GAACGACGCC TCTGTGTTCG TCCCCGGCGC ACCAGACCTC 
AAGACACTGC AAATGCTCAT CACGGAACTC TCCCCCGATA CGAGGTACTT CGTTCTGAAC 
AGCATGGCGA ACTGGCTCCG CTTCACCAGC GCCCACACGC ACTATTTCAT TCAAGTCTTC 
CTTGACTTCT TTGGCCACGA CGTCGCCTCC ACGGATCCCG AGGAGATGGA CATTCGGCAG 
CAGATTGTTC GGATCCTCCT TGAACGGTTA ATAGGCTACT GGCCGTACCC GTGGGGTCTG 
ATCATCACCA CCATGGAGCT AGTCAAGAAC GAGAAGTACA ACTTCTTTGA GCTGCCTTTC 
ATCAAGGCCA CCCCTGAGGT GCGGAGAGTG TTTGAGGATA AGGTTCGCCA GCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU04766 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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