not1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	not1
Entrez Gene ID	2542001
Full Name	CCR4-Not complex subunit Not1 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018754.2	NP_593323.1	putative CCR4-Not complex subunit Not1

Pathways from BioSystems

Homologies

Neurospora crassa	NCU04766	XP_960299.1
Schizosaccharomyces pombe (fission yeast)	not1	NP_593323.1
Magnaporthe oryzae (rice blast fungus)	MGG_02476	XP_003709270.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following not1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the not1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102183	NM_001018754.2 Latest version!	Schizosaccharomyces pombe putative CCR4-Not complex subunit Not1 (not1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

not1 ( NM_001018754.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593323.1 Schizosaccharomyces pombe putative CCR4-Not complex subunit Not1 (not1), mRNA.

Price & Availability↑

CloneID	OSc102183
Clone ID Related Accession (Same CDS sequence)	NM_001018754.2
Accession Version	NM_001018754.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative CCR4-Not complex subunit Not1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018754.1.

ATGAAGAGCC AAGAACAACA AACGCCGGAG ACACGAGAGA AATCTAGTTC AACTTCTGAA 
TCTGGGCATC CTATATCACT AGATTCTAAG ACCCCTCCAC CAAACGATTC AAAAATTGAA 
AGTATTGTTA AAGCACAAAT CCTTTTCTTA TTGTCGACCT TACGGGAAGA CCAATATGAT 
ACGAAACTTG AACAGATTCG TCAACTTATC AACAAAAATG CACCCAGAGT ATACCACCAT 
TTTCTTCGCC GTCTAATTCA AGGAAATTCA TACCGTATTT TTGGTACCGG TAAATCTTCT 
GATGGACTAG CTACTTATAA ATTACTACTA GATGAATTAA AATCTCTCAC GAAGTCTTGG 
GTCATGGCGA AACGTTTTAG TGATGCTATT TCAGGTTCTG ATTCTGAAGT ATTTGAAGAT 
TTCGATATAG AGGCATTTTT AGACCATTTC CAATTCTCCC CGTTAGAAAG GACTAACCTT 
TTAATTGGAC TTACCACTTC TGTTAAACCT ACTTTAGCAA AGAAAGCTTC TGCTTTGCTT 
AAAAACACAT TCCAATCTCT TTTAAACCAA CTATCCGATC CTACTCAGCA ACAAAGTATT 
GATAAAAACG ATTTGGATTT GCTCAGGGAA GGTTTGTTTA CCACCGACTA TGACAGAATA 
CCCTTGATTA AAGTTCCAAA GTTTGATGCT ATAATTGAGA AAAAGTTGGC GAATTCACGC 
CCTCTTACAG CTTTGTTTGG TCCGATGAAA GCTTCCAAAG CTACACTTAA GTCTATGAAA 
GAAGCAATTC TTAAAAAATA TCCCCCGGGA ACGGCTACTG AGACTGATGT TGCTTATCTT 
ATAAATTCAC TTGTAGCTAT ACATGATTAT GGTGCTTGGG ACACTGTTTT GATGGGTAAA 
GCTATCGTTT CCTCTTTTGA AAACCTAAAT TGGGAAGCTA TGCTTTCTAT GTTCGATAAT 
CCTAAATTTA TGATAACGGG TACACCTAGT CTTGTACTGT TCTTCACAAT CTTTACAAAT 
GCTTACAAGT TAAGGCAAAC TAATTTTACG TTGGACTTTC TCTGGGTTCT TTGGAGAAAT 
CCTTTACCGC AACTTTCCAT CATTTCTCAT ATTATCCTAT CCCCAACATC GATGTTTGAT 
ATAAGAGAGT TCAATGTCAA TCCTGTAGTT TCTGTTGATA GCTTAAAGGA TTATAGCGAA 
GAGCTTGTTA ATTATGCGAA AATATATGAA AAAAGTAATT TAAATTGCAT TGAACTTGTC 
CAAATCCTTT TACGCCTGTT ATCTGAGGTA GTCACCTACG AAACTTCACT GTTCTTGAAT 
TTTTTGGATG AGAAAGTTTC TGCCGAGCTT TTGCTTTTGG GTACGCTGCA GCTCCCTTTG 
GCATGGAATC CTGTTCAAGA GTCATTAGCG TTTCAATGGT GTTCAGACTT TTTTTCCAAT 
TTCAAAGAAC ATAAATTTGT CTTCGTTAGG ATTGAACGAA CAAACCCTCA ATTTTTGTTC 
GCCTTTTTAC GCAGTTTGTG GCTGAAGGAT AGTTCATCTG TGAATCAGGT AGTAGAGTTT 
ATTATCGAAA ATGATTTTAC ATCTCACATC GGCAACATTC AACCAAATCG ATTTGCTTTA 
GAGATTGCTG CACTTGCAGC TGCTCGCAAA GCGTTATCGT TTCAGGATTG GCTTGACAAA 
AAGATGCTCG AATTTGAGGA TGCAGACGGA CTTAATGTTT TCTTGGTAGA GGTTTTGGAT 
TTTTTGATGA GTCGAGCGGC ATTACAAAAA AATGAGTCTG AACAAAAGGA AGAGTCTGTT 
GTTCTTTCCA TCGATACGGT TAATGTTCTA TTGACAACGT TGATGGACAA CGTTAGTATT 
TCGGAGGAAG TCAGTGAACA TATAAAAGAT GTCCAAATTT TGTGTCTTCA AGTTTATCCG 
CGTCTCTTCA GTTTGGGTCA TGATCGGGAT AGCATTGTTA TTGCTACTAA CACGACTAAT 
TCTTTTTCAC CGGATGTAGA ATCGGAGGTA GAATCGTACT TTCAAGCTCT GTATGAACGA 
CGCATATCAA TTGGGAAGAT TGTTTTGACG CTTCAAAATT TTAAAAAATC GGAAAACCCG 
CGTGACCTTG ATTTGTTTGC ATGTTTACAG CATAGTCTTT TTGATGAATA CAGGTTTTTC 
CCTGACTATC CTTTGGAAGC GTTAGCTTTG ACAGCAGTCT TGTTTGGAAG TTTGATTCAA 
TTTGAGCTTC TATCTTTTGT TCCCTTAGGT GTTGCTTTGC GTTACGTTTA TCAGGCGCTG 
CTTATGCCCA CCGACTCCAA AATGTTTAGA TTTGGTATGC AAGCCTTGGT TCAGTTTCAA 
GAAAAGCTAC CAAAATATAT TAACTACTGT AATCTTATAT TGGGTATACC TTCTCTTCAG 
CTAGTGCGCC CTGATATATA TGACTCTATC AGAGAAATGA TTGCGTCTAA TGAAAATACT 
GAAGTTGAAA AAGATAAACT CGATAGTTTT TCTGCTCTCG CTAATCCTGC AAGTCCAAAA 
GCGCCTGAGT ATACATTCGT TTCTATCCAT GTTCCTCCTT TAAATGTGCC AAATGCTGAA 
GGTGAAATTG AAGAGAGCGT TTGTGATAAC ATCTTATTTG CAATTAACAA TCTTTCTCAG 
CTTAATTTTA ATGAAAAGTT AAAAGACGTT AAGGATAATT TGACTCCCGC ATATTTGCCT 
TGGTTTTCGC ATTATATTGT TACACAACGT GTTAGTCGTG AAGCCAATTT CTTGTCTCTG 
TATGGAAAGT TTTTAGAGGA GTTAAAATCA AGTGATCTCT ACAAAATTGT TTTTCGGGAT 
ACTCTTCAAG CAATATTGGA TATAATGAAC AATAACGCAG AGACATTATC ACCTTCAGAG 
AAAACAAACC TTAAGAACCT AGGTTCGTGG TTAGGAAGTA TTACTCTATT GCGCAACAAG 
CCAATTACAA CCCTTCAAGT TTCTTTTAAA GATTTACTAC TTGAAGGGAT TGATAGTGGT 
CGAATTGATA GAGTTTTACC ATTTGTGTGC AAAGTGCTTG AGAAAGCTTC TTCATCTGTC 
ATATTTAAAC CTCCTAACCC TTGGTTGATG GGAATTTTGA AGTTACTGGT TGAGTTGTAT 
CAGTTTGCTG ATTTTAGGCT TAACTTAAAA TTTGAAATTG AGTTATTACT TAATAACTTG 
AATGTCAAGA TGGACAACAT TGAACCTTCT GAAATGTATC GTAACCATCT TGTCCAAAAA 
GCCGATTTAG AAAAGGAGCT TCCTGAGGAC GTACTTAACG CTGAGTTCCC TGATGGCACC 
GATGTTATAA CCCAATATAT AGTAGCTGCA TCTTCGCAAA TAACAGTAAC GGATGCGGTA 
GCTCAGGTGT TTGGAAAACC CAAACCTGCT ATAAAAAATA TTACTCAATT AATAATTCAG 
CAATCTGTTT TAGAAATAAT TTCAGCAGTA GTGCGACGAT CTGTTGGGAT TGCAGCTATT 
ACTACCAAGA GCCTTTTACA GAAGGATTTT GCAGCTGAAT CAAATCCTTC CCGACTTTTA 
TTAGCCGCTA GACAAATGGC TAAAACCCTT GCCGGAAATT TAGCAATGGT TACTTGTCGT 
GAACCTTTAC AAATGCTTAT GATCAATAAT TTTAGGACGA TAGCTTTACA GGACGTTGAA 
AACGTTCATG CTGCTGTGGC ACAAGCAATT GATGAGCTTG TTAGTCAAAA CTTGTTCGTC 
GCTAGCTCTG TAATAGAGAG TGTGGCATCG GAGACGGCTA TTGCTGAAAT TGACGCGGAA 
ATTGAGCACA TGATAGTGGA GCGTGTTCGA CACCGTAAGA CAACACCAAA TCTTCCATTT 
GTTGATCCAG CTGGAGCGGC TAATTTACAC TTGAACCTTC CTAGCGTATT GAAATTATCG 
TCGGAGCTTA CGCCTCAACA ATTTCAATTA TATGAAAATT TTGACCGCTT GAGCCTATCT 
ACCATAATGA GTAACAATTC TTTTACCAGT CTTAATGGAT TACGTACCGA CAGTGCCGAC 
AGTACGGACG CTTTGAACTC CAATCTTAAT AATACAGTCG AGAATGAAGC TAATCAGACA 
GCATTGAACT ACGCTAGAAA TTTGCTCATA ATTATTGGGC AGTTATTTCA GCTGGCTGCC 
CAAATGCCTT ATACCAGTAT GCAGGAGGTT CCAGCTGATC ATGAATTACA TGAACTCGTT 
AATCAATTTT TAACTGGCGT TGCCTCTATT AATCATCCCA TTGCAGATCA CGTATTTTTG 
TTATGCGCTC AAGAATGTTG TCGCATACTT CTCACCGACA GTAAATCACC TTTCATTTTG 
GAAGTGTTTT CTGCAATTTT AGAGTATATT TGTCAAGCAT CCACAAAAAC TGCGATAAAT 
GTCTCTTTGT ATTGGAACTT CTCTAATGAT CTTGAAAAGC TTAATCTTCC TATTATTTTG 
AGTTTAATTA GATTTGGAAT TCTCACGTCT GGTGAAGTTG ATTATCACGT AGCTAGAGGT 
GTCCGGTCTG AACAAGGATC TGGTCCTGTT ACCGATTTTG CAATAGAGTT ATTGAGGACA 
GCGGTAGGTG GTGAGAATCC AATGGCTTTA CCTGGTAATT TTGTGAATTC TATCACTTCA 
CTCTACGAGA TTAGTGAATC TTTCTCTGGT GAGACGAAAC AGGCCTATGA GCAACTTGTT 
GAAACAATGA ACAAGAGTGT ATCACCTGCA TCTGAAATTT TGTCTGATCT CGATAATAAA 
CAACAACTTA ACGATCAAAT TATAATCGTT TTTGTCTCTT GGGTTCATCT GTTGCGCAAT 
TCGGCAACCA ACGATGAAAC TAAAGCTGCA TTTGTATACC AATTGCATAA ACAAGGAATT 
CTGTCAGAGC CTGAGTTATG TATTCAGTTT TTCAGATGTA ATTTAGAGGC AGTTTTAGTT 
GCCTTTTTGG AAGCAGCTTC CGTTAATGCC CCCGATTATT TTAATGTTGA CGCTTATGCC 
TCTCTATTGG TAAACGTTGT TAAGTACACT GAAGGTAGTA CTGAAGGAAC GGTATCTTCA 
AAAAGCGTGC TTTTTAGAAA GATCATTGCT TTGATAATCG GTGTGTTTGC CGAATTGCAT 
AATTCCATGG CTGAATTTGT CCATCAAAAA ACATTTTTCA GATTGTTCTC TAGTATACTC 
TCCGAGCTTG ATGATGCTAA GGATGTATTG GAGTCCTGCT TCGTCGATAT CTATAGTGTT 
ATTTTAGAAT GCTTCCTTGC TATTCAGCCA CGCTCTTTCC CAGCGTTTAC ATTTGCCTGG 
CTTAGCCTTA TTTCACATTC ATACTTACTA CCGAAGGTTC TTCTTGTCAA TAATGATAAA 
ATTAATGATT TATTTAGTGA GATCCTGATG TCCTTTTTAA AATTTTTAGA TTTGAAGGAT 
GATATTGATA AAGCCCAATT TAAACTTCTT TATAATGGCT TCTTGAGGAT CATACTTGTT 
CTTCTTCATG ATTTCCCTGG CTTTTTGGCT ACCCATTGTT ACCAATTGAT ACCTTATATT 
CCATTAGAAT GCGTGCAACT TCGTAATATG GTATTGAGCG CGTTTCCTAG TGATCTTCAT 
CTTCCTGATC CATTTGCTCA AGGTTTGAAG GTAGGCAGAT TACCTGAGGT AACCCGCGCA 
CCATTAATTT CAAATTCAGT TTCTGCTTCA CTTGAGAAAT TTGCGTCAGC GTTGGATTTA 
GAAGCTTGTT TCTCTTCTTC CAAACCAGCG GAAGTTGCCA AGCTATTGTT AGAAGTGTAC 
TCAAGTCAAG ATACCCCTAC TAAATTAAAC GAATGGGCAA ACTATTTTAT GCTTTGTTTA 
ATACGCCACG CTACTCGTGA TTCCCCTGCC AAGCAAGCCC CACAATTTCA ATCAAAATCT 
CCTGAATGTG TAATTATTAG TACTATGAAT CGCAGTTGCG ATTCAAAGTG CCGCTATTTC 
TTGTTAACTG CGATTGCTAA TCAGCTGCGT TACCCTAGCA GCCATACTTA CTATGCTAGC 
TGTTGTTTCT TGTACCTTTT CAAGAGTTCT TCTAACAACC CTCAAGAATT GCTTATCAAA 
GAACAGATGA CAACTGTACT TTTGGAACGC ATAATTTGCA ATCGACCACA TCCTTGGGGC 
CTTTTAATTA CATTTACTGA ATTATTAAAA AATGAAGACT ATAACTTTTG GAAACACCCT 
TATATAAAGC GAAACGATGA AATCTGTCGT TTGTTTGATA GTCTCCATGA ACATGTCATG 
GCACCCTCCT CAGCTAATAA TGTCTCTACC GAAGAGGCTA CAGAACTGAT GGGTCAAGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593323.1
CDS	169..6471
Translation	MKSQEQQTPETREKSSSTSESGHPISLDSKTPPPNDSKIESIVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRVYHHFLRRLIQGNSYRIFGTGKSSDGLATYKLLLDELKSLTKSWVMAKRFSDAISGSDSEVFEDFDIEAFLDHFQFSPLERTNLLIGLTTSVKPTLAKKASALLKNTFQSLLNQLSDPTQQQSIDKNDLDLLREGLFTTDYDRIPLIKVPKFDAIIEKKLANSRPLTALFGPMKASKATLKSMKEAILKKYPPGTATETDVAYLINSLVAIHDYGAWDTVLMGKAIVSSFENLNWEAMLSMFDNPKFMITGTPSLVLFFTIFTNAYKLRQTNFTLDFLWVLWRNPLPQLSIISHIILSPTSMFDIREFNVNPVVSVDSLKDYSEELVNYAKIYEKSNLNCIELVQILLRLLSEVVTYETSLFLNFLDEKVSAELLLLGTLQLPLAWNPVQESLAFQWCSDFFSNFKEHKFVFVRIERTNPQFLFAFLRSLWLKDSSSVNQVVEFIIENDFTSHIGNIQPNRFALEIAALAAARKALSFQDWLDKKMLEFEDADGLNVFLVEVLDFLMSRAALQKNESEQKEESVVLSIDTVNVLLTTLMDNVSISEEVSEHIKDVQILCLQVYPRLFSLGHDRDSIVIATNTTNSFSPDVESEVESYFQALYERRISIGKIVLTLQNFKKSENPRDLDLFACLQHSLFDEYRFFPDYPLEALALTAVLFGSLIQFELLSFVPLGVALRYVYQALLMPTDSKMFRFGMQALVQFQEKLPKYINYCNLILGIPSLQLVRPDIYDSIREMIASNENTEVEKDKLDSFSALANPASPKAPEYTFVSIHVPPLNVPNAEGEIEESVCDNILFAINNLSQLNFNEKLKDVKDNLTPAYLPWFSHYIVTQRVSREANFLSLYGKFLEELKSSDLYKIVFRDTLQAILDIMNNNAETLSPSEKTNLKNLGSWLGSITLLRNKPITTLQVSFKDLLLEGIDSGRIDRVLPFVCKVLEKASSSVIFKPPNPWLMGILKLLVELYQFADFRLNLKFEIELLLNNLNVKMDNIEPSEMYRNHLVQKADLEKELPEDVLNAEFPDGTDVITQYIVAASSQITVTDAVAQVFGKPKPAIKNITQLIIQQSVLEIISAVVRRSVGIAAITTKSLLQKDFAAESNPSRLLLAARQMAKTLAGNLAMVTCREPLQMLMINNFRTIALQDVENVHAAVAQAIDELVSQNLFVASSVIESVASETAIAEIDAEIEHMIVERVRHRKTTPNLPFVDPAGAANLHLNLPSVLKLSSELTPQQFQLYENFDRLSLSTIMSNNSFTSLNGLRTDSADSTDALNSNLNNTVENEANQTALNYARNLLIIIGQLFQLAAQMPYTSMQEVPADHELHELVNQFLTGVASINHPIADHVFLLCAQECCRILLTDSKSPFILEVFSAILEYICQASTKTAINVSLYWNFSNDLEKLNLPIILSLIRFGILTSGEVDYHVARGVRSEQGSGPVTDFAIELLRTAVGGENPMALPGNFVNSITSLYEISESFSGETKQAYEQLVETMNKSVSPASEILSDLDNKQQLNDQIIIVFVSWVHLLRNSATNDETKAAFVYQLHKQGILSEPELCIQFFRCNLEAVLVAFLEAASVNAPDYFNVDAYASLLVNVVKYTEGSTEGTVSSKSVLFRKIIALIIGVFAELHNSMAEFVHQKTFFRLFSSILSELDDAKDVLESCFVDIYSVILECFLAIQPRSFPAFTFAWLSLISHSYLLPKVLLVNNDKINDLFSEILMSFLKFLDLKDDIDKAQFKLLYNGFLRIILVLLHDFPGFLATHCYQLIPYIPLECVQLRNMVLSAFPSDLHLPDPFAQGLKVGRLPEVTRAPLISNSVSASLEKFASALDLEACFSSSKPAEVAKLLLEVYSSQDTPTKLNEWANYFMLCLIRHATRDSPAKQAPQFQSKSPECVIISTMNRSCDSKCRYFLLTAIANQLRYPSSHTYYASCCFLYLFKSSSNNPQELLIKEQMTTVLLERIICNRPHPWGLLITFTELLKNEDYNFWKHPYIKRNDEICRLFDSLHEHVMAPSSANNVSTEEATELMGQV

Target ORF information:

RefSeq Version	NM_001018754.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative CCR4-Not complex subunit Not1 (not1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018754.2

ORF Insert Sequence:

ATGAAGAGCC AAGAACAACA AACGCCGGAG ACACGAGAGA AATCTAGTTC AACTTCTGAA 
TCTGGGCATC CTATATCACT AGATTCTAAG ACCCCTCCAC CAAACGATTC AAAAATTGAA 
AGTATTGTTA AAGCACAAAT CCTTTTCTTA TTGTCGACCT TACGGGAAGA CCAATATGAT 
ACGAAACTTG AACAGATTCG TCAACTTATC AACAAAAATG CACCCAGAGT ATACCACCAT 
TTTCTTCGCC GTCTAATTCA AGGAAATTCA TACCGTATTT TTGGTACCGG TAAATCTTCT 
GATGGACTAG CTACTTATAA ATTACTACTA GATGAATTAA AATCTCTCAC GAAGTCTTGG 
GTCATGGCGA AACGTTTTAG TGATGCTATT TCAGGTTCTG ATTCTGAAGT ATTTGAAGAT 
TTCGATATAG AGGCATTTTT AGACCATTTC CAATTCTCCC CGTTAGAAAG GACTAACCTT 
TTAATTGGAC TTACCACTTC TGTTAAACCT ACTTTAGCAA AGAAAGCTTC TGCTTTGCTT 
AAAAACACAT TCCAATCTCT TTTAAACCAA CTATCCGATC CTACTCAGCA ACAAAGTATT 
GATAAAAACG ATTTGGATTT GCTCAGGGAA GGTTTGTTTA CCACCGACTA TGACAGAATA 
CCCTTGATTA AAGTTCCAAA GTTTGATGCT ATAATTGAGA AAAAGTTGGC GAATTCACGC 
CCTCTTACAG CTTTGTTTGG TCCGATGAAA GCTTCCAAAG CTACACTTAA GTCTATGAAA 
GAAGCAATTC TTAAAAAATA TCCCCCGGGA ACGGCTACTG AGACTGATGT TGCTTATCTT 
ATAAATTCAC TTGTAGCTAT ACATGATTAT GGTGCTTGGG ACACTGTTTT GATGGGTAAA 
GCTATCGTTT CCTCTTTTGA AAACCTAAAT TGGGAAGCTA TGCTTTCTAT GTTCGATAAT 
CCTAAATTTA TGATAACGGG TACACCTAGT CTTGTACTGT TCTTCACAAT CTTTACAAAT 
GCTTACAAGT TAAGGCAAAC TAATTTTACG TTGGACTTTC TCTGGGTTCT TTGGAGAAAT 
CCTTTACCGC AACTTTCCAT CATTTCTCAT ATTATCCTAT CCCCAACATC GATGTTTGAT 
ATAAGAGAGT TCAATGTCAA TCCTGTAGTT TCTGTTGATA GCTTAAAGGA TTATAGCGAA 
GAGCTTGTTA ATTATGCGAA AATATATGAA AAAAGTAATT TAAATTGCAT TGAACTTGTC 
CAAATCCTTT TACGCCTGTT ATCTGAGGTA GTCACCTACG AAACTTCACT GTTCTTGAAT 
TTTTTGGATG AGAAAGTTTC TGCCGAGCTT TTGCTTTTGG GTACGCTGCA GCTCCCTTTG 
GCATGGAATC CTGTTCAAGA GTCATTAGCG TTTCAATGGT GTTCAGACTT TTTTTCCAAT 
TTCAAAGAAC ATAAATTTGT CTTCGTTAGG ATTGAACGAA CAAACCCTCA ATTTTTGTTC 
GCCTTTTTAC GCAGTTTGTG GCTGAAGGAT AGTTCATCTG TGAATCAGGT AGTAGAGTTT 
ATTATCGAAA ATGATTTTAC ATCTCACATC GGCAACATTC AACCAAATCG ATTTGCTTTA 
GAGATTGCTG CACTTGCAGC TGCTCGCAAA GCGTTATCGT TTCAGGATTG GCTTGACAAA 
AAGATGCTCG AATTTGAGGA TGCAGACGGA CTTAATGTTT TCTTGGTAGA GGTTTTGGAT 
TTTTTGATGA GTCGAGCGGC ATTACAAAAA AATGAGTCTG AACAAAAGGA AGAGTCTGTT 
GTTCTTTCCA TCGATACGGT TAATGTTCTA TTGACAACGT TGATGGACAA CGTTAGTATT 
TCGGAGGAAG TCAGTGAACA TATAAAAGAT GTCCAAATTT TGTGTCTTCA AGTTTATCCG 
CGTCTCTTCA GTTTGGGTCA TGATCGGGAT AGCATTGTTA TTGCTACTAA CACGACTAAT 
TCTTTTTCAC CGGATGTAGA ATCGGAGGTA GAATCGTACT TTCAAGCTCT GTATGAACGA 
CGCATATCAA TTGGGAAGAT TGTTTTGACG CTTCAAAATT TTAAAAAATC GGAAAACCCG 
CGTGACCTTG ATTTGTTTGC ATGTTTACAG CATAGTCTTT TTGATGAATA CAGGTTTTTC 
CCTGACTATC CTTTGGAAGC GTTAGCTTTG ACAGCAGTCT TGTTTGGAAG TTTGATTCAA 
TTTGAGCTTC TATCTTTTGT TCCCTTAGGT GTTGCTTTGC GTTACGTTTA TCAGGCGCTG 
CTTATGCCCA CCGACTCCAA AATGTTTAGA TTTGGTATGC AAGCCTTGGT TCAGTTTCAA 
GAAAAGCTAC CAAAATATAT TAACTACTGT AATCTTATAT TGGGTATACC TTCTCTTCAG 
CTAGTGCGCC CTGATATATA TGACTCTATC AGAGAAATGA TTGCGTCTAA TGAAAATACT 
GAAGTTGAAA AAGATAAACT CGATAGTTTT TCTGCTCTCG CTAATCCTGC AAGTCCAAAA 
GCGCCTGAGT ATACATTCGT TTCTATCCAT GTTCCTCCTT TAAATGTGCC AAATGCTGAA 
GGTGAAATTG AAGAGAGCGT TTGTGATAAC ATCTTATTTG CAATTAACAA TCTTTCTCAG 
CTTAATTTTA ATGAAAAGTT AAAAGACGTT AAGGATAATT TGACTCCCGC ATATTTGCCT 
TGGTTTTCGC ATTATATTGT TACACAACGT GTTAGTCGTG AAGCCAATTT CTTGTCTCTG 
TATGGAAAGT TTTTAGAGGA GTTAAAATCA AGTGATCTCT ACAAAATTGT TTTTCGGGAT 
ACTCTTCAAG CAATATTGGA TATAATGAAC AATAACGCAG AGACATTATC ACCTTCAGAG 
AAAACAAACC TTAAGAACCT AGGTTCGTGG TTAGGAAGTA TTACTCTATT GCGCAACAAG 
CCAATTACAA CCCTTCAAGT TTCTTTTAAA GATTTACTAC TTGAAGGGAT TGATAGTGGT 
CGAATTGATA GAGTTTTACC ATTTGTGTGC AAAGTGCTTG AGAAAGCTTC TTCATCTGTC 
ATATTTAAAC CTCCTAACCC TTGGTTGATG GGAATTTTGA AGTTACTGGT TGAGTTGTAT 
CAGTTTGCTG ATTTTAGGCT TAACTTAAAA TTTGAAATTG AGTTATTACT TAATAACTTG 
AATGTCAAGA TGGACAACAT TGAACCTTCT GAAATGTATC GTAACCATCT TGTCCAAAAA 
GCCGATTTAG AAAAGGAGCT TCCTGAGGAC GTACTTAACG CTGAGTTCCC TGATGGCACC 
GATGTTATAA CCCAATATAT AGTAGCTGCA TCTTCGCAAA TAACAGTAAC GGATGCGGTA 
GCTCAGGTGT TTGGAAAACC CAAACCTGCT ATAAAAAATA TTACTCAATT AATAATTCAG 
CAATCTGTTT TAGAAATAAT TTCAGCAGTA GTGCGACGAT CTGTTGGGAT TGCAGCTATT 
ACTACCAAGA GCCTTTTACA GAAGGATTTT GCAGCTGAAT CAAATCCTTC CCGACTTTTA 
TTAGCCGCTA GACAAATGGC TAAAACCCTT GCCGGAAATT TAGCAATGGT TACTTGTCGT 
GAACCTTTAC AAATGCTTAT GATCAATAAT TTTAGGACGA TAGCTTTACA GGACGTTGAA 
AACGTTCATG CTGCTGTGGC ACAAGCAATT GATGAGCTTG TTAGTCAAAA CTTGTTCGTC 
GCTAGCTCTG TAATAGAGAG TGTGGCATCG GAGACGGCTA TTGCTGAAAT TGACGCGGAA 
ATTGAGCACA TGATAGTGGA GCGTGTTCGA CACCGTAAGA CAACACCAAA TCTTCCATTT 
GTTGATCCAG CTGGAGCGGC TAATTTACAC TTGAACCTTC CTAGCGTATT GAAATTATCG 
TCGGAGCTTA CGCCTCAACA ATTTCAATTA TATGAAAATT TTGACCGCTT GAGCCTATCT 
ACCATAATGA GTAACAATTC TTTTACCAGT CTTAATGGAT TACGTACCGA CAGTGCCGAC 
AGTACGGACG CTTTGAACTC CAATCTTAAT AATACAGTCG AGAATGAAGC TAATCAGACA 
GCATTGAACT ACGCTAGAAA TTTGCTCATA ATTATTGGGC AGTTATTTCA GCTGGCTGCC 
CAAATGCCTT ATACCAGTAT GCAGGAGGTT CCAGCTGATC ATGAATTACA TGAACTCGTT 
AATCAATTTT TAACTGGCGT TGCCTCTATT AATCATCCCA TTGCAGATCA CGTATTTTTG 
TTATGCGCTC AAGAATGTTG TCGCATACTT CTCACCGACA GTAAATCACC TTTCATTTTG 
GAAGTGTTTT CTGCAATTTT AGAGTATATT TGTCAAGCAT CCACAAAAAC TGCGATAAAT 
GTCTCTTTGT ATTGGAACTT CTCTAATGAT CTTGAAAAGC TTAATCTTCC TATTATTTTG 
AGTTTAATTA GATTTGGAAT TCTCACGTCT GGTGAAGTTG ATTATCACGT AGCTAGAGGT 
GTCCGGTCTG AACAAGGATC TGGTCCTGTT ACCGATTTTG CAATAGAGTT ATTGAGGACA 
GCGGTAGGTG GTGAGAATCC AATGGCTTTA CCTGGTAATT TTGTGAATTC TATCACTTCA 
CTCTACGAGA TTAGTGAATC TTTCTCTGGT GAGACGAAAC AGGCCTATGA GCAACTTGTT 
GAAACAATGA ACAAGAGTGT ATCACCTGCA TCTGAAATTT TGTCTGATCT CGATAATAAA 
CAACAACTTA ACGATCAAAT TATAATCGTT TTTGTCTCTT GGGTTCATCT GTTGCGCAAT 
TCGGCAACCA ACGATGAAAC TAAAGCTGCA TTTGTATACC AATTGCATAA ACAAGGAATT 
CTGTCAGAGC CTGAGTTATG TATTCAGTTT TTCAGATGTA ATTTAGAGGC AGTTTTAGTT 
GCCTTTTTGG AAGCAGCTTC CGTTAATGCC CCCGATTATT TTAATGTTGA CGCTTATGCC 
TCTCTATTGG TAAACGTTGT TAAGTACACT GAAGGTAGTA CTGAAGGAAC GGTATCTTCA 
AAAAGCGTGC TTTTTAGAAA GATCATTGCT TTGATAATCG GTGTGTTTGC CGAATTGCAT 
AATTCCATGG CTGAATTTGT CCATCAAAAA ACATTTTTCA GATTGTTCTC TAGTATACTC 
TCCGAGCTTG ATGATGCTAA GGATGTATTG GAGTCCTGCT TCGTCGATAT CTATAGTGTT 
ATTTTAGAAT GCTTCCTTGC TATTCAGCCA CGCTCTTTCC CAGCGTTTAC ATTTGCCTGG 
CTTAGCCTTA TTTCACATTC ATACTTACTA CCGAAGGTTC TTCTTGTCAA TAATGATAAA 
ATTAATGATT TATTTAGTGA GATCCTGATG TCCTTTTTAA AATTTTTAGA TTTGAAGGAT 
GATATTGATA AAGCCCAATT TAAACTTCTT TATAATGGCT TCTTGAGGAT CATACTTGTT 
CTTCTTCATG ATTTCCCTGG CTTTTTGGCT ACCCATTGTT ACCAATTGAT ACCTTATATT 
CCATTAGAAT GCGTGCAACT TCGTAATATG GTATTGAGCG CGTTTCCTAG TGATCTTCAT 
CTTCCTGATC CATTTGCTCA AGGTTTGAAG GTAGGCAGAT TACCTGAGGT AACCCGCGCA 
CCATTAATTT CAAATTCAGT TTCTGCTTCA CTTGAGAAAT TTGCGTCAGC GTTGGATTTA 
GAAGCTTGTT TCTCTTCTTC CAAACCAGCG GAAGTTGCCA AGCTATTGTT AGAAGTGTAC 
TCAAGTCAAG ATACCCCTAC TAAATTAAAC GAATGGGCAA ACTATTTTAT GCTTTGTTTA 
ATACGCCACG CTACTCGTGA TTCCCCTGCC AAGCAAGCCC CACAATTTCA ATCAAAATCT 
CCTGAATGTG TAATTATTAG TACTATGAAT CGCAGTTGCG ATTCAAAGTG CCGCTATTTC 
TTGTTAACTG CGATTGCTAA TCAGCTGCGT TACCCTAGCA GCCATACTTA CTATGCTAGC 
TGTTGTTTCT TGTACCTTTT CAAGAGTTCT TCTAACAACC CTCAAGAATT GCTTATCAAA 
GAACAGATGA CAACTGTACT TTTGGAACGC ATAATTTGCA ATCGACCACA TCCTTGGGGC 
CTTTTAATTA CATTTACTGA ATTATTAAAA AATGAAGACT ATAACTTTTG GAAACACCCT 
TATATAAAGC GAAACGATGA AATCTGTCGT TTGTTTGATA GTCTCCATGA ACATGTCATG 
GCACCCTCCT CAGCTAATAA TGTCTCTACC GAAGAGGCTA CAGAACTGAT GGGTCAAGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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not1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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