pks-5 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	pks-5
Entrez Gene ID	3874316
Full Name	polyketide synthase-5
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_953076.1	XP_958169.1	polyketide synthase 5

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:dkey-61p9.11	NP_001041530.1
Danio rerio (zebrafish)	wu:fc01d11	XP_682975.3
Neurospora crassa	NCU09638	XP_958169.1
Gallus gallus (chicken)	LOC420486	XP_418588.4

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pks-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pks-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf00185	XM_953076.1 Latest version!	Neurospora crassa OR74A polyketide synthase 5 (pks-5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pks-5 ( XM_953076.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_958169.1 Neurospora crassa OR74A polyketide synthase 5 (pks-5), partial mRNA.

Price & Availability↑

CloneID	ONf00185
Clone ID Related Accession (Same CDS sequence)	XM_953076.1
Accession Version	XM_953076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	polyketide synthase 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). COMPLETENESS: incomplete on both ends.

ATGTCTTCCA CCTTCAAGCA CGACCCTCAT GAGCCGGTGG CCATTGTGGG CATGGCATGC 
CGATGGCCAG GCGGTGTTCA CGACCCCTCT CAATTCTGGG AGTTTCTCCG GAACAAAGTC 
AACGGCTGGA AGGAGTTTGA TGACCCGCGC TTCTCTTCAG CCGGGTTTCA CCATCCCAAC 
TCGGACCGGC CGGGCTCCAT GTCCATGAAG GGCGCTTTTC TTGCTGAGCA GGATGCTCGT 
CTTTTTGATC ACACCTTCTT CGGCATGACC GGTCTGGAAG TCGAAACCAT GGACCCTTCG 
CAACGCAAGT TGCTTGAGGT GGCCTATGAA GCCATCGAGA GTGCCGGAGA GACGTGGGAG 
AGCGTTTCGG GGACCCGGAC GGGAGTCTTT GTTGGCAACT TCTGCCTTGA TCACTGGATG 
ATTCAATCGC GGGATTGGGA CAACCCGAGG CCGTATGCCT TTACTGGCGC CGGCACCAGC 
ATTCTTGCCA ACCGCATCAG CTATATCTTC AATCTTCAAG GGCCAAGCCT CACGGTGGAC 
ACCGCCTGCT CGTCCTCCAT GTACGCCCTC CACCTCGCAG TCAACGCCAT CCGCGCCGGG 
GACTGTGATT CGGCCATCGT CGCCTCGGCC AACTGGATCG CCGATCCCGG GGTGCAAATT 
GCCTTGGACA AGCTCGGCGC ACTCTCCGCA TCTGCGCGCT GCCACACCTT CGACGCTCGG 
GCGGAAGGCT ACGCTCGCGG CGAGGGATTC GGCGCCATTT ATCTCAAGAG ACCTTCCCTC 
GCTATCGCCG ATATGTCACC CATCCGCGCA ATGATCCGAG GAACAGCCAT CAACTCCAAT 
GGACGAACCG GCGGCATCAC GAGGCCTAGT GCCAACGGAC AAGAGACGGT CATCCGGGAA 
GCATACCGCA ACGCCGGCAA TCTGCCGTTC CGCGACACCA GCTACTTTGA ATGCCATGGA 
ACAGGCACGT ATGTCGGAGA CCCAATCGAA GTAGCCGCAC TAGGAAGAGT CTTTGCTCCA 
GAACGGTCTA GCGACGATCC GCTACTTATC GGTTCCGTGA AAAGTAACGT GGGACACGGG 
GAAGGTGCCA GCGCTTTGGC GTCCATTATG AAGGTTGTTC TTGCTCTAGA GCACGGTGCA 
ATCCCACCCA TCTACGATCT TCAAACCCGG AACCCCAACA TCGACTTCGA GGGTGCCAAG 
GTTCAGCCAG TGACCGAGGT CACGCCGTGG CCCAAGGATC GCCTGCAGAG AGCAAGCATC 
AACTCGTTCG GTTATGGTGG TGCCAACGGT CATTGCATCA TCGACCATGT CAACATCGTG 
CTGCCGGATT ATGTTGCGCC AGGGATCTAC AAGCGCAGCA CCAGAGATAC AACAAACGGT 
GTGAATGGTC ATATGAACGG GCGCAACGGT TCGCCTTCGC ATCGTCCCAT GATTCAGAGG 
CCAAAGATGA CCGCCTCGCC GAATGCCAAC ACACGACCAC TTGTCCTCCT CCCCCTCTCT 
GCTCACAACG AAAACTCTCT CGAGCTGAAC CTCAAAGCGT TGTCTCAAGT TGTCGACAAG 
TTACCGTTGG CAGATGTCGC ATACACCTTG GGTGCCAGAC GGTCCAAATT TGCCCAGCGA 
TCCTTCTGCA TCGTAGAGAA AGACAAGGTT GTTGAGGGCC TCGCGGCCAA GAGCAGGGTG 
GTAAGAGCAC CATTGCAACC CACCAACCTT GGCTTCATTT TCACCGGCCA AGGCGCTCAG 
TGGCATGCCA TGGGTGCCCA GCTGTTTGAG TATCGAGTGT TCTCCACAGC GATCCGGTAC 
CTGGATCATG TCCTCAGCTC GCTGCCCAAT GGTCCGGACT GGTCACTAGA GAAAATCCTG 
TCCGGAGACT GTGATGCTGC CCTCATCCAG AGAGCCGAGA TTTCTCAGGC TGTTTGCACT 
GCTGTACAAG TTGGCCTGGT TGATCTGCTT GCCTCGTGGT CCGTGAGACC TCACAGTGTT 
GCTGGACATT CTTCCGGTGA AATGGCTGCG GCGTACGCTG CTGGTCGTAT CACAGCTGCC 
GAAGCCATCG TAGCCGCTTA CTTCCGCGGT CAGGCGGTAT CACGGAACCG ACAGACCGGT 
GCAATGCTGG CTGTCGGCCT GGGCCCTGAA GCGGTAGCCA AGTATCTCTC TGGCCTGGAG 
GATCAGGTCA AGGTAGCAGC AATCAACTCG CCAGGAAGCG TCACGTTGTC CGGCGATCCA 
GCAGCCATTG ATAGTATCTC GAAAGCCTTG GCCGCCGACA GCATCTTCAA TCGAAAACTC 
CAGACGGGCG GCAATGCTTA CCACTCACAC CACATGCTTC CCATTGGGCG TGAGTACATC 
GAGATGCTGA GCCAGGGCCT TCAACACATC CAGAAGCTCG GCCTTGCCTC ACCAGAGCAG 
CGGTATCCCA AGGCATTGTG GGTATCGTCT GTCAAACCCA ACAAAGACAC AACTGAGTCG 
TTTGACGATC CATCCGCTTA CTGGAGGGCC AATCTCGAGT CACCTGTGCA GTTCTCGGAG 
GCTGTTGCAA GCTTGGTACG CAACGAGAAT GTCACCATCC ACGCTTTGGT AGAGATTGGA 
CCGCACCCCG CGCTGAAGAG CCCGGTGGAG CAGATTGTCA AGGCCGCTGG CAAGGCTGTG 
GCGTATGCCT CCACGCTCAA GAGGCAGGAG GACGCCCGGC CATCCATGTT GCAACTCGCC 
GGAACGCTGT TCACTTTAAA CACGCCCATC GACCTTGCCG CCGTAAATGC TGTTGATGGG 
GAAGCAGGAC ACAGCGGAAG CCTAGAACAC GGCAGCACCT GTCTCGATCT GCCACCCTAC 
CAGTACACTT ATAACGGCCT GAACTATCAT GAGAGCCGGG CCAGCAAGGA GTACCGGTAC 
CGGTCGGTGT TGAGACATGA CCTTCTCGGC TCGAAGGTCG TCGGCAATGC AAAGTTGCGG 
CCCCAATGGC GGAACATTCT CCGGATGAAG GACGTCCCAT GGTTAGGCGA TCACCGCCTT 
GTGCCAGATG CCGTCCTGCC AGGTGCCGCG TACATCGCTA TGGCGGTAGA AGCTGCGTCA 
CGCATCTACG ATGAGTTCCC CAAGCCGTTC GAGGTCAAGG GCTTCTCTCT ACGTGACGTC 
GTCATCAAGA AGAGCCTTGT AATCCCAGAA GACGACTACG GCGTTGAGAT ACTCACGAGC 
ATGGAGCTTG TCGACCTAGC CACAGCGCAA TCACCTGCAT GGGCTACCTT TTCCATCAGC 
TCAGTTGGCC GCGAAACCAA TGAGTGGACG GAGCACTGCA CTGGACGAGT CAAGGTGGTG 
GTTCAAGAGA CAGGCTATGA TATTGACGTC GAACACGGTC GAGTTGCCGC TCCGTACGCA 
CCAAGGGCCG TGGACGTTAA GGCTTGGTAC AAGAAGTTTT TGGACATTGG CCTCGGCTAC 
GGTCCGGTGT TCCAGCCTCT GTCGGACATC CGTGTTGATG CGGCGTCAAA CTTGGCCGTG 
GCGACCGTTC ATCTGCAGCC CGGGATTGGT GTCATGAAGG GTGGCGAGTC CCGGTACGCC 
GTACACCCGG CCTCCCTGGA CGGTGCCATT CAGCTGGGCT TGGTTGCGTG CCACGGAGGC 
CGGCCGAGCG AGGCTACCAC TGCCTTCGTA CCGGTACAAT TCTCCAGTTT GTACATCGAC 
AACAGCCTAG CCAGTGGCGG CGAGACATGT ACCATTGTGG CTCGTGGGCA GCGGCGCGGT 
ATCCGGAGTG CCCATCTTGA TCTGCAGTTC CTGGGTGCAG ATGGAAAGCT TCTGCTTGAC 
GCCGAGTCCC TCCGATGCGT TAGCTTCTCC AGTGAGGCCA AGCCGGTGGA TAGGGCATTC 
AGCAGCCCCT ACACTCGCCT AGTGTGGAAG CCGGACATCC GCAAGCTCAG CAACAGCCAA 
GCTCGCCAGA TATACCCTCC ACCAACCAAG AATGTTGACA AGGCGCCACT GTGGGGGATC 
ACGAACAAGG TAGCCCACTT TATTGTATAC AGCATGTATG AAACTTTCGG GCGGCAGGCA 
GATCGGCCCC AGCCTTCGGG TGAGGTCGGC CACTTCTTTG ACTGGATCAT CAGAAAGGGT 
GAAAGTGATC ATTCTGAGAT GATGGAAGAG GCAAGAGAGC TGGCAGCTAA GGGTGCCCTG 
CTTCAGCAGA TCGAAGAACT TGTCGCCCAG GCACCTGACA TTATGGAAGT CAAGATCGCA 
AAGCTTCTGC ACGACAATGC GGCCGACATT CTGTATGAAC GAAAAACGGG GATGGAAGTC 
ATCATCGGGG AGAACCTTCT GACACCACTT TATCAGGAAG GCCTCCTGAT GACAGGCATC 
TATCCACAGC TCTCCATTGT CCTAGCTGGC CTTGCCCACG CCAACCCAAA CCTCCGGATT 
CTCGAGATTG GAGGCGGTAC TGGTGGCGCG ACCCGGATCG CCATGAAGGC TTTTAACGGC 
CCCAACGGCA TCAAGGCTTA CCGGGATTAC ACCTTCACAG ACATCTCGCC CGGCTTCCTG 
TCGGCGGCCA AGGAATCCAT GGCCGATCTG AGGGATATGA ACTTCTCCGT CTTTGACATT 
GAAGAGGACC CAGTGGCACA GGGATACGAG GAGCAGACAT ATGACCTGAT TATCGCGTGT 
CAAGTCCTTC ATGCCACCTC GAACATGACC AAGACGCTGT CAAGATGCCG CAAGCTCCTC 
AAGCCAGGTG GTCGACTCGT TCTGGTTGAG ACCACGGAGA ACTTCATCGT GCCAGGCGTT 
GTTGTGGGCA CCTTTACGGG ATACTGGGCC GGCATTCCAG ATGGACGTGT GGATGCTCCT 
TTCCAGAGCC TGAAGGACTG GGACCGTTGC CTGAGAGAAG CAGGCTTCGC TGGGCTGGAC 
CTGGTACTTG ATGACTTCCC GGAACCCCAT AATACCACAT CGGTTATTCT TGCCAAGGTG 
CCTGAGCAAG AGCCTGAACC GTTGCCGGCA GCCCAGTCCG CAACGACTGT CCATGTGGTG 
CATGGTGGCA GCAACGGAAC ACCAGTTCTG GTAGATCATA TATGTCAAGA ACTGCAACAG 
CGGGGTACCA ATGTAAAGGC TATCACGTTC AACGACGTCA CAAAGGAGCT TTCTCCCGGA 
TCGCGCGTGG TCGTATTGCT GGACGAGAGC CACCTGCTGA TCAAGGCCAA CGAGGAGAAT 
CTGGCTACCA TGCAATACCT GGCACGCAGT GCAACGAGTT TGGTCGCTCT GACAAGTTGC 
GGTACCATCA AGGGACGGGC TGCCGAGGGT GCCTTGATCT CAGGCCTGCT TCGCGTACTC 
CAGACTGAGA ACCCCTCCAG CCAGTACATG TCCATCGATA TCGACGTGGA CAACTTTGAG 
ATTGAAGATG CTGCTGAAGC AAACGAGCTC GCTCGCAGCA TCGTGGACGG CGAGTGTGAC 
CTGTTTCGTC AAGCCAGCGG ATCGGCTCCT GTATTTGTGG ATGGGGAGAG CGATGGTGGC 
GACCCCGCTG ACCGCGAGTT CGTTTGGCAA AACGGTTGCC TGTGGGTTAG CCGTCACGTC 
CCAGATGCCG GATTCCACTC CCAGTACGGC TTAGAGACGA GGAGCATGAA GCCGGAACTA 
CGTCCGATTG GCAGCCCCAG TCAGACAGGG GGAGTACGTG CTGCTTTCGA GACGCCCGGT 
GTGCTGAACT CATTGTACTT CATTCCCTAC AAAGAACTCC TGCAGCCGTT GCCGGCCGAC 
TTCATCGATG TGGCTGTTGA TGCTGTGGGC GTAAATTCAA GAGATTTGGA GCACTGGGCT 
GGCCGCGTGG ATGGCGATTA CATGACTTCC GAATATGCGG GTGTGGTCAC CGCGGTAGGC 
AGCCGAGTCC ACAACATCCA GGTTGGCGAT CGCGTCTATG GAGTGGGCAA GGGTCAGTTT 
GGCAATGTCA CCCGTGTGCC TGCTGCCTTC GCAACGAAGC TTCAGCCCAA CGATGACATG 
ATCCGGATGG CCTCAATGCC GCTCGCATAC ACCACCGCCG TCTACGCCTT GGACCACGTC 
GCTCACGTCC GTGAGGGACA GTCGATCCTG ATACAGTCGG GCGCCAGGGA CCTTGGCCTG 
GCAGCCATCA CTCTTGCGAA GGCAAAGCGG TCACAGGTGT TTGCAACAGT TGAGACAGCC 
GAGCAAGGCA AGTTCCTAGT TGAGGAGCTA GGCCTACCCG CCTCGCATGT CATTTCTATC 
CTCGACTCGA AGCAGCTCCA GCGCGCAGCT GAGGAAACGC GCAAGAGAAA GTTCGAGGTT 
GTTATGGTGA CTGCCGCTCG TGGGGAGCTC CTGTCTTTGT TCCTGCAGGT CCTGGCACCG 
CTTGGGTGCC TTGTCGATAT CGGCGGGGTG GATGGGCAGA CGACGCCAGT AGTCGGTCAG 
ATGTTACTCC CACACAATGC CACCTACGCC TCTATCGACC CGTTCGCCGT TTTCGATTCG 
GATCCACTGC TTGGGGCGGA GCTCATGCAA TCCGTCGACG ATTACTACCG GCAAGGGGTG 
ATTGGACCGG TTCCAAGAAT AACGGCGGCC GACGTTGGAC AATTGTCGTC GGCACTTGGC 
GATTTTGGCA CAGTATCTGG AAAGCTAGTG GCCAGCTTCA AGAACCCGGA AAGTTTGGTT 
CGGATGGCGC CATTTGCACC CACCGTCAAA TTCGACCCCG AAGCGAGCTA TGTGGTGACG 
GGAGGCTTGG GGGGCTTGGG ACAATCTTTG GTCAGGTGGA TGGTGGGAAG GGGCGCAAGG 
CACCTCGCAC TTTTGTCGAG GCGTACTGCC TCCAGCGTTC CAAGTGTCCA GGAGCTGGTC 
AATGGGCTCG CCGGGCGAGG TGTTGAGATT CAATGCTTTG CCTGCGATGT CAGCAAAGAA 
GACGAGGTGA CCAGCGCGAT CAAGGCAATC TCGGCTGTTC GTCCCATCAA GGGCATCGTG 
CATGCGGCAG TCTCGTATAG GGATCTGACC TTTGACAAGC TGTCACCCGA GAGATGGAAC 
GAAGGGTTGT CTGCCAAGGT GGCAGGCACC AAGAACCTGC ACAAAGCGAC TCTGTCGATG 
CCTCTCGACT TCTTTGTCAT GACCACTTCT GCCTTGTCGG TGTATGGTTT CGCGACCCAA 
AGTGCATACA CGGCTGCCAA CAACTTCCAG GATGCCTTTG CACGATACCG CCGACAGCTT 
GGCTTGCCCG CGTCCACTGT GTCGTTTAGC CTGATCAAGG ACGTGACGGA TGTCGGCAGC 
AACGGGATTA CCGTCGACAT GTTTGAACGC AACAAGGCAC TGACCCTGGA TGAGTCTCAG 
TTCCTGGCGC TCCTCGAGCC GGCATTCTTG AATAACCGAA CGGTGACGGA GGAGACTAGT 
GCTGGGCAAT GGTCAGGTCA GGACGATGAT GGGCTTTCTG TCGCAAATTT GCACACATAC 
TTGGATCCCA GAGCAATGGC GGCGAAAAGA CGTGAAGAGA TGGCCAGCGA GACATCCTCG 
TCATCTTCCA CAGTGCCACC ACGGTGGTAC AGTGACGCGC GCGTGTCTTT GATGATGCGC 
GCCTTTGCGG ACGCACAGCG ACACTCAGGA AGGTCACAAA ACGCAGCCGA GGAGGGTTCC 
AACAAGAACA CAATTGCTCA TCTCCGCGCG GAGCTGGAAG CTGCAGTGCG CGAGGGAGAG 
GCTGGGCGAG GGCGGACGGT GGCGCTGGTA CAGGATGCCA TCACCAAGGT GGTGGCCGAG 
ATGCTGTTCG TCGACGTGGA GAGCATTGAC CCGGCCAAGT CGGTGGCAGA GCTGGGTGTC 
GACAGTCTGA TTGCGGCCGA GTTGCGCAAC TGGTTCATCC AGGCTCTGGG GACCAACATC 
AGCATGTTGG ATCTGCTGGA CCCCAGCGTG AGCATCAGCA CCCGCGCTTC CACCATTGCG 
GACAAGGCCC TGACTGTGAC TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_958169.1
CDS	1..7887
Translation	MSSTFKHDPHEPVAIVGMACRWPGGVHDPSQFWEFLRNKVNGWKEFDDPRFSSAGFHHPNSDRPGSMSMKGAFLAEQDARLFDHTFFGMTGLEVETMDPSQRKLLEVAYEAIESAGETWESVSGTRTGVFVGNFCLDHWMIQSRDWDNPRPYAFTGAGTSILANRISYIFNLQGPSLTVDTACSSSMYALHLAVNAIRAGDCDSAIVASANWIADPGVQIALDKLGALSASARCHTFDARAEGYARGEGFGAIYLKRPSLAIADMSPIRAMIRGTAINSNGRTGGITRPSANGQETVIREAYRNAGNLPFRDTSYFECHGTGTYVGDPIEVAALGRVFAPERSSDDPLLIGSVKSNVGHGEGASALASIMKVVLALEHGAIPPIYDLQTRNPNIDFEGAKVQPVTEVTPWPKDRLQRASINSFGYGGANGHCIIDHVNIVLPDYVAPGIYKRSTRDTTNGVNGHMNGRNGSPSHRPMIQRPKMTASPNANTRPLVLLPLSAHNENSLELNLKALSQVVDKLPLADVAYTLGARRSKFAQRSFCIVEKDKVVEGLAAKSRVVRAPLQPTNLGFIFTGQGAQWHAMGAQLFEYRVFSTAIRYLDHVLSSLPNGPDWSLEKILSGDCDAALIQRAEISQAVCTAVQVGLVDLLASWSVRPHSVAGHSSGEMAAAYAAGRITAAEAIVAAYFRGQAVSRNRQTGAMLAVGLGPEAVAKYLSGLEDQVKVAAINSPGSVTLSGDPAAIDSISKALAADSIFNRKLQTGGNAYHSHHMLPIGREYIEMLSQGLQHIQKLGLASPEQRYPKALWVSSVKPNKDTTESFDDPSAYWRANLESPVQFSEAVASLVRNENVTIHALVEIGPHPALKSPVEQIVKAAGKAVAYASTLKRQEDARPSMLQLAGTLFTLNTPIDLAAVNAVDGEAGHSGSLEHGSTCLDLPPYQYTYNGLNYHESRASKEYRYRSVLRHDLLGSKVVGNAKLRPQWRNILRMKDVPWLGDHRLVPDAVLPGAAYIAMAVEAASRIYDEFPKPFEVKGFSLRDVVIKKSLVIPEDDYGVEILTSMELVDLATAQSPAWATFSISSVGRETNEWTEHCTGRVKVVVQETGYDIDVEHGRVAAPYAPRAVDVKAWYKKFLDIGLGYGPVFQPLSDIRVDAASNLAVATVHLQPGIGVMKGGESRYAVHPASLDGAIQLGLVACHGGRPSEATTAFVPVQFSSLYIDNSLASGGETCTIVARGQRRGIRSAHLDLQFLGADGKLLLDAESLRCVSFSSEAKPVDRAFSSPYTRLVWKPDIRKLSNSQARQIYPPPTKNVDKAPLWGITNKVAHFIVYSMYETFGRQADRPQPSGEVGHFFDWIIRKGESDHSEMMEEARELAAKGALLQQIEELVAQAPDIMEVKIAKLLHDNAADILYERKTGMEVIIGENLLTPLYQEGLLMTGIYPQLSIVLAGLAHANPNLRILEIGGGTGGATRIAMKAFNGPNGIKAYRDYTFTDISPGFLSAAKESMADLRDMNFSVFDIEEDPVAQGYEEQTYDLIIACQVLHATSNMTKTLSRCRKLLKPGGRLVLVETTENFIVPGVVVGTFTGYWAGIPDGRVDAPFQSLKDWDRCLREAGFAGLDLVLDDFPEPHNTTSVILAKVPEQEPEPLPAAQSATTVHVVHGGSNGTPVLVDHICQELQQRGTNVKAITFNDVTKELSPGSRVVVLLDESHLLIKANEENLATMQYLARSATSLVALTSCGTIKGRAAEGALISGLLRVLQTENPSSQYMSIDIDVDNFEIEDAAEANELARSIVDGECDLFRQASGSAPVFVDGESDGGDPADREFVWQNGCLWVSRHVPDAGFHSQYGLETRSMKPELRPIGSPSQTGGVRAAFETPGVLNSLYFIPYKELLQPLPADFIDVAVDAVGVNSRDLEHWAGRVDGDYMTSEYAGVVTAVGSRVHNIQVGDRVYGVGKGQFGNVTRVPAAFATKLQPNDDMIRMASMPLAYTTAVYALDHVAHVREGQSILIQSGARDLGLAAITLAKAKRSQVFATVETAEQGKFLVEELGLPASHVISILDSKQLQRAAEETRKRKFEVVMVTAARGELLSLFLQVLAPLGCLVDIGGVDGQTTPVVGQMLLPHNATYASIDPFAVFDSDPLLGAELMQSVDDYYRQGVIGPVPRITAADVGQLSSALGDFGTVSGKLVASFKNPESLVRMAPFAPTVKFDPEASYVVTGGLGGLGQSLVRWMVGRGARHLALLSRRTASSVPSVQELVNGLAGRGVEIQCFACDVSKEDEVTSAIKAISAVRPIKGIVHAAVSYRDLTFDKLSPERWNEGLSAKVAGTKNLHKATLSMPLDFFVMTTSALSVYGFATQSAYTAANNFQDAFARYRRQLGLPASTVSFSLIKDVTDVGSNGITVDMFERNKALTLDESQFLALLEPAFLNNRTVTEETSAGQWSGQDDDGLSVANLHTYLDPRAMAAKRREEMASETSSSSSTVPPRWYSDARVSLMMRAFADAQRHSGRSQNAAEEGSNKNTIAHLRAELEAAVREGEAGRGRTVALVQDAITKVVAEMLFVDVESIDPAKSVAELGVDSLIAAELRNWFIQALGTNISMLDLLDPSVSISTRASTIADKALTVTA

Target ORF information:

RefSeq Version	XM_953076.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A polyketide synthase 5 (pks-5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_953076.1

ORF Insert Sequence:

ATGTCTTCCA CCTTCAAGCA CGACCCTCAT GAGCCGGTGG CCATTGTGGG CATGGCATGC 
CGATGGCCAG GCGGTGTTCA CGACCCCTCT CAATTCTGGG AGTTTCTCCG GAACAAAGTC 
AACGGCTGGA AGGAGTTTGA TGACCCGCGC TTCTCTTCAG CCGGGTTTCA CCATCCCAAC 
TCGGACCGGC CGGGCTCCAT GTCCATGAAG GGCGCTTTTC TTGCTGAGCA GGATGCTCGT 
CTTTTTGATC ACACCTTCTT CGGCATGACC GGTCTGGAAG TCGAAACCAT GGACCCTTCG 
CAACGCAAGT TGCTTGAGGT GGCCTATGAA GCCATCGAGA GTGCCGGAGA GACGTGGGAG 
AGCGTTTCGG GGACCCGGAC GGGAGTCTTT GTTGGCAACT TCTGCCTTGA TCACTGGATG 
ATTCAATCGC GGGATTGGGA CAACCCGAGG CCGTATGCCT TTACTGGCGC CGGCACCAGC 
ATTCTTGCCA ACCGCATCAG CTATATCTTC AATCTTCAAG GGCCAAGCCT CACGGTGGAC 
ACCGCCTGCT CGTCCTCCAT GTACGCCCTC CACCTCGCAG TCAACGCCAT CCGCGCCGGG 
GACTGTGATT CGGCCATCGT CGCCTCGGCC AACTGGATCG CCGATCCCGG GGTGCAAATT 
GCCTTGGACA AGCTCGGCGC ACTCTCCGCA TCTGCGCGCT GCCACACCTT CGACGCTCGG 
GCGGAAGGCT ACGCTCGCGG CGAGGGATTC GGCGCCATTT ATCTCAAGAG ACCTTCCCTC 
GCTATCGCCG ATATGTCACC CATCCGCGCA ATGATCCGAG GAACAGCCAT CAACTCCAAT 
GGACGAACCG GCGGCATCAC GAGGCCTAGT GCCAACGGAC AAGAGACGGT CATCCGGGAA 
GCATACCGCA ACGCCGGCAA TCTGCCGTTC CGCGACACCA GCTACTTTGA ATGCCATGGA 
ACAGGCACGT ATGTCGGAGA CCCAATCGAA GTAGCCGCAC TAGGAAGAGT CTTTGCTCCA 
GAACGGTCTA GCGACGATCC GCTACTTATC GGTTCCGTGA AAAGTAACGT GGGACACGGG 
GAAGGTGCCA GCGCTTTGGC GTCCATTATG AAGGTTGTTC TTGCTCTAGA GCACGGTGCA 
ATCCCACCCA TCTACGATCT TCAAACCCGG AACCCCAACA TCGACTTCGA GGGTGCCAAG 
GTTCAGCCAG TGACCGAGGT CACGCCGTGG CCCAAGGATC GCCTGCAGAG AGCAAGCATC 
AACTCGTTCG GTTATGGTGG TGCCAACGGT CATTGCATCA TCGACCATGT CAACATCGTG 
CTGCCGGATT ATGTTGCGCC AGGGATCTAC AAGCGCAGCA CCAGAGATAC AACAAACGGT 
GTGAATGGTC ATATGAACGG GCGCAACGGT TCGCCTTCGC ATCGTCCCAT GATTCAGAGG 
CCAAAGATGA CCGCCTCGCC GAATGCCAAC ACACGACCAC TTGTCCTCCT CCCCCTCTCT 
GCTCACAACG AAAACTCTCT CGAGCTGAAC CTCAAAGCGT TGTCTCAAGT TGTCGACAAG 
TTACCGTTGG CAGATGTCGC ATACACCTTG GGTGCCAGAC GGTCCAAATT TGCCCAGCGA 
TCCTTCTGCA TCGTAGAGAA AGACAAGGTT GTTGAGGGCC TCGCGGCCAA GAGCAGGGTG 
GTAAGAGCAC CATTGCAACC CACCAACCTT GGCTTCATTT TCACCGGCCA AGGCGCTCAG 
TGGCATGCCA TGGGTGCCCA GCTGTTTGAG TATCGAGTGT TCTCCACAGC GATCCGGTAC 
CTGGATCATG TCCTCAGCTC GCTGCCCAAT GGTCCGGACT GGTCACTAGA GAAAATCCTG 
TCCGGAGACT GTGATGCTGC CCTCATCCAG AGAGCCGAGA TTTCTCAGGC TGTTTGCACT 
GCTGTACAAG TTGGCCTGGT TGATCTGCTT GCCTCGTGGT CCGTGAGACC TCACAGTGTT 
GCTGGACATT CTTCCGGTGA AATGGCTGCG GCGTACGCTG CTGGTCGTAT CACAGCTGCC 
GAAGCCATCG TAGCCGCTTA CTTCCGCGGT CAGGCGGTAT CACGGAACCG ACAGACCGGT 
GCAATGCTGG CTGTCGGCCT GGGCCCTGAA GCGGTAGCCA AGTATCTCTC TGGCCTGGAG 
GATCAGGTCA AGGTAGCAGC AATCAACTCG CCAGGAAGCG TCACGTTGTC CGGCGATCCA 
GCAGCCATTG ATAGTATCTC GAAAGCCTTG GCCGCCGACA GCATCTTCAA TCGAAAACTC 
CAGACGGGCG GCAATGCTTA CCACTCACAC CACATGCTTC CCATTGGGCG TGAGTACATC 
GAGATGCTGA GCCAGGGCCT TCAACACATC CAGAAGCTCG GCCTTGCCTC ACCAGAGCAG 
CGGTATCCCA AGGCATTGTG GGTATCGTCT GTCAAACCCA ACAAAGACAC AACTGAGTCG 
TTTGACGATC CATCCGCTTA CTGGAGGGCC AATCTCGAGT CACCTGTGCA GTTCTCGGAG 
GCTGTTGCAA GCTTGGTACG CAACGAGAAT GTCACCATCC ACGCTTTGGT AGAGATTGGA 
CCGCACCCCG CGCTGAAGAG CCCGGTGGAG CAGATTGTCA AGGCCGCTGG CAAGGCTGTG 
GCGTATGCCT CCACGCTCAA GAGGCAGGAG GACGCCCGGC CATCCATGTT GCAACTCGCC 
GGAACGCTGT TCACTTTAAA CACGCCCATC GACCTTGCCG CCGTAAATGC TGTTGATGGG 
GAAGCAGGAC ACAGCGGAAG CCTAGAACAC GGCAGCACCT GTCTCGATCT GCCACCCTAC 
CAGTACACTT ATAACGGCCT GAACTATCAT GAGAGCCGGG CCAGCAAGGA GTACCGGTAC 
CGGTCGGTGT TGAGACATGA CCTTCTCGGC TCGAAGGTCG TCGGCAATGC AAAGTTGCGG 
CCCCAATGGC GGAACATTCT CCGGATGAAG GACGTCCCAT GGTTAGGCGA TCACCGCCTT 
GTGCCAGATG CCGTCCTGCC AGGTGCCGCG TACATCGCTA TGGCGGTAGA AGCTGCGTCA 
CGCATCTACG ATGAGTTCCC CAAGCCGTTC GAGGTCAAGG GCTTCTCTCT ACGTGACGTC 
GTCATCAAGA AGAGCCTTGT AATCCCAGAA GACGACTACG GCGTTGAGAT ACTCACGAGC 
ATGGAGCTTG TCGACCTAGC CACAGCGCAA TCACCTGCAT GGGCTACCTT TTCCATCAGC 
TCAGTTGGCC GCGAAACCAA TGAGTGGACG GAGCACTGCA CTGGACGAGT CAAGGTGGTG 
GTTCAAGAGA CAGGCTATGA TATTGACGTC GAACACGGTC GAGTTGCCGC TCCGTACGCA 
CCAAGGGCCG TGGACGTTAA GGCTTGGTAC AAGAAGTTTT TGGACATTGG CCTCGGCTAC 
GGTCCGGTGT TCCAGCCTCT GTCGGACATC CGTGTTGATG CGGCGTCAAA CTTGGCCGTG 
GCGACCGTTC ATCTGCAGCC CGGGATTGGT GTCATGAAGG GTGGCGAGTC CCGGTACGCC 
GTACACCCGG CCTCCCTGGA CGGTGCCATT CAGCTGGGCT TGGTTGCGTG CCACGGAGGC 
CGGCCGAGCG AGGCTACCAC TGCCTTCGTA CCGGTACAAT TCTCCAGTTT GTACATCGAC 
AACAGCCTAG CCAGTGGCGG CGAGACATGT ACCATTGTGG CTCGTGGGCA GCGGCGCGGT 
ATCCGGAGTG CCCATCTTGA TCTGCAGTTC CTGGGTGCAG ATGGAAAGCT TCTGCTTGAC 
GCCGAGTCCC TCCGATGCGT TAGCTTCTCC AGTGAGGCCA AGCCGGTGGA TAGGGCATTC 
AGCAGCCCCT ACACTCGCCT AGTGTGGAAG CCGGACATCC GCAAGCTCAG CAACAGCCAA 
GCTCGCCAGA TATACCCTCC ACCAACCAAG AATGTTGACA AGGCGCCACT GTGGGGGATC 
ACGAACAAGG TAGCCCACTT TATTGTATAC AGCATGTATG AAACTTTCGG GCGGCAGGCA 
GATCGGCCCC AGCCTTCGGG TGAGGTCGGC CACTTCTTTG ACTGGATCAT CAGAAAGGGT 
GAAAGTGATC ATTCTGAGAT GATGGAAGAG GCAAGAGAGC TGGCAGCTAA GGGTGCCCTG 
CTTCAGCAGA TCGAAGAACT TGTCGCCCAG GCACCTGACA TTATGGAAGT CAAGATCGCA 
AAGCTTCTGC ACGACAATGC GGCCGACATT CTGTATGAAC GAAAAACGGG GATGGAAGTC 
ATCATCGGGG AGAACCTTCT GACACCACTT TATCAGGAAG GCCTCCTGAT GACAGGCATC 
TATCCACAGC TCTCCATTGT CCTAGCTGGC CTTGCCCACG CCAACCCAAA CCTCCGGATT 
CTCGAGATTG GAGGCGGTAC TGGTGGCGCG ACCCGGATCG CCATGAAGGC TTTTAACGGC 
CCCAACGGCA TCAAGGCTTA CCGGGATTAC ACCTTCACAG ACATCTCGCC CGGCTTCCTG 
TCGGCGGCCA AGGAATCCAT GGCCGATCTG AGGGATATGA ACTTCTCCGT CTTTGACATT 
GAAGAGGACC CAGTGGCACA GGGATACGAG GAGCAGACAT ATGACCTGAT TATCGCGTGT 
CAAGTCCTTC ATGCCACCTC GAACATGACC AAGACGCTGT CAAGATGCCG CAAGCTCCTC 
AAGCCAGGTG GTCGACTCGT TCTGGTTGAG ACCACGGAGA ACTTCATCGT GCCAGGCGTT 
GTTGTGGGCA CCTTTACGGG ATACTGGGCC GGCATTCCAG ATGGACGTGT GGATGCTCCT 
TTCCAGAGCC TGAAGGACTG GGACCGTTGC CTGAGAGAAG CAGGCTTCGC TGGGCTGGAC 
CTGGTACTTG ATGACTTCCC GGAACCCCAT AATACCACAT CGGTTATTCT TGCCAAGGTG 
CCTGAGCAAG AGCCTGAACC GTTGCCGGCA GCCCAGTCCG CAACGACTGT CCATGTGGTG 
CATGGTGGCA GCAACGGAAC ACCAGTTCTG GTAGATCATA TATGTCAAGA ACTGCAACAG 
CGGGGTACCA ATGTAAAGGC TATCACGTTC AACGACGTCA CAAAGGAGCT TTCTCCCGGA 
TCGCGCGTGG TCGTATTGCT GGACGAGAGC CACCTGCTGA TCAAGGCCAA CGAGGAGAAT 
CTGGCTACCA TGCAATACCT GGCACGCAGT GCAACGAGTT TGGTCGCTCT GACAAGTTGC 
GGTACCATCA AGGGACGGGC TGCCGAGGGT GCCTTGATCT CAGGCCTGCT TCGCGTACTC 
CAGACTGAGA ACCCCTCCAG CCAGTACATG TCCATCGATA TCGACGTGGA CAACTTTGAG 
ATTGAAGATG CTGCTGAAGC AAACGAGCTC GCTCGCAGCA TCGTGGACGG CGAGTGTGAC 
CTGTTTCGTC AAGCCAGCGG ATCGGCTCCT GTATTTGTGG ATGGGGAGAG CGATGGTGGC 
GACCCCGCTG ACCGCGAGTT CGTTTGGCAA AACGGTTGCC TGTGGGTTAG CCGTCACGTC 
CCAGATGCCG GATTCCACTC CCAGTACGGC TTAGAGACGA GGAGCATGAA GCCGGAACTA 
CGTCCGATTG GCAGCCCCAG TCAGACAGGG GGAGTACGTG CTGCTTTCGA GACGCCCGGT 
GTGCTGAACT CATTGTACTT CATTCCCTAC AAAGAACTCC TGCAGCCGTT GCCGGCCGAC 
TTCATCGATG TGGCTGTTGA TGCTGTGGGC GTAAATTCAA GAGATTTGGA GCACTGGGCT 
GGCCGCGTGG ATGGCGATTA CATGACTTCC GAATATGCGG GTGTGGTCAC CGCGGTAGGC 
AGCCGAGTCC ACAACATCCA GGTTGGCGAT CGCGTCTATG GAGTGGGCAA GGGTCAGTTT 
GGCAATGTCA CCCGTGTGCC TGCTGCCTTC GCAACGAAGC TTCAGCCCAA CGATGACATG 
ATCCGGATGG CCTCAATGCC GCTCGCATAC ACCACCGCCG TCTACGCCTT GGACCACGTC 
GCTCACGTCC GTGAGGGACA GTCGATCCTG ATACAGTCGG GCGCCAGGGA CCTTGGCCTG 
GCAGCCATCA CTCTTGCGAA GGCAAAGCGG TCACAGGTGT TTGCAACAGT TGAGACAGCC 
GAGCAAGGCA AGTTCCTAGT TGAGGAGCTA GGCCTACCCG CCTCGCATGT CATTTCTATC 
CTCGACTCGA AGCAGCTCCA GCGCGCAGCT GAGGAAACGC GCAAGAGAAA GTTCGAGGTT 
GTTATGGTGA CTGCCGCTCG TGGGGAGCTC CTGTCTTTGT TCCTGCAGGT CCTGGCACCG 
CTTGGGTGCC TTGTCGATAT CGGCGGGGTG GATGGGCAGA CGACGCCAGT AGTCGGTCAG 
ATGTTACTCC CACACAATGC CACCTACGCC TCTATCGACC CGTTCGCCGT TTTCGATTCG 
GATCCACTGC TTGGGGCGGA GCTCATGCAA TCCGTCGACG ATTACTACCG GCAAGGGGTG 
ATTGGACCGG TTCCAAGAAT AACGGCGGCC GACGTTGGAC AATTGTCGTC GGCACTTGGC 
GATTTTGGCA CAGTATCTGG AAAGCTAGTG GCCAGCTTCA AGAACCCGGA AAGTTTGGTT 
CGGATGGCGC CATTTGCACC CACCGTCAAA TTCGACCCCG AAGCGAGCTA TGTGGTGACG 
GGAGGCTTGG GGGGCTTGGG ACAATCTTTG GTCAGGTGGA TGGTGGGAAG GGGCGCAAGG 
CACCTCGCAC TTTTGTCGAG GCGTACTGCC TCCAGCGTTC CAAGTGTCCA GGAGCTGGTC 
AATGGGCTCG CCGGGCGAGG TGTTGAGATT CAATGCTTTG CCTGCGATGT CAGCAAAGAA 
GACGAGGTGA CCAGCGCGAT CAAGGCAATC TCGGCTGTTC GTCCCATCAA GGGCATCGTG 
CATGCGGCAG TCTCGTATAG GGATCTGACC TTTGACAAGC TGTCACCCGA GAGATGGAAC 
GAAGGGTTGT CTGCCAAGGT GGCAGGCACC AAGAACCTGC ACAAAGCGAC TCTGTCGATG 
CCTCTCGACT TCTTTGTCAT GACCACTTCT GCCTTGTCGG TGTATGGTTT CGCGACCCAA 
AGTGCATACA CGGCTGCCAA CAACTTCCAG GATGCCTTTG CACGATACCG CCGACAGCTT 
GGCTTGCCCG CGTCCACTGT GTCGTTTAGC CTGATCAAGG ACGTGACGGA TGTCGGCAGC 
AACGGGATTA CCGTCGACAT GTTTGAACGC AACAAGGCAC TGACCCTGGA TGAGTCTCAG 
TTCCTGGCGC TCCTCGAGCC GGCATTCTTG AATAACCGAA CGGTGACGGA GGAGACTAGT 
GCTGGGCAAT GGTCAGGTCA GGACGATGAT GGGCTTTCTG TCGCAAATTT GCACACATAC 
TTGGATCCCA GAGCAATGGC GGCGAAAAGA CGTGAAGAGA TGGCCAGCGA GACATCCTCG 
TCATCTTCCA CAGTGCCACC ACGGTGGTAC AGTGACGCGC GCGTGTCTTT GATGATGCGC 
GCCTTTGCGG ACGCACAGCG ACACTCAGGA AGGTCACAAA ACGCAGCCGA GGAGGGTTCC 
AACAAGAACA CAATTGCTCA TCTCCGCGCG GAGCTGGAAG CTGCAGTGCG CGAGGGAGAG 
GCTGGGCGAG GGCGGACGGT GGCGCTGGTA CAGGATGCCA TCACCAAGGT GGTGGCCGAG 
ATGCTGTTCG TCGACGTGGA GAGCATTGAC CCGGCCAAGT CGGTGGCAGA GCTGGGTGTC 
GACAGTCTGA TTGCGGCCGA GTTGCGCAAC TGGTTCATCC AGGCTCTGGG GACCAACATC 
AGCATGTTGG ATCTGCTGGA CCCCAGCGTG AGCATCAGCA CCCGCGCTTC CACCATTGCG 
GACAAGGCCC TGACTGTGAC TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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pks-5 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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