NCU00199 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU00199
Entrez Gene ID	3872731
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_951491.2	XP_956584.1	hypothetical protein

Pathways from BioSystems

Homologies

Neurospora crassa	NCU00199	XP_956584.1
Magnaporthe oryzae (rice blast fungus)	MGG_06426	XP_003717141.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU00199 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU00199 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf05639	XM_951491.2 Latest version!	Neurospora crassa OR74A hypothetical protein (NCU00199), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU00199 ( XM_951491.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_956584.1 Neurospora crassa OR74A hypothetical protein (NCU00199), mRNA.

Price & Availability↑

CloneID	ONf05639
Clone ID Related Accession (Same CDS sequence)	XM_951491.2
Accession Version	XM_951491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_951491.1.

ATGGCCTCCT TGACCGATAA GACATATTTC CCTCCGCTGG AGGAGTGTTT GACGGGCAAG 
AACATAATAC TGTCCTGGAA ACTCGTCGCT TCTGCCCTTG CCGACCAACA GGGCGATCGC 
ATCACCAGCA ATGCCGTCTC TTCCTTTCTA AAAGATGCCT ACGTCCAGCA CCTGCTCAAG 
AACCCCTCCA AGACCTTTGA ACCTCCGACC TCCAAGACGA AAGCCGACTT CGAGACCAAG 
ACCGCCGCCA TCAACGTGAC CCAGACGCCC AACGAGCGAT TCAATATCAA GACCATCAGG 
GACGACGCCA TATGGCTCAG CACCAATGTC AACATCAGCG AAGTTGCCGC CCTGCGCATC 
GTCGTAGTCG AGTTCCAGTC GCGCCCACAA GCCCACCTCG TCGGCCCTCT CTCTACCCAG 
GACATCGACA ATATTCAGGA AGCTGCTGGA GTCAGTGATG CGCAAACTTC CAACATTCTC 
GTCCTCCTCA ACCTCTCCAC CGTTGCCGAC GCCGAAACCA CATGGGCCGA GTTTGAGAAG 
GATGCCAGCC GCCGCCAGCG AATTCTGGCC CACTACCTCT CCGAACGCCG GTCCTTCGTG 
AGCTCCGCCG AGTCACTCGT AACCTTCATG CTGCACTCGA GTGCTGCCAT CCTGAACCAG 
GAGACGCATA ACTTGCGACG GGAGATTGTG AAGGAGGCAT TCGGATATGA CGAGTTCGAC 
GACCACTCCG AAATCAACAT CAGCTTGTTC GAGGCCCTTG TACCACGTTA CTTCACCCTC 
CTCGAAGATG CCATCAAGAG CGCGCAGACC GGCCCGGCAA ACGTGGAAGA ACGTCTTCTT 
ACCGAGCAGA TCAACATTGA CTGGACCAGG ACGGCTCTCA CCGAGGCTAT CCATGCCATG 
ACGGTCTCTT TCCAGATCCT CGACCTCTCG GGCCCCGTGT TTACTACCCC GGAGATAATA 
ACTCAGTGGT ATCAGATCAT GAACACGTAC GAGTTCCTAG ATGGCATCAG CAGCGGCTAT 
GACCTTGTTT CCGAGCTCGT CATGCCCTTG AAGAGCTTGG TGTGCGTGAT CTCGCTCAGA 
CTCATCAACC TCAACCGATC ACTCCTGTAC TTGAGCCAAG ACATCGACTT GTTGGACTTC 
GAAGAGCCCT ATCTAGCATC ATCCGAGCTT CTAACAATAA TTCATACTGC TGTGACCAAC 
GCCGCAAATG CCGGCTCGCC AACTGCGAGC CCCGTTGTCT TGGCATGGTC CTTGATTCTG 
CAGGAGATGT TTATTGGCTA CCAGGAACGC GTCGAACGAC GGGATGTTGC TCAAAATGCT 
CGCGCTCAAG CCAGTTTCGA ATTGGAGATT CAGCCGAACC CCAGCGAGCG TAGGAACTCG 
GCGGGAAGCA TAGTGTCTAT CGAAAGGTCG CCATATGATC TATTCCTGAC CTCGCAGGCC 
CTTGAGCGGG ATAACCAGTT GGTTGAGACG CTCGCCTTTG CGGCCACCGC TCGTGGCCAG 
GTATACGAAG TCATGTCAGA AATGGCAGCC TGCCTTGGCC ACAGTCAGCT CGCCGCCTTC 
TCTTCCCTGT TAGGAGCGCG CGCGCGGGCC GTTTTCCTCG AAGTTCTCAA GAAGAGCTTC 
CCTATCGTGG GGTACCAGAA CGATACCGTT TCCTGCCTCC TTACCGTACT GTCCGGAGGG 
AGGCAGTACT GGGATATTTC TCCTAAGAAT GCACTGTCCC CAAACCAGGA AGTTGATTCT 
GTCATGCTTC GGGATGTCGA GCTCAAGGAA AACTATCTCT TGCAGGCTCG CAGCCGTTAC 
CCTTACGAAT TCCTGCCCTT CGTCTCATTC ACTCGGGCTC TGTTGACGGG CCTCCTCGCA 
GACAGCGAAA ACTCCGAGTG GGTGATCGGT CTGCTCCTCA AATCTCCCAC CTTGACGTTG 
GAATGGAGTC CTGACTGGGT TAATTACGAA CTCATCTTCG AGGATGAGAA CATCAACTCC 
TTCCAGTTGA TTGGCGACCT TGACCTCTTC GATACCTCCA AGGTTACCAG GAGGCGCCCC 
GAAAAGGAGG AAAAGTTTAC CATTCCTGCC GGCACTGTTG GACGTTTCGT CTCGGATAGC 
GAGAGGATCG CCATACTGGA ATACGAGCAC TCGGCCCTGG CCCTCCTGGG CAAGCGTCTT 
GAGGTGTCTT TGAAGGCTGA CGCATACAGC AGTGCACTCC CGGGACTTAG TGCTGACGAG 
GTGGCGGAAT CGGTACTACT TTTGGCAACC GTTGTCCGAG TCGAGACTCT CAGGACTGCC 
GTGACCGTTT CGAGGGCTGG CTCTGAGGCT GGCATGGCAG TCTTGAAAGA GGCGAGCAGA 
GCGCTACCAC ACACCAAAGA CATCCTGACG GTTGTCTGTG ACCTTATGGA CACCATCATC 
CAAGACGATC TTGTCAACGT TGATGGCCCC AAAACGGCGG TTTTGGCAGC CTGCTTACAG 
TTCCTTGATG CCGCACTGCC TGTGTGCCCA GGCAGGATTT GGGCTTACAT GTCTAGAACT 
GCCCTCATCA ATGGCGAGAC AAGAGCTGGA CGGCTGTCGA GGATTACCGG AAACCTTGAC 
ATGTTGTCTG AGCGCTTCGA CTTCCTTTCT TCCGCCGTCA AGTTTTTCTC GAATCTGATT 
GAAATAGCCA TGCTTGGTGC AGTTCAGCGC AAGGTCGTAA AGGAGACGAA TGCTCGTTCC 
AACGTCGACA CAAACGCATG GCTGGGAACA AGCGAGAAGG TGATCTCTCG GGTCTCTCTC 
GCCATCGCCC AGACCGCGGT CGATATCATC GAGAATTCGG CCACGTGGAG ATTCCCATCC 
GAGCTGGACC GCAGTGTCGT GGTGCGTGAT GTGGTGGGTA TCATGCAGAA GCTCCTTTCA 
TACACCTATA ACCTGAGCGC GAACGGGCCT TCGAATACCT TGACTGGCTC TCTTGAACCT 
GCCGCTAGAT ACGTTCTCGA AAGCTTTGTC ACCGCTTCGT CAAACTCACT CCGATTCCAA 
CCTCTTTTAG CGACACTTCT TGTTGCCTTC CAAATTCCTG ACTCGACTCT TTACCCCCGC 
CGGGCTGCCG TTGTCTCGGA GAGGCTCTTA GTTGTCCTTG ACTTCGCAAC CATCCTCCTC 
CGGGTTTCCA ACTACCTCGA TCTGCCTGTC AGCAAGCTCC AGACGCAGCT GTTCAAATCT 
GCGTCCCTGG TTGCCCGTCT TCCTGCTATC AGGCTGTCCT TCAAGATACC GGCGATAGCT 
CTTCTGAGCG CGCTGGTAGA AAGTGCCGGG AAGGGTAACG CCGAACCTCC ATCACTGCTG 
GGATACCTTG GGCCACAAAT TTCGCGGTCA TTCATCCAGA TTACTTCACA GCTGGATAAG 
CCGTTTGACA GGGTACCAGA GTCGGTCAGT ATATGGAAGT TCTTTTCAAC AATAATGCGG 
AATCGCCAAC AGTGGATGGC AAACTGCCTC CTTACGGGCA AGACGCCTCG CGAGGCCTTG 
AAGGGGGATG GCAAGATTGC AGAAATCTCT CCCGACTCGG TTCTTTCCAC GGCCATCGAA 
AAACTTCGCT CTATCACGAA GTTGCCAAGC CGGGAAGCCA TCGCCATTCT AGACTTTTTC 
ACCTCGGCCC AGAACTACTG GCCTTGGACG ATCTTCGCCA TGCAGAAGGA TACGTCCTTC 
CTGACCGACC TCCGAGCATA TGTCCGCGAT CTCAAGTCGC CCTCGATGGT CTCTAGGACA 
GACCCCAAGG AGGCTGCCTA CCAGGCGAGG ATAGCAGCTT ACATTGCCGA GGCATTTGCC 
ATGCAGCTGT ACCATCTGCG TCAGATGGGG CAATCACAAA AGTTTGCGAA TGACGTCGTG 
AACGATCTCG ATTACTTTTT GCGTAACGGA GTTGAGGTTT CGGGGTACAA CGCCTCGTTG 
CATTCTAACT TTGCAAAGAA CTTTAGCCAA CGGTACCCGG GGATTTCGGT AGAGGATTTC 
AAGAGGACAG TTCTTGTTCC CCGTGATCTG GGTTCCCAAT TTTACTATGC CTTGGATTTT 
GCCGACTTGA TGCTCAGTTA CGACGTGGCA TGGGATGGGT CGCGCAAGAA CGGCTTCAAG 
CATGAGATGG AGAGGGCTAA TGTGAACTTG TCTTTGGTGG AAGCTGAGAT TGCCCTTTTC 
CATGCTTGGG AGTATCTACT TCTAGAGCTT AGCGTCTCGC TTCTCCCCAA GAACGAAACC 
GTCGCCAAGC AGATGGCACA GGTCGCTGAG CAATGTCTGG ATGCCAACCA ACGGGCTCAG 
CCGCCAGACA ACATCTTCGT CCGCTTGGTT CACGCTCGGG CAAACCTGGC TTTGACGCTT 
CTTCAACGCC TCGCTGATGC TTCTCACCTG CCTAAGGACA GCACCAAGCT GCTCACCAGC 
ATCACAAGGG CCATCAATAA CATTGAGGTT CCCTTCGACA AGGATCAGAT CTCCTATTTC 
CGCACCCTCC TCAAGATCCT CTTCGTCGCT CTCCGTGGAA CCAGACACTC GGTCAATGCC 
CCTGCGCCCG GCGCCTCCAC CTTGTCGAAG TCCGGTGGTG AAAGCAGCTC CGTGGCCGTC 
ACCCAACTGA CTCTCAGCAT CCTTGACCGC GTCGTCGCCC AATCCTTCAG GTCCCTCGTC 
GCGCTAGTGC ACGAACCCAC CTCGCCCACC ACGCCCGAGG ACCTTGCCCT CATCACCGCG 
ATCCTGCAAG CCTGCCTGAG CGTCCCCGGC ATTGACCAGT GCCAGGTGCA AATCCTCAAC 
ATCATGGCCA GCCACGACGT GTTCCAAGTC GCCACCTCGC TCTTCTCCTG GGCCGACAAG 
CTGACGGACA AGTCTGGCGA CCCAATTTAC GGCGAGCTTT CCTTGCTCTT CCTGCTTGAG 
CTCTCCGCCA TGCCCGCCAT CGCGGAGCAG CTCGCCTGCG ACGGCCTGCT AGACCACATC 
ACGTCCGCCA ACCTTGCCGG CTTCATGCGC CGCCCCAACG TCGGCCCTCT AGCTGACAAC 
GCTGGCGCCG CCCGCTGCTA TTCCATATGG GCCAAGGGCA TCCTGCCTTT ACTACTCAAC 
ATGCTCGGTG CTTTGGGCGC CACCATCGCC CCTGAAATCG CTTACGTGCT GAATCAATTC 
CCCAATCTGT TGCGCGCGGC CGTGGATCGG TTCGAAGCAC CAGGCACCCA CCGCACCTTT 
TCGCCCTCCA AGTCCGTTTC TATCTCGGGT GGTCCCAACA ATAACAAGGA CATGTCTGGC 
TTCATCACCC TAACCGCCAT TTCGGAAATC CACTCCCTCG CCCTCCTCAC GCGCGTCCTG 
TCAGTCATCA GGATGACCAA TGTCGGCGGC CACTCGGCAA GAGACATCCC GCAGATTACC 
GGAGGGTGGG AAGACGAGGC CAGACAAAAT CTTTTGGAGA ATGTGGAGTT TTGGCTGGGG 
AGCAGGAAGG TGTTGAGGGA ACGGTTGGTG CCTTTGGGGC AGAGGGAGAT GGAGTGGAGG 
AGCCAGAAAG CTTCGGAAGG GTCAGCGTGC GAGAATAAGT TGGAAGAGAA GGCGGTGGAG 
ATGTTGAGTG GTGTGGCGGA GGTGTTGGCG TTGGGTGAGG CGGAGGAGGG TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_956584.1
CDS	459..6035
Translation	MASLTDKTYFPPLEECLTGKNIILSWKLVASALADQQGDRITSNAVSSFLKDAYVQHLLKNPSKTFEPPTSKTKADFETKTAAINVTQTPNERFNIKTIRDDAIWLSTNVNISEVAALRIVVVEFQSRPQAHLVGPLSTQDIDNIQEAAGVSDAQTSNILVLLNLSTVADAETTWAEFEKDASRRQRILAHYLSERRSFVSSAESLVTFMLHSSAAILNQETHNLRREIVKEAFGYDEFDDHSEINISLFEALVPRYFTLLEDAIKSAQTGPANVEERLLTEQINIDWTRTALTEAIHAMTVSFQILDLSGPVFTTPEIITQWYQIMNTYEFLDGISSGYDLVSELVMPLKSLVCVISLRLINLNRSLLYLSQDIDLLDFEEPYLASSELLTIIHTAVTNAANAGSPTASPVVLAWSLILQEMFIGYQERVERRDVAQNARAQASFELEIQPNPSERRNSAGSIVSIERSPYDLFLTSQALERDNQLVETLAFAATARGQVYEVMSEMAACLGHSQLAAFSSLLGARARAVFLEVLKKSFPIVGYQNDTVSCLLTVLSGGRQYWDISPKNALSPNQEVDSVMLRDVELKENYLLQARSRYPYEFLPFVSFTRALLTGLLADSENSEWVIGLLLKSPTLTLEWSPDWVNYELIFEDENINSFQLIGDLDLFDTSKVTRRRPEKEEKFTIPAGTVGRFVSDSERIAILEYEHSALALLGKRLEVSLKADAYSSALPGLSADEVAESVLLLATVVRVETLRTAVTVSRAGSEAGMAVLKEASRALPHTKDILTVVCDLMDTIIQDDLVNVDGPKTAVLAACLQFLDAALPVCPGRIWAYMSRTALINGETRAGRLSRITGNLDMLSERFDFLSSAVKFFSNLIEIAMLGAVQRKVVKETNARSNVDTNAWLGTSEKVISRVSLAIAQTAVDIIENSATWRFPSELDRSVVVRDVVGIMQKLLSYTYNLSANGPSNTLTGSLEPAARYVLESFVTASSNSLRFQPLLATLLVAFQIPDSTLYPRRAAVVSERLLVVLDFATILLRVSNYLDLPVSKLQTQLFKSASLVARLPAIRLSFKIPAIALLSALVESAGKGNAEPPSLLGYLGPQISRSFIQITSQLDKPFDRVPESVSIWKFFSTIMRNRQQWMANCLLTGKTPREALKGDGKIAEISPDSVLSTAIEKLRSITKLPSREAIAILDFFTSAQNYWPWTIFAMQKDTSFLTDLRAYVRDLKSPSMVSRTDPKEAAYQARIAAYIAEAFAMQLYHLRQMGQSQKFANDVVNDLDYFLRNGVEVSGYNASLHSNFAKNFSQRYPGISVEDFKRTVLVPRDLGSQFYYALDFADLMLSYDVAWDGSRKNGFKHEMERANVNLSLVEAEIALFHAWEYLLLELSVSLLPKNETVAKQMAQVAEQCLDANQRAQPPDNIFVRLVHARANLALTLLQRLADASHLPKDSTKLLTSITRAINNIEVPFDKDQISYFRTLLKILFVALRGTRHSVNAPAPGASTLSKSGGESSSVAVTQLTLSILDRVVAQSFRSLVALVHEPTSPTTPEDLALITAILQACLSVPGIDQCQVQILNIMASHDVFQVATSLFSWADKLTDKSGDPIYGELSLLFLLELSAMPAIAEQLACDGLLDHITSANLAGFMRRPNVGPLADNAGAARCYSIWAKGILPLLLNMLGALGATIAPEIAYVLNQFPNLLRAAVDRFEAPGTHRTFSPSKSVSISGGPNNNKDMSGFITLTAISEIHSLALLTRVLSVIRMTNVGGHSARDIPQITGGWEDEARQNLLENVEFWLGSRKVLRERLVPLGQREMEWRSQKASEGSACENKLEEKAVEMLSGVAEVLALGEAEEGA

Target ORF information:

RefSeq Version	XM_951491.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A hypothetical protein (NCU00199), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_951491.2

ORF Insert Sequence:

ATGGCCTCCT TGACCGATAA GACATATTTC CCTCCGCTGG AGGAGTGTTT GACGGGCAAG 
AACATAATAC TGTCCTGGAA ACTCGTCGCT TCTGCCCTTG CCGACCAACA GGGCGATCGC 
ATCACCAGCA ATGCCGTCTC TTCCTTTCTA AAAGATGCCT ACGTCCAGCA CCTGCTCAAG 
AACCCCTCCA AGACCTTTGA ACCTCCGACC TCCAAGACGA AAGCCGACTT CGAGACCAAG 
ACCGCCGCCA TCAACGTGAC CCAGACGCCC AACGAGCGAT TCAATATCAA GACCATCAGG 
GACGACGCCA TATGGCTCAG CACCAATGTC AACATCAGCG AAGTTGCCGC CCTGCGCATC 
GTCGTAGTCG AGTTCCAGTC GCGCCCACAA GCCCACCTCG TCGGCCCTCT CTCTACCCAG 
GACATCGACA ATATTCAGGA AGCTGCTGGA GTCAGTGATG CGCAAACTTC CAACATTCTC 
GTCCTCCTCA ACCTCTCCAC CGTTGCCGAC GCCGAAACCA CATGGGCCGA GTTTGAGAAG 
GATGCCAGCC GCCGCCAGCG AATTCTGGCC CACTACCTCT CCGAACGCCG GTCCTTCGTG 
AGCTCCGCCG AGTCACTCGT AACCTTCATG CTGCACTCGA GTGCTGCCAT CCTGAACCAG 
GAGACGCATA ACTTGCGACG GGAGATTGTG AAGGAGGCAT TCGGATATGA CGAGTTCGAC 
GACCACTCCG AAATCAACAT CAGCTTGTTC GAGGCCCTTG TACCACGTTA CTTCACCCTC 
CTCGAAGATG CCATCAAGAG CGCGCAGACC GGCCCGGCAA ACGTGGAAGA ACGTCTTCTT 
ACCGAGCAGA TCAACATTGA CTGGACCAGG ACGGCTCTCA CCGAGGCTAT CCATGCCATG 
ACGGTCTCTT TCCAGATCCT CGACCTCTCG GGCCCCGTGT TTACTACCCC GGAGATAATA 
ACTCAGTGGT ATCAGATCAT GAACACGTAC GAGTTCCTAG ATGGCATCAG CAGCGGCTAT 
GACCTTGTTT CCGAGCTCGT CATGCCCTTG AAGAGCTTGG TGTGCGTGAT CTCGCTCAGA 
CTCATCAACC TCAACCGATC ACTCCTGTAC TTGAGCCAAG ACATCGACTT GTTGGACTTC 
GAAGAGCCCT ATCTAGCATC ATCCGAGCTT CTAACAATAA TTCATACTGC TGTGACCAAC 
GCCGCAAATG CCGGCTCGCC AACTGCGAGC CCCGTTGTCT TGGCATGGTC CTTGATTCTG 
CAGGAGATGT TTATTGGCTA CCAGGAACGC GTCGAACGAC GGGATGTTGC TCAAAATGCT 
CGCGCTCAAG CCAGTTTCGA ATTGGAGATT CAGCCGAACC CCAGCGAGCG TAGGAACTCG 
GCGGGAAGCA TAGTGTCTAT CGAAAGGTCG CCATATGATC TATTCCTGAC CTCGCAGGCC 
CTTGAGCGGG ATAACCAGTT GGTTGAGACG CTCGCCTTTG CGGCCACCGC TCGTGGCCAG 
GTATACGAAG TCATGTCAGA AATGGCAGCC TGCCTTGGCC ACAGTCAGCT CGCCGCCTTC 
TCTTCCCTGT TAGGAGCGCG CGCGCGGGCC GTTTTCCTCG AAGTTCTCAA GAAGAGCTTC 
CCTATCGTGG GGTACCAGAA CGATACCGTT TCCTGCCTCC TTACCGTACT GTCCGGAGGG 
AGGCAGTACT GGGATATTTC TCCTAAGAAT GCACTGTCCC CAAACCAGGA AGTTGATTCT 
GTCATGCTTC GGGATGTCGA GCTCAAGGAA AACTATCTCT TGCAGGCTCG CAGCCGTTAC 
CCTTACGAAT TCCTGCCCTT CGTCTCATTC ACTCGGGCTC TGTTGACGGG CCTCCTCGCA 
GACAGCGAAA ACTCCGAGTG GGTGATCGGT CTGCTCCTCA AATCTCCCAC CTTGACGTTG 
GAATGGAGTC CTGACTGGGT TAATTACGAA CTCATCTTCG AGGATGAGAA CATCAACTCC 
TTCCAGTTGA TTGGCGACCT TGACCTCTTC GATACCTCCA AGGTTACCAG GAGGCGCCCC 
GAAAAGGAGG AAAAGTTTAC CATTCCTGCC GGCACTGTTG GACGTTTCGT CTCGGATAGC 
GAGAGGATCG CCATACTGGA ATACGAGCAC TCGGCCCTGG CCCTCCTGGG CAAGCGTCTT 
GAGGTGTCTT TGAAGGCTGA CGCATACAGC AGTGCACTCC CGGGACTTAG TGCTGACGAG 
GTGGCGGAAT CGGTACTACT TTTGGCAACC GTTGTCCGAG TCGAGACTCT CAGGACTGCC 
GTGACCGTTT CGAGGGCTGG CTCTGAGGCT GGCATGGCAG TCTTGAAAGA GGCGAGCAGA 
GCGCTACCAC ACACCAAAGA CATCCTGACG GTTGTCTGTG ACCTTATGGA CACCATCATC 
CAAGACGATC TTGTCAACGT TGATGGCCCC AAAACGGCGG TTTTGGCAGC CTGCTTACAG 
TTCCTTGATG CCGCACTGCC TGTGTGCCCA GGCAGGATTT GGGCTTACAT GTCTAGAACT 
GCCCTCATCA ATGGCGAGAC AAGAGCTGGA CGGCTGTCGA GGATTACCGG AAACCTTGAC 
ATGTTGTCTG AGCGCTTCGA CTTCCTTTCT TCCGCCGTCA AGTTTTTCTC GAATCTGATT 
GAAATAGCCA TGCTTGGTGC AGTTCAGCGC AAGGTCGTAA AGGAGACGAA TGCTCGTTCC 
AACGTCGACA CAAACGCATG GCTGGGAACA AGCGAGAAGG TGATCTCTCG GGTCTCTCTC 
GCCATCGCCC AGACCGCGGT CGATATCATC GAGAATTCGG CCACGTGGAG ATTCCCATCC 
GAGCTGGACC GCAGTGTCGT GGTGCGTGAT GTGGTGGGTA TCATGCAGAA GCTCCTTTCA 
TACACCTATA ACCTGAGCGC GAACGGGCCT TCGAATACCT TGACTGGCTC TCTTGAACCT 
GCCGCTAGAT ACGTTCTCGA AAGCTTTGTC ACCGCTTCGT CAAACTCACT CCGATTCCAA 
CCTCTTTTAG CGACACTTCT TGTTGCCTTC CAAATTCCTG ACTCGACTCT TTACCCCCGC 
CGGGCTGCCG TTGTCTCGGA GAGGCTCTTA GTTGTCCTTG ACTTCGCAAC CATCCTCCTC 
CGGGTTTCCA ACTACCTCGA TCTGCCTGTC AGCAAGCTCC AGACGCAGCT GTTCAAATCT 
GCGTCCCTGG TTGCCCGTCT TCCTGCTATC AGGCTGTCCT TCAAGATACC GGCGATAGCT 
CTTCTGAGCG CGCTGGTAGA AAGTGCCGGG AAGGGTAACG CCGAACCTCC ATCACTGCTG 
GGATACCTTG GGCCACAAAT TTCGCGGTCA TTCATCCAGA TTACTTCACA GCTGGATAAG 
CCGTTTGACA GGGTACCAGA GTCGGTCAGT ATATGGAAGT TCTTTTCAAC AATAATGCGG 
AATCGCCAAC AGTGGATGGC AAACTGCCTC CTTACGGGCA AGACGCCTCG CGAGGCCTTG 
AAGGGGGATG GCAAGATTGC AGAAATCTCT CCCGACTCGG TTCTTTCCAC GGCCATCGAA 
AAACTTCGCT CTATCACGAA GTTGCCAAGC CGGGAAGCCA TCGCCATTCT AGACTTTTTC 
ACCTCGGCCC AGAACTACTG GCCTTGGACG ATCTTCGCCA TGCAGAAGGA TACGTCCTTC 
CTGACCGACC TCCGAGCATA TGTCCGCGAT CTCAAGTCGC CCTCGATGGT CTCTAGGACA 
GACCCCAAGG AGGCTGCCTA CCAGGCGAGG ATAGCAGCTT ACATTGCCGA GGCATTTGCC 
ATGCAGCTGT ACCATCTGCG TCAGATGGGG CAATCACAAA AGTTTGCGAA TGACGTCGTG 
AACGATCTCG ATTACTTTTT GCGTAACGGA GTTGAGGTTT CGGGGTACAA CGCCTCGTTG 
CATTCTAACT TTGCAAAGAA CTTTAGCCAA CGGTACCCGG GGATTTCGGT AGAGGATTTC 
AAGAGGACAG TTCTTGTTCC CCGTGATCTG GGTTCCCAAT TTTACTATGC CTTGGATTTT 
GCCGACTTGA TGCTCAGTTA CGACGTGGCA TGGGATGGGT CGCGCAAGAA CGGCTTCAAG 
CATGAGATGG AGAGGGCTAA TGTGAACTTG TCTTTGGTGG AAGCTGAGAT TGCCCTTTTC 
CATGCTTGGG AGTATCTACT TCTAGAGCTT AGCGTCTCGC TTCTCCCCAA GAACGAAACC 
GTCGCCAAGC AGATGGCACA GGTCGCTGAG CAATGTCTGG ATGCCAACCA ACGGGCTCAG 
CCGCCAGACA ACATCTTCGT CCGCTTGGTT CACGCTCGGG CAAACCTGGC TTTGACGCTT 
CTTCAACGCC TCGCTGATGC TTCTCACCTG CCTAAGGACA GCACCAAGCT GCTCACCAGC 
ATCACAAGGG CCATCAATAA CATTGAGGTT CCCTTCGACA AGGATCAGAT CTCCTATTTC 
CGCACCCTCC TCAAGATCCT CTTCGTCGCT CTCCGTGGAA CCAGACACTC GGTCAATGCC 
CCTGCGCCCG GCGCCTCCAC CTTGTCGAAG TCCGGTGGTG AAAGCAGCTC CGTGGCCGTC 
ACCCAACTGA CTCTCAGCAT CCTTGACCGC GTCGTCGCCC AATCCTTCAG GTCCCTCGTC 
GCGCTAGTGC ACGAACCCAC CTCGCCCACC ACGCCCGAGG ACCTTGCCCT CATCACCGCG 
ATCCTGCAAG CCTGCCTGAG CGTCCCCGGC ATTGACCAGT GCCAGGTGCA AATCCTCAAC 
ATCATGGCCA GCCACGACGT GTTCCAAGTC GCCACCTCGC TCTTCTCCTG GGCCGACAAG 
CTGACGGACA AGTCTGGCGA CCCAATTTAC GGCGAGCTTT CCTTGCTCTT CCTGCTTGAG 
CTCTCCGCCA TGCCCGCCAT CGCGGAGCAG CTCGCCTGCG ACGGCCTGCT AGACCACATC 
ACGTCCGCCA ACCTTGCCGG CTTCATGCGC CGCCCCAACG TCGGCCCTCT AGCTGACAAC 
GCTGGCGCCG CCCGCTGCTA TTCCATATGG GCCAAGGGCA TCCTGCCTTT ACTACTCAAC 
ATGCTCGGTG CTTTGGGCGC CACCATCGCC CCTGAAATCG CTTACGTGCT GAATCAATTC 
CCCAATCTGT TGCGCGCGGC CGTGGATCGG TTCGAAGCAC CAGGCACCCA CCGCACCTTT 
TCGCCCTCCA AGTCCGTTTC TATCTCGGGT GGTCCCAACA ATAACAAGGA CATGTCTGGC 
TTCATCACCC TAACCGCCAT TTCGGAAATC CACTCCCTCG CCCTCCTCAC GCGCGTCCTG 
TCAGTCATCA GGATGACCAA TGTCGGCGGC CACTCGGCAA GAGACATCCC GCAGATTACC 
GGAGGGTGGG AAGACGAGGC CAGACAAAAT CTTTTGGAGA ATGTGGAGTT TTGGCTGGGG 
AGCAGGAAGG TGTTGAGGGA ACGGTTGGTG CCTTTGGGGC AGAGGGAGAT GGAGTGGAGG 
AGCCAGAAAG CTTCGGAAGG GTCAGCGTGC GAGAATAAGT TGGAAGAGAA GGCGGTGGAG 
ATGTTGAGTG GTGTGGCGGA GGTGTTGGCG TTGGGTGAGG CGGAGGAGGG TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU00199 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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