MGG_04235 cDNA ORF clone, Pyricularia oryzae 70-15

The following MGG_04235 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MGG_04235 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe02749 XM_003719531.1
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Pyricularia oryzae 70-15 uncharacterized protein (MGG_04235), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMe02749
    Clone ID Related Accession (Same CDS sequence) XM_003719531.1
    Accession Version XM_003719531.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-28
    Organism Pyricularia oryzae 70-15
    Product uncharacterized protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_017853).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCGCCGGT TTCTGGGCCG TCCCCCGATA GACCATGCGA ACGACTCCAT TACGAGCTCG 
    CCAAACCCCG CCCAACCCGT CGTCTACCGC CTCCACGCCT CACAAGATGC CGTGTTCCAA
    GCTGGCGTGC CAATCGCCGC TCTGGATCGG TCACCCGATG GCCGCTCAGC CGTGCTAGGC
    GGCCGTCACA TCTTGAAGAT TGTCCGCTTC GACGGTTGCA AAGTCGAAGA GCACGCCGAC
    CTGCGAGCAA GCTTGACTGG CCAGGCAGCA CGTGCAAACC CGAGCGCAAC AGCTTCGGTC
    GGAGATAAGC TCTCCATCAA GGATGTCAAA TGGGCTTCGT CCAGCGGCGG CGCCCAATCG
    CTCTACACGG CCTGCGGCAA CGGCAAGATT TTTCATTATG ACATTACCAG GTCTTTTGGA
    GCCATCGGCA GGCCCGGGGC GGCCCTAGAC ACCATCACGA TCCGCGAGGA CACGCGCCAG
    GTGAACGCGC TCGACGTGAA CCCTCACAGG GACTCGTGGC TTCTCTCCGG TAGCCAGGAC
    GGCTTTGTGC GGTGCTTTGA CACGCGCGAC CCCATATCGA TCAACCGCGG CGGTCTCACC
    TTTCGGCAGA TCCACGCCGG CAAGTGCAAC GATGGCGTGC GCCACGTCAA GTGGTCCCCA
    AAGGAGGGCT TCTGCTTCGC CTGCGGAACT GACAGCGGCG TCGTCATGAA GTGGGACAGC
    CGAATGTCTG CCAAGCCTGT GCTCCGGCTC AACGCCCACG AGGCGAACCG TGCCTGCACC
    ACCGTGGCGT GGCATCACGA CGGCGAGCAT CTTGCCAGCG CCGGTACGGA CAGCAAGCTG
    CACGTGTGGG ACGCTTCGCC AGGTGCCGAC AAGAAACAAA AGCCCAAGCT TTCCATCACG
    CTGCCAGCAC CGGCTGCCGC GATGACTTGG CGACCGGGTC AGTGGTCTGC CACGGTACAG
    GGACGGAGAG CCGCGCAGAT CGCAGTTTCC TACGACACTA GCAGTGCAGG GAAACGCCAT
    TCAAATGCCG TCGTTCATCT TTGGGACTTT GCCCGCCCGA CAATGCCATA CAGGGAGGTG
    GACCGATTTG ATGTCTCGCC AATGTCCCTC CTTTGGCTCG ATTCTGATCT GCTCTGGACA
    GCCGGCCCTG ATGGTATTTT CAGTCAGTGT GACGTTGCAT TTGCCCCCAA GGTCATTGAT
    CGGACTCCGG TCTCGGCAAT GGCTTTCTCT CCTCGCGGCG ATGTTGTTAT GCTGCTCGAT
    GAGCGGACCC AACCGCGTCG ACCTCGCCTG CCAGCCTCGC TCCATCGCAT GGATATGCGT
    CCGCCGCCAG CCGACTCTCC CAAAGAGCTA GTTGGCTCCA GCCCCGTTGC CAACATGTTC
    AGCATGGGTC GTAGCGATTC CGAGGACGAG GTAGTTACTA GCTTCCTCGG TCCCCGGAAA
    CCTGAAGCAC CGGCAGTGAG GAGCAGGCGA GCTAGCATCA AGTCATCTCC AAACACCGTC
    AGCACAACAC CGCCATCGAC AACCATCTCC AACGAGCCCG CTGTTGCCTT GGAGCCTTCA
    CTCAAGGTTG CAGGTATCTA TAAAAACCAC CAATCAATGG CCATCGGACA CGTCCCGGCT
    GCGCCTAGGG TTGACATTTA CCATTTCTTG TCTTCGCAAT ATCTCGAGAC TTTGGCAAAC
    GAACTGCCGT TTAATCCCGA GTCTGGCAGC AAACCTATGG TTGACCGAGT AGTCGGCATA
    CTGCGGCACT ACGCTCGAGC AGCCGATGCT GTCAGTCAAT TTCGCCTGGC ACAGACTTGG
    CGAATCCTTG CCTTTGCCAT GGACCTCCTT CTCAGAAGCA GGGCCCAATA TCACCTCGGT
    AGAAGAATGA ACCAGGCCAA GCTCAGGCTA GAATCCAGCA GAGAGGAACG TGCAAATGCC
    AAGGGAGGGG CACAGCCTCC TGCTAAGAAC CCAGTCACGA CCAACGAGCT CACGCCACGC
    CAGCAGTTTC CTGTGGGCAC ACCTCGCCCA CTGGCCAGGT CTTTACTGGC AGAAGAGTTT
    GAGAGCACCT CCAATGTGCC GACACCTTTG GCCAGGCCAG TCAGCGATGT TACTGCTCAT
    CAACGGAATC AAATCCATGA AAGCCCGACA AGCTTGCAGC ATGTCGGCGA CAGACTCATG
    TCTACGTCAG ATGTTGAGAA CCTGAGTCTT CCACCTGCTG TCCATTATCG TTCGGATTCG
    GATTCGCCAA TTCAGTCGCG TAAGCGTCTC GACTCGATCC CGTTGTCTGA GGTGAGCCAT
    GGCAGTGACC ACACACAACG TTCTATTACG GAGGGATACG ACTTTTACGA CATGGACGCC
    ATGGCAAAAG CAATGGATGT GCCTCATGGT TCCAGGAAGT CCTTGGGTTC AACACCACTC
    CCGTCTGATT ACGTGGAGCC CAAGTCGCCA GAGAGTCAAA AGCATCGGCC TGTGCTGAGA
    AAAGACTCGA CCGAAAGCAT TGGCAACGTT TTTTACTTTT CGCAGACCAG TGCCGGCAGT
    CTGCCCTCCA CGAGACAGTC AGTTAGTCCC GAACTAAAGG CCGGTGCTGT CCAGAGGGGT
    AGACGCCCAG CTCCTACTAG TGCGCCCTCT CAGCATCAAG GGAGCCAGAG TCCCGTGAGT
    GGGGAGTTGC ACAGTCGAAT ACGAGGCAGA GATCTGTCGG GCTCTCCCAA ATATCCCTCG
    TTGAAGCTCT TGCACCAGCA ACGTACAATG CGTCCGACAG GTGCAATCAA TACTCAGACT
    TCTGATGAGT ACAATGCAGA TCTTGTCCTG GACAGCCAAA CAACCCTGGA CACTCAATCT
    CCCCAGCGGA CTGCATCTGA TGGGCCTCCC CCCAGTTTTC CCGCTTCTGT CGATCCGCCC
    CTTCCAATCC CTAATCTGCG CACACCCAGC GAAGACGAAG AGTCCTCTAG TATCATTGAG
    GGTGACTATC TACCGTGGCA AGACGACCCC CCATACCCCT TTCCTACCCT ACAAGCTGGG
    CAGGAGTCAG ACCCACCACT CGATAACTTT CGCGCTACGC CCCTGCAACC ATATGCGCTC
    CTTGCTCGTG CGCTCGACTT TGAGAAGAAA CACTCCGCAC TGAACGCCTC TGCAATGATA
    CTATTACTCA GGCCTCTCGT ACCTGACGGG CTCATAGACG CCCATCTCGC CAATTCCATC
    CTGAGACAGC ATCACTCGCG GCTGATGGGC ATGAACCTAT TCGTTGAGGC GGCACTCCTA
    CGCAAGCTGT GTGTCAGGGG CTGGCCTGGA GGTGTCTTGG AGAATTACTG GGTCGAAGGA
    TACCCAGCCG TCTTTGCACA GGCGCAGCAG GGTGTCAGTG TTGCATACGT GTGCGGCAAG
    TGCAAGAGGC CACGCGAGAT GGACAGGTCG TCAACGTCAA GTGACACAGT ATGGAAGCCG
    TGCAAGAAGT GCCAGACCCC AGCAGGACCC TGTGCCATTT GCGGGCACCG TCATGTCACA
    GCTATTGTCG ATGAATACGA CATACCCGTA CCAGACGGAA ATGGGGGAGG AGGTGAAAAA
    GCCCAGGAGC AAGACAATGC GGGATCTGCC ACCAAACCCT CCAAGATGTC TCCCATTTCT
    ACATGGTGGT ACTGCCCCAC TTGCGCACAT GGCGGTCATT CTTCGTGCAT GTCCATATGG
    CACGGTGTTT CGCTAAACTC GCGACAGGCG GATGGCACAA CTGGACTTGT CGGTGGTGGG
    GCTGAATTGG GTGATGCATT TCCTTATTTG GCACCCGAGA TGATTCCAGG CGCCGGAATC
    TCGGATGGAT GCTGCCCTGT CGATGGATGC GGCCACGCCT GCCTCCCTGG CCGCTATCGC
    GCCGAGTCCA CAGCCGCGCG GACCGACGAG GTGAGCCGCG CTAGCCTGCG CGAGGCCGTG
    GCAGCCGCGA CGAGGGCTTC CTACTCTGCC GGGCCTAGCG CCCGGGGCTC ACCTGTCCTG
    CCACCCGGTG GGGGACAGCA ACCGCAGCAC TTGGCGGGTC TCAGCGATCT CAGCCTGGCC
    CTCGTCCGGG GTGACCCACA CGAGGCGCCA CAAAGCCGGG CCGTCGATGG CGTACGTGAT
    GCCCTCGGCG GACACCTCGA CCGCGCCTTT GCGGCCGGGG TCGCGGCCAG ACGGGGCGGG
    AGCATCTCGC TGAGCTCAAG CCCGGGGCGC GCCGGGGGCA TGCTTGGCGG CGGCCTTGGT
    CCCTCGGCCA GTGGAAACTT GCCATTTGGG CATGAGCGGG AGAGGAGAAA GAGCGTCAAA
    TTTGCAGGGG CCGACGAGAG GAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003719579.1
    CDS235..4521
    Translation

    Target ORF information:

    RefSeq Version XM_003719531.1
    Organism Pyricularia oryzae 70-15
    Definition Pyricularia oryzae 70-15 uncharacterized protein (MGG_04235), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003719531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCGCCGGT TTCTGGGCCG TCCCCCGATA GACCATGCGA ACGACTCCAT TACGAGCTCG 
    CCAAACCCCG CCCAACCCGT CGTCTACCGC CTCCACGCCT CACAAGATGC CGTGTTCCAA
    GCTGGCGTGC CAATCGCCGC TCTGGATCGG TCACCCGATG GCCGCTCAGC CGTGCTAGGC
    GGCCGTCACA TCTTGAAGAT TGTCCGCTTC GACGGTTGCA AAGTCGAAGA GCACGCCGAC
    CTGCGAGCAA GCTTGACTGG CCAGGCAGCA CGTGCAAACC CGAGCGCAAC AGCTTCGGTC
    GGAGATAAGC TCTCCATCAA GGATGTCAAA TGGGCTTCGT CCAGCGGCGG CGCCCAATCG
    CTCTACACGG CCTGCGGCAA CGGCAAGATT TTTCATTATG ACATTACCAG GTCTTTTGGA
    GCCATCGGCA GGCCCGGGGC GGCCCTAGAC ACCATCACGA TCCGCGAGGA CACGCGCCAG
    GTGAACGCGC TCGACGTGAA CCCTCACAGG GACTCGTGGC TTCTCTCCGG TAGCCAGGAC
    GGCTTTGTGC GGTGCTTTGA CACGCGCGAC CCCATATCGA TCAACCGCGG CGGTCTCACC
    TTTCGGCAGA TCCACGCCGG CAAGTGCAAC GATGGCGTGC GCCACGTCAA GTGGTCCCCA
    AAGGAGGGCT TCTGCTTCGC CTGCGGAACT GACAGCGGCG TCGTCATGAA GTGGGACAGC
    CGAATGTCTG CCAAGCCTGT GCTCCGGCTC AACGCCCACG AGGCGAACCG TGCCTGCACC
    ACCGTGGCGT GGCATCACGA CGGCGAGCAT CTTGCCAGCG CCGGTACGGA CAGCAAGCTG
    CACGTGTGGG ACGCTTCGCC AGGTGCCGAC AAGAAACAAA AGCCCAAGCT TTCCATCACG
    CTGCCAGCAC CGGCTGCCGC GATGACTTGG CGACCGGGTC AGTGGTCTGC CACGGTACAG
    GGACGGAGAG CCGCGCAGAT CGCAGTTTCC TACGACACTA GCAGTGCAGG GAAACGCCAT
    TCAAATGCCG TCGTTCATCT TTGGGACTTT GCCCGCCCGA CAATGCCATA CAGGGAGGTG
    GACCGATTTG ATGTCTCGCC AATGTCCCTC CTTTGGCTCG ATTCTGATCT GCTCTGGACA
    GCCGGCCCTG ATGGTATTTT CAGTCAGTGT GACGTTGCAT TTGCCCCCAA GGTCATTGAT
    CGGACTCCGG TCTCGGCAAT GGCTTTCTCT CCTCGCGGCG ATGTTGTTAT GCTGCTCGAT
    GAGCGGACCC AACCGCGTCG ACCTCGCCTG CCAGCCTCGC TCCATCGCAT GGATATGCGT
    CCGCCGCCAG CCGACTCTCC CAAAGAGCTA GTTGGCTCCA GCCCCGTTGC CAACATGTTC
    AGCATGGGTC GTAGCGATTC CGAGGACGAG GTAGTTACTA GCTTCCTCGG TCCCCGGAAA
    CCTGAAGCAC CGGCAGTGAG GAGCAGGCGA GCTAGCATCA AGTCATCTCC AAACACCGTC
    AGCACAACAC CGCCATCGAC AACCATCTCC AACGAGCCCG CTGTTGCCTT GGAGCCTTCA
    CTCAAGGTTG CAGGTATCTA TAAAAACCAC CAATCAATGG CCATCGGACA CGTCCCGGCT
    GCGCCTAGGG TTGACATTTA CCATTTCTTG TCTTCGCAAT ATCTCGAGAC TTTGGCAAAC
    GAACTGCCGT TTAATCCCGA GTCTGGCAGC AAACCTATGG TTGACCGAGT AGTCGGCATA
    CTGCGGCACT ACGCTCGAGC AGCCGATGCT GTCAGTCAAT TTCGCCTGGC ACAGACTTGG
    CGAATCCTTG CCTTTGCCAT GGACCTCCTT CTCAGAAGCA GGGCCCAATA TCACCTCGGT
    AGAAGAATGA ACCAGGCCAA GCTCAGGCTA GAATCCAGCA GAGAGGAACG TGCAAATGCC
    AAGGGAGGGG CACAGCCTCC TGCTAAGAAC CCAGTCACGA CCAACGAGCT CACGCCACGC
    CAGCAGTTTC CTGTGGGCAC ACCTCGCCCA CTGGCCAGGT CTTTACTGGC AGAAGAGTTT
    GAGAGCACCT CCAATGTGCC GACACCTTTG GCCAGGCCAG TCAGCGATGT TACTGCTCAT
    CAACGGAATC AAATCCATGA AAGCCCGACA AGCTTGCAGC ATGTCGGCGA CAGACTCATG
    TCTACGTCAG ATGTTGAGAA CCTGAGTCTT CCACCTGCTG TCCATTATCG TTCGGATTCG
    GATTCGCCAA TTCAGTCGCG TAAGCGTCTC GACTCGATCC CGTTGTCTGA GGTGAGCCAT
    GGCAGTGACC ACACACAACG TTCTATTACG GAGGGATACG ACTTTTACGA CATGGACGCC
    ATGGCAAAAG CAATGGATGT GCCTCATGGT TCCAGGAAGT CCTTGGGTTC AACACCACTC
    CCGTCTGATT ACGTGGAGCC CAAGTCGCCA GAGAGTCAAA AGCATCGGCC TGTGCTGAGA
    AAAGACTCGA CCGAAAGCAT TGGCAACGTT TTTTACTTTT CGCAGACCAG TGCCGGCAGT
    CTGCCCTCCA CGAGACAGTC AGTTAGTCCC GAACTAAAGG CCGGTGCTGT CCAGAGGGGT
    AGACGCCCAG CTCCTACTAG TGCGCCCTCT CAGCATCAAG GGAGCCAGAG TCCCGTGAGT
    GGGGAGTTGC ACAGTCGAAT ACGAGGCAGA GATCTGTCGG GCTCTCCCAA ATATCCCTCG
    TTGAAGCTCT TGCACCAGCA ACGTACAATG CGTCCGACAG GTGCAATCAA TACTCAGACT
    TCTGATGAGT ACAATGCAGA TCTTGTCCTG GACAGCCAAA CAACCCTGGA CACTCAATCT
    CCCCAGCGGA CTGCATCTGA TGGGCCTCCC CCCAGTTTTC CCGCTTCTGT CGATCCGCCC
    CTTCCAATCC CTAATCTGCG CACACCCAGC GAAGACGAAG AGTCCTCTAG TATCATTGAG
    GGTGACTATC TACCGTGGCA AGACGACCCC CCATACCCCT TTCCTACCCT ACAAGCTGGG
    CAGGAGTCAG ACCCACCACT CGATAACTTT CGCGCTACGC CCCTGCAACC ATATGCGCTC
    CTTGCTCGTG CGCTCGACTT TGAGAAGAAA CACTCCGCAC TGAACGCCTC TGCAATGATA
    CTATTACTCA GGCCTCTCGT ACCTGACGGG CTCATAGACG CCCATCTCGC CAATTCCATC
    CTGAGACAGC ATCACTCGCG GCTGATGGGC ATGAACCTAT TCGTTGAGGC GGCACTCCTA
    CGCAAGCTGT GTGTCAGGGG CTGGCCTGGA GGTGTCTTGG AGAATTACTG GGTCGAAGGA
    TACCCAGCCG TCTTTGCACA GGCGCAGCAG GGTGTCAGTG TTGCATACGT GTGCGGCAAG
    TGCAAGAGGC CACGCGAGAT GGACAGGTCG TCAACGTCAA GTGACACAGT ATGGAAGCCG
    TGCAAGAAGT GCCAGACCCC AGCAGGACCC TGTGCCATTT GCGGGCACCG TCATGTCACA
    GCTATTGTCG ATGAATACGA CATACCCGTA CCAGACGGAA ATGGGGGAGG AGGTGAAAAA
    GCCCAGGAGC AAGACAATGC GGGATCTGCC ACCAAACCCT CCAAGATGTC TCCCATTTCT
    ACATGGTGGT ACTGCCCCAC TTGCGCACAT GGCGGTCATT CTTCGTGCAT GTCCATATGG
    CACGGTGTTT CGCTAAACTC GCGACAGGCG GATGGCACAA CTGGACTTGT CGGTGGTGGG
    GCTGAATTGG GTGATGCATT TCCTTATTTG GCACCCGAGA TGATTCCAGG CGCCGGAATC
    TCGGATGGAT GCTGCCCTGT CGATGGATGC GGCCACGCCT GCCTCCCTGG CCGCTATCGC
    GCCGAGTCCA CAGCCGCGCG GACCGACGAG GTGAGCCGCG CTAGCCTGCG CGAGGCCGTG
    GCAGCCGCGA CGAGGGCTTC CTACTCTGCC GGGCCTAGCG CCCGGGGCTC ACCTGTCCTG
    CCACCCGGTG GGGGACAGCA ACCGCAGCAC TTGGCGGGTC TCAGCGATCT CAGCCTGGCC
    CTCGTCCGGG GTGACCCACA CGAGGCGCCA CAAAGCCGGG CCGTCGATGG CGTACGTGAT
    GCCCTCGGCG GACACCTCGA CCGCGCCTTT GCGGCCGGGG TCGCGGCCAG ACGGGGCGGG
    AGCATCTCGC TGAGCTCAAG CCCGGGGCGC GCCGGGGGCA TGCTTGGCGG CGGCCTTGGT
    CCCTCGGCCA GTGGAAACTT GCCATTTGGG CATGAGCGGG AGAGGAGAAA GAGCGTCAAA
    TTTGCAGGGG CCGACGAGAG GAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the rice blast fungus Magnaporthe grisea.
    Nature434(7036)980-6(2005 Apr)
    Dean RA,Talbot NJ,Ebbole DJ,Farman ML,Mitchell TK,Orbach MJ,Thon M,Kulkarni R,Xu JR,Pan H,Read ND,Lee YH,Carbone I,Brown D,Oh YY,Donofrio N,Jeong JS,Soanes DM,Djonovic S,Kolomiets E,Rehmeyer C,Li W,Harding M,Kim S,Lebrun MH,Bohnert H,Coughlan S,Butler J,Calvo S,Ma LJ,Nicol R,Purcell S,Nusbaum C,Galagan JE,Birren BW