MGG_03323 cDNA ORF clone, Pyricularia oryzae 70-15

The following MGG_03323 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MGG_03323 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe02229 XM_003716586.1
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Pyricularia oryzae 70-15 uncharacterized protein (MGG_03323), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMe02229
    Clone ID Related Accession (Same CDS sequence) XM_003716586.1
    Accession Version XM_003716586.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-28
    Organism Pyricularia oryzae 70-15
    Product uncharacterized protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_017851).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACAACT CGCGCCCGAA TGGCGCCTCC AATGGCGGCA TGTCTTACGG CAGAGAGATG 
    ACGCGGGCCG AGAGGTTCGA CGACGAGAAG CGCCGCCTCA TCGACAGTTG CTTCATGAAG
    AAGGACGAAG ATGGCTCCAT CATGGAGACG TACATCACGC ACATCAAGAT CACCGAATTC
    TCGACACATC CAACCACACC TCCGCCGCCG CAAGCTCGAA CCCCGCAAGT CGAGAAGCCG
    CGTGTCATCG CCGTGGCTGT GAGGAAATCT GGCAGAGTCC GGATGCATAA GACGAAGGAA
    AATGCTAATG GGACATTCTC CATCGGCAAA ACCTGGAATT TGGATGATCT GAGCGCTATC
    GAATCCTTCA CGGCTTCCTC GACCCCCCCA GACTACAGAC AGTGGGCCGG AGACGTTGGG
    TTCACCGTGA CACTAGGCAA GCCCTATTAT TGGCAAGCCC AGACTGACAA GGAAAAGAAG
    TTTTTCATTG CAAGTCTGAT CAAAATCTAT GGCAAGTATA CTGCTGGAAG AGTACCTGTG
    CTCACCGGAT TCGACCCCGC GGAGCTGGAA CAGGTCGTCG GGAGCGCCCA GCGCCGGCCA
    GGCCAGCCAC AGTTACAGCA GTTCTCACCT CAGCCGGCGA CCAATGCCTC GCCCATGCCC
    AATCGCTCGC CCGCCCCAGA ACGGTCTGAA CGAATACAGC CGCAGGAACG CCAGCCATAC
    GCCGGAAGAT CCGCCTTCCC AGACCGTGGC ACTGCACAAG AGCAAGTTTC ACCTCAAAAT
    GCCATGTCCA CTGCGTCAAG CAGCTATGGC CGTGAACGAC CCGACTACAG TCGTCCACCC
    CCACGAGGGC CACTGCGACA AGATGGGGCA TCATCGCCCT CTGCAAGTGT CAACTCTTCC
    AGGTCAGCCA ACCAAGACGC TCTACGACGA CTCGCAGGTG GAAACGTGAG CCAAGACTCG
    GTCGCAGGGA GGAGTGATGA CGGCAGCATC TCTACGACCA GGTCACGCGA AAGGCCTATG
    AACGGGGCTA CCTCTGCTTC AGCTGTTACG GAGCAGTCCC CTCGTGACGA GCGGCCTCCA
    GAGAGAAGGC GTCCGCCAGT GGAAGCGTCG CGCCAGCCGG TGCTTTCCCA GGGCATGACC
    GACGAGAGCT TAATGCCCGC GCCCTTGATG CGTCGTGACA GGGAGCCGAC CGTTCCACCC
    AGGAGTGTCG CGCGGAAACC GTCAGTTGCG TCACGTTCCG AAACAAGCTC ATTGTACGAC
    AGGTCTGCTC CCTCACAGGA ACAGAGCAGG CTCGAAGCAG TGCCGATGAA AAGTCCCGCG
    GCACCTTCCA GCCCCTTTTC AAAACCCCCA GAACCGAAGC CCGTCGTTGA GACACCAGCA
    CCCCCACCAC CAGAAGAACC GTCACCTATC TCGCCCGCAA CGCCCACCGA CTCTCCCGTG
    TCCCCAGAGG AGGAGACACG GCCTGGTCTC GGTCCGATGA TAAAGAAGAA GATGTCAAAG
    GGAGATGTCA AAGGGATGTT CTTCAAGGCT GCCTCGGCTG GAGGAGCCTT CCGGCCGCGC
    CCCGGTGGAG CTGGTGAGAG GTTGCGTTTA GCTGCGACCA AAGCCATGGC AGCTGAAGGT
    CCAGATGGTA TCACCGGTGT TGTACCAGCG CCTCCGAAGC CTGCTCCGGC CCCGGTCCCG
    GTCGAAAAAG AGAAAGAGAA GCCAGCACCA GCTTCATCTG TAGAGGCGTC GCCAATCAAC
    GAGATTCCTG CAGTCACGGT CACAGCATCT CAGTCAAGCC AGGCAACCAC CCTGGTGGCT
    GGTGGTGAGG CAAAGGGGCC CGAGGAACCC AAAGAGCAGA GTGAAGATGC GAAAAACCAA
    TCCAGCCGAT CTATCGTTGT CAGCAATGAT ATCAAGTACC TGACAAGCTT AGGGGTCGAT
    CCTGCGTTGT TGGATAACAG GAGTGTTGAG TTTAGCAAAT GGCTCGACCA TTTCAACTGG
    GTGCCAGGCG AGCAGATGCG GTCTAAGAAC TTTGACGAAT TGCGGATCGA CATTGATCGT
    GAGCTGAACA AGGCTCAGGC TGGCGGGTGG CTAGCTCGCT TCAAGGAGGA AGATGACAGA
    GTCGAGGGCA TCAAGAAGGG CATCGACCTG TCGATACAAG AATGCGAAGA GATGGACAAT
    CTTCTGACCT TGTACAGTGT TGAGTTATCA ACTTTATCGG ATGATATTGC ATACATTGAA
    GCACAAGGAC AAGGTCTGCA GGTGCAGGCG GCGAACCAAA AGCTGCTCAA GAAGGAGCTT
    GAATCACTCC TCGACACTTG TGCAATCACG ACTGATGACC TGGAGGCGCT CAAGAATACG
    CCTCTTGAGG ACCCTGATGG TCTTCACGAC ATTGAGACTG CCTTGGTCAC GCTCTTCAAA
    GCGATGATCA AGATTGACCC ATCACTTGGA AACCCTGGCA CCAAGAAATC TGAAGACGAT
    GGTGCTGATA CTGCCATGGG TCTCGATAGC GACTACGGCA AGATGCGCAT CGTCCAAGAA
    AAGAAGGAGA TGTATATGGC CGAAAGCAAG GCATTCATGC GGCGTCTGCT TCTGTTCACA
    GGCGATCGGT TCAAGGAAGC TTTCGACCAG ACTCGGAGGG CAGTGGAAGG ACCCACGTCC
    AAAAAAGCAG ATCCCCGTAA CCACGAAGTC GGGAGAGATA TGCTCTGGAT GTATAGCCCT
    TTGATGCTCT ACGCCAGGGA TGTGGACCTC CCTACGTGGG ATAGGATCAT TCACATCTAC
    CAGGAGACAA GTCTGCCGGT ATACAAGGAT GAATTCAGGG ATGCACTCGA CCGCTGGAAG
    ATCAACTCAA AGTCGGTTGG GGACGATGGC GACCTGCTCT TCACGTCTAT ACAAAGTGAG
    AAGCAACAAG AGGGTATTGC TGGCGCCGCG AGGAAGTTGA CGGTGAAGCG AAGCGGGACG
    TTGGCGAGAA CATTCCGCGG CGAAAGCAAA ACAAGCCTTG TCGAGAAACC TACTAATACG
    CATGGACAGC CATGGGAGGC GTTCTCGGGT GCTATGGATG ATCTTATGCC ACTGGTGGAA
    ATGGAGCAAA ATTTTATGAT CGACTTTTTC CACGCCACTA CGCTCGAGCA TCTTGACTTT
    CCTGATGCGG TGGCAGGATT CCGACCAAGA GAGCGACGAG GAGGCGATCT CAAGCGCCAT
    AGGATGAAGG AGCCCGATCG TGAATTGGCT AGGCGCGTCA CACGGGCGAT GGAAACTACA
    TTTGGCTTTT TGGAGGTCGA GGTCCAGAGG TTCATCGACT GGGTCCTGGC CAGCAACCCC
    ATTCAAGGAA TTGGTGTTCT AGCAACCCTG GAAAAGAAAG CCAGTGATCT TGGCACGTCA
    AACCAAGATT TCCTGATGAG CGTGCTGCAA AAATTGCAGG GTGTCCTCGA AGGTCGTTTC
    AAGCGATTCG TCGACGAGCA GATTCGCGCT ATTGAAGAGA CCAAGGTTAA GGTCAAGAAG
    CGAAAGGGAG TAGCACCGTT TATCCGCATC TTCCCCAACT TCAGCGCGGC GGTGGAGAAC
    ATGATGGCCG ACGTCGACAG CGAGCAGAAC GTACGCAAGC TGGTCGATCG TGAGTACGAA
    CGCATAATCA GAAGCATGTT CGACTCGGTG AAGATGATAG CGCGCGACAA CCCGTCGGTT
    TCGGCGGCAG CCAACAGCTC GCTTCCGGAT CCAGAGGACA AGGAGGCACT CAACTACCAC
    ATCTTGATGA TTGAAAACAT GAACTACTTC CTCGATGAGC TCAGCAAGTC TCACGGGCAG
    AGTTCGGGCG GCACGCAGGA GCCAGACGTA TTACAGCAGT GGAAGGAGAA AGCAAGCTCT
    GACTTGGCGG AGCACATGTC GATGTATCTG AACGCGGTGA TGCGAAGACC GCTGGGCAAA
    CTACTGGAGC ATCTCGAAAA CGTCGAGGCA CAGCTGGCGA GCGGCAAGAC GGCTAGCGCG
    GTGGCGGCGC AGCCCAGCAA CAGCAAGGCC ATCTTCAACA AGGTGCTTGG AAACTATGAC
    GCCAAGGAGG TGCGCAAGGG ACTCGAAGCG TTACGAAAGC GGGTCGAGAA ACACTTTGGT
    GACTCGGATG AAGGCAGTGG GGGCGCTGGG CCGGGCTACT CTCAGCAGCA TGAGCATACT
    CAGGGGCTGG TCAACAGGGT GCTGCGCGAG TGTGAGCGGT TCTACACTGA CGTGGGATCA
    CGTATCGAGA ACATCACGAC CAACGTATAC GGCGGGGATG TTTTGTTTGA GTGGCCAAGG
    GCGGAGGTAA AAGCTGCATT TTCGAACCTT GGGAGAGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003716634.1
    CDS441..4742
    Translation

    Target ORF information:

    RefSeq Version XM_003716586.1
    Organism Pyricularia oryzae 70-15
    Definition Pyricularia oryzae 70-15 uncharacterized protein (MGG_03323), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003716586.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACAACT CGCGCCCGAA TGGCGCCTCC AATGGCGGCA TGTCTTACGG CAGAGAGATG 
    ACGCGGGCCG AGAGGTTCGA CGACGAGAAG CGCCGCCTCA TCGACAGTTG CTTCATGAAG
    AAGGACGAAG ATGGCTCCAT CATGGAGACG TACATCACGC ACATCAAGAT CACCGAATTC
    TCGACACATC CAACCACACC TCCGCCGCCG CAAGCTCGAA CCCCGCAAGT CGAGAAGCCG
    CGTGTCATCG CCGTGGCTGT GAGGAAATCT GGCAGAGTCC GGATGCATAA GACGAAGGAA
    AATGCTAATG GGACATTCTC CATCGGCAAA ACCTGGAATT TGGATGATCT GAGCGCTATC
    GAATCCTTCA CGGCTTCCTC GACCCCCCCA GACTACAGAC AGTGGGCCGG AGACGTTGGG
    TTCACCGTGA CACTAGGCAA GCCCTATTAT TGGCAAGCCC AGACTGACAA GGAAAAGAAG
    TTTTTCATTG CAAGTCTGAT CAAAATCTAT GGCAAGTATA CTGCTGGAAG AGTACCTGTG
    CTCACCGGAT TCGACCCCGC GGAGCTGGAA CAGGTCGTCG GGAGCGCCCA GCGCCGGCCA
    GGCCAGCCAC AGTTACAGCA GTTCTCACCT CAGCCGGCGA CCAATGCCTC GCCCATGCCC
    AATCGCTCGC CCGCCCCAGA ACGGTCTGAA CGAATACAGC CGCAGGAACG CCAGCCATAC
    GCCGGAAGAT CCGCCTTCCC AGACCGTGGC ACTGCACAAG AGCAAGTTTC ACCTCAAAAT
    GCCATGTCCA CTGCGTCAAG CAGCTATGGC CGTGAACGAC CCGACTACAG TCGTCCACCC
    CCACGAGGGC CACTGCGACA AGATGGGGCA TCATCGCCCT CTGCAAGTGT CAACTCTTCC
    AGGTCAGCCA ACCAAGACGC TCTACGACGA CTCGCAGGTG GAAACGTGAG CCAAGACTCG
    GTCGCAGGGA GGAGTGATGA CGGCAGCATC TCTACGACCA GGTCACGCGA AAGGCCTATG
    AACGGGGCTA CCTCTGCTTC AGCTGTTACG GAGCAGTCCC CTCGTGACGA GCGGCCTCCA
    GAGAGAAGGC GTCCGCCAGT GGAAGCGTCG CGCCAGCCGG TGCTTTCCCA GGGCATGACC
    GACGAGAGCT TAATGCCCGC GCCCTTGATG CGTCGTGACA GGGAGCCGAC CGTTCCACCC
    AGGAGTGTCG CGCGGAAACC GTCAGTTGCG TCACGTTCCG AAACAAGCTC ATTGTACGAC
    AGGTCTGCTC CCTCACAGGA ACAGAGCAGG CTCGAAGCAG TGCCGATGAA AAGTCCCGCG
    GCACCTTCCA GCCCCTTTTC AAAACCCCCA GAACCGAAGC CCGTCGTTGA GACACCAGCA
    CCCCCACCAC CAGAAGAACC GTCACCTATC TCGCCCGCAA CGCCCACCGA CTCTCCCGTG
    TCCCCAGAGG AGGAGACACG GCCTGGTCTC GGTCCGATGA TAAAGAAGAA GATGTCAAAG
    GGAGATGTCA AAGGGATGTT CTTCAAGGCT GCCTCGGCTG GAGGAGCCTT CCGGCCGCGC
    CCCGGTGGAG CTGGTGAGAG GTTGCGTTTA GCTGCGACCA AAGCCATGGC AGCTGAAGGT
    CCAGATGGTA TCACCGGTGT TGTACCAGCG CCTCCGAAGC CTGCTCCGGC CCCGGTCCCG
    GTCGAAAAAG AGAAAGAGAA GCCAGCACCA GCTTCATCTG TAGAGGCGTC GCCAATCAAC
    GAGATTCCTG CAGTCACGGT CACAGCATCT CAGTCAAGCC AGGCAACCAC CCTGGTGGCT
    GGTGGTGAGG CAAAGGGGCC CGAGGAACCC AAAGAGCAGA GTGAAGATGC GAAAAACCAA
    TCCAGCCGAT CTATCGTTGT CAGCAATGAT ATCAAGTACC TGACAAGCTT AGGGGTCGAT
    CCTGCGTTGT TGGATAACAG GAGTGTTGAG TTTAGCAAAT GGCTCGACCA TTTCAACTGG
    GTGCCAGGCG AGCAGATGCG GTCTAAGAAC TTTGACGAAT TGCGGATCGA CATTGATCGT
    GAGCTGAACA AGGCTCAGGC TGGCGGGTGG CTAGCTCGCT TCAAGGAGGA AGATGACAGA
    GTCGAGGGCA TCAAGAAGGG CATCGACCTG TCGATACAAG AATGCGAAGA GATGGACAAT
    CTTCTGACCT TGTACAGTGT TGAGTTATCA ACTTTATCGG ATGATATTGC ATACATTGAA
    GCACAAGGAC AAGGTCTGCA GGTGCAGGCG GCGAACCAAA AGCTGCTCAA GAAGGAGCTT
    GAATCACTCC TCGACACTTG TGCAATCACG ACTGATGACC TGGAGGCGCT CAAGAATACG
    CCTCTTGAGG ACCCTGATGG TCTTCACGAC ATTGAGACTG CCTTGGTCAC GCTCTTCAAA
    GCGATGATCA AGATTGACCC ATCACTTGGA AACCCTGGCA CCAAGAAATC TGAAGACGAT
    GGTGCTGATA CTGCCATGGG TCTCGATAGC GACTACGGCA AGATGCGCAT CGTCCAAGAA
    AAGAAGGAGA TGTATATGGC CGAAAGCAAG GCATTCATGC GGCGTCTGCT TCTGTTCACA
    GGCGATCGGT TCAAGGAAGC TTTCGACCAG ACTCGGAGGG CAGTGGAAGG ACCCACGTCC
    AAAAAAGCAG ATCCCCGTAA CCACGAAGTC GGGAGAGATA TGCTCTGGAT GTATAGCCCT
    TTGATGCTCT ACGCCAGGGA TGTGGACCTC CCTACGTGGG ATAGGATCAT TCACATCTAC
    CAGGAGACAA GTCTGCCGGT ATACAAGGAT GAATTCAGGG ATGCACTCGA CCGCTGGAAG
    ATCAACTCAA AGTCGGTTGG GGACGATGGC GACCTGCTCT TCACGTCTAT ACAAAGTGAG
    AAGCAACAAG AGGGTATTGC TGGCGCCGCG AGGAAGTTGA CGGTGAAGCG AAGCGGGACG
    TTGGCGAGAA CATTCCGCGG CGAAAGCAAA ACAAGCCTTG TCGAGAAACC TACTAATACG
    CATGGACAGC CATGGGAGGC GTTCTCGGGT GCTATGGATG ATCTTATGCC ACTGGTGGAA
    ATGGAGCAAA ATTTTATGAT CGACTTTTTC CACGCCACTA CGCTCGAGCA TCTTGACTTT
    CCTGATGCGG TGGCAGGATT CCGACCAAGA GAGCGACGAG GAGGCGATCT CAAGCGCCAT
    AGGATGAAGG AGCCCGATCG TGAATTGGCT AGGCGCGTCA CACGGGCGAT GGAAACTACA
    TTTGGCTTTT TGGAGGTCGA GGTCCAGAGG TTCATCGACT GGGTCCTGGC CAGCAACCCC
    ATTCAAGGAA TTGGTGTTCT AGCAACCCTG GAAAAGAAAG CCAGTGATCT TGGCACGTCA
    AACCAAGATT TCCTGATGAG CGTGCTGCAA AAATTGCAGG GTGTCCTCGA AGGTCGTTTC
    AAGCGATTCG TCGACGAGCA GATTCGCGCT ATTGAAGAGA CCAAGGTTAA GGTCAAGAAG
    CGAAAGGGAG TAGCACCGTT TATCCGCATC TTCCCCAACT TCAGCGCGGC GGTGGAGAAC
    ATGATGGCCG ACGTCGACAG CGAGCAGAAC GTACGCAAGC TGGTCGATCG TGAGTACGAA
    CGCATAATCA GAAGCATGTT CGACTCGGTG AAGATGATAG CGCGCGACAA CCCGTCGGTT
    TCGGCGGCAG CCAACAGCTC GCTTCCGGAT CCAGAGGACA AGGAGGCACT CAACTACCAC
    ATCTTGATGA TTGAAAACAT GAACTACTTC CTCGATGAGC TCAGCAAGTC TCACGGGCAG
    AGTTCGGGCG GCACGCAGGA GCCAGACGTA TTACAGCAGT GGAAGGAGAA AGCAAGCTCT
    GACTTGGCGG AGCACATGTC GATGTATCTG AACGCGGTGA TGCGAAGACC GCTGGGCAAA
    CTACTGGAGC ATCTCGAAAA CGTCGAGGCA CAGCTGGCGA GCGGCAAGAC GGCTAGCGCG
    GTGGCGGCGC AGCCCAGCAA CAGCAAGGCC ATCTTCAACA AGGTGCTTGG AAACTATGAC
    GCCAAGGAGG TGCGCAAGGG ACTCGAAGCG TTACGAAAGC GGGTCGAGAA ACACTTTGGT
    GACTCGGATG AAGGCAGTGG GGGCGCTGGG CCGGGCTACT CTCAGCAGCA TGAGCATACT
    CAGGGGCTGG TCAACAGGGT GCTGCGCGAG TGTGAGCGGT TCTACACTGA CGTGGGATCA
    CGTATCGAGA ACATCACGAC CAACGTATAC GGCGGGGATG TTTTGTTTGA GTGGCCAAGG
    GCGGAGGTAA AAGCTGCATT TTCGAACCTT GGGAGAGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the rice blast fungus Magnaporthe grisea.
    Nature434(7036)980-6(2005 Apr)
    Dean RA,Talbot NJ,Ebbole DJ,Farman ML,Mitchell TK,Orbach MJ,Thon M,Kulkarni R,Xu JR,Pan H,Read ND,Lee YH,Carbone I,Brown D,Oh YY,Donofrio N,Jeong JS,Soanes DM,Djonovic S,Kolomiets E,Rehmeyer C,Li W,Harding M,Kim S,Lebrun MH,Bohnert H,Coughlan S,Butler J,Calvo S,Ma LJ,Nicol R,Purcell S,Nusbaum C,Galagan JE,Birren BW