WB; ICC/IF; IP;

Rabbit IgG

Unconjugated

Recombinant Protein of DYKDDDDK tag

E. coli

Protein G; G Protein

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Add 5 ml of distilled water to make the final concentration of 1 mg/ml

Protein G, a cell surface protein of group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A from Staphylococcus aureus. This product contains three IgG binding domains, but its albumin-binding region has been removed. Additionally, His-tag and cysteine have been added to N- and C-termini, respectively, to facilitate purification and conjugation. This does not affect the protein's ability to bind to IgG.

GenScript Ni Resin FF is a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by three coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. Ni Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. Ni Resin FF is an excellent choice for high performance purification of polyhistidine-tagged proteins.

SARS-CoV-2

CHO

Expressed with the mutations of E484K, K417N, N501Y.

Spike RBD (Arg319-Ser591) Accession # P0DTC2	Avi	Poly-His
N-term		C-term

Lineage P.1; 20J/501Y.V3; VOC-202101/02; Brazil(ian) variant; Gamma variant

WB;

Rabbit IgG

Unconjugated

Recombinant Protein of V5 tag

rTEV, TEV, P1 protease

Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques at GenScript.

GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins.

Human Rhinovirus

rPSP, PSP, HRV 3C Protease

Recombinant PreScission Protease (rPSP) from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. PSP is a highly purified recombinant 6xHis-fusion protein, which recognizes the same cleavage site as the native enzyme. It is used to cleave affinity tags from fusion proteins. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro(LEVLFQ/GP) and cleavage occurs between the Gln and Gly-Pro residues. The optimal temperature for cleavage is 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant PreScission Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant PreScission Protease (rPSP) contains 217 amino acids with N-terminal His tag. A fully biologically active molecule, rPSP has a molecular mass of 24 kDa and is obtained by proprietary chromatographic techniques at GenScript.

WB;

Rabbit IgG

Unconjugated

Recombinant Protein of GFP

WB; IHC; ICC/IF; IHF;

Rabbit IgG

Unconjugated

Recombinant Protein of GFP