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TEV Protease, His

TEV Protease, His

Lane 1: 2 μg of TEV Protease, His, non-reducing (NR)
Lane 2: 2 μg of TEV Protease, His, reducing (R)
> 95% as analyzed by SDS-PAGE

TEV Protease, His

The fusion protein (20 μg) was incubated with varying enzyme doses (2U, 3U, 4U, 5U) in a 30°C water bath for 1 hour.

TEV Protease, His

Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques at GenScript.
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Description

Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the tags from fusion proteins. The optimal temperature for cleavage is 30°C; also it can be used at temperature as low as 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
Recombinant Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques at GenScript.
Synonyms

rTEV, TEV, P1 protease

Note

For research use only

Overview
Source E. coli

Properties
Purity > 95% by SDS-PAGE analyses.
Biological Activity 10 IU/μl. Unit Definition: One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.
Formulation Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH7.5.
Measured Molecular Weight 28.4 kDa, observed by reducing SDS-PAGE.
Storage & Stability Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20°C from date of receipt. Please avoid freeze-thaw cycles.

Examples
  • TEV Protease, His
    • TEV Protease, His

  • TEV Protease, His
    • TEV Protease, His

    Lane 1: 2 μg of TEV Protease, His, non-reducing (NR)
    Lane 2: 2 μg of TEV Protease, His, reducing (R)
    > 95% as analyzed by SDS-PAGE

  • TEV Protease, His
    • TEV Protease, His

    The fusion protein (20 μg) was incubated with varying enzyme doses (2U, 3U, 4U, 5U) in a 30°C water bath for 1 hour.