GenCRISPR™ ゲノム編集関連製品 » GenCRISPR™ ヌクレアーゼ-CRISPR遺伝子編集 » GenCrispr Mutation Detection Kit

GenCrispr Mutation Detection Kit

GenCrispr Mutation Detection Kit
L00688
¥25,200.00

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Description

The GenCrispr Mutation Detection Kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic DNA that is extracted from cells transfected with constructs expressing engineered nucleases such as Transcription activator-like effector nucleases (TALEN), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, or Zinc-finger nucleases (ZFN). The GenCrispr Mutation Detection Kit includes High-Fidelity DNA polymerase for amplifying the target regions from cells, and GenCrispr T7 Endonuclease I for recognizing and detecting the mismatches caused by gene editing tools. It provides an easy and reliable approach for estimating the efficiency of genome editing.

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ComponentsAmount provided
25-reaction kit100-reaction kit
High-Fidelity DNA polymerase10 μL40 μL
5X PCR Reaction Buffer150 μL600 μL
GenCrispr T7 Endonuclease I (Cat.No.Z03396)25 μL100 μL
10X GenCrispr T7 Endonuclease I Reaction buffer50 μL500 μL
Control Template DNA30 μL120 μL
Control Primer Mix30 μL120 μL
Protease K25 μL100 μL
dNTP15 μL60 μL

Note

Research purposes only.

Product Introduction
Storage Store all components at -20°C upon receipt.

Applications
Detect the gene mutagenesis and SNP, for cleavage efficiency assay caused by ZFNs, TALENs, CRISPR/Cas9 or other gene editing tools.